BR112022010153A2 - Peptídeos, ácido nucleico, polipeptídeo, composição, complexo bioluminescente, métodos, dipeptídeo, sistema e kit - Google Patents
Peptídeos, ácido nucleico, polipeptídeo, composição, complexo bioluminescente, métodos, dipeptídeo, sistema e kitInfo
- Publication number
- BR112022010153A2 BR112022010153A2 BR112022010153A BR112022010153A BR112022010153A2 BR 112022010153 A2 BR112022010153 A2 BR 112022010153A2 BR 112022010153 A BR112022010153 A BR 112022010153A BR 112022010153 A BR112022010153 A BR 112022010153A BR 112022010153 A2 BR112022010153 A2 BR 112022010153A2
- Authority
- BR
- Brazil
- Prior art keywords
- methods
- polypeptide
- dipeptide
- peptides
- kit
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
- G01N21/763—Bioluminescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
- C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
- C12Y113/12005—Renilla-luciferin 2-monooxygenase (1.13.12.5), i.e. renilla-luciferase
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Plasma & Fusion (AREA)
- Plant Pathology (AREA)
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Cosmetics (AREA)
Abstract
PEPTÍDEOS, ÁCIDO NUCLEICO, POLIPEPTÍDEO, COMPOSIÇÃO, COMPLEXO BIOLUMINESCENTE, MÉTODOS, DIPEPTÍDEO, SISTEMA E KIT. São fornecidos neste documento polipeptídeos bioluminescentes e composições e métodos para a montagem de um complexo bioluminescente tripartite ou multipartite. Em modalidades particulares, um complexo bioluminescente é formado mediante a interação de três ou mais componentes de peptídeo e/ou polipeptídeo.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201962941255P | 2019-11-27 | 2019-11-27 | |
PCT/US2020/062499 WO2021108765A1 (en) | 2019-11-27 | 2020-11-27 | Multipartite luciferase peptides and polypeptides |
Publications (1)
Publication Number | Publication Date |
---|---|
BR112022010153A2 true BR112022010153A2 (pt) | 2022-08-09 |
Family
ID=74106118
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR112022010153A BR112022010153A2 (pt) | 2019-11-27 | 2020-11-27 | Peptídeos, ácido nucleico, polipeptídeo, composição, complexo bioluminescente, métodos, dipeptídeo, sistema e kit |
Country Status (10)
Country | Link |
---|---|
US (1) | US20210262941A1 (pt) |
EP (1) | EP4065699A1 (pt) |
JP (1) | JP2023503653A (pt) |
KR (1) | KR20220121816A (pt) |
CN (1) | CN115427556A (pt) |
AU (1) | AU2020392245A1 (pt) |
BR (1) | BR112022010153A2 (pt) |
CA (1) | CA3158729A1 (pt) |
IL (1) | IL293204A (pt) |
WO (1) | WO2021108765A1 (pt) |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2020191339A1 (en) | 2019-03-20 | 2020-09-24 | Promega Corporation | Photoaffinity probes |
US20240003881A1 (en) * | 2020-12-04 | 2024-01-04 | The Governing Council Of The University Of Toronto | System and method for rapid and sensitive detection of anti-pathogen antibodies |
WO2023039535A1 (en) * | 2021-09-09 | 2023-03-16 | Trustees Of Tufts College | Outer membrane vesicle (omv)-based vaccines against sudden acute respiratory syndrome coronavirus 2 (sars-cov-2) spike protein |
US20230357266A1 (en) | 2022-05-04 | 2023-11-09 | Promega Corporation | Photoactivatable compounds and uses thereof |
GB2619059A (en) * | 2022-05-26 | 2023-11-29 | Univ Leeds Innovations Ltd | Split reporter complex |
Family Cites Families (26)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5260203A (en) | 1986-09-02 | 1993-11-09 | Enzon, Inc. | Single polypeptide chain binding molecules |
US4946778A (en) | 1987-09-21 | 1990-08-07 | Genex Corporation | Single polypeptide chain binding molecules |
WO1988001649A1 (en) | 1986-09-02 | 1988-03-10 | Genex Corporation | Single polypeptide chain binding molecules |
US5914095A (en) | 1989-04-07 | 1999-06-22 | Salutar, Inc. | Polychelants containg amide bonds |
US5368484A (en) | 1992-05-22 | 1994-11-29 | Atari Games Corp. | Vehicle simulator with realistic operating feedback |
CA2146990A1 (en) | 1992-10-14 | 1994-04-28 | Robert A. Snow | Chelating polymers |
GB9503969D0 (en) | 1995-02-28 | 1995-04-19 | Sams Bernard | Incrementing mechanism |
US7268229B2 (en) | 2001-11-02 | 2007-09-11 | Promega Corporation | Compounds to co-localize luminophores with luminescent proteins |
SG10201601281WA (en) | 2009-05-01 | 2016-03-30 | Promega Corp | Synthetic oplophorus luciferases with enhanced light output |
TW201105646A (en) | 2009-05-28 | 2011-02-16 | Solvay Fluor Gmbh | Process for the preparation of 4-fluoro-4-R-5-R'-1,3-dioxolane-2-ones |
ES2723773T3 (es) | 2010-11-02 | 2019-09-02 | Promega Corp | Luciferasas derivadas de Oplophorus, nuevos sustratos de celenterazina y procedimientos de uso |
WO2013177255A2 (en) * | 2012-05-24 | 2013-11-28 | Connecticut College | Chimeric luciferases |
DE202013012241U1 (de) | 2012-05-25 | 2016-01-18 | Emmanuelle Charpentier | Zusammensetzungen für die durch RNA gesteuerte Modifikation einer Ziel-DNA und für die durch RNA gesteuerte Modulation der Transkription |
US20140099654A1 (en) | 2012-09-26 | 2014-04-10 | Promega Corporation | Real-time monitoring |
US8697359B1 (en) | 2012-12-12 | 2014-04-15 | The Broad Institute, Inc. | CRISPR-Cas systems and methods for altering expression of gene products |
PT2970412T (pt) | 2013-03-15 | 2022-09-13 | Promega Corp | Ativação de bioluminescência por complementação estrutural |
US11365402B2 (en) * | 2014-09-12 | 2022-06-21 | Promega Corporation | Internal protein tags |
EP4455668A2 (en) * | 2015-02-05 | 2024-10-30 | Promega Corporation | Luciferase-based thermal shift assays |
US9908918B2 (en) | 2015-06-30 | 2018-03-06 | The Board Of Trustees Of The Leland Stanford Junior University | Cyan-excitable orange-red fluorescent proteins and bioluminescent resonance energy transfer systems |
US9924073B2 (en) | 2016-02-15 | 2018-03-20 | Promega Corporation | Substituted imidazo[1,2-a]pyrazines as luciferase substrates and preparation method thereof |
WO2017189751A1 (en) * | 2016-04-26 | 2017-11-02 | The University Of Utah Research Foundation | Target-binding activated split reporter systems for analyte detection and related components and methods |
DK3490991T3 (da) | 2016-07-28 | 2021-01-25 | Promega Corp | Coelenterazine analogues |
DK3759233T3 (da) * | 2018-02-26 | 2022-07-11 | Univ Eindhoven Tech | Bioluminescerende biosensor til detektering og kvantificering af biomolekyler |
CN112567028A (zh) * | 2018-06-12 | 2021-03-26 | 普洛麦格公司 | 多部分萤光素酶 |
US10962538B2 (en) * | 2018-06-14 | 2021-03-30 | The Trustees Of Columbia University In The City Of New York | Assays using arrestin recruitment and unmodified receptors |
KR20220007058A (ko) * | 2019-04-10 | 2022-01-18 | 프로메가 코포레이션 | 생물발광을 이용하는 분석물 검출을 위한 조성물 및 방법 |
-
2020
- 2020-11-27 EP EP20834006.7A patent/EP4065699A1/en active Pending
- 2020-11-27 CA CA3158729A patent/CA3158729A1/en active Pending
- 2020-11-27 BR BR112022010153A patent/BR112022010153A2/pt unknown
- 2020-11-27 KR KR1020227021856A patent/KR20220121816A/ko unknown
- 2020-11-27 JP JP2022531486A patent/JP2023503653A/ja active Pending
- 2020-11-27 US US17/105,925 patent/US20210262941A1/en active Pending
- 2020-11-27 AU AU2020392245A patent/AU2020392245A1/en active Pending
- 2020-11-27 CN CN202080095195.6A patent/CN115427556A/zh active Pending
- 2020-11-27 IL IL293204A patent/IL293204A/en unknown
- 2020-11-27 WO PCT/US2020/062499 patent/WO2021108765A1/en unknown
Also Published As
Publication number | Publication date |
---|---|
EP4065699A1 (en) | 2022-10-05 |
JP2023503653A (ja) | 2023-01-31 |
US20210262941A1 (en) | 2021-08-26 |
CA3158729A1 (en) | 2021-06-03 |
CN115427556A (zh) | 2022-12-02 |
AU2020392245A1 (en) | 2022-06-23 |
WO2021108765A1 (en) | 2021-06-03 |
KR20220121816A (ko) | 2022-09-01 |
IL293204A (en) | 2022-07-01 |
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