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BR9909147A - Serine protease variants with amino acid deletions and substitutions - Google Patents

Serine protease variants with amino acid deletions and substitutions

Info

Publication number
BR9909147A
BR9909147A BR9909147-0A BR9909147A BR9909147A BR 9909147 A BR9909147 A BR 9909147A BR 9909147 A BR9909147 A BR 9909147A BR 9909147 A BR9909147 A BR 9909147A
Authority
BR
Brazil
Prior art keywords
amino acid
variants
substitutions
serine protease
acid sequence
Prior art date
Application number
BR9909147-0A
Other languages
Portuguese (pt)
Inventor
Donn Nelton Rubingh
Elizabeth Ellen Sikorski
Paul Elliott Correa
Original Assignee
Procter & Gamble
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Procter & Gamble filed Critical Procter & Gamble
Publication of BR9909147A publication Critical patent/BR9909147A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
    • A61K8/0208—Tissues; Wipes; Patches
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
    • A61K8/66—Enzymes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/86—Products or compounds obtained by genetic engineering
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q11/00—Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/10—Washing or bathing preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q5/00—Preparations for care of the hair

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Birds (AREA)
  • Epidemiology (AREA)
  • Dermatology (AREA)
  • Detergent Compositions (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Cosmetics (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

Patente de Invenção: <B>"VARIANTES DE SERINA PROTEASE COM DELEçõES E SUBSTITUIçõES DE AMINOáCIDOS"<D>. A presente invenção refere-se a variantes de serina proteases com imunogenicidade diminuída com relação a suas proteases de tipo selvagem correspondentes. Mais particularmente, a presente invenção se refere a variantes com uma seq³ência de aminoácidos engenheirada de uma seq³ência de aminoácidos do tipo selvagem, em que a seq³ência de aminoácidos engenheirada compreende uma deleção e, opcionalmente, uma substituição de uma ou mais posições especificamente identificadas, que correspondam à subtilisina BPN'. A invenção também se refere a genes mutantes que codificam essas variantes e a composições de limpeza e para cuidados pessoais compreendendo essas variantes.Invention Patent: <B> "SERINE PROTEASE VARIANTS WITH AMINO ACID DELETIONS AND SUBSTITUTIONS" <D>. The present invention relates to variants of serine proteases with decreased immunogenicity with respect to their corresponding wild type proteases. More particularly, the present invention relates to variants with an engineered amino acid sequence of a wild type amino acid sequence, wherein the engineered amino acid sequence comprises a deletion and, optionally, a replacement of one or more specifically identified positions, which correspond to subtilisin BPN '. The invention also relates to mutant genes that encode these variants and to cleaning and personal care compositions comprising these variants.

BR9909147-0A 1998-03-26 1999-03-25 Serine protease variants with amino acid deletions and substitutions BR9909147A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US7947798P 1998-03-26 1998-03-26
PCT/IB1999/000520 WO1999049057A1 (en) 1998-03-26 1999-03-25 Serine protease variants having amino acid deletions and substitutions

Publications (1)

Publication Number Publication Date
BR9909147A true BR9909147A (en) 2000-12-05

Family

ID=22150818

Family Applications (1)

Application Number Title Priority Date Filing Date
BR9909147-0A BR9909147A (en) 1998-03-26 1999-03-25 Serine protease variants with amino acid deletions and substitutions

Country Status (9)

Country Link
EP (1) EP1082443A1 (en)
JP (1) JP2002518993A (en)
KR (1) KR20010052223A (en)
CN (1) CN1303437A (en)
AR (1) AR023021A1 (en)
AU (1) AU750336B2 (en)
BR (1) BR9909147A (en)
CA (1) CA2324422A1 (en)
WO (1) WO1999049057A1 (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001007575A2 (en) * 1999-07-22 2001-02-01 The Procter & Gamble Company Subtilisin protease variants having amino acid deletions and substitutions in defined epitope regions
US6803222B2 (en) 2000-11-22 2004-10-12 Kao Corporation Alkaline proteases
DE10360805A1 (en) 2003-12-23 2005-07-28 Henkel Kgaa New alkaline protease and detergents containing this novel alkaline protease
DE102007003143A1 (en) 2007-01-16 2008-07-17 Henkel Kgaa New alkaline protease from Bacillus gibsonii and detergents and cleaners containing this novel alkaline protease
DE102007032111B4 (en) 2007-07-09 2017-07-20 Henkel Ag & Co. Kgaa New proteases and detergents and cleaning agents containing these proteases
DE102007036756A1 (en) 2007-08-03 2009-02-05 Henkel Ag & Co. Kgaa New proteases and detergents and cleaners containing these new proteases
DE102007051092A1 (en) 2007-10-24 2009-04-30 Henkel Ag & Co. Kgaa Subtilisin from Becillus pumilus and detergents and cleaners containing this new subtilisin
JP2012524542A (en) * 2009-04-24 2012-10-18 ダニスコ・ユーエス・インク Protease with modified pro (PRO) region
EP2480663B1 (en) * 2009-09-25 2017-11-15 Novozymes A/S Use of variants of the serin protease subtilisin
CN103958657A (en) * 2011-11-25 2014-07-30 诺维信公司 Subtilase variants and polynucleotides encoding same
CN102839165B (en) * 2012-09-26 2014-12-10 金普诺安生物科技（苏州）有限公司 Gene mutation type recombined protease K and industrialized production method thereof
EP4291647A1 (en) * 2021-02-12 2023-12-20 Fresenius Kabi iPSUM S.r.l. Subtilisin variants and their use

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0353250B1 (en) * 1987-04-06 1999-09-08 Novo Nordisk A/S The engineering of electrostatic interactions at metal ion binding sites for the stabilization of proteins
EP0405901B1 (en) * 1989-06-26 2004-09-01 Unilever Plc Enzymatic detergent compositions
KR100237968B1 (en) * 1990-12-05 2000-01-15 한센 핀 베네드 Proteins with changed epitopes and methods for the production thereof
US5567601A (en) * 1993-06-01 1996-10-22 University Of Maryland Subtilisin mutants lacking a primary calcium binding site
JP2002507426A (en) * 1998-03-26 2002-03-12 ザ、プロクター、エンド、ギャンブル、カンパニー Serine protease variants with amino acid substitutions

Also Published As

Publication number Publication date
CN1303437A (en) 2001-07-11
AU750336B2 (en) 2002-07-18
EP1082443A1 (en) 2001-03-14
AU2742699A (en) 1999-10-18
CA2324422A1 (en) 1999-09-30
KR20010052223A (en) 2001-06-25
AR023021A1 (en) 2002-09-04
JP2002518993A (en) 2002-07-02
WO1999049057A1 (en) 1999-09-30

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Legal Events

Date Code Title Description
B08F Application fees: application dismissed [chapter 8.6 patent gazette]

Free format text: REFERENTE A 8A,9A E 10A ANUIDADES.

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: REFERENTE AO DESPACHO PUBLICADO NA RPI 1990 DE 25/02/2009.

B15K Others concerning applications: alteration of classification

Ipc: C12N 9/54 (2006.01), A61K 8/02 (2006.0