OA16292A

OA16292A - Methods for the preparation of diasteromerically pure phosphoramidate prodrugs.

Info

Publication number: OA16292A
Application number: OA1201200546
Authority: OA
Inventors: Michael O'neil Hanrahan Clarke; Aesop Cho; Scott Alan Wolckenhauer
Original assignee: Gilead Sciences, Inc.
Priority date: 2010-07-19
Filing date: 2011-07-19
Publication date: 2015-04-24

Abstract

Provided are methods and intermediates for preparing diastereomerically pure phosphoramidate prodrugs of nucleosides of Formulas (la) and (lb) :

Description

Gilead Sciences _t Inc·

METHODS FOR THE PREPARATION OF DIASTEROMERICALLY PURE PHOSPHORAMIDATE PRODRUGS

FIELD OF THE INVENTION

The invention relates generally to methods for preparing compounds with antiviral activity, most particularly to prodrugs of inhibitors of hepatitis C virus RNAdependent RNA polymerase.

BACKGROUND OF THE INVENTION

The hepatitis C virus (HCV) is a leading cause of chronic liver disease worldwide (Boyer, N. et al. J Hepatol. 32:98-112, 2000) and may lead to hepatic fibrosis, cirrhosis and hepatocellular carcinoma (Cale, P., Gastroenterolgy Clin. Biol. 2009, 33, 958). A significant focus of current antiviral research is directed toward the development of improved methods of treatment of chronic HCV infections in humans (Di Besceglie, A.M. and Bacon, B. R., Scientific American, Oct.: 80-85, (1999); Gordon, C. P., et al., J. Med. Chem. 2005, 48, 1-20; Maradpour, D.; et al., Nat. Rev. Micro. 2007,5(6), 453-463). A number of HCV treatments are reviewed by Bymock et al. in Antiviral Chemistry & Chemotherapy, 11:2; 79-95 (2000).

Currently, there are primarily two antiviral compounds, ribavirin, a nucleoside analog, and interferon-alpha (a) (IFN), which are used for the treatment of chronic HCV infections in humans. Ribavirin alone is not effective in reducing viral RNA levels, has significant toxicity, and is known to induce anemia. The combination of IFN and ribavirin has been reported to be effective in the management of chronic hepatitis C (Scott, L. J., et al. Drugs 2002, 62, 507-556) but less than half the patients given this treatment show a persistent benefit. Other patent applications disclosing the use of nucleoside analogs to treat hepatitis C virus include WO 01/32153, WO 01/60315, WO 02/057425, WO 02/057287, WO 02/032920, WO 02/18404, WO 04/046331, W02008/089105 and W02008/141079 but additional treatments for HCV infections have not yet become available for patients. Therefore, drugs having improved antiviral and pharmacokinetic properties with enhanced activity against development of HCV resistance, improved oral bioavailability, greater efficacy, fewer undesirable side effects and extended effective half-life in vivo (De Francesco, R. et al. (2003) Antiviral Research 58:1-16) are urgently needed.

RNA-dependent RNA polymerase (RdRp) is one of the best studied targets for the development of novel HCV therapeutic agents. The NS5B polymerase is a target for inhibitors in early human clinical trials (Sommadossi, J., WO 01/90121 A2, US 2004/0006002 Al). These enzymes have been extensively characterized at the biochemical and structural level, with screening assays for identifying selective inhibitors (DeClercq, E. (2001) J. Pharmacol. Exp.Ther. 297:1-10; De Clercq, E. (2001) J. Clin. Virol. 22:73-89). Biochemical targets such as NS5B are important in developing HCV therapies since HCV does not replicate in the laboratory and there are difficulties in developing cell-based assays and preclinical animal systems.

Inhibition of viral replication by nucleosides has been extensively studied (De

Clercq, E. ¢2001) J. Clin. Virol. 22:73-89) including nucleosides that inhibit RdRp. Generally, the antiviral activity of these nucleosides are attributed to the conversion of the nucleosides to their nucleoside triphosphates (NTPs) which act as inhibitors of DNA and RNA polymerases or as chain terminators following incorporation into the lengthening viral DNA or RNA strand. However, many NTPs lack adequate specificity for viral polymerases compared to host polymerases and, as a result, cause substantial toxicity. This has led to efforts to modify the core structures of nucleosides to achieve higher selectivity but many of the structural modifcations have simultaneously compromised NTP production in the cells (Yamanaka, Antimicrob.

Agents Chemother. 1999: 190-193).

The poor conversion of the nucleoside to NTP can often be attributed to the inability of nucleoside kinases to convert the nucleoside to the nucleoside 5’monophosphate (NMP). NMP prodrugs have been used to bypass poor nucleoside kinase activity (Schultz, Bîoorg. Med. Chem. 2003, 11, 885). Among these prodrugs, 30 NMP phosphoramidates have been reported to increase intracellular concentrations of NTP compared to the nucleoside alone (McGuigan, J. Med. Chem. 1993, 36, 1048

1052). However, these NMP prodrugs are substrates for esterases and phosphodiesterases in the blood and other body tissues which can cleave the prodrug to a charged molelcule or to the nucleoside, respectively. The charged molecule is then impermeable to the target organ or cell and the nucleoside is poorly phosphorylated intracellularly.

The development of a highly effective, non-toxic NMP prodrug is largely an unpredictable trial and error exercise requiring the balancing of the stability of the NMP prodrug in blood with the ability of the prodrug to reach a target organ or cell, be absorbed or actively taken up by the target cell, being efficiently cleaved to the NMP intracellularly and subsequently converted to a NTP that is selective for inhibiting the viral polymerase (Perrone, J. Med. Chem. 2007, 50, 1840-49; Gardelli, J. Med. Chem. 2009, 52, 5394-5407). For the case of an orally effective RdRp inhibitor for treating HCV infection, the NMP prodrug would need to be chemically stable to the conditions of the upper intestinal tract, be efficiently absorbed from the intestinal tract, survive the many esterases of the intestinal cells and blood, be efficiently extracted by the hepatocytes, and be cleaved to the NMP and subsequently converted to a NTP in hepatocytes that is specific for inhibiting the HCV NS5B polymerase. Notably, the anti-HCV activity of phosphate prodrugs can markedly depend upon the chirality of the phosphorous in the prodrug (Gardelli, J. Med. Chem. 2009, 52, 5394-5407; Meppen, Abstracts of Papers, 236th ACS National Meeting, Philadelphia, PA, United States, August 17-21, 2008 (2008), MEDI-404.).

Babu, Y. S., W02008/089105 and W02008/141079, discloses ribosides of pyrrolo[l,2-f][l,2,4]triazine nucleobases with antiviral, anti-HCV, and anti-RdRp activity.

Butler, et al., WO2009132135, disclose 1’ substituted ribosides and prodrugs comprising pyrrolo[l,2-f][l,2,4]triazine nucleobases which have anti-HCV and antiRdRp activity but does not disclose species of the 3’-O-acylated derivatives of those ribosides or the expected properties of such derivatives. Cho, et al., US 61353351, discloses 3’-O-acylated 1 ’substituted ribosides phosphate prodrugs comprising pyrrolof 1,2-f][ 1,2,4]triazine nucleobases that have anti-HCV activity that are efficiently delivered to the liver after oral administration. The efficient delivery of the prodrugs to the liver is dependent on the chirality of the phosphorous prodrug.

In view of the importance of anti-HCV therapeutics that are NMP prodrugs with chiral phosphorous atoms such as those described by Cho, et al., Gardelli, et al., Perrone et al., and Meppen, et al., new efficient methods of producing chiral phosphates of these prodrugs are needed.

SUMMARY OF THE INVENTION

Provided are methods for preparing compounds that inhibit hepatitis C virus. The compounds are prodrugs of nucleoside monophosphates that, when administered to animals, are intracellularly converted to nucleoside triphosphates. The chirality of the phosphorous atom determines the efficiency of the conversion to the nucleoside triphosphate in the animal. The method disclosed, provides a convergent synthesis of these single diastereomeric prodrugs which is an improvement over the previously disclosed chromatographic methods of separating a single diastereomer from a mixture of diastereomers.

In one embodiment, provided is a method for preparing a compound of Formula la or lb:

R²² R²

Formula Ib or a pharmaceutically acceptable salt or acid thereof;

wherein:

each R¹, R², R⁷, R²², R²³ or R²⁴ is independently H, OR¹¹, NRⁿR¹², C(O)NR^hR^!2, -OC(O)NR^llR¹², C(O)OR^t], OC(O)0R^!1, S(O)nR“ S(O)2NRR¹², N3, CN, halogen, (Ci-C₈)alkyl, (C₃-C₈)carbocyclyl, (C₄-C₈)carbocyclylalkyl, (C₇ -CxJalkenyL (C₂-C₈)alkynyl or aryl(C_rC₈)alkyl;

or any two R¹, R², R⁷, R²², R²³ or R²⁴ on adjacent carbon atoms when taken together are -O(CO)O- or -O(CR' ^lR¹²)O- or when taken together with the ring carbon atoms to which they are attached form a double bond;

each Base is independently a naturally occurring or modified purine or pyrimidine base linked to the furanose ring through a carbon or nitrogen atom;

each n is independently 0, 1, or 2;

each R^a, R⁴ or R⁶ is independently (Ci-C₈)alkyl, (C₂-C₈)alkenyl, (C₂C₈)alkynyl, (C₃-C₈)carbocyclyl, (C₄-C₈)carbocyclyl alkyl, aryl(Ci-C₈)alkyl, heterocyclyl(C_t-C₈)alkyl, (C₆-C₂o)aryl, (C₂-C₂o)heterocyclyl or heteroaryl;

each R^c or R^d is independently H, (C_t-C₈)alkyl, (C₂-C₈)alkenyl, (C₂C₈)alkynyl, (C₃-C₈)carbocyclyl, (C₄-C₈)carbocyclyl alkyl, aryl(Ci-C₈)alkyl, heterocyclyl(Ci-C₈)alkyl, (C₆-C₂o)aryl, (C₂-C₂o)heterocyclyl or heteroaryl provided that R^c and R^d are not the same;

each R⁵ is independently H, (C₍-C₈)alkyl, (C₂-C₈)alkenyl, (C₂-C₈)alkynyl, (C₃C₈)carbocyclyl, (C₄-C₈)carbocyclylalkyl, aryl(C]-C₈)alkyl, heterocyclyl(Cj-C₈)alkyl, (C₆-C₂o)aryl, (C₂-C₂₀)heterocyclyl or heteroaryl;

each R or R ² is independently H? (CpCg)alkyl, (C2-Cg)alkenyl, (C2C8)a[kynyl. (CvQ)carbocyclyl, (C4-Cg)carbocyclylalkyl, aryl(C]-Cg)alkyl, heterocyclyl(Ci-Cg)alkyl, (C6-C2o)aryl, (CL-C2o)heterocyclyl, heteroaryl, -C(=O)(CiCg)alkyl, -S(O)n(Ci-Cg)aIkyl or R¹¹ and R¹² taken together with a nitrogen to which they are both attached form a 3 to 7 membered heterocyclic ring wherein any one carbon atom of said heterocyclic ring can optionally be replaced with -0-, -S(O)n- or -NR¹'-; and wherein each (Ci-Cg)alkyl, (C₂-Cg)alkenyl, (C₂-Cg)alkynyl, (C₃Cg)carbocyclyl, (C4-Cg)carbocyclylalkyl, aryl(Ci-Cg)alkyl, heterocyclyl(Ci-Cg)alkyl, (C6-C₂o)aryl, (C₂-C₂o)heterocyclyl or heteroaryl of each R^c, R^d, R¹, R², R²², R²³, R²⁴, R⁴, R⁵, R⁶, R⁷, R¹¹ or R¹² is, independently, optionally substituted with one or more halo, hydroxy, CN, N3, N(R^a)2, NH(R^a), NH2, NO2, C(0)N(R^a)2, C(0)NH(R^a), C(0)NH2, 0C(0)N(R^a)2, OC(O)NH(R^a), OC(O)NH2, C(0)0R^a, OC(O)OR^a, C(0)R^a, OC(0)R^a, S(O)nR^a S(O)2N(R^a)2, S(O)2NH(R^a), S(O)2NH₂, OR^a or R^a;

said method comprising:

(a) providing a compound of Formula II

Formula II and (b) treating the compound of Formula II with a compound of Formula Illa and abase

thereby forming a compound of Formula la or

(c) treating the compound of Formula II with a compound of Formula Illb and a base

thereby forming a compound of Formula lb;

wherein:

each Ar is a O-C» aryl or heteroaryl wherein said (C0--C20) aryl or heteroaryl is substituted with one or more halogen, NO₂, or (Cj-Cgjhaloalkyl and optionally substituted with one or more CN, N₃, N(R^a)2, C(O)N(R^a)2, OC(O)N(R^a)2, C(O)OR^a, 0C(O)OR^a, C(O)R^a, OC(O)R^a, S(O)nR^a S(O)2N(R^a)₂, OR^a or R^a with the proviso that Ar is different from R⁴. In another aspect, Base is not uracil. In another aspect, Base is not cytosine.

In another aspect, the invention also provides novel intermediates disclosed herein which are useful for preparing Formula la or Formula lb.

In other aspects, methods for the synthesis, analysis, separation, isolation, purification, and characterization of the novel intermediates of this invention are provided.

DETAILED DESCRIPTION OF EXEMPLARY EMBODIMENTS

Reference will now be made in detail to certain embodiments of the invention, examples of which are illustrated in the accompanying description, structures and formulas. While the invention will be described in conjunction with the enumerated embodiments, it will be understood that they are not intended to limit the invention to those embodiments. On the contrary, the invention is intended to cover all alternatives, modifications, and equivalents, which may be included within the scope of the present invention.

Typically, the method of for preparing a compound of Formula la from Formula II and Formula Illa or Formula lb from Formula II and Formula IHb is performed in a suitable solvent. The suitable solvent is preferably an anhydrous, nonacid, non-hydroxylic solvent. Non-limiting examples of suitable solvents are ethers, for example, diethyl ether, diisopropyl ether, di /-butyl ether, tetrahydrofuran, dioxane and various glyme solvents; dimethylformamide or dimethyiacetamide. A preferred solvent is tetrahydrofuran. The concentration of Formula II in the solvent is typically about 0.01 to about 1 mole per liter of solvent. The method is performed at a temperature of about 0 °C to about 80 °C, more preferably about 20 °C to about 60 °C.

The solution of Formula II is typically treated with a hindered base or a nonnucleophilic base. Typical, but non-limiting, examples of hindered bases are tbutyllithium, sec-isobutyllithium, lithium or sodium diisopropylamide and tbutylmagnesium halides. A preferred hindered base is rbutylmagnesium chloride. Typical, but non-limiting, examples of non-nucleophic bases are sodium hydride, potassium hydride, lithium hydride and calcium hydride. The hindered bases or nonnucleophic bases may be used as solutions in or as undiluted bases. Preferably, the bases are used as solutions in anhydrous, non-hydroxylic solvents wherein the concentration of the base in the solvent is about 0.5 to about 3 moles per liter. The molar ratio of base to the compound of Formula II is about 1:1 to about 3:1, preferably about 1.1:1 to about 1.5:1. The solution of the compound of Formula II is typically treated with the base for about 5 minutes to about two hours, preferably less than 30 minutes.

The mixture of the solution of the compound of Formula II and the base is treated with a compound of Formula Illa or Formula IHb for about 30 minutes to about 24 hours, preferably about one to about four hours. The molar ratio of the compound Formula II to the compound of Formula Illa or Formula IHb is typically about 1:1 to about 1:4. Preferably, the molar ratio is about 1:1.1 to about 1:2.

In another embodiment of the method for preparing a compound of Formula la or lb or a pharmaceutically salt or ester thereof, Formula la is Formula IVa, Formula lb is Formula IVb and Formula II is Formula V:

R²² R²

Formula V

In one embodiment of the method for preparing a compound of Formula IVa or IVb from a compound of Formula V, R? is H, halogen, optionally substituted (Ci-Cg)alkyl, optionally substituted (C₂-Cg)alkenyl or optionally substituted (C₂-Cg)alkynyl. In another aspect of this embodiment, R¹ is H. In another aspect of this embodiment, R¹ is optionally substituted (C_t-Cg)alkyl. In another aspect of this embodiment, R¹ is methyl. In another aspect of this embodiment, R^l is optionally substituted (C/ C/Jalkenyl. In another aspect of this embodiment, R¹ is optionally substituted (C2-Cg)alkynyl. In another aspect of this embodiment, R¹ is F. In another aspect of this embodiment, R is F. In another aspect of this embodiment, R is OR . In another aspect of this embodiment, R² is OH. In another aspect of this embodiment, R⁷ is H. In another aspect of this embodiment, R⁷ is optionally substituted (C,—C8)alkyl. In another aspect of this embodiment, R⁷ is optionally substituted (C2-C8)alkynyl. In another aspect of this embodiment, R⁷ is CN. In another aspect of this embodiment, R^s is H. In another aspect of this embodiment, one of R^c or R^d is H. In another aspect of this embodiment, one of R^c or R^d is H and the other of R^c or R^d is optionally substituted (Ci-C8)alkyl. In another aspect of this embodiment, R⁶ is optionally substituted (Cj-CgJalkyl. In another aspect of this embodiment, R⁴ is optionally substituted (C6-C2o)aryl. In another aspect of this embodiment, Ar is optionally substituted nitrophenyl. In another aspect of this embodiment, R²³ is H. In another aspect of this embodiment, R²² is OR¹¹. In another aspect of this embodiment, R is OH. In another aspect of this embodiment, R is N₃. In another aspect of this embodiment, R²⁴ is H. In another aspect of this embodiment, Base is selected from the group consisting of:

wherein:

each X¹ is independently N or CR¹⁰;

each X is independently NR , O, or S(O)_n;

each R⁸ is independently halogen, NR^liR^l2, NiR'^OR¹¹, NR^hNR^uR¹², N3, NO, NO2, CHO, CN, -CH(=NRⁿ), -CH=NNHR^U, -CH=N(OR^h), -CH(ORⁿ)2, -C(=O)NR^UR¹², -C(=S)NRⁿR¹², -C(=O)ORⁿ, (C_rC_s)alkyl, (C₂-C₈)alkenyl, (C₂C₈)alkynyl, (C₄-C₈)carbocyclylalkyl, (C₆-C₂₀)aryl, (C₂-C₂o)heterocyclyl, heteroaryl, -C(=O)(Ci-C₈)alkyl, -S(O)_n(C_rC₈)alkyl, aryl(C_rC_g)alkyl, OR¹¹ or SR¹¹;

each n is independently 0,1, or 2;

each R⁹ or R¹⁰ is independently H, halogen, NR^1!R¹², NtR’^OR¹¹, NR^1]NR^ltR¹², N3, NO, NO2, CHO, CN, -CH(=NR¹¹), -CH=NHNR^1!, -CH=N(OR^1!), -CH(OR^h)2, -C(=O)NRⁿR¹²,-C(=S)NR^HR¹², -C(=O)OR^I!, R^h, 0R^!i or SR^!1;

each R^!1 or R^1Z is independently H, (CrC8)alkyl, (C2-C8)alkenyl, (C2C8)alkynyl, (C3-C8)carbocyclyl, (C4-C8)carbocyclylalkyl, aryl(Ci-C8)aIkyl, heterocyclyl(Ci-C8)alkyl, (C6-C2o)aryl, (C2-C20)heterocyclyl, heteroaryl, -C(=O)(C]C8)alkyl, -S(O)n(Ci-C8)alkyl or R¹¹ and R¹² taken together with a nitrogen to which they are both attached form a 3 to 7 membered heterocyclic ring wherein any one carbon atom of said heterocyclic ring can optionally be replaced with -O-, -S(O)n- or -NR^a-; and wherein each (Ci-C₈)alkyl, (C₂-C₈)alkenyl, (C₂-C₈)alkynyl, (C₃C₈)carbocyclyl, (C₄-C₈)carbocyclylalkyl, aryl(Cj-C₈)alkyl, heterocyclyl(Ci-C₈)alkyl, (C6-C₂o)aryl, (C₂-C₂o)heterocyclyl or heteroaryl of each R^c, R^d, R¹, R², R²², R²³, R²⁴, R⁴, R⁵, R⁶, R⁷, R⁸, R⁹, R¹⁰, Rⁿ or R¹² is, independently, optionally substituted with one or more halo, hydroxy, CN, N3, N(R^a)2, NH(R^a), NH2, NO2, C(O)N(R^a)2, C(O)NH(R^a), C(O)NH2, OC(O)N(R^a)2, OC(O)NH(R^a), OC(O)NH2, C(O)OR^a, OC(O)OR^a, C(O)R^a, OC(O)R^a, S(O)nR^a S(O)₂N(R^a)2, S(O)2NH(R^a), S(O)2NH₂, OR^aor R^a. In another aspect, Base is not uracil. In another aspect, the Base is not cytosine.

In another aspect of this embodiment, Base is selected from the group consisting of:

In another aspect of this embodiment, Base is selected from the group

In another embodiment of the method for preparing a compound of Formula IVa or IVb from a compound of Formula V, R¹ is H, halogen, optionally substituted (Ci - Cs)alkyl, optionally substituted (C₂-C₈)alkenyl or optionally substituted (C₂-C₈)alkynyl; R² is OR¹¹ or halogen; R²² is OR¹¹ and each R^s, R²³ and R²⁴ is H. In another aspect of this embodiment, R¹ is H. In another aspect of this embodiment, R¹ is optionally substituted (Ci-Cg)alkyl. In another aspect of this embodiment, R^! is methyl. In another aspect of this embodiment, R¹ is optionally substituted (C2-C8)alkenyl. In another aspect of this embodiment, R¹ is optionally substituted (C2-C$)a!kynyl. In another aspect of this embodiment, R¹ is F. In another aspect of this embodiment, R is F. In another aspect of this embodiment, R is OH. In another aspect of this embodiment, R²² is OH. In another aspect of this embodiment, each R² and R²² is OH. In another aspect of this embodiment, R⁷ is H. In another aspect of this embodiment, R⁷ is optionally substituted (Ci-Cg)alkyl. In another aspect of this embodiment, R⁷ is optionally substituted (C2-C8)alkynyl. In another aspect of this embodiment, R⁷ is CN. In another aspect of this embodiment, one of R^c or R^d is H. In another aspect of this embodiment, one of R^c or R^d is H and the other of R^c or R^d is optionally substituted (CrC₈)alkyl. In another aspect of this embodiment, R⁶ is optionally substituted (Ci-Cg)alkyl. In another aspect of this embodiment, R⁴ is optionally substituted (C6-C₂o)aryl. In another aspect of this embodiment, Ar is optionally substituted nitrophenyl. In another aspect of this embodiment, Base is selected from the group consisting of:

wherein:

each X¹ is independently N or CR¹⁰;

each X is independently NR , O, or S(O)_n;

each R⁸ is independently halogen, NR¹ ‘r¹², N(R* ‘jOR* *, NR¹ W 'R¹², N3, NO, N02, CHO, CN, -CH(=NRⁿ), -CH^NNHR¹¹, -CH^NtOR¹¹), -CH(0Rⁿ)2, -C(=O)NRⁿR¹², -C(=S)NR’‘R¹², -C(=0)OR^h, (C|-C₈)alkyl, (C₂-C₈)alkenyl, (C₂C₈)alkynyl, (C4-C₈)carbocyclylalkyl, (C6-C₂o)aryl, (C₂-C₂o)heterocyclyl, heteroaryl, -C(=O)(C]-C_g)aIkyI, -S(O)_n(Ci-C₈)alkyl, aryl(C_rC₈)alkyl, OR¹¹ or SR¹¹;

each n is independently 0, 1, or 2;

each R⁹ or R^î0 is independently H, halogen, NRⁿR¹², N(Rⁿ)OR¹¹, NRⁿNR^llR¹², N3, NO, NO2, CHO, CN, -CH(=NRⁿ), -CH=NHNR¹¹, -CH=N(ORⁿ), -CH(ORⁿ)2> -C(=O)NR¹¹R^!2,-C(=S)NR¹¹R¹²,-C(=0)OR^H,R¹¹,OR¹¹ or SR¹¹;

each R¹¹ or R¹² is independently H, (Ci-Cg)alkyl, (C2-C8)alkenyl, (C2Cg)alkynyl, (C3-Cg)carbocyclyl, (C4-C8)carbocyclylalkyl, aryl(Ci-C8)alkyl, heterocyclyl(Ci-C8)alkyl, (C<, -C2o)aryl, (C2-C2o)heterocyclyl, heteroaryl, -C(=O)(C]Cg)alkyl, -S(O)n(Ci-Cg)alkyl or R¹¹ and R¹² taken together with a nitrogen to which they are both attached form a 3 to 7 membered heterocyclic ring wherein any one carbon atom of said heterocyclic ring can optionally be replaced with -0-, -S(O)n- or -NR^a-; and wherein each (Ci-C₈)alkyl, (C₂-C₈)alkenyl, (C₂-Cg)alkynyl, (C₃C₈)carbocyclyl, (Cj-Cgjcarbocyclylalkyl, aryl(C]-C₈)alkyl, heterocyclyl(Ci-C₈)alkyl, (C6-C₂₀)aryl, (C₂-C₂o)heterocyclyl or heteroaryl of each R^c, R^d, R¹, R², R²², R²³, R²⁴, •λ/*

R⁴, R⁵, R⁶, R⁷, R⁸, R⁹, R¹⁰, R¹¹ or R¹² is, independently, optionally substituted with one or more halo, hydroxy, CN, Nj, NI(R^a)₂. NH(R^a), NH2, NO2, C(O)N(R^a)2, C(O)NH(R^a), C(O)NH₂, 0C(O)N(R^a)₂, OC(O)NH(R^a), OC(O)NH2, C(O)OR^a, OC(O)OR^a, C(O)R^a, OC(O)R^a, S(O)nR^a, S(O)₂N(R^a)2, S(O)2NH(R^a), S(O)2NH₂, OR^a or R^a. In another aspect, Base is not uracil. In another aspect, the Base is not cytosine.

In another aspect of this embodiment, Base is selected from the group

consisting of

In another embodiment of the method for preparing a compound of Formula

IVa or IVb from a compound of Formula V, R¹ is H or CH3; R² is OR¹¹ or halogen; R⁶ is optionally substituted (Ci-Cg)alkyl; one of R^c or R^d is H and the other of R^c or R^d is optionally substituted (Ci C8)alkyl; R²² is OR¹¹, and each R⁵, R²³ and R²⁴ is H. In another aspect of this embodiment, R¹ is H. In another aspect of this embodiment, R is CH₃, In another aspect of this embodiment, R is F. In another aspect of this embodiment, R is OH. In another aspect of this embodiment, R is OH. In another aspect of this embodiment, each R and R is OH. In another aspect of this embodiment, R⁷ is H. In another aspect of this embodiment, R⁷ is CH₃. In another aspect of this embodiment, R⁷ is ethynyl. In another aspect of this embodiment, R⁷ is CN. In another aspect of this embodiment, R⁴ is optionally substituted (Ce-Cïojaryl.

In another aspect of embodiment, R⁴ is optionally substituted phenyl. In another aspect of this embodiment, R⁴ is optionally substituted napthyl. In another aspect of this embodiment, Ar is optionally substituted nitrophenyl. In another aspect of this embodiment, Base is selected from the group consisting of:

wherein:

each X^! is independently N or CR^l°;

each X² is independently NR¹', O, or S(O)_n;

each R⁸ is independently halogen, NRⁿR¹², N(R^t!)OR, NR^!1NRⁿR¹², N3, NO, NO2, CHO, CN, -CH(=NR^H), -CH=NNHR¹¹, -CH=N(OR¹¹), -CH(OR^ll)2, -C(=O)NR^hR¹², -C(=S)NR^llR¹², -C(O)OR¹¹, (Cj-CgJalkyl, (C₂-C₈)alkenyl, (C₂C₈)alkynyl, (C4-C₈)carbocyclylalkyl, (C6-C₂o)aryl, (C₂-C₂o)heterocyclyl, heteroaryl, -C(=O)(Ci-C₈)alkyl, -SfOUCrCgjalkyl, aryl(C_rC₈)alkyI, OR¹¹ or SR¹¹;

each n is independently 0, 1, or 2;

each R⁹ or R¹⁰ is independently H, halogen, NRⁿR¹², NfR'^OR¹¹, NR¹¹NR^ltR¹², N3, NO, NO2, CHO, CN, -CH(=NR^li), -CH=NHNR¹¹, -CH=N(OR¹¹), -CH(OR^1!)2, -C(=O)NRⁿR¹², -C(=S)NR“R¹², -C(=O)ORⁿ, R¹¹, OR¹¹ or SR¹¹;

each R^!1 or R¹² is independently H, (Ci-C₈)alkyl, (C₂-C₈)alkenyl, (C₂C₈)alkynyl, (C3-C₈)carbocyclyl, (C₄-Cg)carbocyclylalkyl, aryl(Ci-C₈)alkyl, heterocyclyl(Ci-C₈)alkyl, (Ce-C₂o)aryl, (C2-C₂o)heterocyclyl, heteroaryl, -C(=O)(CiCg)alkyl, -S(O)_n(C[-C₈)alkyl or R¹¹ and R¹² taken together with a nitrogen to which they are both attached form a 3 to 7 membered heterocyclic ring wherein any one carbon atom of said heterocyclic ring can optionally be replaced with -O-, -S(O)_n- or -NR^a-; and wherein each (Ci-Cg)alkyl, (C2-Cg)alkenyl, (C2-Cg)alkynyl, (C₃C₈)carbocyclyl, (C4-C₈)carbocyclylalkyl, aryl(Cj-Cg)alkyl, heterocyclyl(C]-C_g)alkyl, (Cô C₂ii)aryl, (C2-C2o)heterocyclyl or heteroaryl of each R^c, R^d, R¹, R², R²², R²³, R²⁴,

R⁴, R⁵, R⁶, R⁷, R⁸, R⁹, R¹⁰, R¹¹ or R¹² is, independently, optionally substituted with one or more halo, hydroxy, CN, N3, N(R^a)2>NH(R^a), NH2, NO2, C(O)N(R^a)2, C(O)NH(R^a), C(O)NH2, OC(O)N(R^a)2. OC(O)NH(R^a), 0C(O)NH₂, C(O)OR^a, OC(O)OR^a, C(O)R^a, OC(O)R^a, S(O)nR^a, S(O)2N(R^a)2, S(O)2NH(R^a), S(O)2NH₂, OR^a or R^a. In another aspect, Base is not uracil. In another aspect, the Base is not cytosine.

In another aspect of this embodiment, Base is selected from the group

consisting of

In another aspect of this embodiment, Base is selected from the group

In another embodiment of the method for preparing a compound of Formula 5 IVa or IVb from a compound of Formula V, R¹ is H or CH₃; R² is OR¹ ’ or halogen;

R⁶ is optionally substituted (Cj-Cg)alkyl; one of R^c or R^d is H and the other of R^c or

R^d is optionally substituted (Ci-Cg)alkyl; R²² is OR¹¹, each R⁵, R²³ and R²⁴ is H and Base is selected from the group consisting of:

R⁸ R³ R⁸ R⁸

wherein:

each X¹ is independently N or CR¹⁰;

each X is independently NR , O, or S(O)_n;

eachR⁸ is independently halogen, NR^1!R¹², N(R)OR, NRⁿNRⁿR¹², N₃,

NO, NO₂, CHO, CN, -CH(=NR¹¹), -CH=NNHRⁿ, -CH=N(OR”), -CH(ORⁿ)2, -C(=O)NR¹¹R^!2,-C(=S)NR^ilR¹², -C(=O)OR'^l, (CrCgjalkyl, (C₂-C_g)alkenyl, (C₂

Cg)alkynyl, (C₄-C₈)carbocyclylalkyl, (C_f,-C2o)aryl. (C₂-C₂₀)heterocyclyl, heteroaryl, -C(=O)(C_rC₈)alkyl, -S(O)_n(C]-C₈)alkyl, aryl(C_t-C₈)alkyl, OR¹¹ or SR¹¹;

each n is independently 0, 1, or 2;

each R⁹ or R¹⁰ is independently H, halogen, NR¹ 'R¹², N(Rⁿ)OR^il, NRⁿNR^llR¹², N₃, NO, NO₂, CHO, CN, -CH(=NR¹¹), -CH=NHNR^!i, -CH=N(OR¹¹), -CHfOR¹¹)^ -C(=O)NR“r'², -Ο^ΝΛ¹^¹², -C(=O)ORⁿ, R¹¹, OR¹¹ or SR¹¹;

each R^{l 1} or R¹² is independently H, (Ci-C8)alkyl, (C2-C8)alkenyl, (C2C8)alkynyl, (C3-C8)carbocyclyl, (C4-C8)carbocyclylalkyl, aryl(Ci-Cg)alkyl, heterocyclyl(C[-C8)alkyl, (C6-C2o)aryl, (C2-C2o)heterocyclyl, heteroaryl, -C(=O)(C|C8)alkyl, -8(0),^-C8)alkyl or R¹¹ and R¹² taken together with a nitrogen to which they are both attached form a 3 to 7 membered heterocyclic ring wherein any one carbon atom of said heterocyclic ring can optionally be replaced with -O-, -S(O)n- or -NR^a-; and wherein each (C|-C₈)alkyl, (C₂-C₈)alkenyl, (C₂-C₈)alkynyl, (C₃C₈)carbocyclyl, (C₄-C₈)carbocyclylalkyl, aryl(Ci-C₈)alkyl, heterocyclyl(Cj-C₈)alkyl, (C₆-C₂₀)aryl, (C₂-C₂o)heterocyclyl or heteroaryl of each R^c, R^d, R¹, R², R²², R²³, R²⁴, R⁴, R⁵, R⁶, R⁷, R⁸, R⁹, R¹⁰, R¹¹ or R¹² is, independently, optionally substituted with one or more halo, hydroxy, CN, N3, N(R^a)2. NH(R^a), NH2, NO2, C(O)N(R^a)2, C(O)NH(R³), C(O)NH2, OC(O)N(R^a)2, OC(O)NH(R^a), OC(O)NH2, C(O)OR^a, OC(O)OR^a, C(O)R^a, OC(O)R^a, S(O)nR^a, S(O)₂N(R^a)2, S(O)2NH(R^a), S(O)2NH₂, OR^aor R^a. In another aspect Base is not uracil. In another aspect, the Base is not cytosine.

In another aspect of this embodiment, R¹ is H. In another aspect of this embodiment, R¹ is CH3. In another aspect of this embodiment, R² is F. In another aspect of this embodiment, R² is OH. In another aspect of this embodiment, R²² is OH. In another aspect of this embodiment, each R² and R²² is OH. In another aspect of this embodiment, R⁷ is H. In another aspect of this embodiment, R⁷ is CH3. In another aspect of this embodiment, R⁷ is ethynyl. In another aspect of this embodiment, R⁷ is CN. In another aspect of this embodiment, R⁴ is optionally substituted (C6-C₂₀)aryl. In another aspect of embodiment, R⁴ is optionally substituted phenyl. In another aspect of this embodiment, R⁴ is optionally substituted napthyl. In another aspect of this embodiment, one of R^c or R^d is H and the other of R^c or R^d is CH₃. In another aspect of this embodiment, Ar is optionally substituted nitrophenyl. In another aspect of this embodiment, Base is

. In another o

In another aspect of this aspect of this embodiment, Base is

In another aspect of this embodiment, Base

In another aspect of this embodiment, Base is . In

another aspect of this embodiment, Base is . In another aspect of this embodiment, Base is

In another aspect of this embodiment, Base

In another aspect of this embodiment, Base is another aspect of this embodiment, Base is embodiment, Base is

. In

In another aspect of this

In another aspect of this embodiment, Base

In another aspect of this embodiment, Base is

O

t/VWWL· . In

In another aspect of this another aspect of this embodiment, Base is

In another aspect of this embodiment, Base

In another aspect of this embodiment, Base is

In another embodiment of the method for preparing a compound of Formula la or lb or a pharmaceutically acceptable salt or ester thereof, Formula la is Formula Via, Formula lb is Formula VIb and Formula II is Formula VII:

Formula VII

wherein:

each R¹ is independently H, halogen, optionally substituted (Ci-Cg)alkyl, optionally substituted (C₂ Qialkenyl or optionally substituted (C₂-Cg)alkynyl;

each R is independently halogen or OR ;

each R⁵ is H;

each R²² is OR¹¹ and the remaining variables are defined as for Formulas la or lb or II or Illa or Illb.

In one embodiment of the method for preparing a compound of Formula Via or Formula VIb from a compound of Formula VII, X¹ is CR¹⁰. In another aspect of this embodiment, R¹⁰ is H. In another aspect of this embodiment, R¹ is H. In another aspect of this embodiment, R¹ is F. In another aspect of this embodiment, R¹ is optionally substituted (Ci-Cs)alkyl. In another aspect of this embodiment, R¹ is methyl. In another aspect of this embodiment, R¹ is optionally substituted (C2-Cg)alkenyL In another aspect of this embodiment, R¹ is optionally substituted ethenyl. In another aspect of this embodiment, R¹ is optionally substituted (C2-Cg)alkynyl. In another aspect of this embodiment, R¹ is optionally substituted ethynyl. In another aspect of this embodiment, R is F. In another aspect of this embodiment, R² is OH. In another aspect of this embodiment R²² is OH. In another aspect of this embodiment, each R and R is OH. In another aspect of this embodiment, R⁷ is H. In another aspect of this embodiment, R⁷ is optionally substituted (Ci-Cgjalkyl. In another aspect of this embodiment, R⁷ is optionally substituted (C2-Cg)alkynyl. In another aspect of this embodiment, R⁷ is CN. In another aspect of this embodiment, one of R^c or R^d is H. In another aspect of this embodiment, one of R^c or R^d is H and the other of R^c or R^d is optionally substituted (Ct-Cs)alkyl. In another aspect of this embodiment, R⁶ is optionally substituted (CjC8)alkyl. In another aspect of this embodiment, R⁴ is optionally substituted (C6-C2o)aryl. In another aspect of this embodiment, Ar is optionally substituted nitrophenyl,

In another embodiment of the method for preparing a compound of Formula Via or Formula VIb from a compound of Formula VII, X^l is CH, R¹ is H or CH3 and one of R^c or R^d is H. In another aspect of this embodiment, R² is F. In another aspect of this embodiment, R² is OH. In another aspect of this embodiment R²² is OH. In another aspect of this embodiment, each R² and R²² is OH. In another aspect of this embodiment, R is H. In another aspect of this embodiment, R is optionally substituted (Ci-Cgjalkyl. In another aspect of this embodiment, R⁷ is CH3. In another aspect of this embodiment, R⁷ is optionally substituted (C2-Cg)alkynyl. In another aspect of this embodiment, R⁷ is ethynyl. In another aspect of this embodiment, R⁷ is CN. In another aspect of this embodiment, one of R^c or R^d is H and the other of R^c or R^d is optionally substituted (C|-C/)alkyl. In another aspect of this embodiment, R⁶ is optionally substituted (Ci-Cg)alkyl. In another aspect of this embodiment, R⁴ is optionally substituted ((/, (b0)aryl. In another aspect of this embodiment, Ar is optionally substituted nitrophenyl. In another aspect of this embodiment, R⁸ is NRⁿR¹². In another aspect of this embodiment, R⁸ is OR¹ *. In o another aspect of this embodiment, R is NH₂. In another aspect of this embodiment, R is OH. In another aspect of this embodiment, R is H. In another aspect of this embodiment, R⁹ is NR^{1 l}R¹². In another aspect of this embodiment, R⁹ is OR¹ *. In another aspect of this embodiment, R⁸ is NRⁿR¹² and R⁹ is H. In another aspect of this embodiment, R⁸ is NRⁿR¹² and R⁹ is NRⁿR¹².

In another embodiment of the method for preparing a compound of Formula Via or Formula VIb from a compound of Formula VII, X* is CH, R¹ is H or CH3, one of R^c or R^d is H and R⁷ is CN. In another aspect of this embodiment, R² is F. In another aspect of this embodiment, R² is OH. In another aspect of this embodiment R²² is OH. In another aspect of this embodiment, each R² and R²² is OH. In another aspect of this embodiment, one of R^c or R^d is H and the other of R^c or R^d is optionally substituted (C]-Cg)alkyl. In another aspect of this embodiment, one of R^c or R^d is H and the other of R^c or R^d is CH3. In another aspect of this embodiment, R⁶ is optionally substituted (CrCgJalkyl. In another aspect of this embodiment, R⁴ is optionally substituted (C6-C2o)aryl. In another aspect of this embodiment, R⁴ is optionally substituted phenyl. In another aspect of this embodiment, Ar is optionally substituted nitrophenyl. In another aspect of this embodiment, R⁸ is NR^UR¹². In another aspect of this embodiment, R⁸ is OR¹¹. In another aspect of this embodiment, R⁸ is NH2. In another aspect of this embodiment, R⁸ is OH. In another aspect of this embodiment, R⁹ is H. In another aspect of this embodiment, R⁹ is NR^1!R¹². In another aspect of this embodiment, R⁹ is OR¹ ‘. In another aspect of this embodiment, R⁸ is NR^{1 !}R¹² and R⁹ is H. In another aspect of this embodiment, R⁸ is NH2 and R⁹ is H. In another aspect of this embodiment, R⁸ is NR^llRⁱ² and R⁹ is NR¹ *R¹². In another aspect of this embodiment, R⁸ is NH2 and R⁹ is NH2. In another aspect of this embodiment, R⁸ is OH and R⁹ is NH₂.

In another embodiment, provided is a method of preparing a compound of

Formula Illa or Formula Illb

Formula Illb wherein:

each R^a, R⁴ or R⁶ is independently (Cj-Cg)alkyl, (C₂-Cg)alkenyl, (C₂Cg)alkynyl, (CrCg)carbocyclyl, (C₄-Cg)carbocyclylalkyl, aryl(CrCg)alkyl, heterocycIyl(Ci-Cg)alkyl, (C$--C₂o)aryl, (C₂-C₂o)heterocyclyl or heteroaryl;

each R^c or R^d is independently H, (Ci-Cg)alkyl, (C₂-Cg)alkenyl, (C₂C_a)alkynyl, (Cj-Cglcarbocyclyl, (C4-Cg)carbocyclylalkyl, ary!(Ci-Cg)alkyl, heterocyclyI(Ci-Cg)alkyl, (C₆-C₂₀)aryl, (C₂-C₂₀)heterocyclyl or heteroaryl provided that R^c and R^d are not the same;

each R⁵ is independently H, (CrCe)alkyl, (C₂-C₈)alkenyl, (C₂-C₈)alkynyl, (C₃C₈)carbocyclyl, (Ch-Csjcarbocyclylalkyl, aryl(C|-C₈)aIkyl, heterocyclylfCrC^alkyl, (Ce-C2o)aryl, (C₂-C₂o)heterocyclyl or heteroaryl;

wherein each (C|-C_x)alkvl, (C₂-C₈)alkenyl, (C2-C₈)alkynyl, (C₃C₈)carbocyclyl, (C4~C₈)carbocyclylalkyl, aryl(C]-C₈)alkyl, heterocyclyl(C_rC₈)alkyl, (C6-C₂o)aryl, (Cj-C₂o)hetcrocyclyl or heteroaryl of each R^c, R^d, R⁴, R⁵ or R⁶ is, independently, optionally substituted with one or more halo, hydroxy, CN, N3, N(R^a): NH(R^a), NH2, C{O)N(R^a)2, C(O)NH(R^a), C(O)NH2, OC(O)N(R^a)2, OC(O)NH(R^a), OC(O)NH2, C(O)OR^a, OC(O)OR^a, S(O)nR^a. S(O)₂N(R^a)2, S(O)2NH(R^a), S(O)2NH₂, OR^a or R^a; and each Ar is a (Cf-CFo) aryl or heteroaryl wherein said (C&-C₂o) aryl or heteroaryl is substituted with one or more halogen, NO₂, or (Ci-C₈)haloalkyl and optionally substituted with with one or more CN, N₃, N(R^a)2i C(O)N(R^a)2, OC(O)N(R^a)₂, C(0)OR^a, OC(O)OR^a, C(O)R^a, OC(O)R^a, S(O)nR^a, S(O)2N(R^a)₂, OR^aor R^a with the proviso that Ar is different from R⁴;

said method comprising:

(d) providing a diastereomeric compound of Formula VIII

and (e) crystallizing the compound of Formula VIII from a suitable solvent; thereby forming a pure diasteromer of Formula Illa or Formula 111b.

In one embodiment of the method of preparing a compound of Formula Illa or Formula Illb, R⁵ is H and one of R^c or R^d is H. In another aspect of this embodiment, one of R^c or R^d is H and the other of R^c or R^d is optionally substituted (Cj-C₈)alkyl. In another aspect of this embodiment, one of R^c or R^d is H and the other of R^c or R^d is

C!h. In another aspect of this embodiment, R⁶ is optionally substituted (Cj-C8)alkyl. In another aspect of this embodiment, R⁶ is optionally substituted (Ci-C8) secondary or tertiary alkyl. In another aspect of this embodiment, R⁴ is optionally substituted (C6-C2o)aryl. In another aspect of this embodiment, R⁴ is optionally substituted phenyl. In another aspect of this embodiment, Ar is optionally substituted nitrophenyl. In another aspect of this embodiment, Ar is optionally substituted paranitrophenyl. In another aspect of this embodiment, the chirality at the carbon directly attached to R^c and R^d is 5. In another aspect of this embodiment, the chirality at the carbon directly attached to R^c and R^d is R.

In another embodiment of the method of preparing a compound of Formula Illa or Formula IHb, R^s is H, one of R^c or R^d is H, R⁶ is optionally substituted (CjQjalkyl, and R⁴ is optionally substituted (C6-C2o)aryl. In another aspect of this embodiment, one of R^c or R^d is H and the other of R^c or R^d is optionally substituted (Ci-Cg)alkyl. In another aspect of this embodiment, one of R^c or R^d is H and the other of R^c or R^d is CH3. In another aspect of this embodiment, R⁶ is optionally substituted (Ci-C8) secondary or tertiary alkyl. In another aspect of this embodiment, R⁶ is optionally substituted 2-propyl. In another aspect of this embodiment, R⁴ is optionally substituted phenyl. In another aspect of this embodiment, Ar is optionally substituted nitrophenyl. In another aspect of this embodiment, Ar is optionally substituted para-nitrophenyl. In another aspect of this embodiment, Ar is paranitrophenyl. In another aspect of this embodiment, the chirality at the carbon directly attached to R^c and R^d is S. In another aspect of this embodiment, the chirality at the carbon directly attached to R^c and R^d is R.

In another embodiment of the method of preparing a compound of Formula Illa or Formula IHb, R⁵ is H, one of R^c or R^d is H and the other of R^c or R^d is optionally substituted (C]-C8)alkyl, R⁶ is optionally substituted (C]-C8)alkyl, and R⁴ is optionally substituted phenyl. In another aspect of this embodiment, one of R^c or R^dis H and the other of R^c or R^d is CH3. In another aspect of this embodiment, R⁶ is optionally substituted (Ct-C₈) secondary or tertiary alkyl. In another aspect of this embodiment, R⁶ is optionally substituted 2-propyl. In another aspect of this embodiment, R⁴ is phenyl. In another aspect of this embodiment, Ar is optionally tvJ substituted nitrophenyl. In another aspect of this embodiment, Ar is optionally substituted para-nitrophenyl. In another aspect of this embodiment, Ar is paranitrophenyl. In another aspect of this embodiment, the chirality at the carbon directly attached to R^c and R^d is S. In another aspect of this embodiment, the chirality at the carbon directly attached to R^c and R^d is R.

In another embodiment of the method of preparing a compound of Formula Illa or Formula Illb, R⁵ is H, one of R^c or R^d is H and the other of R^c or R^d is CH₃, R⁶is optionally substituted (Ci-Cg)alkyl, and R⁴ is optionally substituted phenyl. In another aspect of this embodiment, R⁶ is optionally substituted ((/-(/) secondary or tertiary alkyl. In another aspect of this embodiment, R⁶ is optionally substituted 2propyl. In another aspect of this embodiment, R⁶ is 2-propyl. In another aspect of this embodiment, R⁴ is phenyl. In another aspect of this embodiment, Ar is optionally substituted nitrophenyl. In another aspect of this embodiment, Ar is optionally substituted para-nitrophenyl. In another aspect of this embodiment, Ar is paranitrophenyl. In another aspect of this embodiment, the chirality at the carbon directly attached to R^c and R^d is S. In another aspect of this embodiment, the chirality at the carbon directly attached to R^c and R^d is R.

The diastereomeric mixture of the compound of Formula VIII is resolved by crystallization of the compound of Formula VIII from a suitable solvent. Nonlimiting examples of suitable solvents are diethyl ether, dipropyl ether, di i-butyl ether, methyl /-butyl ether, (/-C/ halogenated alkanes, (/-(/ hydrocarbon, tetrahydro furan, toluene, xylene, dioxane and the like. In another embodiment, the compound of Formula IV is dissolved in a suitable solvent and crystallization is induced by addition of a (/-(/ hydrocarbon or (/-(/ cyclic hydrocarbon. In a preferred embodiment, the compound of Formula VIII is dissolved in an ether solvent and crystallization is induced by addition of a (/-(/ hydrocarbon. In a particularly preferred embodiment, the compound of Formula VIII is dissolved in diethyl ether and crystallization is induced by the addition of hexane.

The diastereomeric mixture of the compound of Formula VIII is resolved by crystallization of the compound of Formula VIII from a suitable solvent at a temperature of about 80 °C to about -20 °C. Preferrably, the temperature is about 30 °C to about -20 °C, more preferably about ambient to -IO °C.

The diastereomeric mixture of the compound of Formula VIII is resolved by crystallization of the compound of Formula VIII from a suitable solvent wherein the concentration of the compound of Formula VIII in solution is about 25 g to about 1000 g per liter of solvent. More typically, the concentration of the compound of Formula VIII is about 50 to 500 g per liter of solvent.

The resolution of the diastereomeric mixture of the compound of Formula VIII by crystallization may be promoted by the addition of seed crystals of the pure diastereomer. Seed crystals of pure diastereomers may be obtained through purification of the diastereomeric mixture of the compound of Formula VIII by liquid chromatography, chiral liquid chromatography, high pressure liquid chromatography, or chiral high pressure liquid chromatography such as by the non-limiting methods described herein.

Typically, the crystallization of the diastereomeric mixture of the compound of Formula VIII produces a mixture of diastereomers containing at least 60 % of a single diastereomer. More typically, the mixture produced contains at least 70 % of a single diastereomer, most typically, at least 80 % of a single diastereomer, preferably at least 90 % of a single diastereomer, and more preferably at least 95 % of a single diastereomer. Higher diastereomeric purity, for example at least 99 % diastereomeric purity, may be obtained by one or more subsequent crystallizations. The yield of crystalline material from a single crystallization is typically about 10 to 45 %, more typically about 20-35 %.

In another embodiment, provided is a compound of Formula Illa or Formula Illb

or a salt or ester thereof;

wherein:

each R^a, R⁴ or R⁶ is independently (Ci-Cg)alkyl, (C₂-C₈)alkenyl, (C₂C₈)alkynyl, (C3-C₈)carbocyclyl, (C₄-C₈)carbocyclylalkyl, aryl(Ci-C₈)alkyl, heterocyclyl(Ci-C₈)alkyl, (Ce-C₂o)aryl, (C₂-C₂o)heterocyclyl or heteroaryl;

each R^c or R^d is independently H, (Ci-C₈)alkyl, (C₂-C₈)alkenyl, (C₂C₈)alkynyl, (C3-C₈)carbocyclyl, (C₄--C₈)carbocyclylalkyl, aryl(Ci-C₈)alkyl, heterocyclyl(Ci-C₈)alkyl, (C6-C₂o)aryl, (C₂~C₂o)heterocyclyl or hetero aryl provided that R^c and R^d are not the same;

each R⁵ is independently H, (C]-C₈)alkyl, (C₂-C₈)alkenyl, (C₂-C₈)alkynyl, (C₃C₈)carbocyclyl, (C₄-C₈)carbocyclylalkyl, aryl(C]-C₈)aIkyl, heterocyclyl(Ci-C₈)alkyl, (C₆-C₂o)aryl, (C₂-C2o)heterocyclyl or heteroaryl;

wherein each (Ci-C₈)alkyl, (C₂-C₈)alkenyl, (C₂-C₈)alkynyl, (C₃C₈)carbocyclyl, (C₄-C₈)carbocyclylalkyl, aryl(C|-C₈)alkyl, heterocyclyl(Ci-C₈)alkyl, (C₆-C₂o)aryl, (C₂-C₂o)heterocyclyl or heteroaryl of each R^c, R^d, R⁴, R⁵ or R⁶ is, independently, optionally substituted with one or more halo, hydroxy, CN, N3, N(R^a)2, NH(R^a), NH₂, C(O)N(R^a)2, C(O)NH(R^a), C(O)NH2, OC(O)N(R^a)_z, OC(O)NH(R^a), OC(O)NH2, C(O)OR^a, OC(O)OR^a, S(O)nR^a, S(O)₂N(R^a)2, S(O)2NH(R^a), S(O)2NH₂, OR^a or R^a; and each Ar is a (Q-fXo) aryl or heteroaryl wherein said (C6~C₂o) aryl or heteroaryl is substituted with one or more halogen, NO₂, or (Ci-C₈)haloalkyl and optionally substituted with with one or more CN, N₃, N(R^a)2, C(O)N(R^a)2, OC(O)N(R^a)2, C(O)OR^a, OC(0)OR^a, C(O)R^a, OC(O)R^a, S(O)nR^a, S(O)2N(R^a)2, OR^aor R^a with the proviso that Ar is different from R⁴. y «

In another embodiment of the compound of Formula Illa or Formula lllb, R⁵is H and one of R^c or R^d is H. In another aspect of this embodiment, one of R^c or R^dis H and the other of R^c or R^d is optionally substituted (Cj-Ceialkyl. In another aspect of this embodiment, one of R^c or R^d is H and the other of R^c or R^d is CH₃. In another aspect of this embodiment, R⁶ is optionally substituted (Ci-Cg)alkyl. In another aspect of this embodiment, R⁶ is optionally substituted (Cj-Cg) secondary or tertiary alkyl. In another aspect of this embodiment, R⁴ is optionally substituted ((/ C/o)ary!. In another aspect of this embodiment, R⁴ is optionally substituted phenyl. In another aspect of this embodiment, Ar is optionally substituted nitrophenyl. In another aspect of this embodiment, Ar is optionally substituted pura-nitrophenyl. In another aspect of this embodiment, the chirality at the carbon directly attached to R^c and R^d is 5. In another aspect of this embodiment, the chirality at the carbon directly attached to R^cand R^d is R. In another aspect of this embodiment, the moiety

of Formula Illa or Formula lllb comprises a nitro gen-linked ester of a naturally occurring a-amino acid.

In another embodiment of the compound of Formula Illa or Formula lllb, R⁵is H, one of R^c or R^d is H, R⁶ is optionally substituted (Ci-Cg)alkyl and R⁴ is optionally substituted (C6-C2o)aryl. In another aspect of this embodiment, one of R^cor R^d is H and the other of R^c or R^d is optionally substituted (C]-Cg)alkyl. In another aspect of this embodiment, one of R^c or R^d is H and the other of R^c orR^d is CH3. In another aspect of this embodiment, R⁶ is optionally substituted (Ci-Cg) secondary or tertiary alkyl. In another aspect of this embodiment, R⁶ is optionally substituted 2propyl. In another aspect of this embodiment, R⁴ is optionally substituted phenyl. In another aspect of this embodiment, Ar is optionally substituted nitrophenyl. In another aspect of this embodiment, Ar is optionally substituted para-nitrophenyl. In another aspect of this embodiment, Ar is para-nitrophenyl. In another aspect of this embodiment, the chirality at the carbon directly attached to R^c and R^d is 5. In another aspect of this embodiment, the chirality at the carbon directly attached to R^c and R^d is

R. In another aspect of this embodiment, the moiety

R⁵

R‘ of Formula Illa or Formula Illb comprises a nitrogen-linked ester of a naturally occurring α-amino acid.

In another embodiment of the compound of Formula Illa or Formula Illb, R⁵is H, one of R^c or R^d is H and the other of R^c or R^d is optionally substituted (C_rC₈)alkyl, R⁶ is optionally substituted (Ci-Cs)alkyl and R⁴ is optionally substituted phenyl. In another aspect of this embodiment, one of R^c or R^d is H and the other of R^cor R^d is CH ;. In another aspect of this embodiment, R⁶ is optionally substituted (Cj Cg) secondary or tertiary alkyl. In another aspect of this embodiment, R⁶ is optionally substituted 2-propyl. In another aspect of this embodiment, R⁴ is phenyl. In another aspect of this embodiment, Ar is optionally substituted nitrophenyl. In another aspect of this embodiment, Ar is optionally substituted para-nitrophenyl. In another aspect of this embodiment, Ar is para-nitrophenyl. In another aspect of this embodiment, the chirality at the carbon directly attached to R^c and R^d is 5. In another aspect of this embodiment, the chirality at the carbon directly attached to R^c and R^d is R. In another aspect of this embodiment, the moiety

R' of Formula Illa or Formula Illb comprises a nitrogen-linked ester of a naturally occurring α-amino acid.

In another embodiment of the compound of Formula Illa or Formula Illb, R⁵is H, one of R^c or R^d is H and the other of R^c or R^d is CH3, R⁶ is optionally substituted (Ci-C8)alkyl, and R⁴ is optionally substituted phenyl. In another aspect of this embodiment, R⁶ is optionally substituted (Ci-C₈) secondary or tertiary alkyl. In another aspect of this embodiment, R⁶ is optionally substituted 2-propyl. In another *

aspect of this embodiment, R⁶ is 2-propyl. In another aspect of this embodiment, R⁴ is phenyl. In another aspect of this embodiment, Ar is optionally substituted nitrophenyl. In another aspect of this embodiment, Ar is optionally substituted paranitrophenyl. In another aspect of this embodiment, Ar is para-nitrophenyl. In another 5 aspect of this embodiment, the chirality at the carbon directly attached to R^c and R^d is

S. In another aspect of this embodiment, the chirality at the carbon directly attached to R^c and R^d is R. In another aspect of this embodiment, the moiety

of Formula Illa or Formula Illb comprises a nitrogen-linked ester of a naturally occurring α-amîno acid.

In another embodiment, provided are compounds of Formula Illa or Formula

IHb selected from the group consisting of:

esters thereof.

In another embodiment, provided is a method of preparing a compound of

Formula VIII

Formula VIII wherein each R^a, R⁴ or R⁶ is independently (C|-C₈)alkyl, (C₂-C₈)alkenyl, (C₂C₈)alkynyl, (Cv(^)carbocyclyl, (C._; -Cg)carbocyclylalkyl, aryl(C]-Cg)alkyl, heterocyclyl(C]-C₈)alkyl, (Cô-C^ojaryl, (C₂-C₂o)heterocyclyl or heteroaryl;

each R^c or R^d is independently II, (C_t-C₈)alkyl, (C₂-C₈)alkenyl, (C₂C₈)alkynyl, (C₃-C₈)carbocyclyl, (Q-Cx)carbocyclylalkyl, aryl(Ci-Cg)alkyl, heterocyclyI(Ci-C_s)alkyl, (C₆-C₂o)aryl, (C₂-C₂₀)heterocyclyl or heteroaryl provided that R^c and R^d are not the same;

each R⁵ is independently H, (Ci-Cg)alkyl, (C₂-C₈)alkenyl, (C₂-Cg)alkynyl, (C₃C₈)carbocyclyl, (C₄-C₈)carbocyclylalkyl, aryl(Cj-C₈)alkyl, heterocyclyl(Ci-C₈)alkyl, (C6-C₂o)aryl, (C₂-C₂o)heterocyclyl or heteroaryl;

wherein each (Ci-Cg)alkyl, (C₂-Cg)alkenyl, (C₂-Cg)alkynyl, (C₃C₈)carbocyclyl, (C₄-Cg)carbocyclylalkyl, aryl(C]-C₈)alkyl, heterocyclyl(C[-C₈)aIkyl, (C6-C₂o)aryl, (C₂-C₂o)heterocyclyl, heteroaryl of each R^c, R^d, R⁴, R⁵ or R⁵ is, independently, optionally substituted with one or more halo, hydroxy, CN, N3, N(R^a)2, NH(R^a), NH2, C(O)N(R^a)2, C(O)NH(R^a), C(O)NH2, OC(O)N(R^a)2, OC(O)NH(R^a), OC(O)NH2, C(O)OR^a, OC(O)OR^a, S(O)nR^a, S(O)₂N(R^a)2, S(O)2NH(R^a), S(O)2NH₂, OR^a or R^a; and each Ar is a (Cè-C₂o) aryl or heteroaryl wherein said (Cô-C₂o) aryl or heteroaryl is substituted with one or more halogen, NO₂, or (Ci-C₈)haloalkyl and optionally substituted with with one or more CN, N₃, N(R^a)2, C(O)N(R^a)2, OC(O)N(R^a)₂, C(O)OR^a, OC(O)OR^a, C(O)R^a, OC(O)R^a, S(O)nR^a, S(O)2N(R^a)₂, OR^aor R^a with the proviso that Ar is different from R⁴;

said method comprising:

(f) providing a chirally pure amino acid ester of Formula IX or a salt thereof

Formula IX (g) treating the compound of Formula IX with a compound of Formula X in the presence of a base

Formula X wherein each X³ is halogen; and (h) treating the resulting mixture with ArOH; thereby forming a compound of Formula VIII. Typically, the chirally pure amino acid of Formula IX or a salt thereof is dissolved or suspended in a suitable non-nucleophilic solvent. Non-limiting nonnucleophilic solvents include haloalkanes, e.g., methylene chloride, dichloroethane and ethers, e.g. dioxane, tetrahydrofuran and glymes. Typically, the suspension or solution contains about 0.1 to about 5 moles of the compound of Formula IX per liter of solvent.

The suspension or solution of the chirally pure amino acid of Formula IX is treated with a compound of Formula X. Typically, the reaction is conducted at about -20 to about 60 °C. The mole ratio of the compound of Formula IX to the compound of Formula X is about 1:2 to about 2:1, preferably about 1 ; 1. The reaction is conducted in the presence of a non-nucleophilic base. Non-limiting examples of nonnucleophilic bases are tertiary amines, e.g. triethylamine, diisopropylethylamine and tri ethylamine; metal hydrides, e.g. LiH, NaH and CalF; and nitrogen containing heterocycles, e.g. pyridine and dimethylaminopyridine. In a preferred embodiment, the base is a tertiary amine such as triethylamine. When the compound of Formula IX is a salt of a mono-protic acid, the mole ratio of base to the compound of Formula IX is typically about 2:1. If the compound of Formula IX is a free base, the mole ratio of base to the compound of Formula IX is about 1:1.

The reaction of the compound of Formula IX with the compound of Formula X may be followed by many conventional means known to those skilled in the art. mA”

Such means include thin-layer chromatography and hplc. When the reaction between the compound Formula IX and the compound of Formula X is complete, the reaction is treated with a phenolic compound ArOH where Ar is defined as herein. The mole ratio of the compound of Formula X to ArOH is about 1.1:1 to about 1:1.1, preferably about 1:1. After the addition of ArOH, additional base is required, typically enough base to neutralize the acid generated in the reaction. Typically, the additional base is a non-nucleophilic base such as described above.

The compound of Formula VIII is isolated by conventional means known to those skilled in the art. For example, the salt formed in the reaction may be precipitated from the reaction mixture and the compound of Formula VIII isolated by evaporation of the solvent followed by crystallization or chromatography.

In one embodiment of the method of preparing a compound of Formula VIII, R⁵ is H and one of R^c or R^d is H. In another aspect of this embodiment, one of R^c or R^d is H and the other of R^c or R^d is optionally substituted (Ci-Cg)alkyl. In another aspect of this embodiment, one of R^c or R^d is H and the other of R^c or R^d is CHj. In another aspect of this embodiment, R⁶ is optionally substituted (Ci-Cg)alkyl. In another aspect of this embodiment, R⁶ is optionally substituted (Ci-C₈) secondary or tertiary alkyl. In another aspect of this embodiment, R⁴ is optionally substituted (Cô-CioJaryL In another aspect of this embodiment, R⁴ is optionally substituted phenyl. In another aspect of this embodiment, Ar is optionally substituted nitrophenyl. In another aspect of this embodiment, Ar is optionally substituted paranitrophenyl. In another aspect of this embodiment, the chirality at the carbon directly attached to R^c and R^d is S. In another aspect of this embodiment, the chirality at the carbon directly attached to R^c and R^d is R. In another aspect of this embodiment, the compound of Formula IX or salt thereof, is an ester of a naturally occurring a-amino acid.

In another embodiment of the method of preparing a compound of Formula VIII, R⁵ is H, one of R^c or R^d is H, R⁶ is optionally substituted (Ci-Cg)alkyl, and R⁴ is optionally substituted (Q-Ciojaryl. In another aspect of this embodiment, one of R^cor R^d is H and the other of R^c or R^d is optionally substituted (Ci-Cg)alkyl. In another aspect of this embodiment, one of R^c or R^d is H and the other of R^c or R^d is CH.3. In another aspect of this embodiment, R⁶ is optionally substituted (Cj-Cg) secondary or tertiary alkyl. In another aspect of this embodiment, R⁶ is optionally substituted 2propyl. In another aspect of this embodiment, R⁴ is optionally substituted phenyl. In another aspect of this embodiment, Ar is optionally substituted nitrophenyl. In another aspect of this embodiment, Ar is optionally substituted para-nitrophenyl. In another aspect of this embodiment, Ar is pora-nitrophenyl. In another aspect of this embodiment, the chirality at the carbon directly attached to R^c and R^d is S. In another aspect of this embodiment, the chirality at the carbon directly attached to R^c and R^d is R. In another aspect of this embodiment, the compound of Formula IX or salt thereof, is an ester of a naturally occurring a-amino acid.

In another embodiment of the method of preparing a compound of Formula VIII, R⁵ is H, one of R^c or R^d is H and the other of R^c or R^d is optionally substituted (Ci-Cg)alkyl, R⁶ is optionally substituted (Ci-Cg)alkyl, and R⁴ is optionally substituted phenyl. In another aspect of this embodiment, one of R^c or R^d is H and the other of R^cor R^d is CH3. In another aspect of this embodiment, R⁶ is optionally substituted (Cr Cg) secondary or tertiary alkyl. In another aspect of this embodiment, R⁶ is optionally substituted 2-propyl. In another aspect of this embodiment, R⁴ is phenyl. In another aspect of this embodiment, Ar is optionally substituted nitrophenyl. In another aspect of this embodiment, Ar is optionally substituted para-nitrophenyl. In another aspect of this embodiment, Ar is para-nitrophenyl. In another aspect of this embodiment, the chirality at the carbon directly attached to R^c and R^d is 5. In another aspect of this embodiment, the chirality at the carbon directly attached to R^c and R^d is R. In another aspect of this embodiment, the compound of Formula IX or salt thereof, is an ester of a naturally occurring α,-amino acid.

In another embodiment of the method of preparing a compound of Formula VIII, R^s is H, one of R^c or R^d is H and the other of R^c or R^d is CH₃, R⁶ is optionally substituted (Ci-Cg)alkyl, and R⁴ is optionally substituted phenyl. In another aspect of this embodiment, R⁶ is optionally substituted (C|-Cg) secondary or tertiary alkyl. In another aspect of this embodiment, R⁶ is optionally substituted 2-propyl. In another aspect of this embodiment, R⁶ is 2-propyl. In another aspect of this embodiment, R⁴ is phenyl. In another aspect of this embodiment, Ar is optionally substituted nitrophenyl. In another aspect of this embodiment, Ar is optionally substituted paranitrophenyl. In another aspect of this embodiment, Ar is para-nitrophenyl. In another aspect of this embodiment, the chirality at the carbon directly attached to R^c and R^d is .S'. In another aspect of this embodiment, the chirality at the carbon directly attached 5 to R^c and R^d is R. In another aspect of this embodiment, the compound of Formula IX or salt thereof, is an ester of a naturally occurring α-amino acid.

In another embodiment of the method for preparing a compound of Formula la or lb or a pharmaceutically acceptable salt or ester thereof, Formula la is Formula Xia, Formula lb is Formula Xlb and Formula II is Formula XII:

Formula XII wherein:

each R¹ is independently H, halogen, optionally substituted (Cj-C_s)alkyl, optionally substituted ((/(Dalkcnyl or optionally substituted ((/ (/ialkynyl;

I i each R is independently halogen or OR ;

each R⁵ is H;

In one embodiment of the method for preparing a compound of Formula Xia or Formula Xlb from a compound of Formula XII, X¹ is CR¹⁰. In another aspect of this embodiment, R¹⁰ is H. In another aspect of this embodiment, R¹ is H. In another aspect of this embodiment, R¹ is F. In another aspect of this embodiment, R^l is optionally substituted (Ci-Cg)alkyl. In another aspect of this embodiment, R¹ is methyl. In another aspect of this embodiment, R¹ is optionally substituted ((/-(/ialkenyl. In another aspect of this embodiment, R¹ is optionally substituted ethenyl. In another aspect of this embodiment, R¹ is optionally substituted (C2-C₈)alkynyl. In another aspect of this embodiment, R¹ is optionally substituted ethynyl. In another aspect of this embodiment, R is F. In another aspect of this embodiment, R² is OH. In another aspect of this embodiment R²² is OH. In another aspect of this embodiment, each R and R is OH. In another aspect of this embodiment, each R² is F and R²² is OH. In another aspect of this embodiment, R⁷ is

H. In another aspect of this embodiment, R⁷ is optionally substituted (Ci-Cg)alkyl. In another aspect of this embodiment, R⁷ is optionally substituted (C2-Cg)alkynyl. In another aspect of this embodiment, R⁷ is CN. In another aspect of this embodiment, one of R^c or R^d is H. In another aspect of this embodiment, one of R^c or R^d is H and the other of R^c or R^d is optionally substituted (Ct-Cg)alkyl. In another aspect of this embodiment, R⁶ is optionally substituted (Ci-Cg)alkyl. In another aspect of this embodiment, R⁴ is optionally substituted (C6-C?())aryl. In another aspect of this embodiment, Ar is optionally substituted nitrophenyl.

In another embodiment of the method for preparing a compound of Formula Xia or Formula Xlb from a compound of Formula XII, X¹ is CH, R¹ is H or CH3 and one of R^c or R^d is H. In another aspect of this embodiment, R² is F. In another aspect of this embodiment, R is OH. In another aspect of this embodiment R is OH. In another aspect of this embodiment, each R² and R²² is OH. In another aspect of this embodiment, each R is F and R is OH. In another aspect of this embodiment, R is H. In another aspect of this embodiment, R is optionally substituted (Ci-Cejalkyl. In another aspect of this embodiment, R⁷ is CH3. In another aspect of this embodiment, R⁷ is optionally substituted (C2-Cg)alkynyl. In another aspect of this embodiment, R⁷ is ethynyl. In another aspect of this embodiment, R⁷ is CN. In another aspect of this embodiment, one of R^c or R^d is H and the other of R^c or R^d is optionally substituted (Ci-Cg)alkyl. In another aspect of this embodiment, R⁶ is optionally substituted (Ci-Cg)alkyl. In another aspect of this embodiment, R⁴ is optionally substituted (C₍₎ - C/o)aryl. In another aspect of this embodiment, Ar is optionally substituted nitrophenyl. In another aspect of this embodiment, R is NR¹ *R¹². In another aspect of this embodiment, R⁸ is OR¹¹. In another aspect of this embodiment, R⁸ is Nil- In another aspect of this embodiment, R⁸ is OH. In another aspect of this embodiment, R⁹ is H. In another aspect of this embodiment, R⁹ is NR^{1 !}R^l2. In another aspect of this embodiment, R⁹ is OR¹¹. In another aspect of this embodiment, R⁸ is NRⁿR¹² and R⁹ is H. In another aspect of this embodiment, R⁸ is NR¹’R¹² and R⁹ is NRⁿR¹².

In another embodiment of the method for preparing a compound of Formula XIa or Formula Xlb from a compound of Formula XII, X¹ is CH, R¹ is H or CH₃, one of R^c or R^d is H and R⁷ is H. In another aspect of this embodiment, R² is F. In another aspect of this embodiment, R² is OH. In another aspect of this embodiment R²² is OH. In another aspect of this embodiment, each R² and R²² is OH. In another aspect of this embodiment, each R² is F and R²² is OH. In another aspect of this embodiment, one of R^c or R^d is H and the other of R^c or R^d is optionally substituted (Ci-Cs)alkyl. In another aspect of this embodiment, one of R^c or R^d is H and the other of R^c or R^d is CH3. In another aspect of this embodiment, R⁶ is optionally substituted (Ci-Cg)alkyl. In another aspect of this embodiment, R⁴ is optionally substituted (C6-C2o)aryl. In another aspect of this embodiment, R⁴ is optionally substituted phenyl. In another aspect of this embodiment, Ar is optionally substituted nitrophenyl. In another aspect of this embodiment, R⁸ is NR¹ *R¹². In another aspect of this embodiment, R⁸ is OR¹¹. In another aspect of this embodiment, R⁸ is NIL. In another aspect of this embodiment, R is OH. In another aspect of this embodiment, R is H. In another aspect of this embodiment, R⁹ is NRⁿR¹². In another aspect of this embodiment, R⁹ is OR¹¹. In another aspect of this embodiment, R⁸ is NRⁿR¹² and R⁹isH. In another aspect of this embodiment, R is NH2 and R is H. In another aspect of this embodiment, R⁸ is NR¹ *R¹² and R⁹ is NRⁿR¹². In another aspect of this embodiment, R⁸ is NH2 and R⁹ is NH2. In another aspect of this embodiment, R⁸ is OR^{1 !} and R⁹ is NH2. In another aspect of this embodiment, R⁸ is OH and R⁹ is NH₂.

In one embodiment of the method for preparing a compound of Formula Xia or Formula Xlb from a compound of Formula XII, X¹ is N. In another aspect of this embodiment, R¹ is H. In another aspect of this embodiment, R^l is F. In another aspect of this embodiment, R¹ is optionally substituted (Ci-Cg)alkyl. In another aspect of this embodiment, R¹ is methyl. In another aspect of this embodiment, R¹ is optionally substituted (C2-Cg)alkenyl. In another aspect of this embodiment, R¹ is optionally substituted ethenyl. In another aspect of this embodiment, R¹ is optionally substituted (C2Cg)aIkynyl. In another aspect of this embodiment, R¹ is optionally substituted ethynyl. In another aspect of this embodiment, R² is F. In another aspect of this embodiment, R² is OH. In another aspect of this embodiment R²² is OH. In another aspect of this embodiment, each R and R is OH. In another aspect of this embodiment, each R² is F and R²² is OH. In another aspect of this embodiment, R⁷ is

H. In another aspect of this embodiment, R⁷ is optionally substituted (Ci-C8)alkyl. In another aspect of this embodiment, R⁷ is optionally substituted (C2-C8)alkynyl. In another aspect of this embodiment, R⁷ is CN. In another aspect of this embodiment, one of R^c or R^d is H. In another aspect of this embodiment, one of R^c or R^d is H and the other of R^c or R^d is optionally substituted (C]-C8)alkyl. In another aspect of this embodiment, R⁶ is optionally substituted (Ci-Cg)alkyl. In another aspect of this embodiment, R⁴ is optionally substituted (G, C?(;)aryl. In another aspect of this embodiment, Ar is optionally substituted nitrophenyl.

In another embodiment of the method for preparing a compound of Formula Xia or Formula Xlb from a compound of Formula XII, X¹ is N, R¹ is H or Cll· and one of R^c or R^d is H. In another aspect of this embodiment, R² is F. In another aspect of this embodiment, R² is OH. In another aspect of this embodiment R²² is OH. In another aspect of this embodiment, each R² and R²² is OH. In another aspect of this embodiment, each R is F and R is OH. In another aspect of this embodiment, R is H. In another aspect of this embodiment, R is optionally substituted (Ci-Cg)alkyl. In another aspect of this embodiment, R⁷ is CH3. In another aspect of this embodiment, R is optionally substituted (C2-C8)alkynyl. In another aspect of this embodiment, R is ethynyl. In another aspect of this embodiment, R is CN. In another aspect of this embodiment, one of R^c or R^d is H and the other of R^c or R^d is optionally substituted (C]-C8)alkyl. In another aspect of this embodiment, R⁶ is optionally substituted (C[-C8)alkyl. In another aspect of this embodiment, R⁴ is optionally substituted (C6-C2o)aryl. In another aspect of this embodiment, Ar is optionally substituted nitrophenyl. In another aspect of this embodiment, R⁸ is NR^{1 l}R¹². In another aspect of this embodiment, R⁸ is OR^{1 !}. In another aspect of this embodiment, R is NH2. In another aspect of this embodiment, R is OH. In another aspect of this embodiment, R⁹ is H. In another aspect of this embodiment, R⁹ is NRⁿR¹². In another aspect of this embodiment, R⁹ is OR¹¹. In another aspect of this embodiment, R is NR R and R isH. In another aspect of this embodiment, R is NR^llR¹² and R⁹ is NR^1!R¹².

In another embodiment of the method for preparing a compound of Formula Xia or Formula Xlb from a compound of Formula XII, X¹ is N, R¹ is H or CH₃, one ί of R^c or R^d is H and R⁷ is H. In another aspect of this embodiment, R² is F. In another aspect of this embodiment, R² is OH. In another aspect of this embodiment R²² is OH. In another aspect of this embodiment, each R² and R²² is OH. In another aspect of this embodiment, each R² is F and R²² is OH. In another aspect of this embodiment, one of R^c or R^d is H and the other of R^c or R^d is optionally substituted (Ci-Cg)alkyl. In another aspect of this embodiment, one of R^c or R^d is H and the other of R^c or R^d is CH3. In another aspect of this embodiment, R⁶ is optionally substituted (Cj-Cg)alkyL In another aspect of this embodiment, R⁴ is optionally substituted ( Ct)-C2(;)aryl. In another aspect of this embodiment, R⁴ is optionally substituted phenyl. In another aspect of this embodiment, Ar is optionally substituted nitrophenyl. In another aspect of this embodiment, R⁸ is NR^{1 !}R¹². In another aspect of this embodiment, R is OR . In another aspect of this embodiment, R is NH2. In another aspect of this embodiment, R⁸ is OH. In another aspect of this embodiment, R⁹ is H. In another aspect of this embodiment, R⁹ is NR^llR¹². In another aspect of this embodiment, R⁹ is OR¹ '. In another aspect of this embodiment, R⁸ is NRⁿR¹² and R⁹is H. In another aspect of this embodiment, R is NH2 and R is H. In another aspect of this embodiment, R⁸ is NR^{1 ]}R¹² and R⁹ is NR^{1 !}R¹². In another aspect of this embodiment, R⁸ is NH2 and R⁹ is NH2. In another aspect of this embodiment, R⁸ is OR¹¹ and R⁹ is NH2. In another aspect of this embodiment, R⁸ is OH and R⁹ is NH2.

In another embodiment of the method for preparing a compound of Formula la or lb or a pharmaceutically acceptable salt or ester thereof, Formula la is Formula Xllla, Formula lb is Formula XHIb and Formula II is Formula XIV:

Formula XHIa

R²² R²

Formula XHIb

R^a

R²² R²

Formula XIV wherein:

each R¹ is independently H, halogen, optionally substituted (Cj-C₈)alkyl, optionally substituted (C₂-C₈)alkenyl or optionally substituted (C₂ —Cslalkynyl;

each R² is independently halogen or OR¹¹;

each R⁵ is H;

each R²² is OR¹¹ and the remaining variables are defined as for Formulas la or lb or II or Illa or Hlb.

In one embodiment of the method for preparing a compound of Formula Xllla or Formula XHIb from a compound of Formula XIV, X¹ is CR¹⁰. In another aspect of this embodiment, R¹⁰ is H. In another aspect of this embodiment, Rⁱ⁰ is CH₃. In another aspect of this embodiment, R¹ is H. In another aspect of this embodiment, R¹is F. In another aspect of this embodiment, R¹ is optionally substituted (Ci-Cg)alkyl. In another aspect of this embodiment, R¹ is methyl. In another aspect of this embodiment, R^l is optionally substituted (C₂-C₈/alkenyl. In another aspect of this embodiment, R¹ is optionally substituted ethenyl. In another aspect of this embodiment, R¹ is optionally substituted ((/-(//alkynyl. In another aspect of this embodiment, R¹ is optionally substituted ethynyl. In another aspect of this embodiment, R is F. In another aspect of this embodiment, R is OH. In another aspect of this embodiment R²² is OH. In another aspect of this embodiment, each R²

Λ A A 77 and R is OH. In another aspect of this embodiment, each R is F and R is OH. In another aspect of this embodiment, R is H. In another aspect of this embodiment, R is optionally substituted ((/(//alkyl. In another aspect of this embodiment, R⁷ is optionally substituted (C2-C8)alkynyl. In another aspect of this embodiment, R⁷ is CN. In another aspect of this embodiment, one of R^c or R^d is H. In another aspect of this embodiment, one of R^c or R^d is H and the other of R^c or R^d is optionally substituted (CrCg/alkyl. In another aspect of this embodiment, R⁶ is optionally substituted (C|-C8/alkyl. In another aspect of this embodiment, R⁴ is optionally substituted (C₆-C₂o)aryl. In another aspect of this embodiment, Ar is optionally substituted nitrophenyl.

In another embodiment of the method for preparing a compound of Formula Xllla or Formula XHIb from a compound of Formula XIV, X¹ is CH, R¹ is H or CH3 and one of R^c or R^d is H. In another aspect of this embodiment, R² is F. In another aspect of this embodiment, R is OH. In another aspect of this embodiment R is OH. In another aspect of this embodiment, each R² and R²² is OH. In another aspect of this embodiment, each R² is F and R²² is OH. In another aspect of this embodiment, R is H. In another aspect of this embodiment, R is optionally substituted ((/ (//alkyl. In another aspect of this embodiment, R⁷ is CH3. In another aspect of this embodiment, R⁷ is optionally substituted (C₂-C₈)alkynyl. In another aspect of this embodiment, R⁷ is ethynyl. In another aspect of this embodiment, R⁷ is CN. In another aspect of this embodiment, one of R^c or R^d is H and the other of R^c or R^d is optionally substituted (Cj-Cs)alkvl. In another aspect of this embodiment, R⁶ is optionally substituted (Cj-Cgjalkyl. In another aspect of this embodiment, R⁴ is optionally substituted (0, (%)aryl. In another aspect of this embodiment, Ar is optionally substituted nitrophenyl. In another aspect of this embodiment, R⁸ is NR¹ ’R¹². In another aspect of this embodiment, R⁸ is OR^{1 !}. In another aspect of this embodiment, R⁸ is NH₂. In another aspect of this embodiment, R⁸ is OH.

In another embodiment of the method for preparing a compound of Formula Xllla or Formula XHIb from a compound of Formula XIV, X¹ is CH, R¹ is H or CH3, one of R^c or R^d is H and R⁷ is H. In another aspect of this embodiment, R² is F. In another aspect of this embodiment, R² is OH. In another aspect of this embodiment R is OH. In another aspect of this embodiment, each R and R is OH. In another aspect of this embodiment, each R² is F and R²² is OH. In another aspect of this embodiment, one of R^c or R^d is H and the other of R^c or R^d is optionally substituted (Cj-Cg)alkyl. In another aspect of this embodiment, one of R^c or R^d is H and the other of R^c or R^d is CH3. In another aspect of this embodiment, R⁶ is optionally substituted (Ci-Cg)alkyl. In another aspect of this embodiment, R⁴ is optionally substituted (C&-C2o)aryl. In another aspect of this embodiment, R⁴ is optionally substituted phenyl. In another aspect of this embodiment, Ar is optionally substituted nitrophenyl. In another aspect of this embodiment, R⁸ is NRⁿR¹². In another aspect of this embodiment, R⁸ is OR¹ ’. In another aspect of this embodiment, R⁸ is NH2. In another

Q aspect of this embodiment, R is OH.

In one embodiment of the method for preparing a compound of Formula Xllla or Formula XHIb from a compound of Formula XIV, X¹ is CF. In another aspect of this embodiment, R¹ is H. In another aspect of this embodiment, R¹ is F. In another aspect of this embodiment, R¹ is optionally substituted (Ci-Cg)alkyl. In another aspect of this embodiment, R^l is methyl. In another aspect of this embodiment, R¹ is optionally substituted (C2-Cg)alkenyl. In another aspect of this embodiment, R¹ is optionally substituted ethenyl. In another aspect of this embodiment, R¹ is optionally substituted (C2-C₈)alkynyl. In another aspect of this embodiment, R¹ is optionally substituted ethynyl. In another aspect of this embodiment, R² is F. In another aspect of this embodiment, R² is OH. In another aspect of this embodiment R²² is OH. In another aspect of this embodiment, each R² and R²² is OH. In another aspect of this embodiment, each R² is F and R²² is OH. In another aspect of this embodiment, R⁷ is H. In another aspect of this embodiment, R⁷ is optionally substituted (Ci-Cg)alkyl. In another aspect of this embodiment, R⁷ is optionally substituted (C2-C₈)alkynyl. In another aspect of this embodiment, R⁷ is CN. In another aspect of this embodiment, one of R^c or R^d is H. In another aspect of this embodiment, one of R^c or R^d is H and the other of R^c or R^d is optionally substituted (Ci-Cg)alkyl. In another aspect of this embodiment, R⁶ is optionally substituted (Ci-Cg)alkyl. In another aspect of this embodiment, R⁴ is optionally substituted (Q-Cbojaryl. In another aspect of this embodiment, Ar is optionally substituted nitrophenyl.

In another embodiment of the method for preparing a compound of Formula Xia or Formula Xlb from a compound of Formula XII, X^! is CF, R* is H or CH3 and one of R^c or R^d is H. In another aspect of this embodiment, R² is F. In another aspect of this embodiment, R² is OH. In another aspect of this embodiment R²² is OH. In another aspect of this embodiment, each R² and R²² is OH. In another aspect of this embodiment, each R is F and R is OH. In another aspect of this embodiment, R is H. In another aspect of this embodiment, R⁷ is optionally substituted (CiCs)alkyl. In another aspect of this embodiment, R is CH₃. In another aspect of this embodiment, R⁷ is optionally substituted (Q-Cgjalkynyl. In another aspect of this embodiment, R⁷ is ethynyl. In another aspect of this embodiment, R⁷ is CN. In another aspect of this embodiment, one of R^c or R^d is H and the other of R^c or R^d is optionally substituted (Ci-Cg)alkyl. In another aspect of this embodiment, R⁶ is optionally substituted (CfCsjalkyl. In another aspect of this embodiment, R⁴ is optionally substituted (C6-C2o)aryl. In another aspect of this embodiment, Ar is optionally substituted nitrophenyl. In another aspect of this embodiment, R⁸ is NR^{1 !}R^!2. In another aspect of this embodiment, R⁸ is OR¹ In another aspect of this embodiment, R is NH2. In another aspect of this embodiment, R is OH.

In another embodiment of the method for preparing a compound of Formula XHIaor Formula XIHb from a compound of Formula XIV, X¹ is CF, R¹ is H or CH₃, one of R^c or R^d is H and R⁷ is H. In another aspect of this embodiment, R² is F. In v/ another aspect of this embodiment, R² is OH. In another aspect of this embodiment R²² is OH. In another aspect of this embodiment, each R² and R²² is OH. In another aspect of this embodiment, each R² is F and R²² is OH. In another aspect of this embodiment, one of R^c or R^d is H and the other of R^c or R^d is optionally substituted (Ci-Csjalkyl. In another aspect of this embodiment, one of R^c or R^d is H and the other of R^c or R^d is CH₃. In another aspect of this embodiment, R⁶ is optionally substituted (Ci-Cg)alkyl. In another aspect of this embodiment, R⁴ is optionally substituted (C6-C2o)aryl. In another aspect of this embodiment, R⁴ is optionally substituted phenyl. In another aspect of this embodiment, Ar is optionally substituted nitrophenyl. In another aspect of this embodiment, R⁸ is NR¹ 'R¹². In another aspect of this embodiment, R⁸ is OR¹¹. In another aspect of this embodiment, R⁸ is NH.>. In another aspect of this embodiment, R⁸ is OH.

DEFINITIONS

Unless stated otherwise, the following terms and phrases as used herein are intended to have the following meanings;

When trade names are used herein, applicants intend to independently include the tradename product and the active pharmaceutical ingredient(s) of the tradename product.

As used herein, a compound of the invention or a compound of Formula I means a compound of Formula I or a pharmaceutically acceptable salt, thereof. Similarly, with respect to isolatable intermediates, the phrase a compound of Formula (number) means a compound of that formula and pharmaceutically acceptable salts, thereof.

“Alkyl” is hydrocarbon containing normal, secondary, tertiary or cyclic carbon atoms. For example, an alkyl group can have 1 to 20 carbon atoms (i.e, C]-C₂o alkyl), 1 to 8 carbon atoms (i.e., C|-Cg alkyl), or 1 to 6 carbon atoms (i.e., C.-Q alkyl). Examples of suitable alkyl groups include, but are not limited to, methyl (Me, -CH₃), ethyl (Et, -CH2CH₃), 1-propyl (n-Pr, n-propyl, -CH2CH2CH₃), 2-propyl (i-Pr, i-propyl, -CH(CH₃)2), 1-butyl (n-Bu, n-butyl, -CH2CH2CH2CH₃), 2-methyl-l -propyl (i-Bu, ibutyl, -CH₂CH(CH₃)₂), 2-butyl (s-Bu, s-butyl, -CH(CH₃)CH₂CH₃), 2-methyl-2-propyl (t-Bu, t-butyl, -C(CH3)3), I-pentyl (n-pentyl, -CH₂CH₂CH₂CH₂CH₃), 2-pentyl (-CH(CH3)CH₂CH₂CH3), 3-pentyl (-CH(CH2CH₃)₂), 2-methyl-2-butyl (-C(CH3)2CH₂CH3), 3-methyl-2-butyl (-CH(CH₃)CH(CH3)₂), 3-methyl-l-butyl (-CH2CH2CH(CH₃)₂), 2-methyl-l-butyl (-CH₂CH(CH3)CH₂CH₃), I-hexyl (-CH₂CH₂CH₂CH₂CH₂CH3), 2-hexyl (-CH(CH₃)CH₂CH₂CH₂CH₃), 3-hexyl (CH(CH₂CH3)(CH₂CH₂CH₃)), 2-methyl-2-pentyl (-C(CH₃)2CH₂CH₂CH3), 3-methyl-2pentyl (-CH(CH₃)CH(CH3)CH₂CH3), 4-methyl-2-pentyl (-CH(CH₃)CH₂CH(CH₃)₂), 3-methyl-3-pentyl (-C(CH₂)(C[I₂CHj)i’h 2-methyl-3 -pentyl (CHtCHzCHsjCHtCHsh), 2,3-dimethyl-2-butyl (-C(CH₃)₂CH(CH3)₂), 3,3-dimethyl-2butyl (-CH(CH₃)C(CH3)₃, and octyl (-(CH₂)₇CH₃).

“Alkoxy” means a group having the formula -O-alkyl, in which an alkyl group, as defined above, is attached to the parent molecule via an oxygen atom. The alkyl portion of an alkoxy group can have 1 to 20 carbon atoms (i.e., Ci-C₂o alkoxy), 1 to 12 carbon atoms(i.e., Ci-C]₂ alkoxy), or 1 to 6 carbon atoms(z'.e., Ci-C* alkoxy). Examples of suitable alkoxy groups include, but are not limited to, methoxy (-O-CH3 or-OMe), ethoxy (-OCH2CH3 or -OEt), t-butoxy (-O-C(CH3)3 or -OtBu) and the like.

“Haloalkyl” is an alkyl group, as defined above, in which one or more hydrogen atoms of the alkyl group is replaced with a halogen atom. The alkyl portion of a haloalkyl group can have 1 to 20 carbon atoms (i.e., C]-C₂o haloalkyl), 1 to 12 carbon atoms(Z.e., Ci-C]₂ haloalkyl), or 1 to 6 carbon atoms(i,e., C|-(y, alkyl). Examples of suitable haloalkyl groups include, but are not limited to, -CF₃, -CHF₂, -CFH₂, -CH₂CF₃, and the like.

“Alkenyl” is a hydrocarbon containing normal, secondary, tertiary or cyclic carbon atoms with at least one site of unsaturation, i.e. a carbon-carbon, sp² double bond. For example, an alkenyl group can have 2 to 20 carbon atoms (i.e., C₂-C₂₀alkenyl), 2 to 8 carbon atoms (i.e., C₂-C₈ alkenyl), or 2 to 6 carbon atoms (i.e., C₂-Cy alkenyl). Examples of suitable alkenyl groups include, but are not limited to, ethylene or vinyl (-CH-CH₂), allyl (-CH₂CH=CH₂), cyclopentenyl (-C5H7), and 5-hexenyl (-CH2CH₃CHX'H;CH--CH₂).

“Alkynyl” is a hydrocarbon containing normal, secondary, tertiary or cyclic carbon atoms with at least one site of unsaturation, i.e. a carbon-carbon, sp triple bond. For example, an alkynyl group can have 2 to 20 carbon atoms {i.e., C₂-C₂o alkynyl), 2 to 8 carbon atoms {i.e., C₂-C\ alkyne,), or 2 to 6 carbon atoms {i.e., C₂-Cô alkynyl). Examples of suitable alkynyl groups include, but are not limited to, acetylenic (-C=CH), propargyl (-CH₂C=CH), and the like.

“Alkylene” refers to a saturated, branched or straight chain or cyclic hydrocarbon radical having two monovalent radical centers derived by the removal of two hydrogen atoms from the same or two different carbon atoms of a parent alkane. For example, an alkylene group can have I to 20 carbon atoms, I to IO carbon atoms, or 1 to 6 carbon atoms. Typical alkylene radicals include, but are not limited to, methylene (-CH₂-), 1,1-ethyl (-CH(CH₃)-), 1,2-ethyl (-CH2CH2-), 1,1-propyl (-CH(CH₂CH₃)-), 1,2-propyl (-CH2CH(CH₃)-), 1,3-propyl (-CH2CH2CH2-), 1,4-butyl (-CH₂CH₂CH₂CH₂-), and the like.

“Alkenylene” refers to an unsaturated, branched or straight chain or cyclic hydrocarbon radical having two monovalent radical centers derived by the removal of two hydrogen atoms from the same or two different carbon atoms of a parent alkene. For example, and alkenylene group can have 1 to 20 carbon atoms, 1 to 10 carbon atoms, or 1 to 6 carbon atoms. Typical alkenylene radicals include, but are not limited to, 1,2ethylene (-CH=CH-).

“Alkynylene” refers to an unsaturated, branched or straight chain or cyclic hydrocarbon radical having two monovalent radical centers derived by the removal of two hydrogen atoms from the same or two different carbon atoms of a parent alkyne. For example, an alkynylene group can have 1 to 20 carbon atoms, 1 to 10 carbon atoms, or 1 to 6 carbon atoms. Typical alkynylene radicals include, but are not limited to, acetylene (-C=C-), propargyl (-CH₂Cs=C-), and 4-pentynyl (-CH₂CH₂CH₂C^C-).

“Amino” refers generally to a nitrogen radical which can be considered a derivative of ammonia, having the formula -N(X)₂, where each “X” is independently H, substituted or unsubstituted alkyl, substituted or unsubstituted carbocyclyl, substituted or unsubstituted heterocyclyl, etc. The hybridization of the nitrogen is approximately sp³. Nonlimiting types of amino include -NH₂, -N(alkyl)2, -NH(alkyl), -N(carbocyclyl)₂, - *2

NH(carbocyclyi), -N(heterocyclyl)₂, -NH(heterocyclyl), -N(aryi)2, -NH(aryl), N(alkyl)(aryl), -N(alkyl)(heterocyclyl), -N(carbocyclyl)(heterocyclyl), N(aryl)(heteroaryl), -N(alkyl)(heteroaryl), etc. The term “alkylamino” refers to an amino group substituted with at least one alkyl group. Nonlimiting examples of amino groups include -NH_2j -NH(CH₃), -N(CIL}₂, -NH(CH₂CH₃), - N(CH₂CH₃)₂, NH(phenyl), -N(phenyl)₂, -NH(benzyl), -N(benzyl)₂, etc. Substituted alkylamino refers generally to alkylamino groups, as defined above, in which at least one substituted alkyl, as defined herein, is attached to the amino nitrogen atom. Non-limiting examples of substituted alkylamino includes -NH(alkylene-C(O)-OH), -NH(alkylene-C(O)-O-alkyl), -N(alkylene-C(O)-OH)₂, -N(alkylene-C(0)-O-alkyl)₂, etc.

“Aryl” means an aromatic hydrocarbon radical derived by the removal of one hydrogen atom from a single carbon atom of a parent aromatic ring system. For example, an aryl group can have 6 to 20 carbon atoms, 6 to 14 carbon atoms, or 6 to 10 carbon atoms. Typical aryl groups include, but are not limited to, radicals derived from benzene (e.g., phenyl), substituted benzene, naphthalene, anthracene, biphenyl, and the like.

“Arylalkyl” refers to an acyclic alkyl radical in which one of the hydrogen atoms bonded to a carbon atom, typically a terminal or sp³ carbon atom, is replaced with an aryl radical. Typical arylalkyl groups include, but are not limited to, benzyl, 2-phenylethan-l-yl, naphthylmethyl, 2-naphthylethan-l-yl, naphthobenzyl, 2-naphthophenylethan-l-yl and the like. The aryl alkyl group can comprise 7 to 20 carbon atoms, e.g., the alkyl moiety is 1 to 6 carbon atoms and the aryl moiety is 6 to 14 carbon atoms.

“Arylalkenyl” refers to an acyclic alkenyl radical in which one of the hydrogen atoms bonded to a carbon atom, typically a terminal or sp³ carbon atom, but also an sp² carbon atom, is replaced with an aryl radical. The aryl portion of the arylalkenyl can include, for example, any of the aryl groups disclosed herein, and the alkenyl portion of the arylalkenyl can include, for example, any of the alkenyl groups disclosed herein. The arylalkenyl group can comprise 8 to 20 carbon atoms, e.g., the alkenyl moiety is 2 to 6 carbon atoms and the aryl moiety is 6 to 14 carbon atoms.

“Arylalkynyl” refers to an acyclic alkynyl radical in which one of the hydrogen atoms bonded to a carbon atom, typically a terminal or sp carbon atom, but also an sp carbon atom, is replaced with an aryl radical. The aryl portion of the arylalkynyl can include, for example, any of the aryl groups disclosed herein, and the alkynyl portion of the arylalkynyl can include, for example, any of the alkynyl groups disclosed herein. The arylalkynyl group can comprise 8 to 20 carbon atoms, e.g., the alkynyl moiety is 2 to 6 carbon atoms and the aryl moiety is 6 to 14 carbon atoms.

The term “substituted” in reference to alkyl, alkylene, aryl, arylalkyl, alkoxy, heterocyclyl, heteroaryl, carbocyclyl, etc., for example, “substituted alkyl”, “substituted alkylene”, “substituted aryl”, “substituted arylalkyl”, “substituted heterocyclyl”, and “substituted carbocyclyl” means, unless otherwise stated, alkyl, alkylene, aryl, arylalkyl, heterocyclyl, carbocyclyl respectively, in which one or more hydrogen atoms are each independently replaced with a non-hydrogen substituent. Typical substituents include, but are not limited to, -X, -R^b, -O', =O, -OR^b, -SR^b, -S', -NR^b2, -N⁺R^b3, =NR^b, -CX3, -CN, -OCN, -SCN, -N- C 0. -NCS, -NO, -NO2, =N2, -N3, -NHC(=O)R^b, -OC(=O)R^b, -NHC(=O)NR^b2, -S(=O)₂-, -S(O)₂OH. -S(=O)₂R^b, -OS(=O)2OR^b, -S(=O)2NR^b2, -S(=O)R^b, -OP(=O)(OR^b)2,-P(=O)(OR^b)2, -P(=O)(O')2, -P(=O)(OH)2, -P(O)(OR^b)(O ), -C(=O)R^b, -C(=O)X, -C(S)R^b, -C(O)OR^b, -C(O)O', -C(S)OR^b, -C(O)SR^b, -C(S)SR^b, -C(O)NR^b2, -C{S)NR^b2, -C(=NR^b)NR^b2, where each X is independently a halogen: F, Cl, Br, or I; and each R^b is independently H, alkyl, aryl, arylalkyl, a heterocycle, or a protecting group or prodrug moiety. Alkylene, alkenylene, and alkynylene groups may also be similarly substituted. Unless otherwise indicated, when the term substituted is used in conjunction with groups such as arylalkyl, which have two or more moieties capable of substitution, the substituents can be attached to the aryl moiety, the alkyl moiety, or both.

The term “prodrug” as used herein refers to any compound that when administered to a biological system generates the drug substance, i.e., active ingredient, as a result of spontaneous chemical reactions), enzyme catalyzed chemical reaction^), photolysis, and/or metabolic chemical reaction(s). A prodrug is thus a covalently modified analog or latent form of a therapeutically active compound.

One skilled in the art will recognize that substituents and other moieties of the compounds of Formula I-XIV should be selected in order to provide a compound which is sufficiently stable to provide a pharmaceutically useful compound which can be formulated into an acceptably stable pharmaceutical composition. Compounds of Formula I-XIV which have such stability are contemplated as falling within the scope of the present invention.

“Heteroalkyl” refers to an alkyl group where one or more carbon atoms have been replaced with a heteroatom, such as, Ο, N, or S. For example, if the carbon atom of the alkyl group which is attached to the parent molecule is replaced with a heteroatom (e.g., Ο, N, or S) the resulting heteroalkyl groups are, respectively, an alkoxy group (e.g., -OCH3, etc.), an amine (e.g., -NHCH3, -N(CI L)₂, etc.), or a thioalkyl group (e.g., -SCH3). If a non-terminal carbon atom of the alkyl group which is not attached to the parent molecule is replaced with a heteroatom (e.g., Ο, N, or S) the resulting heteroalkyl groups are, respectively, an alkyl ether (e.g., -CH2CH2-O-CH3, etc.), an alkyl amine (e g., -CH2NHCH3, -CH2N(CH₃)2, etc.), or a thioalkyl ether (e.g.,-CH2-S-CH3). If a terminal carbon atom of the alky) group is replaced with a heteroatom (e.g., Ο, N, or S), the resulting heteroalkyl groups are, respectively, a hydroxyalkyl group (e.g., -CH₂CH₂-OH), an aminoalkyl group (e.g., -CH2NH2), or an alkyl thiol group (e.g., -CH₂CH₂-SH). A heteroalkyl group can have, for example, 1 to 20 carbon atoms, 1 to 10 carbon atoms, or 1 to 6 carbon atoms. A ()-(), heteroalkyl group means a heteroalkyl group having 1 to 6 carbon atoms.

“Heterocycle” or “heterocyclyl” as used herein includes by way of example and not limitation those heterocycles described in Paquette, Leo A.; Principles of Modem Heterocyclic Chemistry (W.A. Benjamin, New York, 1968), particularly Chapters I, 3,4, 6, 7, and 9; The Chemistry of Heterocyclic Compounds, A Series of Monographs” (John Wiley & Sons, New York, 1950 to present), in particular Volumes 13, 14, 16, 19, and 28; and J. Am. Chem. Soc. (1960) 82:5566. In one specific embodiment of the invention “heterocycle” includes a “carbocycle” as defined herein, wherein one or more (e.g. 1, 2, 3, or 4) carbon atoms have been replaced with a heteroatom (e.g. Ο, N, or S). The terms “heterocycle” or “heterocyclyl” includes saturated rings, partially unsaturated rings, and aromatic rings (i.e., heteroaromatic rings). Substituted heterocyclyls include, for example, heterocyclic rings substituted with any of the substituents disclosed herein including carbonyl groups. A non-limiting example of a carbonyl substituted heterocyclyl is:

Examples of heterocycles include by way of example and not limitation pyridyl, dihydroypyridyl, tetrahydropyridyl (piperidyl), thiazolyl, tetrahydrothiophenyl, sulfur oxidized tetrahydrothiophenyl, pyrimidinyl, furanyl, thienyl, pyrrolyl, pyrazolyl, imidazolyl, tetrazolyl, benzo furanyl, thianaphthalenyl, indolyl, indolenyl, quinolinyl, isoquinolinyl, benzimidazolyl, piperidinyl, 4piperidonyl, pyrrolidinyl, 2-pyrrolidonyl, pyrrolinyl, tetrahydro furanyl, tetrahydroquinolinyl, tetrahydroisoquinolinyl, decahydroquinolinyl, octahydroisoquinolinyl, azocinyl, triazinyl, 6H-l,2,5-thiadiazinyl, 2H,6H-l,5,2dithiazinyl, thienyl, thianthrenyl, pyranyl, isobenzofuranyl, chromenyl, xanthenyl, phenoxathinyl, 2H-pyrrolyl, isothiazolyl, isoxazolyl, pyrazinyl, pyridazinyl, indolizinyl, isoindolyl, 3H-indolyl, lH-indazoly, purinyl, 4H-quinolizinyl, phthalazinyl, naphthyridinyl, quinoxalinyl, quinazolinyl, cinnolinyl, pteridinyl, 4aHcarbazolyl, carbazolyl, β-carbolinyl, phenanthridinyl, acridinyl, pyrimidinyl, phenanthrolinyl, phenazinyl, phenothiazinyl, furazanyl, phenoxazinyl, isochromanyl, chromanyl, imidazolidinyl, imidazolinyl, pyrazolidinyl, pyrazolinyl, piperazinyl, indolinyl, isoindolinyl, quinuclidinyl, morpholinyl, oxazolidinyl, benzotriazolyl, benzisoxazolyl, oxindolyl, benzoxazolinyl, isatinoyl, and bis-tetrahydro furanyl:

By way of example and not limitation, carbon bonded heterocycles are bonded at position 2, 3, 4, 5, or 6 of a pyridine, position 3, 4, 5, or 6 of a pyridazine, position

2, 4, 5, or 6 of a pyrimidine, position 2, 3, 5, or 6 of a pyrazine, position 2, 3,4, or 5 of a furan, tetrahydrofuran, thiofuran, thiophene, pyrrole or tetrahydropyrrole, position 2, 4, or 5 of an oxazole, imidazole or thiazole, position 3, 4, or 5 of an isoxazole, pyrazole, or isothiazole, position 2 or 3 of an aziridine, position 2, 3, or 4 of an azetidine, position 2, 3,4, 5, 6, 7, or 8 of a quinoline or position 1, 3,4, 5, 6, 7, or 8 of an isoquinoline. Still more typically, carbon bonded heterocycles include 2pyridyl, 3-pyridyl, 4-pyridyl, 5-pyridyl, 6-pyridyl, 3-pyridazinyl, 4-pyridazinyl, 5pyridazinyl, 6-pyridazinyl, 2-pyrimidinyl, 4-pyrimidinyl, 5-pyrimidinyl, 6pyrimidinyl, 2-pyrazinyl, 3-pyrazinyl, 5-pyrazinyl, 6-pyrazinyl, 2-thiazolyl, 4thiazolyl, or 5-thiazolyl.

By way of example and not limitation, nitrogen bonded heterocycles are bonded at position 1 of an aziridine, azetidine, pyrrole, pyrrolidine, 2-pyrroline, 3pyrroline, imidazole, imidazolidine, 2-imidazoline, 3-imidazoline, pyrazole, pyrazoline, 2-pyrazoline, 3-pyrazoline, piperidine, piperazine, indole, indoline, 1Hindazole, position 2 of a isoindole, or isoindoline, position 4 of a morpholine, and position 9 of a carbazole, or β-carboline. Still more typically, nitrogen bonded heterocycles include 1-aziridyl, 1-azetedyl, 1-pyrrolyl, 1-imidazolyl, 1-pyrazolyl, and 1-piperidinyl.

“Heterocyclylalkyl” refers to an acyclic alkyl radical in which one of the hydrogen atoms bonded to a carbon atom, typically a terminal or sp carbon atom, is replaced with a heterocyclyl radical (i.e., a heterocyclyl-alkylene- moiety). Typical heterocyclyl alkyl groups include, but are not limited to heterocyclyl-CH2-, 2(heterocyclyl)ethan-l-yl, and the like, wherein the “heterocyclyl” portion includes any of the heterocyclyl groups described above, including those described in Principles of Modem Heterocyclic Chemistry. One skilled in the art will also understand that the heterocyclyl group can be attached to the alkyl portion of the heterocyclyl alkyl by means of a carbon-carbon bond or a carbon-heteroatom bond, with the proviso that the resulting group is chemically stable. The heterocyclyl alkyl group comprises 3 to 20 carbon atoms, e.g., the alkyl portion of the arylalkyl group is 1 to 6 carbon atoms and the heterocyclyl moiety is 2 to 14 carbon atoms. Examples of heterocyclylalkyls include by way of example and not limitation 5-membered sulfur, oxygen, and/or nitrogen containing heterocycles such as thiazolylmethyl, 2-thiazolylethan-1 -yl, imidazolylmethyl, oxazolylmethyl, thiadiazolylmethyl, etc., 6-membered sulfur, oxygen, and/or nitrogen containing heterocycles such as piperidinylmethyl, piperazinylmethyl, morpholinylmethyl, pyridinylmethyl, pyridizylmethyl, pyrimidylmethyl, pyrazinylmethyl, etc.

“Heterocyclylalkenyl” refers to an acyclic alkenyl radical in which one of the hydrogen atoms bonded to a carbon atom, typically a terminal or sp carbon atom, but also a sp² carbon atom, is replaced with a heterocyclyl radical (i.e., a heterocyclyl alkenylene- moiety). The heterocyclyl portion of the heterocyclyl alkenyl group includes any of the heterocyclyl groups described herein, including those described in Principles of Modem Heterocyclic Chemistry, and the alkenyl portion of the heterocyclyl alkenyl group includes any of the alkenyl groups disclosed herein. One skilled in the art will also understand that the heterocyclyl group can be attached to the alkenyl portion of the heterocyclyl alkenyl by means of a carbon-carbon bond or a carbon-heteroatom bond, with the proviso that the resulting group is chemically stable. The heterocyclyl alkenyl group comprises 4 to 20 carbon atoms, e.g., the alkenyl portion of the heterocyclyl alkenyl group is 2 to 6 carbon atoms and the heterocyclyl moiety is 2 to 14 carbon atoms.

“Heterocyclylalkynyl” refers to an acyclic alkynyl radical in which one of the

J hydrogen atoms bonded to a carbon atom, typically a terminal or sp carbon atom, but also an sp carbon atom, is replaced with a heterocyclyl radical (i.e., a heterocyclylalkynylene- moiety). The heterocyclyl portion of the heterocyclyl alkynyl group includes any of the heterocyclyl groups described herein, including those described in Principles of Modem Heterocyclic Chemistry, and the alkynyl portion of the heterocyclyl alkynyl group includes any of the alkynyl groups disclosed herein. One skilled in the art will also understand that the heterocyclyl group can be attached to the alkynyl portion of the heterocyclyl alkynyl by means of a carbon-carbon bond or a carbon-heteroatom bond, with the proviso that the resulting group is chemically stable. The heterocyclyl alkynyl group comprises 4 to 20 carbon atoms, e.g., the alkynyl portion of the heterocyclyl alkynyl group is 2 to 6 carbon atoms and the heterocyclyl moiety is 2 to 14 carbon atoms.

“Heteroaryl” refers to an aromatic heterocyclyl having at least one heteroatom in the ring. Non-limiting examples of suitable heteroatoms which can be included in <λ/ the aromatic ring include oxygen, sulfur, and nitrogen. Non-limiting examples of heteroaryl rings include all of those aromatic rings listed in the definition of “heterocyclyl”, including pyridinyl, pyrrolyl, oxazolyl, indolyl, isoindolyl, purinyl, furanyl, thienyl, benzo furanyl, benzothiophenyl, carbazolyl, imidazolyl, thiazolyl, isoxazolyl, pyrazolyl, isothiazolyl, quinolyl, isoquinolyl, pyridazyl, pyrimidyl, pyrazyl, etc.

The term “purine” or “pyrimidine” base comprises, but is not limited to, adenine, N⁶-alkylpurines, N⁶~acylpurines (wherein acyl is C(O)(alkyl, aryl, alkylaryl, or arylalkyl), N⁶-benzylpurine, N⁶-halopurine, N⁶-vinylpurine, N⁶-acetylenic purine, N⁶-acyl purine, N⁶-hydroxyalkyl purine, N⁶-aIlylaminopurine, N⁶-thioallyl purine, N²alkylpurines, N²-alkyl-6-thiopurines, thymine, cytosine, 5-fluorocytosine, 5methylcytosine, 6-azapyrimidine, including 6-azacytosine, 2- and/or 4mercaptopyrmidine, uracil, 5-halouracil, including 5-fluorouracil, C^salkylpyrimidines, C⁵-benzylpyrimidines, C⁵-halopyrimîdines, C^s-vinylpyrimidîne, C⁵acetylenic pyrimidine, C⁵-acyl pyrimidine, C^s-hydroxyalkyl purine, C^samidopyrimidine, C⁵-cyanopyrimidine, C⁵-5-iodopyrimidine, C⁶-iodo-pyrimidine, C⁵Br-vinyl pyrimidine, C⁶-Br-vinyl pyrimidine, C⁵-nitropyrimidine, C⁵-aminopyrimidine, N²-alkyIpurines, N²-alkyl-6-thiopurines, 5-azacytidinyl, 5-azauracilyl, triazolopyridinyl, imidazolopyridinyl, pyrrolopyrimidinyl, and pyrazolopyrimidinyl. Purine bases include, but are not limited to, guanine, adenine, hypoxanthine, 2,6diaminopurine, and 6-chloropurine. Additional non-classical purine bases include pyrrolof 1,2-f][1,2,4]triazines, imidazof 1,5-f)[ 1,2,4]triazines, imidazof 1,2f][l,2,4]triazines, and [l,2,4]triazolo[4,3-f][l,2,4]triazines, all of which are optionally substituted. The purine and pyrimidine bases of Formula II are linked to the ribose sugar, or analog thereof, through a nitrogen atom or carbon atom of the base. Functional oxygen and nitrogen groups on the base can be protected as necessary or desired. Suitable protecting groups are well known to those skilled in the art, and include, but are not limited to, trimethylsilyl, dimethylhexylsilyl, t-butyldimethylsilyl, and t-butyldiphenylsilyl, trityl, alkyl groups, and acyl groups such as acetyl and propionyl, methanesulfonyl, and p-toluenesulfonyl.

“Carbocycle” or “carbocyclyl” refers to a saturated (i.e., cycloalkyl), partially unsaturated (e.g., cycloakenyl, cycloalkadienyl, etc.) or aromatic ring having 3 to 7 carbon atoms as a monocycle, 7 to 12 carbon atoms as a bicycle, and up to about 20 carbon atoms as a polycycle. Monocyclic carbocycles have 3 to 7 ring atoms, still more typically 5 or 6 ring atoms. Bicyclic carbocycles have 7 to 12 ring atoms, e.g., arranged as a bicyclo [4,5], [5,5], [5,6] or [6,6] system, or 9 or 10 ring atoms arranged as a bicyclo [5,6] or [6,6] system, or spiro-fused rings. Non-limiting examples of monocyclic carbocycles include cyclopropyl, cyclobutyl, cyclopentyl, 1-cyclopent-1enyl, l-cyclopent-2-enyl, 1-cyclopent-3-enyl, cyclohexyl, 1-cyclohex-1-enyl, 1cyclohex-2-enyl, l-cyclohex-3-enyl, and phenyl. Non-limiting examples of bicyclo carbocycles includes naphthyl, tetrahydronapthalene, and decaline.

“Carbocyclylalkyl” refers to to an acyclic alkyl radical in which one of the hydrogen atoms bonded to a carbon atom is replaced with a carbocyclyl radical as described herein. Typical, but non-limiting, examples of carbocyclylalkyl groups include cyclopropylmethyl, cyclopropylethyl, cyclobutylmethyl, cyclopentylmethyl and cyclohexylmethyl.

“Arylheteroalkyl” refers to a heteroalkyl as defined herein, in which a hydrogen atom (which may be attached either to a carbon atom or a heteroatom) has been replaced with an aryl group as defined herein. The aryl groups may be bonded to a carbon atom of the heteroalkyl group, or to a heleroatom of the heteroalkyl group, provided that the resulting arylheteroalkyl group provides a chemically stable moiety. For example, an arylheteroalkyl group can have the general formulae -alkyleneO-aryl, -alkylene-O-alkylene-aryl, -alkylene-NH-aryl, -alkylene-NH-alkylene-aryl, -alkylene-S-aryl, -alkyl ene-S-alkylene-aryl, etc. In addition, any of the alkylene moieties in the general formulae above can be further substituted with any of the substituents defined or exemplified herein.

“Heteroarylalkyl” refers to an alkyl group, as defined herein, in which a hydrogen atom has been replaced with a heteroaryl group as defined herein. Nonlimiting examples of heteroaryl alkyl include -CH₂-pyridinyl, -CH₂-pyrrolyl, -CH₂-oxazolyl, -CH₂-indolyl, -CH₂-isoindolyl, -CH₂-purinyl, -CH₂-furanyl, -CH₂-thienyl, -CH₂-benzofuranyl, -CH₂-benzothiophenyl, -CH₂-carbazolyl, -CH₂-imidazolyl, -CH₂-thiazolyl, -CH₂-isoxazolyl, -CH₂-pyrazolyl, -CH₂-isothiazolyl, vt/

-CH₂-quinolyI, -CIh-isoquinolyl, -CH₂-pyridazyl, -Clb-pyrimidyl, -CI L-pyrazyh -CH(CH₃)-pyridinyl, -CH(CH₃)-pyrrolyl, -CH(CH₃)-oxazolyl, -CH(CH₃)-indolyl, -CH(CH₃)-isoindolyl, -CH(CH₃)-purinyl, -CH(CH₃)-furanyl, -CH(CH₃)-thienyl, -CH(CH₃)-benzofuranyI, -CH(CH₃)-benzothiophenyl, -CH(CH₃)-carbazolyl, -CH(CH₃)-imidazolyl, -CH(CH₃)-thiazolyl, -CH(CH₃)-isoxazolyl, -CH(CH₃)-pyrazolyl, -CH(CH₃)-isothiazolyl, -CH(CH₃)-quinolyl, -CH(CH₃)-isoquinolyl, -CH(CH₃)-pyridazyl, -CH(CH₃)-pyrimidyl, -CH(CH₃)-pyrazyl, etc.

The term “optionally substituted” in reference to a particular moiety of the compound of Formula I-XIV (e.g., an optionally substituted aryl group) refers to a moiety wherein all substiutents are hydrogen or wherein one or more of the hydrogens of the moiety may be replaced by substituents such as those listed under the definition of “substituted”.

The term “optionally replaced” in reference to a particular moiety of the compound of Formula I-XIV (e.g., the carbon atoms of said (Ci-Cs)alkyl may be optionally replaced by -0-, -S-, or -NR^a-) means that one or more of the methylene groups of the (Ci-Cg)alkyl may be replaced by 0, 1,2, or more of the groups specified (e.g.,-O-, -S-, or -NR^a-).

The term “non-terminal carbon atom(s)” in reference to an alkyl, alkenyl, alkynyl, alkylene, alkenylene, or alkynylene moiety refers to the carbon atoms in the moiety that intervene between the first carbon atom of the moiety and the last carbon atom in the moiety. Therefore, by way of example and not limitation, in the alkyl moiety -CH₂(C*)H2(C*)H2CH₃ or alkylene moiety -CH₂(C )H₂(C )H2CH2- the C atoms would be considered to be the non-terminal carbon atoms.

Linker or “link” means a chemical moiety comprising a covalent bond or a chain of atoms. Linkers include repeating units of alkyloxy (e.g. polyethyleneoxy, PEG, polymethyleneoxy) and alkylamino (e.g. polyethyleneamino, Jeffamine™); and diacid ester and amides including succinate, succinamide, diglycolate, malonate, and caproamide.

The terms such as “oxygen-linked”, “nitrogen-linked”, “carbon-linked”, “sulfur-linked”, or “phosphorous-linked” mean that if a bond between two moieties v can be formed by using more than one type of atom in a moiety, then the bond formed between the moieties is through the atom specified. For example, a nitrogen-linked amino acid would be bonded through a nitrogen atom of the amino acid rather than through an oxygen or carbon atom of the amino acid.

Some embodiments of the compounds of Formula I-XIV comprise the moiety

which may comprise a radical of a nitrogen-linked ester of a naturally occurring aamino acid. Examples of naturally occurring amino acids include isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan, valine, alanine, asparagine, aspartic acid, cysteine, glutamic acid, glutamine, glycine, proline, selenocysteine, serine, tyrosine, arginine, histidine, ornithine and taurine. The esters of these amino acids comprise any of those described for the substitutent R⁶, particularly those in which R⁶ is optionally substituted (Cj-Cgjalkyl.

Unless otherwise specified, the carbon atoms of the compounds of Formula I15 XIV are intended to have a valence of four. In some chemical structure representations where carbon atoms do not have a sufficient number of variables attached to produce a valence of four, the remaining carbon substitutents needed to provide a valence of four should be assumed to be hydrogen. For example,

“Protecting group” refers to a moiety of a compound that masks or alters the properties of a functional group or the properties of the compound as a whole. The chemical substructure of a protecting group varies widely. One function of a protecting group is to serve as an intermediate in the synthesis of the parental drug substance. Chemical protecting groups and strategies for protection/deprotection are well known in the art. See: Protective Groups in Organic Chemistry, Theodora W. Greene (John Wiley & Sons, Inc., New York, 1991. Protecting groups are often utilized to mask the reactivity of certain functional groups, to assist in the efficiency of desired chemical reactions, e.g. making and breaking chemical bonds in an ordered and planned fashion. Protection of functional groups of a compound alters other physical properties besides the reactivity of the protected functional group, such as the polarity, lipophilicity (hydrophobicity), and other properties which can be measured by common analytical tools. Chemically protected intermediates may themselves be biologically active or inactive.

Protected compounds may also exhibit altered, and in some cases, optimized properties in vitro and in vivo, such as passage through cellular membranes and resistance to enzymatic degradation or sequestration. In this role, protected compounds with intended therapeutic effects may be referred to as prodrugs. Another function of a protecting group is to convert the parental drug into a prodrug, whereby the parental drug is released upon conversion of the prodrug in vivo. Because active prodrugs may be absorbed more effectively than the parental drug, prodrugs may possess greater potency in vivo than the parental drug. Protecting groups are removed either in vitro, in the instance of chemical intermediates, or in vivo, in the case of prodrugs. With chemical intermediates, it is not particularly important that the resulting products after deprotection, e.g. alcohols, be physiologically acceptable, although in general it is more desirable if the products are pharmacologically innocuous.

“Prodrug moiety” means a labile functional group which separates from the active inhibitory compound during metabolism, systemically, inside a cell, by hydrolysis, enzymatic cleavage, or by some other process (Bundgaard, Hans, “Design and Application of Prodrugs” in Textbook of Drug Design and Development (1991), P. Krogsgaard-Larsen and H. Bundgaard, Eds. Harwood Academic Publishers, pp. 113191). Enzymes which are capable of an enzymatic activation mechanism with the phosphonate prodrug compounds of the invention include, but are not limited to, amidases, esterases, microbial enzymes, phospholipases, cholinesterases, and phosphases. Prodrug moieties can serve to enhance solubility, absorption and lipophilicity to optimize drug delivery, bioavailability and efficacy. A prodrug moiety may include an active metabolite or drug itself.

The phosphate group may be a phosphate prodrug moiety. The prodrug moiety may be sensitive to hydrolysis. Alternatively, the prodrug moiety may be sensitive to enzymatic cleavage, such as a lactate ester or a phosphonamidate-ester group.

It is to be noted that all enantiomers, diastereomers, and racemic mixtures, tautomers, polymorphs, pseudopolymorphs of compounds within the scope of Formula I-IV and pharmaceutically acceptable salts thereof are embraced by the present invention. All mixtures of such enantiomers and diastereomers are within the scope of the present invention.

A compound of Formula I-XIV and its pharmaceutically acceptable salts may exist as different polymorphs or pseudopolymorphs. As used herein, crystalline polymorphism means the ability of a crystalline compound to exist in different crystal structures. The crystalline polymorphism may result from differences in crystal packing (packing polymorphism) or differences in packing between different conformers of the same molecule (conformational polymorphism). As used herein, <\ crystalline pseudopolymoiphism means the ability of a hydrate or solvate of a compound to exist in different crystal structures. The pseudopolymorphs of the instant invention may exist due to differences in crystal packing (packing pseudopolymorphism) or due to differences in packing between different conformers of the same molecule (conformational pseudopolymorphism). The instant invention comprises all polymorphs and pseudopolymorphs of the compounds of Formula I-IV and their pharmaceutically acceptable salts.

A compound of Formula I-XIV and its pharmaceutically acceptable salts may also exist as an amorphous solid. As used herein, an amorphous solid is a solid in which there is no long-range order of the positions of the atoms in the solid. This definition applies as well when the crystal size is two nanometers or less. Additives, including solvents, may be used to create the amorphous forms of the instant invention. The instant invention comprises all amorphous forms of the compounds of Formula I-IV and their pharmaceutically acceptable salts.

Selected substituents comprising the compounds of Formula I-XIV are present to a recursive degree. In this context, “recursive substituent” means that a substituent may recite another instance of itself. Because of the recursive nature of such substituents, theoretically, a large number of compounds may be present in any given embodiment. One of ordinary skill in the art of medicinal chemistry understands that the total number of such substituents is reasonably limited by the desired properties of the compound intended. Such properties include, by way of example and not limitation, physical properties such as molecular weight, solubility or log P, application properties such as activity against the intended target, and practical properties such as ease of synthesis. Recursive substituents are an intended aspect of the invention. One of ordinary skill in the art of medicinal chemistry understands the versatility of such substituents. To the degree that recursive substituents are present in an embodiment of the invention, they may recite another instance of themselves, 0, I, 2,3, or 4 times.

The modifier about used in connection with a quantity is inclusive of the stated value and has the meaning dictated by the context (e.g., includes the degree of error associated with measurement of the particular quantity). xh/'

Any reference to the compounds of the invention described heerein also includes a reference to a physiologically acceptable salt thereof. Examples of physiologically acceptable salts of the compounds of the invention include salts derived from an appropriate base, such as an alkali metal or an alkaline earth (for example, Na⁺, Li⁺, K⁺> Ca¹ and Mg¹ ^), ammonium and NR³,/ (wherein R^a is defined herein). Physiologically acceptable salts of a nitrogen atom or an amino group include (a) acid addition salts formed with inorganic acids, for example, hydrochloric acid, hydrobromic acid, sulfuric acid, sulfamic acids, phosphoric acid, nitric acid and the like; (b) salts formed with organic acids such as, for example, acetic acid, oxalic acid, tartaric acid, succinic acid, maleic acid, fumaric acid, gluconic acid, citric acid, malic acid, ascorbic acid, benzoic acid, isethionic acid, lactobionic acid, tannic acid, palmitic acid, alginic acid, polyglutamic acid, naphthalenesulfonic acid, methanesulfonic acid, p-toluenesulfonic acid, benzenesulfonic acid, naphthalenedisulfonic acid, polygalacturonic acid, malonic acid, sulfosalicylic acid, glycolic acid, 2-hydroxy-3-naphthoate, pamoate, salicylic acid, stearic acid, phthalic acid, mandelic acid, lactic acid, ethanesulfonic acid, lysine, arginine, glutamic acid, glycine, serine, threonine, alanine, isoleucine, leucine and the like; and (c) salts formed from elemental anions for example, chlorine, bromine, and iodine. Physiologically acceptable salts of a compound of a hydroxy group include the anion of said compound in combination with a suitable cation such as Na⁺ and NR^a ₄ ⁺.

For therapeutic use, salts of active ingredients of the compounds of the invention will be physiologically acceptable, i.e. they will be salts derived from a physiologically acceptable acid or base. However, salts of acids or bases which are not physiologically acceptable may also find use, for example, in the preparation or purification of a physiologically acceptable compound. All salts, whether or not derived form a physiologically acceptable acid or base, are within the scope of the present invention.

Finally, it is to be understood that the compositions herein comprise compounds of the invention in their un-ionized, as well as zwitterionic form, and combinations with stoichiometric amounts of water as in hydrates.

The compounds of the invention, exemplified by Formula I-XIV have chiral centers, e.g. chiral carbon or phosphorus atoms. For example, the phosphorous atoms of Formula I-XIV may be chiral because they have four different substituents. The compounds of the invention thus include racemic mixtures of all stereoisomers, including enantiomers, diastereomers, and atropisomers. In addition, the compounds of the invention include enriched or resolved optical isomers at any or all asymmetric, chiral atoms. In other words, the chiral centers apparent from the depictions are provided as the chiral isomers or racemic mixtures. Both racemic and diastereomeric mixtures, as well as the individual optical isomers isolated or synthesized, substantially free of their enantiomeric or diastereomeric partners, are all within the scope of the invention. The racemic mixtures are separated into their individual, substantially optically pure isomers through well-known techniques such as, for example, the separation of diastereomeric salts formed with optically active adjuncts, e.g., acids or bases followed by conversion back to the optically active substances. In most instances, the desired optical isomer is synthesized by means of stereospecific reactions, beginning with the appropriate stereoisomer of the desired starting material.

The term chiral refers to molecules which have the property of nonsuperimposability of the mirror image partner, while the term achiral refers to molecules which are superimposable on their mirror image partner.

The term stereoisomers refers to compounds which have identical chemical constitution, but differ with regard to the arrangement of the atoms or groups in space.

Diastereomer refers to a stereoisomer with two or more centers of chirality and whose molecules are not mirror images of one another. Diastereomers have different physical properties, e.g. melting points, boiling points, spectral properties, reactivities and biological properties. For example, the compounds of Formula I-XIV may have a chiral phosphorus atom when phosphorus has four different substitutents, e.g., Formula XIV, where the chirality is R or S. When R^c and R^d of the amino acid of the phosphoramidate of Formula IV are different, there are two centers of chirality in the molecule leading to potential diastereomeric mixtures of compounds, e.g. R.S; 5,Λ; S,S and R,R isomers. Mixtures of diastereomers may be separate under high resolution analytical procedures such as electrophoresis, crystallization and/or chromatography. Diastereomeres may have different physical attributes such as, but not limited to, solubility, chemical stabilities and crystallinity and may also have different biological properties sue as, but not limited to, enzymatic stability, absorption and metabolic stability.

Enantiomers refer to two stereoisomers of a compound which are nonsuperimposable mirror images of one another.

Stereochemical definitions and conventions used herein generally follow S. P. Parker, Ed., McGraw-Hill Dictionary of Chemical Terms (1984) McGraw-Hill Book Company, New York; and Eliel, E. and Wilen, S., Stereochemistry of Organic Compounds (1994) John Wiley & Sons, Inc., New York. Many organic compounds exist in optically active forms, i.e., they have the ability to rotate the plane of planepolarized light. In describing an optically active compound, the prefixes D and L or R and S are used to denote the absolute configuration of the molecule about its chiral center(s). The prefixes d and 1, D and L, or (+) and (-) are employed to designate the sign of rotation of plane-polarized light by the compound, with S, (-), or 1 meaning that the compound is levorotatory while a compound prefixed with R, (+), or d is dextrorotatory. For a given chemical structure, these stereoisomers are identical except that they are mirror images of one another. A specific stereoisomer may also be referred to as an enantiomer, and a mixture of such isomers is often called an enantiomeric mixture. A 50:50 mixture of enantiomers is referred to as a racemic mixture or a racemate, which may occur where there has been no stereoselection or stereospecificity in a chemical reaction or process. The terms racemic mixture and racemate refer to an equimolar mixture of two enantiomeric species, devoid of optical activity.

Whenever a compound described herein is substituted with more than one of the same designated group, e.g., R^a or R¹, then it will be understood that the groups may be the same or different, i.e., each group is independently selected. Wavy lines, , indicate the site of covalent bond attachments to the adjoining substructures, groups, moieties, or atoms.

The compounds of the invention can also exist as tautomeric isomers in certain cases. Although only one delocalized resonance structure may be depicted, all such njJ forms are contemplated within the scope of the invention. For example, ene-amine tautomers can exist for purine, pyrimidine, imidazole, guanidine, amidine, and tetrazole systems and all their possible tautomeric forms are within the scope of the invention.

One skilled in the art will recognize that nucleoside bases such as the pyrrolof l,2-f][I,2,4]triazine nucleosides can exist in tautomeric forms. For example, but not by way of limitation, structures (a) and (b) can have equivalent tautomeric forms as shown below:

IO

All possible tautomeric forms of the heterocycles and nucleobases in all of the embodiments disclosed herein are within the scope of the invention.

The compounds of Formula I-XIV also include molecules that incorporate isotopes of the atoms specified in the particular molecules. Non-limiting examples of 15 these isotopes include D, T, ^!4C, ^l3C and ^l5N. All such isotopic variations of these molelcules are provided by the instant invention.

Examples

Certain abbreviations and acronyms are used in describing the experimental details. Although most of these would be understood by one skilled in the art, Table I 5 contains a list of many of these abbreviations and acronyms.

Table 1. List of abbreviations and acronyms.

Abbreviation	Meaning
Ac₂O	acetic anhydride
AIBN	2,2’-azobis(2-methylpropionitrile)
Bn	benzyl
BnBr	benzylbromide
BSA	bis(trimethylsilyl)acetamide
BzCl	benzoyl chloride
CD1	carbonyl diimidazole
DABCO	l,4-diazabicyclo[2.2.2]octane
DBN	1,5-diazabicyclo[4.3.0]nonene-5
DDQ	2,3-dichloro-5,6-dicyano-l,4-benzoquinone
DBU	l,5-diazabicyclo[5.4.0]undecene-5
DCA	dichloroacetamide
DCC	dicyclohexylcarbodiimide
DCM	dichloromethane
DMAP	4-dimethylaminopyridine
DME	1,2-dimethoxyethane
DMTC1	dimethoxytrityl chloride
DMSO	dimethylsulfoxide
DMTr	4, 4’-dimethoxytrityl
DMF	dimethyl formamide
EtOAc	ethyl acetate
ESI	electrospray ionization
HMDS	hexamethyldisilazane

HPLC	High pressure liquid chromatography
LDA	lithium diisopropylamide
LRMS	low resolution mass spectrum
MCPBA	meta-chloroperbenzoic acid
MeCN	acetonitrile
MeOH	methanol
MMTC	mono methoxytrityl chloride
m/z or m/e	mass to charge ratio
MH⁺	mass plus 1
MH'	mass minus 1
MsOH	methanesulfonic acid
MS or ms	mass spectrum
NBS	N-bromosuccinimide
rt or r.t.	room temperature
TBAF	tetrabutylammonium fluoride
TMSC1	chlorotrimethylsilane
TMSBr	bromotrimethylsilane
TMSI	iodotrimethylsilane
TEA	triethylamine
TBA	tributylamine
TBAP	tributylammonium pyrophosphate
TBSC1	t-butyldimethylsilyl chloride
TEAB	triethylammonium bicarbonate
TFA	trifluoroacetic acid
TLC or tic	thin layer chromatography
Tr	triphenylmethyl
Tol	4-methylbenzoyl
δ	parts per million down field from tetramethylsilane

Preparation of Compounds

Compound la-If

¹a 1b

To a solution of la (22.0 g, 54.9 mmol, prepared according to the procedures described in J.O.C., 2004,6257) in methanol (300 mL) was dropwise added acetyl chloride (22 mL) at 0 °C using a dropping funnel over a period of 30 min. and then stirred at room temperature for 16 h. The mixture was concentrated, re-dissolved in ethyl acetate (400 mL), washed with ice-cold 2 N NaOH, and concentrated to dryness, affording the crude methyl ether lb as an oil. MS = 437.2 (M + Na⁺).

1b

NaOCH₃

CH₃OH

HO OH

1c

To a solution of lb (obtained from the previous step) in methanol (300 mL) was added 0.5 M sodium methoxide solution in methanol (20 mL, 10 mmol), and stirred for 16 h at room temperature. The reaction was quenched with 4.0 N HC1 solution in dioxane (2.5 mL, 10 mmol). The mixture was then concentrated, affording the crude lc. MS = 201.0 (M + Na⁺). vn/'

^H0\AX \ / BnCI, ^κθ^Η HÔ ÔH Tritron X-405 toluene	°A!x°' CX³ m3
1c	1d

A mixture of le (obtained from the previous step), Tritron X-405 (70% in water, 6.0 g), 50% KOH (in water, 85 g) in toluene (500 mL) was heated to reflux with a Dean-Stark trap attached. After lh collecting ~25 mL of water, benzyl chloride (33 g, 260 mmol) was added and continued to reflux with stirring for 16 h. The mixture was then cooled and partitioned between ethyl acetate (400 mL) and water (300mL). The organic layer was washed with water (300 mL), and concentrated. The residue was purified by silica gel column chromatography (~20% EtOAc / hexanes), affording the methyl ether Id as an oil (22.0 g, 89% in three steps). ‘H NMR (300 MHz, CDC1₃); δ 7.3 (m, 15H), 4.5 - 4.9 (m, 7H), 4.37 (m, IH), 3.87 (d, IH), 3.56 (m, 2H), 3.52 (s, 3H), 1.40 (s, 3H).

To a solution of Id (22.0 g, 49.0 mmol) in acetic acid (110 mL) was added ~ 3

M sulfuric acid (prepared by mixing 4.8 g of concentrated sulfuric acid with 24 mL of water) and stirred at 70 °C for 8 h. The mixture was concentrated to a volume of ~20 mL, and partitioned between ethyl acetate and ice-cold 2N NaOH. The ethyl acetate layer was concentrated, and purified by silica gel column chromatography (-35% EtOAc / hexanes), affording le as an oil (17.0 g, 80%). MS = 457.2 (M + Na⁺). xa/”

1e

1f

To a solution of le (45 g, 104 mmol) in DMSO (135 mL) was dropwise added acetic anhydride (90 mL, 815 mmol) at room temperature under argon. The mixture was stirred for 16 h at room temperature, and then poured into ice-water (1 L) while stirring. After ice was completely melted (-30 min), ethyl acetate (-500 mL) was added, The organic layer was separated. This extraction process was repeated three times (3x500 mL). The organic extracts were combined and concentrated. The residue was purified by silica gel column chromatography (-20% EtOAc / hexanes), affording If as an oil (39 g, 88%). 'H NMR (300 MHz, DMSO-d₆): δ 7.3 (m, 15H), 4.4 - 4.8 (m, 7H), 4.08 (d, J= 7.5 Hz, IH), 3.75 (dd, J= 2,4, 11.4 Hz, IH), 3.64 (dd, J = 5.4, 11.4 Hz, IH), 1.51 (s, 3H).

Compound 2

Bn Bn

THF

2a

1f

To a dry, argon purged round bottom flask (100 mL) were added 7-bromopyrrolo[2,l-f|[l,2,4]triazm-4-ylamine(234 mg, 1.10 mmol) (prepared according to W02007056170) and anhydrous THF (1.5 mL). TMSC1 (276 pL, 2.2 mmol) was then added and the reaction mixture stirred for 2 h. The flask was placed into a dry ice/acetone bath (- -78 °C) and BuLi (2.5 mL, 4.0 mmol, 1.6M in hexanes) was added dropwise. After lh, a solution of If (432.5 mg, 1.0 mmol) in THF was cooled to 0 °C and then added to the reaction flask dropwise. After 1 h of stirring at -78 °C, the flask was warmed to 0 °C and sat. NH4CI (5 mL) was added to quench the reaction. The organics were extracted using EtOAc (3x10 mL) and the combined organic layers were dried using MgSO₄ The solvent was removed under reduced pressure and the crude material was purified using flash chromatography (hexanes / EtOAc). 560 mg (90 %) of 2a was isolated as a mixture of two anomers. LC/MS = 567.2 (M + H’). ^lH NMR (300 MHz, CDC1₃): δ 7.85 (m, IH), 7.27 (m, 15H), 7.01 (m, IH), 6.51 (m, IH), 4.66 (m, 8H), 4.40 (m, 2H), 3.79 (m, 3H), 1.62 (s, 2’-CH3 from the one anomer), 1.18 (s, 2’-CH₃ from the other anomer).

Alternative procedures for 2a

To a dry, argon purged round bottom flask were added 7-bromo-pyrrolo[2,lf][l,2,4]triazin-4-ylamine (9.6 g, 45 mmol) and anhydrous THF (60 mL). TMSC1 (12.4 mL, 99 mmol) was then added and the reaction mixture stirred for 2 h. The flask was placed into a dry ice/acetone bath (~ -78 °C) and BuLi (98 mL, 158 mmol, 1.6M in hexanes) was added dropwise. After 1 h, this reaction mixture was added to a solution of If (13.0 g, 30 mmol) in THF at -78 °C via cannula. After 2 h of stirring at -78 °C, the flask was warmed to 0 °C. Saturated NH4CI (150 mL) was added to quench the reaction. The organics were extracted using EtOAc (3 x 100 mL) and the combined organic layers were dried using MgSO4 The solvent was removed under reduced pressure and the crude material was purified using flash silica gel chromatography (hexanes / EtOAc). 7.5 g (44 %) of the desired material 2a was isolated. LC/MS - 567.2 (M 1 li').

Compound 5

To a solution of compound 2a (1 g, 1.77 mmol) in CH₂C1₂ (20 mL) at 0 °C was added TMSCN (1.4 mL, 10.5 mmol) and BF₃-Et₂O (1 mL, 8.1 mmol). The reaction mixture was stirred at 0 °C for 0.5 h, then at room temperature for additional 0.5 h. The reaction was quenched with NalICO-, at 0 °C, and diluted with CH₃CO₂Et. The organic phase was separated, washed with brine, dried over Na₂SC>4, filtered and concentrated. The residue was purified by chromatography on silica gel, eluted with CH₃CO₂Et-hexanes (1:1 to 2:1), to give the desired compound 5a (620 mg, 61 %) as an isomeric mixture. MS = 576.1 (M + H⁺).

5a 5

To a solution of compound 5a (150 mg, 0.26 mmol) in CH2CI2 (4 mL) at -78 °C was added BC1₃ (2 mL, IM in CH₂C12). The reaction mixture was stirred at -78 °C for 1 h. The reaction was quenched at -78 °C by dropwise addition of TEA (2 mL) and MeOH (5 mL). The mixture was allowed to warm up to room temperature, evaporated, and co-evaporated with MeOH several times. The residue was treated with NaHCO₃ (I g in 10 mL H₂O), concentrated and purified by HPLC to give the desired product Compound 5 (48 mg, 60%). ^lH NMR (300 MHz, D2O): δ 7.74 (s IH), 6.76 (d, J = 5 Hz, 1H), 6.73 (d, J = 5 Hz, IH), 4.1 (m, IH), 3.9 (m, IH), 3.8 (m, 2H), 0.84 (s, 3H). MS = 305.9 (M + H⁺). The other alpha-anomer was also obtained (9 mg, 11%): ^lH NMR (300 MHz, D2O): δ 7.70 (s IH), 6.8 (d, J = 5 Hz, IH), 6.7 (d, J = 5 Hz, IH), 4.25 (d, J “ 9 Hz, IH), 4.07 (m, IH), 3.85 (m, IH), 3.7 (m, IH), 1.6 (s, 3H). MS = 306.1 (M + H⁺).

O

II

CI-P-CI

A

TEA I CH₂CI₂

Compound A (commercially available, 4.99 g, 23.8 mmol) was dissolved in dichloromethane (100 mL) and alanine isopropyl ester hydrochloride (3.98 g, 23.8 mmol) was added. The resulting clear solution was cooled -78 °C for 30 min. Triethylamine (6.63 mL, 47.5 mmol) was added dropwise over 15 min. The mixture was then allowed to warm to room temperature. After 16 h, the solvent was removed by argon stream. The residue was re-dissolved in MTBE (25 mL) and the insoluble was removed by filtration under argon. The filtrate was then condensed by argon stream and the crude product B was used for the next reaction without further purification. 'H NMR (300 MHz, CDC1₃): 7.1-7.4 (m, 5H), 5.1 (m, IH), 4.35 (m, IH), 4.15 (m, IH), 1.5 (d, 3H), 1.2(m,6H). ^3IP NMR (121.4 MHz, CDC1₃): δ 7.8 and 8.4 (2s).

HCI

Ο ci—p—ci

p-Nîtrophenol

TEA / CH₂CI_Z

Alanine isopropyl ester hydrochloride (7.95 g, 47.4 mmol) was suspended in dichioromethane (100 mL). Compound A (IO g, 47.4 mmol) was added. Triethylamine (13.2 mL, 95 mmol) was then dropwise added over a period of 15 min.

(internal reaction temperature; - IO °C ~ -3 °C). When the reaction was almost complete (by phosphorous NMR), p-nitrophenol (6.29 g, 45.0 mmol) was added as a solid in one portion. To the resulting slurry was added tri ethylamine (6.28 mL, 45 mmol) over a period of 15 min. The mixture was then warmed up to room temperature. When the reaction was complete, MTBE (100 mL) was added. The

IO white precipitate was removed by filtration. The filter cake was washed with MTBE (3x50 mL). The filtrate and washings were combined and concentrated. The residue was purified by silica gel column chromatography (0 to 50% ethyl acetate / hexanes), affording compound C as a 1:1 ratio of diasteromeric mixture (14.1 g, 77%). '11 NMR (300 MHz, CDC1₃): δ 8.22 (2d, 2H), 7.2-7.4 (m, 7H), 5.0 (m, IH), 4.09 (m, lH),3.96(m, IH), 1.39 (2d, 3H), 1.22 (m,6H). MS = 409.0 (M + H⁺), 407.2 (MH⁺).

Separation of two diatereomers of compound C

diastereomeric mixture at phosphorous

The two diastereomers were separated by chiral column chromatography under the following conditions;

Column; Chiralpak IC, 2 x 25 cm

Solvent system: 70% heptane and 30% isopropanol (IPA)

Flow rate: 6 mL/min.

Loading volume per run: 1.0 mL

Concentration of loading sample: 150 mg/ mL in 70% hepane and 30% IPA (S)-compound C: retention time 43 min. ³¹P NMR (161.9 MHz, CDC13): δ -2.99 (s). (R)-compound C: retention time 62 min. ³¹P NMR (161.9 MHz, CDC13): δ -3.02 (s).

Alternatively, the two diasteromers were separated by crystallization under the following procedures;

Compound C was dissolved in diethyl ether (--10 mL/ gram). While stirring, hexanes was then added until the solution became turbid. Seed crystals (~10 mg / gram of compound C) were added to promote crystallization. The resulting suspension was gently stirred for 16 h, cooled to ~ 0 °C, stirred for an additional 2 h, and filtered to collect the crystalline material (recovery yield of the crystalline material 35%-35% The crystalline material contains -95% of (S)-compound C and w/* ~5% of (R)-compound C. Re-crystallization afforded 99% diastereomerically pure (S)-isomer.

Compound (S)-6

(S)-C t-BuMgCI

THF

To a dry, argon purged round-bottom flask were added compound 5 (1.0 g, 3.28 mmol) and anhydrous THF (15 mL). The slurry was stirred for 10 min. and the flask was place in a water bath at room temperature, i-Butyl magnesium chloride in

THF (1.0 M, 4.91 mL) was dropwise added, and the mixture was stirred for an additional 10 min. A solution of (S)-C (2.68 g, 6.55 mmol) in THF (10 mL) was then added. The flask was place in a heating oil bath pre-set at 50 oC and the mixture was stirred until compound 1 was almost consumed. After ~2.5 h, the reaction mixture was cooled to room temperature, and methanol (5 mL) was added. Solvents were removed under reduced pressure and the residue was purified by silica gel column chromatography (70% ethyl acetare / hexanes to remove less polar impurities, 10% methanol ! dichloromethane to elute the product), affording (S)-6 as an off-white solid (1.45 g, 77%). ’H NMR (400 MHz, DMSO-d₆): δ 7.89 (s, 1H), 7.84 (brs, 2H), 7.36 (t, 2H), 7.23 (d, 2H), 7.17 (t, 1H), 6.87 (d, J= 4.4 Hz, 1H), 6.74 (d, J= 4.4 Hz, 1H),

6.02 (dd, 1H), 5.96 (s, 1H), 5.41 (d, 1H), 4.82 (m, 1H), 4.38 (dd, 1H), 4.22 (q, 1H),

4.16 (m, 1H), 3.81 (m, 1H), 3.67 (dd, 1H), 1.22 (d, 3H), 1.11 (dd, 6H), 0.89 (s, 3H). ³¹P NMR (161.9 MHz, DMSO-d₆): δ 3.99 (s). MS = 575.0 (M + H⁺), 572.7 (M - H⁺).

Compound (S)-8

(S)-C t-BuMgCI

NMP/THF

To a dry, argon purged round-bottom flask were added compound 7 (prepared according to J. Med. Chem., 2005, 48, 5504-5508, 100 mg, 0.38 mmol), anhydrous THF (3 mL) and anhydrous NMP (1 mL). The slurry was stirred for 10 min. and the flask was place in a water bath at room temperature. Z-Butylmagnesium chloride in THF (1.0 M, 0.76 mL) was dropwise added, and the mixture was stirred for an additional 10 min. A solution of (S)-C (313 mg, 0.76 mmol) in THF (2 mL) was then added. The flask was place in a heating oil bath pre-set at 55 °C and the mixture was stirred until compound 7 was almost consumed. After -2.5 h. the reaction mixture was cooled to room temperature, and methanol (1 mL) was added. Solvents were removed under reduced pressure and the residue was purified by RP-HPLC followed by silica gel column chromatography, affording (S)-8 (130 mg, 65%). *H NMR (400 MHz, CDCh): δ 8.51 (brs, IH), 7.46 (d, IH), 7.2-7.4 (m, 5H), 6.28 (d, IH), 5.70 (dd, IH), 5.01 (m, IH), 4.49 (m, 2H), 3.8-4.I (m,4H), 1.41 (d, 3H), 1.35 (d, 3H), 1.24 (d, 6H). ³¹P NMR (162.1 MHz, CDC13): δ 3.70 (s). MS - 530.0 (M + H⁺), 528.0 (M H⁺). Chiral HPLC retention time (Chiralpak AS-H, 250 x 4.6 mm 5 micron, 100% CH3CN, 1 mL/min flow rate); 6.5 min vs. 5.2 min for the R-isomer).

Using the general procedures described for the preparation of Compound (5)C or Compound (/?)-(’, Compounds 10-24 may be prepared.

Compound 11

Compound 12

Compound 13

Compound 16

Compound 17

Compound 18

Compound 19

Compound 22

Compound 23

Compound 24

Using the general procedures described for the preparation of Compound (S)6, Compounds 25-38 may be prepared using either Compound (S)-C or Compound sa/*'' (R)-C.

ΙΟ

Compound 29

Compound 32

Compound 35

Compound 36

Compound 37

Claims

What is claimed is

I, A method for preparing a compound of Formula la or lb:

Formula la

R²² R²

Formula lb or a pharmaceutically acceptable salt or acid thereof;

wherein:

5 each R¹, R², R⁷, R²², R²³ or R²⁴ is independently H, OR¹¹, NR¹¹R¹²,

CtOiNR^R¹², -OC(O)NRⁿR¹², C(O)ORⁿ, OC(O)OR^1!, S(O)nR^a.S(O)2NR¹¹R¹², N₃, CN, halogen, (Ci-Cg)alkyl, (C₃-Cg)carbocyclyl, (C4~C₈)carbocyclylalkyl, (C₂-Cg)alkenyl, (C₂-C_g)alkynyl or aryl(C!-Cg)alkyl;

or any two R , R , R , R , R or R on adjacent carbon atoms when taken

10 together are -O(CO)O- or -O(CR' 'R¹²)O- or when taken together with the ring carbon atoms to which they are attached form a double bond; vZ each Base is independently a naturally occurring or modified purine or pyrimidine base linked to the furanose ring through a carbon or nitrogen atom;

provided that Base is not uracil;

each n is independently 0,1, or 2;

each R^a, R⁴ or R⁶ is independently (C]-Cg)alkyl, (C₂-Cg)alkenyl, (C₂Cg)alkynyl, (C₃-Cg)carbocyclyl, (C.r ('s)carbocyclylalkyl, aryl(Cj-Cg)alky 1, heterocyclyl(Ci-Cg)alkyl, ((7<C?o)aryL (C₂-C₂o)heterocyclyl or heteroaryl;

each R^c or R^d is independently H, (Ci-Cg)alkyl, (C₂-Cs)alkenyl, (C₂Cg)alkynyl, (C₃-Cg)carbocyclyl, (C₄-Cg)carbocyclylalkyl, aryl(C]-Cg)alkyl, heterocyclyl(C|-C8)alkyl, (C6-C₂₀)aryl, (C₂-C₂o)heterocyclyl or heteroaryl provided that R^c and R^d are not the same;

each R⁵ is independently H, (Ci-Cg)alkyl, (C₂-Cg)alkenyl, (C₂-Cg)alkynyl, (C₃C₈)carbocyclyl, {C₄--Cg)carbocyclylalkyl, aryl(Ci-Cg)alkyl, heterocyclyl(Ci-Cs)alkyl, (C6-C₂o)aryl, (C₂-C₂o)heterocyclyl or heteroaryl;

each R¹¹ or R¹² is independently H, (Ci-Cg)alkyl, (C₂-Cg)alkenyl, (C₂Cg)alkynyl, (C₃-Cg)carbocyclyl, (C₄-Cg)carbocyclylalkyl, aryl(C|-Cg)alkyl, heterocyclyl(C]-Cs)alkyl, (C6-C₂o)aryl, (C₂-C₂o)heterocyclyl, heteroaryl, -C(=O)(CiCg)alkyl, -S(O)_n(Ci-Cg)aIkyI or R¹¹ and R¹² taken together with a nitrogen to which they are both attached form a 3 to 7 membered heterocyclic ring wherein any one carbon atom of said heterocyclic ring can optionally be replaced with -0-, -S(O)_n- or ~NR^a-; and wherein each (Cj-Cg)alkyl, (C₂-Cg)alkenyl, (C₂-C_g)alkynyl, (C₃C₈)carbocyclyl, (C₄-C8)carbocyclylalkyl, arylfCi-Cgjalkyl, heterocyclyl(C]-Cg)alkyl, (C6~C₂o)aryl, (C₂-C₂o)heterocyclyl or heteroaryl of each R^c, R^d, R¹, R², R²², R²³, R²⁴, R⁴, R⁵, R⁶, R⁷, R’¹ or R¹² is, independently, optionally substituted with one or more halo, hydroxy, CN, N3, N(R^a)2, NH(R^a), NH2, NO2, C(O)N(R^a)2, C(0)NH(R^a), C(O)NH2, OC(O)N(R^a)2, OC(O)NH(R^a), OC(O)NH2, C(O)OR^a, OC(O)OR^a, C(O)R^a, OC(O)R^a, S(O)nR^a S(O)2N(R^a)2, S(O)₂NH(R^a), S(O)₂NH₂, OR^a or R^a:

said method comprising :

(a) providing a compound of Formula II

Formula Π and (b) treating the compound of Formula II with a compound of Formula Illa and

abase 0 R⁵ II 1 'Jyv _Rd R^c \ R⁴O Ar 5 Formula Illa

thereby forming a compound of Formula la or (c) treating the compound of Formula II with a compound of Formula Illb and a base

Formula Illb thereby forming a compound of Formula lb;

wherein: each Ar is a (C6~C₂o) aryl or heteroaryl wherein said (C₆-C2o) aryl or heteroaryl is substituted with one or more halogen, NO-. or (Ci-Cs)haloalkyl and optionally substituted with with one or more CN, N₃, N(R^a)2, C(O)N(R^a)₂,

15 OC(O)N(R^a)2, C(O)OR^a, OC(O)OR^a, C(O)R^a, OC(0)R^a, S(O)nR^a, S(O)2N(R^a)2, OR^a or R^a with the proviso that Ar is different from R⁴.
2. The method of claim 1 wherein Formula la is Formula IVa, Formula lb is

Formula IVb and Formula II is Formula V:

R²² R²
3. The method of claim 1 or 2 wherein Base is selected from the group consisting

5 of: vxÆ wherein:

each X¹ is independently N or CR¹⁰;

each X² is independently NR¹¹,0, or S(O)_n;

each R⁸ is independently halogen, NRⁿR¹², N(R^ll)OR¹¹, NRⁿNRⁿR¹², Nj, NO, NO2, CHO, CN, -CH(=NR^1]), -CH=NNHRⁿ, -('HNfOR¹'), -CH(OR^h)2, -C(=O)NR^1!R¹², -C(=S)NRⁿR¹², -C(=O)OR¹¹, (Ci-Cg)alkyl, (C₂-C₈)alkenyl, (C₂C₈)alkynyl, (C₄-C₈)carbocyclylalkyl, (Cû-C₂o)aryl, (C₂-C₂₀)heterocyclyl, heteroaryl, -C(=O)(C[-C₈)aIkyl, -S(O)_n(Ci-C₈)alkyl, aryl(C_rC₈)alkyl, OR¹¹ or SR¹¹;

each n is independently 0, 1, or 2;

each R⁹ or R¹⁰ is independently H, halogen, NRⁿR¹², NiR’^OR¹¹, NRⁿNR^llR¹², N3, NO, NO2, CHO, CN, -CH(=NRⁿ), -CH^NHNR¹¹, -CH=N(OR¹¹), -CH(ORⁿ)2, -C(=O)NR^hR¹², -C(=S)NR^liR¹², -C(=O)OR^U, R¹¹, OR¹¹ or SR¹¹;

each R¹¹ or R¹² is independently H, (Ci-C8)alkyl, (C2-C8)alkenyl, (C2C8)alkynyl, (C3-C8)carbocyclyl, (C4-C8)carbocyclylalkyl, aryl(Ci-C8)alkyl, heterocyclyl(C[-C8)alkyl, (C6C?l))aryL (C2-C2o)heterocyclyl, heteroaryl, -C(=O)(Cr C8)alkyl, -S(O)n(Ci-C8)alkyl or R¹¹ and R¹² taken together with a nitrogen to which they are both attached form a 3 to 7 membered heterocyclic ring wherein any one carbon atom of said heterocyclic ring can optionally be replaced with -O-, -S(O)n- or -NR^a-;

wherein each (C]-C₈)alkyl, (C₂-C₈)alkenyl, (C₂-C₈)alkynyl, (C₃C₈)carbocyclyl, (C₄ C₈)carbocyclylalkyl, aryl(Ct-C₈)alkyl, heterocyclyl(Ci-C₈)alkyi, (C6-C₂o)aryl, (C₂-C₂o)heterocyclyl or heteroaryl of each R^c, R^d, R¹, R², R²², R²³, R²⁴,

R⁴, R^s, R⁶, R⁷, R⁸, R⁹, R¹⁰, R¹¹ or R¹² is, independently, optionally substituted with one or more halo, hydroxy, CN, N3, N(R^a)2, NH(R^a), NH2, NO2, C(O)N(R^a)2, C(O)NH(R^a), C(O)NH2, OC(O)N(R^a)2, OC(O)NH(R^a), OC(O)NH2, C(O)OR^a, OC(O)OR^a, C(O)R^a, OC(O)R^a, S(O)nR^a, S(O)2N(R^ah, S(O)2NH(R^a), S(O)₂NH₂, OR^a5 or R^a; and provided that Base is not uracil.
4. The method of any one of claims 1-3 wherein Base is selected from the group consisting of:

10 5. The method of any one of claims 1 -3 wherein Formula la is Formula Via,

Formula lb is Formula Vlb and Formula II is Formula VII:

Formula Via

Formula VIb

R⁸

Formula VII

6. The method of any one of claims I -3 wherein Formula la is Formula Xia,

Formula lb is Formula Xlb and Formula II is Formula XII:

R²² R²

Formula Xlb

R⁰

R²² R²

Formula XII wherein:

each R¹ is independently H, halogen, optionally substituted (Ci-Cs)alkyl, optionally substituted (C2-Cg)alkenyl or optionally substituted (C? -Cg)alkynyl;

Π * !

each R is independently halogen or OR ;

each R⁵ is H; and each R²² is OR¹¹.

7. The method of any one of claims 1 -3 wherein Formula la is Formula XHIa,

Formula lb is Formula XHIb and Formula II is Formula XIV:

Formula XHIa _R22

Formula XHIb

Formula XIV wherein:

each R¹ is independently H, halogen, optionally substituted (Cj--Cs)alkyl, optionally substituted (C₂=C_g)alkenyl or optionally substituted (C2-C₈)alkynyl;
5 each R² is independently halogen or OR*¹ ;

each R⁵ is H; and each R²² is OR¹¹.
8. The method of any one of claims 1 -7 wherein R¹ is H, halogen, optionally substituted (C|Cs)alkyl, optionally substituted (C2-C_g)alkenyl or optionally

1 o substituted (C2-C_g)alkynyl.
9. The method of any one of claims 1 -8 wherein R¹ is H, CH₃ or F.
10. The method of any one of claims 1-9 wherein each R⁵, R²³ and R²⁴ is H.
11. The method of any one of claims 1-10 wherein one of R^c or R^d is H and the other of R^c or R^d is optionally substituted (Ci-C_g)alkyl.

15
12. The method of any one of claims 1-11 wherein R⁶ is optionally substituted (Ci-C₈)alkyl.
13. The method of any one of claims 1 - 12 wherein R⁸ is NR¹ *R¹² or OR¹¹.
14. The method of any one of claims 1-13 wherein R⁹ is H or NR¹ *R¹².
15. The method of any one of claims 1-14 wherein when R⁸ or R⁹ is OR¹¹ or

NR“R¹² then eachR¹¹ andR¹² of said OR¹¹ or NR^UR¹² is H.

5
16. The method of any one of claims 1-15 wherein R⁸ is NH₂ and R⁹ is H.
17. The method of any one of claims 1-16 wherein R⁴ is optionally substituted (C₆-C₂₀)aryl.
18. The method of any one of claims 1-17 further comprising a method of preparing a compound of Formula Illa or Formula Illb wherein:

each R^a, R⁴ or R⁶ is independently (Ci-C₈)alkyl, (C2-Cg)alkenyl, (C₂C₈)alkynyl, (Ci-Cs)carbocyclyl, (C4- -Cg)carbocyclylalkyl, aryl(Ci-C₈)alkyl, heterocycIyl(Ci-C₈)alkyl, (C&--C20)aryl, (C2-C2o)heterocyclyl or heteroaryl;

15 each R^c or R^d is independently H, (Ci-Cg)alkyl, ((T-CjJalkenyl, (C₂C₈)alkynyl, (C₃-C₈)carbocyclyl, (C₄C«)carbocyclylalkyl, aryl(Ci-C_s)alkyl, heterocyclyl(Ci-Cg)alkyl, (C/Qt/aryl. (C> -C₂o)heterocyclyl or heteroaryl provided that R^c and R^d are not the same;

each R⁵ is independently H, (Cj-C_s)alkyl, (C₂-C₈)alkenyl, (C₂-C_g)alkynyl, (C₃Cg)carbocyclyl, (C4-Cg)carbocyclylalkyl, aryl(Ci-C_g)alkyl, heterocyclyl(Ci-ChalkyI, ⁵ ((/, C^cjaryl, (C₂-C₂₀)heterocyclyl or heteroaryl;

wherein each (C|-C«)alkyl, (C₂-Cg)alkenyl, (C₂-Cs)alkynyl, (C₃Cgjcarbocyclyl, (C₄-C8)carbocyclylalkyl, arylfCi-CgJalkyl, heterocyclyl(C|-C8)alkyl, (C₆-C₂o)aryl, (C₂-C₂o)heterocyclyl or heteroaryl of each R^c, R^d, R⁴, R⁵ or R⁶ is, independently, optionally substituted with one or more halo, hydroxy, CN, N3, N(R^a)2, 10 NH(R^a), NH2, C(O)N(R^a)2, C(O)NH(R^a), C(O)NH2, OC(O)N(R^a)2, OC(O)NH(R^a),

OC(O)NH₂, C(O)OR^a, OC(O)OR^a, S(O)_nR^a S(O)2N(R^a)2, S(O)₂NH(R^a), S(O)₂NH₂, OR^a or R^a; and each Ar is a (C6-C₂o) aryl or heteroaryl wherein said (C6-C₂o) aryl or heteroaryl is substituted with one or more halogen, NO₂, or (Ci-Cg)haloalkyl and 15 optionally substituted with with one or more CN, N₃, N(R^a)2. C(O)N(R^a)2,

OC(O)N(R^a)2, C(O)OR^a, OC(O)OR^a, C(O)R^a, OC(O)R^a, S(O)nR^a, S(O)2N(R^a)2, OR^aor R^a with the proviso that Ar is different from R⁴;

said method comprising:

(d) providing a diastereomeric compound of Formula VIII and (e) dissolving the compound of Formula VIII in a suitable solvent and inducing crystallization by addition of a Cs-Cg hydrocarbon or Cs-Cs cyclic hydrocarbon;

thereby forming a pure diasteromer of Formula Illa or Formula Illb.

100
19. The method of claim 18 wherein the compound of Formula VIII is dissolved in an ether solvent and the crystallization is induced by addition of a Cg-Cg hydrocarbon.
20. The method of claim 18 or 19 wherein the compound of Formula VIII is ⁵ dissolved in diethyl ether or methyl-t-butyl ether and crystallization is induced by the addition of hexane.
21. The method of any one of claims 18-20 wherein the compound of Formula VIII is dissolved in diethyl ether and crystallization is induced by the addition of hexane.

10
22. The method of any one of claims 18-21 further comprising a method of preparing a compound of Formula VIII wherein:

each R^a, R⁴ or R⁶ is independently (Ci-Cg)alkyl, (CVC_s)a]kenyl, (C₂C₈)alkynyl, (CrC₈)carbocyclyl, (C₄-C₈)carbocyclylalkyl, aryl(Ci-Cg)alkyl, heterocyclyl(Ci-C₈)alkyl, (C6-C₂₀)aryl, (C₂-C₂o)heterocyclyl or heteroaryl;

each R^c or R^d is independently H, (Ci-C₈)alkyl, (C₂-C₈)alkenyl, (C₂C₈)alkynyl, (C3-C₈)carbocyclyl, (C₄-C₈)carbocyclylalkyl, aryl(Ci-C₈)alkyl, heterocyclyl(C|-C₈)alkyI, (C6-C₂₀)aryl, (C₂-C₂₀)heterocyclyl or heteroaryl provided that R^c and R^d are not the same; \a/

101 each R⁵ is independently IL (Ci-Cgjalkyl, (C₂-C₈)alkenyl, (C₂-C₈)alkynyl, (C₃( 's)carbocvciyl, (C₄-C₈)carbocyclylalkyl, aryl(C|-C₈)alkyl, heterocyclyl(Ci-C₈)alkyl, (Cs-C₂₀)aryl, (C₂-C₂₀)heterocyclyl or heteroaryl;

wherein each (C_rC₈)alkyl, (C₂-C₈)alkenyl, (C₂-C₈)alkynyl, (C₃C₈)carbocyclyl, (C₄-C₈)carbocyclylalkyl, aryI(C|-C₈)alkyl, heterocyclyl(Ci-C₈)alkyl, (C6-C₂o)aryl, (C₂-C₂o)heterocyclyl or heteroaryl of each R^c, R^d, R⁴, R³ or R⁶ is, independently, optionally substituted with one or more halo, hydroxy, CN, N3, N(R^a)2> NH(R^a), NH2, C(O)N(R^a)2, C(O)NH(R^a), C(O)NH2, OC(O)N(R^a)2, OC(0)NH(R^a), OC(O)NH2, C(O)OR^a, OC(O)OR^a, S(O)nR^a, S(O)2N(R^a)2, S(O)₂NH(R^a), S(O)₂NH₂, OR^a or R^a; and each Ar is a (C₍, -CY) aryl or heteroaryl wherein said (Cé-C₂₀) aryl or heteroaryl is substituted with one or more halogen, NO₂, or (Ci-C₈)haloalkyl and optionally substituted with with one or more CN, N₃, N(R^a)2> C(O)N(R^a)2, OC(O)N(R^a)2, C(O)OR^a, OC(O)OR^a, C(O)R^a, OC(O)R^a, S(O)nR^a, S(O)2N(R^a)2, OR^aor R^a with the proviso that Ar is different from R⁴;

said method comprising:

(f) providing a chirally pure amino acid ester of Formula IX or a salt thereof (g) treating the compound of Formula IX with a compound of Formula X in the presence of a base

Formula X wherein each X³ is halogen; and (h) treating the resulting mixture with ArOH; thereby forming a compound of Formula VIII.

102
23. A method of preparing a compound of Formula Illa or Formula IHb or a salt or ester thereof, wherein:

5 each R^a, R⁴ or R⁶ is independently (Ci-C₈)alkyl, (C₂-C₈)alkenyl, (C₂Cs)alkynvl, (C₃-C₈)carbocyclyl, (C4-C₈)carbocyclylalkyl, aryl(C|-Q)aikyl, heterocyclyl(Ci-C₈)alkyl, (C₆-C₂o)aryl, (C₂-C₂o)heterocyclyl or hetero aryl;

each R^c or R^d is independently H, (Ci-Cg)alkyl, (C₂-C₈)alkenyl, (C₂C₈)alkynyl, (C₃-C₈)carbocyclyl, (C4-C₈)carbocyclylalkyl, aryl(Ci~C₈)alkyl,

10 heterocyclyl(C]-C₈)alkyl, (C₍₎--C₂n)aryi, (C₂-C₂o)heterocyclyl or heteroaryl provided that R^c and R^d are not the same;

each R⁵ is independently H, (C|-Cg)alkyl, (C₂-C₈)alkenyl, (C₂-C₈)alkynyl, (C _rC₈)carbocyclyl, (C4-C₈)carbocyclylalkyl, aryl(Ci-C₈)alkyl, heterocyclyl(Cj-C₈)alkyl, (C₆-C₂₀)aryI, (C₂-C₂₀)heterocyclyl or heteroaryl;

15 wherein each (Ci-C₈)alkyl, (C₂-C₈)alkenyl, (C₂-C₈)alkynyl, (C₃C₈)carbocyclyl, (C% C_s)carbocyclylalkyl, aryl(Ci-C₈)alkyl, heterocyclyl.(Cj-Cgjalkyl, (OC₂o)aryl, (C₂~C₂o)heterocyclyl or heteroaryl of each R^c, R^d, R⁴, R⁵ or R⁶ is, independently, optionally substituted with one or more halo, hydroxy, CN, N3, N(R^a)2t NH(R^a), NH2, C(O)N(R^a)2, C(O)NH(R^a), C(O)NH2, OC(O)N(R^a)2, OC(O)NH(R^a),

103 «

*16292

OC(O)NH₂, C(O)0R, OC(O)OR·, S(O)„R·. S(O)₂N(R')₂, S(O)₂NH(R), S(O)₂NH_2iOR³ or R^a; and each Ar is a (QGi) aryl or heteroaryl wherein said (C6-C20) aryl or heteroaryl is substituted with one or more halogen, NO₂, or (C;-Cg) halo alkyl and optionally substituted with with one or more CN, N₃, N(R^a)2 C(O)N(R^a)2, OC(O)N(R^a)2, C(O)OR^a, OC(O)OR^a, C(O)R^a, OC(O)R^a, S(O)nR^a S(O)2N(R^a)2, OR^aor R^a with the proviso that Ar is different from R⁴;

said method comprising:

(d) providing a diastereomeric compound of Formula VIII and (e) dissolving the compound of Formula VII] in a suitable solvent and inducing crystallization by addition of a C₅-Cg hydrocarbon or Cs-Cg cyclic hydrocarbon;

thereby forming a pure diasteromer of Formula Illa or Formula Illb.
24. The method of claim 23 wherein the compound of Formula VIII is dissolved in an ether solvent and the crystallization is induced by addition of a Cs-Cg hydrocarbon.
25. The method of claim 23 or 24 wherein the compound of Formula VIII is dissolved in diethyl ether or methyl-t-butyl ether and crystallization is induced by the addition of hexane.
26. The method of any one of claims 23-25 wherein the compound of Formula VIII is dissolved in diethyl ether and crystallization is induced by the addition of hexane. W’

104
27. The method of any one of claims 23-26 further comprising a method of preparing a compound of Formula VIII

Formula VIII or a salt or ester thereof, wherein each R^a, R⁴ or R⁶ is independently (C]-C₈)alkyl, (C₂-Cg)alkenyl, (C₂Cg)alkynyl, (C₃-Cg)carbocyclyl, (C4-Cg)carbocyclylalkyl, aryl(Cj-Cg)alkyl, heterocyclyI(Ci-Cg)alkyl, (C6-C₂o)aryl, (C₂-C2o)heterocyclyl or heteroaryl;

each R^c or R^d is independently H, (Ci-C₈)alkyl, (C_?-C\)aikcnyl, (C-10

Cg)alkynyl, (C₃-Cg)carbocyclyl, (C4-Cg)carbocyclylalkyl, aryl(C]-Cg)alkyl, heterocycIyl(Ci-Cg)alkyl, (C₆-C₂o)aryl, (C₂-C₂o)heterocyclyl or heteroaryl provided that R^c and R^d are not the same;

each R⁵ is independently H, (Ci-Cg)alkyl, (C₂-Cg)alkenyl, (C₂-Cg)alkynyl, (C₃Cg)carbocyclyl, (C4-Cg)carbocyclylalkyl, aryl(Ci-Cg)alkyl, heterocyclyl(C]-Cg)alkyl, (C6-C2o)aryl, (C₂-C₂o)heterocyclyl or heteroaryl;

wherein each (Ci-Cg)alkyl, (C₂-Cg)alkenyl, (C₂-Cg)alkynyl, (C₃C₈)carbocyclyl, (C4-Cg)carbocyclylalkyl, aryl(Ci-Cg)alkyl, heterocyclyl(Cj-Cg)alkyl, (C6-C₂o)aryl, (C₂-C₂o)heterocyclyl or heteroaryl of each R^c, R^d, R⁴, R⁵ or R⁶ is, independently, optionally substituted with one or more halo, hydroxy, CN, N3, N(R^a)2, NH(R^a), NH2, C(O)N(R^a)2, C(O)NH(R^a), C(O)NH2, OC(O)N(R^a)2, OC(O)NH(R^a), OC(O)NH₂, C(O)OR^a, OC(0)OR^a, S(O)„R^a, S(O)2N(R^a)2, S(O)₂NH(R^a), S(O)₂NH₂, OR^a or R^a; and each Ar is a (C6-C₂o) aryl^or heteroaryl wherein said (C6-C2o) aryl or heteroaryl is substituted with one or more halogen, NO2, or (Ci-Cg)haloalkyl and optionally substituted with with one or more CN, N3, N(R^a)? C(O)N(R^a)₂,

105

OC(O)N(R^a)2, C(O)OR^a, OC(O)OR^a, C(O)R^a, OC(O)R^a, S(O)nR^a, S(O)2N(R^a)2, OR^aor R^a with the proviso that Ar is different from R⁴;

said method comprising:

(f) providing a chirally pure amino acid ester of Formula IX or a salt thereof (g) treating the compound of Formula IX with a compound of Formula X in the presence of a base χ3

Formula X wherein each X³ is halogen; and (h) treating the resulting mixture with ArOH; thereby forming a compound of Formula VIII.
28. The method of any one of claims 18-27 wherein the compound Formula Illa or Formula Illb is

106

Formula Illb or a salt or ester thereof;

wherein:

each R^a, R⁴ or R⁶ is independently (Ci-CQalkyk (C₂-C₈)alkenyl, (C₂C₈)alkynyl, (C₃-Cs)carbocyclyl, (C₄-C₈)carbocyclylalkyl, aryl(Ci-C₈)alkyl, heterocyclyl(Ci-C₈)alkyl, (Q-CNjjaryl, (C2-C2o)heterocyclyl or heteroaryl;

each R^c or R^d is independently H, (Ci-C₈)alkyl, (C2-C₈)alkenyl, (C₂C₈)alkynyl, (C₃-C₈)carbocyclyl, (C4-C₈)carbocyclylalkyl, aryl(Ci-C₈)alkyl, heterocyclyl(Ci-C₈)alkyl, (C6-C₂o)aryl, (C₂-C₂o)heterocyclyl or heteroaryl provided that R^c and R^d are not the same;

each R⁵ is independently H, (Ci-C₈)alkyl, (C₂-C₈)alkenyl, (C₂-C₈)alkynyl, (C₃C₈)carbocyclyl, (C4-C₈)carbocyclylalkyl, aryI(Ci-C₈)alkyl, heterocyclyl(Ci-C₈)alkyl, (C6-C₂o)aryl, (C₂-C₂o)heterocyclyl or heteroaryl;

wherein each (CrCg)alkyl, (C₂-C₈)alkenyl, (C₂-C₈)alkynyl, (C₃C₈)carbocyclyl, (C4-C₈)carbocyclylalkyl, aryl(Ci-C₈)alkyl, heterocyclyl(C[-C₈)alkyl, (C6~C₂o)aryI, (C₂-C₂o)heterocyclyl or heteroaryl of each R^c, R^d, R⁴, R⁵ or R⁶ is, independently, optionally substituted with one or more halo, hydroxy, CN, N3, N(R^a)2> NH(R^a), NH2, C(O)N(R^a)2, C(O)NH(R^a), C(O)NH2, OC(O)N(R^a)2, OC(O)NH(R^a), OC(O)NH2, C(O)OR^a, OC(O)OR^a, S(O)nR^a. S(O)₂N(R^a)2, S(O)2NH(R^a), S(O)2NH₂, OR^a or R^a; and each Ar is a (Q-C20) aryl or heteroaryl wherein said (C₆-C₂o) aryl or heteroaryl is substituted with one or more halogen, NO₂, or (C]-C₈)haloalkyl and optionally substituted with with one or more CN, N₃, N(R^a)2, C(O)N(R^a)2, OC(O)N(R^a)2, C(O)OR^a, OC(O)OR^a, C(O)R^a, OC(O)R^a, S(O)nR^a, S(O)2N(R^a)2, OR^aor R^a with the proviso that Ar is different from R⁴.
29. The method of any one of claims 18-28 wherein R⁵ is H and one of R^c or R^d is Η.

107
30. The method of any one of claims 18-29 wherein R⁶ is optionally substituted (Cj-Cg)alkyl and R⁴ is optionally substituted (Cf-CNoiaryl.
31. The method of any one of claims 18-30 wherein R⁴ is optionally substituted phenyl.

5
32. The method of any one of claims 18-31 wherein one of R^c or R^d is H and the other of R^c or R^d is CH₃.
33. The method of any one of claims 18-32 wherein Ar is optionally substituted para-nitrophenyL
34. The method of any one of claims 18-33 wherein the chirality at the carbon

10 directly attached to R^c and R^d is S.
35. The method of any one of claims 18-34 wherein the chirality at the carbon directly attached to R^c and R^d is R.
36. The method of any one of claims 18-35 wherein the compound is selected from the group consisting of ϊ 1

108 esters thereof

109