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MXPA05005777A - Metodo novedoso para produccion de acidos grasos poliinsaturados. - Google Patents

Metodo novedoso para produccion de acidos grasos poliinsaturados.

Info

Publication number
MXPA05005777A
MXPA05005777A MXPA05005777A MXPA05005777A MXPA05005777A MX PA05005777 A MXPA05005777 A MX PA05005777A MX PA05005777 A MXPA05005777 A MX PA05005777A MX PA05005777 A MXPA05005777 A MX PA05005777A MX PA05005777 A MXPA05005777 A MX PA05005777A
Authority
MX
Mexico
Prior art keywords
plasma
assisted sintering
sintering
assisted
sinter
Prior art date
Application number
MXPA05005777A
Other languages
English (en)
Inventor
Ulrich Zaehringer
Original Assignee
Univ Bristol
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB0229578.0A external-priority patent/GB0229578D0/en
Priority claimed from GB0316989A external-priority patent/GB0316989D0/en
Application filed by Univ Bristol filed Critical Univ Bristol
Publication of MXPA05005777A publication Critical patent/MXPA05005777A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083—Miscellaneous (1.14.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6432—Eicosapentaenoic acids [EPA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6434—Docosahexenoic acids [DHA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
  • Fats And Perfumes (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Saccharide Compounds (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)

Abstract

La presente invencion se refiere a un procedimiento mejorado para la produccion especifica de acidos grasos poliinsaturados (-3 y (-6 y un procedimiento para produccion de trigliceridos con un contenido incrementado de acidos grasos no saturados, en particular acidos grasos (-3 y (-6 que tienen por lo menos dos enlaces dobles y una longitud de cadena de 20 o 22 atomos de carbono. La invencion se refiere a la produccion de un organismo transgenico, preferentemente una planta transgenica o un microorganismo transgenico, que posee un contenido incrementado de acidos grasos, aceites o lipidos que contienen acidos grasos C20- o C22- con un enlace doble (5, 7, 8, 10, respectivamente debido a la expresion de una (8-desaturasa y una (9-elongasa de organismos tales como plantas preferentemente algas como Isochrysis galbana o Euglena gracilis. Ademas la invencion se refiere a un procedimiento para la produccion de acidos grasos poliinsaturados como acido Eicosapentanoico, Araquidonico, Docosapentanoico o Docosahexanoico a traves de la coexpresion de una (-8-desaturasa, una (-9-elongasa y una (-5 desaturasa en organismos tales como microorganismos o plantas. La invencion se refiere adicionalmente a la utilizacion de secuencias de acido nucleico especificas codificando proteinas con actividad (-8-desaturasa, 9-(-elongasa o (-5 desaturasa, constructos de acido nucleico, vectores y organismos conteniendo dichas secuencias de acido nucleico. La invencion se refiere ademas a los acidos grasos no saturados y trigliceridos que tienen un contenido incrementado de por lo menos 1% en peso de acidos grasos no saturados y la utilizacion de los mismos.
MXPA05005777A 2002-12-19 2003-12-11 Metodo novedoso para produccion de acidos grasos poliinsaturados. MXPA05005777A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB0229578.0A GB0229578D0 (en) 2002-12-19 2002-12-19 Novel method for the production of polyunsaturated fatty acids
GB0316989A GB0316989D0 (en) 2003-07-21 2003-07-21 Novel method for the production of polyunsaturated fatty acids
PCT/EP2003/014054 WO2004057001A2 (en) 2002-12-19 2003-12-11 Method for the production of polyunsaturated fatty acids

Publications (1)

Publication Number Publication Date
MXPA05005777A true MXPA05005777A (es) 2006-03-08

Family

ID=32683975

Family Applications (1)

Application Number Title Priority Date Filing Date
MXPA05005777A MXPA05005777A (es) 2002-12-19 2003-12-11 Metodo novedoso para produccion de acidos grasos poliinsaturados.

Country Status (12)

Country Link
US (1) US7714185B2 (es)
EP (1) EP1576166B1 (es)
JP (1) JP4781817B2 (es)
AR (1) AR042538A1 (es)
AU (1) AU2003296638B2 (es)
BR (1) BR0317304A (es)
CA (1) CA2510462A1 (es)
CL (1) CL2003002667A1 (es)
ES (1) ES2525212T3 (es)
IL (2) IL168783A (es)
MX (1) MXPA05005777A (es)
WO (1) WO2004057001A2 (es)

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IL218358A0 (en) 2012-04-30
AU2003296638A1 (en) 2004-07-14
EP1576166A2 (en) 2005-09-21
AU2003296638B2 (en) 2009-06-11
EP1576166B1 (en) 2014-10-15
CL2003002667A1 (es) 2005-04-08
ES2525212T3 (es) 2014-12-19
JP2006511233A (ja) 2006-04-06
JP4781817B2 (ja) 2011-09-28
BR0317304A (pt) 2005-11-08
CA2510462A1 (en) 2004-07-08
WO2004057001A3 (en) 2004-11-04
IL168783A (en) 2012-04-30
US20060246556A1 (en) 2006-11-02

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