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BR112022010153A2 - Peptídeos, ácido nucleico, polipeptídeo, composição, complexo bioluminescente, métodos, dipeptídeo, sistema e kit - Google Patents

Peptídeos, ácido nucleico, polipeptídeo, composição, complexo bioluminescente, métodos, dipeptídeo, sistema e kit

Info

Publication number
BR112022010153A2
BR112022010153A2 BR112022010153A BR112022010153A BR112022010153A2 BR 112022010153 A2 BR112022010153 A2 BR 112022010153A2 BR 112022010153 A BR112022010153 A BR 112022010153A BR 112022010153 A BR112022010153 A BR 112022010153A BR 112022010153 A2 BR112022010153 A2 BR 112022010153A2
Authority
BR
Brazil
Prior art keywords
methods
polypeptide
dipeptide
peptides
kit
Prior art date
Application number
BR112022010153A
Other languages
English (en)
Inventor
Kincaid Virginia
P Encell Lance
Hall Mary
Killoran Michael
Wood Keith
Dart Melanie
Hoang Trish
Original Assignee
Promega Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Promega Corp filed Critical Promega Corp
Publication of BR112022010153A2 publication Critical patent/BR112022010153A2/pt

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0069Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62DNA sequences coding for fusion proteins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/76Chemiluminescence; Bioluminescence
    • G01N21/763Bioluminescence
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/61Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y113/00Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/12Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
    • C12Y113/12005Renilla-luciferin 2-monooxygenase (1.13.12.5), i.e. renilla-luciferase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Plasma & Fusion (AREA)
  • Plant Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Cosmetics (AREA)

Abstract

PEPTÍDEOS, ÁCIDO NUCLEICO, POLIPEPTÍDEO, COMPOSIÇÃO, COMPLEXO BIOLUMINESCENTE, MÉTODOS, DIPEPTÍDEO, SISTEMA E KIT. São fornecidos neste documento polipeptídeos bioluminescentes e composições e métodos para a montagem de um complexo bioluminescente tripartite ou multipartite. Em modalidades particulares, um complexo bioluminescente é formado mediante a interação de três ou mais componentes de peptídeo e/ou polipeptídeo.
BR112022010153A 2019-11-27 2020-11-27 Peptídeos, ácido nucleico, polipeptídeo, composição, complexo bioluminescente, métodos, dipeptídeo, sistema e kit BR112022010153A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962941255P 2019-11-27 2019-11-27
PCT/US2020/062499 WO2021108765A1 (en) 2019-11-27 2020-11-27 Multipartite luciferase peptides and polypeptides

Publications (1)

Publication Number Publication Date
BR112022010153A2 true BR112022010153A2 (pt) 2022-08-09

Family

ID=74106118

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112022010153A BR112022010153A2 (pt) 2019-11-27 2020-11-27 Peptídeos, ácido nucleico, polipeptídeo, composição, complexo bioluminescente, métodos, dipeptídeo, sistema e kit

Country Status (10)

Country Link
US (1) US20210262941A1 (pt)
EP (1) EP4065699A1 (pt)
JP (1) JP2023503653A (pt)
KR (1) KR20220121816A (pt)
CN (1) CN115427556A (pt)
AU (1) AU2020392245A1 (pt)
BR (1) BR112022010153A2 (pt)
CA (1) CA3158729A1 (pt)
IL (1) IL293204A (pt)
WO (1) WO2021108765A1 (pt)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020191339A1 (en) 2019-03-20 2020-09-24 Promega Corporation Photoaffinity probes
US20240003881A1 (en) * 2020-12-04 2024-01-04 The Governing Council Of The University Of Toronto System and method for rapid and sensitive detection of anti-pathogen antibodies
WO2023039535A1 (en) * 2021-09-09 2023-03-16 Trustees Of Tufts College Outer membrane vesicle (omv)-based vaccines against sudden acute respiratory syndrome coronavirus 2 (sars-cov-2) spike protein
US20230357266A1 (en) 2022-05-04 2023-11-09 Promega Corporation Photoactivatable compounds and uses thereof
GB2619059A (en) * 2022-05-26 2023-11-29 Univ Leeds Innovations Ltd Split reporter complex

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US5260203A (en) 1986-09-02 1993-11-09 Enzon, Inc. Single polypeptide chain binding molecules
US4946778A (en) 1987-09-21 1990-08-07 Genex Corporation Single polypeptide chain binding molecules
WO1988001649A1 (en) 1986-09-02 1988-03-10 Genex Corporation Single polypeptide chain binding molecules
US5914095A (en) 1989-04-07 1999-06-22 Salutar, Inc. Polychelants containg amide bonds
US5368484A (en) 1992-05-22 1994-11-29 Atari Games Corp. Vehicle simulator with realistic operating feedback
CA2146990A1 (en) 1992-10-14 1994-04-28 Robert A. Snow Chelating polymers
GB9503969D0 (en) 1995-02-28 1995-04-19 Sams Bernard Incrementing mechanism
US7268229B2 (en) 2001-11-02 2007-09-11 Promega Corporation Compounds to co-localize luminophores with luminescent proteins
SG10201601281WA (en) 2009-05-01 2016-03-30 Promega Corp Synthetic oplophorus luciferases with enhanced light output
TW201105646A (en) 2009-05-28 2011-02-16 Solvay Fluor Gmbh Process for the preparation of 4-fluoro-4-R-5-R'-1,3-dioxolane-2-ones
ES2723773T3 (es) 2010-11-02 2019-09-02 Promega Corp Luciferasas derivadas de Oplophorus, nuevos sustratos de celenterazina y procedimientos de uso
WO2013177255A2 (en) * 2012-05-24 2013-11-28 Connecticut College Chimeric luciferases
DE202013012241U1 (de) 2012-05-25 2016-01-18 Emmanuelle Charpentier Zusammensetzungen für die durch RNA gesteuerte Modifikation einer Ziel-DNA und für die durch RNA gesteuerte Modulation der Transkription
US20140099654A1 (en) 2012-09-26 2014-04-10 Promega Corporation Real-time monitoring
US8697359B1 (en) 2012-12-12 2014-04-15 The Broad Institute, Inc. CRISPR-Cas systems and methods for altering expression of gene products
PT2970412T (pt) 2013-03-15 2022-09-13 Promega Corp Ativação de bioluminescência por complementação estrutural
US11365402B2 (en) * 2014-09-12 2022-06-21 Promega Corporation Internal protein tags
EP4455668A2 (en) * 2015-02-05 2024-10-30 Promega Corporation Luciferase-based thermal shift assays
US9908918B2 (en) 2015-06-30 2018-03-06 The Board Of Trustees Of The Leland Stanford Junior University Cyan-excitable orange-red fluorescent proteins and bioluminescent resonance energy transfer systems
US9924073B2 (en) 2016-02-15 2018-03-20 Promega Corporation Substituted imidazo[1,2-a]pyrazines as luciferase substrates and preparation method thereof
WO2017189751A1 (en) * 2016-04-26 2017-11-02 The University Of Utah Research Foundation Target-binding activated split reporter systems for analyte detection and related components and methods
DK3490991T3 (da) 2016-07-28 2021-01-25 Promega Corp Coelenterazine analogues
DK3759233T3 (da) * 2018-02-26 2022-07-11 Univ Eindhoven Tech Bioluminescerende biosensor til detektering og kvantificering af biomolekyler
CN112567028A (zh) * 2018-06-12 2021-03-26 普洛麦格公司 多部分萤光素酶
US10962538B2 (en) * 2018-06-14 2021-03-30 The Trustees Of Columbia University In The City Of New York Assays using arrestin recruitment and unmodified receptors
KR20220007058A (ko) * 2019-04-10 2022-01-18 프로메가 코포레이션 생물발광을 이용하는 분석물 검출을 위한 조성물 및 방법

Also Published As

Publication number Publication date
EP4065699A1 (en) 2022-10-05
JP2023503653A (ja) 2023-01-31
US20210262941A1 (en) 2021-08-26
CA3158729A1 (en) 2021-06-03
CN115427556A (zh) 2022-12-02
AU2020392245A1 (en) 2022-06-23
WO2021108765A1 (en) 2021-06-03
KR20220121816A (ko) 2022-09-01
IL293204A (en) 2022-07-01

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