AU734086B2 - Nucleic acid sensor - Google Patents
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- AU734086B2 AU734086B2 AU27578/97A AU2757897A AU734086B2 AU 734086 B2 AU734086 B2 AU 734086B2 AU 27578/97 A AU27578/97 A AU 27578/97A AU 2757897 A AU2757897 A AU 2757897A AU 734086 B2 AU734086 B2 AU 734086B2
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Description
I, WO 97/44651 PCT/AU97/00316 1 Nucleic Acid Sensor FIELD OF INVENTION The present invention relates to a biosensor for use in detecting the presence of a selected nucleic acid sequence in a sample and to a method of detecting the presence of a selected nucleic acid (NA) sequence in a sample.
BACKGROUND OF THE INVENTION Assays for the presence of specific DNA or RNA sequences in samples have applications in many fields. For example, infection of patients with a particular microorganism can be assessed by analyzing a biological sample from the patient for the presence of a NA sequence specific for the microorganism. Other applications include the analysis of food or environmental samples to detect contamination. The detection of genetic disease caused by mutations can also be achieved by these techniques.
To date, the widespread use of this highly sensitive method has been restricted by the need for significant amplification of the amount of the specific nucleic acid sequence and/or the need to use specialized techniques to analyze the amplified DNA This amplification is generally achieved using polymerase chain or ligase chain reaction and the amplified DNA is then separated on a gel and the gel analyzed for the presence of specific bands of
DNA.
Other methods include US 4,840,893, which teaches a method of nucleic acid detection. The method uses a probe sequence with attached ligand, an enzyme mediator system linked to a second ligand and which is capable of transferring a charge to an electrical surface when the enzyme is catalytically active, and an antiligand, to which the first and second ligands compete for binding. The assay is a competition system whereby the binding of the target sequence in a sample affects the availability of the first ligand and alters the rate of charge transfer to the electrode. The present invention has the advantage of providing a direct gating mechanism, which does not rely on a signal generated by enzyme catalysis.
U.S. 4,868,104 describes a method of detecting a nucleotide sequence by the use of two polynucleotide reagents, which bind to different sequences on the target analyte, the first of which can be polymerized and the second has a detection system. Several other patents describe nucleic acid detection methods, most of which involve labelling of the probes. These SUBSTITUTE SHEET (RULE 26) WO 97/44651 PCT/AU97/00316 2 include: U.S. 4,968,602; U.S. 5,116,733; U.S. 4,868,105; U.S. 4,716,106; U.S. 4,883,750; U.S. 5,242,794; U.S. 4,996,142; U.S. 5,348,855 and U.S.
5,053,326.
BRIEF DESCRIPTION OF DRAWINGS Figures 1 to 3 show the results obtained in Examples 1 to 3.
Fig. 4 shows linker gramicidin B Fig. 5 shows membrane spanning lipid Fig. 6 shows linker lipid A Fig. 7 shows biotinylated gramicidin E DESCRIPTION OF THE INVENTION The present inventors propose an apparatus which can be used to detect the presence of a specific NA sequence in a sample. The apparatus is best described as a biosensor in that it involves the use of lipid membranes.
Membranes for the use in biosensors have been disclosed In international Patent Application Nos PCT/AU88/00273, PCT/AU89/00352 PCT/AU90/00025 and PCT/AU92/00132. The disclosure of these applications is included herein by reference.
As disclosed in these applications, suitably modified lipid molecules may be caused to assemble into an electrode/ionic reservoir/insulating bilayer combination that is suitable for incorporation of ion channels and ionophores. It is also disclosed that the conductance of these membranes is dependent on the presence or absence of an analyte. In bilayer membranes in which each layer includes ion channel monomers, the conductance of the membrane is dependent on the lining up of the monomers in each layer to form continuous ion channels which span the membrane. As these continuous ion channels are constantly being formed and destroyed, the conductance of the membrane is dependent on the lifetimes of these continuous ion channels.
In a first aspect, the present invention consists in a biosensor for use in detecting the presence of a selected nucleic acid sequence in a sample, the biosensor comprising an electrode and a bilayer membrane having a top and a bottom layer, the bottom layer being proximal to and connected to the electrode in a manner such that a space exists between the membrane and the electrode, the conductance or impedance of the membrane being dependent on the presence or absence of the selected nucleic acid sequence, WO 97/44651 PCT/AU97/00316 3 the membrane comprising a closely packed array of amphiphilic molecules and a plurality of ion channels comprising first half membrane spanning monomers dispersed in the top layer and second half membrane spanning monomers dispersed in the bottom layer, the first half membrane spanning monomers being capable of lateral diffusion within the upper layer and the second half membrane-spanning monomers being prevented from lateral diffusion within the bottom layer, a first ligand specifically reactive with the selected nucleic acid sequence attached to an end of a proportion of the first half membrane-spanning monomers proximal the surface of the membrane and a second ligand reactive with the selected nucleic acid or marker attached thereto is attached to an end of the remainder of the first half membrane-spanning monomers proximal the surface of the membrane.
In a preferred embodiment of the present invention the membrane includes membrane spanning amphiphiles which are prevented from lateral diffusion within the membrane.
In a second aspect, the present invention consists in a biosensor for use in detecting the presence of a selected nucleic add sequence in a sample, the biosensor comprising an electrode and a bilayer membrane having a top and a bottom layer, the bottom layer being proximal to and connected to the electrode in a manner such that a space exists between the membrane and the electrode, the conductance or impedance of the membrane being dependent on the presence or absence of the selected nucleic acid sequence, the membrane comprising a closely packed array of amphiphilic molecules, membrane spanning amphiphiles which are prevented from lateral diffusion within the membrane and a plurality of ion channels comprising first half membrane spanning monomers dispersed in the top layer and second half membrane spanning monomers dispersed in the bottom layer, the first half membrane spanning monomers being capable of lateral diffusion within the upper layer and the second half membrane-spanning monomers being prevented from lateral diffusion within the bottom layer, a first ligand specifically reactive with the selected nucleic acid sequence attached to either an end of at least a proportion of the first half membrane-spanning monomers proximal the surface of the membrane or to an end of the membrane-spanning amphiphiles proximal the surface of the membrane and a second ligand reactive with the selected nucleic acid or marker attached thereto attached to the other of the at least a proportion of the first half WO 97/44651 PCT/AU97/00316 4 membrane-spanning monomers proximal the surface of the membrane or the end of the membrane-spanning amphiphiles proximal the surface of the membrane.
In a third aspect the present invention consists in a method of detecting the presence of a selected nucleic acid sequence within a sample, the method comprising adding the sample to the biosensor of the first or second aspect of the present invention and detecting change in the impedance or conductance of the membrane.
As will be understood by those of moderate skill in the art the biosensor and method of the present invention functions by the binding of the selected nucleic acid sequence to the first and second ligands causing a change in the ability of ions to traverse the membrane via the ion channels.
The specificity of the detection is provided by the specificity of the first ligand for the selected nucleic acid squence. This specifity may be provided in a number of ways, however, it is presently preferred that this is achieved by the use of first ligand which comprises a nucleic acid molecule or PNA which includes a sequence complementary to a first sequence within the selected nucleic acid. As will be understood the sequence within the first ligand may be of any length sufficient to provide the required level of sensitivity, typically at least 10 residues. It will also be understood the nucleic acid molecule may include modified bases.
As the specificity is provided primarily by the first ligand it is not essential that the second ligand is specific for the selected nucleic acid sequence, it is simply necessary that the second ligand binds nucleic acid or a marker attached thereto. Accordingly, the second ligand may be an antibody directed against DNA, or binding fragment thereof. The second ligand may also be an intercalating agent, major or minor groove binder or an agent capable of triple helix formation or an agent capable of covalently linking to nucleic acids upon activation or combinations thereof. It is preferred that the second ligand is not specific for the selected nucleic acid sequence as this results in simpler assembly of the biosensor as only one specific ligand need be produced for each selected nucleic acid sequence that is to be detected.
The biosensor may also include a variety of second ligands which vary over the biosensor. For example the second ligand attached to one membrane spanning amphiphile may be a different moiety to the second WO 97/44651 PCT/AU97/00316 ligand attached to another membrane spanning amphiphile. The essential feature is that the second ligand binds to nucleic acid or to a marker attached thereto. Accordingly, it is to be understood that the term "second ligand" embraces a single or multiple species of moieties which bind nucleic acid or to a marker attached thereto.
As will appreciated by those skilled in the field in a number of applications the nucleic acid sequence to be detected will be labelled with a marker, for example a biotinylated PCR product.
It is, however, also clearly within the ambit of the present invention that both the first and second ligands are specific for the selected nucleic acid sequence. In such an embodiment the first and second ligands are specific for different sequences within the selected nucleic acid sequence.
The first and second ligands may be attached in any of a number of ways. For example, streptavidin may be attached as described in the applications setout above. This can then be used to bind biotinylated nucleic acid. It is preferred that the first and second ligands are attached via linkers to the ion channels or membrane spanning lipids. Where a linker is used it is preferred that the linker is hydrophilic. It is further preferred that the linker includes a phosphoramidite group.
It is also expected that changing the linker length may affect the gating response detected in the biosensor.
Where the first and second ligands are nucleic acid sequences this may be advantageously achieved by producing a molecule having the two DNA sequences which are to act as the ligands at each end with an RNA sequence in the middle. The two ends of the molecule are then attached to the biosensor surface and the central RNA portion digested with RNaseH to leave the biosensor with the two separated DNA sequences attached.
In a preferred embodiment the first ligand comprises a nucleic acid molecule which includes a sequence complementary to a first sequence within the selected nucleic acid. It is further preferred that the second ligand comprises a nucleic acid molecule which includes a sequence complementary to a second sequence within the selected nucleic acid. Due to the presence of such complementary sequences the first and second ligands bind to the selected nucleic acid by hydridisation.
It is presently preferred that the first and second ligands are oligonucleotide sequences selected to bind to different regions of the selected WO 97/44651 PCT/AU97/00316 6 nucleic acid sequence in the sample. Methods to detect or sense specific NA sequences using the impedance bridge described in International Patent Application Nos PCT/AU88/00273, PCT/AU88/00273, PCT/AU89/00352, PCT/AU90/00025 and PCT/AU92/00132, may, for example, be achieved by manufacturing the membrane with both biotinylated membrane spanning lipids (MSL) and biotinylated gramicidin. Streptavidin, which binds strongly to biotin is immobilized by reaction with these biotinylated compounds.
A mixture of two biotinylated oligonucleotide sequences (first and second ligands) are then attached to the streptavidin. These sequences are preferably non complementary. One sequence would be a suitable probe for a sequence of interest an infectious organism probe sequence, and the other sequence would be the complement to another part of the target DNA.
Upon addition of the DNA to be detected, cross linking of the two biotinylated sequences occurs if the DNA contains the sequences which hybridise to the two attached sequences but not otherwise.
In a fourth aspect the present invention consists in a method of detecting the presence of a selected nucleic acid within a sample, the method comprising the following steps: providing a biosensor comprising an electrode and a bilayer membrane having a top and a bottom layer, the bottom layer being proximal to and connected to the electrode in a manner such that a space exists between the membrane and the electrode, the conductance or impedance of the membrane being dependent on the presence or absence or the selected nucleic acid sequence, the membrane comprising a closely packed array of amphiphilic molecules and a plurality of ion channels comprising first half membrane spanning monomers dispersed in the top layer and second half membrane spanning monomers dispersed in the bottom layer, the first half membrane spanning monomers being capable of lateral diffusion within the upper layer and the second half membranespanning monomers being prevented from lateral diffusion within the bottom layer, a first ligand specifically reactive with a first nucleic acid sequence attached to an end of a proportion of the first half membrane-spanning monomers proximal the surface of the membrane and a second ligand specifically reactive with a second nucleic acid sequence attached to an end of the remainder of the first WO 97/44651 PCT/AU97/00316 7 half membrane-spanning monomers proximal the surface of the membrane, either the first or second nucleic acid sequence being within the sequence of a selected nucleic acid; (ii) adding a sample suspected to contain the selected nucleic acid to the biosensor and measuring the conductance and/or impedance of the membrane; and (iii-) adding a challenge nucleic acid to the biosensor from (ii) and measuring the conductance and/or impedance of the membrane, the challenge sequence being selected such that it includes both the first and second nucleic acid sequences.
If the target nucleic acid is present in the sample, the challenge sequence is unable to bind to both the first and second ligands and thus, crosslink. An absence of a difference in the conductance/impedance measurements in (ii) and (iii) being indicative of the presence of the selected nucleic acid sequence in the sample.
In a fifth aspect the present invention consists in a method of detecting the presence of a selected nucleic acid sequence within a sample, the method comprising the following steps: providing a biosensor comprising an electrode and a bilayer membrane having a top and a bottom layer, the bottom layer being proximal to and connected to the electrode in a manner such that a space exists between the membrane and the electrode, the conductance or impedance of the membrane being dependent on the presence or absence or the selected nucleic acid sequence, the membrane comprising a closely packed array of amphiphilic molecules, membrane spanning amphiphiles which are prevented from lateral diffusion within the membrane and a plurality of ion channels comprising first half membrane spanning monomers dispersed in the top layer and second half membrane spanning monomers dispersed in the bottom layer, the first half membrane spanning monomers being capable of lateral diffusion within the upper layer and the second half membrane-spanning monomers being prevented from lateral diffusion within the bottom layer, a first ligand specifically reactive with the selected nucleic acid sequence attached to either an end of at least a proportion of the first half membrane-spanning monomers proximal the surface of the WO 97/44651 PCT/AU97/00316 8 membrane or to an end of the membrane-spanning amphiphiles proximal the surface of the membrane and a second ligand specifically reactive with a second nucleic acid sequence attached to the other of the at least a proportion of the first half membranespanning monomers proximal the surface of the membrane or an end of the membrane-spanning amphiphiles proximal the surface of the membrane; (ii) adding a sample suspected to contain the selected nucleic acid to the biosensor and measuring the conductance and/or impedance of the membrane; and (iii-) adding a challenge nucleic acid to the biosensor from (ii) and measuring the conductance and/or impedance of the membrane, the challenge sequence being selected such that it includes both the first and second nucleic acid sequences.
If the target nucleic acid is present in the sample, the challenge sequence is unable to bind to both the first and second ligands and thus, crosslink. An absence of a difference in the conductance/impedance measurements in (ii) and (iii) being indicative of the presence of the selected -nucleic acid sequence in the sample.
In a sixth aspect the present invention consists in a biosensor for use in detecting the presence of a selected nucleic acid sequence in a sample, the biosensor comprising an electrode and a bilayer membrane having a top and a bottom layer, the bottom layer being proximal to and connected to the electrode in a manner such that a space exists between the membrane and the electrode, the conductance or impedance of the membrane being dependent on the presence or absence of the selected nucleic acid sequence, the membrane comprising a closely packed array of amphiphilic molecules and a plurality of ion channels comprising first half membrane spanning monomers dispersed in the top layer and second half membrane spanning monomers dispersed in the bottom layer, the first half membrane spanning monomers being capable of lateral diffusion within the upper layer and the second half membrane-spanning monomers being prevented from lateral diffusion within the bottom layer, a first ligand specifically reactive with the selected nucleic acid sequence attached to an end of a proportion of the first half membrane-spanning monomers proximal the surface of the membrane and a second ligand attached to an end of the remainder of the first half WO 97/44651 PCT/AU97/00316 9 membrane-spanning monomers proximal the surface of the membrane, the second ligand having bound thereto or included therein the selected nucleic acid sequence such that the first ligands binds to the second ligand.
In a seventh aspect the present invention consists in a biosensor for use in detecting the presence of a selected nucleic acid sequence in a sample, the biosensor comprising an electrode and a bilayer membrane having a top and a bottom layer, the bottom layer being proximal to and connected to the electrode in a manner such that a space exists between the membrane and the electrode, the conductance or impedance of the membrane being dependent on the presence or absence of the selected nucleic acid sequence, the membrane comprising a closely packed array of amphiphilic molecules, membrane spanning amphiphiles which are prevented from lateral diffusion within the membrane and a plurality of ion channels comprising first half membrane spanning monomers dispersed in the top layer and second half membrane spanning monomers dispersed in the bottom layer, the first half membrane spanning monomers being capable of lateral diffusion within the upper layer and the second half membrane-spanning monomers being prevented from lateral diffusion within the bottom layer, a first ligand specifically reactive with the selected nucleic acid sequence attached to either an end of at least a proportion of the first half membrane-spanning monomers proximal the surface of the membrane or to an end of the membrane-spanning amphiphiles proximal the surface of the membrane and a second ligand attached to the other of the at least a proportion of the first half membrane-spanning monomers proximal the surface of the membrane or the end of the membrane-spanning amphiphiles proximal the surface of the membrane, the second ligand having bound thereto or included therein the selected nucleic acid sequence such that the first ligands binds to the second ligand.
In the arrangements specified in the sixth and seventh aspects the biosensor detects the presence of the selected nucleic acid sequence by competition. The selected nucleic acid sequence, if present in sample to be tested, competes with the second ligand for binding to the first ligand.
In an eighth aspect the present invention consists in a biosensor for use in detecting the presence of a selected nucleic acid sequence in a sample, the biosensor comprising an electrode and a bilayer membrane having a top and a bottom layer, the bottom layer being proximal to and connected to the WO 97/44651 PCT/AU97/00316 electrode in a manner such that a space exists between the membrane and the electrode, the conductance or impedance of the membrane being dependent on the presence or absence of the selected nucleic acid sequence, the membrane comprising a closely packed array of amphiphilic molecules and a plurality of ion channels comprising first half membrane spanning monomers dispersed in the top layer and second half membrane spanning monomers dispersed in the bottom layer, the first half membrane spanning monomers being capable of lateral diffusion within the upper layer and the second half membrane-spanning monomers being prevented from lateral diffusion within the bottom layer and a first ligand specifically reactive with the selected nucleic acid sequence attached to an end of a proportion of the first half membrane-spanning monomers proximal the surface of the membrane.
In this arrangement upon addition of sample containing the selected nucleic acid sequence the first ligand will bind to the selected DNA sequence and change the mobility of the first half membrane monomers or restrict ion flow through the channel. This will result in a membrane with a different conductance.
In another preferred embodiment of the invention, the first and second half membrane spanning monomers are gramicidin or one of its derivatives.
In a further preferred embodiment of the present invention, the bilayer membrane is attached to the electrode via linking molecules such that a space exists between the membrane and the electrode. Preferred linking molecules are those disclosed in application PCT/AU92/00132. In yet a further preferred embodiment of the present invention, the second half membrane spanning monomers are attached to the electrode via linker groups.
In yet another preferred embodiment of the present invention, the bilayer membrane includes membrane spanning lipids, similar to those found in archaebacteria.
"Cross linking" is characterized by the cross linking sequence bringing the first half membrane spanning monomers out of alignment with the second half membrane spanning monomers. This results in a membrane having a second conductance which is different from the first conductance.
WO 97/44651 PCT/AU97/00316 11 This difference in conductance can be measured and will be indicative of the presence of the selected DNA sequence in the sample.
The first and second ligands may be the same or different.
Oligonucleotide ligands which bind to specific NA sequences are well known in the art.
In an alterative embodiment of the present invention the ligands are peptide nucleic acids (PNA). Information regarding PNA may be found in Science, 1991, 254, 1497 1500; J. Am. Chem. Soc., 1992, 114, 1895 1897, J.
Am. Chem. Soc., 1992, 114, 9677 78; J. Chem. Soc. Chem. Commun., 1993, 800 801. Nature, 1993, 365, 566, 68; US Patent No. 4,795,700 and International Patent Application No. WO 93/18187 and the disclosure of these documents is included herein by reference. There are a number of advantages gained by the use of PNA as the ligands. These include the ability of PNA to recognize specific sequences in double stranded DNA and to bind thereto either by strand displacement or triple helix formation. This is in contrast-to oligonucleotides which can bind single strand or double strand but not by strand displacement. It should be noted that PNA can also bind to single stranded DNA. The target probe sequence could be a PNA molecule for tighter binding.
In an alterative embodiment, a mixture of two biotinylated oligonucleotide sequences are immobilized onto streptavidin. One is the target probe sequence and the second is any appropriate cross linking sequence of choice. On addition of sample, the target analyte is captured.
The target analyte need only have the sequence of interest. No cross linking occurs, therefore gating is not observed and a first measurement of conductance is taken. A second oligonucleotide sequence is then used to challenge the system. This challenge sequence contains both the target oligonucleotide sequence and the complementary sequence to the cross linking sequence. If binding of both epitopes occurs, cross linking will take place, thus gating off the ion channels and changing the conductance of the membrane. If binding of only epitope two occurs (owing to the binding of the target DNA to its probe sequence) no cross linking takes place, thus no gating is seen. An absence of a change in conductance following addition of the challenge sequence is therefor an indication of the presence of the target analyte.
WO 97/44651 PCT/AU97/00316 12 The NA probe sequence, in principle, can be of any length and will contain the base sequence complementary to the target analyte for detection.
The target analyte can be a DNA or an RNA sequence. A PNA probe sequence may also be of any length and will contain the base sequence complementary to the target analyte for detection. The target analyte can be a DNA or an RNA sequence as PNA binds to both. PNA binds DNA and RNA more tightly than does DNA, therefore the challenge sequence should not be able to displace the already bound target sequence.
Some examples of target sequences are listed below.
Listeria monocytogenes; a 19 base pair oligonucleotide that is specific for a 36S rRNA sequence; detection in food industry (milks, cheeses etc.) See: R. F. Wang W. W. Cao and M.G. Johnson, App. Environ.
Microbiol., 1991, 3666 All cholera toxin producing Strains of Vibrio cholerea c hybridise specifically with a 23 base pair oligonucleotide sequence (water detection, health). See: A C. Wright et al., J. Clin. Microbiol., 1992, 2302.
Specific oligonucleotide probes for detection of various strains of Chiamydia and Gonorrhoea (health industry). See: R. Rossau et al, u. Clin. Microbiol., 1990, 944 8; J. Gen. Microbiol, 1989, 135, 1735 Loeffelholz, M. J, J. Clin. Microbiol., 1992,2847 51.
An excellent summary of current technologies that use DNA probes is found In "DNA Probes", 2nd Ed.: G. H Keller M. M. Manak, Stockton Press, 1993.
In order that the nature of the present invention may be more clearly understood preferred forms thereof will now be described with reference to the following non-limiting examples.
Example 1: Lateral Segregation of DNA Detection of target polynucleotide sequences can be illustrated by the following example in which specific DNA gating is observed for a target DNA concentration of 5 nM (1 pmole). The total lack of any response in the presence of a non-complementary probe sequence can be seen in the controls.
WO 97/44651 PCT/AU97/00316 13 1st layer: 9.3nM Linker Gramicidin B 1.1pM Membrane spanner Lipid D 27.5nM Membrane spanner Lipid C 37pM dithio diglyconic acid Linker Lipid A 2nd layer: 14mM (DPE-PC:GDPE= 7:3) Biotinylated Gramicidin E 50,000:1 in ethanol Electrodes with freshly evaporated gold (1000A) on chrome adhesion layer (200A) on glass microscope slides) were dipped into an ethanolic solution of the first layer components for 1 hr at room temperature, rinsed with ethanol, then stored at 4 0 C under ethanol until used for impedance measurements. The slide was clamped into a block containing teflon coated wells which defined the area of the working electrode as approximately 16mm 2 All steps were carried out at room temperature. 5p.L of the 2nd layer was added to the working electrode before addition of a 180PL volume of phosphate buffered saline (lOmM Na 2
HPO
4 1mM KH 2
PO
4 137mM NaC1, 2.7mM KC1, PBS). The electrode was washed 4 times using PBS.
Streptavidin was added to all wells (5gL 0.0lmg/mL in PBS) and allowed to react with biotinylated Gramicidin E for 10-15 minutes before washing out excess unbound streptavidin with PBS. 5iL of a 1:1 mixture of DNA probe F (200nM):DNA probe G (200nM in PBS) was added to sensor wells. A DNA non-specific binding probe H (5gL 400 nM in PBS) was added to control wells. The probes were allowed to react with streptavidin for 10-15 minutes then excess unbound probes were washed out with PBS. 100L of DNA target I (lOnM) in PBS was added to each well. The binding of DNA target I to the sensor wells gave a decrease in the admittance at minimum phase, but no significant change in membrane admittance in control wells (Figure 1).
The amount and rate of decrease of admittance at minimum phase is related to the amount of DNA present in the test solution and therefore can be used to determine concentration in test solutions.
WO 97/44651 PCT/AU97/00316 14 DNA probe F: listeria probe DNA with a 31-atom phosphoramidite linker group between the biotin and DNA.
5'-bio-L-M-ATAGTTTTATGGGATTAGC-3' DNA probe G: cholera toxin probe DNA with a 31-atom phosphoramidite linker group between the biotin and DNA.
5'-bio-L-M-CTCCGGAGCATAGAGCTTGGAGG-3' DNA non-specific binding probe H: 15-mer oligonucleotide with a 31-atom phosphoramidite linker group between the biotin and DNA, which is non-complementary to all parts of the target DNA sequence.
5'-bio-L-M-ATTGCTACGTATACG-3' DNA target I: 67 base DNA sequence containing the 19-base listeria sequence, a base 'spacer' and the 23 base cholera toxin sequence.
CCCTCCAAGCTCTATGCTCCGGAG-3' where: bio biotin
L=
0 NH
O-DMT
O
0-P-O
II
0 WO 97/44651 PCT/AU97/00316
M=
0O 0 0- 0 0 Q 0 0- I
I
Example 2: Lateral Segregation of DNA followed by enzymatic cleavage This example illustrates the ability to perform a second, confirmatory step whereby an enzyme can be used to cleave the bound polynucleotide, thus confirming that a specific region of interest is present.
1st layer: 9.3nM Linker Gramicidin B l.luM Membrane spanner Lipid D 27.5nM Membrane spanner Lipid C 37uM dithio diglyconic acid 75uM Linker Lipid A 2nd layer: 14mM (DPE-PC:GDPE= 7:3) Biotinylated Gramicidin E 50,000:1 in ethanol Electrodes were prepared and 2 nd layer added as described in Example 1.
Subsequent streptavidin, DNA probe and DNA target sequences were added as described in Example 1, except that they were carried out at 30 0
C.
After 15 minutesof DNA gating, unbound DNA target I was washed out with DNase I activation buffer. DNase I activation buffer consists of 50 nM Tris.HC1, pH 7.6, 50 nM NaCl, 10 nM MgCl 2 10 nM MnClI, 0.2 mg/mL BSA.
DNase I was added (2 iL Img/mL in a 50%w/v glycerol solution of 20 mM Tris.HCl, pH 7.6, 1 mM MgCl 2 to sensor and control wells. Addition of DNase I gave an increase in admittance at minimum phase for sensor wells, but no significant increase for control wells (figure showing that cleavage of DNA was being detected.
WO 97/44651 PCT/AU97/00316 16 Example 3: DNA gating by addition of 2 biotinylated complementary polynucleotide sequences This example illustrates the possibility of detecting only one specific sequence on the polynucleotide of interest by having a capture molecule incorporated in the target. It also illustrates the use of peptide nucleic acid (PNA) sequences.
1st layer: 9.3nM Linker Gramicidin B 1.1uM Membrane spanner Lipid D Membrane spanner Lipid C 37uM dithio diglyconic acid Linker Lipid A 2nd layer: 14mM (DPE-PC:GDPE=7:3) Biotinylated Gramicidin E 50,000:1 in ethanol Electrodes were prepared and 2 nd layer added as described in Example 1. All steps were carried out at room temperature. 54L of the 2nd layer was added to the working electrode before addition of a 180atL volume of phosphate buffered saline (10mM Na2HPO 4 ImM KH 2
PO
4 137mM NaC1, 2.7mM KC1, PBS). The electrode was washed 4 times using PBS.
Streptavidin was added to all wells (5p.L 0.0lmg/mL in PBS) and allowed to react with biotinylated Gramicidin E for 10-15 minutes before washing out excess unbound streptavidin with PBS.
5 nM of each of PNA sequence J and the complementary DNA sequence K (1.14gL 1.75 apM) were added quickly in succession to the sensor wells. No PNA or DNA sequence was added to the control wells. The binding of the PNA and DNA sequences to the streptavidin and each other gave a decrease in the admittance at minimum phase, but no significant change in membrane admittance in control wells (Figure The amount and rate of decrease of admittance at minimum phase is related to the amount of DNA or PNA present in the test solution and therefore can be used to determine concentration in test solutions.
WO 97/44651 PCT/AU97/00316 17 PNA sequence J: N-biotinylated listeria probe sequence PNA containing glycine and lysine for solubility plus an 8-amino-3,6-dioxaoctanoic acid linker.
N-bio-gly-lys-O-ATAGTTTTATGGGATTAGC-CONH 2 DNA sequence K: listeria DNA with no linker group between the biotin and DNA.
5'-bio-GCTAATCCCATAAAACTAT-3' where 0= 0
N.
"o N It will be appreciated by persons skilled in the art that numerous variations and/or modifications may be made to the invention as shown in the specific embodiments without departing from the spirit or scope of the invention as broadly described. These include the detection of PCR (or other amplification method) products and the detection of genetic mutations in genetically derived diseases, where the mutation is known. A list of some 3500 conditions due to defective genes can be found in McKusick's "Mendelian Inheritance in Man" and further examples are regularly reported in the scientific press. The present embodiments are, therefore, to be considered in all respects as illustrative and not restrictive.
Claims (18)
1. A biosensor for use in detecting the presence of a selected nucleic sequence in a sample, the biosensor comprising an electrode and a bilayer membrane having a top and a bottom layer, the bottom layer being proximal to and connected to the electrode in a manner such that a space exists between the membrane and the electrode, the conductance or impedance of the membrane being dependent on the presence or absence of the selected nucleic acid sequence, the membrane comprising a closely packed array of amphiphilic molecules and a plurality of ion channels comprising first half membrane spanning monomers dispersed in the top S: layer and second half membrane spanning monomers dispersed in the S. bottom layer, the first half membrane spanning monomers being capable of lateral diffusion within the upper layer and the second half membrane- spanning monomers being prevented from lateral diffusion within the bottom layer, a first ligand specifically reactive with the selected nucleic acid sequence attached to an end of a proportion of the first half membrane- spanning monomers proximal the surface of the membrane and a second ligand reactive with the selected nucleic acid or marker attached thereto is 20 attached to an end of the remainder of the first half membrane-spanning monomers proximal the surface of the membrane.
2. A biosensor for use in detecting the presence of a selected nucleic acid sequence in a sample, the biosensor comprising an electrode and a bilayer membrane having a top and a bottom layer, the bottom layer being 25 proximal to and connected to the electrode in a manner such that a space exists between the membrane and the electrode, the conductance or impedance of the membrane being dependent on the presence or absence of the selected nucleic acid sequence, the membrane comprising a closely packed array of amphiphilic molecules and a plurality of ion channels comprising first half membrane spanning monomers dispersed in the top layer and second half membrane spanning monomers dispersed in the bottom layer, the first half membrane spanning monomers being capable of lateral diffusion within the upper layer and the second half membrane- spanning monomers being prevented from lateral diffusion within the bottom layer, a first ligand specifically reactive with the selected nucleic acid sequence attached to an end of a proportion of the first half membrane- 06/04 '01 FRI 09:41 [TX/RX NO 8998] 06/04 '01 10:46 FAX 61 3 9663 3099 FB RICE CO. oo005 WO 97/44651 PCT/AU97/00316 19 spanning monomers proximal the surface of the membrane-and a second ligand attached to an end of the remainder of the first half membrane- spanning monomers proximal the surface of the membrane, the second ligand having bound thereto or included therein the selected nucleic acid sequence such that the first ligands binds to the second ligand.
3. A biosensor as claimed in claim 1 or claim 2 in which the membrane includes membrane spanning amphiphiles which are prevented from lateral diffusion within the membrane.
4. A biosensor for use in detecting the presence of a selected nucleic sequence in a sample, the biosensor comprising an electrode and a bilayer membrane having a top and a bottom layer, the bottom layer being proximal to and connected to the electrode in a manner such that a space exists between the membrane and the electrode, the conductance or impedance of the membrane being dependent on the presence or absence or 15 the selected nucleic acid sequence, the membrane comprising a closely packed array of amphiphilic molecules, membrane spanning amphiphiles which are prevented from lateral diffusion within the membrane and a plurality of ion channels comprising first half membrane spanning monomers dispersed in the top layer and second half membrane spanning monomers 20 dispersed in the bottom layer, the first half membrane spanning monomers being capable of lateral diffusion within the upper layer and the second half membrane-spanning monomers being prevented from lateral diffusion within Sthe bottom layer, a first ligand specifically reactive with the selected nucleic acid sequence attached to either an end of at least a proportion of the first 25 half membrane-spanning monomers proximal the surface of the membrane or to an end of the membrane-spanning amphiphiles proximal the surface of the membrane and a second ligand reactive with the selected nucleic acid or marker attached thereto attached to the other of the at least a proportion of the first half membrane-spanning monomers proximal the surface of the membrane or the end of the membrane-spanning amphiphiles proximal the surface of the membrane. A biosensor for use in detecting the presence of a selected nucleic acid sequence in a sample, the biosensor comprising an electrode and a bilayer membrane having a top and a bottom layer, the bottom layer being proximal to and connected to the electrode in a manner such that a space exists between the membrane and the electrode, the conductance or 06/04 '01 FRI 09:41 [TX/RX NO 8998] WO 97/44651 PCT/AU97/00316 impedance of the membrane being dependent on the presence or absence of the selected nucleic acid sequence, the membrane comprising a closely packed array of amphiphilic molecules, membrane spanning amphiphiles which are prevented from lateral diffusion within the membrane and a plurality of ion channels comprising first half membrane spanning monomers dispersed in the top layer and second half membrane spanning monomers dispersed in the bottom layer, the first half membrane spanning monomers being capable of lateral diffusion within the upper layer and the second half membrane-spanning monomers being prevented from lateral diffusion within the bottom layer, a first ligand specifically reactive with the selected nucleic acid sequence attached to either an end of at least a proportion of the first half membrane-spanning monomers proximal the surface of the membrane or to an end of the membrane-spanning amphiphiles proximal the surface of the membrane and a second ligand attached to the other of the at least a proportion of the first half membrane-spanning monomers proximal the surface of the membrane or the end of the membrane-spanning amphiphiles proximal the surface of the membrane, the second ligand having bound thereto or included therein the selected nucleic acid sequence such that the first ligands binds to the second ligand.
6. A biosensor as claimed in any one of claims 1 to 5 in which the first ligand comprises a nucleic acid molecule or PNA which includes a sequence complementary to a first sequence within the selected nucleic acid.
7. A biosensor as claimed in any one of claims 1 to 6 in which the second ligand is an antibody directed against DNA, or binding fragment thereof.
8. A biosensor as claimed in any one of claims 1 to 6 in which the second ligand is an intercalating agent, major or minor groove binder, an agent capable of triple helix formation or an agent which can covalently couple to DNA.
9. A biosensor as claimed in any one of claims 1 to 6 in which the second ligand is specific for the selected nucleic acid sequence A biosensor as claimed in any one of claims 1 to 9 in which the first and second ligands are attached via linkers to the ion channels or membrane spanning lipids.
11. A biosensor as claimed in claim 10 in which the linker is a phosphoramidite linker group. 06/04 '01 10:47 FAX 61 3 9663 3099 FB RICE CO. o006 WO 97/44651 PCT/AU971003 16 21
12. A biosensor for use in detecting the presence of a selected nucleic acid sequence in a sample, the biosensor comprising an electrode and a bilayer membrane having a top and a bottom layer, the bottom layer being proximal to and connected to the electrode in a manner such that a space exists between the membrane and the electrode, the conductance or impedance of the membrane being dependent on the presence or absence of the selected nucleic acid sequence, the membrane comprising a closely packed array of amphiphilic molecules and a plurality of ion channels comprising first half membrane spanning monomers dispersed in the top layer and second half membrane spanning monomers dispersed in the bottom layer, the first half membrane spanning monomers being capable of lateral diffusion within the upper layer and the second half membrane- spanning monomers being prevented from lateral diffusion within the bottom layer and a first ligand specifically reactive with the selected nucleic acid 15 sequence attached to an end of a proportion of the first half membrane- spanning monomers proximal the surface of the membrane. 0 13. A biosensor as claimed in claim 12 in which the first ligand comprises a nucleic acid molecule or PNA which includes a sequence complementary to a first sequence within the selected nucleic acid. 20 14. A biosensor as claimed in claim 12 or 13 in which the first ligand is attached via linkers to the ion channels. 15, A biosensor as claimed in claim 14 in which the linker is a phosphoramidite linker group,
16. A biosensor as claimed in any one of claims 1 to 15 in which the first 25 and second half membrane spanning monomers are gramicidin or one of its derivatives.
17. A method of detecting the presence of a selected nucleic acid within a sample, the method comprising the following steps: providing a biosensor comprising an electrode and a bilayer membrane having a top and a bottom layer, the bottom layer being proximal to and connected to the electrode in a manner such that a space exists between the membrane and the electrode, the conductance or impedance of the membrane being dependent on the presence or absence pfthe selected nucleic acid sequence, the membrane comprising a closely packed array of amphiphilic molecules and a plurality of ion channels comprising first half 06/04 '01 FRI 09:41 [TX/RX NO 8998] WO 97/44651 PCT/AU97/00316 22 membrane spanning monomers dispersed in the top layer and second half membrane spanning monomers dispersed in the bottom layer, the first half membrane spanning monomers being capable of lateral diffusion within the upper layer and the second half membrane- spanning monomers being prevented from lateral diffusion within the bottom layer, a first ligand specifically reactive with a first nucleic acid sequence attached to an end of a proportion of the first half membrane-spanning monomers proximal the surface of the membrane and a second ligand specifically reactive with a second nucleic acid sequence attached to an end of the remainder of the first half membrane-spanning monomers proximal the surface of the membrane, either the first or second nucleic acid sequence being within the sequence of a selected nucleic acid; (ii) adding a sample suspected to contain the selected nucleic acid to the biosensor and measuring the conductance and/or impedance of the membrane; and (iii-) adding a challenge nucleic acid to the biosensor from (ii) and measuring the conductance and/or impedance of the membrane, the challenge sequence being selected such that it includes both the first and second nucleic acid sequences.
18. A method of detecting the presence of a selected nucleic acid sequence within a sample, the method comprising the following steps: providing a biosensor comprising an electrode and a bilayer membrane having a top and a bottom layer, the bottom layer being proximal to and connected to the electrode in a manner such that a space exists between the membrane and the electrode, the conductance or impedance of the membrane being dependent on the presence or absence or the selected nucleic acid sequence, the membrane comprising a closely packed array of amphiphilic molecules, membrane spanning amphiphiles which are prevented from lateral diffusion within the membrane and a plurality of ion channels comprising first half membrane spanning monomers dispersed in the top layer and second half membrane spanning monomers dispersed in the bottom layer, the first half membrane spanning monomers being capable of lateral diffusion within the upper layer and the second half membrane-spanning monomers being WO 97/44651 PCT/AU97/00316 23 prevented from lateral diffusion within the bottom layer, a first ligand specifically reactive with the selected nucleic acid sequence attached to either an end of at least a proportion of the first half membrane-spanning monomers proximal the surface of the membrane or to an end of the membrane-spanning amphiphiles proximal the surface of the membrane and a second ligand specifically reactive with a second nucleic acid sequence attached to the other of the at least a proportion of the first half membrane- spanning monomers proximal the surface of the membrane or an end of the membrane-spanning amphiphiles proximal the surface of the membrane; (ii) adding a sample suspected to contain the selected nucleic acid to the biosensor and measuring the conductance and/or impedance of the membrane; and (iii-) adding a challenge nucleic acid to the biosensor from (ii) and measuring the conductance and/or impedance of the membrane, the challenge sequence being selected such that it includes both the first and second nucleic acid sequences.
19. A method as claimed in claims 17 or 18 in which the first ligand comprises a nucleic acid molecule or PNA which includes a sequence complementary to a first sequence within the selected nucleic acid. A method as claimed in any one of claims 17 to 19 in which the second ligand is an antibody directed against DNA, or binding fragment thereof.
21. A method as claimed in any one of claims 17 to 19 in which the second ligand is an intercalating agent, major or minor groove binder, an agent capable of triple helix formation or an agent which can covalently couple to DNA.
22. A method as claimed in any one of claims 17 to 19 in which the second ligand is specific for the selected nucleic acid sequence
23. A method as claimed in any one of claims 17 to 23 in which the first and second ligands are attached via linkers to the ion channels or membrane spanning lipids.
24. A method as claimed in claim 23 in which the linker is a phosphoramidite linker group. WO 97/44651 PCT/AU97/00316 A method as claimed in any one of claims 17 to 24 in which the first and second half membrane spanning monomers are gramicidin or one of its derivatives.
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AUPN366895A0 (en) * | 1995-06-20 | 1995-07-13 | Australian Membrane And Biotechnology Research Institute | Detection of small analytes |
US6127127A (en) * | 1995-06-27 | 2000-10-03 | The University Of North Carolina At Chapel Hill | Monolayer and electrode for detecting a label-bearing target and method of use thereof |
US6387625B1 (en) | 1995-06-27 | 2002-05-14 | The University Of North Carolina At Chapel Hill | Monolayer and electrode for detecting a label-bearing target and method of use thereof |
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- 1997-05-22 EP EP97921532A patent/EP0990149A4/en not_active Withdrawn
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EP0990149A1 (en) | 2000-04-05 |
JP2000513811A (en) | 2000-10-17 |
WO1997044651A1 (en) | 1997-11-27 |
AU2757897A (en) | 1997-12-09 |
EP0990149A4 (en) | 2003-02-26 |
CA2255952A1 (en) | 1997-11-27 |
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