AU2007214362B2 - KGF polypeptide compositions - Google Patents

Description

AUSTRALIA FB RICE & CO Patent and Trade Mark Attorneys Patents Act 1990 CHIRON CORPORATION COMPLETE SPECIFICATION STANDARD PATENT Invention Title. KGF polypeptide compositions The following statement is a full description of this invention including the best method of performing it known to us:- KGF POLYPEPTIDE COMPOSITIONS This is a divisional of AU 2002326742, the entire contents of which are 5 incorporated herein by reference. Technical Field The present invention relates generally to polypeptide growth factors. Specifically, the invention relates to compositions comprising keratinocyte growth 10 factor polypeptides and methods of using the same. Background of the Invention Keratinocyte growth factor (KGF) belongs to the family of fibroblast growth factors ("FGFs"), the prototypes of which are represented by basic FGF and acidic 15 FGF. KGF is also known as FGF-7. KGF, like FGFs, binds heparin and is generally capable of stimulating the proliferation and differentiation of a variety of cell types derived from the primary or secondary mesoderm as well as from neuroectoderm. For example, KGF, like FGFs, has the ability to induce the differentiation and proliferation of ventral as well as dorsal mesoderm in early blastulae. See, e.g., Gospodarowicz et. 20 al. Cell. Biol. Rev. (1991) 25: 307-314; and Basilico et al. Adv. Cancer Res. (1992) 59: 115-165. Like other FGFs, KGF is a heparin-binding protein, but unlike other FGFs, it has a unique target cell specificity. Particularly, KGF is similar to other FGFs in its ability to stimulate epithelial cell proliferation, but is dissimilar to other FGFs in its 25 inability to stimulate endothelial cells or fibroblast proliferation. See, e. g. , Finch, et. al. Science (1989) 245: 752-755. Mature, full-length KGF, designated herein as KGF 163, is a polypeptide with 163 amino acid residues, and possesses a potential N glycosylation site that extends from amino acid residue 14 to 16 at the N-terminus. Finch, et. al. Science (1989) 245: 752-755. 30 1 A Ron et al. J. Biol. Chem. (1993) 268:2984-2988 found that when KGF 163 was expressed in a prokaryotic expression system, a recombinant KGF ("rKGF") polypeptide could be obtained that possessed mitogenic activity. When the rKGF molecule was truncated by deletion of 3, 8, 27, 38, and 48 amino acid residues from the 5 N-terminus of the mature KGF 1 63 polypeptide, biological activity of the resulting molecules varied. With deletion of 3 and 8 amino acid residues, respectively, the mitogenic activity of the resulting molecules did not appear to be affected as compared to full-length rKGF. Deletion of 27 amino acid residues, however, resulted in molecules that displayed 10-20 fold reduced mitogenic activity. Deletion of 38 and 48 10 amino acid residues, respectively, resulted in complete loss of mitogenic activity and heparin-binding ability. Ron et al., however, failed to produce any truncated rKGF fragments that possessed increased mitogenic activity as compared to the full-length rKGF molecule. U.S. Patent Nos. 5,677,278, 5,773,586, 5,843,883, 5,863,767 and 6,074,848, all 15 to Gospodarowicz et al., describe KGF molecules. One particular molecule, with an N terminal deletion of 23 amino acid residues, termed KGF desl-23, demonstrates enhanced mitogenic activity as compared to mature, full-length recombinant KGF 63 . Osslund et al. Protein Sci. (1998) 7:1681-1690 reports various N-terminal truncated KGF molecules and certain measurements of their mitogenic activity. 20 Similarly, International Publications WO 96/11951 and WO 96/11949 describe KGF molecules with various N-terminal truncations and amino acid substitutions. Any discussion of documents, acts, materials, devices, articles or the like which has been included in the present specification is solely for the purpose of providing a context for the present invention. It is not to be taken as an admission that any or all of 25 these matters form part of the prior art base or were common general knowledge in the field relevant to the present invention as it existed before the priority date of each claim of this application. Disclosure of the Invention 30 The present invention is based on the discovery that various N-terminally truncated KGF polypeptides, and analogs thereof, display enhanced biological activity on a per molecule basis relative to native, full-length KGF 1 63 . Thus, compositions containing these molecules have increased potency for the treatment of conditions where epithelialization is required, such as for the treatment of wounds, burns, 35 ophthalmic disorders, gastrointestinal diseases and any disorder where stimulation of epithelial cell proliferation or regeneration is desired. These molecules can be delivered alone or in combination with other mitogenic agents, such as other growth factors, including for example, any of the other FGFs, as well as platelet derived growth factor (PDGF), epidermal growth factor (EGF), insulin-like growth factor (IGF), insulin-like growth factor binding proteins (IGFBPs), and the like. Moreover, these KGF molecules can be conjugated to toxin molecules in order to 5 target these toxins to epithelial cells in order to treat hyperproliferative diseases. Accordingly, in one embodiment, the subject invention is directed to a method of stimulating epithelial cell proliferation. The method comprises contacting epithelial cells with a composition comprising: (a) a therapeutically effective amount of a KGF polypeptide, wherein the KGF 10 polypeptide is selected from the group consisting of: (i) KGFd,,I-IS consisting of the contiguous amino acid sequence depicted at amino acid residues 16-163, inclusive, of Figure 1; (ii) KGFdC,,.IS consisting of the contiguous amino acid sequence depicted at amino acid residues 19-163, inclusive, of Figure 1; 15 (iii) KGFde,., consisting of the contiguous amino acid sequence depicted at amino acid residues 20-163, inclusive, of Figure 1; (iv) KGFdei 2 0 consisting of the contiguous amino acid sequence depicted at amino acid residues 21-163, inclusive, of Figure 1; (v) KGFeSt- 2 I consisting of the contiguous amino acid sequence depicted at 20 amino acid residues 22-163, inclusive, of Figure 1; (vi) KGFacs- 22 consisting of the contiguous amino acid sequence depicted at amino acid residues 23-163, inclusive, of Figure 1; (vii) KGFes 2 4 consisting of the contiguous amino acid sequence depicted at amino acid residues 25-163, inclusive, of Figure 1; 25 (viii) KGFes- 2 s consisting of the contiguous amino acid sequence depicted at amino acid residues 26-163, inclusive, of Figure 1; (ix) a biologically active analog of (i), (ii), (iii), (iv), (v), (vi), (vii) or (viii), wherein the biologically active analog consists of the same number of amino acids as (i), (ii), (iii), (iv), (v), (vi), (vii) or (viii), respectively, and has at least 70% sequence 30 homology thereto, 3 wherein the KGF polypeptide exhibits an increase in bioactivity relative to mature, full-length, KGF (KGF, 6 3 ) as determined by the Balb/MK bioactivity assay and specifically stimulates epithelial cell proliferation, and further wherein the therapeutically effective amount is 75% or less of the amount on a per molecule basis of KGF, 63 needed 5 to elicit an equivalent therapeutic response; and (b) a pharmaceutically acceptable excipient. In certain embodiments, the biologically active analog has at least 80% or 90% sequence homology to (i), (ii), (iii), (iv), (v), (vi), (vii), (viii) or (ix). In another embodiment, the invention is directed to a method as described above 10 wherein the KGF polypeptide is KGFesi- 22 consisting of the contiguous amino acid sequence depicted at amino acid residues 23-163, inclusive, of Figure 1, or a biologically active analog thereof wherein the biologically active analog consists of 141 amino acids and has at least 70% sequence homology thereto, and wherein the therapeutically effective amount is 50% or less of the amount on a per molecule basis of KGF 6 ,6 needed 15 to elicit an equivalent therapeutic response. In yet further embodiments, the invention is directed to a method as described above wherein the KGF polypeptide is KGFdeI- 2 4 consisting of the contiguous amino acid sequence depicted at amino acid residues 25-163, inclusive, of Figure 1, or a biologically active analog thereof wherein the biologically active analog consists of 139 amino acids 20 and has at least 70% sequence homology thereto, and wherein the therapeutically effective amount is 50% or less of the amount on a per molecule basis of KGF,.

3 needed to elicit an equivalent therapeutic response. In a further embodiment, the invention is directed to a method of stimulating epithelial cell proliferation which comprises contacting epithelial cells with a 25 composition comprising: a) a therapeutically effective amount of a KGF polypeptide, wherein the KGF polypeptide is (i) KGFdeS- 22 consisting of the contiguous amino acid sequence depicted at amino acid residues 23-163, inclusive, of Figure 1, or (ii) a biologically active analog of (i) which consists of the same number of amino acids as (i) and has at least 70% sequence 30 homology thereto, 4 wherein the KGF polypeptide exhibits an increase in bioactivity relative to mature, full-length, KGF (KGF 16 3 ) as determined by the Balb/MK bioactivity assay and specifically stimulates epithelial cell proliferation, and further wherein the therapeutically effective amount is 10% to 75% of the amount on a per molecule basis of KGF 163 needed 5 to elicit an equivalent therapeutic response; and (b) a pharmaceutically acceptable excipient. In certain embodiments, the biologically active analog has at least 80% or 90% sequence homology to (i) or (ii). In other embodiments, the biologically active analog consists of the contiguous 10 amino acid sequence depicted at amino acid residues 23-163, inclusive, of Figure 1 with the N-terminal arginine residue substituted with an alanine residue. In still further embodiments, the therapeutically effective amount is 10% to 20%, or 10% to 25%, or 10% to 50% of the amount on a per molecule basis, or any percentage within these ranges, of the amount of full-length KGF needed to elicit an equivalent 15 therapeutic response. In another embodiment, the invention is directed to a method of stimulating epithelial cell proliferation comprising contacting epithelial cells with a composition comprising: a) a therapeutically effective amount of a KGF polypeptide, wherein the KGF 20 polypeptide is (i) KGFeI- 24 consisting of the contiguous amino acid sequence depicted at amino acid residues 25-163, inclusive, of Figure 1, or (ii) a biologically active analog of (i) which consists of the same number of amino acids as (i) and has at least 70% sequence homology thereto, wherein the KGF polypeptide exhibits an increase in bioactivity relative to 25 mature, full-length, KGF (KGF 163 ) as determined by the Balb/MK bioactivity assay and specifically stimulates epithelial cell proliferation, and further wherein the therapeutically effective amount is 5% to 75% of the amount on a per molecule basis of KGF 1 63 needed to elicit an equivalent therapeutic response; and (b) a pharmaceutically acceptable excipient. 5 In certain embodiments of the method described above, the biologically active analog has at least 80% or at least 90% sequence homology to (i) or (ii). In additional embodiments, the therapeutically effective amount is 5% to 10%, 10% to 20%, 10% to 25%, or 10% to 50%, or any percentage within these ranges, of the 5 amount on a per molecule basis of the amount of full-length KGF needed to elicit an equivalent therapeutic response. In all of the methods described above, epithelial cells may be contacted with the KGF polypeptides in vitro or in vivo. In another embodiment, the invention is directed to a method of treating wounds 10 comprising applying a KGF polypeptide composition to an area of a wound to be treated and allowing the wound to heal. The composition comprises: (a) a therapeutically effective amount of a KGF polypeptide, wherein the KGF polypeptide is selected from the group consisting of: (i) KGFde,-15 consisting of the contiguous amino acid sequence depicted at 15 amino acid residues 16-163, inclusive, of Figure 1; (ii) KGFde.,1-, consisting of the contiguous amino acid sequence depicted at amino acid residues 19-163, inclusive, of Figure 1; (iii) KGFcsl.1 9 consisting of the contiguous amino acid sequence depicted at amino acid residues 20-163, inclusive, of Figure 1; 20 (iv) KGFCSI-20 consisting of the contiguous amino acid sequence depicted at amino acid residues 21-163, inclusive, of Figure 1; (v) KGFdaSI-21 consisting of the contiguous amino acid sequence depicted at amino acid residues 22-163, inclusive, of Figure 1; (vi) KGFdeeS-22 consisting of the contiguous amino acid sequence depicted 25 at amino acid residues 23-163, inclusive, of Figure 1; (vii) KGFCSI 2 4 consisting of the contiguous amino acid sequence depicted at amino acid residues 25-163, inclusive, of Figure 1; (viii) KGFcsI-25 consisting of the contiguous amino acid sequence depicted at amino acid residues 26-163, inclusive, of Figure 1; 6 (ix) a biologically active analog of (i), (ii), (iii), (iv), (v), (vi), (vii) or (viii), wherein the biologically active analog consists of the same number of amino acids as (i), (ii), (iii), (iv), (v), (vi), (vii) or (viii), respectively, and has at least 70% sequence homology thereto, 5 wherein the KGF polypeptide exhibits an increase in bioactivity relative to mature, full-length, KGF (KGF 63 ) as determined by the Balb/MK bioactivity assay and specifically stimulates epithelial cell proliferation, and further wherein the therapeutically effective amount is 75% or less of the amount on a per molecule basis of KGF, 63 needed to elicit an equivalent therapeutic response; and 10 (b) a pharmaceutically acceptable excipient. In certain embodiments of the above method, the biologically active analog has at least 80% or 90% sequence homology to (i), (ii), (iii), (iv), (v), (vi), (vii), (viii) or (ix). In additional embodiments of the above method, the KGF polypeptide is KGFesi.

22 consisting of the contiguous amino acid sequence depicted at amino acid 15 residues 23-163, inclusive, of Figure 1, or a biologically active analog thereof wherein the biologically active analog consists of 141 anino acids and has at least 70% sequence homology thereto, and wherein the therapeutically effective amount is 50% or less of the amount on a per molecule basis of KGF, 63 needed to elicit an equivalent therapeutic response. 20 In other embodiments of the above method, the KGF polypeptide is KGFcSI- 2 4 consisting of the contiguous amino acid sequence depicted at amino acid residues 25-163, inclusive, of Figure 1, or a biologically active analog thereof wherein the biologically active analog consists of 139 amino acids and has at least 70% sequence homology thereto, and wherein the therapeutically effective amount is 50% or less of the amount on 25 a per molecule basis of KGF, needed to elicit an equivalent therapeutic response. In still further embodiments, the invention is directed to a method of treating wounds comprising applying a KGF polypeptide composition to an area of a wound to be treated and allowing the wound to heal, said composition comprising: a) a therapeutically effective amount of a KGF polypeptide, wherein the KGF 30 polypeptide is (i) KGFes.

2 2 consisting of the contiguous amino acid sequence depicted at 7 amino acid residues 23-163, inclusive, of Figure 1, or (ii) a biologically active analog of (i) which consists of the same number of amino acids as (i) and has at least 70% sequence homology thereto, wherein the KGF polypeptide exhibits an increase in bioactivity relative to 5 mature, full-length, KGF (KGF 63 ) as determined by the Balb/MK bioactivity assay and specifically stimulates epithelial cell proliferation, and further wherein the therapeutically effective amount is 10% to 75% of the amount on a per molecule basis of KGF, 63 needed to elicit an equivalent therapeutic response; and (b) a pharmaceutically acceptable excipient. 10 In certain embodiments, the biologically active analog has at least 80% sequence homology or at least 90% sequence homology to (i) or (ii). In additional embodiments of the method above, the biologically active analog consists of the contiguous amino acid sequence depicted at amino acid residues 23-163, inclusive, of Figure 1 with the N-terminal arginine residue substituted with an alanine 15 residue. In still further embodiments, the therapeutically effective amount for use in the methods above is 10% to 20%, or 10% to 25%, or 10% to 50% of the amount on a per molecule basis, or any percentage within these ranges, of the amount of full-length KGF needed to elicit an equivalent therapeutic response. 20 In additional embodiments, the invention is directed to a method of treating wounds comprising applying a KGF polypeptide composition to an area of a wound to be treated and allowing the wound to heal, said composition comprising: a) a therapeutically effective amount of a KGF polypeptide, wherein the KGF polypeptide is (i) KGFe,1.

24 consisting of the contiguous amino acid sequence depicted at 25 amino acid residues 25-163, inclusive, of Figure 1, or a biologically active analog of (i) which consists of the same number of amino acids as (i) and has at least 70% sequence homology thereto, wherein the KGF polypeptide exhibits an increase in bioactivity relative to mature, full-length, KGF (KGF, 63 ) as determined by the Balb/MK bioactivity assay and 30 specifically stimulates epithelial cell proliferation, and further wherein the therapeutically 8 effective amount is 5% to 75% of the amount on a per molecule basis of KGF 16 3 needed to elicit an equivalent therapeutic response; and (b) a pharmaceutically acceptable excipient. In certain embodiments of the above method, the biologically active analog has 5 at least 80% sequence homology or at least 90% sequence homology to (i) or (ii). In still further embodiments, the therapeutically effective amount for use in the method above is 5% to 10%, or 10% to 20%, or 10% to 25%, or 10% to 50% of the amount on a per molecule basis, or any percentage within these ranges, of the amount of full-length KGF needed to elicit an equivalent therapeutic response. 10 In the above methods, the composition may be contacted with the wound in vitro or in vivo. The present invention further provides a method of treating a gastrointestinal disorder in a subject comprising administering to the subject a composition comprising a KGF polypeptide, wherein said KGF polypeptide is selected from the group 15 consisting of: (i) KGFdesi.18 consisting of the contiguous amino acid sequence depicted at amino acid residues 19-163, inclusive, of Figure 1; (ii) KGFdesl.19 consisting of the contiguous amino acid sequence depicted at amino acid residues 20-163, inclusive, of Figure 1; 20 (iii) KGFdesi-20 consisting of the contiguous amino acid sequence depicted at amino acid residues 21-163, inclusive, of Figure 1; (iv) KGFdsI- 2 1 consisting of the contiguous amino acid sequence depicted at amino acid residues 22-163, inclusive, of Figure 1; (v) KGFdes-22 consisting of the contiguous amino acid sequence depicted at 25 amino acid residues 23-163, inclusive, of Figure 1; (vi) KGFdesi-2 4 consisting of the contiguous amino acid sequence depicted at amino acid residues 25-163, inclusive, of Figure 1; (vii) KGFdesi-25 consisting of the contiguous amino acid sequence depicted at amino acid residues 26-163, inclusive, of Figure 1; 30 (viii) a biologically active analog of (i), (ii), (iii), (iv), (v), (vi), or (vii), wherein said biologically active analog consists of the same number of amino acids as (i), (ii), (iii), (iv), (v), (vi), or (vii), respectively, and has at least 70% sequence homology thereto; and (ix) a biologically active analog of (i), (ii), (iii), (iv), (v), (vi), (vii) or (viii), 35 wherein said analog consists of the amino acid sequence of (i), (ii), (iii), (iv), (v), (vi), (vii) or (viii), respectively, and an additional N-terminal methionine, 9 wherein said KGF polypeptide exhibits an increase in bioactivity relative to mature, full-length, KGF (KGFi 63 ) as determined by the Balb/MK bioactivity assay and further wherein said KGF polypeptide is present in a therapeutically effective amount 5 and the therapeutically effective amount is 75% or less of the amount on a per molecule basis of KGF 16 3 needed to elicit an equivalent therapeutic response. In yet further embodiments, the invention is directed to a composition comprising: (a) a therapeutically effective amount of a KGF polypeptide, wherein the 10 KGF polypeptide is selected from the group consisting of: (i) KGFdesii5 consisting of the contiguous amino acid sequence depicted at amino acid residues 16-163, inclusive, of Figure 1; (ii) KGFdesi.18 consisting of the contiguous amino acid sequence depicted at amino acid residues 19-163, inclusive, of Figure 1; 15 (iii) KGFdesi.19 consisting of the contiguous amino acid sequence depicted at amino acid residues 20-163, inclusive, of Figure 1; (iv) KGFdesi-20 consisting of the contiguous amino acid sequence depicted at amino acid residues 21-163, inclusive, of Figure 1; (v) KGFdesi-21 consisting of the contiguous amino acid sequence 20 depicted at amino acid residues 22-163, inclusive, of Figure 1; (vi) KGFes1.22 consisting of the contiguous amino acid sequence depicted at amino acid residues 23-163, inclusive, of Figure 1; (vii) KGFdesi-24 consisting of the contiguous amino acid sequence depicted at amino acid residues 25-163, inclusive, of Figure 1; 25 (viii) KGFes1-25 consisting of the contiguous amino acid sequence depicted at amino acid residues 26-163, inclusive, of Figure 1; (ix) a biologically active analog of (i), (ii), (iii), (iv), (v), (vi), (vii) or (viii), wherein said biologically active analog consists of the same number of amino acids as (i), (ii), (iii), (iv), (v), (vi), (vii) or (viii), respectively, and has at least 70% 30 sequence homology thereto; and (x) an analog of (i), (ii), (iii), (iv), (v), (vi), (vii), (viii) or (ix), consisting of the amino acid sequence of (i), (ii), (iii), (iv), (v), (vi), (vii), (viii) or (ix), respectively, and an additional N-terminal methionine, wherein the KGF polypeptide exhibits an increase in bioactivity relative to 35 mature, full-length, KGF (KGF1 63 ) as determined by the Balb/MK bioactivity assay and 10 specifically stimulates epithelial cell proliferation, and further wherein the therapeutically effective amount is 75% or less of the amount on a per molecule basis of KGF 1 63 needed to elicit an equivalent therapeutic response; and (b) a pharmaceutically acceptable excipient. 5 In certain embodiments, the biologically active analog has at least 80% sequence homology or at least 90% sequence homology to (i), (ii), (iii), (iv), (v), (vi), (vii), (viii) or (ix). These and other aspects of the present invention will become evident upon reference to the following detailed description and attached figures. In addition, 10 various references are set forth herein which describe in more detail certain procedures or compositions. Throughout this specification the word "comprise", or variations such as "comprises" or "comprising", will be understood to imply the inclusion of a stated element, integer or step, or group of elements, integers or steps, but not the exclusion of 15 any other element, integer or step, or group of elements, integers or steps. Brief Description of the Figures Figure 1 (SEQ ID NOS:25 and 26) depicts the DNA sequence and corresponding amino acid sequence for mature, full-length KGF (KGFi 6 3 ). 20 Figures 2A and 2B show a comparison of the biological activity of various N terminally truncated KGF polypeptides. Figure 2A compares activity of KGFdesi- 22 (*), KGFdesI- 23 (U), KGFdesi- 24 (A) KGFdesl- 26 (X) and KGFdesI-30 (double X), while Figure 2B shows a comparison of KGFdesi-23 (0), KGFdesI-26 (X) and KGFesi.30 (double X) with acidic FGF (aFGF, *, middle line) and full-length KGF (FL-KGF, (*, second 25 to the top line). 10A Figure 3 shows the results of experiments where various truncated KGF molecules were tested on vascular endothelial cells derived from either the bovine aortic arch (adult bovine aortic endothelial cells (ABAE), left side of figure) or from the bovine adrenal gland capillaries (adrenal cortex-derived capillary endothelial cells (ACE), right 5 side of figure). The histograms show the final cell density of cultures exposed to saturating concentrations of the various KGF polypeptides, or basic FGF (bFGF), after seven days in culture. Figure 4 shows the amount of soluble KGFeSI.24 (A) and KGFdee, (, determined by SDS-PAGE as a function of time of incubation at 37"C. 10 Figure 5 shows the amount of soluble KGFcsi-23 (U) and native KGF (FL, *) determined by SDS-PAGE as a function of time of incubation at 37"C. Figure 6 depicts the results of the thermal and acid stability test described in the examples. FL-KGF represents KGF,,,. T-KGF represents KGFel- 23 . The histograms represent the final cell density of the cultures after seven days when exposed to saturating 15 concentrations of either KGFesi-23 or KGF, 63 . Figure 7 shows the effect of increasing concentrations of native KGF (FL, *) and KGFe,,.IS (0), KGFeSI-23 (E) and KGFcsi.

2 5 (A) on the proliferation of Balb/Mk cells when added only once. Figure 8 (SEQ ID NOS:27 and 28) shows the DNA sequence and corresponding 20 amino acid sequence for KGFcsi- 22 , with the N-terminal arginine residue substituted with an alanine residue. Detailed Description of the Invention The practice of the present invention will employ, unless otherwise indicated, 25 conventional methods of protein chemistry, biochemistry, recombinant DNA techniques and pharmacology, within the skill of the art. Such techniques are explained fully in the literature. See, e.g., T.E. Creighton, Proteins: Structures and Molecular Properties (W.H. Freeman and Company, 1993); A.L. Lehninger, Biochemistry (Worth Publishers, Inc., current addition); Sambrook, et al., Molecular Cloning: A Laboratory Manual (2nd 30 Edition, 1989); Methods In Enzymology (S. Colowick and N. Kaplan eds., Academic 11 Press, Inc.); Remington's Pharmaceutical Sciences, 18th Edition (Easton, Pennsylvania: Mack Publishing Company, 1990). The following amino acid abbreviations are used throughout the text: Alanine: Ala (A) Arginine: Mg (R) 5 Asparagine: Asn (N) Aspartic acid: Asp (D) Cysteine: Cys (C) Glutamine: Gln (Q) Glutamic acid: Glu (E) Glycine: Gly (G) Histidine: His (H) Isoleucine: le (1) Leucine: Leu (L) Lysine: Lys (K) 10 Methionine: Met (M) Phenylalanine: Phe (F) Proline: Pro (P) Serine: Ser (S) Threonine: Thr (T) Tryptophan: Trp (W) Tyrosine: Tyr (Y) Valine: Val (V) 15 I. Definitions In describing the present invention, the following terms will be employed, and are intended to be defined as indicated below. The terms polypeptidee" and "protein" refer to a polymer of amino acid residues and are not limited to a minimum length of the product. The terms also include, unless 20 other-wise indicated, modifications of the polypeptide that do not change the sequence of amino acids, for example, glycosylated, acetylated and phosphorylated fors. A polypeptide or protein, for purposes of the present invention, may be synthetically or recombinantly produced, as well as isolated from natural sources. As used herein, the term keratinocytee growth factor" or aiKGF" refers to a 25 member of a group of the FGF family of proteins which is capable of binding to FGFR-2, lacks significant activity on fibroblasts, is uniquely specific for epithelial cells and is particularly active on keratinocytes. KGF, analogs and fragments thereof (defined below) may be synthetically or recombinantly produced. Moreover, KGF may be isolated from natural sources, such as from any of several tissues of any mammalian source, for 30 example from human tissues. 12 "Mature, full-length KGF," "long form of KGF," "FL-KGF," "native KGF" or

"KGF

163 " as used herein all refer to the mature polypeptide that contains 163 amino acid residues, as shown in Figure 1. 5 As used herein, the term "KGF fragment" refers to a polypeptide derived from

KGF,

6 3 that does not include the entire sequence of KGF 16 3 . Such a fragment may be a truncated version of the full-length molecule, as well as an internally deleted polypeptide. A KGF fragment may have KGF bioactivity as determined by the Balb/MK bioactivity assay, described in Example 4 herein. The Balb/MK cell line (Weissman, B. E. and 10 Aaronson, S. A. Cell (1983) .2:599-606) is a clonal Balb/c mouse keratinocyte cell line. These cells are dependent for their growth upon an exogenous source of an epithelial cell mitogen even in medium containing serum. Thus, activity of the KGF fragments and analogs is measured by determining the ED,, value using Balb/Mk cells, said value defined by the concentration of KGF fragment that causes half maximal stimulation of 15 cell proliferation. Additionally, the KGF fragments of the invention specifically stimulate epithelial cell proliferation. To determine target cell specificity, DNA synthesis stimulation, expressed as the ratio of stimulated synthesis over background incorporation of thymidine in the absence of added test sample, is compared to analogous stimulation observed in cells other than 20 keratinocytes under the same assay conditions. The activity of the KGF fragments and analogs can also be tested on endothelial cells, such as adult bovine aortic endothelial cells (ABAE) or adrenal cortex-derived capillary endothelial cells (ACE), as described in Example 5 herein. A KGF polypeptide or analog that "specifically stimulates epithelial cell proliferation" may be a molecule that, at saturating concentrations, (i) in the Balb/Mk 25 assay described in Example 4 herein, can stimulate the final cell number per well after 7 days in culture to a level at least 4-fold higher than the cell number achieved in wells receiving no KGF; and (ii) in the ABAE or ACE assay described in Example 5 herein, does not significantly stimulate the final cell number per well after 7 days in culture to a level higher than the cell number achieved in wells receiving no KGF. 13 U.S. Patent No. 5,731,170, incorporated by reference herein in its entirety, reports that certain molecules display KGF mitogenic activity with marked specificity for keratinocytes as opposed to fibroblasts. The fragments of the present invention will display enhanced activity on a per 5 molecule basis relative to KGF,3' such as anywhere from 10% or more activity, such as 15%, 20%, 25%, 50%, 100% or more, to as much as 10-fold or more activity, or any amount between the specified ranges. Hence, the KGF fragments of the present invention may be used in compositions in lesser amounts than would be necessary using KGF,,,. The inventors herein recognize that truncations produce molecules of lower molecular 10 weight than full-length KGF. As shown below in the Examples, these species are more active when compared on a per molecule basis (i.e., when activity is adjusted for the molecular weight). Particular KGF fragments are described in detail below. The term "analog" refers to derivatives of the reference molecule. The analog may retain biological activity, as described above. In general, the term "analog" refers to 15 compounds having a native polypeptide sequence and structure with one or more amino acid additions, substitutions (generally conservative in nature) and/or deletions, relative to the native molecule, so long as the modifications do not destroy activity. Preferably, the analog has at least the same biological activity as the parent molecule, and may even display enhanced activity over the parent molecule. Methods for making polypeptide 20 analogs are known in the art and are described further below. Particularly preferred analogs include substitutions that are conservative in nature, i.e., those substitutions that take place within a family of amino acids that are related in their side chains. Specifically, amino acids are generally divided into four families: (1) acidic -- aspartate and glutamate; (2) basic -- lysine, arginine, histidine; (3) non-polar - 25 alanine, valine, leucine, isoleucine, proline, phenylalanine, methionine, tryptophan; and (4) uncharged polar -- glycine, asparagine, glutamine, cysteine, seine, threonine, tyrosine. Phenylalanine, tryptophan, and tyrosine are sometimes classified as aromatic amino acids. For example, it is reasonably predictable that an isolated replacement of leucine with isoleucine or valine, an aspartate with a glutamate, a threonine with a seine, 30 or a similar conservative replacement of an amino acid with a structurally related amino 14 acid, will not have a major effect on the biological activity. For example, the polypeptide of interest may include up to about 1-70 conservative or non-conservative amino acid substitutions, such as 1, 2, 3, 4, 5-50, 15-25, 5-10, or any integer between 1-70, so long as the desired function of the molecule remains intact. One of skill in the art may readily 5 determine regions of the molecule of interest that can be modified with a reasonable likelihood of retaining biological activity as defined herein. For example, none of the critical determinants involved in signaling appear to be located within the first 30 N-terminal amino acids of KGF (Plotnikov, et. al. Cell (2000) 101:413-424). Additionally, the NH 2 terminal domain of KGF does not appear to be 10 involved in its cell specificity. Amino acid residues 91-110, numbered relative to the amino acid sequence set forth in Figure 1 appear to confer receptor binding specificity to KGF (Reich-Slotsky, et al. J. Biol. Chem. (1995) 20:29813-29818). Thus, analogs and fragments which retain the region spanning at least amino acids 91-110 are preferred. Moreover, if amino acid substitutions are made in this region, they should be 15 conservative in nature. Fragments which retain portions of the N-terminal sequence, e.g., fragments with deletions that do not extend to, for example, amino acid 35, numbered relative to Figure 1, are more tolerable to amino acid additions, deletions and substitutions. Preferred deletions include deletions of the first 22, 23 and 24 amino acids, as described further below. One of skill in the art can readily determine other regions that 20 will tolerate change based on the known structure of KGF (see, e.g., Osslund et al. Protein Sci. (1998) 7:1681-1690), as well as the known structure/function relationships between KGF and related molecules such as acidic FGF, basic FGF and kaposi FGF (see, e.g., Gospodarowicz et al., J Cell. Physiol. (1990) 142:325-333). By "purified" and "isolated" is meant, when referring to a polypeptide or 25 polynucleotide, that the indicated molecule is present in the substantial absence of other biological macromolecules of the same type. The term "purified" as used herein preferably means at least 75% by weight, more preferably at least 85% by weight, more preferably still at least 95% by weight, and most preferably at least 98% by weight, of biological macromolecules of the same type are present in the sample. An "isolated 30 polynucleotide which encodes a particular polypeptide" refers to a nucleic acid molecule 15 which is substantially free of other nucleic acid molecules that do not encode the subject polypeptide; however, the molecule may include some additional bases or moieties which do not deleteriously affect the basic characteristics of the composition. By a "recombinant polypeptide" is intended a polypeptide which has been 5 prepared by recombinant DNA techniques as described herein. In general, the gene coding for the desired polypeptide is cloned and then expressed in transformed organisms, as described further below. The host organism expresses the foreign gene to produce the polypeptide under expression conditions. Alternatively, the promoter controlling expression of an endogenous polypeptide can be altered to render a 10 recombinant polypeptide. It is particularly advantageous to produce polypeptides recombinantly as recombinant production generally allows for higher yields from less starting material, and renders a far purer product. Thus, the polypeptides of the invention can be produced in the absence of other molecules normally present in cells. For example, human polypeptide compositions free of any trace of human protein 15 contaminants can be readily obtained because the only human protein produced by a recombinant non-human host cell is the recombinant human polypeptide. Potential viral agents from natural sources and viral components pathogenic to humans are also avoided. The term "polynucleotide" or "nucleic acid molecule" as used herein refers to a polymeric form of nucleotides of any length, either ribonucleotides or 20 deoxyribonucleotides. This term refers only to the primary structure of the molecule and thus includes double- and single-stranded DNA and RNA. It also includes known types of modifications, for example, labels which are known in the art, methylation, "caps", substitution of one or more of the naturally occurring nucleotides with an analog, internucleotide modifications such as, for example, those with uncharged linkages (e.g., 25 methyl phosphonates, phosphotriesters, phosphoamidates, carbamates, etc.) and with charged linkages (e.g., phosphorothioates, phosphorodithioates, etc.), those containing pendant moieties, such as, for example proteins (including for e.g., nucleases, toxins, antibodies, signal peptides, poly-L-lysine, etc.), those with intercalators (e.g., acridine, psoralen, etc.), those containing chelates (e.g., metals, radioactive metals, boron, oxida 16 tive metals, etc.), those containing alkylators, those with modified linkages (e.g., alpha anomeric nucleic acids, etc.), as well as unmodified forms of the polynucleotide. The terms "recombinant DNA molecule," or "recombinant polynucleotide" are used herein to refer to a polynucleotide of genomic, cDNA, semisynthetic, or synthetic 5 origin which, by virtue of its origin or manipulation: (1) is not associated with all or a portion of a polynucleotide with which it is associated in nature, (2) is linked to a polynucleotide other than that to which it is linked in nature, or (3) does not occur in nature. Thus, the term encompasses "synthetically derived" nucleic acid molecules. A "coding sequence" is a nucleic acid molecule which is translated into a 10 polypeptide, usually via mRNA, when placed under the control of appropriate regulatory sequences. The boundaries of the coding sequence may be determined by a translation start codon at the 5'-terminus and a translation stop codon at the 3'-terminus. A coding sequence can include, but is not limited to, cDNA, and recombinant nucleotide sequences. 15 "Control sequences" refer to nucleic acid sequences which are necessary to effect the expression of coding sequences to which they are ligated. The nature of such control sequences differs depending upon the host organism; in prokaryotes, such control sequences generally include promoter, ribosomal binding site, and transcription termination sequence; in eukaryotes, generally, such control sequences include promoters 20 and transcription tennination sequences. The term "control sequences" is intended to include, at a minimum, all components necessary for expression of a coding sequence, and may also include additional components, for example, leader sequences and fusion partner sequences. A control element, such as a promoter, "directs the transcription" of a coding 25 sequence in a cell when RNA polymerase will bind the promoter and transcribe the coding sequence into mRNA, which is then translated into the polypeptide encoded by the coding sequence. "Operably linked" refers to a juxtaposition wherein the components so described are in a relationship permitting them to function in their intended manner. A control 30 sequence "operably linked" to a coding sequence is ligated in such a way that expression 17 of the coding sequence is achieved under conditions compatible with the control sequences. The control elements need not be contiguous with the coding sequence, so long as they function to direct the expression thereof. Thus, for example, intervening untranslated yet transcribed sequences can be present between a promoter and the coding 5 sequence and the promoter can still be considered "operably linked" to the coding sequence. As used herein, the term "expression cassette" refers to a molecule comprising at least one coding sequence operably linked to a control sequence which includes all nucleotide sequences required for the transcription of cloned copies of the coding 10 sequence and the translation of the mRNAs in an appropriate host cell. Such expression cassettes can be used to express eukaryotic genes in a variety of hosts such as bacteria, blue-green algae, plant cells, yeast cells, insect cells and animal cells. Under the invention, expression cassettes can include, but are not limited to, cloning vectors, specifically designed plasmids, viruses or virus particles. The cassettes may further 15 include an origin of replication for autonomous replication in host cells, selectable markers, various restriction sites, a potential for high copy number and strong promoters. By "vector" is meant any genetic element, such as a plasmid, phage, transposon, cosmid, chromosome, virus etc., which is capable of replication when associated with the proper control elements and which can transfer gene sequences between cells. Thus, the 20 term includes cloning and expression vehicles, as well as viral vectors. A cell has been "transformed" by an exogenous polynucleotide when the polynucleotide has been introduced inside the cell membrane. The exogenous polynucleotide may or may not be integrated (covalently linked) into chromosomal DNA making up the genome of the cell. In procaryotes and yeasts, for example, the exogenous 25 DNA may be maintained on an episomal element, such as a plasmid. With respect to eucaryotic cells, a stably transformed cell is one in which the exogenous DNA has become integrated into the chromosome so that it is inherited by daughter cells through chromosome replication. This stability is demonstrated by the ability of the eucaryotic cell to establish cell lines or clones comprised of a population of daughter cells containing 30 the exogenous DNA. 18 A "host cell" is a cell which has been transformed, or is capable of transformation, by an exogenous nucleic acid molecule. "Homology" refers to the percent similarity between two polynucleotide or two polypeptide moieties. Two DNA, or two polypeptide sequences are "substantially 5 homologous" to each other when the sequences exhibit at least about 50%, preferably at least about 70% to 75%, more preferably at least about 80%-85%, preferably at least about 90%, and most preferably at least about 95%-98% sequence homology, or any percent homology between the specified ranges, over a defined length of the molecules. As used herein, substantially homologous also refers to sequences showing complete identity to the 10 specified DNA or polypeptide sequence. In general, "identity" refers to an exact nucleotide-to-nucleotide or amino acid-to amino acid correspondence of two polynucleotides or polypeptide sequences, respectively. Percent identity can be determined by a direct comparison of the sequence information between two molecules by aligning the sequences, counting the exact number of matches 15 between the two aligned sequences, dividing by the length of the shorter sequence, and multiplying the result by 100. Preferably, naturally or non-naturally occurring protein variants have amino acid sequences which are at least 70%, 80%, 85%, 90% or 95% or more homologous to the particular KGF fragment derived from Figure 1. More preferably, the molecules are 98% 20 or 99% homologous. Percent homology is determined using the Smith-Waterman homology search algorithm using an affine gap search with a gap open penalty of 12 and a gap extension penalty of 2, BLOSUM matrix of 62. The Smith-Waterman homology search algorithm is taught in Smith and Waterman, Adv. Apple. Math. 2:482-489 (1981). Alternatively, homology can be determined by hybridization of polynucleotides 25 under conditions which form stable duplexes between homologous regions, followed by digestion with single-stranded-specific nuclease(s), and size determination of the digested fragments. DNA sequences that are substantially homologous can be identified in a Southem hybridization experiment under, for example, stringent conditions, as defined for that particular system. Defining appropriate hybridization conditions is within the skill of 19 the art. See, e.g., Sambrook et al., supra; DNA Cloning, supra; Nucleic Acid Hybridization, supra. The terms "effective amount" or "pharmaceutically effective amount" refer to a nontoxic but sufficient amount of the agent to provide the desired biological result. That 5 result can be reduction and/or alleviation of the signs, symptoms, or causes of a disease, or any other desired alteration of a biological system. For example, an effective amount of a KGF fragment for use with the present methods is an amount sufficient to stimulate epithelial cell stimulation or proliferation, and preferably an amount sufficient to cause increased healing of wounds and/or bums, and other disorders where epithelial cell 10 proliferation is desired. Such amounts are described below. An appropriate "effective" amount in any individual case may be determined by one of ordinary skill in the art using routine experimentation. By "pharmaceutically acceptable" or "pharmacologically acceptable" is meant a material which is not biologically or otherwise undesirable, i.e., the material may be 15 administered to an individual without causing any undesirable biological effects or interacting in a deleterious manner with any of the components of the composition in which it is contained. By "physiological pH" or a "pH in the physiological range" is meant a pH in the range of approximately 7.0 to 8.0 inclusive. Preferred physiological pH is in the range of 20 approximately 7.2 to 7.6 inclusive. As used herein, the term "subject" encompasses mammals and non-mammals. Examples of mammals include, but are not limited to, any member of the Mammalia class: humans, non-human primates such as chimpanzees, and other apes and monkey species; farm animals such as cattle, horses, sheep, goats, swine; domestic animals such as rabbits, 25 dogs, and cats; laboratory animals including rodents, such as rats, mice and guinea pigs, and the like. Examples of non-mammals include, but are not limited to, birds, fish and the like. The term does not denote a particular age or gender. 20 II. Modes of Carrying Out the Invention Before describing the present invention in detail, it is to be understood that this invention is not limited to particular formulations or process parameters as such may, of course, vary. It is also to be understood that the terminology used herein is for the purpose 5 of describing particular embodiments of the invention only, and is not intended to be limiting. Although a number of compositions and methods similar or equivalent to those described herein can be used in the practice of the present invention, the preferred materials and methods are described herein. 10 The present invention is based on the discovery that certain KGF polypeptide fragments and analogs of these fragments, which retain only a portion of the full-length sequence, show enhanced bioactivity relative to the full-length sequence. Thus, smaller amounts of the polypeptide may be used in compositions than would be necessary with the full-length molecule. In certain instances, there is less occurrence of non-specific side 15 effects when compositions including the molecules described herein are administered. The present invention particularly relates to compositions comprising KGF fragments which exhibit an increase in bioactivity relative to KGF, 6 , as determined by the Balb/MK bioactivity assay specified herein and which specifically stimulate epithelial cell proliferation. Particularly, activity of the KGF fragments and analogs is measured by 20 determining the ED 50 value using Balb/Mk cells, said value defined by the concentration of KGF fragment that causes half maximal stimulation of cell proliferation. The cells are cultured for 7 days, as described below in the examples. The bioactivity of the KGF fragments herein is preferably at least about 1.2 to 1.5-fold, preferably, about 2-fold and, more preferably, about 2- to 10-fold greater or more than that of the full-length KGF 25 protein, when compared in the cell proliferation assay, and may be as much as 10- to 100-fold greater or more than full-length KGF, as determined using stimulation of DNA synthesis in Balb/Mk cells maintained in a chemically defined medium, as described herein and in PCT Patent Application, No. WO 90/08771. Due to the enhanced activity level, the polypeptides of the subject invention allow the use of 90% or less, such as 5% 30 90%, or 10%-50%, or any number therebetween, on a per molecule basis (i.e., adjusted for 21 the molecular weight) of the amount of KGF 63 that would be necessary in corresponding compositions to achieve the same biological results. Particular polypeptides for use herein include but are not limited to the following: KGFd.., consisting of the contiguous amino acid sequence depicted at amino acid 5 residues 16-163, inclusive, of Figure 1; an analog of KGF,,, .1 consisting of the contiguous amino acid sequence depicted at amino acid residues 16-163, inclusive, of Figure 1 and having an additional N-terminal methionine; KGFde,., consisting of the contiguous amino acid sequence depicted at amino acid residues 19-163, inclusive, of Figure 1; an analog of KGFde,,.I consisting of the 10 contiguous amino acid sequence depicted at amino acid residues 19-163, inclusive, of Figure 1 and having an additional N-terminal methionine; KGF,,-I, consisting of the contiguous amino acid sequence depicted at amino acid residues 20-163, inclusive, of Figure 1; an analog KGFCI-, consisting of the contiguous amino acid sequence depicted at amino acid residues 20-163, inclusive, of Figure 1 with 15 an additional N-terminal methionine; KGFde,-20 consisting of the contiguous amino acid sequence depicted at amino acid residues 21-163, inclusive, of Figure 1; an analog of KGFd,,-20 consisting of the contiguous amino acid sequence depicted at amino acid residues 21-163, inclusive, of Figure 1 with an additional N-terminal methionine; 20 KGF,,S- 2 I consisting of the contiguous amino acid sequence depicted at amino acid residues 22-163, inclusive, of Figure 1; an analog of KGFeSl-21 consisting of the contiguous amino acid sequence depicted at amino acid residues 22-163, inclusive, of Figure 1 with an additional N-terminal methionine; KGFdI- 2 2 consisting of the contiguous amino acid sequence depicted at amino acid 25 residues 23-163, inclusive, of Figure 1; an analog of KGFd, 2 Z consisting of the contiguous amino acid sequence depicted at amino acid residues 23-163, inclusive, of Figure 1 with an additional N-terminal methionine; an analog of KGF,,1- 22 consisting of the contiguous amino acid sequence depicted at amino acid residues 23-163, inclusive, of Figure 1 with the N-terminal arginine residue substituted with an alanine residue; 22 KGFesi-24 consisting of the contiguous amino acid sequence depicted at amino acid residues 25-163, inclusive, of Figure 1; an analog of KGFesI.

24 consisting of the contiguous amino acid sequence depicted at amino acid residues 25-163, inclusive, of Figure 1 with an additional N-terminal methionine; and 5 KGFesI- 2 5 consisting of the contiguous amino acid sequence depicted at amino acid residues 26-163, inclusive, of Figure 1; and an analog of KGFesI-2S consisting of the contiguous amino acid sequence depicted at amino acid residues 26-163, inclusive, of Figure 1 with an additional N-terminal methionine. Also contemplated for use in the subject compositions are biologically active 10 analogs of the above-specified fragments, wherein the biologically active analogs consist of the same number of amino acids as the above fragments and have at least about 50%, preferably at least about 70%, preferably at least about 75%, preferably at least about 80%, preferably at least about 85%, preferably at least about 90%, preferably at least about 95%, and preferably at least about 98% sequence homology thereto, as determined as described 15 above. For example, the biologically active analog may be an analog of KGFS., 15 that consists of 148 amino acids and has at least 70% sequence homology thereto; an analog of KGFe,S- that consists of 145 amino acids and has at least 70% sequence homology thereto; an analog of KGFd.e_ 9 that consists of 144 amino acids and has at least 70% sequence homology thereto; an analog of KGFes- 2 0 that consists of 143 amino acids and 20 has at least 70% sequence homology thereto; an analog of KGFde,-21 that consists of 142 amino acids and has at least 70% sequence homology thereto; an analog of KGFS.

22 that consists of 141 amino acids and has at least 70% sequence homology thereto; an analog of KGFe- 24 that consists of 139 amino acids and has at least 70% sequence homology thereto; and an analog of KGFdcs-2 5 that consists of 138 amino acids and has at least 70% 25 sequence homology thereto. The amount of KGF polypeptide for use in the subject compositions relative to KGF, will vary depending on the particular fragment of interest. In general, compositions will comprise about 90%, or less, even 75%, or less, 50%, or less, 35%, or less, 25%, or less, or 10% or less, on a per molecule basis (i.e., adjusted for molecular 30 weight), of the amount of KGF, 1 3 in a corresponding composition that would be necessary 23 to achieve the desired result, such as to promote epithelial cell division and/or proliferation. Thus, for example, the compositions described herein may include 5%-90%, or 10%-90%, or 10%-75%, or 10%-50%, or 10%-25%, or 10%-20% on a per molecule basis, of the amount of KGF,, in a corresponding composition that would be necessary to 5 achieve the desired result. It is to be understood that particular percentages between these ranges are also contemplated herein. Particularly, if the KGF fragment is KGFe,,_,,, KGF.,,_ M, KGFe,,_,, KGFdesl.,2, KGFcs 2 ,, KGFdcsi-22 KGFdcsi-24 KGFdcsl-2s, or polypeptides derived from these molecules, the amount may be 75%, or less, such as 10% to 75%. If the KGF fragment is KGFdeeI- 22 10 or KGFei- 24 , or polypeptides derived from these molecules, the amount used may 50%, or less, or even 25%, or 20%, or less, such as 5% to 50%. For KGFde0 2 4 and polypeptides derived therefrom, for example, the amount may be 10%, or less, such as 2% to 10% of the amount required of KGFi 1 6 to achieve an equivalent therapeutic response. Appropriate amounts are discussed in detail below. 15 In a preferred embodiment of the present invention, the KGF fragments of the present invention.are produced by recombinant technology, particularly in the case of large-scale commercial production. Recombinant DNA molecules and expression vectors encoding the polypeptides of the present invention can be made and the genes expressed by conventional gene expression technology using methods well-known in the art, as 20 discussed in more detail below. Analogs of particular KGF fragments may also be made recombinantly, for example, by site-directed mutagenesis. Thus, all references to embodiments of the present invention as they relate to particular KGF fragments apply equally to analogs of the fragments. In one embodiment of the present invention, the KGF fragment can be made by 25 isolating native, mature KGF from cells producing the same, such as M426 human embryonic fibroblasts (Aaronson, S. A. and Todaro, G. J. Virology (1968) 36:254-261), using techniques described in, e.g., U.S. Patent No. 5,731,170. N-terminal amino acid residues can then be deleted from the recovered molecule. Such deletion can be performed by any conventional techniques known in the art. 24 Alternatively, polypeptides for use in the subject compositions can be synthesized chemically, by any of several techniques that are known to those skilled in the peptide art. See, e.g., J. M. Stewart and J. D. Young, Solid Phase Peptide Synthesis (Pierce Chemical Co., Rockford, IL 1984) and G. Barany and R. B. Merrifield, The Peptides: Analysis, 5 Synthesis, Biology, editors E. Gross and J. Meienhofer, Vol. 2, (Academic Press, New York, 1980), pp. 3-254, for solid phase peptide synthesis techniques; and M. Bodansky, Principles ofPeptide Synthesis, (Springer-Verlag, Berlin 1984) and E. Gross and J. Meienhofer, Eds., The Peptides: Analysis, Synthesis, Biology, Vol. 1, for classical solution synthesis. The polypeptides of the present invention can also be chemically prepared by 10 the method of simultaneous multiple peptide synthesis. See, e.g., Houghten Proc. Natl. Acad. Sci. USA (1985) 82:5131-5135; U.S. Patent No. 4,631,211. In an alternative embodiment, the KGF fragments can be made by isolating the coding sequence of native KGF 1 63 , deleting the codons that encode the amino acid residues to be deleted, inserting the modified coding sequence into an expression vector, 15 transforming host cells with the expression vector to produce the recombinant KGF fragments and analogs, and isolating the recombinant KGF fragment using conventional purification techniques. In a further embodiment of the present invention, the coding sequence of the KGF fragment can be obtained by conventional techniques, including the isolation of the coding 20 sequence of KGF 163 from a cDNA library known to contain such, and deleting therefrom the sequence encoding the portion of amino acid residues to be deleted. Deletion of the coding sequence of the N-terminal amino acids can be accomplished in vivo or in vitro. The former can be achieved, for example, by expression of the KGF, 63 coding sequence in an appropriate expression system. The latter can be achieved by known PCR techniques 25 using primers that exclude the N-terminal sequences. Preferably, polypeptides for use in the present compositions are produced recombinantly, by expression of a polynucleotide encoding the same. Methods for the recombinant production of KGF fragments are well known. See, e.g., U.S. Patent Nos. 5,677,278, 5,773,586, 5,843,883, 5,863,767 and 6,074,848, all to Gospodarowicz et al.; 25 International Publications WO 96/11951 and WO 96/11949; and Osslund et al. Protein Sci. (1998) 7:1681-1690. In particular, the molecules for use with the present invention can be made using standard techniques of molecular biology. For example, polynucleotide sequences coding 5 for the above-described molecules can be obtained using recombinant methods, such as by screening cDNA and genomic libraries from cells expressing the gene, or by deriving the gene from a vector known to include the same. Furthermore, the desired gene can be isolated directly from cells and tissues containing the same, using standard techniques, such as phenol extraction and PCR of cDNA or genomic DNA. See, e.g., Sambrook et al., 10 supra, for a description of techniques used to obtain and isolate DNA. The gene of interest can also be produced synthetically, rather than cloned. The molecules can be designed with appropriate codons for the particular sequence. The complete sequence is then assembled from overlapping oligonucleotides prepared by standard methods and as sembled into a complete coding sequence. See, e.g., Edge (1981) Nature 292:756; 15 Nambair et al. (1984) Science 223:1299; and Jay et al. (1984) J. Biol. Chem. 259:63 11. Thus, particular nucleotide sequences can be obtained from vectors harboring the desired sequences or synthesized completely or in part using various oligonucleotide synthesis techniques known in the art, such as site-directed mutagenesis and polymerase chain reaction (PCR) techniques where appropriate. See, e.g., Sambrook, supra. In 20 particular, one method of obtaining nucleotide sequences encoding the desired sequences is by annealing complementary sets of overlapping synthetic oligonucleotides produced in a conventional, automated polynucleotide synthesizer, followed by ligation with an appropriate DNA ligase and amplification of the ligated nucleotide sequence via PCR. See, e.g., Jayaraman et al. (1991) Proc. Natl. Acad. Sci. USA 88:4084-4088. Additionally, 25 oligonucleotide directed synthesis (Jones et al. (1986) Nature 54:75-82), oligonucleotide directed mutagenesis of pre-existing nucleotide regions (Riechmann et al. (1988) Nature 332:323-327 and Verhoeyen et al. (1988) Science 239:1534-1536), and enzymatic filling in of gapped oligonucleotides using T 4 DNA polymerase (Queen et al. (1989) Proc. NatI. Acad. Sci. USA 86:10029-10033) can be used under the invention to provide molecules 30 having altered or enhanced receptor-binding capabilities, and/or reduced immunogenicity. 26 Once coding sequences have been prepared or isolated, such sequences can be cloned into any suitable vector or replicon. Numerous cloning vectors are known to those of skill in the art, and the selection of an appropriate cloning vector is a matter of choice. Suitable vectors include, but are not limited to, plasmids, phages, transposons, cosmids, 5 chromosomes or viruses which are capable of replication when associated with the proper control elements. The coding sequence is then placed under the control of suitable control elements, depending on the system to be used for expression. Thus, the coding sequence can be placed under the control of a promoter, ribosome binding site (for bacterial expression) 10 and, optionally, an operator, so that the DNA sequence of interest is transcribed into RNA by a suitable transformant. The coding sequence may or may not contain a sequence coding for a signal peptide or leader sequence which can later be removed by the host in post-translational processing. See, e.g., U.S. Patent Nos. 4,431,739; 4,425,437; 4,338,397. If a signal sequence is present, it can either be the native sequence or it may be a 15 heterologous signal sequence. In addition to control sequences, it may be desirable to add regulatory sequences which allow for regulation of the expression of the sequences relative to the growth of the host cell. Regulatory sequences are known to those of skill in the art, and examples include those which cause the expression of a gene to be turned on or off in response to a 20 chemical or physical stimulus, including the presence of a regulatory compound. Other types of regulatory elements may also be present in the vector. For example, enhancer elements may be used herein to increase expression levels of the constructs. Examples include the SV40 early gene enhancer (Dijkema et al. (1985) EMBO J. 4:761), the enhancer/promoter derived from the long terminal repeat (LTR) of the Rous Sarcoma 25 Virus (Gorman et al. (1982) Proc. Nati. Acad. Sci. USA 79:6777) and elements derived from human CMV (Boshart et al. (1985) Cell 41:521), such as elements included in the CMV intron A sequence (U.S. Patent No. 5,688,688). The expression cassette may further include an origin of replication for autonomous replication in a suitable host cell, one or more selectable markers, one or more restriction sites, a potential for high copy number 30 and a strong promoter. 27 An expression vector is constructed so that the particular coding sequence is located in the vector with the appropriate regulatory sequences, the positioning and orientation of the coding sequence with respect to the control sequences being such that the coding sequence is transcribed under the "control" of the control sequences (i.e., RNA 5 polymerase which binds to the DNA molecule at the control sequences transcribes the coding sequence). Modification of the sequences encoding the molecule of interest may be desirable to achieve this end. For example, in some cases it may be necessary to modify the sequence so that it can be attached to the control sequences in the appropriate orientation; i.e., to maintain the reading frame. The control sequences and other 10 regulatory sequences may be ligated to the coding sequence prior to insertion into a vector. Alternatively, the coding sequence can be cloned directly into an expression vector which already contains the control sequences and an appropriate restriction site. As explained above, it may also be desirable to produce mutants or analogs of the reference KGF fragment. Mutants or analogs may be prepared by the deletion of a portion 15 of the sequence encoding the KGF fragment, by insertion of a sequence, and/or by substitution of one or more nucleotides within the sequence. Techniques for modifying nucleotide sequences, such as site-directed mutagenesis, and the like, are well known to those skilled in the art. See, e.g., Sambrook et al., supra; Kunkel, T.A. (1985) Proc. Natl. Acad. Sci. USA (1985) 2:448; Geisselsoder et al. (1987) BioTechniques 5:786; Zoller and 20 Smith (1983) Methods Enzymol. 100:468; Dalbie-McFarland et al. (1982) Proc. Natl. Acad. Sci USA 79:6409. The molecules can be expressed in a wide variety of systems, including insect, mammalian, bacterial, viral and yeast expression systems, all well known in the art. For example, insect cell expression systems, such as baculovirus systems, are known to those 25 of skill in the art and described in, e.g., Summers and Smith, Texas Agricultural Experiment Station Bulletin No. 1555 (1987). Materials and methods for baculovirus/insect cell expression systems are commercially available in kit form from, inter alia, Invitrogen, San Diego CA ("MaxBac" kit). Similarly, bacterial and mammalian cell expression systems are well known in the art and described in, e.g., Sambrook et al., 28 supra. Yeast expression systems are also known in the art and described in, e.g., Yeast Genetic Engineering (Barr et al., eds., 1989) Butterworths, London. A number of appropriate host cells for use with the above systems are also known. For example, mammalian cell lines are known in the art and include immortalized cell 5 lines available from the American Type Culture Collection (ATCC), such as, but not limited to, Chinese hamster ovary (CHO) cells, HeLa cells, baby hamster kidney (BHK) cells, monkey kidney cells (COS), human embryonic kidney cells, human hepatocellular carcinoma cells (e.g., Hep G2), Madin-Darby bovine kidney ("MDBK") cells, as well as others. Similarly, bacterial hosts such as E. coli, Bacillus subtilis, and Streptococcus spp., 10 will find use with the present expression constructs. Yeast hosts useful in the present invention include inter alia, Saccharonyces cerevisiae, Candida albicans, Candida maltosa, Hansenula polymorpha, Kluyveromycesfragilis, Kluyveromyces lactis,-Pichia guillerinondii, Pichia pastoris, Schizosaccharonzyces pombe and Yarrowia lipolytica. Insect cells for use with baculovirus expression vectors include, inter alia, Aedes aegypti, 15 Autographa californica, Bombyx mori, Drosophila melanogaster, Spodopterafrugiperda, and Trichoplusia ni. Intracellular expression of the truncated KGF polypeptides and analogs thereof in yeast is particularly desirable. Such systems avoid problems which may arise with purification from bacteria, such as E. coli, including the presence of large amounts of 20 DNA, endotoxins, and protein contaminants. Moreover, naturally occurring yeast enzymes efficiently cleave the N-terminal methionine which may be present when the molecules are recombinantly produced and there is no need to overexpress the enzyme when a yeast system is used. Additionally, although KGF is a naturally secreted protein, when native and truncated KGF, and analogs thereof, are produced internally in yeast 25 without secretion, they are soluble, properly folded and active. Nucleic acid molecules comprising nucleotide sequences of interest can be stably integrated into a host cell genome or maintained on a stable episomal element in a suitable host cell using various gene delivery techniques well known in the art. See, e.g., U.S. Patent No. 5,399,346. 29 Depending on the expression system and host selected, the molecules are produced by growing host cells transformed by an expression vector described abovc under conditions whereby the protein is expressed. The expressed protein is then isolated from the host cells and purified. If the expression system secretes the protein into growth 5 media, the product can be purified directly from the media. If it is not secreted, it can be isolated from cell lysates. The selection of the appropriate growth conditions and recovery methods are within the skill of the art. The KGF fragments are then formulated into pharmaceutical compositions, described further below, for delivery to a subject. Alternatively, polynucleotides encoding 10 the polypeptide of interest may be delivered directly to the subject and expressed in vivo. A number of viral based systems have been developed for direct gene transfer into mammalian cells. In this regard, retroviruses provide a convenient platform for gene delivery systems. A selected nucleotide sequence encoding the desired polypeptide can be inserted into a vector and packaged in retroviral particles using techniques known in the 15 art. The recombinant virus can then be isolated and delivered to a subject. A number of suitable retroviral systems have been described (U.S. Patent No. 5,219,740; Miller and Rosman (1989) Bio Techniques 7:980-990; Miller, A.D. (1990) Human Gene Therapy 1:5 14; Scarpa et al. (1991) Virology 180:849-852; Burns et al. (1993) Proc. Natl. Acad. Sci. USA 90:8033-8037; and Boris-Lawrie and Temin (1993) Cur. Opin. Genet. Develop. 20 3:102-109. A number of suitable adenovirus vectors have also been described. Unlike retroviruses which integrate into the host genome, adenoviruses persist extrachromosomally thus minimizing the risks associated with insertional mutagenesis (Haj-Ahmad and Graham (1986) J. Virol. 57:267-274; Bett et al. (1993) J. Virol. 67:5911 25 5921; Mittereder et al. (1994) Human Gene Therapy 5:717-729; Seth et al. (1994) J Virol. 68:933-940; Barr et al. (1994) Gene Therapy 1:51-58; Berkner, K.L. (1988) BioTechniques 6:616-629; and Rich et al. (1993) Human Gene Therapy 4:461-476). Various adeno-associated virus (AAV) vector systems have been developed recently for gene delivery. Such systems can include control sequences, such as promoter and 30 polyadenylation sites, as well as selectable markers or reporter genes, enhancer sequences, 30 and other control elements which allow for the induction of transcription. AAV vectors can be readily constructed using techniques well known in the art. See, e.g., U.S. Patent Nos. 5,173,414 and 5,139,941; International Publication Nos. WO 92/01070 (published 23 January 1992) and WO 93/03769 (published 4 March 1993); Lebkowski et al. (1988) 5 Molec. Cell. Biol. 8:3988-3996; Vincent et al. (1990) Vaccines 90 (Cold Spring Harbor Laboratory Press); Carter, B.J. (1992) Current Opinion in Biotechnology 3:533-539; Muzyczka, N. (1992) Current Topics in Microbiol. and Immunol. 158:97-129; Kotin, R.M. (1994) Human Gene Therapy 5:793-801; Shelling and Smith (1994) Gene Therapy 1:165-169; and Zhou et al. (1994) J Exp. Med. 179:1867-1875. 10 Additional viral vectors which will find use for delivering the nucleic acid molecules encoding the molecules of the present invention by gene transfer include those derived from the pox family of viruses, such as vaccinia virus and avian poxvirus. See, e.g., International Publication Nos. WO 91/12882; WO 89/03429; and WO 92/03545. Molecular conjugate vectors, such as the adenovirus chimeric vectors described in 15 Michael et al. (1993) J. Biol. Chem. 268:6866-6869 and Wagner et al. (1992) Proc. Natl. A cad. Sci. USA 89:6099-6103, can also be used for gene delivery under the invention. Members of the Alphavirus genus, such as but not limited to vectors derived from the Sindbis and Semliki Forest viruses, will also find use as viral vectors for delivering the gene of interest. For a description of Sinbus-virus derived vectors useful for the practice 20 of the instant methods, see, Dubensky et al., J Virol. (1996) 20:508-519; and International Publication Nos. WO 95/07995 and WO 96/17072. The gene of interest can also be delivered without a viral vector. For example, the gene can be packaged in liposomes prior to delivery to the subject or to cells derived therefrom, with or without the accompanying antigen. Lipid encapsulation is generally 25 accomplished using liposomes which are able to stably bind or entrap and retain nucleic acid. For a review of the use of liposomes as carriers for delivery of nucleic acids, see, Hug and Sleight, Biochim. Biophys. Acta. (1991) 1097:1-17; Straubinger et al., in Methods ofEnzymology (1983), Vol. 101, pp. 512-527. The KGF fragments of the present invention can be used for identification of 30 receptor recognition sites as well as for the design of peptide agonists or antagonists. 31 Moreover, in view of the unique specificity of KGF for keratinocytes, its inability to induce the proliferation of vascular endothelial cells or fibroblasts, and its lack of cytotoxicity, the truncated molecules and analogs thereof are a preferred agent of choice for wound healing applications, particularly where there is a desire to promote re 5 epithelialization of the skin. The truncated KGF molecules are also particularly useful in corneal epithelial repair. Other uses of the molecules include applications that utilize the specificity for epithelial cells found in the gastrointestinal tract. The fragments of the present invention may be conjugated to other molecules suitable for its intended use. For example, the KGF polypeptides can be conjugated to a 10 toxin molecule, such as ricin A, diphtheria toxin, or saporin for destruction of its target cell, i.e., epithelial cells, particularly, keratinocytes. Such KGF toxin conjugates suitable for use herein can be produced by methods known in the art, for example, U.S. Pat. Nos. 4,771,128, 4,808,705, and 4,894,443 and International Publication No. WO 92/04918. The compositions of the invention comprise the molecules described above, 15 together with one or more pharmaceutically acceptable excipients or vehicles, and optionally other therapeutic and/or prophylactic ingredients. Such excipients include liquids such as water, saline, glycerol, polyethyleneglycol, hyaluronic acid, ethanol, etc. Suitable excipients for nonliquid formulations are also known to those of skill in the art. Pharmaceutically acceptable salts can be used in the compositions of the present invention 20 and include, for example, mineral acid salts such as hydrochlorides, hydrobromides, phosphates, sulfates, and the like; and the salts of organic acids such as acetates, propionates, malonates, benzoates, and the like. A thorough discussion of pharmaceutically acceptable excipients and salts is available in Remington's Pharmaceutical Sciences, 18th Edition (Easton, Pennsylvania: Mack Publishing 25 Company, 1990). Additionally, auxiliary substances, such as wetting or emulsifying agents, biological buffering substances, surfactants, and the like, may be present in such vehicles. A biological buffer can be virtually any solution which is pharmacologically acceptable and which provides the formulation with the desired pH, i.e., a pH in the physiologically 32 acceptable range. Examples of buffer solutions include saline, phosphate buffered saline, Tris buffered saline, Hank's buffered saline, and the like. Particularly preferred compositions are those which are applied topically, such as ointments, pastes, powders, dressings, creams and plasters. Such topical compositions 5 may also include topical anesthetics, such as but not limited to, benzocaine, lidocaine, dibucaine, dyclonine hydrochloride, pramoxine hydrochloride, proparacaine hydrochloride, tetracaine, benoxinate hydrochloride, butamben picrate, clove oil and eugenol, as well as combinations and derivatives of the above. Ophthalmic compositions for direct delivery for the eye are also of particular use with the KGF fragments of the 10 invention. See, e.g., Remington's Pharmaceutical Sciences, 18th Edition (Easton, Pennsylvania: Mack Publishing Company, 1990) for a discussion of various topical and ophthalmic compositions. As explained above, once formulated, the compositions of the invention are generally administered topically or ophthalmically. However, other modes of 15 administration include parenteral administration, for example, intravenously, intra arterially, intra-articularly (e.g., into the knee), subcutaneously, intradermally, intramuscularly, transdermally, intranasally, mucosally, and by aerosol administration. For example, the composition can be administered by inhalation, e.g., as a nasal or mouth spray or aerosol. The compositions may also be delivered in situ, e.g., by implantation. 20 A pharmaceutically or therapeutically effective amount of the composition will be delivered to the subject. The precise effective amount will vary from subject to subject and will depend upon the species, age, the subject's size and health, the nature and extent of the condition being treated, recommendations of the treating physician, and the therapeutics or combination of therapeutics selected for administration. Thus, the 25 effective amount for a given situation can be determined by routine experimentation. For purposes of the present invention, generally a therapeutic amount if administered topically will be in the range of about 0.1 ig/cm 2 of wound to approximately 500 pg/cm 2 of wound, preferably about 1 jg/cm 2 of wound to approximately 100 pg/cm 2 of wound, more preferably about 1-10 pg/cm 2 of wound to approximately 50 pg/cm 2 , .or any integer 30 between these values, such as 21, 22, 23, 24...30, 31, 32, 33, 34...40, 41, 43...50...60..., and 33 so on. For parenteral administration, typical doses will be in the range of about 0.01 jig/kg body weight/day to about 100 ttg/kg/day, more preferably about 0.1 pig/kg/day to about 80 pg/kg/day, more preferably 1 jg/kg/day to about 40 jg/kg/day in one or more doses. Typically, if polynucleotides are delivered, the doses will be at least an order of magnitude 5 lower. The subject may be administered as many doses as is required to reduce and/or alleviate the signs, symptoms, or causes of the disorder in question, or bring about any other desired alteration of a biological system. In any event, the amount of KGF fragment present in the subject composition is an amount less than the amount of KGF,.

3 necessary in order to obtain an equivalent response. This amount is readily determined by 10 comparing the bioactivity of the fragment in question to that of KGF,, 3 , as described above. III. Experimental Below are examples of specific embodiments for carrying out the present 15 invention. The examples are offered for illustrative purposes only, and are not intended to limit the scope of the present invention in any way. Efforts have been made to ensure accuracy with respect to numbers used (e.g., amounts, temperatures, etc.), but some experimental error and deviation should, of course, be allowed for. 20 Enzymes were purchased from commercial sources, and used according to the manufacturers' directions. Radionucleotides and nitrocellulose filters were also purchased from commercial sources. In the cloning of DNA fragments, except where noted, all DNA manipulations were done according to standard procedures. See, Sambrook et al., supra. Restriction 25 enzymes, T 4 DNA ligase, DNA polymerase I, Klenow fragment, and other biological reagents were purchased from commercial suppliers and used according to the manufacturers' directions. Double-stranded DNA fragments were separated on agarose gels. 34 Example 1 Construction of truncated KGF Yeast Vectors for Intracellular Yeast Expression This example describes a procedure for construction of yeast expression vectors for 5 intracellular expression of the various truncated KGF molecules for use with the subject invention. The expression vectors included the particular truncated KGF coding sequence under the control of the ADH2/GAPDH promoter, a hybrid yeast promoter. In particular, for each different truncation, two oligos were used (see below), a top strand and a bottom 10 strand. The oligos were annealed and then placed in a ligation reaction. The ligation reactions included the plasmid pSI3, cut at Nco and Sal sites, a Kpn/Sal fragment, encoding a truncated KGF and one of the annealed oligo pairs. The oligo pairs encoded the amino terminus of the particular truncated KGF protein desired and were designed to link the Nco site of the vector to the Kpn site of the KGF encoding fragment. Plasmid 15 pSI3 is a derivative of pYASII, which was deposited with the ATCC, Manassas, VA, on Feb. 27, 1985, and assigned ATCC Accession No. 20745. The construction of pYASI1 is described in U.S. Pat. No. 4,751,180. The various oligos for the completed truncated KGFs were as follows. The "X" in the oligo sequences represents a 5' phosphate group. 20 KGFdesI- 5 5' XCATGAGCAGCCCTGAGCGACACACAAGAAGTrATGATTACATGGAAGGAG GGGATATAAGAGTGAGAAGACTCTTCTGTCGAACACAGTGGTAC 3' (SEQ ID NO:1) 25 5' XCACTGTGTTCGACAGAAGAGTCTTCTCACTCTTATATCCCCTCCTTCCATGT AATCATAACTTCTTGTGTGTCGCTCAGGGCTGCT 3' (SEQ ID NO:2) 35 K desl1-18: 5' XCATGGAGCGACACACAAGAAGTTATGATTACATGGAAGGAGGGGATATAA GAGTGAGAAGACTCTTCTGTCGAACACAGTGGTAC 3' (SEQ ID NO:3) 5 5' XCACTGTGTTCGACAGAAGAGTCTTCTCACTCTTATATCCCCTCCTTCCATGT AATCATAACTTCTTGTGTGTCGCTC 3' (SEQ ID NO:4) KGFd~sl- 1 9 : 5' XCATGCGACACACAAGAAGTTATGATTACATGGAAGGAGGGGATATAAGAG 10 TGAGAAGACTCTTCTGTCGAACACAGTGGTAC 3' (SEQ ID NO:5) 5' XCACTGTGTTCGACAGAAGAGTCTTCTCACTCTTATATCCCCTCCTTCCATGT AATCATAACTTCTTGTGTGTCG 3' (SEQ ID NO:6) 15 KGFA - 20 : 5' XCATGCACACAAGAAGTTATGATTACATGGAAGGAGGGGATATAAGAGTGA GAAGACTCTTCTGTCGAACACAGTGGTAC 3' (SEQ ID NO:7) 5' XCACTGTGTTCGACAGAAGAGTCTTCTCACTCTTATATCCCCTCCTTCCATGT 20 AATCATAACTTCTTGTGTG 3' (SEQ ID NO:8) K des1-21: 5' XCATGACCAGAAGTTATGATTACATGGAAGGAGGGGATATAAGAGTGAGAAGACTCTT CTGTCGAACACAGTGGTAC 3' (SEQ ID NO:9) 25 5' XCACTGTGTTCGACAGAAGAGTCTTCTCACTCTTATATCCCCTCCTTCCATGTAATCATA CTTCTGGT 3' (SEQ ID NO:10) KG s- 2 2 30 5' XCATGAGAAGTTATGATTACATGGAAGGAGGGGATATAAGAGTGAGAAGA 36 CTCTTCTGTCGAACACAGTGGTAG 3' (SEQ ID NO: 11) 5 XCACTGTGTTICGACAGAAGAGTCTrCTCACTCTTATATCCCCTCCTT~CCATGT AATCATAACTFPCT 3' (SEQ ID NO: 12) 5 KG~desl- 24 : 5' XCATGTATGAflACATGGAAGGAGGGGATATAAGAGTGAGAAGACTCTTCT GTGGAACACAGTGGTAC 3' (SEQ ID NO: 13). 10 5'XCACTGTGTTCGACAGAAGAGTCTTCTCACTCTTATATCCCCTCCTTCCATGT AATCATA 3' (SEQ ID NO: 14) KQd,25: 5' XCATGGATTACATGGAAGGAGGGGATATAAGAGTGAGAAGACTC'PTJTGTC 15 GAACACAGTGGTAC 3' (SEQ ID NO: 15) 5'CCGGTGCGAATTCCCCTTTCCCTCAG AATC 3' (SEQ ID NO: 16) 20 KGFCSZ 6 5'XCATGTACATGGAAGGAGGGGATATAAGAGTGAGAGACTC'CTGTCGA CAGAGTGGTAC 3' (SEQ ID NO: 17) 5'XCACTGTGTTCGACAGAAGAGTCTTCTCACTCflATATCCCGTCCTlCCAT 25 GTA 3' (SEQED NO: 18) 5'XCATGGGGGATATAAGAGTGAGAAGACTCTTrCTGTCGAACACAGTGGTAC 3' (SEQ ID NO: 19) 30 37 5' XCACTGTGTTCGACAGAAGAGTCTTCTCACTCTTATATCCCC 3' (SEQ ID NO:20) KGFdesl-35: 5 5' XCATGAGAAGACTCTTCTGTCGAACACAGTGGTAC 3' (SEQ ID NO:21) 5' XCACTGTGTTCGACAGAAGAGTCTTCT 3' (SEQ ID NO:22) KGFd 137 : 10 5' XCATGCTCTTCTGTCGAACACAGTGGTAC 3' (SEQ ID NO:23) 5' XCACTGTGTTCGACAGAAGAG 3' (SEQ ID NO:24) Additionally, expression vectors encoding mature, full-length KGF (KGF, 1 3 ) and a 15 truncated KGF molecule with a deletion of the first 23 N-terminal amino acids, KGFcsl- 23 , were produced. See, e.g., U.S. Patent No. 5,677,278. An analog of KGFcsi- 22 , with an alanine residue at the N-terminus instead of the naturally occurring arginine, was also produced. 20 Example 2 Intracellular Expression of Truncated KGF Molecules by Yeast Cells The expression vectors described above were used to transform Saccharoinyces cerevisae cells by lithium acetate transfection, using standard methods. See, e.g.,"Guide to Yeast Genetics & Molecular Biology," Methods in Enzymology, Vol. 194 (Academic 25 Press, 1991). Transformants were selected on uracil-deficient media having 2% glucose. Transformants were incubated overnight in 5 ml of leucine-deficient media with 5% glucose at 30*C in a shaking apparatus. The culture for production of the recombinant truncated molecules was a 20 ml culture, seeded with the overnight culture in YEP medium with 2% glucose for approximately 72 hours. 30 38 Example 3 Purification of the Truncated Recombinant KGF Molecules The cultures from above were centrifuged to form a yeast cell paste and cells were lysed in 10 mM MgCl 2 , 50 mM Tris, pH 8.0, 0.1 M dithiothrietol (DTT), using standard 5 techniques. The cell lysate was generated as a batch, using glass beads in a Dynomill DKL-Pilot. Lysate generation was complete when cell breakage was >95%. The homogenate was cooled to 4-8'C by using a suitable heat exchanger. Debris was then removed by centrifugating the lysate at 15,000 g for thirty minutes at 4"C. The NaCI concentration of the supernatant was adjusted to 0.5 M NaCl and recombinant, truncated 10 KGF was purified from the supernatant as follows. A. Heparin SepharoseTM Affinity Chromatography Supernatant obtained as described above was immediately applied to a Heparin Sepharose (HS) resin column. The lysed product was allowed to run for approximately 30 15 minutes at 4*C through a 30 ml bed of HS resin. The column was equilibrated in a buffer containing 0.5 M NaCl, 0.1 M DTT and 10 mM Tris-HCl at pH 7.3. Once the cell lysate was loaded, the column was washed extensively with the equilibration buffer until the absorbance at 280 mn returned to baseline. Protein was eluted from the HS column with an increasing step-wise NaCl gradient. The NaCl concentrations were 1 M and 2 M NaCl, 20 in 10 mM Tris-HCl at pH 7.3, 0.1 M DTT. The flow rate of the column during elution was approximately 90 ml/hr and 4 ml size fractions were collected. The fractions were tested for KGF bioactivity utilizing Balb/Mk cells. The assay is described below. The fractions with the highest bioactivity were eluted with I M NaCI and were pooled. Before the pooled fractions were loaded onto the next column, the 25 fractions were dialyzed overnight against 0.2 M NaCl, 10 mM Tris-HCl at pH 7.3. 39 B. Mono S Cation Exchange Chromatography The pooled fractions eluted from the HS column were loaded with a Super loop onto a Mono S column linked to a fast high pressure liquid chromatography (FPLC) system (Pharmacia, Piscataway, N.J.). The Mono S cation exchange column was 5 equilibrated with 10 mM Tris at pH 7.3. When the pooled fractions were loaded, the column was washed extensively at a flow rate of 1 ml/min. with the equilibration buffer until the absorbance returned to baseline. Then, protein was eluted from the column with a linear NaCI gradient, 0.2 M to 1 M NaCl in 10 mM Tris, at pH 7.3 at a flow rate of 1 mi/min., and 1 ml fractions were collected. 10 A major protein peak eluted at about 0.6 M NaCl. Fractions across the protein peak were assayed for bioactivity and active fractions were pooled and subjected to SDS-PAGE analysis. The protein concentration of the pooled fractions was determined by Bradford assay according to instructions accompanying the protein assay kit from BioRad (Richmond, Calif., USA). 15 Example 4 KGF Bioactivity Assay Utilizing Balb/Mk Cells KGF bioactivity was assessed by the ability of the pooled 0.6 M NaCl fractions to promote growth of Balb/Mk cells. In particular, stock cultures of Balb/Mk cells were 20 grown and maintained in low calcium Dulbecco's modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum, 0.25 pg/ml fungizone, and 10 ng/ml acidic FGF (aFGF). The cells were incubated at 37*C in a 10% CO 2 atmosphere with 99% humidity. For the bioactivity assay, the cells were seeded in 12-well plates at a density of 5 x10 3 cells per well in 1 ml of medium as described above for the stock cultures (except 25 that the seeding medium contained no aFGF), and as described in Gospodarowicz et al. J. Cell. Physiol. (1990) 142: 325-333. Ten microliter aliquots of the desired column fractions were diluted into I ml of 0.2% (w/v) gelatin in phosphate buffered saline (PBS). Ten microliters of this dilution were added to Balb/Mk cells seeded in 12-well cluster plates containing 22 mm wells, at 5 40 x10 3 cells per well, and a 10 sil aliquot of either the diluted column fractions or medium containing 10 ng aFGF were added to the cells every other day. After seven days in culture, the cells were trypsinized and the final cell density was determined using a CoulterTM counter (Coulter Electronics, Hialeah, Fla., USA). The cells 5 were released from the plates by replacing the culture medium with a solution containing 0.9% NaCl, 0.01 M sodium phosphate (pH 7.4), 0.25% trypsin, and 0.02% EDTA (STV). The cells were incubated in this solution for 5-10 minutes at 37*C and then the stock culture medium was added to the cells. The cells were then counted using a CoulterTM counter. The final cell density was graphed as a function of column fraction protein 10 concentration. The protein concentration was graphed on a log scale. The ED 5 0 was calculated by (a), dividing in half the difference between the lowest and highest cell density value of the curve; and (b), determining from the graph what protein concentration corresponds to that cell density number obtained in (a). Results of a typical bioassay for KGFes- 22 , KGFesI- 23 , KGFdecs- 2 4 , KGFcsI-26 and 15 KGFe,S.

3 O are shown in Figure 2A. A similar comparison was done with KGFes- 2 3 , KGFesi- 26 , KGFdes -,0, acidic FGF (aFGF) and full-length KGF (FL-KGF) and results are shown in Figure 2B. When the ED,, of the different truncated forms was normalized to that of native KGF taken as 100% (Table 1) KGFesi-22, KGFdsi- 23 and KGFcSI-24 had significant increases in their biological activity. In particular, KGFesi-22 was 20 approximately 6-fold more active, while KGFes- 23 and KGFeSZ- 24 were 10-fold more active than native KGFi 63 . Even when compared on a per molecule basis and adjusted for molecular weight, these species displayed greatly enhanced activity relative to KGF 63 . Deletion beyond KGFcsi- 24 to KGFdei- 26 rendered the truncated forms of KGF with comparable activity to the native form, and deletions beyond KGFesi- 2 & led to a reduction 25 in biological activity. It should be noted that KGFd,, 3 , which retained only 2-3% of the biological activity of the native form, was as active as aFGF when tested on Balb/Mk cells. Since this form is more homologous to aFGF than any of the other truncations, the question was raised as to whether this truncated form of KGF might have lost the target cell specificity 30 peculiar to KGF. As explained above, KGF only stimulates cells of epithelial origin in 41 contrast to other forms of FGF which have a wide range of target cells, and endothelial cells in particular. However, this apparently does not occur since when tested on either adrenal cortex-derived capillary endothelial cells (ACE) or adult bovine aortic endothelial cells (ABAE) cells (see below), in the presence or absence of heparin, KGFcs_ 3 5 , as well 5 as other shorter truncations, were inactive (Figure 3). Table 1. Mitogenic Activity of KOF Polypeptides KGF Polypeptide Average % MW of Average % Relative KGFm)** Relative Activity Activity (per unit (on a per weight molecule basis)* basis) Full Length (KGF 16 3 ) 100 18882 100 10 KGFcsI I 154 17229 141 KGFdes1 8 166 16958 149 KGFdes1 9 100 16829 89 KGFesI-20 187 16673 165 KGFdes1-21 200 16536 175 15 KGF. I- 2 2 600 16435 522 KGFdcsI- 23 1000 16278 862 KGFes1- 24 1000 16191 857 KGFdesi-2s 150 16028 127 KGFcsI-2 6 100 15913 84 20 KGFdesIl 3 O 28.5 15433 23 KGFee,.

3 s 3 14892 2 42 *ED50 of (KGFm - KGF 163 ) X 100 **Molecular weight (MW) was calculated using the Vector NTI program (December 22, 1999), Infomax, Inc. 5 The activity of an analog of KGFacs- 22 , with an alanine substituted for the naturally occurring N-terminal arginine, expressed in E. coli, was compared to an E. coli-expressed 10 KGFacs- 2 3 . The analog was tested multiple times and showed an ED 5 0 similar to that of E. coli-expressed

KGFI-

23 . Example 5 KGF Bioactivity on ABAE or ACE Cells 15 KGF can be characterized by its lack of activity on vascular endothelial cells derived from large vessels (adult bovine aortic endothelial cells, ABAE) or capillary cells (adrenal cortex-derived capillary endothelial cells, ACE) as compared with other forms of FGF, such as basic FGF (bFGF) or acidic FGF (aFGF). To analyze whether the various truncated forms of FGF retained this cell specificity, their biological activity on 20 endothelial cells was tested. Stock cultures of ABAE and ACE cells were grown and maintained in Dulbecco's modified Eagle medium supplemented with 10% bovine serum, 0.25 pig/ml fungizone, and 2 ng/ml bFGF. The cells were incubated at 37 0 C with a 10% CO 2 concentration and 99% humidity. 25 In the mitogenic assay, either 5 x 10' ABAE or ACE cells were plated per well in 12-well plates in stock culture medium, as described in Gospodarowicz et. al. Proc. Natl. Acad. USA (1976) 73:4120-4124; Gospodarowicz et. al. J. Cell. Physiol. (1976) 127:121-136; and Gospodarowicz et. al. Proc. Natl. Acad. USA (1989) 86:7311-7315. Saturating concentrations of KGF,,,, as well as the various truncated variants of 30 KGF and basic FGF were added every other day. After 7 days in culture, cells were 43 trypsinized as described for the Balb/MK cell cultures and the final cell density was determined using a Coulter counter. As shown in Figure 3, all of the tested N-terminally truncated KGF polypeptides lacked activity on both ABAE and ACE as compared with bFGF. The truncations were 5 based, in part, on structure alignment with acidic FGF. The results confirm that the potency of native KGF can be changed to that of acidic FGF without changing cell specificity. Acidic FGF is a known mitogen for Balb/Mfk cells and its potency is 10-fold less than that of KGF. This reflects its lower binding affinity for the KGF receptor. Removal of the first 30 to 35 amino acids of native KGF leads to the strong decrease in 10 biological activity shown by those truncated analogs (28.5 and 3% that of native KGF). At the same time, the degree of homology between acidic FGF and KGF increases when cell structural determinants for interacting with the FGF receptor are kept. Thus, KGF analogs with longer deletions behave more like acidic FGF. Surprisingly, even with the longest deletion, the target cell specificity typical of KGF is kept and the analogs, in 15 contrast with acidic FGF or basic FGF, are not mitogenic for vascular endothelial cells. Example 6 Thermal Stability Studies The ability of native KGF and various N-terminally truncated KGF polypeptides to 20 withstand elevated temperatures was examined. Samples containing 0.1 mg/ml protein were prepared in Ca" Mg"-free PBS and 100 Rl of each sample was aliquoted into 1 ml plastic vials. The vials were sealed and placed in a 37*C incubator. At predetermined time intervals, vials were withdrawn and analyzed for the loss of soluble proteins. 30 pl aliquots were analyzed by SDS-PAGE electrophoresis. SDS-PAGE was 25 performed on an electrophoresis system (Novex, San Diego, California, U.S.A.) using Tris-glycine precast gels (5% to 20% acrylamide, according to the method of Laemmli Nature (1970) 227:680-685). Samples were mixed with reducing or non-reducing SDS sample buffer without heating before loading. The proteins were detected by Coomassie blue staining. The stained gels were 30 scanned by densitometry using a BioRad Model GS 700 Imaging densitometer 44 (Richmond, Calif., USA). The amount of soluble protein was determined by integrating the stained-band area and plotting the result as a function of time of incubation at 37'C. The half-life for the loss of soluble monomeric protein was then estimated from these kinetic curves. 5 The biological activity of the samples at various time intervals was also determined using the Balb/Mk cell proliferation assay as described above. The half-life for biological activity of the various samples was determined by plotting the ED,, of the samples as a function of time of incubation at 37*C. In particular, the thermal stability and oligomer formation of native KGF (KGF 63 ) 10 verses KGFei- 23 was assessed by incubating the KGF polypeptides (184 ig/ml native KGF and 138 pig/ml KGFdes.- 23 , diluted in PBS) for periods ranging from 0 to 8 days at 37*C. Aliquots were taken daily and their potency assessed on Balb/Mk cells, while oligomer formation was assessed by SDS-PAGE. When analyzed by SDS-PAGE, native KGF formed dimers readily. Dimerization was far less evident for KGFdeSI.

23 . Also 15 striking was the decrease in staining as a function of time for the monomeric form of native KGF. Again, this was far less evident for KGFdesI- 23 The cell proliferation assay indicated that under these conditions, the biological half-life of native KGF was 2 days while that of KGFSi- 2 3 was 7 days. Thermal stability studies also indicated that KGFe,I.IS, KGFst-18, KGFS.

1 9 , 20 KGFdes1-20, KGFdcsI-21, KGFdesl-22, KGFesI-24, KGFesI.2s, KGFesI- 26 , KGFdesI30 and KGFee, 1 .35 were more stable than the full-length KGF, although by varying degrees. A typical stability study which lasted 24 days with samples taken every 3 days is shown in Figure 4. In this study, KGFdeSI- 2 4 and KGFe,1.15 (100 pg/ml) were diluted in PBS and incubated at 37*C. Samples were analyzed under non-reducing conditions by SDS-PAGE. Gels were 25 then stained and scanned by densitometry. Extension of the thermal stability studies from 9 days to 24 days permitted a more accurately defined half-life solution of KGFdcsI-23 versus native KGF. As shown in Figure 5, the half-life of native KGF was 7 days versus17 days for KGFSI.

23 . 45 These results, taken together, indicate that the difference in biological activity between native KGF and the truncated molecules might be caused, in part, by differing thermal stability. 5 Example 7 Acid Stability at pH 2.1 To follow the formation of oligomer as a function of time, native KGF and KGFc.i- 2 3 samples, maintained for various time intervals at 37*C, were analyzed by reverse phase HPLC. 100 pl samples were diluted to 1 ml in 0.1% trifluoroacetic acid 10 (TFA) in water, pH 2.1. The sample was then applied to a Vydac C 4 column (0.46 cm x 25 cm, 5 gm particle size, 300 A* pore size) equilibrated in 0.1% (v/v) TFA. Protein was eluted with a linear 115 min multilinear acetonitrile gradient in (20-100%). The absorbance peaks were analyzed for their protein content by integrating their surface area. The amount of monomeric protein was plotted as a function of time. The half-life for the 15 monomeric protein was then estimated as described above. To determine the formation of oligomers, samples were also analyzed under non-reducing conditions by SDS-PAGE. Biological activity of the various protein peaks was also determined using the Balb/Mk cell proliferation assay as described above. In particular, native KGF (28 gg/ml) and KGFdesi- 2 3 (36 gg/ml) were diluted in PBS 20 and incubated for periods of time ranging from 0 to 9 days at 37'C and aliquots were analyzed daily by reverse phase high pressure liquid chromatography (RP-HPLC) under acid conditions (pH 2.1). A single peak of UV absorbance was observed which eluted at 60 minutes for both native and KGFdesi- 2 3 . The intensity of the peak decreased as a function of time. However, the decrease was far more pronounced for native KGF than 25 KGFcsZ- 23 , so that by day 9, integration of both peaks indicated that the amount of native KGF was one-third that of KGFeSI- 2 3 . The decrease in mass of native KGF was associated with a drastic decrease in biological activity. By day 7, native KGF had become inactive. In contrast, KGFdcs1-23 still retained 50% of its original biological activity (Figure 6). 46 Example 8 Comparison of the Bioactivity of Native KGF versus KGFd, 1 2 3 when Added Only Once Versus Every Other Day In the cell proliferation assay described above, increasing concentrations of native 5 KGF and KGFde,1-23 were added every other day. If stability is the mechanism for enhanced activity, there should still be the same difference in dose response when native KGF or truncated KGF polypeptides are added once, rather than every other day, even though overall cell proliferation is less. To test this hypothesis, varying concentrations of KGFC.IS, KGF,1- 23 and KGFcsI.- 2 5 were added only once, in the concentrations shown in 10 Figure 7. As shown in Figure 7 and Table 2, the same difference in biological activity was observed with KGFdasi- 2 3 . In particular, KGFe,1- 23 was 13-fold more active than native KGF when added once, versus 10-fold more active when added every other day. Additionally, both the KGFde.,,I, and KGFsi-2s polypeptides showed an increase in activity over native KGF. This confirms that increased stability is one of the mechanisms 15 which contributes to increased potency. Table 2. Comparison of the Biological Activity of Native Versus Truncated KGFs Added Either Once or Every Other Day 20 Average % Activity KGF Polypeptide Added Once Added Every Other Day Full Length 100 100 KGFdesI-I 8 173 150 KGFdesl- 23 1320 1000 25 KGFcs,-2 5 173 160 47 Example 9 Is Protease Contamination Responsible for the Rapid Disappearance of Native KGF To rule out the possibility that protease contamination of the native KGF preparation, absent from the KGFCSi-l 23 preparation, was responsible for the rapid 5 disappearance of native KGF, the following experiment was done. Native KGF and KGFcs- 23 (100 gg/ml) were diluted in PBS and incubated at 37"C either alone or mixed at an equal ratio (v/v). Samples were taken on day 3, 6, 9 and 12 and analyzed by SDS PAGE, as described above. After staining, the gels were scanned by densitometry. If the native KGF disappeared and KGFd,-, 2 3 did not, protease contamination would be ruled 10 out. Native KGF disappeared by day 6. However, KGFSI.

23 could still be seen after 12 days, eliminating the possibility of protease contamination as a reason for the disappearance of native KGF. Surprising however, was the difference in the band-staining intensity when KGFdecI- 23 was incubated alone versus incubated in combination with native 15 KGF. The staining intensity of the KGFacs- 23 band was far greater when incubated alone than when mixed with the native KGF. It is possible that when KGFacs- 23 is incubated with native KGF, complexes form and KGFCSI- 23 is taken out of solution. When the biological activity of native KGF versus KGFesi- 23 was analyzed, a 10 fold increase in potency was observed which correlated with greater thermal stability of 20 the truncated form versus the native form. Thus, increased stability could in part explain the increased potency. This however does not appear to be the only mechanism contributing to increased activity. Without being bound by a particular theory, the lack of activity of native KGF may be due to precipitation out of solution. When various truncations above were analyzed for stability and biological activity, they showed 25 increased stability and biological activity as compared to the native form. However, there was little correlation between increased stability and increased biological activity. For example, KGFdesI-IS and KGFcsI- 24 had comparable thermal stability but KGFesI- 24 was 10 fold more potent than native KGF while KGFdeI.lS was 1.5-fold more active. Therefore other mechanisms likely exist which contribute to the increased potency of the most potent 30 molecules, KGFcsi-22, KGFcsi- 23 , and KGF,,1- 2 4 . It is also remarkable that the domain 48 conferring maximum increase in potency is limited, consisting of three residues. These three residues may therefore provide optimum conformation of KGF in its interaction with the receptor. Truncation confirms that the potency of native KGF can be changed to that of 5 aFGF without changing cell specificity. Acidic FGF is a known mitogen for Balb/Mk cells and it is 10-fold less active than KGF. This is due to its lower binding affinity for the KGF receptor. Removal of the first 30 to 35 amino acids led to a strong decrease in biological activity of the truncated polypeptides (28.5% and 3%, respectively, of native KGF). At the same time, the longer deletions increase the degree of homology between 10 aFGF and KGF and increase cell structural determinants for interacting with the FGF receptor. Thus, the longer the deletions, the more like aFGF the truncated KGF polypeptides behave. Surprisingly, even the longest deletion retained the target cell specificity typical of KGF, i.e., for cells of epithelial origin, and the shortest polypeptides, in contrast with aFGF or bFGF, were not mitogenic for vascular endothelial cells. These 15 findings, that the NH 2 terminal domain of KGF does not seem to be involved in its cell specificity, at least as far as the first 35 residues are concerned, are in contrast with earlier reports (see, e.g., International Publication No. WO 90/08771). Example 10 20 In Vivo Efficacy of N-Terminally Truncated KGF Polypeptides Full-length KGF (KGF 6 3 ) and an N-terminally truncated molecule, KGFest- 2 3 , were tested in a rat model of healing of a surgical colonic anastamosis. In particular, KGF formulations, containing 5 mg/ml KGF 6 ,6 or 1 mg/ml KGFeesi.

23 were administered intraperitoneally to rats. Colonic crypt depth (in p1m), a measure of cellular proliferation 25 related to healing, and busting pressure in mm mercury, a measure of strength of the healed wound, were measured on days 2, 4 and 7 for rats given KGF,,, and on days 2, 4 and 6 for rats given the truncated molecule. Results are shown in Tables 3 and 4. In particular, 1 mg/ml of the truncated molecule was as effective or more effective than 5 mg/ml of full-length KGF in promoting wound healing. Smaller doses of the 30 truncated molecule were also effective. Based on these results, it is likely that other N 49 terminally truncated KGFs, particularly KGFeSI-2 2 and KGFes- 24 , are also as effective, if not more effective, for wound healing using smaller doses than that required using KGF, 3 . Table 3. Colonic Crypt Depth in tm, Expressed as Percent of Vehicle Control 5 Day Measured Full Length KGF, 5 mg/kg Truncated KGF, 1 mg/kg 2 127 176 4 125 129 7 (6 of KGFdeSI- 2 3 ) 154 138 10 Table 4. Bursting Pressure in mm Hg, Expressed as Percent of Vehicle Control Day Measured Full Length KGF, 5 mg/kg Truncated KGF, 1 mg/kg 2 134 163 4 149 151 7 (6 of KGFesj- 23 ) 119 148 15 Thus, novel KGF compositions and methods for using the same are disclosed. Although preferred embodiments of the subject invention have been described in some detail, it is understood that obvious variations can be made without departing from the 20 spirit and the scope of the invention as defined by the appended claims. 50

Claims (13)

1. A method of treating a gastrointestinal disorder in a subject comprising administering to the subject a composition comprising a KGF polypeptide, wherein 5 said KGF polypeptide is selected from the group consisting of: (i) KGFdesiIS consisting of the contiguous amino acid sequence depicted at amino acid residues 19-163, inclusive, of Figure 1; (ii) KGFdesiI 9 consisting of the contiguous amino acid sequence depicted at amino acid residues 20-163, inclusive, of Figure 1; 10 (iii) KGFdesI-20 consisting of the contiguous amino acid sequence depicted at amino acid residues 21-163, inclusive, of Figure 1; (iv) KGFdesI-21 consisting of the contiguous amino acid sequence depicted at amino acid residues 22-163, inclusive, of Figure 1; (v) KGFdesI-22 consisting of the contiguous amino acid sequence depicted at 15 amino acid residues 23-163, inclusive, of Figure 1; (vi) KGFdesI-24 consisting of the contiguous amino acid sequence depicted at amino acid residues 25-163, inclusive, of Figure 1; (vii) KGFdesI-25 consisting of the contiguous amino acid sequence depicted at amino acid residues 26-163, inclusive, of Figure 1; 20 (viii) a biologically active analog of (i), (ii), (iii), (iv), (v), (vi), or (vii), wherein said biologically active analog consists of the same number of amino acids as (i), (ii), (iii), (iv), (v), (vi), or (vii), respectively, and has at least 70% sequence homology thereto; and (ix) a biologically active analog of (i), (ii), (iii), (iv), (v), (vi), (vii) or (viii), 25 wherein said analog consists of the amino acid sequence of (i), (ii), (iii), (iv), (v), (vi), (vii) or (viii), respectively, and an additional N-terminal methionine, wherein said KGF polypeptide exhibits an increase in bioactivity relative to mature, full-length, KGF (KGF 1 63 ) as determined by the Balb/MK bioactivity assay and further wherein said KGF polypeptide is present in a therapeutically effective amount 30 and the therapeutically effective amount is 75% or less of the amount on a per molecule basis of KGF 1 63 needed to elicit an equivalent therapeutic response.

2. The method of claim 1, wherein the KGF polypeptide is KGFdesi- 22 consisting of the contiguous amino acid sequence depicted at amino acid residues 23-163, inclusive, 35 of Figure 1, or a biologically active analog thereof wherein said biologically active analog consists of 141 amino acids and has at least 70% sequence homology thereto, and wherein the therapeutically effective amount is 50% or less of the amount on a per molecule basis of KGF 16 3 needed to elicit an equivalent therapeutic response.

3. The method of claim 2, wherein the KGF polypeptide is KGFdesl- 22 consisting of 5 the contiguous amino acid sequence depicted at amino acid residues 23-163, inclusive, of Figure 1.

4. The method of claim 2, wherein the KGF polypeptide consists of the contiguous amino acid sequence depicted at amino acid residues 23-163, inclusive, of Figure 1 10 with the N-terminal arginine residue substituted with an alanine residue.

5. The method of claim 1, wherein the KGF polypeptide is KGFdesI- 24 consisting of the contiguous amino acid sequence depicted at amino acid residues 25-163, inclusive, of Figure 1, or a biologically active analog thereof wherein said biologically active 15 analog consists of 139 amino acids and has at least 70% sequence homology thereto, and wherein the therapeutically effective amount is 50% or less of the amount on a per molecule basis of KGF 163 needed to elicit an equivalent therapeutic response.

6. The method of claim 5, wherein the KGF polypeptide is KGFdesI- 24 consisting of 20 the contiguous amino acid sequence depicted at amino acid residues 25-163, inclusive, of Figure 1.

7. The method of claim 6, wherein the KGF polypeptide consists of the contiguous amino acid sequence depicted at amino acid residues 25-163, inclusive, of Figure 1 25 with the N-terminal arginine residue substituted with an alanine residue.

8. The method of any one of claims I to 7, wherein said biologically active analog consists of the same number of amino acids as (i), (ii), (iii), (iv), (v), (vi) or (vii), respectively, and has at least 80% sequence homology thereto. 30

9. The method of any one of claims 1 to 7, wherein said biologically active analog consists of the same number of amino acids as (i), (ii), (iii), (iv), (v), (vi) or (vii), respectively, and has at least 90% sequence homology thereto.

10. The method of any one of claims 1 to 9, wherein the therapeutically effective amount is 10% to 50% of the amount on a per molecule basis of the amount of full length KGF needed to elicit an equivalent therapeutic response. 5

11. The method of claim 10, wherein the therapeutically effective amount is 10% to 25% of the amount on a per molecule basis of the amount of full-length KGF needed to elicit an equivalent therapeutic response.

12. The method of claim 10, wherein the therapeutically effective amount is 10% to 10 20% of the amount on a per molecule basis of the amount of full-length KGF needed to elicit an equivalent therapeutic response

13. Use of a KGF polypeptide in the manufacture of a medicament for treating a gastrointestinal disorder, wherein said KGF polypeptide is selected from the group 15 consisting of: (i) KGFdesi-is consisting of the contiguous amino acid sequence depicted at amino acid residues 19-163, inclusive, of Figure 1; (ii) KGFdesI.19 consisting of the contiguous amino acid sequence depicted at amino acid residues 20-163, inclusive, of Figure 1; 20 (iii) KGFdesI-20 consisting of the contiguous amino acid sequence depicted at amino acid residues 21-163, inclusive, of Figure 1; (iv) KGFdes.-21 consisting of the contiguous amino acid sequence depicted at amino acid residues 22-163, inclusive, of Figure 1; (v) KGFdesI-22 consisting of the contiguous amino acid sequence depicted at 25 amino acid residues 23-163, inclusive, of Figure 1; (vi) KGFdesi- 2 4 consisting of the contiguous amino acid sequence depicted at amino acid residues 25-163, inclusive, of Figure 1; (vii) KGFdesI-25 consisting of the contiguous amino acid sequence depicted at amino acid residues 26-163, inclusive, of Figure 1; 30 (viii) a biologically active analog of (i), (ii), (iii), (iv), (v), (vi), or (vii), wherein said biologically active analog consists of the same number of amino acids as (i), (ii), (iii), (iv), (v), (vi), or (vii), respectively, and has at least 70% sequence homology thereto, wherein said KGF polypeptide exhibits an increase in bioactivity relative to 35 mature, full-length, KGF (KGF 163 ) as determined by the Balb/MK bioactivity assay and further wherein said KGF polypeptide is present in a therapeutically effective amount and the therapeutically effective amount is 75% or less of the amount on a per molecule basis of KGF 163 needed to elicit an equivalent therapeutic response.