Nothing Special   »   [go: up one dir, main page]

BR112017017279A2 - métodos para introduzir um gene inibidor de pólen, para introduzir dois genes inibidores de pólen, para introduzir dois genes marcadores coloridos e de introgressão acelerada de traço e planta - Google Patents

métodos para introduzir um gene inibidor de pólen, para introduzir dois genes inibidores de pólen, para introduzir dois genes marcadores coloridos e de introgressão acelerada de traço e planta

Info

Publication number
BR112017017279A2
BR112017017279A2 BR112017017279A BR112017017279A BR112017017279A2 BR 112017017279 A2 BR112017017279 A2 BR 112017017279A2 BR 112017017279 A BR112017017279 A BR 112017017279A BR 112017017279 A BR112017017279 A BR 112017017279A BR 112017017279 A2 BR112017017279 A2 BR 112017017279A2
Authority
BR
Brazil
Prior art keywords
introducing
genes
methods
pollen inhibitor
trait
Prior art date
Application number
BR112017017279A
Other languages
English (en)
Inventor
Ye Huaxun
Daniel Perugini Leandro
T Elsing Michael
James Gordon-Kamm William
Original Assignee
Pioneer Hi Bred Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pioneer Hi Bred Int filed Critical Pioneer Hi Bred Int
Publication of BR112017017279A2 publication Critical patent/BR112017017279A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C12N15/821—Non-antibiotic resistance markers, e.g. morphogenetic, metabolic markers
    • C12N15/8212—Colour markers, e.g. beta-glucoronidase [GUS], green fluorescent protein [GFP], carotenoid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/825—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving pigment biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8287—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
    • C12N15/8289—Male sterility

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Nutrition Science (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)

Abstract

são fornecidos composições e métodos para o uso de genes inibidores de pólen e/ou genes marcadores coloridos na introgressão acelerada de traço. composições e métodos também são fornecidos para a introdução de um gene inibidor de pólen e/ou gene marcador colorido em estreita proximidade com um locus de traço de interesse. métodos de melhoramento e métodos para selecionar plantas que compreendem um locus de traço de interesse em estreita proximidade com pelo menos um gene inibidor de pólen e/ou gene marcador colorido também são divulgados. os métodos e composições empregam pelo menos um gene inibidor de pólen e/ou gene marcador colorido para fornecer um sistema efetivo para introgressão acelerada de traço no genoma de uma planta.
BR112017017279A 2015-03-19 2016-03-16 métodos para introduzir um gene inibidor de pólen, para introduzir dois genes inibidores de pólen, para introduzir dois genes marcadores coloridos e de introgressão acelerada de traço e planta BR112017017279A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562135261P 2015-03-19 2015-03-19
PCT/US2016/022621 WO2016149352A1 (en) 2015-03-19 2016-03-16 Methods and compositions for accelerated trait introgression

Publications (1)

Publication Number Publication Date
BR112017017279A2 true BR112017017279A2 (pt) 2018-04-17

Family

ID=55646891

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112017017279A BR112017017279A2 (pt) 2015-03-19 2016-03-16 métodos para introduzir um gene inibidor de pólen, para introduzir dois genes inibidores de pólen, para introduzir dois genes marcadores coloridos e de introgressão acelerada de traço e planta

Country Status (7)

Country Link
US (2) US11492632B2 (pt)
EP (2) EP3270685B1 (pt)
CN (1) CN107429258A (pt)
AR (1) AR104024A1 (pt)
BR (1) BR112017017279A2 (pt)
CA (1) CA2975279A1 (pt)
WO (1) WO2016149352A1 (pt)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3036327B1 (en) 2013-08-22 2019-05-08 Pioneer Hi-Bred International, Inc. Genome modification using guide polynucleotide/cas endonuclease systems and methods of use
US11236348B2 (en) 2016-11-29 2022-02-01 Pioneer Hi-Bred International, Inc. Transcriptional terminators for gene expression in plants
CR20190260A (es) * 2016-12-02 2019-06-24 Syngenta Participations Ag Edición génica e inducción de haploides simultáneas
KR20200124702A (ko) 2018-02-23 2020-11-03 파이어니어 하이 부렛드 인터내쇼날 인코포레이팃드 신규한 cas9 오르소로그
CA3092071A1 (en) * 2018-03-12 2019-09-19 Pioneer Hi-Bred International, Inc. Methods for plant transformation involving morphogenic gene expression and trait gene integration
CN108220333B (zh) * 2018-03-28 2021-01-05 中国农业科学院作物科学研究所 一种高效植物受体孤雌生殖单倍体筛选方法
CA3117228A1 (en) 2018-12-14 2020-06-18 Pioneer Hi-Bred International, Inc. Novel crispr-cas systems for genome editing
AU2020208190B2 (en) * 2019-01-15 2022-07-28 Seminis Vegetable Seeds, Inc. Green bean plants with improved disease resistance
US11807862B2 (en) 2019-01-15 2023-11-07 Seminis Vegetable Seeds, Inc. Green bean plants with improved disease resistance
CA3123457A1 (en) * 2019-03-11 2020-09-17 Pioneer Hi-Bred International, Inc. Methods for clonal plant production
WO2022165507A1 (en) * 2021-01-28 2022-08-04 Central Coast Agriculture, Inc. Marker-assisted breeding in cannabis plants
WO2023049728A1 (en) * 2021-09-21 2023-03-30 Pairwise Plants Services, Inc. Color-based and/or visual methods for identifying the presence of a transgene and compositions and constructs relating to the same
WO2023247773A1 (en) * 2022-06-24 2023-12-28 Meiogenix Induction of meiotic recombination using a crispr system

Family Cites Families (82)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ201918A (en) 1981-09-18 1987-04-30 Genentech Inc N-terminal methionyl analogues of bovine growth hormone
US5380831A (en) 1986-04-04 1995-01-10 Mycogen Plant Science, Inc. Synthetic insecticidal crystal protein gene
US4945050A (en) 1984-11-13 1990-07-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
US5569597A (en) 1985-05-13 1996-10-29 Ciba Geigy Corp. Methods of inserting viral DNA into plant material
US5268463A (en) 1986-11-11 1993-12-07 Jefferson Richard A Plant promoter α-glucuronidase gene construct
US5608142A (en) 1986-12-03 1997-03-04 Agracetus, Inc. Insecticidal cotton plants
US4873192A (en) 1987-02-17 1989-10-10 The United States Of America As Represented By The Department Of Health And Human Services Process for site specific mutagenesis without phenotypic selection
US5316931A (en) 1988-02-26 1994-05-31 Biosource Genetics Corp. Plant viral vectors having heterologous subgenomic promoters for systemic expression of foreign genes
US5990387A (en) 1988-06-10 1999-11-23 Pioneer Hi-Bred International, Inc. Stable transformation of plant cells
US5023179A (en) 1988-11-14 1991-06-11 Eric Lam Promoter enhancer element for gene expression in plant roots
US5110732A (en) 1989-03-14 1992-05-05 The Rockefeller University Selective gene expression in plants
KR920701453A (ko) 1989-03-17 1992-08-11 미리엄 디. 멕코나헤이 유전자발현의 외부조절
US5034323A (en) 1989-03-30 1991-07-23 Dna Plant Technology Corporation Genetic engineering of novel plant phenotypes
US5231020A (en) 1989-03-30 1993-07-27 Dna Plant Technology Corporation Genetic engineering of novel plant phenotypes
US5879918A (en) 1989-05-12 1999-03-09 Pioneer Hi-Bred International, Inc. Pretreatment of microprojectiles prior to using in a particle gun
US5240855A (en) 1989-05-12 1993-08-31 Pioneer Hi-Bred International, Inc. Particle gun
US5310667A (en) 1989-07-17 1994-05-10 Monsanto Company Glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate synthases
US5322783A (en) 1989-10-17 1994-06-21 Pioneer Hi-Bred International, Inc. Soybean transformation by microparticle bombardment
DE69133128T2 (de) 1990-04-12 2003-06-18 Syngenta Participations Ag, Basel Gewebe-spezifische Promotoren
US5498830A (en) 1990-06-18 1996-03-12 Monsanto Company Decreased oil content in plant seeds
US5633435A (en) 1990-08-31 1997-05-27 Monsanto Company Glyphosate-tolerant 5-enolpyruvylshikimate-3-phosphate synthases
US5866775A (en) 1990-09-28 1999-02-02 Monsanto Company Glyphosate-tolerant 5-enolpyruvyl-3-phosphoshikimate synthases
US5932782A (en) 1990-11-14 1999-08-03 Pioneer Hi-Bred International, Inc. Plant transformation method using agrobacterium species adhered to microprojectiles
US5459252A (en) 1991-01-31 1995-10-17 North Carolina State University Root specific gene promoter
US5399680A (en) 1991-05-22 1995-03-21 The Salk Institute For Biological Studies Rice chitinase promoter
AU668096B2 (en) 1991-08-27 1996-04-26 Syngenta Participations Ag Proteins with insecticidal properties against homopteran insects and their use in plant protection
ZA927576B (en) 1991-10-04 1993-04-16 Univ North Carolina State Pathogen-resistant transgenic plants.
TW261517B (pt) 1991-11-29 1995-11-01 Mitsubishi Shozi Kk
US5324646A (en) 1992-01-06 1994-06-28 Pioneer Hi-Bred International, Inc. Methods of regeneration of Medicago sativa and expressing foreign DNA in same
WO1994001572A2 (en) 1992-07-09 1994-01-20 Pioneer Hi-Bred International, Inc. Maize pollen-specific polygalacturonase gene
US5401836A (en) 1992-07-16 1995-03-28 Pioneer Hi-Bre International, Inc. Brassica regulatory sequence for root-specific or root-abundant gene expression
BR9306802A (pt) 1992-07-27 1998-12-08 Pioneer Hi Bred Int Processo independente de genótipos para produção de planta de soja transgénica e processo de regeneração de plantas de soja a partir de nodos cotiledonais
EP0625006A4 (en) 1992-11-20 1996-04-24 Agracetus Transgenic cotton plants producing heterologous bioplastic.
IL108241A (en) 1992-12-30 2000-08-13 Biosource Genetics Corp Plant expression system comprising a defective tobamovirus replicon integrated into the plant chromosome and a helper virus
WO1994016078A2 (en) 1993-01-13 1994-07-21 Pioneer Hi-Bred International, Inc. High lysine derivatives of alpha-hordothionin
US5527695A (en) 1993-01-29 1996-06-18 Purdue Research Foundation Controlled modification of eukaryotic genomes
US5789156A (en) 1993-06-14 1998-08-04 Basf Ag Tetracycline-regulated transcriptional inhibitors
US5814618A (en) 1993-06-14 1998-09-29 Basf Aktiengesellschaft Methods for regulating gene expression
US5470353A (en) 1993-10-20 1995-11-28 Hollister Incorporated Post-operative thermal blanket
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5837458A (en) 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US5633363A (en) 1994-06-03 1997-05-27 Iowa State University, Research Foundation In Root preferential promoter
US5736369A (en) 1994-07-29 1998-04-07 Pioneer Hi-Bred International, Inc. Method for producing transgenic cereal plants
US5792931A (en) 1994-08-12 1998-08-11 Pioneer Hi-Bred International, Inc. Fumonisin detoxification compositions and methods
US5608144A (en) 1994-08-12 1997-03-04 Dna Plant Technology Corp. Plant group 2 promoters and uses thereof
US5750868A (en) 1994-12-08 1998-05-12 Pioneer Hi-Bred International, Inc. Reversible nuclear genetic system for male sterility in transgenic plants
CA2222600A1 (en) 1995-06-02 1996-12-05 Pioneer Hi-Bred International, Inc. High methionine derivatives of .alpha.-hordothionin
BR9609200A (pt) 1995-06-02 1999-05-11 Pioneer Hi Bred Int Derivados de alta treonina de alpha-hordotionina
US5962769A (en) 1995-06-07 1999-10-05 Pioneer Hi-Bred International, Inc. Induction of male sterility in plants by expression of high levels of avidin
US5837876A (en) 1995-07-28 1998-11-17 North Carolina State University Root cortex specific gene promoter
US5703049A (en) 1996-02-29 1997-12-30 Pioneer Hi-Bred Int'l, Inc. High methionine derivatives of α-hordothionin for pathogen-control
US5850016A (en) 1996-03-20 1998-12-15 Pioneer Hi-Bred International, Inc. Alteration of amino acid compositions in seeds
US6072050A (en) 1996-06-11 2000-06-06 Pioneer Hi-Bred International, Inc. Synthetic promoters
CA2270289C (en) 1996-11-01 2005-09-27 Pioneer Hi-Bred International, Inc. Proteins with enhanced levels of essential amino acids
US5981840A (en) 1997-01-24 1999-11-09 Pioneer Hi-Bred International, Inc. Methods for agrobacterium-mediated transformation
ATE317440T1 (de) 1997-11-18 2006-02-15 Pioneer Hi Bred Int Neuartige methode zur integration von fremd-dna ins eukaryotische genom
ES2308327T3 (es) 1997-11-18 2008-12-01 Pioneer Hi-Bred International, Inc. Composiciones y metodos para modificacion genetica de plantas.
US5929301A (en) 1997-11-18 1999-07-27 Pioneer Hi-Bred International Nucleic acid sequence encoding FLP recombinase
WO1999025855A1 (en) 1997-11-18 1999-05-27 Pioneer Hi-Bred International, Inc. Mobilization of viral genomes from t-dna using site-specific recombination systems
US6177611B1 (en) 1998-02-26 2001-01-23 Pioneer Hi-Bred International, Inc. Maize promoters
EP1104469B1 (en) 1998-08-20 2005-11-09 Pioneer Hi-Bred International, Inc. Seed-preferred promoters
WO2000012733A1 (en) 1998-08-28 2000-03-09 Pioneer Hi-Bred International, Inc. Seed-preferred promoters from end genes
IL142736A0 (en) 1998-11-09 2002-03-10 Pioneer Hi Bred Int Transcriptional activator lec 1 nucleic acids, polypeptides and their uses
IL146062A0 (en) 1999-04-29 2002-07-25 Marlow Foods Ltd Herbicide resistant plants
WO2001000158A1 (en) 1999-06-28 2001-01-04 The Procter & Gamble Company Cosmetic compositions containing quaternary ammonium compounds
US6683231B2 (en) 2000-06-02 2004-01-27 E. I. Du Pont De Nemours And Company High level production of p-hydroxybenzoic acid in green plants
CN100558897C (zh) 2000-09-29 2009-11-11 辛根塔有限公司 抗除草剂植物
US7045684B1 (en) 2002-08-19 2006-05-16 Mertec, Llc Glyphosate-resistant plants
EP1907553B1 (en) 2005-07-18 2012-08-22 Pioneer Hi-Bred International Inc. Modified frt recombination sites and methods of use
WO2008021974A1 (en) 2006-08-11 2008-02-21 Monsanto Technology Llc Production of high tryptophan maize by chloroplast targeted expression of anthranilate synthase
EP2568048A1 (en) * 2007-06-29 2013-03-13 Pioneer Hi-Bred International, Inc. Methods for altering the genome of a monocot plant cell
MX2010002304A (es) * 2007-08-29 2010-03-18 Monsanto Technology Llc Metodos para incorporar multiples genes en una planta de cultivo.
EP2206723A1 (en) 2009-01-12 2010-07-14 Bonas, Ulla Modular DNA-binding domains
JP5208842B2 (ja) 2009-04-20 2013-06-12 株式会社カプコン ゲームシステム、ゲーム制御方法、プログラム、および、このプログラムを記録したコンピュータ読み取り可能な記録媒体
US8481811B2 (en) 2009-04-30 2013-07-09 Pioneer Hi-Bred International, Inc. Maize cystatin promoter for pericarp preferred expression
BR112012004282A2 (pt) 2009-08-25 2015-09-01 Targeted Growth Inc Transgene modificado codificando uma proteína de crescimento ou relacionada a desenvolvimento em plantas
US20110203012A1 (en) * 2010-01-21 2011-08-18 Dotson Stanton B Methods and compositions for use of directed recombination in plant breeding
US20130055791A1 (en) 2010-02-12 2013-03-07 Gl Sciences Incorporated Sample trapping method and sample trapping apparatus
BR112014002622A2 (pt) 2011-08-03 2019-09-24 Du Pont método para introduzir no genoma de uma célula vegetal um sítio alvo para a integração específica de sítio, célula vegetal, parte de planta, planta ou semente, método para integrar um polinucleotídeo de interesse em um sítio alvo no genoma de uma célula vegetal
AR089793A1 (es) * 2012-01-27 2014-09-17 Du Pont Metodos y composiciones para generar locus de rasgos transgenicos complejos
US11039586B2 (en) * 2013-03-15 2021-06-22 Monsanto Technology Llc Creation and transmission of megaloci
EP3036327B1 (en) * 2013-08-22 2019-05-08 Pioneer Hi-Bred International, Inc. Genome modification using guide polynucleotide/cas endonuclease systems and methods of use

Also Published As

Publication number Publication date
EP3270685B1 (en) 2023-07-19
CN107429258A (zh) 2017-12-01
WO2016149352A1 (en) 2016-09-22
US20230020758A1 (en) 2023-01-19
AR104024A1 (es) 2017-06-21
US20180119162A1 (en) 2018-05-03
CA2975279A1 (en) 2016-09-22
EP3270685A1 (en) 2018-01-24
US11492632B2 (en) 2022-11-08
EP4118955A1 (en) 2023-01-18

Similar Documents

Publication Publication Date Title
BR112017017279A2 (pt) métodos para introduzir um gene inibidor de pólen, para introduzir dois genes inibidores de pólen, para introduzir dois genes marcadores coloridos e de introgressão acelerada de traço e planta
BR112017018327A2 (pt) método para a expressão regulada de um complexo, planta ou célula vegetal, semente, método para modificação de uma sequência, método para alteração da expressão de pelo menos um polinucleotídeo
BR112018076027A2 (pt) método para modificar uma sequência-alvo no genoma de uma célula vegetal; método para editar uma sequência de nucleotídeos no genoma de uma célula vegetal; método para modificar simultaneamente múltiplas sequências-alvo no genoma de uma célula vegetal; método para modificar uma sequênciaalvo de dna no genoma de uma célula vegetal e modelo de modificação de polinucleotídeo
BR112019023377A2 (pt) Criação de um gene resistente a herbicida e uso do mesmo
BR112017000621A2 (pt) método para melhorar um traço agronômico de uma planta, método para melhorar a produtividade de grãos ou a eficiência de uso de nitrogênio de uma planta de milho ou de cultura, método para editar, substituir ou eliminar uma sequência reguladora, método para inserir um promotor ou elemento do promotor no genoma de uma célula vegetal e método para fornecer um perfil de expressão adicional para um polinucleotídeo endógeno de uma célula vegetal
BR112018008134A2 (pt) método para restaurar a função de um produto gênico não funcional no genoma de uma célula, método para editar uma sequência nucleotídica no genoma de uma célula, planta ou planta de progênie, método para editar uma sequência nucleotídica no genoma de uma célula sem o uso de um molde polinucleotídico de modificação e método para entregar um complexo de rna guia/endonuclease cas numa célula
AR110075A1 (es) Un método para edición basal en plantas
BR112017012851A2 (pt) sistemas de modificação de genoma fúngico e métodos de uso
BR112018075099A2 (pt) novos loci genéticos associados a resistência a doença em soja
BR112013024337A2 (pt) locus de traço transgênico complexo em uma planta, planta ou semente, método para produzir em uma planta um locus de traço transgênico complexo e construto de expressão
BR112018010341A2 (pt) composição, sistema e método para tratamento do cabelo, método para alteração da cor do cabelo, e, kit de multicompartimentos para alteração da cor ou do formato do cabelo.
BR112017024429A2 (pt) polinucleotídeo isolado, construto de dna, método para conferir resistência a doenças, planta, semente, método para reduzir um ou mais sintomas de uma doença, método para produzir uma planta, método para testar uma planta, método para melhorar a resistência de plantas
BR112016026136A2 (pt) composições e métodos para aumentar o desenvolvimento e o rendimento vegetal
BR112022001543A2 (pt) Loci genéticos associados à resistência a doenças na soja
CL2019001742A1 (es) Evento de élite ee-gm5 y métodos y kits para identificar dicho evento en muestras biológicas.
BR112019005103A2 (pt) método para determinar a quantidade requerida por plantas de cultura em um campo, método para tratar plantas de cultura em um campo, sistema e produto de programa de computador
BR112018005629A2 (pt) promotor de planta e 3' utr para expressão de transgene
BR112015022778A2 (pt) composição de óleo de soja modificado através do silenciamento direcionado do(s) gene(s) fad2-1a/1b seu método de produção e uso de uma planta de soja, parte de planta, célula de planta, ou semente de soja com o(s) gene(s) fad2-1a/1b silenciado(s)
CO2019007826A2 (es) Evento de élite ee-gm4 y métodos y kits para identificar dicho evento en muestras biológicas
BR112016019007A2 (pt) locais genéticos associados a fertilidade acrescida em milho
BR112016014568A2 (pt) método para identificar e/ou selecionar uma planta, método de introgressão de um alelo de locus, método para identificar uma planta
BR112018000541A2 (pt) métodos para produção de plantas de algodão com tolerância à seca melhorada e composições dos mesmos.
BR112015022555A2 (pt) métodos de detecção de uma primeira planta, de melhoramento de plantas de soja e para prolongar a duração de um estágio de desenvolvimento reprodutivo
BR112016021044A2 (pt) método de detecção de um perfil do marcador
BR112018006573A2 (pt) método para a produção de plantas haploides e subsequentes haploides duplicadas

Legal Events

Date Code Title Description
B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B11B Dismissal acc. art. 36, par 1 of ipl - no reply within 90 days to fullfil the necessary requirements