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AU2002221824A1 - Methods for large scale protein production in prokaryotes - Google Patents

Methods for large scale protein production in prokaryotes

Info

Publication number
AU2002221824A1
AU2002221824A1 AU2002221824A AU2182402A AU2002221824A1 AU 2002221824 A1 AU2002221824 A1 AU 2002221824A1 AU 2002221824 A AU2002221824 A AU 2002221824A AU 2182402 A AU2182402 A AU 2182402A AU 2002221824 A1 AU2002221824 A1 AU 2002221824A1
Authority
AU
Australia
Prior art keywords
prokaryotes
methods
large scale
protein production
scale protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2002221824A
Inventor
Friedrich Goetz
Aranya Manosroi
Jiradej Manosroi
Chatchai Tayapiwatana
Rolf-Gunther Werner
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Boehringer Ingelheim International GmbH
Original Assignee
Boehringer Ingelheim International GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Ingelheim International GmbH filed Critical Boehringer Ingelheim International GmbH
Publication of AU2002221824A1 publication Critical patent/AU2002221824A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AU2002221824A 2000-11-14 2001-11-08 Methods for large scale protein production in prokaryotes Abandoned AU2002221824A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB0027782.2A GB0027782D0 (en) 2000-11-14 2000-11-14 Methods fro large scale protein productio in prokaryotes
GB0027782.2 2000-11-14
PCT/EP2001/012920 WO2002040696A2 (en) 2000-11-14 2001-11-08 Methods for large scale protein production in prokaryotes

Publications (1)

Publication Number Publication Date
AU2002221824A1 true AU2002221824A1 (en) 2002-05-27

Family

ID=9903155

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2002221824A Abandoned AU2002221824A1 (en) 2000-11-14 2001-11-08 Methods for large scale protein production in prokaryotes

Country Status (18)

Country Link
EP (1) EP1343909B1 (en)
JP (1) JP2004513648A (en)
KR (1) KR100857402B1 (en)
CN (1) CN100457909C (en)
AR (1) AR031336A1 (en)
AT (1) ATE440145T1 (en)
AU (1) AU2002221824A1 (en)
CA (1) CA2428643A1 (en)
CY (1) CY1110546T1 (en)
DE (1) DE60139635D1 (en)
DK (1) DK1343909T3 (en)
ES (1) ES2332124T3 (en)
GB (1) GB0027782D0 (en)
HK (1) HK1077848A1 (en)
MX (1) MXPA03004162A (en)
PT (1) PT1343909E (en)
UY (1) UY27021A1 (en)
WO (1) WO2002040696A2 (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6955910B2 (en) 2000-11-14 2005-10-18 Boehringer Ingelheim International Gmbh Method for large scale production of recombinant DNA-derived TPA or K2S molecules
GB0027779D0 (en) * 2000-11-14 2000-12-27 Boehringer Ingelheim Int Methods for large scale production of recombinant dna-derived tpa or k2s molecules
US7087412B2 (en) 2000-11-14 2006-08-08 Boehringer Ingelheim International Gmbh Methods for large scale protein production in prokaryotes
US9453251B2 (en) 2002-10-08 2016-09-27 Pfenex Inc. Expression of mammalian proteins in Pseudomonas fluorescens
US7985564B2 (en) 2003-11-21 2011-07-26 Pfenex, Inc. Expression systems with sec-system secretion
EP1774017B1 (en) 2004-07-26 2013-05-15 Pfenex Inc. Process for improved protein expression by strain engineering
BRPI0807834A2 (en) 2007-01-31 2014-07-22 Dow Global Technologies Inc "BACTERIAL LEADER SEQUENCES FOR INCREASED EXPRESSION".
US9580719B2 (en) 2007-04-27 2017-02-28 Pfenex, Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
US9394571B2 (en) 2007-04-27 2016-07-19 Pfenex Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
AU2010322205B2 (en) * 2009-11-17 2015-01-22 Janssen Biotech, Inc. Improved bacterial membrane protein secretion
CN102924602B (en) * 2011-08-08 2015-06-17 深圳市艾派格生物技术有限公司 Gold ion adsorption protein and method for enriching and recovering gold ion
CN106188311B (en) * 2016-07-18 2020-08-04 山东大学齐鲁医院 Preparation method and application of recombinant tumor necrosis factor-related apoptosis-inducing ligand protein

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5223407A (en) * 1988-08-31 1993-06-29 Allelix Inc. Excretion of heterologous proteins from e. coli
JPH05219963A (en) * 1990-07-18 1993-08-31 Applied Immune Sci Inc Temperature-inducing secretory expression vector and its use in procaryote cell
WO1997038123A1 (en) * 1996-04-05 1997-10-16 Board Of Regents, The University Of Texas System Methods for producing soluble, biologically-active disulfide bond-containing eukaryotic proteins in bacterial cells
EP1048732A1 (en) * 1999-04-26 2000-11-02 F. Hoffmann-La Roche Ag Process for producing natural folded and secreted proteins

Also Published As

Publication number Publication date
GB0027782D0 (en) 2000-12-27
DE60139635D1 (en) 2009-10-01
EP1343909B1 (en) 2009-08-19
WO2002040696A3 (en) 2002-09-19
DK1343909T3 (en) 2009-12-21
MXPA03004162A (en) 2004-04-20
UY27021A1 (en) 2002-07-31
KR20030051821A (en) 2003-06-25
HK1077848A1 (en) 2006-02-24
WO2002040696A2 (en) 2002-05-23
ATE440145T1 (en) 2009-09-15
EP1343909A2 (en) 2003-09-17
CN1628176A (en) 2005-06-15
ES2332124T3 (en) 2010-01-27
CY1110546T1 (en) 2015-04-29
PT1343909E (en) 2009-09-11
JP2004513648A (en) 2004-05-13
CN100457909C (en) 2009-02-04
AR031336A1 (en) 2003-09-17
CA2428643A1 (en) 2002-05-23
KR100857402B1 (en) 2008-09-08

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