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ATE380881T1 - Verfahren zur entfernung von nukleinsäureverunreinigungen in amplifizierungsreaktionen - Google Patents

Verfahren zur entfernung von nukleinsäureverunreinigungen in amplifizierungsreaktionen

Info

Publication number
ATE380881T1
ATE380881T1 AT98937684T AT98937684T ATE380881T1 AT E380881 T1 ATE380881 T1 AT E380881T1 AT 98937684 T AT98937684 T AT 98937684T AT 98937684 T AT98937684 T AT 98937684T AT E380881 T1 ATE380881 T1 AT E380881T1
Authority
AT
Austria
Prior art keywords
nucleic acid
amplification reaction
removal
amplification reactions
acid impurities
Prior art date
Application number
AT98937684T
Other languages
English (en)
Inventor
Inge Nilsen
Erling Sandsdalen
Even Stenberg
Original Assignee
Norwegian Inst Of Fisheries An
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Norwegian Inst Of Fisheries An filed Critical Norwegian Inst Of Fisheries An
Application granted granted Critical
Publication of ATE380881T1 publication Critical patent/ATE380881T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AT98937684T 1997-08-06 1998-08-06 Verfahren zur entfernung von nukleinsäureverunreinigungen in amplifizierungsreaktionen ATE380881T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GBGB9716664.9A GB9716664D0 (en) 1997-08-06 1997-08-06 A method of removing nucleic acid contamination reactions

Publications (1)

Publication Number Publication Date
ATE380881T1 true ATE380881T1 (de) 2007-12-15

Family

ID=10817101

Family Applications (1)

Application Number Title Priority Date Filing Date
AT98937684T ATE380881T1 (de) 1997-08-06 1998-08-06 Verfahren zur entfernung von nukleinsäureverunreinigungen in amplifizierungsreaktionen

Country Status (8)

Country Link
US (1) US6541204B2 (de)
EP (1) EP1003913B1 (de)
JP (1) JP3801865B2 (de)
AT (1) ATE380881T1 (de)
AU (1) AU749882B2 (de)
DE (1) DE69838839T2 (de)
GB (1) GB9716664D0 (de)
WO (1) WO1999007887A2 (de)

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US20080064108A1 (en) * 1997-12-10 2008-03-13 Tony Baker Urine Preservation System
US7569342B2 (en) * 1997-12-10 2009-08-04 Sierra Molecular Corp. Removal of molecular assay interferences
US20020012916A1 (en) * 1998-10-14 2002-01-31 Gundling Gerard J A method of reducing contamination in an essay vessel
US8361712B2 (en) * 2007-12-17 2013-01-29 General Electric Company Contamination-free reagents for nucleic acid amplification
US8507662B2 (en) 2001-01-19 2013-08-13 General Electric Company Methods and kits for reducing non-specific nucleic acid amplification
AUPS064202A0 (en) * 2002-02-20 2002-03-14 Biomolecular Research Solutions Pty Ltd Improved method for the analysis of nucleic acid samples
AU2003205424B2 (en) * 2002-02-20 2008-11-20 Biomolecular Research Solutions Pty Ltd Removal of nucleic acid contaminants
ATE387494T1 (de) * 2002-12-20 2008-03-15 Hoffmann La Roche Hitzelabile desoxyribonuklease i-varianten
US9487823B2 (en) 2002-12-20 2016-11-08 Qiagen Gmbh Nucleic acid amplification
EP1431387B1 (de) * 2002-12-20 2008-02-27 Roche Diagnostics GmbH Hitzelabile Desoxyribonuklease I-Varianten
DE602005019174D1 (de) * 2004-06-17 2010-03-18 Epigenomics Ag Zusammensetzungen und verfahren zur verhinderung des mitschleppens einer kontamination in nukleinsäure-amplifikationsreaktionen
WO2006034009A2 (en) * 2004-09-20 2006-03-30 Sigma-Aldrich Co. Purification of biomolecules from contaminating intact nucleic acids
JP4857260B2 (ja) 2005-03-08 2012-01-18 タカラバイオ株式会社 低温性微生物由来エンドヌクレアーゼ
US8309303B2 (en) 2005-04-01 2012-11-13 Qiagen Gmbh Reverse transcription and amplification of RNA with simultaneous degradation of DNA
EP1762627A1 (de) 2005-09-09 2007-03-14 Qiagen GmbH Verfahren zur Aktivierung einer Nukleinsäure für eine Polymerase-Reaktion
EP3211086B1 (de) * 2005-09-20 2021-06-09 Menarini Silicon Biosystems S.p.A. Verfahren und zusammensetzung zur erzeugung von einzelsequenz-dna-sonden, markierung von dna-sonden und verwendung dieser sonden
US9134237B2 (en) * 2005-09-20 2015-09-15 Janssen Diagnotics, LLC High sensitivity multiparameter method for rare event analysis in a biological sample
KR100804670B1 (ko) 2006-08-18 2008-02-20 명지대학교 산학협력단 정밀 중합효소 연쇄반응 방법
FR2914317B1 (fr) * 2007-03-30 2012-12-28 Biomerieux Sa Methode de decontamination d'une solution des acides nucleiques indesirables
EP2071034A1 (de) 2007-12-12 2009-06-17 bioMérieux Verfahren zur Behandlung einer Lösung zum Vernichten von Ribonukleinsäure nach der Amplifikation
US20090258365A1 (en) * 2008-03-25 2009-10-15 Terstappen Leon W M M METHOD FOR DETECTING IGF1R/Chr 15 in CIRCULATING TUMOR CELLS USING FISH
EP2294217A4 (de) * 2008-05-23 2012-10-31 Life Technologies Corp Verfahren zur abtrennung von nukleinsäurekontamination von reagentien
CA2730761C (en) * 2008-08-01 2016-04-19 F. Hoffmann-La Roche Ag Improved lysis and reverse transcription for mrna quantification
JP5517284B2 (ja) 2008-09-03 2014-06-11 西川ゴム工業株式会社 耐熱性２本鎖特異的核酸分解酵素
FR2940805B1 (fr) 2009-01-05 2015-10-16 Biomerieux Sa Procede d'amplification et/ou de detection d'acides nucleiques, kits et utilisations de ce procede
CA2749693C (en) 2009-01-15 2018-11-06 Hokkaido Mitsui Chemicals Inc. Enzyme preparation containing thermostable dna polymerase, method for producing same, and method for detecting subject organism to be detected
CA2760439A1 (en) 2009-04-30 2010-11-04 Good Start Genetics, Inc. Methods and compositions for evaluating genetic markers
US12129514B2 (en) 2009-04-30 2024-10-29 Molecular Loop Biosolutions, Llc Methods and compositions for evaluating genetic markers
GB2474225A (en) 2009-07-21 2011-04-13 Biotec Pharmacon Asa DNase for decontamination of reverse transcription and amplification reactions
ES2576927T3 (es) 2010-10-22 2016-07-12 T2 Biosystems, Inc. Sistemas de RMN y métodos para la detección rápida de analitos
US8563298B2 (en) 2010-10-22 2013-10-22 T2 Biosystems, Inc. NMR systems and methods for the rapid detection of analytes
US9163281B2 (en) 2010-12-23 2015-10-20 Good Start Genetics, Inc. Methods for maintaining the integrity and identification of a nucleic acid template in a multiplex sequencing reaction
DK2814954T3 (en) 2012-02-17 2017-05-15 Biotec Pharmacon Asa endonucleases
US8209130B1 (en) 2012-04-04 2012-06-26 Good Start Genetics, Inc. Sequence assembly
US10227635B2 (en) 2012-04-16 2019-03-12 Molecular Loop Biosolutions, Llc Capture reactions
US10851414B2 (en) 2013-10-18 2020-12-01 Good Start Genetics, Inc. Methods for determining carrier status
WO2016040446A1 (en) * 2014-09-10 2016-03-17 Good Start Genetics, Inc. Methods for selectively suppressing non-target sequences
CN105737395A (zh) * 2014-10-13 2016-07-06 崔荀 一种太阳能热水器加辅助电热水箱组合系统
EP3271480B8 (de) 2015-01-06 2022-09-28 Molecular Loop Biosciences, Inc. Abtastung auf strukturelle varianten
WO2017059132A1 (en) 2015-09-29 2017-04-06 The General Hospital Corporation Methods of treating and diagnosing disease using biomarkers for bcg therapy
CN111655258A (zh) * 2017-12-31 2020-09-11 常青树生物科学公司 用于冷冻保存的组合物和其使用方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0675966B1 (de) * 1992-02-19 2004-10-06 The Public Health Research Institute Of The City Of New York, Inc. Neue anordnungen von oligonukleotiden und ihr nutzen zum sortieren, isolieren, sequenzierung und manipulieren von nukleinsäuren
DE4314271A1 (de) * 1993-04-30 1994-11-03 Maiwald Matthias Dr Med Verfahren zur Elimination bakterieller DNA aus hitzestabiler DNA-Polymerase mittels eines DNA verdauenden Enzyms
CA2137728C (en) * 1993-12-14 1998-07-14 William E. Keating Enzymatic removal of non-target nucleic acid from biological samples
DE19513728A1 (de) * 1995-04-11 1996-10-31 Boehringer Mannheim Gmbh Verfahren zur Reduzierung der Bildung von Artefakten

Also Published As

Publication number Publication date
EP1003913B1 (de) 2007-12-12
US6541204B2 (en) 2003-04-01
WO1999007887A3 (en) 1999-04-29
AU749882B2 (en) 2002-07-04
JP2001512696A (ja) 2001-08-28
GB9716664D0 (en) 1997-10-15
EP1003913A2 (de) 2000-05-31
DE69838839T2 (de) 2008-12-04
JP3801865B2 (ja) 2006-07-26
US20020042052A1 (en) 2002-04-11
WO1999007887A2 (en) 1999-02-18
DE69838839D1 (de) 2008-01-24
AU8640298A (en) 1999-03-01

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