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ATE496998T1 - Verfahren zur fehlerverringerung in nukleinsäurepopulationen - Google Patents

Verfahren zur fehlerverringerung in nukleinsäurepopulationen

Info

Publication number
ATE496998T1
ATE496998T1 AT05810178T AT05810178T ATE496998T1 AT E496998 T1 ATE496998 T1 AT E496998T1 AT 05810178 T AT05810178 T AT 05810178T AT 05810178 T AT05810178 T AT 05810178T AT E496998 T1 ATE496998 T1 AT E496998T1
Authority
AT
Austria
Prior art keywords
dna
segments
microarray
substrate
nucleic acid
Prior art date
Application number
AT05810178T
Other languages
English (en)
Inventor
Peter Belshaw
Michael Sussman
Franco Cerrina
James Kaysen
Brock Binkowski
Karhryn Richmond
Original Assignee
Wisconsin Alumni Res Found
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wisconsin Alumni Res Found filed Critical Wisconsin Alumni Res Found
Application granted granted Critical
Publication of ATE496998T1 publication Critical patent/ATE496998T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
AT05810178T 2004-08-27 2005-08-29 Verfahren zur fehlerverringerung in nukleinsäurepopulationen ATE496998T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US60486704P 2004-08-27 2004-08-27
PCT/US2005/030831 WO2006026614A2 (en) 2004-08-27 2005-08-29 Method of error reduction in nucleic acid populations

Publications (1)

Publication Number Publication Date
ATE496998T1 true ATE496998T1 (de) 2011-02-15

Family

ID=35613709

Family Applications (1)

Application Number Title Priority Date Filing Date
AT05810178T ATE496998T1 (de) 2004-08-27 2005-08-29 Verfahren zur fehlerverringerung in nukleinsäurepopulationen

Country Status (8)

Country Link
US (1) US7820412B2 (de)
EP (1) EP1781786B1 (de)
JP (1) JP2008517586A (de)
AT (1) ATE496998T1 (de)
AU (1) AU2005279931B2 (de)
CA (1) CA2578564C (de)
DE (1) DE602005026128D1 (de)
WO (1) WO2006026614A2 (de)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7563600B2 (en) 2002-09-12 2009-07-21 Combimatrix Corporation Microarray synthesis and assembly of gene-length polynucleotides
US20070196834A1 (en) * 2005-09-09 2007-08-23 Francesco Cerrina Method and system for the generation of large double stranded DNA fragments
CA2642514C (en) * 2005-12-02 2011-06-07 Lei Young Synthesis of error-minimized nucleic acid molecules
US20070231805A1 (en) * 2006-03-31 2007-10-04 Baynes Brian M Nucleic acid assembly optimization using clamped mismatch binding proteins
WO2007136834A2 (en) * 2006-05-19 2007-11-29 Codon Devices, Inc. Combined extension and ligation for nucleic acid assembly
WO2007136736A2 (en) * 2006-05-19 2007-11-29 Codon Devices, Inc. Methods for nucleic acid sorting and synthesis
WO2008027558A2 (en) * 2006-08-31 2008-03-06 Codon Devices, Inc. Iterative nucleic acid assembly using activation of vector-encoded traits
US8808986B2 (en) 2008-08-27 2014-08-19 Gen9, Inc. Methods and devices for high fidelity polynucleotide synthesis
WO2011056872A2 (en) 2009-11-03 2011-05-12 Gen9, Inc. Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly
WO2011066186A1 (en) 2009-11-25 2011-06-03 Gen9, Inc. Methods and apparatuses for chip-based dna error reduction
WO2011066185A1 (en) 2009-11-25 2011-06-03 Gen9, Inc. Microfluidic devices and methods for gene synthesis
WO2011085075A2 (en) 2010-01-07 2011-07-14 Gen9, Inc. Assembly of high fidelity polynucleotides
US10457935B2 (en) 2010-11-12 2019-10-29 Gen9, Inc. Protein arrays and methods of using and making the same
ES2548400T3 (es) 2010-11-12 2015-10-16 Gen9, Inc. Métodos y dispositivos para la síntesis de ácidos nucleicos
US9752176B2 (en) 2011-06-15 2017-09-05 Ginkgo Bioworks, Inc. Methods for preparative in vitro cloning
DK3594340T3 (da) 2011-08-26 2021-09-20 Gen9 Inc Sammensætninger og fremgangsmåder til samling med høj nøjagtighed af nukleinsyrer
EP2768607B1 (de) 2011-09-26 2021-08-18 Thermo Fisher Scientific GENEART GmbH Multiwell-platte für die hocheffiziente kleinvolumige nukleinsäuresynthese
WO2014153188A2 (en) 2013-03-14 2014-09-25 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
US20130274129A1 (en) 2012-04-04 2013-10-17 Geneart Ag Tal-effector assembly platform, customized services, kits and assays
WO2013163263A2 (en) 2012-04-24 2013-10-31 Gen9, Inc. Methods for sorting nucleic acids and multiplexed preparative in vitro cloning
LT2864531T (lt) 2012-06-25 2019-03-12 Gen9, Inc. Nukleorūgšties konstravimo ir aukšto produktyvumo sekvenavimo būdai
CA2885058A1 (en) * 2012-10-08 2014-04-17 Spiral Genetics Inc. Methods and systems for identifying, from read symbol sequences, variations with respect to a reference symbol sequence
US20160017394A1 (en) 2014-07-15 2016-01-21 Life Technologies Corporation Compositions and methods for nucleic acid assembly
CN115927293A (zh) 2014-12-09 2023-04-07 生命技术公司 高效小体积核酸合成
EP3320103A1 (de) 2015-07-07 2018-05-16 Thermo Fisher Scientific Geneart GmbH Enzymatische synthese von nukleinsäuresequenzen
EP3763818A1 (de) 2015-10-06 2021-01-13 Pierce Biotechnology, Inc. Vorrichtungen und verfahren zur herstellung von proteinen
GB2566986A (en) 2017-09-29 2019-04-03 Evonetix Ltd Error detection during hybridisation of target double-stranded nucleic acid

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6027877A (en) * 1993-11-04 2000-02-22 Gene Check, Inc. Use of immobilized mismatch binding protein for detection of mutations and polymorphisms, purification of amplified DNA samples and allele identification
ES2344772T3 (es) * 1998-02-23 2010-09-06 Wisconsin Alumni Research Foundation Metodo y aparato para la sintesis de matrices de sondas de adn.
GB9808145D0 (en) * 1998-04-17 1998-06-17 Zeneca Ltd Assay
US6991922B2 (en) * 1998-08-12 2006-01-31 Proteus S.A. Process for in vitro creation of recombinant polynucleotide sequences by oriented ligation
WO2002095073A1 (en) * 2001-05-18 2002-11-28 Wisconsin Alumni Research Foundation Method for the synthesis of dna sequences
WO2003054232A2 (en) * 2001-12-13 2003-07-03 Blue Heron Biotechnology, Inc. Methods for removal of double-stranded oligonucleotides containing sequence errors using mismatch recognition proteins
AU2003213696B2 (en) * 2002-02-28 2008-01-24 Wisconsin Alumni Research Foundation Method of error reduction in nucleic acid populations
US7879580B2 (en) 2002-12-10 2011-02-01 Massachusetts Institute Of Technology Methods for high fidelity production of long nucleic acid molecules
US7932025B2 (en) 2002-12-10 2011-04-26 Massachusetts Institute Of Technology Methods for high fidelity production of long nucleic acid molecules with error control
EP1613776A1 (de) 2003-04-02 2006-01-11 Blue Heron Biotechnology, Inc. Fehlerreduktion bei der automatisierten gensynthese

Also Published As

Publication number Publication date
JP2008517586A (ja) 2008-05-29
CA2578564A1 (en) 2006-03-09
CA2578564C (en) 2013-05-28
US7820412B2 (en) 2010-10-26
WO2006026614A2 (en) 2006-03-09
WO2006026614A3 (en) 2006-07-06
AU2005279931B2 (en) 2011-02-10
AU2005279931A1 (en) 2006-03-09
DE602005026128D1 (de) 2011-03-10
EP1781786B1 (de) 2011-01-26
US20060127926A1 (en) 2006-06-15
EP1781786A2 (de) 2007-05-09

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