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ATE265542T1 - METHOD FOR HIGH-RESOLUTION IDENTIFICATION OF SOLVENT-ACCESSABLE AMIDE HYDROGENS IN POLYPEPTIDES - Google Patents

METHOD FOR HIGH-RESOLUTION IDENTIFICATION OF SOLVENT-ACCESSABLE AMIDE HYDROGENS IN POLYPEPTIDES

Info

Publication number
ATE265542T1
ATE265542T1 AT98940979T AT98940979T ATE265542T1 AT E265542 T1 ATE265542 T1 AT E265542T1 AT 98940979 T AT98940979 T AT 98940979T AT 98940979 T AT98940979 T AT 98940979T AT E265542 T1 ATE265542 T1 AT E265542T1
Authority
AT
Austria
Prior art keywords
solvent
protein
accessable
polypeptides
polypeptide
Prior art date
Application number
AT98940979T
Other languages
German (de)
Inventor
Virgil L Woods Jr
Original Assignee
Exsar Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Exsar Corp filed Critical Exsar Corp
Application granted granted Critical
Publication of ATE265542T1 publication Critical patent/ATE265542T1/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6878—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids in epitope analysis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N2030/022—Column chromatography characterised by the kind of separation mechanism
    • G01N2030/027—Liquid chromatography
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
    • G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/795—Porphyrin- or corrin-ring-containing peptides
    • G01N2333/805—Haemoglobins; Myoglobins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/95—Proteinases, i.e. endopeptidases (3.4.21-3.4.99)
    • G01N2333/964—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue
    • G01N2333/96425—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals
    • G01N2333/96427—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general
    • G01N2333/9643—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general with EC number
    • G01N2333/96472—Aspartic endopeptidases (3.4.23)
    • G01N2333/96475—Aspartic endopeptidases (3.4.23) with definite EC number
    • G01N2333/96477—Pepsin (3.4.23.1; 3.4.23.2; 3.4.23.3)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/24—Nuclear magnetic resonance, electron spin resonance or other spin effects or mass spectrometry
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/25125—Digestion or removing interfering materials

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)

Abstract

The present invention provides methods whereby the positions of peptide amide groups that are labeled with a heavy hydrogen in a polypeptide or protein can be localized at high resolution. The methods are useful for determining which peptide amide groups in a polypeptide or protein are accessible to solvent, mapping the binding site and/or binding surface of a binding protein, and/or studying allosteric or other conformational changes in a polypeptide or protein which alter the rates at which certain peptide amide hydrogens exchange with solvent.
AT98940979T 1997-08-19 1998-08-18 METHOD FOR HIGH-RESOLUTION IDENTIFICATION OF SOLVENT-ACCESSABLE AMIDE HYDROGENS IN POLYPEPTIDES ATE265542T1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/919,187 US6291189B1 (en) 1994-05-10 1997-08-19 Methods for the high-resolution identification of solvent-accessible amide hydrogens in polypeptides or proteins and for characterization of the fine structure of protein binding sites
PCT/US1998/017135 WO1999009204A1 (en) 1997-08-19 1998-08-18 Methods for the high-resolution identification of solvent-accessible amide hydrogens in polypeptides

Publications (1)

Publication Number Publication Date
ATE265542T1 true ATE265542T1 (en) 2004-05-15

Family

ID=25441669

Family Applications (1)

Application Number Title Priority Date Filing Date
AT98940979T ATE265542T1 (en) 1997-08-19 1998-08-18 METHOD FOR HIGH-RESOLUTION IDENTIFICATION OF SOLVENT-ACCESSABLE AMIDE HYDROGENS IN POLYPEPTIDES

Country Status (8)

Country Link
US (2) US6291189B1 (en)
EP (1) EP1012325B1 (en)
AT (1) ATE265542T1 (en)
AU (1) AU8913498A (en)
CA (1) CA2300100A1 (en)
DE (1) DE69823525T2 (en)
IL (1) IL134565A0 (en)
WO (1) WO1999009204A1 (en)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6599707B1 (en) * 1998-09-11 2003-07-29 Exsar Corporation Methods for identifying hot-spot residues of binding proteins and small compounds that bind to the same
CA2407703A1 (en) * 2000-04-28 2001-11-08 Duke University A quantitative, high-throughput screening method for protein stability
US6734023B1 (en) 2000-04-28 2004-05-11 Duke University Quantitative, high-throughput screening method for protein stability
KR20030074773A (en) * 2001-02-01 2003-09-19 싸이퍼젠 바이오시스템즈, 인코포레이티드 Improved methods for protein identification, characterization and sequencing by tandem mass spectrometry
WO2003034177A2 (en) * 2001-10-04 2003-04-24 California Institute Of Technology Method for determining molecular surface areas and volumes
WO2003087133A2 (en) * 2002-04-10 2003-10-23 The Regents Of The University Of California Improved methods for high-resolution identification of solvent-accessible amide hydrogens in polypeptides and for characterization of polypeptide structure
AU2003277423A1 (en) * 2002-10-18 2004-05-04 The Regents Of The University Of California Methods for crystallographic structure determination employing hydrogen exchange analysis
US7368290B2 (en) * 2003-05-12 2008-05-06 Sandia National Laboratories Structural determination of intact proteins using mass spectrometry
US20070122864A1 (en) * 2003-11-05 2007-05-31 The Regents Of The University Of California Methods for the determination of protein three-dimensional structure employing hydrogen exchange analysis to refine computational structure prediction
US7363171B2 (en) * 2003-11-26 2008-04-22 The Regents Of The University Of California Enhanced methods for crystallographic structure determination employing hydrogen exchange analysis
US20070087448A1 (en) * 2004-02-16 2007-04-19 Nelsestuen Gary L Biological profiles and methods of use
EP1765078A2 (en) * 2004-07-02 2007-03-28 Exsar Corporation Drug candidate selection by hydrogen exchange characterization of ligand-induced receptor conformation
WO2006130474A2 (en) 2005-05-27 2006-12-07 Ionwerks, Inc. Multi-beam ion mobility time-of-flight mass spectrometer with bipolar ion extraction and zwitterion detection
US7983887B2 (en) 2007-04-27 2011-07-19 Ut-Battelle, Llc Fast computational methods for predicting protein structure from primary amino acid sequence
WO2009048460A1 (en) * 2007-10-09 2009-04-16 Locus Pharmaceuticals, Inc. Methods and systems for grand canonical competitive simulation of molecular fragments
US20090094012A1 (en) * 2007-10-09 2009-04-09 Locus Pharmaceuticals, Inc. Methods and systems for grand canonical competitive simulation of molecular fragments
US9140728B2 (en) * 2007-11-02 2015-09-22 Emerson Climate Technologies, Inc. Compressor sensor module
EP2419918A4 (en) * 2009-04-14 2016-12-21 Univ Northeastern RAPID GAS PHASE ISOTOPIC MARKING FOR ENHANCED DETECTION OF PROTEIN CONFORMS
JP5972871B2 (en) 2010-07-20 2016-08-17 テバ・ファーマシューティカルズ・オーストラリア・ピーティワイ・リミテッド Anti-IL-23 heterodimer specific antibody
EP2603522A1 (en) 2010-08-13 2013-06-19 GlaxoSmithKline Intellectual Property Development Limited Improved anti-serum albumin binding variants
EP2852615B1 (en) 2012-05-22 2018-12-05 Bristol-Myers Squibb Company Il-17a/f il-23 bispecific antibodies and their uses
US10126304B2 (en) 2013-01-16 2018-11-13 George Mason Research Foundation, Inc. Binding domain mapping
EP3585892B8 (en) * 2017-02-27 2022-07-13 Translate Bio, Inc. Methods for purification of messenger rna

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US3560158A (en) 1965-08-12 1971-02-02 Monsanto Co Method for analysis of labile hydrogen containing compounds
US3623840A (en) 1969-01-02 1971-11-30 Monsanto Co Analytical method and apparatus for analysis of labile hydrogen containing compounds
FR2154989A5 (en) 1971-10-01 1973-05-18 Commissariat Energie Atomique
US4153416A (en) 1977-06-06 1979-05-08 Bonner Alex G Process and apparatus for pulse labelling protein material in the Edman degradation process
US4661111A (en) 1982-08-04 1987-04-28 La Jolla Cancer Research Foundation Polypeptide
US4578079A (en) 1982-08-04 1986-03-25 La Jolla Cancer Research Foundation Tetrapeptide
US4614517A (en) 1982-08-04 1986-09-30 La Jolla Cancer Research Foundation Tetrapeptide
US4792525A (en) 1982-08-04 1988-12-20 La Jolla Cancer Research Foundation Tetrapeptide
US4589881A (en) 1982-08-04 1986-05-20 La Jolla Cancer Research Foundation Polypeptide
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US5030565A (en) 1983-08-17 1991-07-09 Scripps Clinic And Research Foundation Polypeptide-induced monoclonal receptors to protein ligands
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US5658739A (en) * 1994-05-10 1997-08-19 The Regents Of The University Of California Method for characterization of the fine structure of protein binding sites

Also Published As

Publication number Publication date
EP1012325A4 (en) 2001-10-04
AU8913498A (en) 1999-03-08
EP1012325B1 (en) 2004-04-28
WO1999009204A1 (en) 1999-02-25
US6797482B2 (en) 2004-09-28
DE69823525T2 (en) 2005-04-07
IL134565A0 (en) 2001-04-30
EP1012325A1 (en) 2000-06-28
DE69823525D1 (en) 2004-06-03
CA2300100A1 (en) 1999-02-25
US20020042080A1 (en) 2002-04-11
US6291189B1 (en) 2001-09-18

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