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ATE500331T1 - Verfahren zur gleichzeitigen herstellung von multiplen proteinen; vektoren und zellen, die hierfür verwendung finden - Google Patents

Verfahren zur gleichzeitigen herstellung von multiplen proteinen; vektoren und zellen, die hierfür verwendung finden

Info

Publication number
ATE500331T1
ATE500331T1 AT03733632T AT03733632T ATE500331T1 AT E500331 T1 ATE500331 T1 AT E500331T1 AT 03733632 T AT03733632 T AT 03733632T AT 03733632 T AT03733632 T AT 03733632T AT E500331 T1 ATE500331 T1 AT E500331T1
Authority
AT
Austria
Prior art keywords
proteins
vectors
cells used
methods
simultaneous production
Prior art date
Application number
AT03733632T
Other languages
English (en)
Inventor
Arie Otte
Arthur Kruckeberg
Richard Sewalt
Original Assignee
Chromagenics Bv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chromagenics Bv filed Critical Chromagenics Bv
Application granted granted Critical
Publication of ATE500331T1 publication Critical patent/ATE500331T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/822—Reducing position variability, e.g. by the use of scaffold attachment region/matrix attachment region (SAR/MAR); Use of SAR/MAR to regulate gene expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT03733632T 2002-06-14 2003-06-13 Verfahren zur gleichzeitigen herstellung von multiplen proteinen; vektoren und zellen, die hierfür verwendung finden ATE500331T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP02077350 2002-06-14
PCT/NL2003/000432 WO2003106684A2 (en) 2002-06-14 2003-06-13 A method for simultaneous production of multiple proteins; vectors and cells for use therein

Publications (1)

Publication Number Publication Date
ATE500331T1 true ATE500331T1 (de) 2011-03-15

Family

ID=29724494

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03733632T ATE500331T1 (de) 2002-06-14 2003-06-13 Verfahren zur gleichzeitigen herstellung von multiplen proteinen; vektoren und zellen, die hierfür verwendung finden

Country Status (11)

Country Link
US (3) US7364878B2 (de)
EP (1) EP1513937B1 (de)
JP (3) JP4647309B2 (de)
AT (1) ATE500331T1 (de)
AU (1) AU2003238719B2 (de)
CA (3) CA2489475C (de)
DE (1) DE60336228D1 (de)
DK (1) DK1513937T3 (de)
ES (1) ES2362273T3 (de)
NZ (1) NZ537073A (de)
WO (1) WO2003106684A2 (de)

Families Citing this family (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5654442A (en) * 1989-11-14 1997-08-05 The Perkin-Elmer Corporation 4,7-dichlorofluorescein dyes as molecular probes
JP4021197B2 (ja) * 2000-03-17 2007-12-12 アンチキャンサー， インコーポレイテッド 遺伝子発現の全身光学イメージングとその使用
DE60236334D1 (de) * 2001-07-04 2010-06-17 Chromagenics Bv DNS-Sequenzen mit anti-repressor Aktivität
CA2489475C (en) 2002-06-14 2011-03-29 Chromagenics B.V. A method for simultaneous production of multiple proteins; vectors and cells for use therein
EP1513936A2 (de) * 2002-06-14 2005-03-16 Chromagenics B.V. Verwendung von repression hemmende sequenzen in verfahren zur genexpression verbesserung
CN101537180B (zh) 2002-07-18 2016-02-10 莫鲁斯有限公司 抗体混合物的重组生产
USRE47770E1 (en) 2002-07-18 2019-12-17 Merus N.V. Recombinant production of mixtures of antibodies
DK1573058T3 (da) * 2002-12-18 2012-01-16 Chromagenics Bv Fremgangsmåde til forbedring af proteinproduktion
EP1572994B1 (de) 2002-12-20 2007-02-21 Chromagenics B.V. Mittel und methoden zur produktion eines proteins durch chromatin-öffner, die chromatin zugänglicher für transkriptionsfaktoren machen können
WO2004106375A1 (en) 2003-05-30 2004-12-09 Merus Biopharmaceuticals B.V. I.O. Fab library for the preparation of anti vegf and anti rabies virus fabs
US20100069614A1 (en) 2008-06-27 2010-03-18 Merus B.V. Antibody producing non-human mammals
WO2005068622A2 (en) * 2004-01-20 2005-07-28 Merus B.V. Mixtures of binding proteins
CN1981057B (zh) 2004-07-08 2012-07-04 科罗迈吉尼科斯公司 改良核酸表达的新序列
US8999667B2 (en) 2004-11-08 2015-04-07 Chromagenics B.V. Selection of host cells expressing protein at high levels
US20060195935A1 (en) * 2004-11-08 2006-08-31 Chromagenics B.V. Selection of host cells expressing protein at high levels
DK1809750T3 (da) * 2004-11-08 2012-06-25 Chromagenics Bv Selektion af værtsceller, der udtrykker protein ved høje niveauer
US20100136616A1 (en) * 2004-11-08 2010-06-03 Chromagenics B.V. Selection of Host Cells Expressing Protein at High Levels
EP1809750B1 (de) * 2004-11-08 2012-03-21 ChromaGenics B.V. Selektion von wirtszellen mit proteinexpression auf hohem niveau
US8039230B2 (en) 2004-11-08 2011-10-18 Chromagenics B.V. Selection of host cells expressing protein at high levels
US20070042397A1 (en) * 2005-03-03 2007-02-22 International Business Machines Corporation Techniques for linking non-coding and gene-coding deoxyribonucleic acid sequences and applications thereof
KR101328300B1 (ko) * 2006-02-21 2013-11-14 크로마제닉스 비.브이. 고수준으로 단백질을 발현하는 숙주세포의 선택
US20080293086A1 (en) * 2006-09-18 2008-11-27 Cobalt Technologies, Inc. A Delaware Corporation Real time monitoring of microbial enzymatic pathways
KR20090075800A (ko) * 2006-09-20 2009-07-09 재단법인 한국파스퇴르연구소 세포에서의 표적 단백질 후보의 발현을 검출 및/또는 정량하는 방법 및 소형 분자 모듈레이터의 표적 단백질을 동정하는 방법
US20090081715A1 (en) * 2007-09-07 2009-03-26 Cobalt Technologies, Inc., A Delaware Corporation Engineered Light-Emitting Reporter Genes
BRPI0906892A2 (pt) 2008-04-09 2015-07-21 Cobalt Technologies Inc "método para a obtenção de um produto em um bioreator, sistema para a obtenção de um produto que compreende um bioreator, bioreator, bioreator para a fermentação de um produto biológico no suporte sólido e método para a obtenção de um produto biológico"
WO2010147462A2 (en) 2009-06-15 2010-12-23 Cellagenics B.V. Novel stringent selectable markers
CA2804912A1 (en) 2009-06-26 2012-12-29 Cobalt Technologies, Inc. Integrated system and process for bioproduct production
EP2582816B1 (de) 2010-06-15 2017-12-20 Cellagenics B.V. Neue intergenische elemente zur verstärkung der genexpression
DK2611915T3 (da) 2010-09-01 2015-06-22 Cellagenics B V Nukleinsyrefragmenter fra en ribosomal proteinpromoter til forstærkning af genekspression
US9617329B2 (en) 2010-10-01 2017-04-11 Csl Limited Method of cloning nucleic acid
WO2013078433A1 (en) 2011-11-23 2013-05-30 University Of Hawaii Auto-processing domains for polypeptide expression
SG10201913376XA (en) 2012-04-20 2020-02-27 Merus Nv Methods and means for the production of ig-like molecules
JP6343011B2 (ja) * 2013-10-07 2018-06-13 プレステージ バイオファーマ プライベート リミテッド 抗体発現用バイシストロニック発現ベクター及びそれを用いた抗体の生産方法
WO2024033441A1 (en) * 2022-08-09 2024-02-15 Geg Tech Transient expression system for rna
WO2024033444A1 (en) * 2022-08-09 2024-02-15 Geg Tech Transient expression system for rna, for cosmetic uses
WO2024033446A1 (en) * 2022-08-09 2024-02-15 Geg Tech Transient expression system for rna, for gene editing
WO2024033448A1 (en) * 2022-08-09 2024-02-15 Geg Tech Transient expression system for rna, for vaccination

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4228458A1 (de) * 1992-08-27 1994-06-01 Beiersdorf Ag Multicistronische Expressionseinheiten und deren Verwendung
US5610053A (en) 1993-04-07 1997-03-11 The United States Of America As Represented By The Department Of Health And Human Services DNA sequence which acts as a chromatin insulator element to protect expressed genes from cis-acting regulatory sequences in mammalian cells
US5972605A (en) 1994-07-07 1999-10-26 Geron Corporation Assays for regulators of mammalian telomerase expression
US5773695A (en) * 1996-01-26 1998-06-30 North Carolina State University Plant nuclear scaffold attachment region and method for increasing gene expression in transgenic cells
US5888809A (en) 1997-05-01 1999-03-30 Icos Corporation Hamster EF-1α transcriptional regulatory DNA
US6149212A (en) * 1997-07-02 2000-11-21 Eagle Inventors, Llc Adjustable door stop
AU771111B2 (en) 1998-07-21 2004-03-11 Emd Millipore Corporation A polynucleotide comprising a ubiquitous chromatin opening element (UCOE)
ES2292251T3 (es) * 1998-08-14 2008-03-01 Chromagenics B.V. Procedimiento de deteccion de una secuencia de adn, procedimiento de preparacion de una construccion de adn y uso de los mismos.
US6521419B1 (en) 1998-09-22 2003-02-18 Kanakaraju Koduri Expression vectors containing hot spot for increased recombinant protein expression in transfected cells
US6800457B2 (en) * 1998-09-22 2004-10-05 Bristol-Myers Squibb Company Expression vectors containing hot spot for increased recombinant protein expression in transfected cells
AU1128400A (en) * 1998-10-22 2000-05-08 Medical College Of Georgia Institute, Inc. Long terminal repeat, enhancer, and insulator sequences for use in recombinant vectors
US6586205B1 (en) * 2000-02-11 2003-07-01 Millennium Pharmaceuticals, Inc. 43239 a novel GPCR-like molecule and uses thereof
US20030166042A1 (en) * 2000-02-11 2003-09-04 Millennium Pharmaceuticals, Inc. Novel seven-transmembrane proteins/G-protein coupled receptors
JP5051738B2 (ja) 2000-09-20 2012-10-17 イー・エム・デイー・ミリポア・コーポレイシヨン 人工遍在的クロマチン開放エレメント（ｕｃｏｅ）
DE60236334D1 (de) 2001-07-04 2010-06-17 Chromagenics Bv DNS-Sequenzen mit anti-repressor Aktivität
EP1273666A1 (de) 2001-07-04 2003-01-08 Chromagenics B.V. Verfahren zur Selektion einer transkription-modulatierenden DNA-Sequenz unter Verwendung einem Vektor mit einem transkriptions-repressions-aktivem Element
EP1513936A2 (de) * 2002-06-14 2005-03-16 Chromagenics B.V. Verwendung von repression hemmende sequenzen in verfahren zur genexpression verbesserung
CA2489475C (en) 2002-06-14 2011-03-29 Chromagenics B.V. A method for simultaneous production of multiple proteins; vectors and cells for use therein
DK1573058T3 (da) 2002-12-18 2012-01-16 Chromagenics Bv Fremgangsmåde til forbedring af proteinproduktion
EP1572994B1 (de) 2002-12-20 2007-02-21 Chromagenics B.V. Mittel und methoden zur produktion eines proteins durch chromatin-öffner, die chromatin zugänglicher für transkriptionsfaktoren machen können
US7384878B2 (en) * 2004-05-20 2008-06-10 International Business Machines Corporation Method for applying a layer to a hydrophobic surface

Also Published As

Publication number Publication date
US20080227151A1 (en) 2008-09-18
DE60336228D1 (de) 2011-04-14
JP4647309B2 (ja) 2011-03-09
US20050191723A1 (en) 2005-09-01
EP1513937A2 (de) 2005-03-16
DK1513937T3 (da) 2011-05-16
NZ537073A (en) 2006-08-31
EP1513937B1 (de) 2011-03-02
CA2723500C (en) 2014-10-28
CA2722998A1 (en) 2003-12-24
JP5095693B2 (ja) 2012-12-12
JP2010011859A (ja) 2010-01-21
JP2005529610A (ja) 2005-10-06
AU2003238719A1 (en) 2003-12-31
JP2010004886A (ja) 2010-01-14
CA2723500A1 (en) 2003-12-24
CA2489475A1 (en) 2003-12-24
WO2003106684A3 (en) 2004-04-08
WO2003106684A2 (en) 2003-12-24
US7960143B2 (en) 2011-06-14
JP5095690B2 (ja) 2012-12-12
CA2722998C (en) 2014-12-02
AU2003238719B2 (en) 2008-02-21
US7364878B2 (en) 2008-04-29
US20080227199A1 (en) 2008-09-18
ES2362273T3 (es) 2011-06-30
US7901908B2 (en) 2011-03-08
CA2489475C (en) 2011-03-29

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