BR0005045A - Método para sexagem de espermatozóides usando a via clássica do sistema complemento, com eliminação da via alternativa pela inativação da proteina "b" - Google Patents
Método para sexagem de espermatozóides usando a via clássica do sistema complemento, com eliminação da via alternativa pela inativação da proteina "b"Info
- Publication number
- BR0005045A BR0005045A BR0005045-8A BR0005045A BR0005045A BR 0005045 A BR0005045 A BR 0005045A BR 0005045 A BR0005045 A BR 0005045A BR 0005045 A BR0005045 A BR 0005045A
- Authority
- BR
- Brazil
- Prior art keywords
- pathway
- sperm
- complement system
- complement
- protein
- Prior art date
Links
- 230000037361 pathway Effects 0.000 title abstract 7
- 102000004169 proteins and genes Human genes 0.000 title abstract 6
- 108090000623 proteins and genes Proteins 0.000 title abstract 6
- 230000004154 complement system Effects 0.000 title abstract 4
- 238000000034 method Methods 0.000 title abstract 4
- 230000000415 inactivating effect Effects 0.000 title 1
- 230000000295 complement effect Effects 0.000 abstract 2
- 210000002966 serum Anatomy 0.000 abstract 2
- 241000283690 Bos taurus Species 0.000 abstract 1
- 241000700199 Cavia porcellus Species 0.000 abstract 1
- 108010042653 IgA receptor Proteins 0.000 abstract 1
- 102100034014 Prolyl 3-hydroxylase 3 Human genes 0.000 abstract 1
- 230000003213 activating effect Effects 0.000 abstract 1
- 230000006037 cell lysis Effects 0.000 abstract 1
- 238000005138 cryopreservation Methods 0.000 abstract 1
- 230000009089 cytolysis Effects 0.000 abstract 1
- 230000008030 elimination Effects 0.000 abstract 1
- 238000003379 elimination reaction Methods 0.000 abstract 1
- 230000004720 fertilization Effects 0.000 abstract 1
- 238000010438 heat treatment Methods 0.000 abstract 1
- 238000000338 in vitro Methods 0.000 abstract 1
- 230000002779 inactivation Effects 0.000 abstract 1
- 230000009027 insemination Effects 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 239000003550 marker Substances 0.000 abstract 1
- 210000000582 semen Anatomy 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5091—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing the pathological state of an organism
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/46—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from vertebrates
- G01N2333/47—Assays involving proteins of known structure or function as defined in the subgroups
- G01N2333/4701—Details
- G01N2333/4716—Complement proteins, e.g. anaphylatoxin, C3a, C5a
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Urology & Nephrology (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- Medicinal Chemistry (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Physiology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Food Science & Technology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
"MéTODO PARA SEXAGEM DE ESPERMATOZóIDES USANDO A VIA CLáSSICA DO SISTEMA COMPLEMENTO, COM ELIMINAçãO DA VIA ALTERNATIVA PELA INATIVAçãO DA PROTEINA "B". A produção de metodologia para realizar a imunossexagem de espermatozóides bovinos utilizando-se anticorpos monoclonais associados a proteínas da via clássica do sistema complemento é o objetivo desta patente. Anticorpos monoclonais direcionados contra proteínas sexo-específicas associados ao complemento provoca a lise celular por proteínas da via clássica do sistema complemento. Para que esta metodologia possa ser utilizada é necessário a eliminação da via alternativa do complemento, o que é a base da nossa tecnologia, onde eliminamos a ação da proteína B, responsável pela ativação da via alternativa, pelo aquecimento do soro de cobaio à 52N<0>C<sym>0,3 por 30 minutos. Os espermatozóides são colocados em contato com o anticorpo monoclonal específico contra a proteína marcadora da característica sexual. Em seguida, adiciona-se soro aquecido de forma apenas a via clássica do sistema complemento é ativada para que promova a lise específica dos espermatozóides marcados. Assim, os espermatozóides do sêmen tratado estarão prontos para serem processados conforme a finalidade, inseminação, criopreservação ou fertilização "in vitro".
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| BR0005045-8A BR0005045A (pt) | 2000-09-28 | 2000-09-28 | Método para sexagem de espermatozóides usando a via clássica do sistema complemento, com eliminação da via alternativa pela inativação da proteina "b" |
| US09/965,017 US20020115055A1 (en) | 2000-09-28 | 2001-09-27 | Gender differentiation of bovine sperm cells |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| BR0005045-8A BR0005045A (pt) | 2000-09-28 | 2000-09-28 | Método para sexagem de espermatozóides usando a via clássica do sistema complemento, com eliminação da via alternativa pela inativação da proteina "b" |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR0005045A true BR0005045A (pt) | 2002-05-14 |
Family
ID=3945623
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR0005045-8A BR0005045A (pt) | 2000-09-28 | 2000-09-28 | Método para sexagem de espermatozóides usando a via clássica do sistema complemento, com eliminação da via alternativa pela inativação da proteina "b" |
Country Status (2)
| Country | Link |
|---|---|
| US (1) | US20020115055A1 (pt) |
| BR (1) | BR0005045A (pt) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1017987B1 (en) | 1997-01-31 | 2005-06-15 | The Horticulture And Food Research Institute Of New Zealand Limited | Optical apparatus and method |
| US6149867A (en) | 1997-12-31 | 2000-11-21 | Xy, Inc. | Sheath fluids and collection systems for sex-specific cytometer sorting of sperm |
| US7208265B1 (en) | 1999-11-24 | 2007-04-24 | Xy, Inc. | Method of cryopreserving selected sperm cells |
| US7713687B2 (en) | 2000-11-29 | 2010-05-11 | Xy, Inc. | System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations |
| WO2002043574A2 (en) | 2000-11-29 | 2002-06-06 | Xy, Inc. | System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations |
| EP1545203B1 (en) | 2002-08-01 | 2016-10-19 | Xy, Llc | Low pressure sperm cell separation system |
| US8486618B2 (en) | 2002-08-01 | 2013-07-16 | Xy, Llc | Heterogeneous inseminate system |
| BRPI0313476B1 (pt) | 2002-08-15 | 2015-06-23 | Xy Llc | Citômetro de fluxo de alta resolução |
| US7169548B2 (en) | 2002-09-13 | 2007-01-30 | Xy, Inc. | Sperm cell processing and preservation systems |
| US8271279B2 (en) * | 2003-02-21 | 2012-09-18 | Qnx Software Systems Limited | Signature noise removal |
| EP2308418B1 (en) | 2003-03-28 | 2015-12-02 | Inguran, LLC | Flow cytometer nozzle for providing sex-sorted animal sperm |
| CA2566749C (en) | 2003-05-15 | 2017-02-21 | Xy, Inc. | Efficient haploid cell sorting for flow cytometer systems |
| AU2005228893B2 (en) | 2004-03-29 | 2010-08-19 | Inguran, Llc | Sperm suspensions for sorting into X or Y chromosome-bearing enriched populations |
| BRPI0401765C1 (pt) * | 2004-05-17 | 2011-03-15 | Hy Biotecnologica Ltda | método de enriquecimento de espermatozoides de mamìferos portadores de cromossomo x ou de cromossomo y |
| EP2269617B1 (en) | 2004-07-22 | 2016-04-27 | Inguran, LLC | Process for enriching a population of sperm cells |
| US8771934B2 (en) | 2011-12-09 | 2014-07-08 | The Curators Of The University Of Missouri | Inorganic pyrophosphate and uses thereof |
| KR101916959B1 (ko) * | 2015-07-13 | 2018-11-08 | 김동구 | 정자의 성 감별용 항체 및 이의 용도 |
-
2000
- 2000-09-28 BR BR0005045-8A patent/BR0005045A/pt not_active IP Right Cessation
-
2001
- 2001-09-27 US US09/965,017 patent/US20020115055A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| US20020115055A1 (en) | 2002-08-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| BR0005045A (pt) | Método para sexagem de espermatozóides usando a via clássica do sistema complemento, com eliminação da via alternativa pela inativação da proteina "b" | |
| Coy et al. | Oviduct-specific glycoprotein and heparin modulate sperm–zona pellucida interaction during fertilization and contribute to the control of polyspermy | |
| Arangasamy et al. | Isolation and characterization of heparin and gelatin binding buffalo seminal plasma proteins and their effect on cauda epididymal spermatozoa | |
| Kouba et al. | Effects of the porcine oviduct-specific glycoprotein on fertilization, polyspermy, and embryonic development in vitro | |
| AR026572A1 (es) | Metodo de celula de esperma seleccionadas por criopreservacion | |
| Do et al. | Melatonin supplementation during in vitro maturation and development supports the development of porcine embryos | |
| Ratto et al. | Ovulation-inducing factor: a protein component of llama seminal plasma | |
| Singh et al. | Adhesion of Tritrichomonas foetus to bovine vaginal epithelial cells | |
| BRPI0507174A (pt) | proteìnas de fusão hormÈnio-fc (fsh-fc) heterodiméricas estimuladoras de folìculo para o tratamento da infertilidade | |
| PE20010694A1 (es) | Plasmido autonomamente reproducible en bacterias corineformes | |
| Latorraca et al. | Autophagy is a pro-survival adaptive response to heat shock in bovine cumulus-oocyte complexes | |
| CO2023006034A2 (es) | Sistema para transferencia de embriones | |
| Ducummon et al. | Localization of the Rho GTPases and some Rho effector proteins in the sperm of several mammalian species | |
| Roberts et al. | Identification of human sperm-coating antigen | |
| Mayer et al. | Localization of the sheep FcRn in the mammary gland | |
| AR042968A1 (es) | Anticuerpos de virus anti-dengue composiciones metodos y usos | |
| Dunbar et al. | Inhibition of fertilization in vitro by treatment of rabbit spermatozoa with univalent isoantibodies to rabbit sperm hyaluronidase | |
| Crispo et al. | Rapid Communication: Nerve growth factor influences cleavage rate and embryo development in sheep | |
| BRPI0506549A (pt) | método para avaliação da integridade da cromatina/dna e de esperma animal e kit para a avaliação da qualidade do esperma animal | |
| Zannoni et al. | Relative abundance of heat shock proteins and clusterin transcripts in spermatozoa collected from boar routinely utilised in an artificial insemination centre: preliminary results | |
| HN1998000141A (es) | Composicion farmaceutica antimastitica a base de extractos de origen natural y procedimientom para fabricarla. | |
| Shuang-Lin et al. | Antithrombin III enhances the motility and chemotaxis of boar sperm | |
| McBride et al. | Evidence for the regulation of glycosylation of golden hamster (Mesocricetus auratus) oviductin during the estrous cycle | |
| Donohue et al. | Male engorgement factor: Role in stimulating engorgement to repletion in the ixodid tick, Dermacentor variabilis | |
| Harikrishna et al. | Purification of regucalcin from the seminal vesicular fluid: a calcium binding multi-functional protein |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B08F | Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette] |
Free format text: REFERENTE A 6A , 7A , 8A , 9A ,10A E 11A ANUI DADES. |
|
| B08K | Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette] |
Free format text: REFERENTE AO DESPACHO 8.6 PUBLICADO NA RPI 2138 DE 27/12/2011. |