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ATE497018T1 - Materialien und methoden zum nachweis von nukleinsäuren - Google Patents

Materialien und methoden zum nachweis von nukleinsäuren

Info

Publication number
ATE497018T1
ATE497018T1 AT01950231T AT01950231T ATE497018T1 AT E497018 T1 ATE497018 T1 AT E497018T1 AT 01950231 T AT01950231 T AT 01950231T AT 01950231 T AT01950231 T AT 01950231T AT E497018 T1 ATE497018 T1 AT E497018T1
Authority
AT
Austria
Prior art keywords
target nucleic
sample
stranded region
nucleic acids
nucleic acid
Prior art date
Application number
AT01950231T
Other languages
English (en)
Inventor
David J Marshall
James R Prudent
Christopher B Scherrill
Gideon Shapiro
Jennifer K Grenier
Craig S Richmond
Simona Jurczyk
Jerod L Ptacin
Original Assignee
Eragen Biosciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eragen Biosciences Inc filed Critical Eragen Biosciences Inc
Application granted granted Critical
Publication of ATE497018T1 publication Critical patent/ATE497018T1/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Saccharide Compounds (AREA)
  • Catalysts (AREA)
  • Crystals, And After-Treatments Of Crystals (AREA)
  • Weting (AREA)
AT01950231T 2000-05-19 2001-05-18 Materialien und methoden zum nachweis von nukleinsäuren ATE497018T1 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US20571200P 2000-05-19 2000-05-19
US24039800P 2000-10-14 2000-10-14
US28283101P 2001-04-10 2001-04-10
PCT/US2001/016359 WO2001090417A2 (en) 2000-05-19 2001-05-18 Materials and methods for detection of nucleic acids

Publications (1)

Publication Number Publication Date
ATE497018T1 true ATE497018T1 (de) 2011-02-15

Family

ID=27394838

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01950231T ATE497018T1 (de) 2000-05-19 2001-05-18 Materialien und methoden zum nachweis von nukleinsäuren

Country Status (10)

Country Link
US (6) US7422850B2 (de)
EP (1) EP1349954B1 (de)
JP (2) JP5138141B2 (de)
CN (1) CN1592792B (de)
AT (1) ATE497018T1 (de)
AU (1) AU7125401A (de)
CA (1) CA2409309A1 (de)
DE (1) DE60143960D1 (de)
HK (1) HK1061410A1 (de)
WO (1) WO2001090417A2 (de)

Families Citing this family (63)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2409309A1 (en) * 2000-05-19 2001-11-29 Eragen Biosciences, Inc. Materials and methods for detection of nucleic acids
WO2002033126A2 (en) 2000-10-14 2002-04-25 Eragen Biosciences, Inc. Solid support assay systems and methods utilizing non-standard bases
EP1236804A1 (de) * 2001-03-02 2002-09-04 Boehringer Mannheim Gmbh Verfahren zur Bestimmung von Nukleinsäuren mittels einer Kontrolle
WO2003074724A2 (en) * 2002-03-01 2003-09-12 Integrated Dna Technologies, Inc. Polynomial amplification of nucleic acids
CA2513626C (en) * 2003-01-17 2013-03-19 Eragen Biosciences, Inc. Nucleic acid amplification using non-standard bases
US7820808B2 (en) 2003-04-01 2010-10-26 Eragen Biosciences, Inc. Polymerase inhibitor and method of using same
CN1280428C (zh) * 2003-05-19 2006-10-18 清华大学 一种基于微小颗粒的生物芯片系统及其应用
US7282333B2 (en) * 2003-10-20 2007-10-16 Promega Corporation Methods and compositions for nucleic acid analysis
WO2005081776A2 (en) * 2004-01-30 2005-09-09 Eragen Biosciences, Inc. Materials and methods for the detection of sars
EP1733054A2 (de) * 2004-04-06 2006-12-20 Epigenomics AG Verfahren zur quantifizierung methylierter dna
US20060172324A1 (en) * 2005-01-28 2006-08-03 Roche Molecular Systems, Inc. Methods of genotyping using differences in melting temperature
WO2006095550A1 (ja) * 2005-03-04 2006-09-14 Kyoto University Ｐｃｒプライマー、それを利用したｐｃｒ法及びｐｃｒ増幅産物、並びにｐｃｒ増幅産物を利用するデバイス及びｄｎａ-タンパク複合体
US7498136B2 (en) * 2005-03-18 2009-03-03 Eragen Biosciences, Inc. Methods for detecting multiple species and subspecies of Neisseria
US8293472B2 (en) 2005-06-07 2012-10-23 Luminex Corporation Methods for detection and typing of nucleic acids
US20120122095A1 (en) * 2006-01-12 2012-05-17 Eragen Biosciences, Inc. Materials and methods for the detection of anthrax related toxin genes
US20070264694A1 (en) * 2006-04-07 2007-11-15 Eragen Biosciences, Inc. Use of non-standard bases and proximity effects for gene assembly and conversion of non-standard bases to standard bases during dna synthesis
US20080153097A1 (en) * 2006-11-15 2008-06-26 Eragen Biosciences, Inc. Methods and kits for detecting jak2 nucleic acid
US20080299568A1 (en) * 2007-04-27 2008-12-04 Scott Johnson Materials and methods for detection of hepatitis c virus
US7678894B2 (en) * 2007-05-18 2010-03-16 Helicos Biosciences Corporation Nucleotide analogs
US9163053B2 (en) 2007-05-18 2015-10-20 Fluidigm Corporation Nucleotide analogs
JP2009178159A (ja) * 2007-11-05 2009-08-13 Hitachi Plant Technologies Ltd 核酸配列の検出方法及び核酸配列検出基板
KR100978215B1 (ko) * 2008-02-28 2010-08-26 주식회사 인트론바이오테크놀로지 건조형태의 ｐｃｒ 반응용 조성물에 기반한 ｐｃｒ의 최적증폭 조건 탐색 및 설정에 활용될 수 있는 키트 및 그것의제조방법
US9249455B2 (en) * 2008-04-18 2016-02-02 Luminex Corporation Methods for detection and quantification of small RNA
EP2133434A1 (de) 2008-06-12 2009-12-16 Institut National de la Santé et de la Recherche Médicale (INSERM) Verfahren zum Nachweis von Zielnukleinsäuren
JP5596684B2 (ja) * 2008-08-12 2014-09-24 エフ．ホフマン−ラ ロシュ アーゲー プルーフリーディング・プライマー伸長
EP2331703A2 (de) * 2008-09-12 2011-06-15 Promega Corporation Beurteilen der expression endogener und exogener gene
US20100112565A1 (en) * 2008-11-03 2010-05-06 Life Technologies Corporation Methods, kits, and reaction mixtures for high resolution melt genotyping
US20100112557A1 (en) * 2008-11-03 2010-05-06 Applied Biosystems Inc. Method for high resolution melt genotyping
JP5715068B2 (ja) 2009-01-30 2015-05-07 マイクロニクス， インコーポレイテッド 携帯型高利得蛍光検出システム
JP5938348B2 (ja) * 2009-10-23 2016-06-22 ルミネックス コーポレーション 反応特異性の増加のための非標準塩基を含む増幅プライマー
US8524450B2 (en) 2009-10-30 2013-09-03 Illumina, Inc. Microvessels, microparticles, and methods of manufacturing and using the same
US9758817B2 (en) * 2010-01-13 2017-09-12 Agilent Technologies, Inc. Method for identifying a nucleic acid in a sample
CA2786569C (en) 2010-01-29 2019-04-09 Micronics, Inc. Sample-to-answer microfluidic cartridge
EP2556458B1 (de) * 2010-04-09 2020-06-03 Life Technologies Corporation Visualisierungswerkzeug für qpcr-genotypisierungs-daten
MX340258B (es) * 2011-01-11 2016-07-01 Seegene Inc Detección de secuencias de ácido nucleico objetivo mediante ensayo de escisión y extensión del pto.
KR20130101952A (ko) * 2012-02-02 2013-09-16 주식회사 씨젠 Ｐｔｏ 절단과 연장-의존적 혼성화를 이용한 타겟 핵산서열의 검출
ES2669244T3 (es) 2012-03-05 2018-05-24 Seegene, Inc. Detección de variación de nucleótidos en una secuencia de ácido nucleico diana mediante escisión CTO y ensayo de extensión
AU2013202808B2 (en) 2012-07-31 2014-11-13 Gen-Probe Incorporated System and method for performing multiplex thermal melt analysis
JP6498125B2 (ja) 2012-12-21 2019-04-10 マイクロニクス， インコーポレイテッド 流体回路および関連する製造方法
CN104994957B (zh) 2012-12-21 2017-11-28 精密公司 用于微流体用途的低弹性膜
JP2016509206A (ja) 2012-12-21 2016-03-24 マイクロニクス， インコーポレイテッド 携帯型蛍光検出システムおよびマイクロアッセイカートリッジ
EP2770065B1 (de) 2013-02-25 2017-12-13 Seegene, Inc. Detektion einer Nukleotidvariation auf Nukleinsäurezielsequenz
AU2013202788B2 (en) 2013-03-14 2015-10-01 Gen-Probe Incorporated Indexing signal detection module
EP2994543B1 (de) 2013-05-07 2018-08-15 Micronics, Inc. Vorrichtung zur herstellung und analyse von nukleinsäuren
WO2014182831A1 (en) 2013-05-07 2014-11-13 Micronics, Inc. Methods for preparation of nucleic acid-containing samples using clay minerals and alkaline solutions
EP2994750B1 (de) 2013-05-07 2020-08-12 PerkinElmer Health Sciences, Inc. Mikrofluidische vorrichtungen und verfahren zur durchführung von serumtrennung und blutkreuzproben
EP3022319B1 (de) 2013-07-15 2023-05-17 Seegene, Inc. Erkennung einer nukleinsäurezielsequenz durch pto-spaltung und erweiterungsabhängige immobilisierte oligonukleotidhybridisierung
CN113528621A (zh) 2013-08-09 2021-10-22 卢米耐克斯公司 用于在核酸测定中改善熔链分辨和多重性的探针
JP6295322B2 (ja) 2013-10-18 2018-03-14 シージーン アイエヌシー ｈＣＴＯを利用するＰＴＯ切断及び延長分析による固相におけるターゲット核酸配列の検出
WO2015147370A1 (en) 2014-03-28 2015-10-01 Seegene, Inc. Detection of target nucleic acid sequences using different detection temperatures
EP4299760A3 (de) * 2014-08-11 2024-02-21 Luminex Corporation Sonden für verbesserte schmelzunterscheidung und multiplexierung in nukleinsäuretests
US11915795B2 (en) * 2016-12-23 2024-02-27 The Regents Of The University Of California Method and device for digital high resolution melt
CN106950326B (zh) * 2017-03-10 2018-05-29 武汉大学 一种化学标记结合lc-ms的方法及其在核苷酸分析中的应用
US11193153B2 (en) 2017-03-20 2021-12-07 President And Fellows Of Harvard College Programmable nucleic acid synthesis cascade
US10626452B2 (en) * 2017-09-29 2020-04-21 Sekisui Medical Co., Ltd. Method for detecting single base substitution using ion-exchange chromatography
WO2019066461A2 (en) 2017-09-29 2019-04-04 Seegene, Inc. DETECTION OF TARGET NUCLEIC ACID SEQUENCES BY CLEAVAGE ANALYSIS AND EXTENSION OF PTO
WO2019178337A1 (en) 2018-03-14 2019-09-19 Luminex Corporation Multiplex amplification of nucleic acids employing lowering of denaturation temperature and increasing the annealing temperature
EP3781708A4 (de) 2018-04-20 2022-01-05 Seegene, Inc. Verfahren und vorrichtung zur detektion einer vielzahl von zielnukleinsäuresequenzen in einer probe
WO2020014296A1 (en) 2018-07-12 2020-01-16 Luminex Corporation Systems and methods for performing variable sample preparation and analysis processes
US11891662B2 (en) * 2019-12-02 2024-02-06 Talis Biomedical Corporation Polynucleotides for amplification and detection of human beta actin
CN113913497B (zh) * 2021-02-03 2024-05-28 山东舜丰生物科技有限公司 利用碱基修饰的单链核酸进行靶核酸检测的方法
KR20240058112A (ko) 2021-09-17 2024-05-03 주식회사 씨젠 합성 비자연 염기를 포함하는 태그 올리고뉴클레오타이드를 이용한 타겟 핵산 서열의 검출
WO2024181774A1 (en) * 2023-02-28 2024-09-06 Seegene, Inc. Detection of target nucleic acid by lpho-assisted pto cleavage and extension assay

Family Cites Families (54)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4458066A (en) 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4996143A (en) * 1985-12-23 1991-02-26 Syngene, Inc. Fluorescent stokes shift probes for polynucleotide hybridization
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
GB8827160D0 (en) 1988-11-21 1988-12-29 Apothekernes Lab Detection & quantitative determination of rna & dna
GB8902689D0 (en) 1989-02-07 1989-03-30 Ici Plc Assay method
US5856092A (en) * 1989-02-13 1999-01-05 Geneco Pty Ltd Detection of a nucleic acid sequence or a change therein
NZ235032A (en) 1989-08-31 1993-04-28 Dow Chemical Co Constrained geometry complexes of titanium, zirconium or hafnium comprising a substituted cyclopentadiene ligand; use as olefin polymerisation catalyst component
GB8920097D0 (en) * 1989-09-06 1989-10-18 Ici Plc Amplification processes
US6007984A (en) 1989-11-01 1999-12-28 Zeneca Limited Detection of DNA/RNA by fluorescence polarization
US5200314A (en) 1990-03-23 1993-04-06 Chiron Corporation Polynucleotide capture assay employing in vitro amplification
US5210015A (en) * 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5965364A (en) * 1990-10-09 1999-10-12 Benner; Steven Albert Method for selecting functional deoxyribonucleotide derivatives
US6140496A (en) 1990-10-09 2000-10-31 Benner; Steven Albert Precursors for deoxyribonucleotides containing non-standard nucleosides
US6037120A (en) 1995-10-12 2000-03-14 Benner; Steven Albert Recognition of oligonucleotides containing non-standard base pairs
US5432272A (en) 1990-10-09 1995-07-11 Benner; Steven A. Method for incorporating into a DNA or RNA oligonucleotide using nucleotides bearing heterocyclic bases
US5994056A (en) * 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
DE4129653A1 (de) * 1991-09-06 1993-03-11 Boehringer Mannheim Gmbh Verfahren zum nachweis aehnlicher nukleinsaeuren
US5846717A (en) * 1996-01-24 1998-12-08 Third Wave Technologies, Inc. Detection of nucleic acid sequences by invader-directed cleavage
WO1994021820A1 (en) 1993-03-17 1994-09-29 City Of Hope Nucleic acid sequences having single stranded ends
US6077668A (en) * 1993-04-15 2000-06-20 University Of Rochester Highly sensitive multimeric nucleic acid probes
US5654138A (en) 1993-05-14 1997-08-05 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Von hippel-lindau (VHL) disease gene and corresponding cDNA and methods for detecting carriers of the VHL disease gene
US6156501A (en) 1993-10-26 2000-12-05 Affymetrix, Inc. Arrays of modified nucleic acid probes and methods of use
US5589329A (en) 1993-11-15 1996-12-31 Ambion, Inc. Methods and compositions for detecting base pair mismatches
ATE247716T1 (de) * 1994-02-07 2003-09-15 Beckman Coulter Inc Ligase/polymerase-vermittelte analyse genetischer elemente von einzelnukleotid-polymorphismen und ihre verwendung in der genetischen analyse
US5681702A (en) * 1994-08-30 1997-10-28 Chiron Corporation Reduction of nonspecific hybridization by using novel base-pairing schemes
US5604097A (en) * 1994-10-13 1997-02-18 Spectragen, Inc. Methods for sorting polynucleotides using oligonucleotide tags
US5866337A (en) 1995-03-24 1999-02-02 The Trustees Of Columbia University In The City Of New York Method to detect mutations in a nucleic acid using a hybridization-ligation procedure
GB9507238D0 (en) 1995-04-07 1995-05-31 Isis Innovation Detecting dna sequence variations
US5736330A (en) * 1995-10-11 1998-04-07 Luminex Corporation Method and compositions for flow cytometric determination of DNA sequences
US5980861A (en) 1996-03-12 1999-11-09 University Of Massachusetts Chelator compositions and methods of synthesis thereof
WO1997035033A1 (en) * 1996-03-19 1997-09-25 Molecular Tool, Inc. Method for determining the nucleotide sequence of a polynucleotide
US5780233A (en) 1996-06-06 1998-07-14 Wisconsin Alumni Research Foundation Artificial mismatch hybridization
US5866336A (en) * 1996-07-16 1999-02-02 Oncor, Inc. Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US6124092A (en) 1996-10-04 2000-09-26 The Perkin-Elmer Corporation Multiplex polynucleotide capture methods and compositions
US5853993A (en) * 1996-10-21 1998-12-29 Hewlett-Packard Company Signal enhancement method and kit
JP4185185B2 (ja) * 1997-05-08 2008-11-26 征夫 軽部 部分二重鎖ｄｎａを利用したｄｎａの検出方法
US5928869A (en) * 1997-05-30 1999-07-27 Becton, Dickinson And Company Detection of nucleic acids by fluorescence quenching
IL135852A0 (en) * 1997-10-28 2001-05-20 Univ California A method for detecting dna base mismatch
GB9725197D0 (en) * 1997-11-29 1998-01-28 Secr Defence Detection system
US6046807A (en) * 1998-05-14 2000-04-04 Luminex Corporation Diode laser based measurement apparatus
US7060431B2 (en) * 1998-06-24 2006-06-13 Illumina, Inc. Method of making and decoding of array sensors with microspheres
EP1141414A4 (de) 1998-12-30 2005-03-02 Dana Farber Cancer Inst Inc Mutationsdurchmusterungsarray und methoden zu dessen verwendung
US6432642B1 (en) * 1999-01-15 2002-08-13 Pe Corporation (Ny) Binary probe and clamp composition and methods for a target hybridization detection
US6528254B1 (en) * 1999-10-29 2003-03-04 Stratagene Methods for detection of a target nucleic acid sequence
JP2001204497A (ja) 2000-01-20 2001-07-31 Japan Science & Technology Corp 蛍光共鳴エネルギー移動を利用した、ｄｎａハイブリダイゼーション法によるｄｎａ検出方法
US6783985B1 (en) 2000-02-18 2004-08-31 Elitra Pharmaceuticals Inc. Gene disruption methodologies for drug target discovery
CA2409309A1 (en) * 2000-05-19 2001-11-29 Eragen Biosciences, Inc. Materials and methods for detection of nucleic acids
US6511809B2 (en) * 2000-06-13 2003-01-28 E. I. Du Pont De Nemours And Company Method for the detection of an analyte by means of a nucleic acid reporter
WO2002033126A2 (en) * 2000-10-14 2002-04-25 Eragen Biosciences, Inc. Solid support assay systems and methods utilizing non-standard bases
US6843657B2 (en) * 2001-01-12 2005-01-18 Litton Systems Inc. High speed, high density interconnect system for differential and single-ended transmission applications
GB0129450D0 (en) * 2001-12-08 2002-01-30 Koninkl Philips Electronics Nv Trenched semiconductor devices and their manufacture
US20030194705A1 (en) 2002-03-01 2003-10-16 Schroth Gary P. Methods of using unnatural nucleobases for decoding

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US20090226926A1 (en) 2009-09-10
JP5138141B2 (ja) 2013-02-06
US7517651B2 (en) 2009-04-14
US20100291570A1 (en) 2010-11-18
DE60143960D1 (de) 2011-03-10
US20070031885A1 (en) 2007-02-08
WO2001090417A2 (en) 2001-11-29
JP5685561B2 (ja) 2015-03-18
AU7125401A (en) 2001-12-03
HK1061410A1 (en) 2004-09-17
US7541147B2 (en) 2009-06-02
CN1592792A (zh) 2005-03-09
EP1349954A2 (de) 2003-10-08
US20140057269A1 (en) 2014-02-27
JP2013005803A (ja) 2013-01-10
JP2004500850A (ja) 2004-01-15
US7422850B2 (en) 2008-09-09
US9611509B2 (en) 2017-04-04
EP1349954B1 (de) 2011-01-26
US20060252091A1 (en) 2006-11-09
CN1592792B (zh) 2010-12-01
US20020150900A1 (en) 2002-10-17
WO2001090417A3 (en) 2003-08-07
CA2409309A1 (en) 2001-11-29

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