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NL7403633A - Automatic determination of triglycerides in serum - using a blank comprising serum contg. hydrolysed triglycerides - Google Patents

Automatic determination of triglycerides in serum - using a blank comprising serum contg. hydrolysed triglycerides

Info

Publication number
NL7403633A
NL7403633A NL7403633A NL7403633A NL7403633A NL 7403633 A NL7403633 A NL 7403633A NL 7403633 A NL7403633 A NL 7403633A NL 7403633 A NL7403633 A NL 7403633A NL 7403633 A NL7403633 A NL 7403633A
Authority
NL
Netherlands
Prior art keywords
serum
blank
triglycerides
sample
pyruvate
Prior art date
Application number
NL7403633A
Other languages
Dutch (nl)
Original Assignee
Technicon Instr B V
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Technicon Instr B V filed Critical Technicon Instr B V
Priority to NL7403633A priority Critical patent/NL7403633A/en
Publication of NL7403633A publication Critical patent/NL7403633A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/61—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving triglycerides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The automatic determination of triglycerides in serum is carrid out by (a) dividing the serum into a sample and a blank; (b) subjecting both to enzymatic hydrolysis; (c) adding the reagents necessary for converting the hydrolysis product (glycerol) into pyruvate (including a light-absorbing substance which reacts with pyruvate) to both the sample and the blank, except that the reagent which converts the glycerol into the next reaction intermediate is omitted in the blank; and (d) carrying out photometric measurements simultaneously on the sample and blank after a suitable reaction time. The use of a blank overcomes the problems associated with the fact that serum already contains pyruvate and substances which absorb at the relevant wavelength (including triglycerides.).
NL7403633A 1974-03-19 1974-03-19 Automatic determination of triglycerides in serum - using a blank comprising serum contg. hydrolysed triglycerides NL7403633A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
NL7403633A NL7403633A (en) 1974-03-19 1974-03-19 Automatic determination of triglycerides in serum - using a blank comprising serum contg. hydrolysed triglycerides

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
NL7403633A NL7403633A (en) 1974-03-19 1974-03-19 Automatic determination of triglycerides in serum - using a blank comprising serum contg. hydrolysed triglycerides

Publications (1)

Publication Number Publication Date
NL7403633A true NL7403633A (en) 1975-09-23

Family

ID=19820989

Family Applications (1)

Application Number Title Priority Date Filing Date
NL7403633A NL7403633A (en) 1974-03-19 1974-03-19 Automatic determination of triglycerides in serum - using a blank comprising serum contg. hydrolysed triglycerides

Country Status (1)

Country Link
NL (1) NL7403633A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5082769A (en) * 1988-04-15 1992-01-21 Iatron Laboratories, Inc. Aqueous solution of triglyceride substrate for determination of lipase

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5082769A (en) * 1988-04-15 1992-01-21 Iatron Laboratories, Inc. Aqueous solution of triglyceride substrate for determination of lipase

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