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MX2021001218A - Acidos nucleicos que codifican proteinas transaminasas mejoradas. - Google Patents

Acidos nucleicos que codifican proteinas transaminasas mejoradas.

Info

Publication number
MX2021001218A
MX2021001218A MX2021001218A MX2021001218A MX2021001218A MX 2021001218 A MX2021001218 A MX 2021001218A MX 2021001218 A MX2021001218 A MX 2021001218A MX 2021001218 A MX2021001218 A MX 2021001218A MX 2021001218 A MX2021001218 A MX 2021001218A
Authority
MX
Mexico
Prior art keywords
proteins
nucleic acids
acids encoding
improved transaminase
encoding improved
Prior art date
Application number
MX2021001218A
Other languages
English (en)
Inventor
Mark James Ford
Oliver Kensch
Wayne Coco
Nikolaus Pawlowski
Christina Thies
Andreas Karl Steib
Ulrike Keller
Moritz Schön
Ksenia Niesel
Nina Bohlke
Saskia Funk
Cindy Schulenburg
Original Assignee
Bayer Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bayer Ag filed Critical Bayer Ag
Publication of MX2021001218A publication Critical patent/MX2021001218A/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1096—Transferases (2.) transferring nitrogenous groups (2.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/001—Amines; Imines
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/06—Alanine; Leucine; Isoleucine; Serine; Homoserine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/22—Tryptophan; Tyrosine; Phenylalanine; 3,4-Dihydroxyphenylalanine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P41/00—Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P9/00—Preparation of organic compounds containing a metal or atom other than H, N, C, O, S or halogen
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y206/00—Transferases transferring nitrogenous groups (2.6)
    • C12Y206/01—Transaminases (2.6.1)
    • C12Y206/01018—Beta-alanine-pyruvate transaminase (2.6.1.18)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/101—Plasmid DNA for bacteria

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

La presente invención se refiere a proteínas que poseen una actividad mejorada de omega-transaminasa (?-TA), a moléculas de ácido nucleico que codifican proteínas respectivas que tienen una actividad mejorada de ?-TA y a métodos para la síntesis estereoselectiva de aminoácidos y aminas quirales o para el aumento de isómeros de aminas quirales en mezclas de enantiómeros.
MX2021001218A 2018-07-31 2019-07-30 Acidos nucleicos que codifican proteinas transaminasas mejoradas. MX2021001218A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP18186729 2018-07-31
PCT/EP2019/070431 WO2020025577A1 (en) 2018-07-31 2019-07-30 Nucleic acids encoding improved transaminase proteins

Publications (1)

Publication Number Publication Date
MX2021001218A true MX2021001218A (es) 2021-04-12

Family

ID=63259382

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2021001218A MX2021001218A (es) 2018-07-31 2019-07-30 Acidos nucleicos que codifican proteinas transaminasas mejoradas.

Country Status (10)

Country Link
US (1) US20220220457A1 (es)
EP (1) EP3830252A1 (es)
JP (1) JP7496814B2 (es)
KR (1) KR20210038923A (es)
CN (1) CN112888780A (es)
BR (1) BR112021001800A2 (es)
IL (1) IL280400A (es)
MX (1) MX2021001218A (es)
TW (1) TWI851589B (es)
WO (1) WO2020025577A1 (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111909907B (zh) * 2020-07-08 2022-05-24 浙江工业大学 天冬氨酸氧化酶突变体、工程菌及其在氧化-还原偶联制备精草铵膦中的应用
CN114645027B (zh) * 2020-12-21 2024-12-10 上海合全药物研发有限公司 一种来源于巨大芽孢杆菌的氨基转移酶突变体及其应用
US20240182502A1 (en) 2021-04-01 2024-06-06 Basf Se Methods for preparing l-glufosinate
CN114134126B (zh) * 2021-10-28 2023-12-05 浙江大学杭州国际科创中心 转氨酶及其突变体在制备(s)-1-甲氧基-2-丙胺中的应用
CN114277011B (zh) * 2021-12-29 2024-02-06 凯莱英医药集团（天津）股份有限公司 转氨酶突变体及其应用

Family Cites Families (15)

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Publication number Priority date Publication date Assignee Title
US4950606A (en) 1989-06-22 1990-08-21 Celgene Corporation Enantiomeric enrichment and stereoselective synthesis of chiral amines
US6133018A (en) 1997-06-02 2000-10-17 Celgro Enzymatic synthesis of chiral amines using -2-amino propane as amine donor
CN1990868A (zh) * 1999-06-25 2007-07-04 Basf公司 编码参与内环境稳定和适应的蛋白质的谷氨酸棒杆菌基因
KR100433134B1 (ko) 2002-03-05 2004-05-27 김병기 신규한 호열성 미생물 및 이를 이용한 방향족ｌ-아미노산의 제조 방법
JP4125268B2 (ja) 2004-07-07 2008-07-30 株式会社島精機製作所 横編機における給糸装置のヤーンフィーダ
JP4626198B2 (ja) 2004-07-13 2011-02-02 四国化工機株式会社 容器供給装置
US7172885B2 (en) * 2004-12-10 2007-02-06 Cambrex North Brunswick, Inc. Thermostable omega-transaminases
TW200722519A (en) * 2005-12-07 2007-06-16 Cambrex North Brunswick Inc Thermostable omega-transaminases
AU2008214612B2 (en) * 2007-02-14 2013-12-19 Bayer Cropscience Aktiengesellschaft Truncated alternansucrase coding nucleic acid molecules
DE102009000592A1 (de) * 2009-02-04 2010-08-05 Evonik Degussa Gmbh Verfahren zur Herstellung von Aminogruppen tragenden, multizyklischen Ringsystemen
US9029113B2 (en) * 2011-03-11 2015-05-12 Kaneka Corporation Modified aminotransferase, gene thereof, and method for producing optically active amino compound using same
JP2016537991A (ja) * 2013-11-26 2016-12-08 ▲凱▼菜英医▲薬▼集▲団▼（天津）股▲フン▼有限公司 Ｒ型ω−トランスアミナーゼ及びその応用
BR112016029375A2 (pt) 2014-06-16 2017-10-17 Invista Tech Sarl métodos, reagentes e células para biossintetizar compostos
WO2016198660A1 (en) * 2015-06-12 2016-12-15 C-Lecta Gmbh Transaminases
CN116121315A (zh) 2016-03-02 2023-05-16 巴斯夫欧洲公司 制造l-草胺膦的方法

Also Published As

Publication number Publication date
TW202020148A (zh) 2020-06-01
CN112888780A (zh) 2021-06-01
KR20210038923A (ko) 2021-04-08
EP3830252A1 (en) 2021-06-09
IL280400A (en) 2021-03-01
WO2020025577A1 (en) 2020-02-06
TWI851589B (zh) 2024-08-11
JP7496814B2 (ja) 2024-06-07
US20220220457A1 (en) 2022-07-14
BR112021001800A2 (pt) 2021-05-04
JP2021532756A (ja) 2021-12-02

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