MXPA97000878A - Cck antibodies employed to improve food efficiency - Google Patents

Abstract

The present invention relates to a method for increasing feed efficiency, both in birds and mammals by using antibodies to intestine peptides, such as coleocystokinin to produce a biological response that decreases gastrointestinal mobility, reduces satiety and improves the efficiency of food

Description

CCK ANTIBODIES EMPLOYED TO IMPROVE FOOD EFFICIENCY Field of the Invention This invention relates to producing biological response in mammals or birds, either by passive transfer of an antibody or by feeding a substance containing antibody to the animal. Specifically, this invention relates to increasing feed efficiency, decreasing gastrointestinal mobility and decreasing satiety in animals and humans by the use of antibodies to coleocistokinin (CCK). Background of the Invention The immune system, based on several types of blood leukocyte cells, is a highly specific defense system that recognizes, removes and remembers foreign cells and macromolecules. While it works properly, it can distinguish between "own" and "non-owned" materials (strangers). For example, he sees tumor cells as not his own and therefore attacks them, protecting animals against tumor cells that cause cancer as it protects against other invasive macromolecules. An antigen is a foreign substance that when introduced to an animal with a functional immune system, can produce a specific immune response such as that mentioned above. Once activated, the immune response involves, among other things, production of antibodies in the circulation system specific to that antigen. There are five different classes of antibodies that are also referred to as unoglobulin. The most abundant is IgG. The other four are IgM, IgA, IgD and IgE. These antibodies combine with the antigen and act to neutralize or counteract the effects of the antigen introduced into the animal. They achieve this result by binding the antigen neutralizing it in this way and preventing it from binding to other specific cell receptors. The immune system can be used not only to combat pathogenic antigens or harmful foreign molecules, but can be manipulated in order to produce favorable responses that are not of natural origin. For example, proteins of natural origin in an animal can be neutralized by the introduction of specific antibodies to that protein thereby neutralizing the normal physiological effect of prstein in the animal's system. There are several ways in which an animal becomes unresponsive. For example, some antibodies are able to travel the placenta from the mother's circulation to that of her fetus. As a result, the progeny of that mother receive natural immune protection by "inheriting" the mother's own antibodies before birth. A second way to produce an immune response is through the introduction of an antibody to an animal, resulting in that animal developing antibodies specific to that antigen. These antibodies can be isolated from the animal and introduced into a second animal, resulting in the second animal having an antibody that can bind to the specific antigen. SUMMARY OF THE INVENTION This invention relates to producing an immune response in animals and humans, in order to increase the efficiency of food. The antibody employed in this invention is an antibody specific to the coleocystokinin peptide (CCK). The antibody coleocistoquinina (Antibody CCK) when introducing the animal, causes an increased efficiency of converting food to gain body weight and through an apparent decreased gastrointestinal mobility thereby increasing the efficiency of the food. The CCK peptide is as follows: Asp-Tyr-Met-Gly-Trp-Met-Asp-Phe-NH2 I S03H The CCK peptide can also be in a non-amide form: Asp-Tyr-Met-Gly-Trp-Met- Asp-Phe I S03H CCK is an octapeptide that has been shown to negatively affect food absorption and thus inhibits growth in both mammals (Gibbs et al., 1973) and birds (Savory and Hodgkiss, 1984). CCK antibodies have been successfully endogenously produced in pigs (Pekas and Trout, 1990, Pekas 1991) and rats (MacLaughlin et al., 1985). In both species the adverse effects of CCK on feed absorption and weight gain were avoided. by endogenous circulation of CCK antibody. The effect CCK is on domestic poultry is well known (Savory et al., 1981). CCK represents a hormone ) .. polypeptide that is released when food enters the small intestine. The presence of CCK in the intestinal mucosa alters gastrointestinal mobility (Gl). The gizzard controls the rate at which it travels through the intestine and CCK, which normally breaks off after a food is consumed, causing a decrease in gizzard contraction and an increase in intestinal contraction. This results in less time for the abosrción of food and nutrients in the intestinal tract. The inventors have found that transferring CCK antibodies in birds increases the efficiency of power. In other words, birds gain more weight per kilo of feed. The CCK presence also alters the willingness to eat. CCK is responsible for what is known as the satiety effect, which is a physiological effect that decreases markedly the appetite of a bird. If an antibody is combined with CCK, CCK is neutralized, the satiety effect is inhibited and the adverse effects of endogenous CCK in the gastrointestinal modality are avoided. In this way, the bird achieves more weight per unit of absorption. It has not been previously seen that CCK antibodies work in birds or function orally and in fact are able to neutralize the negative effects of CCK. Neuropeptide Y and bombesin have physiological effects similar to CCK in mammalian systems and bird systems. These neurotejidoe are also found in the intestine and alter feeding behavior. The effect of CCK antibodies on food efficiency and killing gain can be achieved by (1) passively transferring the CCK antibodies from the mother to the progeny, for example by injecting the producer chicken such that the progeny have increased antibody levels of CCK antibodies; (2) feeding a yolk product with a high content of CCK antibodies directly to the animal or (3) injecting a substance with a high content of CCK antibodies directly into the animal. The method in which an immune response is achieved passively involves inoculating a female bird with a specific antigen that results in passively transferring the antibody to the progeny of the female. This passive transfer of antibodies to CCK from the mother to the progeny resulting in improved conversion of feed to body weight has not been previously seen in the art.

This invention also relates to a substance containing specific antibody produced from the egg of a hen immunized against a selected antigen, wherein the substance is mixed with the food and subsequently fed to birds to produce altered but improved physiological response. CCK antibodies can be produced in laying hens, passed to the yolk, harvested from the yolk or fed as dry or dehydrated yolk, and used as a feed additive to improve feeding efficiency in birds, which has not been previously appreciated in the art previous. This invention has many advantages. An advantage is that individuals in the commercial meat industry can achieve market weight in livestock or poultry using less time and less feed, drastically reducing costs. A second advantage of the present invention is that the CCK antibodies, neutralize CCK but have no known harmful side effects and do not appear to affect the quality of the meat. Also, the cost of using this invention, even a large scale, is relatively low, since only the egg containing CCK antibody is required per 3.63 kg (8 pounds) of feed. In addition, using the method of feeding the antibody to domesticated animals, it is relatively low in labor cost, since the antibody can simply be mixed with feed and thus not each individual animal must be injected with the antibody. There is also no need to separate or isolate the antibody from the whole egg or yolk, it can simply be spray dried and fed directly. An advantage of this invention is that it counteracts the negative effect of feeding raw soybean meal to birds or livestock. For example, a typical hen diet contains 40% soybean meal, however, raw soybean meal can not feed birds because it contains trypsin inhibitor that inhibits the ability of trypsin to digest the protein. Therefore, raw soybean meal causes increased levels of CCK with concurrent decrease in feed efficiency. In order to counterattack this effect, the soybean must be heat-treated in order to feed the birds. The typical process for Preparing soybeans involves heating the grains, extracting the oils and using the remaining flour in poultry feed. Specifically, the grains should be heated to at least 121 ° C for about 20 to 40 mins. There are several problems associated with preparing soybeans for feeding birds, one is that the The heating process must be carried out at an extremely high temperature to ensure destruction of the trypsin inhibition factor. Second, heating has a negative impact on protein quality in soybean meal and makes the denatured protein difficult to digest appropriately. However, the inventors have found that CCK antibodies protect against the negative effects of feeding raw soy to birds. In addition to soy, there are a number of other plants that contain trypsin inhibitor including wheat, barley, beans and various legumes. It is predicted that the CCK antibody will also protect against the negative effects of feeding products made from wheat, barley, beans or legumes to birds or livestock. This invention also has many advantages over what is currently used in the poultry and livestock industries. Antibiotics are currently used in the commercial animal industry to increase food efficiency and weight gain. However, antibiotics leave a drug residue in the animal's tissue. Therefore, the animal must go through a "withdrawal or separation time". The withdrawal time is a sufficient amount of time for the antibiotic to leave the animal's tissues. During the withdrawal time, the animal can not be slaughtered for human consumption. Additionally, any eggs or milk produced can not be used for human use. This precaution is used due to the concern that the human consumption of milk with traces of penicillin, for example, will cause increased resistance to antibiotics in humans, especially making the use of antibiotics useless to fight against bacterial diseases.

Second, the use of antibiotics over a long period of time can potentially cause an increased number of microorganisms capable of infecting an animal, because these organisms slowly gain resistance due to constant exposure to the antibiotic. In this way, future bacterial diseases will be difficult if not impossible to tare. CCK also has the same effects of increasing mobility Gl and inhibition of satiety in mammals (Pekas and Trout, 1990). It is a well-known fact that mammalian species passively pass antibodies to their progeny like birds and that mammals respond to CCK autoimmunization like birds. The mother antibodies are also identical to those passively transferred to the progeny in birds as well as in mammals. Similarly, feeding raw soy exerts analogous increases in CCK production in mammals as well as in birds (Weller et al., 1990; Chohen et al., 1993; Can J An Sci 73; 401). Therefore, based on the aforementioned facts, the protective effects of CCK antibodies actively supplied and passively transferred against satiety and deficient feed conversion resulting from CCK observed in birds, would also be seen in mammals. Using CCK in various livestock such as swine and cattle, their final weight will be drastically increased using the same amount of animal feed. In this way, the costs of producing an animal for market size are reduced and this would have an enormously beneficial effect on the livestock industry. The invention would be highly beneficial for humans who are underweight or have problems in maintaining their weight. Additionally, individuals with eating disorders will benefit from this invention because their food absorption will be controlled. As previously established, there are other peptides or gastrointestinal hormones that have an effect on the behavior and digestion of feeding of the animal. The effect of CCK and the method of using CCK antibodies directly towards that peptide in order to avoid adverse effects CCK, suggests that similar responses can be achieved using other antibodies specific to peptides or gastrointestinal hormones. For example, gastrin is involved in signaling the secretion of acid in the intestines and has a trophic action in the gastric mucosa leading to interplasia. An antibody to gastrin can be used to decrease secretion of acid in animals with gastric ulcers or in cases of carcinoid tumors of gastric ELC cells due to prolonged hypergastrinemia. Intestinal somatostatin inhibits absorption of food when feeding animals, as well as many other activities of the intestine. An antibody to somatostatin can prevent its inhibitory activities. Bombesin stimulates a release of CCK. It can be assumed that inhibiting bombesin using bombesin-specific antibody can result in similar responses to CCK-specific antibodies. Neuropeptide Y has been reported as a stimulus in food. It may be possible to inhibit their irregular activity to obesity in animals tending to develop these problems. The biological activity of other peptides that regulate intestinal mobility and other functional properties of the intestine can be regulated using the described technology. In general, by generating antibodies to peptides, hormones, cytokines, etc. that regulate metabolic, physiological and / or behavioral biochemical processes, it may be possible to regulate or alter an animal's system by compensating for a physical abnormality or accentuating a function. normal. Detailed Description of the Preferred Modality As previously mentioned, there are three modes of producing an immune response to CCK in mammals or birds: passive transfer, active feeding, and active inoculation. The mode of this invention that relates to passively transferring antibodies involves injecting laying hens with CCK, where the hens produce specific CCK antibodies and as a result, these antibodies are then transferred to the egg yolk, from eggs laid by the hens. The embryo of the hen absorbs the CCK antibody during embryonic development. Subsequently, the CCK antibodies circulate in the bloodstream of the incubated chicken, as well as passing into the gastrointestinal tract. Either purified CCK or synthesized CCK peptide can be filled. Means well known in the art can be used to purify the CCK peptide such as fractionation, chromatography, precipitation or extraction. However, CCK must be conjugated with a foreign carrier or protein to be used as the antigen. CCK only has a molecular weight less than 1500 Daltons. In order to invoke an immune response, a molecular weight of at least 10,000 Daltons is required. Therefore the CCK peptide should be conjugated to a carrier protein with a molecular weight of about 8,000 Daltons or more, in order for the conjugate to produce an immune response. Carriers include a wide variety of conventionally known substances but commonly involve gamma globulin or keyhole limpet hemocyanin. The CCK peptide conjugated to its carrier protein is injected into the target animal with a common adjuvant. The CCK carrier conjugate can be emulsified in complete Freund's adjuvant, for example. If the mammals are the target animals, then subsequent inoculations must consist of incomplete adjuvant. Another mode of this invention involves orally feeding a substance containing CCK antibody produced from eggs of a chicken vaccinated with CCK. The eggs containing CCK antibodies are prepared and mixed in animal feed. Birds or mammals that consume this food containing antibody, will soon show beneficial response by avoiding satiety effects specific to CCK. The production of CCK antibodies for oral administration can be carried out by using known technology to produce antibodies in egg yolk. In this process, hens are subjected to injecting them with CCK conjugated to a carrier protein. In response to exposure to the CCK antigen, the eggs laid by these hens contain high levels of CCK antibodies in the yolk. New automated systems separate and spray the yolks in a powder. The yolks can alternatively be reused. That standard technique is well established in the art to produce various antibodies for other purposes, (eg, diagnosis, resistance to pathogens, etc.). Whole eggs can be used and to date it is not necessary to separate the yolk from the albumin. Typically, eggs containing .l to CCK are used per 3.63 kg. (8 pounds) of food. Chickens are the most preferable source of eggs, but turkeys, geese, ducks and the like may also be used.

While eggs are the logical source of massive amounts of antibodies, it is possible to collect antibodies from whole blood, plasma or serum when hens are processed for meat. In addition, whole blood plasma or inoculated cattle serum can be another source of antibodies as well as milk derived from an inoculated cow or goat. Additionally, another source of antibody production is through cell fusion using hybridoma techniques, genetically altered cell cultures or fermentation using recombinant technology. A third mode of this invention is by inoculation. CCK antibodies can be injected directly into a target animal in order to produce the desired satiety response and improved feed conversion. The target animal that receives the CCK antibody varies greatly. Commercial animals such as cattle, birds and fur animals, (for example mink, marten cibelina, etc.) are ideal candidates. Additionally, humans who have difficulty in gaining weight are also considered to be within the scope of this invention. CCK ANTIBODIES TRANSFERRED PASSIVELY IN PERFORMANCE OF LEGHORN POLLITES EXAMPLE X Methods Coleocistokinin (CCK-8) (Fragment 26-33 amide with tyrosine sulphated) is conjugated to keyhole limpet hemocyanin (KLH) using glutaraldehyde and emulsified with complete Freund's adjuvant (1 : 1) and injected (100 ug CCK) in 11 simple White Comb 5 leghorn laying hens. A second injection of the CCK-8 conjugate in incomplete Freund's adjuvant is injected 7 days after the primary injection. Another group of control hens that does not receive the CCK injection was also tested. Hens (control and injected with CCK) were fertilized (artificially using &jt semen collected from New Hampshire roosters). Fertile eggs collected 5 months after the initial injection were used to determine the performance of the hen as a result of passively transferred CCK antibodies. Fifteen incubated chicks from the control chickens and fifteen incubated chicks 5 from the chickens injected with CCK were developed into battery-based producers based on corn-soybean meal diets for six weeks. The data on corporate weight gain and food consumption were collected. Results 0 Chicks of the hens injected with CCK had improved feed conversion (less feed per kilo of gain) which can be 14% better than chicks of the control hens. Also, food absorption is increased in CCK birds. The results are illustrated in Table 1. 5 TABLE I Treatment Gain in% Absorption% Antibody 6 weeks * Change in 6 weeks * Change Control 297 745 CCK 352 +18 756 +1 (Continued Table I) Treatment Conversion% Antibody 6 weeks Change Control 2.51 CCK 2.15 -14 * Body weight, body weight gain and food absorption are measured in grams. EXAMPLE 2 Methods Eggs from hens immunized with CCK (as illustrated in Example 1) and from control hens were harvested approximately 10 months after primary inoculation. Two pens of 13 chicks (representing both control hens and those immunized with CCK) were fed a diet based on corn-soybean meal to determine if CCK antibodies transferred actively influence performance as seen in example 1. Birds were developed for 4 weeks. Body weights and feed intake are determined.

Results Food conversion was improved by 2% in chickens from chickens immunized with CCK when compared to control chickens. The results are illustrated in Table II. TABLE JLI Treatment Gain in% Absorption% of 4 weeks * Change in 4 weeks * Change Control 158 383 CCK 151 -4 360 -6 (Continued Table II) Treatment Conversion% of 4 weeks Change Control 2.42 CCK 2.38 -2 * Body weight, body weight gain and absorption of food are measured in grams. Example 3 Methods Fertile eggs were harvested approximately 8 months after primary inoculation of the control hens and injected with CCK (immunization as described in Example 1) and amplified to study the effects of CCK immunization on progeny performance. Two corrals of 17 progeny chickens by chickens of chickens injected with CCK and 2 corrals of 17 chickens of progeny by control chickens, they were developed for 4 weeks. Body weight and food consumption were measured. Results Chickens from hens injected with CCK had a 5.2% improvement in feed conversion than chickens from control hens. The results are illustrated in Table III. TABLE III Treatment% Gain in Absorption%% of 4 weeks * Change in 4 weeks * Change Control 246 473 CCK 245 0 447 -5.5 (Continued Table III) Treatment Conversion% of 4 weeks Change Control 1.92 CCK 1.82 -5.2 * Body weight, body weight gain and absorption of food are measured in grams. Example 4 Methods In this study, 2 Corrales of 15 chickens per corrals of hens immunized with CCK (as illustrated in Example 1 and 7 months after the primary inoculation of the hen) and 2 pens of 12 chickens per pen of chickens of Control was developed on a diet based on corn-soybean meal supplemented with 5% raw soy for three weeks (raw soybeans are used to stimulate the production of CCK). Body weight and feed intake were measured. Results Chicken chickens injected with CCK had a 10% improvement in feed conversion when compared to control chickens. The results are illustrated in Table IV. TABLE IV Treatment Gain in% Absorption% of 3 weeks * Change in 3 weeks * Change Control 169 395 CCK 161 -5 338 -14 (Continued Table IV) Treatment Conversion% of 3 weeks Change Control 2.34 CCK 2.10 -10 * Body weight, body weight gain and absorption of food are measured in grams. PROTOCOL FOR THE EFFECTS OF CCK ANTIBODIES TRANSFERRED PASSIVELY, IN THE PERFORMANCE OF YOUNG BROILER CHICKENS Example 5 Methods Producers were immunized with CCK conjugated to KLH using the protocol described in example 1. Since these producers are kept on the floor, the fertile eggs they were produced as a result of natural couplings. A total of 10 chickens that received CCK immunization (antigen prepared as Example 1 for Leghorns), and 10 chickens served as 5 controls. Approximately 21 to 30 days after the primary inoculation, fertile eggs were collected from the control hens and immunized with CCK. Seven broiler chickens from the control hens and 7 broiler chickens from the CCK injected hens were incubated and developed in a battery-operated producer for 3 weeks. Body weight and food consumption were measured. Results Food conversion was improved by 20% and body weight by 8% in broiler chickens from CCK-immunized hens compared to broiler chicken from control hens. See Table V for results. TABLE V Treatment Gain in% Absorption% of 3 weeks * Change in 3 weeks * Change 20 C Coonnttrrooll 3 39966 604 CCK 427 +8 526 -13 (Continued Table V) Treatment Conversion% of 3 weeks Change Control 1.53 CCK 1.23 -20 * Body weight, body weight gain and absorption of food are measured in grams. EXAMPLE 6 Methods Two pens of 6 chicken broiler producers immunized with CCK, 7 weeks after primary inoculation as in Example 5, and 2 pens of 6 chickens per pen of the control hens were incubated and developed at 3 weeks of age in a standard broiler type diet. Body weight and food consumption were measured. Results Broiler chickens from chickens immunized with CCK achieved 16% more body weight and converted food 12.5% more efficiently than chickens from the control hens. See Table VI for results. TABLE VI Work Gain in% Absorption% of 3 weeks ^ * Change in 3 weeks * Change Control 380 547 CCK 441 +16 547 0 (Continued Table VI) Treatment Conversion% of 3 weeks C child Control 1.44 CCK 1.26 -12.5 * Weight body weight, body weight gain and food absorption are measured in grams. FEEDING AN EGG YEM OF CONTROL HENS AND IMMUNIZED WITH CCK Example 7 Methods Control of immunized hens CCK was perparated as described in example 1. Control chickens eggs immunized CCK were collected after at least 21 days after primary inoculation . Egg yolks were collected (albumin was discarded) and control and anti-CCK buds were separately collected, frozen and then freeze dried. The dried control yolks and CCK antibodies were then ground and added to a standard corn-soybean diet at .5, 1.0 or 5% of the diet (weight by weight) creating 3 control treatments and 3 anti-CCK treatments. Each diet treatment is fed to two pens of 9 leghorn chickens for 4 weeks. The gains in body weight, feed intake and feed conversion were determined.

Results As the level of anti-CCK egg yolk increased, body weight gain increased with respect to those fed the control yolk. At each feeding level with egg yolk with anti-CCK, food conversion was improved over those fed to the control yolk. See Table VII for results. TABLE VII 0 to 4 weeks of age C; Treatment% Feeding Absorption Conversion of food * of food * Control bud .5 692 2.88 Bud CCK .5 680 2.50 Control bud 1.0 656 2.39 15 Bud CCK 1.0 649 2.29 Control bud 5 712 2.55 Bud CCK 5 772 2.49 @ * Weight body weight, body weight gain and food absorption are measured in grams. EFFECTS OF PASSIVELY TRANSFERRING CCK ANTIBODIES TO AVOID THE NEGATIVE EFFECTS OF FEEDING RAW SOY IN FOOD CONVERSION Example 8 Methods Immunized hens (Leghorns) were prepared as in Example 1. The hens were artificially fertilized and the eggs were collected and incubated. Chickens (Single Comb White Leghorn X New Hampshire) were incubated and 2 pens of 12 chickens were assigned to each of 4 treatments. The treatments included 2 chicken sources (progeny of the control hens and immunized with CCK) arranged factorially with 2 dietary treatments (5 or 10% of raw soybeans at the cost of diet). The chickens were fed the diets for 4 weeks and body weight and feed intake were measured. Results Chickens from CCK immunized hens had improved feed conversion (11% to 19%) when compared to their respective control diets. As the level of raw soy increased in the diet, the feed conversion was more deficient (12% more deficient in the control progeny but only 6% more deficient in the progeny chickens of the hens injected with CCK). See Table VIII for results. TABLE VIII% in Soy Antibody Weight 4% of Raw CCK Passive Weeks * Change 5 - 202 5 + 205 +1.5 10 - 192 10 + 197 +2.6% in Soybean Conversion% of Raw 4 weeks Change 5 2.63 5 2.34 -11 10 2.94 10 2.48 -19 * Body weight, body weight gain and food absorption are measured in grams.

Claims (54)

1. CLAIMS 1. A method for transferring coleocistokinin antibodies (CCK) to an animal in order to increase feed efficiency and decrease intestinal mobility: administer to the animal a substance containing CCK antibody wherein a substance is derived from a producer animal, wherein the producing animal has been immunized with a coleocistokinin peptide.
2. 2. The method according to claim 1, characterized in that the administration is by inoculation.
3. 3. The method according to claim 1, characterized in that the administration is by feeding.
4. 4. The method according to claim 1, characterized in that the animal is a bird.
5. 5. The method according to claim 4, characterized in that the bird is a domesticated bird.
6. 6. The method according to claim 1, characterized in that the animal is a mammal.
7. 7. The method according to claim 1, characterized in that the producing animal is chosen from porcine, bovine, wool or goat.
8. The method according to claim 1, characterized in that the substance is derived from a by-product of the producing animal.
9. 9. The method according to claim 8, characterized in that the by-product is chosen from whole blood, serum, plasma or milk.
10. 10. The method according to claim 1, characterized in that the producing animal is a female bird.
11. 11. The method according to claim 10, characterized in that the female bird is a domesticated bird.
12. The method according to claim 10, characterized in that the substance is derived from whole egg yolk.
13. 13. The method according to claim 10, characterized in that the substance is derived from egg yolk.
14. The method according to claim 1, characterized in that the CCK is purified CCK peptide.
15. 15. The method according to claim 1, characterized in that the CCK is synthetic CCK peptide.
16. 16. The method according to claim 1, characterized in that the CCK is sulfated.
17. 17. The method according to claim 1, characterized in that the CCK is not sulfated.
18. 18. The method according to claim 1, characterized in that the CCK is an amide.
19. 19. The method according to claim 1, characterized in that the CCK is conjugated to a carrier protein.
20. 20. The method according to claim 19, characterized in that the carrier protein is keyhole limpet hemocyanin.
21. 21. The method according to claim 19, characterized in that the carrier protein is bovine gammaglobulin.
22. 22. The method according to claim 19, characterized in that the CCK conjugated to the carrier protein has a molecular weight of at least 8,000 Daltons.
23. 23. The method according to claim 6, characterized in that the domesticated bird is a hen.
24. 24. The method according to claim 7, characterized in that the mammal is a rodent.
25. 25. The method according to claim 7, characterized in that the mammal is homo sapiens.
26. 26. The method according to claim 7, characterized in that the mammal is a domesticated mammal.
27. 27. The method according to claim 7, characterized in that the domesticated mammal is chosen from porcine, bovine, wool or goat.
28. 28. The method to transfer antibodies of coleocistoquinina (CCK) in animals in order to increase efficiency in food and decrease intestinal mobility: immunize an animal with CCK, so that the animal produces antibodies to CCK and passively transfer the antibodies to the progeny of the animal, with which the progeny shows an immune response of increased feed efficiency and decreased intestinal mobility.
29. 29. The method according to claim 28, characterized in that the animal is a bird.
30. 30. The method according to claim 28, characterized in that the animal is a mammal.
31. 31. The method according to claim 29, characterized in that the bird is domesticated. 1.
32. 32. The method according to claim 31, characterized in that the domesticated bird is a hen.
33. 33. The method according to claim 28, characterized in that the CCK is conjugated to a carrier protein.
34. 34. The method according to claim 33, characterized in that the carrier protein is keyhole limpet hemocyanin.
35. 35. The method according to claim 33, characterized in that the carrier protein is bovine gammaglobulin.
36. 36. The method according to claim 33, characterized in that the CCK conjugated to the carrier protein has a molecular weight of at least 8,000 Daltons.
37. 37. The method according to claim 30, characterized in that the mammal is a rodent.
38. 38. The method according to claim 30, characterized in that the mammal is homo sapiens.
39. 39. The method according to claim 30, characterized in that the mammal is a domesticated mammal.
40. 40. The method according to claim 39, characterized in that the domesticated mammal is chosen from porcine, bovine, wool or goat.
41. 41. A food substance for an animal, characterized in that it comprises: an organic food; and an I. "effective amount of antibodies binding to CCK.
42. 42. The food according to claim 41, characterized in that the antibodies are derived from eggs of immunized birds.
43. 43. The food according to claim 15 41, characterized in that the animal is a domesticated animal.
44. 44. The food according to claim 43, characterized in that the domesticated animal is a domesticated bird.
45. 45. The food according to claim 20 44, characterized in that the domesticated bird is a hen.
46. 46. The food according to claim 43, characterized in that the domesticated animal is a mammal.
47. 47. The food according to claim 46, characterized in that the mammal is chosen from porcine, bovine, 25 sheep or goats.
48. 48. The method for counteracting the negative effects of trypsin inhibitor when feeding plant material containing trypsin inhibitor to animals, characterized in that it comprises: feeding the animals with a feed wherein the 5 food contains an effective amount of CCK antibodies.
49. 49. The method according to claim 48, characterized in that the plant material is soybean.
50. 50. A method for transferring peptide antibodies from intestine to an animal that negates the physiological effect of the peptide: administering to the animal an antibody specific to an intestine peptide, when the peptide from the intestine is derived from a producer animal wherein the Producer animal has been immunized with an intestine peptide.
51. 51. The method according to claim 50, characterized in that the intestine peptide is chosen from bombesin, neuropeptide Y, gastrin or somatostatin.
52. 52. A method for transferring peptide antibodies from intestine in animals in order to increase feed efficiency and decrease satiety, characterized in that it comprises: 20 immunize an animal with a peptide from the intestine, such that the animal produces antibodies to the intestine peptide and passively transfer the antibodies to the progeny of the animal that negates the physiological effect of the peptide.
53. 53. The method according to claim 52, wherein the intestine peptide is chosen from bombesin, neuropeptide Y, gastrin or somatostatin.
54. 54. The method for transferring antibodies to an animal in order to modify a biochemical process, characterized in that it comprises: administering to the animal an antibody-containing substance wherein the substance is derived from a producing animal where the producing animal has been immunized with a antigen, where the antigen regulates a biochemical process.