Nothing Special   »   [go: up one dir, main page]

ATE428779T1 - Verfahren zur ortspezifischen massenmutagenese - Google Patents

Verfahren zur ortspezifischen massenmutagenese

Info

Publication number
ATE428779T1
ATE428779T1 AT04292987T AT04292987T ATE428779T1 AT E428779 T1 ATE428779 T1 AT E428779T1 AT 04292987 T AT04292987 T AT 04292987T AT 04292987 T AT04292987 T AT 04292987T AT E428779 T1 ATE428779 T1 AT E428779T1
Authority
AT
Austria
Prior art keywords
library
mutagenesis
generating
site
specific mass
Prior art date
Application number
AT04292987T
Other languages
English (en)
Inventor
Julien Sylvestre
Marc Delcourt
Original Assignee
Biomethodes
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biomethodes filed Critical Biomethodes
Application granted granted Critical
Publication of ATE428779T1 publication Critical patent/ATE428779T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
AT04292987T 2003-12-18 2004-12-14 Verfahren zur ortspezifischen massenmutagenese ATE428779T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR0314892 2003-12-18

Publications (1)

Publication Number Publication Date
ATE428779T1 true ATE428779T1 (de) 2009-05-15

Family

ID=34508693

Family Applications (1)

Application Number Title Priority Date Filing Date
AT04292987T ATE428779T1 (de) 2003-12-18 2004-12-14 Verfahren zur ortspezifischen massenmutagenese

Country Status (4)

Country Link
US (1) US20050153343A1 (de)
EP (1) EP1544296B8 (de)
AT (1) ATE428779T1 (de)
DE (1) DE602004020570D1 (de)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060199250A1 (en) * 2005-03-04 2006-09-07 Huimin Zhao Method for generating a mutant protein which efficiently binds a target molecule
AU2006315347A1 (en) * 2005-11-16 2007-05-24 Ambrx, Inc. Methods and compositions comprising non-natural amino acids
KR100784478B1 (ko) * 2005-12-05 2007-12-11 한국과학기술원 기능요소의 동시 삽입에 의한 신기능을 갖는 단백질을제조하는 방법
US20080287320A1 (en) * 2006-10-04 2008-11-20 Codon Devices Libraries and their design and assembly
EP2928913A1 (de) * 2012-12-10 2015-10-14 Fred Hutchinson Cancer Research Center Lipocalin-fusionspartner
JP2023509618A (ja) * 2019-12-30 2023-03-09 ナンジン、ジェンスクリプト、バイオテック、カンパニー、リミテッド 遺伝子変異ライブラリーを構築する方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6479258B1 (en) * 1995-12-07 2002-11-12 Diversa Corporation Non-stochastic generation of genetic vaccines
US20030186226A1 (en) * 1999-03-08 2003-10-02 Brennan Thomas M. Methods and compositions for economically synthesizing and assembling long DNA sequences
FR2813314B1 (fr) * 2000-08-25 2004-05-07 Biomethodes Procede de mutagenese dirigee massive
FR2826957B1 (fr) * 2001-07-09 2005-09-30 Centre Nat Rech Scient Procede de fonctionnalisation de supports solides, supports solides fonctionnalises et leurs utilisations
US20030219781A1 (en) * 2002-01-14 2003-11-27 Diversa Corporation Compositions and methods for making polynucleotides by iterative assembly of codon building blocks

Also Published As

Publication number Publication date
EP1544296B8 (de) 2009-08-05
EP1544296A1 (de) 2005-06-22
US20050153343A1 (en) 2005-07-14
DE602004020570D1 (de) 2009-05-28
EP1544296B1 (de) 2009-04-15

Similar Documents

Publication Publication Date Title
JP2015522286A5 (de)
DK1328627T3 (da) Fremgangsmåde til dannelse af et bibliotek over oligonukleotider, der omfatter en styret fordeling af mutationer
ATE388228T1 (de) Verfahren zur verknüpfung interessierender sequenzen
WO2012074130A1 (ja) ペプチドライブラリーの製造方法、ペプチドライブラリー、及びスクリーニング方法
ATE439380T1 (de) Methoden zur herstellung humaner monoklonaler antikörper
EP1075513B8 (de) Kontinuierliche in vitro-evolution.
HUP0301723A2 (hu) Eljárás peptidek előállítására in vitro transzkripciós/transzlációs rendszer alkalmazásával
WO2004050899A3 (en) Method for in vitro selection of 2’-substituted nucleic acids
WO2005010150A3 (en) Method for in vitro selection of 2’-substituted nucleic acids
JP2010535150A5 (de)
WO2003075129A3 (en) Methods, systems, and software for identifying functional bio-molecules
DK0988378T3 (da) En fremgangsmåde til in vitro molekylær udvikling af proteinfunktion
EA200500920A1 (ru) Стабильный пенистый крем
WO2005051970A3 (de) Hochparalleler dna-synthesizer auf matrizenbasis
CA2879834A1 (en) Highly diverse combinatorial antibody libraries
ATE428779T1 (de) Verfahren zur ortspezifischen massenmutagenese
WO2004072261A3 (en) Sirna libraries optimized for predetermined protein families
DK1948794T3 (da) Fremgangsmåde til molekylær evolution in vitro af proteinfunktion
ATE474936T1 (de) Schnelle synthese von oligonukleotiden
WO2004046340A3 (en) Intron fusion construct and method of using for selecting high-expressing production cell lines
ATE278702T1 (de) Verfahren zur synthese von phosphorothioat- oligonukelotiden
DE60326224D1 (de) Signaturen konstanter länge für das parallele sequenzieren von polynukleotiden
WO2005003151A3 (en) Method and apparatus for continuous large-scale radiolabeling of proteins
WO2005023985A3 (en) Cytidine deaminase activators, deoxycytidine deaminase activators, vif antagonists, and methods of screening for molecules thereof
Marinov et al. ChIP-ping the branches of the tree: functional genomics and the evolution of eukaryotic gene regulation

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties