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BR112014012773A2 - ensaio de polimorfismo de nucleotídeo único de alto rendimento - Google Patents

ensaio de polimorfismo de nucleotídeo único de alto rendimento

Info

Publication number
BR112014012773A2
BR112014012773A2 BR112014012773A BR112014012773A BR112014012773A2 BR 112014012773 A2 BR112014012773 A2 BR 112014012773A2 BR 112014012773 A BR112014012773 A BR 112014012773A BR 112014012773 A BR112014012773 A BR 112014012773A BR 112014012773 A2 BR112014012773 A2 BR 112014012773A2
Authority
BR
Brazil
Prior art keywords
single nucleotide
nucleotide polymorphism
high yield
assay
yield single
Prior art date
Application number
BR112014012773A
Other languages
English (en)
Inventor
Todd Gerdes James
Martin Benson Robert
P Kumpatla Siva
Gao Wenxiang
Original Assignee
Agrigenetics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agrigenetics Inc filed Critical Agrigenetics Inc
Publication of BR112014012773A2 publication Critical patent/BR112014012773A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

resumo patente de invenção: "ensaio de polimorfismo de nucleotídeo único de alto rendimento". a presente invenção refere-se a um método que consiste em um sistema de detecção de ensaio homogêneo para um processo de pcr utilizando fret para a detecção e análise da zigosidade do polimorfismo de um nucleotídeo único haahasl1-a122 (at) t em girassol é fornecido. o método proporciona primers do genoma do girassol específicos que podem ser usados para detectar a presença ou a ausência do polimorfismo de nucleotídeo único haahasl1-a122 (at) t. as combinações de primers para uso em um ensaio de pcr de endpoint capaz de determinar a zigosidade e para auxiliar na introgressão são descritas.
BR112014012773A 2011-11-29 2012-11-27 ensaio de polimorfismo de nucleotídeo único de alto rendimento BR112014012773A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161564464P 2011-11-29 2011-11-29
PCT/US2012/066577 WO2013081987A1 (en) 2011-11-29 2012-11-27 High throughput single nucleotide polymorphism assay

Publications (1)

Publication Number Publication Date
BR112014012773A2 true BR112014012773A2 (pt) 2019-09-24

Family

ID=48467207

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112014012773A BR112014012773A2 (pt) 2011-11-29 2012-11-27 ensaio de polimorfismo de nucleotídeo único de alto rendimento

Country Status (10)

Country Link
US (1) US20130137097A1 (pt)
EP (1) EP2786146A4 (pt)
CN (1) CN103988079A (pt)
AR (1) AR088998A1 (pt)
BR (1) BR112014012773A2 (pt)
CA (1) CA2854790A1 (pt)
CL (1) CL2014001406A1 (pt)
RU (1) RU2014126423A (pt)
UY (1) UY34473A (pt)
WO (1) WO2013081987A1 (pt)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11001881B2 (en) 2006-08-24 2021-05-11 California Institute Of Technology Methods for detecting analytes
US8048626B2 (en) 2006-07-28 2011-11-01 California Institute Of Technology Multiplex Q-PCR arrays
US11525156B2 (en) 2006-07-28 2022-12-13 California Institute Of Technology Multiplex Q-PCR arrays
US11560588B2 (en) 2006-08-24 2023-01-24 California Institute Of Technology Multiplex Q-PCR arrays
US9708647B2 (en) 2015-03-23 2017-07-18 Insilixa, Inc. Multiplexed analysis of nucleic acid hybridization thermodynamics using integrated arrays
US9499861B1 (en) 2015-09-10 2016-11-22 Insilixa, Inc. Methods and systems for multiplex quantitative nucleic acid amplification
WO2017155858A1 (en) 2016-03-07 2017-09-14 Insilixa, Inc. Nucleic acid sequence identification using solid-phase cyclic single base extension
EP3937780A4 (en) 2019-03-14 2022-12-07 InSilixa, Inc. METHODS AND SYSTEMS FOR TIMED FLUORESCENCE-BASED DETECTION
CN116287373B (zh) * 2022-12-31 2024-07-09 中国农业大学 小麦穗粒数主效qtl紧密连锁的kasp分子标记及其方法与应用

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110014706A2 (en) * 1998-12-14 2011-01-20 Monsanto Technology Llc Arabidopsis thaliana Genome Sequence and Uses Thereof
JP2003325200A (ja) * 2002-03-08 2003-11-18 Kazuko Matsumoto 新規高感度核酸解析法
AU2006265984C9 (en) * 2005-07-01 2013-04-11 Basf Agrochemical Products B.V. Herbicide-resistant sunflower plants, polynucleotides encoding herbicide-resistant acetohydroxyacid synthase large subunit proteins, and methods of use
EP1956097A1 (en) * 2007-02-06 2008-08-13 bioMerieux B.V. Method for discriminating single nucleotide polymorphisms (SNPs)
HUE032487T2 (en) * 2007-04-04 2017-09-28 Basf Se Herbicide-resistant brassica plants and application procedures
AU2008237436B2 (en) * 2007-04-04 2014-01-23 Basf Agrochemical Products B.V. Herbicide-resistant sunflower plants with multiple herbicide resistant alleles of AHASL1 and methods of use
GB0719775D0 (en) * 2007-10-10 2007-11-21 Isis Innovation Extended haplotypes
CN101220395A (zh) * 2008-02-03 2008-07-16 中国科学院化学研究所 一种检测dna单核苷酸多态性的方法
UA115019C2 (uk) * 2008-09-26 2017-09-11 Басф Агрокемікал Продактс Б.В. Стійкий до гербіцидів ahas-мутант рослини brassica
CN102191314A (zh) * 2010-03-12 2011-09-21 孙朝辉 一种基于pcr的低成本、简单、高效的snp检测方法

Also Published As

Publication number Publication date
AR088998A1 (es) 2014-07-23
CL2014001406A1 (es) 2015-01-16
CN103988079A (zh) 2014-08-13
EP2786146A1 (en) 2014-10-08
EP2786146A4 (en) 2015-08-26
WO2013081987A1 (en) 2013-06-06
UY34473A (es) 2013-06-28
RU2014126423A (ru) 2016-01-27
CA2854790A1 (en) 2013-06-06
US20130137097A1 (en) 2013-05-30

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Legal Events

Date Code Title Description
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]
B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: EM VIRTUDE DO ARQUIVAMENTO PUBLICADO NA RPI 2543 DE 01-10-2019 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDO O ARQUIVAMENTO DO PEDIDO DE PATENTE, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013.