MX2023001017A - Eliminacion doble de alanina racemasa y transcomplementacion. - Google Patents
Eliminacion doble de alanina racemasa y transcomplementacion.Info
- Publication number
- MX2023001017A MX2023001017A MX2023001017A MX2023001017A MX2023001017A MX 2023001017 A MX2023001017 A MX 2023001017A MX 2023001017 A MX2023001017 A MX 2023001017A MX 2023001017 A MX2023001017 A MX 2023001017A MX 2023001017 A MX2023001017 A MX 2023001017A
- Authority
- MX
- Mexico
- Prior art keywords
- alanine racemase
- present
- transcomplementation
- host cell
- bacterial host
- Prior art date
Links
- 108010041525 Alanine racemase Proteins 0.000 title abstract 4
- 230000037430 deletion Effects 0.000 title 1
- 238000012217 deletion Methods 0.000 title 1
- 230000001580 bacterial effect Effects 0.000 abstract 3
- 210000004027 cell Anatomy 0.000 abstract 3
- 230000002759 chromosomal effect Effects 0.000 abstract 2
- 102000040430 polynucleotide Human genes 0.000 abstract 2
- 108091033319 polynucleotide Proteins 0.000 abstract 2
- 239000002157 polynucleotide Substances 0.000 abstract 2
- 229920001184 polypeptide Polymers 0.000 abstract 2
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 2
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- 210000000349 chromosome Anatomy 0.000 abstract 1
- 230000014509 gene expression Effects 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 239000013612 plasmid Substances 0.000 abstract 1
- 230000010076 replication Effects 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/90—Isomerases (5.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y501/00—Racemaces and epimerases (5.1)
- C12Y501/01—Racemaces and epimerases (5.1) acting on amino acids and derivatives (5.1.1)
- C12Y501/01001—Alanine racemase (5.1.1.1)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/10—Plasmid DNA
- C12N2800/101—Plasmid DNA for bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
- C12R2001/10—Bacillus licheniformis
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Peptides Or Proteins (AREA)
Abstract
La presente invención se refiere a una célula huésped bacteriana en la que se inactivó un primer gen cromosómico que codifica una primera alanina racemasa y un segundo gen cromosómico que codifica una segunda alanina racemasa. Dicha célula huésped bacteriana comprende un plásmido que comprende al menos una secuencia de replicación autónoma o presente como múltiples copias en el cromosoma, un casete de expresión génica que comprende un polinucleótido que codifica al menos un polipéptido de interés, unido operativamente a un promotor, y un polinucleótido que codifica una tercera alanina racemasa, unida operativamente a un promotor. La presente invención se refiere además a un método para producir al menos un polipéptido de interés basado en el cultivo de la célula huésped bacteriana de la presente invención.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP20187745 | 2020-07-24 | ||
| EP20187746 | 2020-07-24 | ||
| PCT/EP2021/070696 WO2022018268A1 (en) | 2020-07-24 | 2021-07-23 | Alanine racemase double deletion and transcomplementation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX2023001017A true MX2023001017A (es) | 2023-03-01 |
Family
ID=77179989
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2023001016A MX2023001016A (es) | 2020-07-24 | 2021-07-23 | Eliminacion simple de alanina racemasa y transcomplementacion. |
| MX2023001017A MX2023001017A (es) | 2020-07-24 | 2021-07-23 | Eliminacion doble de alanina racemasa y transcomplementacion. |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2023001016A MX2023001016A (es) | 2020-07-24 | 2021-07-23 | Eliminacion simple de alanina racemasa y transcomplementacion. |
Country Status (9)
| Country | Link |
|---|---|
| US (2) | US20230295603A1 (es) |
| EP (2) | EP4185689A1 (es) |
| JP (2) | JP2023534742A (es) |
| KR (2) | KR20230041694A (es) |
| CN (1) | CN116249777A (es) |
| BR (2) | BR112023001024A2 (es) |
| CA (2) | CA3186369A1 (es) |
| MX (2) | MX2023001016A (es) |
| WO (2) | WO2022018260A1 (es) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2024096123A1 (ja) * | 2022-11-04 | 2024-05-10 | 株式会社バイオパレット | 遺伝子が改変された微生物およびその生産方法 |
| WO2024146919A1 (en) | 2023-01-05 | 2024-07-11 | Basf Se | Use of foldases to improve heterologous expression of secreted molecules |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2144990T3 (es) | 1989-08-25 | 2000-07-01 | Henkel Of America Inc | Enzima proteolitica alcalina y metodo de produccion. |
| JP3529774B2 (ja) | 1991-11-14 | 2004-05-24 | ノボザイムス アクティーゼルスカブ | バシラス・リヘニフォルミスｘ−アミラーゼのプロモーターの変異体由来のバシラス属細菌のプロモーター |
| FR2704860B1 (fr) | 1993-05-05 | 1995-07-13 | Pasteur Institut | Sequences de nucleotides du locus cryiiia pour le controle de l'expression de sequences d'adn dans un hote cellulaire. |
| US5958728A (en) | 1996-11-18 | 1999-09-28 | Novo Nordiskbiotech, Inc. | Methods for producing polypeptides in mutants of bacillus cells |
| JP2004501651A (ja) | 2000-06-23 | 2004-01-22 | ノボザイムス アクティーゼルスカブ | 遺伝子の安定した染色体多コピー組み込みのための方法 |
| US8268586B2 (en) | 2006-12-21 | 2012-09-18 | Novozymes, Inc. | Modified messenger RNA stabilizing sequences for expressing genes in bacterial cells |
| ES2570962T3 (es) | 2007-06-07 | 2016-05-23 | Dsm Ip Assets Bv | Producción incrementada de un producto diana vía estabilización del ARNm |
| KR101683302B1 (ko) | 2008-03-28 | 2016-12-06 | 다니스코 유에스 인크. | 박테리아 세포 내 로커스 증폭 방법 |
| CN102203277B (zh) * | 2008-08-21 | 2014-03-12 | 诺维信公司 | 微流体装置筛选方法 |
| WO2015055558A1 (en) | 2013-10-17 | 2015-04-23 | Dsm Ip Assets B.V. | Protein expression system |
| WO2015091280A1 (en) | 2013-12-20 | 2015-06-25 | Basf Se | A process for production of a protein of interest in a microbial host organism |
| WO2015118126A1 (en) | 2014-02-07 | 2015-08-13 | Dsm Ip Assets B.V. | Improved bacillus host |
| WO2015181296A1 (de) | 2014-05-30 | 2015-12-03 | Mahle International Gmbh | VORRICHTUNG UND VERFAHREN ZUM FASERSPRITZGIEßEN VON SPRITZGUSSTEILEN |
| ES2586979B1 (es) * | 2015-04-15 | 2017-07-25 | Fundación Profesor Novoa Santos | Vacunas vivas atenuadas de staphylococcus aureus |
| WO2018009520A1 (en) | 2016-07-06 | 2018-01-11 | Novozymes A/S | Improving a microorganism by crispr-inhibition |
| CN110892066A (zh) | 2017-07-21 | 2020-03-17 | 巴斯夫欧洲公司 | 转化细菌细胞的方法 |
-
2021
- 2021-07-23 EP EP21749571.2A patent/EP4185689A1/en active Pending
- 2021-07-23 WO PCT/EP2021/070662 patent/WO2022018260A1/en active Application Filing
- 2021-07-23 US US18/017,430 patent/US20230295603A1/en active Pending
- 2021-07-23 EP EP21749578.7A patent/EP4185690A1/en active Pending
- 2021-07-23 JP JP2023504536A patent/JP2023534742A/ja active Pending
- 2021-07-23 JP JP2023504329A patent/JP2023534719A/ja active Pending
- 2021-07-23 BR BR112023001024A patent/BR112023001024A2/pt not_active Application Discontinuation
- 2021-07-23 CA CA3186369A patent/CA3186369A1/en active Pending
- 2021-07-23 CN CN202180059890.1A patent/CN116249777A/zh active Pending
- 2021-07-23 MX MX2023001016A patent/MX2023001016A/es unknown
- 2021-07-23 KR KR1020237001807A patent/KR20230041694A/ko unknown
- 2021-07-23 KR KR1020237006115A patent/KR20230042088A/ko unknown
- 2021-07-23 US US18/017,426 patent/US20230272358A1/en active Pending
- 2021-07-23 MX MX2023001017A patent/MX2023001017A/es unknown
- 2021-07-23 CA CA3186383A patent/CA3186383A1/en active Pending
- 2021-07-23 WO PCT/EP2021/070696 patent/WO2022018268A1/en active Application Filing
- 2021-07-23 BR BR112023000958A patent/BR112023000958A2/pt not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| KR20230041694A (ko) | 2023-03-24 |
| KR20230042088A (ko) | 2023-03-27 |
| CA3186369A1 (en) | 2022-01-27 |
| WO2022018268A1 (en) | 2022-01-27 |
| JP2023534719A (ja) | 2023-08-10 |
| BR112023001024A2 (pt) | 2023-02-14 |
| EP4185689A1 (en) | 2023-05-31 |
| EP4185690A1 (en) | 2023-05-31 |
| JP2023534742A (ja) | 2023-08-10 |
| MX2023001016A (es) | 2023-03-01 |
| US20230295603A1 (en) | 2023-09-21 |
| US20230272358A1 (en) | 2023-08-31 |
| CN116249777A (zh) | 2023-06-09 |
| CA3186383A1 (en) | 2022-01-27 |
| BR112023000958A2 (pt) | 2023-02-07 |
| WO2022018260A1 (en) | 2022-01-27 |
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