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ATE435278T1 - Modifizierte nitrilasen und deren verwendung in verfahren zur herstellung von carbonsäuren - Google Patents

Modifizierte nitrilasen und deren verwendung in verfahren zur herstellung von carbonsäuren

Info

Publication number
ATE435278T1
ATE435278T1 AT04713882T AT04713882T ATE435278T1 AT E435278 T1 ATE435278 T1 AT E435278T1 AT 04713882 T AT04713882 T AT 04713882T AT 04713882 T AT04713882 T AT 04713882T AT E435278 T1 ATE435278 T1 AT E435278T1
Authority
AT
Austria
Prior art keywords
nitrilases
acid sequences
nucleic acid
carboxylic acids
expression constructs
Prior art date
Application number
AT04713882T
Other languages
English (en)
Inventor
Thomas Zelinski
Maria Kesseler
Bernhard Hauer
Thomas Friedrich
Original Assignee
Basf Se
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Basf Se filed Critical Basf Se
Application granted granted Critical
Publication of ATE435278T1 publication Critical patent/ATE435278T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/42—Hydroxy-carboxylic acids

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
AT04713882T 2003-02-27 2004-02-24 Modifizierte nitrilasen und deren verwendung in verfahren zur herstellung von carbonsäuren ATE435278T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US45047003P 2003-02-27 2003-02-27
PCT/EP2004/001804 WO2004076655A1 (en) 2003-02-27 2004-02-24 Modified nitrilases and their use in methods for the production of carboxylic acids

Publications (1)

Publication Number Publication Date
ATE435278T1 true ATE435278T1 (de) 2009-07-15

Family

ID=32927657

Family Applications (1)

Application Number Title Priority Date Filing Date
AT04713882T ATE435278T1 (de) 2003-02-27 2004-02-24 Modifizierte nitrilasen und deren verwendung in verfahren zur herstellung von carbonsäuren

Country Status (8)

Country Link
US (1) US7985572B2 (de)
EP (1) EP1599584B1 (de)
JP (1) JP4584242B2 (de)
CN (1) CN100445375C (de)
AT (1) ATE435278T1 (de)
AU (1) AU2004215238B2 (de)
DE (1) DE602004021778D1 (de)
WO (1) WO2004076655A1 (de)

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AT412092B (de) * 2003-02-27 2004-09-27 Dsm Fine Chem Austria Gmbh Verfahren zur herstellung chiraler alpha-hydroxycarbonsäuren durch enzymatische hydrolyse von chiralen cyanhydrinen
EP1966381A2 (de) * 2005-12-20 2008-09-10 Basf Se Verfahren zur herstellung von 5-norbornen-2-carbonsäure aus 5-norbornen-2-carbonitril unter verwendung einer arylacetonitrilase
US9283287B2 (en) 2012-04-02 2016-03-15 Moderna Therapeutics, Inc. Modified polynucleotides for the production of nuclear proteins
EP2834358A4 (de) * 2012-04-02 2016-03-09 Moderna Therapeutics Inc Modifizierte polynukleotide zur herstellung von kernproteinen
US9303079B2 (en) 2012-04-02 2016-04-05 Moderna Therapeutics, Inc. Modified polynucleotides for the production of cytoplasmic and cytoskeletal proteins
CN103898083B (zh) * 2014-04-21 2016-06-29 武汉大学 一种新型水解酶超家族酰胺酶Azl13及其制备与应用
US9804036B2 (en) * 2014-06-19 2017-10-31 Infineon Technologies Ag Temperature sensor calibration
CN105130792A (zh) * 2015-09-21 2015-12-09 上海佰瑞生物科技有限公司 一种对由生物法合成的(r)-(-)-扁桃酸进行分离纯化的方法
CN105349583A (zh) * 2015-12-23 2016-02-24 武汉武药制药有限公司 一种酶法制备(r)-邻氯扁桃酸的方法及应用
CN106854673B (zh) * 2016-12-23 2020-05-15 枣庄市杰诺生物酶有限公司 利用腈水解酶工程菌制备r-邻氯扁桃酸的方法
CN110205315B (zh) * 2019-05-30 2021-01-01 中国石油大学(华东) 腈水解酶XiNit2及其编码基因和应用
CN111471668B (zh) * 2020-02-28 2022-05-24 浙江工业大学 一种腈水解酶突变体及其在制备1-氰基环己基乙酸中的应用
CN113025671B (zh) * 2020-06-23 2023-01-31 浙江恒康药业股份有限公司 苜蓿中华根瘤菌来源的腈水合酶在制备酰胺吡嗪类化合物中的应用

Family Cites Families (25)

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AU611080B2 (en) 1986-01-08 1991-06-06 Rhone-Poulenc Agrochimie Haloarylnitrile degrading gene, its use, and cells containing the same
DE68926922T2 (de) 1988-03-08 1996-12-19 Japan Energy Corp., Tokio/Tokyo Verfahren zur Herstellung von L-alpha-Aminosäuren
US5283193A (en) 1988-06-27 1994-02-01 Asahi Kasei Kogyo K.K. Process for producing optically active α-substituted organic acid and microorganism and enzyme used therefor
DK314989A (da) 1988-06-27 1989-12-28 Asahi Chemical Ind Fremgangsmaade til fremstilling af optisk aktive alfa-substituerede organiske syrer, samt mikroorganismer og enzymer anvendelige ved fremgangsmaaden
DE69131217T2 (de) 1990-03-30 1999-09-23 Mitsubishi Rayon Co., Ltd. Verfahren zur Herstellung von R(-)Mandelsäure und deren Derivaten
JP2974737B2 (ja) 1990-08-16 1999-11-10 三菱レイヨン株式会社 光学活性乳酸の製造法
WO1992005275A1 (en) 1990-09-20 1992-04-02 E.I. Du Pont De Nemours And Company A process for the preparation of enantiomeric 2-alkanoic acids
JP2676568B2 (ja) 1991-06-26 1997-11-17 日東化学工業株式会社 Ｒ（−）−マンデル酸およびその誘導体の製造法
JP2720140B2 (ja) 1993-02-03 1998-02-25 日東化学工業株式会社 フェニル基を有する光学活性α−ヒドロキシカルボン酸の製造法
JP3218133B2 (ja) 1993-02-03 2001-10-15 三菱レイヨン株式会社 フェニル基を有する光学活性α−ヒドロキシカルボン酸の製造法
JP3354688B2 (ja) 1994-01-28 2002-12-09 三菱レイヨン株式会社 微生物によるα−ヒドロキシ酸またはα−ヒドロキシアミドの製造法
RU2144959C1 (ru) 1994-09-22 2000-01-27 Рон-Пуленк Нютрисьон Анималь Ферментативный гидролиз 4-метилтиобутиронитрилов
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KR100540991B1 (ko) 1999-10-26 2006-01-10 쇼와 덴코 가부시키가이샤 신규한 로도코커스속 세균, 로도코커스속 세균 유래의 니트릴라아제 유전자, 니트릴 하이드라타아제 유전자 및 아미다아제 유전자, 및 이들을 이용한 카르복실산의 제조방법
FR2800749B1 (fr) 1999-11-08 2004-07-16 Rhone Poulenc Animal Nutrition Procede de modulation de la selective des nitrilases, nouvelles nitrilases obtenues par ce procede et leur utilisation
US7300775B2 (en) 1999-12-29 2007-11-27 Verenium Corporation Methods for producing α-substituted carboxylic acids using nitrilases and strecker reagents
WO2003000840A2 (en) * 2001-06-21 2003-01-03 Diversa Corporation Nitrilases
DE10010149A1 (de) 2000-03-03 2001-09-06 Basf Ag Nitrilase aus Rhodococcus rhodochrous NCIMB 11216
WO2002029079A2 (en) 2000-10-04 2002-04-11 Maxygen, Inc. Enantioselective production of amino carboxylic acids
WO2002099044A2 (en) 2001-06-05 2002-12-12 Exelixis, Inc. B3galts as modifiers of the p53 pathway and methods of use
AU2003251523A1 (en) 2002-06-13 2003-12-31 Diversa Corporation Processes for making (r)-ethyl 4-cyano-3-hydroxybutyric acid

Also Published As

Publication number Publication date
WO2004076655A1 (en) 2004-09-10
US7985572B2 (en) 2011-07-26
CN100445375C (zh) 2008-12-24
US20060259999A1 (en) 2006-11-16
EP1599584B1 (de) 2009-07-01
AU2004215238A1 (en) 2004-09-10
AU2004215238B2 (en) 2009-08-27
EP1599584A1 (de) 2005-11-30
DE602004021778D1 (de) 2009-08-13
CN1753990A (zh) 2006-03-29
JP2006519002A (ja) 2006-08-24
JP4584242B2 (ja) 2010-11-17

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