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MXPA02002163A - Polimerizacion de acido nucleico dependiente de molde usando bloques de construccion de oligonucleotidos trifosfato. - Google Patents

Polimerizacion de acido nucleico dependiente de molde usando bloques de construccion de oligonucleotidos trifosfato.

Info

Publication number
MXPA02002163A
MXPA02002163A MXPA02002163A MXPA02002163A MXPA02002163A MX PA02002163 A MXPA02002163 A MX PA02002163A MX PA02002163 A MXPA02002163 A MX PA02002163A MX PA02002163 A MXPA02002163 A MX PA02002163A MX PA02002163 A MXPA02002163 A MX PA02002163A
Authority
MX
Mexico
Prior art keywords
template
nucleic acid
building blocks
acid polymerization
dependent nucleic
Prior art date
Application number
MXPA02002163A
Other languages
English (en)
Inventor
Kless Hadar
Original Assignee
Yeda Res & Dev
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Yeda Res & Dev filed Critical Yeda Res & Dev
Publication of MXPA02002163A publication Critical patent/MXPA02002163A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

El uso novedoso de una polimerasa dependiente a templado. El uso novedoso se efectua por emplear la polimerasa dependiente a templado para incorporar por lo menos un oligonucleotido trifosfato en un oligonucleotido-3°- OH naciente en una forma dependiente a templado.
MXPA02002163A 1999-09-01 2000-08-29 Polimerizacion de acido nucleico dependiente de molde usando bloques de construccion de oligonucleotidos trifosfato. MXPA02002163A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US38777799A 1999-09-01 1999-09-01
PCT/IL2000/000515 WO2001016366A2 (en) 1999-09-01 2000-08-29 Template-dependent nucleic acid polymerization using oligonucleotide triphosphates building blocks

Publications (1)

Publication Number Publication Date
MXPA02002163A true MXPA02002163A (es) 2004-04-21

Family

ID=23531343

Family Applications (1)

Application Number Title Priority Date Filing Date
MXPA02002163A MXPA02002163A (es) 1999-09-01 2000-08-29 Polimerizacion de acido nucleico dependiente de molde usando bloques de construccion de oligonucleotidos trifosfato.

Country Status (9)

Country Link
US (1) US7060440B1 (es)
EP (1) EP1208236A2 (es)
JP (1) JP2003508063A (es)
KR (1) KR20020047136A (es)
AU (1) AU773447B2 (es)
CA (1) CA2382657A1 (es)
IL (1) IL148344A0 (es)
MX (1) MXPA02002163A (es)
WO (1) WO2001016366A2 (es)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050287592A1 (en) * 2000-08-29 2005-12-29 Yeda Research And Development Co. Ltd. Template-dependent nucleic acid polymerization using oligonucleotide triphosphates building blocks
EP2305808B1 (en) 2001-06-20 2016-03-02 Nuevolution A/S Templated molecules and methods for using such molecules
CA2562557A1 (en) 2004-04-09 2005-10-27 The Trustees Of Boston University Method for de novo detection of sequences in nucleic acids:target sequencing by fragmentation
US7772390B1 (en) 2006-07-18 2010-08-10 The Regents Of The University Of California Lipid mediated nucleic acid synthesis
JP5745842B2 (ja) 2007-06-19 2015-07-08 ストラトス ゲノミクス インコーポレイテッド 拡張によるハイスループット核酸配列決定
US8017338B2 (en) 2007-11-20 2011-09-13 Life Technologies Corporation Reversible di-nucleotide terminator sequencing
WO2009067632A1 (en) * 2007-11-20 2009-05-28 Applied Biosystems Inc. Method of sequencing nucleic acids using elaborated nucleotide phosphorothiolate compounds
JP2012502040A (ja) * 2008-09-02 2012-01-26 アルニラム ファーマスーティカルズ インコーポレイテッド 三リン酸塩オリゴヌクレオチドの合成方法および誘導体
EP2987864B1 (en) 2009-01-29 2018-04-25 Stratos Genomics Inc. Method for detecting an analyte
US9778188B2 (en) * 2009-03-11 2017-10-03 Industrial Technology Research Institute Apparatus and method for detection and discrimination molecular object
US9482615B2 (en) 2010-03-15 2016-11-01 Industrial Technology Research Institute Single-molecule detection system and methods
US9670243B2 (en) 2010-06-02 2017-06-06 Industrial Technology Research Institute Compositions and methods for sequencing nucleic acids
US8865077B2 (en) 2010-06-11 2014-10-21 Industrial Technology Research Institute Apparatus for single-molecule detection
US8865078B2 (en) 2010-06-11 2014-10-21 Industrial Technology Research Institute Apparatus for single-molecule detection
DE102012008759A1 (de) * 2011-05-04 2012-11-08 Genovoxx Gmbh Nukleosid-Triphosphat-Konjugate und Methoden zu deren Anwendung
GB201801768D0 (en) 2018-02-02 2018-03-21 Oxford Nanopore Tech Ltd Synthesis method
TWI732405B (zh) * 2019-12-30 2021-07-01 源點生物科技股份有限公司 使用酶製備核酸序列的方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5436143A (en) 1992-12-23 1995-07-25 Hyman; Edward D. Method for enzymatic synthesis of oligonucleotides
US5714320A (en) * 1993-04-15 1998-02-03 University Of Rochester Rolling circle synthesis of oligonucleotides and amplification of select randomized circular oligonucleotides
GB9507238D0 (en) * 1995-04-07 1995-05-31 Isis Innovation Detecting dna sequence variations
US6395524B2 (en) 1996-11-27 2002-05-28 University Of Washington Thermostable polymerases having altered fidelity and method of identifying and using same

Also Published As

Publication number Publication date
AU6722900A (en) 2001-03-26
KR20020047136A (ko) 2002-06-21
US7060440B1 (en) 2006-06-13
WO2001016366A3 (en) 2002-01-17
JP2003508063A (ja) 2003-03-04
EP1208236A2 (en) 2002-05-29
CA2382657A1 (en) 2001-03-08
AU773447B2 (en) 2004-05-27
IL148344A0 (en) 2002-09-12
WO2001016366A2 (en) 2001-03-08

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