MX2015013057A - Composiciones y metodos de acidos nucleicos dirigidos al acido nucleico. - Google Patents
Composiciones y metodos de acidos nucleicos dirigidos al acido nucleico.Info
- Publication number
- MX2015013057A MX2015013057A MX2015013057A MX2015013057A MX2015013057A MX 2015013057 A MX2015013057 A MX 2015013057A MX 2015013057 A MX2015013057 A MX 2015013057A MX 2015013057 A MX2015013057 A MX 2015013057A MX 2015013057 A MX2015013057 A MX 2015013057A
- Authority
- MX
- Mexico
- Prior art keywords
- nucleic acid
- compositions
- methods
- targeting
- nucleic acids
- Prior art date
Links
- 150000007523 nucleic acids Chemical class 0.000 title abstract 5
- 108020004707 nucleic acids Proteins 0.000 title abstract 4
- 102000039446 nucleic acids Human genes 0.000 title abstract 4
- 238000000034 method Methods 0.000 title abstract 2
- 239000000203 mixture Substances 0.000 title abstract 2
- 238000010362 genome editing Methods 0.000 abstract 2
- 101710163270 Nuclease Proteins 0.000 abstract 1
- 230000004075 alteration Effects 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/43—Enzymes; Proenzymes; Derivatives thereof
- A61K38/46—Hydrolases (3)
- A61K38/465—Hydrolases (3) acting on ester bonds (3.1), e.g. lipases, ribonucleases
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
- A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
- A61K47/54—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic compound
- A61K47/549—Sugars, nucleosides, nucleotides or nucleic acids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
- A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
- A61K47/62—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being a protein, peptide or polyamino acid
- A61K47/64—Drug-peptide, drug-protein or drug-polyamino acid conjugates, i.e. the modifying agent being a peptide, protein or polyamino acid which is covalently bonded or complexed to a therapeutically active agent
- A61K47/645—Polycationic or polyanionic oligopeptides, polypeptides or polyamino acids, e.g. polylysine, polyarginine, polyglutamic acid or peptide TAT
- A61K47/6455—Polycationic oligopeptides, polypeptides or polyamino acids, e.g. for complexing nucleic acids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P11/00—Drugs for disorders of the respiratory system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P13/00—Drugs for disorders of the urinary system
- A61P13/12—Drugs for disorders of the urinary system of the kidneys
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P19/00—Drugs for skeletal disorders
- A61P19/02—Drugs for skeletal disorders for joint disorders, e.g. arthritis, arthrosis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P27/00—Drugs for disorders of the senses
- A61P27/02—Ophthalmic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
- A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0006—Modification of the membrane of cells, e.g. cell decoration
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/50—Physical structure
- C12N2310/53—Physical structure partially self-complementary or closed
- C12N2310/531—Stem-loop; Hairpin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/50—Physical structure
- C12N2310/53—Physical structure partially self-complementary or closed
- C12N2310/533—Physical structure partially self-complementary or closed having a mismatch or nick in at least one of the strands
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/203—Modifications characterised by incorporating a composite nucleic acid containing a polypeptide sequence other than PNA
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Plant Pathology (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Analytical Chemistry (AREA)
- Epidemiology (AREA)
- Mycology (AREA)
- Cell Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Heart & Thoracic Surgery (AREA)
- Cardiology (AREA)
- Rheumatology (AREA)
- Urology & Nephrology (AREA)
- Gastroenterology & Hepatology (AREA)
- Oncology (AREA)
Abstract
La presente invención se refiere a las composiciones y los métodos para usar ácidos nucleicos dirigidos al ácido nucleico y complejos de los mismos. El diseño del genoma se puede referirse a alterar el genoma mediante la supresión, inserción, mutación o sustitución de secuencias de ácidos nucleicos específicas,. La alteración puede ser específica del gen o de la localización. El diseño del genoma puede usar nucleasas para cortar un ácido nucleico, mediante lo cual se genera un sitio para la alteración. También se contempla el diseño de ácido nucleico no genómico.
Applications Claiming Priority (19)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201361781598P | 2013-03-14 | 2013-03-14 | |
US201361818386P | 2013-05-01 | 2013-05-01 | |
US201361818382P | 2013-05-01 | 2013-05-01 | |
US201361822002P | 2013-05-10 | 2013-05-10 | |
US201361832690P | 2013-06-07 | 2013-06-07 | |
US201361845714P | 2013-07-12 | 2013-07-12 | |
US201361858767P | 2013-07-26 | 2013-07-26 | |
US201361859661P | 2013-07-29 | 2013-07-29 | |
US201361865743P | 2013-08-14 | 2013-08-14 | |
US201361883804P | 2013-09-27 | 2013-09-27 | |
US201361899712P | 2013-11-04 | 2013-11-04 | |
US201361900311P | 2013-11-05 | 2013-11-05 | |
US201361902723P | 2013-11-11 | 2013-11-11 | |
US201361903232P | 2013-11-12 | 2013-11-12 | |
US201361906335P | 2013-11-19 | 2013-11-19 | |
US201361906211P | 2013-11-19 | 2013-11-19 | |
US201361907216P | 2013-11-21 | 2013-11-21 | |
US201361907777P | 2013-11-22 | 2013-11-22 | |
PCT/US2014/023828 WO2014150624A1 (en) | 2013-03-14 | 2014-03-12 | Compositions and methods of nucleic acid-targeting nucleic acids |
Publications (1)
Publication Number | Publication Date |
---|---|
MX2015013057A true MX2015013057A (es) | 2016-04-20 |
Family
ID=51580795
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX2015013057A MX2015013057A (es) | 2013-03-14 | 2014-03-12 | Composiciones y metodos de acidos nucleicos dirigidos al acido nucleico. |
Country Status (19)
Country | Link |
---|---|
US (17) | US9260752B1 (es) |
EP (2) | EP2971167B1 (es) |
JP (3) | JP2016519652A (es) |
KR (2) | KR20170108172A (es) |
CN (2) | CN105980575A (es) |
AU (3) | AU2014235794A1 (es) |
BR (1) | BR112015022061B8 (es) |
CA (1) | CA2905432C (es) |
DK (2) | DK3620534T3 (es) |
ES (2) | ES2901396T3 (es) |
GB (1) | GB2527450A (es) |
HK (1) | HK1213023A1 (es) |
IL (1) | IL241563B (es) |
MX (1) | MX2015013057A (es) |
NZ (1) | NZ712727A (es) |
RU (2) | RU2018122288A (es) |
SG (1) | SG11201507378UA (es) |
WO (1) | WO2014150624A1 (es) |
ZA (1) | ZA201507006B (es) |
Families Citing this family (307)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP2401367B1 (en) | 2009-02-26 | 2016-11-30 | Transposagen Biopharmaceuticals, Inc. | Hyperactive piggybac transposases |
EP3613852A3 (en) | 2011-07-22 | 2020-04-22 | President and Fellows of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
US11021737B2 (en) | 2011-12-22 | 2021-06-01 | President And Fellows Of Harvard College | Compositions and methods for analyte detection |
WO2014163886A1 (en) | 2013-03-12 | 2014-10-09 | President And Fellows Of Harvard College | Method of generating a three-dimensional nucleic acid containing matrix |
PL2847335T3 (pl) | 2012-04-25 | 2019-01-31 | Regeneron Pharmaceuticals, Inc. | Celowanie dużymi wektorami do celowania wspomagane nukleazą |
EP3346003B1 (en) * | 2012-10-23 | 2021-06-09 | Toolgen Incorporated | Composition for cleaving a target dna comprising a guide rna specific for the target dna and cas protein-encoding nucleic acid or cas protein, and use thereof |
PL2928496T3 (pl) | 2012-12-06 | 2020-04-30 | Sigma-Aldrich Co. Llc | Modyfikacja i regulacja genomu w oparciu o CRISPR |
EP2931892B1 (en) | 2012-12-12 | 2018-09-12 | The Broad Institute, Inc. | Methods, models, systems, and apparatus for identifying target sequences for cas enzymes or crispr-cas systems for target sequences and conveying results thereof |
DK2931897T3 (en) | 2012-12-12 | 2018-02-05 | Broad Inst Inc | CONSTRUCTION, MODIFICATION AND OPTIMIZATION OF SYSTEMS, PROCEDURES AND COMPOSITIONS FOR SEQUENCE MANIPULATION AND THERAPEUTICAL APPLICATIONS |
DK2931891T3 (da) | 2012-12-17 | 2019-08-19 | Harvard College | Rna-styret modificering af menneskelige genomer |
EP2946015B1 (en) | 2013-01-16 | 2021-05-26 | Emory University | Cas9-nucleic acid complexes and uses related thereto |
RU2018122288A (ru) | 2013-03-14 | 2019-03-06 | Карибо Биосайенсиз, Инк. | Композиции и способы с участием нуклеиновых кислот, нацеленных на нуклеиновые кислоты |
US10760064B2 (en) | 2013-03-15 | 2020-09-01 | The General Hospital Corporation | RNA-guided targeting of genetic and epigenomic regulatory proteins to specific genomic loci |
EP3741868B1 (en) | 2013-03-15 | 2024-05-22 | The General Hospital Corporation | Rna-guided targeting of genetic and epigenomic regulatory proteins to specific genomic loci |
JP2016522679A (ja) | 2013-04-04 | 2016-08-04 | プレジデント アンド フェローズ オブ ハーバード カレッジ | CRISPR/Cas系を用いたゲノム編集の治療的使用 |
US9902973B2 (en) | 2013-04-11 | 2018-02-27 | Caribou Biosciences, Inc. | Methods of modifying a target nucleic acid with an argonaute |
CN105308184B (zh) | 2013-04-16 | 2020-06-02 | 瑞泽恩制药公司 | 大鼠基因组的靶向修饰 |
US11414695B2 (en) | 2013-05-29 | 2022-08-16 | Agilent Technologies, Inc. | Nucleic acid enrichment using Cas9 |
US11685935B2 (en) * | 2013-05-29 | 2023-06-27 | Cellectis | Compact scaffold of Cas9 in the type II CRISPR system |
AU2014273082B2 (en) * | 2013-05-29 | 2018-11-08 | Cellectis | A method for producing precise DNA cleavage using Cas9 nickase activity |
US20140356956A1 (en) * | 2013-06-04 | 2014-12-04 | President And Fellows Of Harvard College | RNA-Guided Transcriptional Regulation |
AU2014274939B2 (en) | 2013-06-04 | 2020-03-19 | President And Fellows Of Harvard College | RNA-guideded transcriptional regulation |
SG11201510284XA (en) | 2013-06-17 | 2016-01-28 | Broad Inst Inc | Delivery and use of the crispr-cas systems, vectors and compositions for hepatic targeting and therapy |
WO2014204725A1 (en) | 2013-06-17 | 2014-12-24 | The Broad Institute Inc. | Optimized crispr-cas double nickase systems, methods and compositions for sequence manipulation |
EP3725885A1 (en) | 2013-06-17 | 2020-10-21 | The Broad Institute, Inc. | Functional genomics using crispr-cas systems, compositions methods, screens and applications thereof |
KR20160044457A (ko) | 2013-06-17 | 2016-04-25 | 더 브로드 인스티튜트, 인코퍼레이티드 | 서열 조작을 위한 탠덤 안내 시스템, 방법 및 조성물의 전달, 조작 및 최적화 |
CN105793425B (zh) | 2013-06-17 | 2021-10-26 | 布罗德研究所有限公司 | 使用病毒组分靶向障碍和疾病的crispr-cas系统和组合物的递送、用途和治疗应用 |
US10011850B2 (en) | 2013-06-21 | 2018-07-03 | The General Hospital Corporation | Using RNA-guided FokI Nucleases (RFNs) to increase specificity for RNA-Guided Genome Editing |
EP3019595A4 (en) | 2013-07-09 | 2016-11-30 | THERAPEUTIC USES OF A GENERIC CHANGE WITH CRISPR / CAS SYSTEMS | |
US11306328B2 (en) | 2013-07-26 | 2022-04-19 | President And Fellows Of Harvard College | Genome engineering |
US9163284B2 (en) | 2013-08-09 | 2015-10-20 | President And Fellows Of Harvard College | Methods for identifying a target site of a Cas9 nuclease |
US20150082478A1 (en) | 2013-08-22 | 2015-03-19 | E I Du Pont De Nemours And Company | Plant genome modification using guide rna/cas endonuclease systems and methods of use |
US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
US9526784B2 (en) | 2013-09-06 | 2016-12-27 | President And Fellows Of Harvard College | Delivery system for functional nucleases |
US9340799B2 (en) | 2013-09-06 | 2016-05-17 | President And Fellows Of Harvard College | MRNA-sensing switchable gRNAs |
US9322037B2 (en) | 2013-09-06 | 2016-04-26 | President And Fellows Of Harvard College | Cas9-FokI fusion proteins and uses thereof |
EP2877571B1 (en) | 2013-09-18 | 2018-05-30 | Kymab Limited | Methods, cells and organisms |
US10584358B2 (en) | 2013-10-30 | 2020-03-10 | North Carolina State University | Compositions and methods related to a type-II CRISPR-Cas system in Lactobacillus buchneri |
KR102523466B1 (ko) | 2013-11-07 | 2023-04-20 | 에디타스 메디신, 인코포레이티드 | 지배적인 gRNA를 이용하는 CRISPR-관련 방법 및 조성물 |
WO2015071474A2 (en) * | 2013-11-18 | 2015-05-21 | Crispr Therapeutics Ag | Crispr-cas system materials and methods |
US9074199B1 (en) | 2013-11-19 | 2015-07-07 | President And Fellows Of Harvard College | Mutant Cas9 proteins |
RU2685914C1 (ru) | 2013-12-11 | 2019-04-23 | Регенерон Фармасьютикалс, Инк. | Способы и композиции для направленной модификации генома |
KR20160097338A (ko) | 2013-12-12 | 2016-08-17 | 더 브로드 인스티튜트, 인코퍼레이티드 | 뉴클레오티드 반복 장애에서의 crispr-cas 시스템의 조성물 및 방법 및 용도 |
KR20160089527A (ko) | 2013-12-12 | 2016-07-27 | 더 브로드 인스티튜트, 인코퍼레이티드 | 게놈 편집을 위한 crispr-cas 시스템 및 조성물의 전달, 용도 및 치료적 응용 |
JP6793547B2 (ja) | 2013-12-12 | 2020-12-02 | ザ・ブロード・インスティテュート・インコーポレイテッド | 最適化機能CRISPR−Cas系による配列操作のための系、方法および組成物 |
WO2015089364A1 (en) | 2013-12-12 | 2015-06-18 | The Broad Institute Inc. | Crystal structure of a crispr-cas system, and uses thereof |
US20150166982A1 (en) | 2013-12-12 | 2015-06-18 | President And Fellows Of Harvard College | Methods for correcting pi3k point mutations |
US10787654B2 (en) | 2014-01-24 | 2020-09-29 | North Carolina State University | Methods and compositions for sequence guiding Cas9 targeting |
ES2802984T3 (es) | 2014-02-11 | 2021-01-22 | Univ Colorado Regents | Ingeniería del genoma multiplex mediante CRISPR |
EP3110945B1 (en) | 2014-02-27 | 2021-09-08 | Monsanto Technology LLC | Compositions and methods for site directed genomic modification |
US11028388B2 (en) | 2014-03-05 | 2021-06-08 | Editas Medicine, Inc. | CRISPR/Cas-related methods and compositions for treating Usher syndrome and retinitis pigmentosa |
US11141493B2 (en) | 2014-03-10 | 2021-10-12 | Editas Medicine, Inc. | Compositions and methods for treating CEP290-associated disease |
CA2948728A1 (en) | 2014-03-10 | 2015-09-17 | Editas Medicine, Inc. | Crispr/cas-related methods and compositions for treating leber's congenital amaurosis 10 (lca10) |
US11339437B2 (en) | 2014-03-10 | 2022-05-24 | Editas Medicine, Inc. | Compositions and methods for treating CEP290-associated disease |
WO2015148863A2 (en) | 2014-03-26 | 2015-10-01 | Editas Medicine, Inc. | Crispr/cas-related methods and compositions for treating sickle cell disease |
JP2017512481A (ja) | 2014-04-08 | 2017-05-25 | ノースカロライナ ステート ユニバーシティーNorth Carolina State University | Crispr関連遺伝子を用いた、rna依存性の転写抑制のための方法および組成物 |
KR101886381B1 (ko) * | 2014-05-28 | 2018-08-10 | 기초과학연구원 | 불활성화된 표적 특이적 뉴클레아제를 이용한 표적 dna의 분리 방법 |
CA2949713A1 (en) | 2014-05-30 | 2015-12-03 | The Board Of Trustees Of The Leland Stanford Junior University | Compositions and methods of delivering treatments for latent viral infections |
WO2015188065A1 (en) | 2014-06-05 | 2015-12-10 | Sangamo Biosciences, Inc. | Methods and compositions for nuclease design |
EP3155116A4 (en) * | 2014-06-10 | 2017-12-27 | Massachusetts Institute Of Technology | Method for gene editing |
CN106604994B (zh) | 2014-06-23 | 2021-12-14 | 通用医疗公司 | 通过测序评估的DSBs的全基因组无偏鉴定(GUIDE-Seq) |
CA2956224A1 (en) | 2014-07-30 | 2016-02-11 | President And Fellows Of Harvard College | Cas9 proteins including ligand-dependent inteins |
US10435685B2 (en) | 2014-08-19 | 2019-10-08 | Pacific Biosciences Of California, Inc. | Compositions and methods for enrichment of nucleic acids |
WO2016028843A2 (en) | 2014-08-19 | 2016-02-25 | President And Fellows Of Harvard College | Rna-guided systems for probing and mapping of nucleic acids |
EP3183367B1 (en) | 2014-08-19 | 2019-06-26 | Pacific Biosciences Of California, Inc. | Compositions and methods for enrichment of nucleic acids |
ES2730378T3 (es) | 2014-08-27 | 2019-11-11 | Caribou Biosciences Inc | Procedimientos para incrementar la eficiencia de la modificación mediada por Cas9 |
EP3186375A4 (en) | 2014-08-28 | 2019-03-13 | North Carolina State University | NEW CAS9 PROTEINS AND GUIDING ELEMENTS FOR DNA TARGETING AND THE GENOME EDITION |
MX2017002930A (es) | 2014-09-12 | 2017-06-06 | Du Pont | Generacion de sitios de integracion especifica de sitio para loci de rasgos complejos en maiz y soja, y metodos de uso. |
US20170306306A1 (en) * | 2014-10-24 | 2017-10-26 | Life Technologies Corporation | Compositions and Methods for Enhancing Homologous Recombination |
CN107429246B (zh) * | 2014-10-31 | 2021-06-01 | 麻省理工学院 | 用于crispr的大规模并行组合遗传学 |
AU2015342749B2 (en) | 2014-11-07 | 2022-01-27 | Editas Medicine, Inc. | Methods for improving CRISPR/Cas-mediated genome-editing |
KR102683423B1 (ko) | 2014-11-21 | 2024-07-10 | 리제너론 파마슈티칼스 인코포레이티드 | 쌍 형성된 가이드 rna를 사용하는 표적화된 유전자 변형을 위한 방법 및 조성물 |
KR102629128B1 (ko) | 2014-12-03 | 2024-01-25 | 애질런트 테크놀로지스, 인크. | 화학적 변형을 갖는 가이드 rna |
CN116059378A (zh) | 2014-12-10 | 2023-05-05 | 明尼苏达大学董事会 | 用于治疗疾病的遗传修饰的细胞、组织和器官 |
WO2016094872A1 (en) * | 2014-12-12 | 2016-06-16 | The Broad Institute Inc. | Dead guides for crispr transcription factors |
EP3230451B1 (en) | 2014-12-12 | 2021-04-07 | The Broad Institute, Inc. | Protected guide rnas (pgrnas) |
CA2970683A1 (en) * | 2014-12-18 | 2016-06-23 | Integrated Dna Technologies, Inc. | Crispr-based compositions and methods of use |
JP6947638B2 (ja) | 2014-12-20 | 2021-10-13 | アーク バイオ, エルエルシー | Crispr/cas系タンパク質を使用する核酸の標的化枯渇、富化および分割のための組成物および方法 |
EP3245232B1 (en) | 2015-01-12 | 2021-04-21 | The Regents of The University of California | Heterodimeric cas9 and methods of use thereof |
EP3250689B1 (en) | 2015-01-28 | 2020-11-04 | The Regents of The University of California | Methods and compositions for labeling a single-stranded target nucleic acid |
CA2975166C (en) | 2015-01-28 | 2020-01-14 | Pioneer Hi-Bred International, Inc. | Crispr hybrid dna/rna polynucleotides and methods of use |
KR20160103953A (ko) * | 2015-02-25 | 2016-09-02 | 연세대학교 산학협력단 | Crispr 시스템을 이용한 다중 위치 염기서열의 동시 포획 방법 |
AU2016226077B2 (en) | 2015-03-03 | 2021-12-23 | The General Hospital Corporation | Engineered CRISPR-Cas9 nucleases with altered PAM specificity |
CN113846144B (zh) | 2015-03-17 | 2023-09-26 | 生物辐射实验室股份有限公司 | 检测基因组编辑 |
CN107567499A (zh) | 2015-03-27 | 2018-01-09 | 纳幕尔杜邦公司 | 大豆u6核小rna基因启动子及其在植物小rna基因的组成型表达中的用途 |
US20180080051A1 (en) | 2015-03-31 | 2018-03-22 | Exeligen Scientific, Inc. | Cas 9 retroviral integrase and cas 9 recombinase systems for targeted incorporation of a dna sequence into a genome of a cell or organism |
CA2981715A1 (en) | 2015-04-06 | 2016-10-13 | The Board Of Trustees Of The Leland Stanford Junior University | Chemically modified guide rnas for crispr/cas-mediated gene regulation |
EP3286571B1 (en) | 2015-04-24 | 2021-08-18 | Editas Medicine, Inc. | Evaluation of cas9 molecule/guide rna molecule complexes |
AU2016261600B2 (en) | 2015-05-08 | 2021-09-23 | President And Fellows Of Harvard College | Universal donor stem cells and related methods |
WO2016182959A1 (en) | 2015-05-11 | 2016-11-17 | Editas Medicine, Inc. | Optimized crispr/cas9 systems and methods for gene editing in stem cells |
US10117911B2 (en) | 2015-05-29 | 2018-11-06 | Agenovir Corporation | Compositions and methods to treat herpes simplex virus infections |
EA201792663A1 (ru) | 2015-05-29 | 2018-04-30 | Норт Каролина Стейт Юниверсити | Способы скрининга бактерий, архей, водорослей и дрожжей с использованием нуклеиновых кислот crispr |
EP3303634B1 (en) | 2015-06-03 | 2023-08-30 | The Regents of The University of California | Cas9 variants and methods of use thereof |
EP3303585A4 (en) | 2015-06-03 | 2018-10-31 | Board of Regents of the University of Nebraska | Dna editing using single-stranded dna |
CA2986262A1 (en) | 2015-06-09 | 2016-12-15 | Editas Medicine, Inc. | Crispr/cas-related methods and compositions for improving transplantation |
US20160362667A1 (en) | 2015-06-10 | 2016-12-15 | Caribou Biosciences, Inc. | CRISPR-Cas Compositions and Methods |
EP3307762B1 (en) * | 2015-06-12 | 2021-12-15 | The Regents of The University of California | Reporter cas9 variants and methods of use thereof |
DK3307872T3 (da) | 2015-06-15 | 2023-10-23 | Univ North Carolina State | Fremgangsmåder og sammensætninger til effektiv indgivelse af nukleinsyrer og rna-baserede antimikrober |
US9790490B2 (en) | 2015-06-18 | 2017-10-17 | The Broad Institute Inc. | CRISPR enzymes and systems |
TW202400626A (zh) | 2015-06-18 | 2024-01-01 | 美商博得學院股份有限公司 | 降低脫靶效應的crispr酶突變 |
WO2016205759A1 (en) | 2015-06-18 | 2016-12-22 | The Broad Institute Inc. | Engineering and optimization of systems, methods, enzymes and guide scaffolds of cas9 orthologs and variants for sequence manipulation |
US20170000743A1 (en) * | 2015-07-02 | 2017-01-05 | Vindico NanoBio Technology Inc. | Compositions and Methods for Delivery of Gene Editing Tools Using Polymeric Vesicles |
US10456452B2 (en) | 2015-07-02 | 2019-10-29 | Poseida Therapeutics, Inc. | Compositions and methods for improved encapsulation of functional proteins in polymeric vesicles |
GB2595063B (en) | 2015-07-31 | 2022-03-09 | Univ Minnesota | Modified cells and methods of therapy |
CA2995036A1 (en) | 2015-08-06 | 2017-02-09 | Dana-Farber Cancer Institute, Inc. | Tunable endogenous protein degradation |
US9580727B1 (en) | 2015-08-07 | 2017-02-28 | Caribou Biosciences, Inc. | Compositions and methods of engineered CRISPR-Cas9 systems using split-nexus Cas9-associated polynucleotides |
US9926546B2 (en) | 2015-08-28 | 2018-03-27 | The General Hospital Corporation | Engineered CRISPR-Cas9 nucleases |
US9512446B1 (en) | 2015-08-28 | 2016-12-06 | The General Hospital Corporation | Engineered CRISPR-Cas9 nucleases |
JP6799586B2 (ja) | 2015-08-28 | 2020-12-16 | ザ ジェネラル ホスピタル コーポレイション | 遺伝子操作CRISPR−Cas9ヌクレアーゼ |
WO2017040709A1 (en) | 2015-08-31 | 2017-03-09 | Caribou Biosciences, Inc. | Directed nucleic acid repair |
WO2017044776A1 (en) * | 2015-09-10 | 2017-03-16 | Texas Tech University System | Single-guide rna (sgrna) with improved knockout efficiency |
CN105177110A (zh) * | 2015-09-11 | 2015-12-23 | 中国科学院微生物研究所 | 核酸的检测方法 |
WO2017044843A1 (en) | 2015-09-11 | 2017-03-16 | The General Hospital Corporation | Full interrogation of nuclease dsbs and sequencing (find-seq) |
US11667911B2 (en) | 2015-09-24 | 2023-06-06 | Editas Medicine, Inc. | Use of exonucleases to improve CRISPR/CAS-mediated genome editing |
US20180258411A1 (en) * | 2015-09-25 | 2018-09-13 | Tarveda Therapeutics, Inc. | Compositions and methods for genome editing |
EP3356533A1 (en) | 2015-09-28 | 2018-08-08 | North Carolina State University | Methods and compositions for sequence specific antimicrobials |
WO2017059313A1 (en) | 2015-09-30 | 2017-04-06 | The General Hospital Corporation | Comprehensive in vitro reporting of cleavage events by sequencing (circle-seq) |
CN116555254A (zh) | 2015-10-09 | 2023-08-08 | 孟山都技术公司 | 新颖的rna导向性核酸酶及其用途 |
DK3350327T3 (en) | 2015-10-23 | 2019-01-21 | Caribou Biosciences Inc | CONSTRUCTED CRISPR CLASS-2-NUCLEIC ACID TARGETING-NUCLEIC ACID |
WO2017070632A2 (en) | 2015-10-23 | 2017-04-27 | President And Fellows Of Harvard College | Nucleobase editors and uses thereof |
JP6882282B2 (ja) | 2015-11-03 | 2021-06-02 | プレジデント アンド フェローズ オブ ハーバード カレッジ | 三次元核酸含有マトリックスの立体撮像のための方法と装置 |
JP6929865B2 (ja) | 2015-11-13 | 2021-09-01 | タラ ムーア | 角膜ジストロフィーの治療方法 |
WO2017096328A1 (en) * | 2015-12-04 | 2017-06-08 | Caribou Biosciences, Inc. | Engineered nucleic-acid targeting nucleic acids |
WO2017112620A1 (en) | 2015-12-22 | 2017-06-29 | North Carolina State University | Methods and compositions for delivery of crispr based antimicrobials |
EP3402494B1 (en) | 2016-01-11 | 2021-04-07 | The Board of Trustees of the Leland Stanford Junior University | Chimeric proteins and methods of immunotherapy |
WO2017123559A2 (en) | 2016-01-11 | 2017-07-20 | The Board Of Trustees Of The Leland Stanford Junior University | Chimeric proteins and methods of regulating gene expression |
CA3011819A1 (en) | 2016-01-20 | 2017-07-27 | Vitrisa Therapeutics, Inc. | Compositions and methods for inhibiting factor d |
US20170218398A1 (en) * | 2016-01-30 | 2017-08-03 | Markus Alexander Brown | Method to selectively target cancerous cells for genetic manipulation |
AU2017213405B2 (en) * | 2016-01-30 | 2021-02-04 | Bonac Corporation | Artificial single guide RNA and use thereof |
WO2017139309A1 (en) | 2016-02-12 | 2017-08-17 | Ceres, Inc. | Methods and materials for high throughput testing of mutagenized allele combinations |
WO2017143071A1 (en) | 2016-02-18 | 2017-08-24 | The Regents Of The University Of California | Methods and compositions for gene editing in stem cells |
US10973930B2 (en) | 2016-02-18 | 2021-04-13 | The Penn State Research Foundation | Generating GABAergic neurons in brains |
ES2847252T3 (es) * | 2016-02-22 | 2021-08-02 | Caribou Biosciences Inc | Procedimientos de modulación de resultados de reparación de ADN |
WO2017149139A1 (en) * | 2016-03-03 | 2017-09-08 | Curevac Ag | Rna analysis by total hydrolysis |
EP3429567B1 (en) | 2016-03-16 | 2024-01-10 | The J. David Gladstone Institutes | Methods and compositions for treating obesity and/or diabetes and for identifying candidate treatment agents |
EP3433363A1 (en) | 2016-03-25 | 2019-01-30 | Editas Medicine, Inc. | Genome editing systems comprising repair-modulating enzyme molecules and methods of their use |
EP3433364A1 (en) | 2016-03-25 | 2019-01-30 | Editas Medicine, Inc. | Systems and methods for treating alpha 1-antitrypsin (a1at) deficiency |
US12031127B2 (en) * | 2016-04-08 | 2024-07-09 | Cold Spring Harbor Laboratory | Multiplexed analysis of neuron projections by sequencing |
MA45478A (fr) * | 2016-04-11 | 2019-02-20 | Arbutus Biopharma Corp | Compositions de conjugués d'acides nucléiques ciblés |
US11236313B2 (en) | 2016-04-13 | 2022-02-01 | Editas Medicine, Inc. | Cas9 fusion molecules, gene editing systems, and methods of use thereof |
WO2017189525A1 (en) | 2016-04-25 | 2017-11-02 | President And Fellows Of Harvard College | Hybridization chain reaction methods for in situ molecular detection |
EP3449004B1 (en) | 2016-04-29 | 2021-02-24 | Poseida Therapeutics, Inc. | Poly(histidine)-based micelles for complexation and delivery of proteins and nucleic acids |
US11608499B2 (en) * | 2016-04-29 | 2023-03-21 | Basf Plant Science Company Gmbh | Methods for modification of target nucleic acids |
CN116397007A (zh) * | 2016-05-11 | 2023-07-07 | 伊鲁米那股份有限公司 | 使用argonaute系统的多核苷酸富集和扩增 |
KR20190012229A (ko) | 2016-06-01 | 2019-02-08 | 케이더블유에스 사아트 에스이 | 유전자 조작을 위한 하이브리드 핵산 서열 |
KR20220147710A (ko) | 2016-06-02 | 2022-11-03 | 시그마-알드리치 컴퍼니., 엘엘씨 | 프로그램가능한 dna 결합 단백질을 사용한, 표적화된 게놈 변형의 개선 |
US10767175B2 (en) | 2016-06-08 | 2020-09-08 | Agilent Technologies, Inc. | High specificity genome editing using chemically modified guide RNAs |
US10337051B2 (en) | 2016-06-16 | 2019-07-02 | The Regents Of The University Of California | Methods and compositions for detecting a target RNA |
US20190323038A1 (en) * | 2016-06-17 | 2019-10-24 | Montana State Univesity | Bidirectional targeting for genome editing |
CA3029254A1 (en) | 2016-06-24 | 2017-12-28 | The Regents Of The University Of Colorado, A Body Corporate | Methods for generating barcoded combinatorial libraries |
EP3494220A1 (en) | 2016-08-02 | 2019-06-12 | Editas Medicine, Inc. | Compositions and methods for treating cep290 associated disease |
IL264565B2 (en) | 2016-08-03 | 2024-07-01 | Harvard College | Adenosine nuclear base editors and their uses |
AU2017308889B2 (en) | 2016-08-09 | 2023-11-09 | President And Fellows Of Harvard College | Programmable Cas9-recombinase fusion proteins and uses thereof |
US20190264193A1 (en) | 2016-08-12 | 2019-08-29 | Caribou Biosciences, Inc. | Protein engineering methods |
US20190309288A1 (en) * | 2016-08-18 | 2019-10-10 | The Board Of Trustees Of The Leland Stanford Junior University | Targeted mutagenesis |
WO2019165322A1 (en) * | 2018-02-22 | 2019-08-29 | Moore Tara | Crispr/cas9 systems, and methods of use thereof |
US11542509B2 (en) | 2016-08-24 | 2023-01-03 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
WO2018048194A1 (ko) * | 2016-09-07 | 2018-03-15 | 울산대학교 산학협력단 | dCas9 단백질 및 표적 핵산 서열에 결합하는 gRNA를 이용한 핵산 검출의 민감도 및 특이도 향상용 조성물 및 방법 |
WO2018053053A1 (en) * | 2016-09-13 | 2018-03-22 | The Broad Institute, Inc. | Proximity-dependent biotinylation and uses thereof |
WO2018053037A1 (en) * | 2016-09-13 | 2018-03-22 | The Jackson Laboratory | Targeted enhanced dna demethylation |
EP3532089A4 (en) | 2016-09-30 | 2020-05-13 | The Regents of The University of California | RNA-GUIDED NUCLEIC ACID MODIFYING ENZYMES AND METHOD FOR USE THEREOF |
MX2019003674A (es) | 2016-09-30 | 2021-01-08 | Univ California | Enzimas modificadoras de ácido nucleico guiadas por arn y métodos de uso de estas. |
US10669539B2 (en) | 2016-10-06 | 2020-06-02 | Pioneer Biolabs, Llc | Methods and compositions for generating CRISPR guide RNA libraries |
EP3526320A1 (en) | 2016-10-14 | 2019-08-21 | President and Fellows of Harvard College | Aav delivery of nucleobase editors |
GB2605540B (en) | 2016-10-18 | 2022-12-21 | Univ Minnesota | Tumor infiltrating lymphocytes and methods of therapy |
US10829729B2 (en) * | 2016-11-03 | 2020-11-10 | President And Fellows Of Harvard College | Cellular poration using laser radiation |
US9816093B1 (en) | 2016-12-06 | 2017-11-14 | Caribou Biosciences, Inc. | Engineered nucleic acid-targeting nucleic acids |
EP3336546B1 (en) * | 2016-12-13 | 2020-07-01 | Miltenyi Biotec B.V. & Co. KG | Reversible cell labelling with conjugates having two releasable binding sites |
EP3554546A4 (en) | 2016-12-14 | 2021-04-14 | Ligandal, Inc. | METHODS AND COMPOSITIONS FOR ADMINISTRATION OF NUCLEIC ACID AND PROTEIN PAYLOADS |
WO2018119359A1 (en) | 2016-12-23 | 2018-06-28 | President And Fellows Of Harvard College | Editing of ccr5 receptor gene to protect against hiv infection |
EP3342868B1 (en) | 2016-12-30 | 2019-12-25 | Systasy Bioscience GmbH | Constructs and screening methods |
WO2018129226A1 (en) * | 2017-01-05 | 2018-07-12 | Virginia Commonwealth University | System, method, computer-accessible medium and apparatus for dna mapping |
EP3565907B1 (en) | 2017-01-06 | 2022-05-04 | Editas Medicine, Inc. | Methods of assessing nuclease cleavage |
WO2018136827A1 (en) | 2017-01-20 | 2018-07-26 | Vitrisa Therapeutics, Inc. | Stem-loop compositions and methods for inhibiting factor d |
KR20190112771A (ko) * | 2017-01-30 | 2019-10-07 | 케이더블유에스 에스에이에이티 에스이 운트 코. 카게아아 | 게놈 조작을 위한 엔도뉴클레아제에 대한 복구 주형 연결 |
TW201839136A (zh) | 2017-02-06 | 2018-11-01 | 瑞士商諾華公司 | 治療血色素異常症之組合物及方法 |
EP3577220A2 (en) | 2017-02-06 | 2019-12-11 | Zymergen, Inc. | Engineered biosynthetic pathways for production of tyramine by fermentation |
JP7227912B2 (ja) | 2017-02-08 | 2023-02-24 | ダナ-ファーバー キャンサー インスティテュート,インコーポレイテッド | キメラ抗原受容体の調節 |
BR112019018240A2 (pt) * | 2017-03-02 | 2020-07-28 | MEIRAGTX, UK II Limited | regulação de expressão gene por clivagem de rnase p modulada por aptâmero |
WO2018165504A1 (en) | 2017-03-09 | 2018-09-13 | President And Fellows Of Harvard College | Suppression of pain by gene editing |
US11542496B2 (en) | 2017-03-10 | 2023-01-03 | President And Fellows Of Harvard College | Cytosine to guanine base editor |
EP3596217A1 (en) | 2017-03-14 | 2020-01-22 | Editas Medicine, Inc. | Systems and methods for the treatment of hemoglobinopathies |
IL269458B2 (en) | 2017-03-23 | 2024-02-01 | Harvard College | Nucleic base editors that include nucleic acid programmable DNA binding proteins |
BR112019015244A2 (pt) | 2017-03-24 | 2020-04-14 | Curevac Ag | ácidos nucleicos codificando proteínas associadas a crispr e usos dos mesmos |
WO2018183403A1 (en) | 2017-03-28 | 2018-10-04 | Caribou Biosciences, Inc. | Crispr-associated (cas) protein |
LT3589748T (lt) | 2017-04-13 | 2020-10-26 | Imba-Institut Für Molekulare Biotechnologie Gmbh | Nukleorūgšties modifikavimo ir identifikavimo būdas |
AU2018254547B2 (en) | 2017-04-20 | 2024-06-13 | Egenesis, Inc. | Methods for generating genetically modified animals |
WO2018195545A2 (en) | 2017-04-21 | 2018-10-25 | The General Hospital Corporation | Variants of cpf1 (cas12a) with altered pam specificity |
US20210123045A1 (en) * | 2017-04-25 | 2021-04-29 | The Johns Hopkins University | A yeast two-hybrid rna-protein interaction system based on catalytically inactivated crispr-dcas9 |
WO2018201086A1 (en) | 2017-04-28 | 2018-11-01 | Editas Medicine, Inc. | Methods and systems for analyzing guide rna molecules |
EP3622070A2 (en) | 2017-05-10 | 2020-03-18 | Editas Medicine, Inc. | Crispr/rna-guided nuclease systems and methods |
WO2018209320A1 (en) | 2017-05-12 | 2018-11-15 | President And Fellows Of Harvard College | Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation |
CN110662835B (zh) * | 2017-05-19 | 2023-04-28 | 清华大学 | 工程化改造用于由增强的指导RNA优化的基因编辑和转录调节的最小化SaCas9 CRISPR/Cas系统 |
JP2020521451A (ja) | 2017-05-25 | 2020-07-27 | ザ ジェネラル ホスピタル コーポレイション | 望ましくないオフターゲット塩基エディター脱アミノ化を制限するためのスプリットデアミナーゼの使用 |
US20200263186A1 (en) * | 2017-05-26 | 2020-08-20 | North Carolina State University | Altered guide rnas for modulating cas9 activity and methods of use |
MX2019014640A (es) | 2017-06-09 | 2020-10-05 | Editas Medicine Inc | Nucleasas de repeticiones palíndromicas agrupadas y regularmente interespaciadas de proteina asociada 9 (cas9) genomanipuladas. |
US10011849B1 (en) | 2017-06-23 | 2018-07-03 | Inscripta, Inc. | Nucleic acid-guided nucleases |
US9982279B1 (en) | 2017-06-23 | 2018-05-29 | Inscripta, Inc. | Nucleic acid-guided nucleases |
JP2020530307A (ja) | 2017-06-30 | 2020-10-22 | インティマ・バイオサイエンス,インコーポレーテッド | 遺伝子治療のためのアデノ随伴ウイルスベクター |
WO2019009682A2 (ko) * | 2017-07-07 | 2019-01-10 | 주식회사 툴젠 | 표적 특이적 crispr 변이체 |
US11866726B2 (en) | 2017-07-14 | 2024-01-09 | Editas Medicine, Inc. | Systems and methods for targeted integration and genome editing and detection thereof using integrated priming sites |
WO2019036140A1 (en) | 2017-07-17 | 2019-02-21 | Zymergen Inc. | METALLO-ORGANIC STRESS MATERIALS |
WO2019022986A1 (en) * | 2017-07-24 | 2019-01-31 | Vitrisa Therapeutics, Inc. | NUCLEIC ACID COMPOSITIONS AND METHODS OF INHIBITING D-FACTOR |
JP2020534795A (ja) | 2017-07-28 | 2020-12-03 | プレジデント アンド フェローズ オブ ハーバード カレッジ | ファージによって支援される連続的進化(pace)を用いて塩基編集因子を進化させるための方法および組成物 |
US20210087557A1 (en) * | 2017-08-04 | 2021-03-25 | Syngenta Participations Ag | Methods and compositions for targeted genomic insertion |
CN111278450B (zh) | 2017-08-23 | 2024-04-09 | 通用医疗公司 | 具有改变的PAM特异性的工程化的CRISPR-Cas9核酸酶 |
WO2019139645A2 (en) | 2017-08-30 | 2019-07-18 | President And Fellows Of Harvard College | High efficiency base editors comprising gam |
JP2020534812A (ja) * | 2017-09-08 | 2020-12-03 | ライフ テクノロジーズ コーポレイション | 改良された相同組換えおよびその組成物のための方法 |
US11649442B2 (en) | 2017-09-08 | 2023-05-16 | The Regents Of The University Of California | RNA-guided endonuclease fusion polypeptides and methods of use thereof |
US20190218532A1 (en) * | 2017-09-19 | 2019-07-18 | Massachusetts Institute Of Technology | Streptococcus Canis Cas9 as a Genome Engineering Platform with Novel PAM Specificity |
CA3078845A1 (en) * | 2017-10-09 | 2019-04-18 | Pioneer Hi-Bred International, Inc. | Type i-e crispr-cas systems for eukaryotic genome editing |
US11725228B2 (en) | 2017-10-11 | 2023-08-15 | The General Hospital Corporation | Methods for detecting site-specific and spurious genomic deamination induced by base editing technologies |
EP3697906A1 (en) | 2017-10-16 | 2020-08-26 | The Broad Institute, Inc. | Uses of adenosine base editors |
AU2018358051A1 (en) | 2017-11-01 | 2020-05-14 | The Regents Of The University Of California | CasZ compositions and methods of use |
WO2019089808A1 (en) | 2017-11-01 | 2019-05-09 | The Regents Of The University Of California | Class 2 crispr/cas compositions and methods of use |
WO2019090237A1 (en) * | 2017-11-03 | 2019-05-09 | Genus Plc | Selectively targeted dna edits in livestock |
EP3720453B1 (en) | 2017-12-05 | 2024-07-10 | Caribou Biosciences, Inc. | Modified lymphocytes |
EP3720944A1 (en) | 2017-12-07 | 2020-10-14 | Zymergen Inc. | Engineered biosynthetic pathways for production of (6e)-8-hydroxygeraniol by fermentation |
US11661599B1 (en) | 2017-12-14 | 2023-05-30 | National Technology & Engineering Solutions Of Sandia, Llc | CRISPR-Cas based system for targeting single-stranded sequences |
WO2019126037A1 (en) * | 2017-12-19 | 2019-06-27 | City Of Hope | Modified tracrrnas grnas, and uses thereof |
RU2652899C1 (ru) * | 2017-12-28 | 2018-05-03 | Федеральное бюджетное учреждение науки "Центральный научно-исследовательский институт эпидемиологии" Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человека (ФБУН ЦНИИ Эпидемиологии Роспотребнадзора) | РНК-проводники для подавления репликации вируса гепатита B и для элиминации вируса гепатита B из клетки-хозяина |
US20210093679A1 (en) | 2018-02-05 | 2021-04-01 | Novome Biotechnologies, Inc. | Engineered gut microbes and uses thereof |
KR20200124702A (ko) | 2018-02-23 | 2020-11-03 | 파이어니어 하이 부렛드 인터내쇼날 인코포레이팃드 | 신규한 cas9 오르소로그 |
WO2019173248A1 (en) | 2018-03-07 | 2019-09-12 | Caribou Biosciences, Inc. | Engineered nucleic acid-targeting nucleic acids |
US20210010064A1 (en) * | 2018-03-14 | 2021-01-14 | The Trustees Of The University Of Pennsylvania | Enrichment of nucleic acids |
KR20200132924A (ko) | 2018-03-14 | 2020-11-25 | 에디타스 메디신, 인코포레이티드 | 혈색소병증 치료를 위한 시스템 및 방법 |
JP7558563B2 (ja) | 2018-03-15 | 2024-10-01 | ケーエスキュー セラピューティクス, インコーポレイテッド | 免疫療法の改善のための遺伝子調節組成物及び遺伝子調節方法 |
EP3781677A4 (en) * | 2018-04-16 | 2022-01-19 | University of Massachusetts | COMPOSITIONS AND METHODS FOR IMPROVED GENE EDITTING |
EP3781585A4 (en) | 2018-04-17 | 2022-01-26 | The General Hospital Corporation | SENSITIVE IN VITRO TESTS FOR SUBSTRATE PREFERENCES AND SITE FOR NUCLEIC ACID BINDERS, MODIFIERS AND CLEAVAGE AGENTS |
US11160260B2 (en) | 2018-04-17 | 2021-11-02 | The Curators Of The University Of Missouri | Methods for protecting porcine fetuses from infection with porcine reproductive and respiratory syndrome virus (PRRSV) |
CN108588123A (zh) * | 2018-05-07 | 2018-09-28 | 南京医科大学 | CRISPR/Cas9载体组合在制备基因敲除猪的血液制品中的应用 |
CN112654710A (zh) | 2018-05-16 | 2021-04-13 | 辛瑟高公司 | 用于指导rna设计和使用的方法和系统 |
US10227576B1 (en) | 2018-06-13 | 2019-03-12 | Caribou Biosciences, Inc. | Engineered cascade components and cascade complexes |
EP3601574A4 (en) | 2018-06-13 | 2020-03-18 | Caribou Biosciences, Inc. | TECHNICAL CASCADE COMPONENTS AND CASCADE COMPLEXES |
US20210269782A1 (en) * | 2018-06-26 | 2021-09-02 | The Regents Of The University Of California | Rna-guided effector proteins and methods of use thereof |
US20210285009A1 (en) | 2018-07-13 | 2021-09-16 | The Regents Of The University Of California | Retrotransposon-based delivery vehicle and methods of use thereof |
WO2020028729A1 (en) | 2018-08-01 | 2020-02-06 | Mammoth Biosciences, Inc. | Programmable nuclease compositions and methods of use thereof |
WO2020047124A1 (en) | 2018-08-28 | 2020-03-05 | Flagship Pioneering, Inc. | Methods and compositions for modulating a genome |
US20210180045A1 (en) * | 2018-08-31 | 2021-06-17 | The Children's Hospital Of Philadelphia | Scalable tagging of endogenous genes by homology-independent intron targeting |
WO2020068643A1 (en) * | 2018-09-25 | 2020-04-02 | Excision Biotherapeutics, Inc. | CRISPRs WITH IMPROVED SPECIFICITY |
US10711267B2 (en) | 2018-10-01 | 2020-07-14 | North Carolina State University | Recombinant type I CRISPR-Cas system |
WO2020076976A1 (en) | 2018-10-10 | 2020-04-16 | Readcoor, Inc. | Three-dimensional spatial molecular indexing |
US20220290113A1 (en) * | 2018-10-15 | 2022-09-15 | University Of Massachusetts | Programmable dna base editing by nme2cas9-deaminase fusion proteins |
US11407995B1 (en) | 2018-10-26 | 2022-08-09 | Inari Agriculture Technology, Inc. | RNA-guided nucleases and DNA binding proteins |
US11434477B1 (en) | 2018-11-02 | 2022-09-06 | Inari Agriculture Technology, Inc. | RNA-guided nucleases and DNA binding proteins |
IL283853B2 (en) | 2018-12-14 | 2024-10-01 | Pioneer Hi Bred Int | Novel CRISPR-CAS systems for genome editing |
EP3898987A1 (en) | 2018-12-20 | 2021-10-27 | Basf Plant Science Company GmbH | Native delivery of biomolecules into plant cells using ionic complexes with cell-penetrating peptides |
RU2714380C1 (ru) * | 2018-12-28 | 2020-02-14 | Федеральное государственное бюджетное учреждение науки институт биоорганической химии им. академиков М.М. Шемякина и Ю.А. Овчинникова Российской академии наук (ИБХ РАН) | Способ получения цитотоксических т-лимфоцитов, экспрессирующих химерные рецепторы |
EP3931313A2 (en) | 2019-01-04 | 2022-01-05 | Mammoth Biosciences, Inc. | Programmable nuclease improvements and compositions and methods for nucleic acid amplification and detection |
US11946040B2 (en) | 2019-02-04 | 2024-04-02 | The General Hospital Corporation | Adenine DNA base editor variants with reduced off-target RNA editing |
US20220145330A1 (en) | 2019-02-10 | 2022-05-12 | The J. David Gladstone Institutes, a testamentary trust established under the Will of J. David Glads | Modified mitochondrion and methods of use thereof |
US20220290120A1 (en) | 2019-02-25 | 2022-09-15 | Novome Biotechnologies, Inc. | Plasmids for gene editing |
EP3935184B1 (en) * | 2019-03-04 | 2024-09-11 | King Abdullah University Of Science And Technology | Compositions and methods of targeted nucleic acid enrichment by loop adapter protection and exonuclease digestion |
EP3935156A4 (en) | 2019-03-07 | 2022-12-28 | The Regents of The University of California | CRISPR-CAS EFFECTOR POLYPEPTIDES AND METHODS OF USE |
JP2022527740A (ja) | 2019-03-19 | 2022-06-06 | ザ ブロード インスティテュート,インコーポレーテッド | 編集ヌクレオチド配列を編集するための方法および組成物 |
EP3979829A4 (en) * | 2019-06-06 | 2023-06-28 | Tata Consultancy Services Limited | System and method for combating pseudomonas aeruginosa and staphylococcus aureus infrctions |
WO2020245781A2 (en) * | 2019-06-06 | 2020-12-10 | Tata Consultancy Services Limited | System and method for combating infections due to antibiotic induced pathogens |
CN113939313B (zh) * | 2019-06-06 | 2024-11-01 | 电化株式会社 | 以肽核酸为基础的佐剂 |
WO2020245763A2 (en) * | 2019-06-06 | 2020-12-10 | Tata Consultancy Services Limited | System and method for combating mycobacterium tuberculosis infections |
US20220389484A1 (en) * | 2019-06-06 | 2022-12-08 | Tata Consultancy Services Limited | System and method for combating infections due to pathogens belonging to phylum proteobacteria |
EP3987024A4 (en) | 2019-06-20 | 2023-11-01 | University Of Massachusetts | COMPOSITIONS AND METHODS FOR IMPROVED GENE EDITING |
US20230000908A1 (en) * | 2019-09-11 | 2023-01-05 | Ohio State Innovation Foundation | Engineered cells and uses thereof |
JP2022169813A (ja) * | 2019-09-30 | 2022-11-10 | 国立研究開発法人産業技術総合研究所 | Cas9ヌクレアーゼのDNA切断活性を制御する方法 |
WO2021091974A1 (en) * | 2019-11-08 | 2021-05-14 | The Regents Of The University Of California | Compositions and methods for temporal control of cell modulation |
WO2021097118A1 (en) * | 2019-11-12 | 2021-05-20 | The Broad Institute, Inc. | Small type ii cas proteins and methods of use thereof |
US20230037413A1 (en) | 2019-11-20 | 2023-02-09 | Corbion Biotech, Inc. | Sucrose invertase variants |
BR112022006229A2 (pt) | 2019-11-29 | 2022-06-21 | Basf Se | Métodos para conferir ou aumentar a resistência a fungos, controlar um fungo e cultivar uma planta, construção de vetor recombinante, vetor de expressão recombinante, planta geneticamente modificada, planta de cultivo, métodos para a produção de uma planta e para a produção de um produto, uso de uma proteína, parte colhível de uma planta, produto derivado de uma planta e método de avaliação de uma planta |
EP4077651A1 (en) | 2019-12-16 | 2022-10-26 | BASF Agricultural Solutions Seed US LLC | Codon-optimized cas9 endonuclease encoding polynucleotide |
CN115485387A (zh) | 2019-12-19 | 2022-12-16 | 巴斯夫欧洲公司 | 增加精细化学品生产中的时空产率、碳转化效率和碳底物灵活性 |
BR112022011895A2 (pt) | 2019-12-20 | 2022-09-06 | Basf Se | Diminuição da toxidez de terpenos e aumento da produção potencial em microrganismos |
EP4093864A4 (en) * | 2020-01-24 | 2024-04-10 | The General Hospital Corporation | UNCONSTRAINED GENOME TARGETING WITH GENETICALLY MODIFIED CRISPR-CAS9 VARIANTS NEARLY PAM-FREE |
WO2021151756A1 (en) * | 2020-01-27 | 2021-08-05 | Eth Zurich | Base editor lacking hnh and use thereof |
US20230212593A1 (en) | 2020-03-04 | 2023-07-06 | Basf Se | Method for the production of constitutive bacterial promoters conferring low to medium expression |
EP4114956A1 (en) | 2020-03-04 | 2023-01-11 | Basf Se | Shuttle vector for expression in e. coli and bacilli |
CA3174483A1 (en) | 2020-03-04 | 2021-09-10 | Flagship Pioneering Innovations Vi, Llc | Improved methods and compositions for modulating a genome |
EP4121522A4 (en) | 2020-03-19 | 2024-06-19 | Intellia Therapeutics, Inc. | METHODS AND COMPOSITIONS FOR DIRECTED GENOMIC EDITING |
US20230134582A1 (en) * | 2020-04-09 | 2023-05-04 | Verve Therapeutics, Inc. | Chemically modified guide rnas for genome editing with cas12b |
IL297761A (en) | 2020-05-08 | 2022-12-01 | Broad Inst Inc | Methods and compositions for simultaneously editing two helices of a designated double-helix nucleotide sequence |
WO2022074056A2 (en) | 2020-10-07 | 2022-04-14 | Basf Se | Bacillus cell with reduced lipase and/or esterase side activities |
JP2023546597A (ja) | 2020-10-21 | 2023-11-06 | マサチューセッツ インスティテュート オブ テクノロジー | 部位特異的標的化エレメントによるプログラム可能な付加(paste)を使用した部位特異的遺伝子操作のためのシステム、方法及び組成物 |
CN117042600A (zh) | 2020-11-16 | 2023-11-10 | 猪改良英国有限公司 | 具有经编辑的anp32基因的抗甲型流感动物 |
TW202246497A (zh) * | 2021-01-20 | 2022-12-01 | 美商阿伯生物技術公司 | Cas12i2融合分子及其用途 |
CN117460518A (zh) * | 2021-04-30 | 2024-01-26 | 宾夕法尼亚大学理事会 | Cd-90靶向的脂质纳米颗粒 |
BR112023024985A2 (pt) | 2021-06-01 | 2024-02-20 | Arbor Biotechnologies Inc | Sistemas de edição de genes compreendendo uma crispr nuclease e usos dos mesmos |
EP4359546A1 (en) | 2021-06-24 | 2024-05-01 | Basf Se | Improved bacillus host cell with altered rema/remb protein |
WO2022269081A1 (en) | 2021-06-24 | 2022-12-29 | Basf Se | Bacillus licheniformis host cell for production of a compound of interest with increased purity |
JP2024533311A (ja) | 2021-09-08 | 2024-09-12 | フラッグシップ パイオニアリング イノベーションズ シックス,エルエルシー | ゲノムを調節するための方法及び組成物 |
KR20240055811A (ko) | 2021-09-10 | 2024-04-29 | 애질런트 테크놀로지스, 인크. | 프라임 편집을 위한 화학적 변형을 갖는 가이드 rna |
MX2024005030A (es) | 2021-10-26 | 2024-05-13 | Caribou Biosciences Inc | Ensayo de actividad de endonucleasa en tiempo real acoplado a exonucleasa. |
WO2023081756A1 (en) | 2021-11-03 | 2023-05-11 | The J. David Gladstone Institutes, A Testamentary Trust Established Under The Will Of J. David Gladstone | Precise genome editing using retrons |
WO2023102329A2 (en) * | 2021-11-30 | 2023-06-08 | Mammoth Biosciences, Inc. | Effector proteins and uses thereof |
AU2022407332B2 (en) | 2021-12-07 | 2024-10-03 | Caribou Biosciences, Inc. | A method of capturing crispr endonuclease cleavage products |
US20230279442A1 (en) | 2021-12-15 | 2023-09-07 | Versitech Limited | Engineered cas9-nucleases and method of use thereof |
CN118434872A (zh) | 2021-12-20 | 2024-08-02 | 巴斯夫欧洲公司 | 用于提高芽孢杆菌中胞内蛋白质产量的方法 |
WO2023141602A2 (en) | 2022-01-21 | 2023-07-27 | Renagade Therapeutics Management Inc. | Engineered retrons and methods of use |
WO2023173062A2 (en) * | 2022-03-11 | 2023-09-14 | Intima Bioscience, Inc. | Nucleic acid editing systems, methods, and uses thereof |
WO2023230529A1 (en) | 2022-05-26 | 2023-11-30 | Caribou Biosciences, Inc. | Cytokine-receptor fusions for immune cell stimulation |
WO2023230316A1 (en) * | 2022-05-27 | 2023-11-30 | The Broad Institute, Inc. | Ribozyme-assisted circular rnas and compositions and methods of use there of |
WO2023240042A1 (en) | 2022-06-06 | 2023-12-14 | Caribou Biosciences, Inc. | Treatment of autoimmune diseases with engineered immune cells |
GB202209518D0 (en) | 2022-06-29 | 2022-08-10 | Snipr Biome Aps | Treating & preventing E coli infections |
WO2024020346A2 (en) | 2022-07-18 | 2024-01-25 | Renagade Therapeutics Management Inc. | Gene editing components, systems, and methods of use |
WO2024044723A1 (en) | 2022-08-25 | 2024-02-29 | Renagade Therapeutics Management Inc. | Engineered retrons and methods of use |
WO2024073583A1 (en) | 2022-09-30 | 2024-04-04 | Caribou Biosciences, Inc. | Anti-ror1 chimeric antigen receptors (cars), car-nk cells and related methods |
WO2024107646A1 (en) | 2022-11-14 | 2024-05-23 | Caribou Biosciences, Inc. | Anti-cll-1 chimeric antigen receptors, engineered cells and related methods |
WO2024107665A1 (en) * | 2022-11-14 | 2024-05-23 | Mammoth Biosciences, Inc. | Effector proteins, compositions, systems and methods of use thereof |
WO2024173645A1 (en) | 2023-02-15 | 2024-08-22 | Arbor Biotechnologies, Inc. | Gene editing method for inhibiting aberrant splicing in stathmin 2 (stmn2) transcript |
US20240301373A1 (en) * | 2023-03-08 | 2024-09-12 | Massachusetts Institute Of Technology | Fanzors are rna-guided nucleases encoded in eukaryotic genomes |
Family Cites Families (150)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5034506A (en) | 1985-03-15 | 1991-07-23 | Anti-Gene Development Group | Uncharged morpholino-based polymers having achiral intersubunit linkages |
WO1988008450A1 (en) | 1987-05-01 | 1988-11-03 | Birdwell Finlayson | Gene therapy for metabolite disorders |
US5350689A (en) | 1987-05-20 | 1994-09-27 | Ciba-Geigy Corporation | Zea mays plants and transgenic Zea mays plants regenerated from protoplasts or protoplast-derived cells |
US5767367A (en) | 1990-06-23 | 1998-06-16 | Hoechst Aktiengesellschaft | Zea mays (L.) with capability of long term, highly efficient plant regeneration including fertile transgenic maize plants having a heterologous gene, and their preparation |
US5489677A (en) | 1990-07-27 | 1996-02-06 | Isis Pharmaceuticals, Inc. | Oligonucleoside linkages containing adjacent oxygen and nitrogen atoms |
US5602240A (en) | 1990-07-27 | 1997-02-11 | Ciba Geigy Ag. | Backbone modified oligonucleotide analogs |
FR2688514A1 (fr) | 1992-03-16 | 1993-09-17 | Centre Nat Rech Scient | Adenovirus recombinants defectifs exprimant des cytokines et medicaments antitumoraux les contenant. |
US5625048A (en) | 1994-11-10 | 1997-04-29 | The Regents Of The University Of California | Modified green fluorescent proteins |
US5968738A (en) | 1995-12-06 | 1999-10-19 | The Board Of Trustees Of The Leland Stanford Junior University | Two-reporter FACS analysis of mammalian cells using green fluorescent proteins |
US20020182673A1 (en) | 1998-05-15 | 2002-12-05 | Genentech, Inc. | IL-17 homologous polypedies and therapeutic uses thereof |
US6306610B1 (en) | 1998-09-18 | 2001-10-23 | Massachusetts Institute Of Technology | Biological applications of quantum dots |
EP1147209A2 (en) | 1999-02-03 | 2001-10-24 | The Children's Medical Center Corporation | Gene repair involving the induction of double-stranded dna cleavage at a chromosomal target site |
IL150381A0 (en) | 1999-12-28 | 2002-12-01 | Ribonomics Inc | METHODS FOR ISOLATING AND CHARACTERIZING ENDOGENOUS mRNA-PROTEIN (mRNP) COMPLEXES |
EP1152058A1 (en) * | 2000-05-03 | 2001-11-07 | Institut Curie | Methods and compositions for effecting homologous recombination |
WO2002026967A2 (en) | 2000-09-25 | 2002-04-04 | Thomas Jefferson University | Targeted gene correction by single-stranded oligodeoxynucleotides |
MX357775B (es) | 2000-10-27 | 2018-07-20 | J Craig Venter Inst Inc | Acidos nucleicos y proteinas de los grupos a y b de estreptococos. |
US20060253913A1 (en) | 2001-12-21 | 2006-11-09 | Yue-Jin Huang | Production of hSA-linked butyrylcholinesterases in transgenic mammals |
US8106255B2 (en) | 2002-01-23 | 2012-01-31 | Dana Carroll | Targeted chromosomal mutagenasis using zinc finger nucleases |
EP1334979A1 (en) * | 2002-02-08 | 2003-08-13 | Kweek-en Researchbedrijf Agrico B.V. | Gene conferring resistance to Phytophthera infestans (late-blight) in Solanaceae |
US20030232410A1 (en) | 2002-03-21 | 2003-12-18 | Monika Liljedahl | Methods and compositions for using zinc finger endonucleases to enhance homologous recombination |
AU2003224897A1 (en) | 2002-04-09 | 2003-10-27 | Kenneth L. Beattie | Oligonucleotide probes for genosensor chips |
US9447434B2 (en) | 2002-09-05 | 2016-09-20 | California Institute Of Technology | Use of chimeric nucleases to stimulate gene targeting |
US20120196370A1 (en) | 2010-12-03 | 2012-08-02 | Fyodor Urnov | Methods and compositions for targeted genomic deletion |
WO2005030999A1 (en) * | 2003-09-25 | 2005-04-07 | Dana-Farber Cancer Institute, Inc | Methods to detect lineage-specific cells |
US7282333B2 (en) | 2003-10-20 | 2007-10-16 | Promega Corporation | Methods and compositions for nucleic acid analysis |
US7972854B2 (en) | 2004-02-05 | 2011-07-05 | Sangamo Biosciences, Inc. | Methods and compositions for targeted cleavage and recombination |
US7919277B2 (en) | 2004-04-28 | 2011-04-05 | Danisco A/S | Detection and typing of bacterial strains |
US7892224B2 (en) | 2005-06-01 | 2011-02-22 | Brainlab Ag | Inverse catheter planning |
CN101273141B (zh) | 2005-07-26 | 2013-03-27 | 桑格摩生物科学股份有限公司 | 外源核酸序列的靶向整合和表达 |
AU2006282983B2 (en) | 2005-08-26 | 2012-08-02 | Dupont Nutrition Biosciences Aps | Use |
US9193974B2 (en) | 2006-05-10 | 2015-11-24 | Deinove | Process for chromosomal engineering using a novel dna repair system |
EP2018441B1 (en) | 2006-05-19 | 2011-10-26 | Danisco A/S | Tagged microorganisms and methods of tagging |
EP2765195A1 (en) | 2006-05-25 | 2014-08-13 | Sangamo BioSciences, Inc. | Methods and compositions for gene inactivation |
CN101505607B (zh) | 2006-06-16 | 2013-06-26 | 杜邦营养生物科学有限公司 | 细菌 |
EP2054514A4 (en) | 2006-08-04 | 2009-11-04 | Univ Georgia State Res Found | ENZYME SENSORS, METHODS OF PREPARATION AND USE OF SUCH SENSORS, AND METHODS OF DETECTING PROTEASE ACTIVITY |
DE102006051516A1 (de) * | 2006-10-31 | 2008-05-08 | Curevac Gmbh | (Basen-)modifizierte RNA zur Expressionssteigerung eines Proteins |
JP5932207B2 (ja) | 2007-03-02 | 2016-06-08 | デュポン ニュートリション バイオサイエンシス エーピーエス | 改善されたファージ耐性をもつ培養物 |
CA2682761C (en) | 2007-04-04 | 2015-10-13 | Network Biosystems, Inc. | Methods for rapid multiplexed amplification of target nucleic acids |
FR2925918A1 (fr) | 2007-12-28 | 2009-07-03 | Pasteur Institut | Typage et sous-typage moleculaire de salmonella par identification des sequences nucleotidiques variables des loci crispr |
US8546553B2 (en) | 2008-07-25 | 2013-10-01 | University Of Georgia Research Foundation, Inc. | Prokaryotic RNAi-like system and methods of use |
CN102159722B (zh) | 2008-08-22 | 2014-09-03 | 桑格摩生物科学股份有限公司 | 用于靶向单链切割和靶向整合的方法和组合物 |
WO2010030963A2 (en) | 2008-09-15 | 2010-03-18 | Children's Medical Center Corporation | Modulation of bcl11a for treatment of hemoglobinopathies |
US20100076057A1 (en) | 2008-09-23 | 2010-03-25 | Northwestern University | TARGET DNA INTERFERENCE WITH crRNA |
US9404098B2 (en) | 2008-11-06 | 2016-08-02 | University Of Georgia Research Foundation, Inc. | Method for cleaving a target RNA using a Cas6 polypeptide |
RU2570562C2 (ru) | 2008-11-07 | 2015-12-10 | ДюПон НЬЮТРИШН БАЙОСАЙЕНСИЗ АпС | Последовательности crispr бифидобактерий |
US8129149B1 (en) * | 2008-11-12 | 2012-03-06 | The United States Of America As Represented By The Secretary Of The Army | Rapid and sensitive method to measure endonuclease activity |
US8771766B2 (en) | 2008-12-12 | 2014-07-08 | Dupont Nutrition Biosciences Aps | Genetic cluster of strains of Streptococcus thermophilus having unique rheological properties for dairy fermentation |
WO2010075424A2 (en) | 2008-12-22 | 2010-07-01 | The Regents Of University Of California | Compositions and methods for downregulating prokaryotic genes |
EP2419511B1 (en) | 2009-04-09 | 2018-01-17 | Sangamo Therapeutics, Inc. | Targeted integration into stem cells |
EA023938B1 (ru) | 2009-04-30 | 2016-07-29 | Оспедале Сан Рафаэлле С.Р.Л. | Вектор гена |
WO2010143917A2 (en) | 2009-06-11 | 2010-12-16 | Toolgen Incorporation | Targeted genomic rearrangements using site-specific nucleases |
WO2011011767A1 (en) | 2009-07-24 | 2011-01-27 | Sigma-Aldrich Co. | Method for genome editing |
US20120192298A1 (en) | 2009-07-24 | 2012-07-26 | Sigma Aldrich Co. Llc | Method for genome editing |
EP2727600B1 (en) | 2009-07-28 | 2019-03-27 | Sangamo Therapeutics, Inc. | Zinc finger fusion proteins for repressing a huntington gene |
CN104830894B (zh) | 2009-08-11 | 2019-03-01 | 桑格摩生物治疗股份有限公司 | 靶向修饰的纯合生物体 |
DK2292731T3 (en) | 2009-08-13 | 2018-07-23 | Dupont Nutrition Biosci Aps | Process for producing complex cultures |
CA2773879A1 (en) | 2009-09-25 | 2011-03-31 | Basf Plant Science Company Gmbh | Plants having enhanced yield-related traits and a method for making the same |
GB2487341A (en) | 2009-11-02 | 2012-07-18 | Nugen Technologies Inc | Compositions and methods for targeted nucleic acid sequence selection and amplification |
WO2011064751A1 (en) | 2009-11-27 | 2011-06-03 | Basf Plant Science Company Gmbh | Chimeric endonucleases and uses thereof |
US20110294114A1 (en) | 2009-12-04 | 2011-12-01 | Cincinnati Children's Hospital Medical Center | Optimization of determinants for successful genetic correction of diseases, mediated by hematopoietic stem cells |
PL2816112T3 (pl) | 2009-12-10 | 2019-03-29 | Regents Of The University Of Minnesota | Modyfikacja DNA za pośrednictwem efektorów TAL |
US20110203012A1 (en) | 2010-01-21 | 2011-08-18 | Dotson Stanton B | Methods and compositions for use of directed recombination in plant breeding |
WO2011100058A1 (en) | 2010-02-09 | 2011-08-18 | Sangamo Biosciences, Inc. | Targeted genomic modification with partially single-stranded donor molecules |
US10087431B2 (en) | 2010-03-10 | 2018-10-02 | The Regents Of The University Of California | Methods of generating nucleic acid fragments |
EP2553122B1 (en) | 2010-03-31 | 2017-08-09 | Spartan Bioscience Inc. | Direct nucleic acid analysis |
WO2011130346A1 (en) | 2010-04-13 | 2011-10-20 | Sigma-Aldrich Co. | Methods for generating endogenously tagged protein |
ES2590343T3 (es) * | 2010-05-10 | 2016-11-21 | The Regents Of The University Of California | Composiciones de endorribonucleasas y métodos de uso de las mismas |
EP2571512B1 (en) | 2010-05-17 | 2017-08-23 | Sangamo BioSciences, Inc. | Novel dna-binding proteins and uses thereof |
US20140148361A1 (en) | 2010-06-07 | 2014-05-29 | Barry L. Stoddard | Generation and Expression of Engineered I-ONUI Endonuclease and Its Homologues and Uses Thereof |
WO2011159369A1 (en) * | 2010-06-14 | 2011-12-22 | Iowa State University Research Foundation, Inc. | Nuclease activity of tal effector and foki fusion protein |
KR20180121665A (ko) | 2010-07-23 | 2018-11-07 | 시그마-알드리치 컴퍼니., 엘엘씨 | 표적화 엔도뉴클레아제 및 단일-가닥 핵산을 사용하는 게놈 편집 |
EP2601611B1 (en) | 2010-08-02 | 2020-12-09 | Integrated Dna Technologies, Inc. | Methods for predicting stability and melting temperatures of nucleic acid duplexes |
AU2011316980A1 (en) | 2010-10-20 | 2013-04-18 | Dupont Nutrition Biosciences Aps | Lactococcus CRISPR-Cas sequences |
WO2012092259A1 (en) | 2010-12-27 | 2012-07-05 | Ibis Biosciences, Inc. | Quantitating high titer samples by digital pcr |
EP2671942B1 (en) | 2011-01-31 | 2016-09-21 | Hitachi High-Technologies Corporation | Nucleic acid test device |
KR20120096395A (ko) | 2011-02-22 | 2012-08-30 | 주식회사 툴젠 | 뉴클레아제에 의해 유전자 변형된 세포를 농축시키는 방법 |
ES2728436T3 (es) * | 2011-04-05 | 2019-10-24 | Cellectis | Método para la generación de TALE-nucleasas compactas y usos de la mismas |
US20120283109A1 (en) | 2011-04-08 | 2012-11-08 | Timothy Z. Liu | Method of analyzing target nucleic acid of biological samples |
JP6158170B2 (ja) | 2011-04-27 | 2017-07-12 | アミリス, インコーポレイテッド | ゲノム修飾のための方法 |
WO2012164565A1 (en) | 2011-06-01 | 2012-12-06 | Yeda Research And Development Co. Ltd. | Compositions and methods for downregulating prokaryotic genes |
US8927218B2 (en) | 2011-06-27 | 2015-01-06 | Flir Systems, Inc. | Methods and compositions for segregating target nucleic acid from mixed nucleic acid samples |
EP3613852A3 (en) * | 2011-07-22 | 2020-04-22 | President and Fellows of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
SG10201811736QA (en) | 2011-09-21 | 2019-02-27 | Sangamo Therapeutics Inc | Methods and compositions for regulation of transgene expression |
US8450107B1 (en) | 2011-11-30 | 2013-05-28 | The Broad Institute Inc. | Nucleotide-specific recognition sequences for designer TAL effectors |
WO2013088446A1 (en) | 2011-12-16 | 2013-06-20 | Targetgene Biotechnologies Ltd | Compositions and methods for modifying a predetermined target nucleic acid sequence |
GB201122458D0 (en) | 2011-12-30 | 2012-02-08 | Univ Wageningen | Modified cascade ribonucleoproteins and uses thereof |
US20130196323A1 (en) | 2012-02-01 | 2013-08-01 | The University Of North Carolina At Greensboro | Methods Of Nucleic Acid Analysis |
IN2014DN07853A (es) | 2012-02-24 | 2015-04-24 | Hutchinson Fred Cancer Res | |
MX359327B (es) | 2012-02-29 | 2018-09-25 | Sangamo Biosciences Inc | Composiciones y sus usos para tratar y prevenir la enfermedad de huntington. |
WO2013141680A1 (en) | 2012-03-20 | 2013-09-26 | Vilnius University | RNA-DIRECTED DNA CLEAVAGE BY THE Cas9-crRNA COMPLEX |
US9637739B2 (en) | 2012-03-20 | 2017-05-02 | Vilnius University | RNA-directed DNA cleavage by the Cas9-crRNA complex |
AU2013204327B2 (en) | 2012-04-20 | 2016-09-01 | Aviagen | Cell transfection method |
EP2841581B2 (en) | 2012-04-23 | 2023-03-08 | BASF Agricultural Solutions Seed US LLC | Targeted genome engineering in plants |
MX369788B (es) | 2012-05-02 | 2019-11-21 | Dow Agrosciences Llc | Modificacion dirigida de deshidrogenasa de malato. |
US10174331B2 (en) | 2012-05-07 | 2019-01-08 | Sangamo Therapeutics, Inc. | Methods and compositions for nuclease-mediated targeted integration of transgenes |
WO2013169398A2 (en) | 2012-05-09 | 2013-11-14 | Georgia Tech Research Corporation | Systems and methods for improving nuclease specificity and activity |
JP6343605B2 (ja) * | 2012-05-25 | 2018-06-13 | ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア | Rna依存性標的dna修飾およびrna依存性転写調節のための方法および組成物 |
WO2013181440A1 (en) | 2012-05-30 | 2013-12-05 | Baylor College Of Medicine | Supercoiled minivectors as a tool for dna repair, alteration and replacement |
WO2013188037A2 (en) | 2012-06-11 | 2013-12-19 | Agilent Technologies, Inc | Method of adaptor-dimer subtraction using a crispr cas6 protein |
US20150128300A1 (en) | 2012-06-12 | 2015-05-07 | Genentech, Inc. | Methods and compositions for generating conditional knock-out alleles |
EP2674501A1 (en) | 2012-06-14 | 2013-12-18 | Agence nationale de sécurité sanitaire de l'alimentation,de l'environnement et du travail | Method for detecting and identifying enterohemorrhagic Escherichia coli |
US9688971B2 (en) | 2012-06-15 | 2017-06-27 | The Regents Of The University Of California | Endoribonuclease and methods of use thereof |
US20150225734A1 (en) | 2012-06-19 | 2015-08-13 | Regents Of The University Of Minnesota | Gene targeting in plants using dna viruses |
WO2014011901A2 (en) | 2012-07-11 | 2014-01-16 | Sangamo Biosciences, Inc. | Methods and compositions for delivery of biologics |
EP3816281A1 (en) | 2012-07-11 | 2021-05-05 | Sangamo Therapeutics, Inc. | Methods and compositions for the treatment of lysosomal storage diseases |
ES2757623T3 (es) * | 2012-07-25 | 2020-04-29 | Broad Inst Inc | Proteínas de unión a ADN inducibles y herramientas de perturbación genómica y aplicaciones de las mismas |
WO2014022702A2 (en) | 2012-08-03 | 2014-02-06 | The Regents Of The University Of California | Methods and compositions for controlling gene expression by rna processing |
US9650648B2 (en) | 2012-08-29 | 2017-05-16 | Sangamo Biosciences, Inc. | Methods and compositions for treatment of a genetic condition |
UA118090C2 (uk) | 2012-09-07 | 2018-11-26 | ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі | Спосіб інтегрування послідовності нуклеїнової кислоти, що представляє інтерес, у ген fad2 у клітині сої та специфічний для локусу fad2 білок, що зв'язується, здатний індукувати спрямований розрив |
JP6775953B2 (ja) | 2012-09-07 | 2020-10-28 | ダウ アグロサイエンシィズ エルエルシー | Fad3性能座および標的化切断を誘導可能である対応する標的部位特異的結合タンパク質 |
UA119135C2 (uk) | 2012-09-07 | 2019-05-10 | ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі | Спосіб отримання трансгенної рослини |
EP3789405A1 (en) | 2012-10-12 | 2021-03-10 | The General Hospital Corporation | Transcription activator-like effector (tale) - lysine-specific demethylase 1 (lsd1) fusion proteins |
US20140113375A1 (en) * | 2012-10-21 | 2014-04-24 | Lixin Liu | Transient Expression And Reverse Transcription Aided Genome Alteration System |
EP3346003B1 (en) | 2012-10-23 | 2021-06-09 | Toolgen Incorporated | Composition for cleaving a target dna comprising a guide rna specific for the target dna and cas protein-encoding nucleic acid or cas protein, and use thereof |
AU2013337951B2 (en) | 2012-10-30 | 2019-10-03 | Recombinetics, Inc. | Control of sexual maturation in animals |
CA2890160A1 (en) | 2012-10-31 | 2014-05-08 | Cellectis | Coupling herbicide resistance with targeted insertion of transgenes in plants |
US20140127752A1 (en) | 2012-11-07 | 2014-05-08 | Zhaohui Zhou | Method, composition, and reagent kit for targeted genomic enrichment |
PL2928496T3 (pl) | 2012-12-06 | 2020-04-30 | Sigma-Aldrich Co. Llc | Modyfikacja i regulacja genomu w oparciu o CRISPR |
EP3031921A1 (en) | 2012-12-12 | 2016-06-15 | The Broad Institute, Inc. | Delivery, engineering and optimization of systems, methods and compositions for sequence manipulation and therapeutic applications |
AU2013359212B2 (en) | 2012-12-12 | 2017-01-19 | Massachusetts Institute Of Technology | Engineering and optimization of improved systems, methods and enzyme compositions for sequence manipulation |
EP2931892B1 (en) | 2012-12-12 | 2018-09-12 | The Broad Institute, Inc. | Methods, models, systems, and apparatus for identifying target sequences for cas enzymes or crispr-cas systems for target sequences and conveying results thereof |
US20140310830A1 (en) | 2012-12-12 | 2014-10-16 | Feng Zhang | CRISPR-Cas Nickase Systems, Methods And Compositions For Sequence Manipulation in Eukaryotes |
US8697359B1 (en) | 2012-12-12 | 2014-04-15 | The Broad Institute, Inc. | CRISPR-Cas systems and methods for altering expression of gene products |
ES2542015T3 (es) | 2012-12-12 | 2015-07-29 | The Broad Institute, Inc. | Ingeniería de sistemas, métodos y composiciones de guía optimizadas para manipulación de secuencias |
EP2931899A1 (en) * | 2012-12-12 | 2015-10-21 | The Broad Institute, Inc. | Functional genomics using crispr-cas systems, compositions, methods, knock out libraries and applications thereof |
EP2931898B1 (en) | 2012-12-12 | 2016-03-09 | The Broad Institute, Inc. | Engineering and optimization of systems, methods and compositions for sequence manipulation with functional domains |
EP4286402A3 (en) | 2012-12-12 | 2024-02-14 | The Broad Institute, Inc. | Crispr-cas component systems, methods and compositions for sequence manipulation |
DK2931897T3 (en) | 2012-12-12 | 2018-02-05 | Broad Inst Inc | CONSTRUCTION, MODIFICATION AND OPTIMIZATION OF SYSTEMS, PROCEDURES AND COMPOSITIONS FOR SEQUENCE MANIPULATION AND THERAPEUTICAL APPLICATIONS |
DK2931891T3 (da) | 2012-12-17 | 2019-08-19 | Harvard College | Rna-styret modificering af menneskelige genomer |
EP2938184B1 (en) | 2012-12-27 | 2018-10-31 | Keygene N.V. | Method for removing genetic linkage in a plant |
EP2946015B1 (en) | 2013-01-16 | 2021-05-26 | Emory University | Cas9-nucleic acid complexes and uses related thereto |
CN103233028B (zh) | 2013-01-25 | 2015-05-13 | 南京徇齐生物技术有限公司 | 一种无物种限制无生物安全性问题的真核生物基因打靶方法及螺旋结构dna序列 |
US11466306B2 (en) * | 2013-02-14 | 2022-10-11 | Osaka University | Method for isolating specific genomic regions with use of molecule capable of specifically binding to endogenous DNA sequence |
RU2018122288A (ru) | 2013-03-14 | 2019-03-06 | Карибо Биосайенсиз, Инк. | Композиции и способы с участием нуклеиновых кислот, нацеленных на нуклеиновые кислоты |
US11332719B2 (en) | 2013-03-15 | 2022-05-17 | The Broad Institute, Inc. | Recombinant virus and preparations thereof |
EP3741868B1 (en) | 2013-03-15 | 2024-05-22 | The General Hospital Corporation | Rna-guided targeting of genetic and epigenomic regulatory proteins to specific genomic loci |
US9902973B2 (en) * | 2013-04-11 | 2018-02-27 | Caribou Biosciences, Inc. | Methods of modifying a target nucleic acid with an argonaute |
CN103224947B (zh) | 2013-04-28 | 2015-06-10 | 陕西师范大学 | 一种基因打靶系统 |
EP3730615A3 (en) | 2013-05-15 | 2020-12-09 | Sangamo Therapeutics, Inc. | Methods and compositions for treatment of a genetic condition |
EP2999788B1 (en) * | 2013-05-22 | 2020-07-08 | Northwestern University | Rna-directed dna cleavage and gene editing by cas9 enzyme from neisseria meningitidis |
WO2014204725A1 (en) * | 2013-06-17 | 2014-12-24 | The Broad Institute Inc. | Optimized crispr-cas double nickase systems, methods and compositions for sequence manipulation |
CN103343120B (zh) | 2013-07-04 | 2015-03-04 | 中国科学院遗传与发育生物学研究所 | 一种小麦基因组定点改造方法 |
WO2015089473A1 (en) * | 2013-12-12 | 2015-06-18 | The Broad Institute Inc. | Engineering of systems, methods and optimized guide compositions with new architectures for sequence manipulation |
EP3080260B1 (en) | 2013-12-12 | 2019-03-06 | The Broad Institute, Inc. | Crispr-cas systems and methods for altering expression of gene products, structural information and inducible modular cas enzymes |
WO2015089364A1 (en) | 2013-12-12 | 2015-06-18 | The Broad Institute Inc. | Crystal structure of a crispr-cas system, and uses thereof |
JP6793547B2 (ja) * | 2013-12-12 | 2020-12-02 | ザ・ブロード・インスティテュート・インコーポレイテッド | 最適化機能CRISPR−Cas系による配列操作のための系、方法および組成物 |
MX2016007327A (es) | 2013-12-12 | 2017-03-06 | Broad Inst Inc | Suministro, uso y aplicaciones terapeuticas de sistemas y composiciones crispr-cas para dirigirlos a trastornos y enfermedades usando componentes para suministro de particulas. |
US11028388B2 (en) * | 2014-03-05 | 2021-06-08 | Editas Medicine, Inc. | CRISPR/Cas-related methods and compositions for treating Usher syndrome and retinitis pigmentosa |
US20150376586A1 (en) | 2014-06-25 | 2015-12-31 | Caribou Biosciences, Inc. | RNA Modification to Engineer Cas9 Activity |
US9512446B1 (en) * | 2015-08-28 | 2016-12-06 | The General Hospital Corporation | Engineered CRISPR-Cas9 nucleases |
-
2014
- 2014-03-12 RU RU2018122288A patent/RU2018122288A/ru not_active Application Discontinuation
- 2014-03-12 CA CA2905432A patent/CA2905432C/en active Active
- 2014-03-12 KR KR1020177026013A patent/KR20170108172A/ko not_active Application Discontinuation
- 2014-03-12 JP JP2016501357A patent/JP2016519652A/ja active Pending
- 2014-03-12 US US14/416,338 patent/US9260752B1/en active Active
- 2014-03-12 EP EP14771135.2A patent/EP2971167B1/en active Active
- 2014-03-12 DK DK19188624.1T patent/DK3620534T3/da active
- 2014-03-12 BR BR112015022061A patent/BR112015022061B8/pt active IP Right Grant
- 2014-03-12 CN CN201480028062.1A patent/CN105980575A/zh active Pending
- 2014-03-12 AU AU2014235794A patent/AU2014235794A1/en not_active Abandoned
- 2014-03-12 DK DK14771135T patent/DK2971167T3/da active
- 2014-03-12 ES ES19188624T patent/ES2901396T3/es active Active
- 2014-03-12 KR KR1020157028606A patent/KR101780885B1/ko active IP Right Grant
- 2014-03-12 EP EP19188624.1A patent/EP3620534B1/en active Active
- 2014-03-12 ES ES14771135T patent/ES2749624T3/es active Active
- 2014-03-12 MX MX2015013057A patent/MX2015013057A/es active IP Right Grant
- 2014-03-12 US US14/206,319 patent/US20140315985A1/en not_active Abandoned
- 2014-03-12 SG SG11201507378UA patent/SG11201507378UA/en unknown
- 2014-03-12 RU RU2015140276A patent/RU2662932C2/ru active
- 2014-03-12 CN CN202010276646.8A patent/CN111454951A/zh active Pending
- 2014-03-12 NZ NZ712727A patent/NZ712727A/en unknown
- 2014-03-12 WO PCT/US2014/023828 patent/WO2014150624A1/en active Application Filing
- 2014-03-12 GB GB1517172.1A patent/GB2527450A/en not_active Withdrawn
-
2015
- 2015-06-24 US US14/749,599 patent/US20160046949A1/en not_active Abandoned
- 2015-06-24 US US14/749,594 patent/US20160046962A1/en not_active Abandoned
- 2015-06-25 US US14/751,088 patent/US20160046963A1/en not_active Abandoned
- 2015-06-25 US US14/751,055 patent/US9410198B2/en active Active
- 2015-06-25 US US14/751,070 patent/US20160068887A1/en not_active Abandoned
- 2015-06-25 US US14/751,058 patent/US20160076020A1/en not_active Abandoned
- 2015-09-10 IL IL241563A patent/IL241563B/en active IP Right Grant
- 2015-09-21 ZA ZA2015/07006A patent/ZA201507006B/en unknown
- 2015-09-29 AU AU2015101418A patent/AU2015101418A6/en not_active Expired
- 2015-12-21 US US14/977,514 patent/US9909122B2/en active Active
-
2016
- 2016-01-29 HK HK16100993.9A patent/HK1213023A1/zh unknown
- 2016-05-19 US US15/159,619 patent/US9725714B2/en active Active
- 2016-05-19 US US15/159,776 patent/US10125361B2/en active Active
- 2016-07-05 US US15/202,518 patent/US9803194B2/en active Active
- 2016-11-04 US US15/344,487 patent/US11312953B2/en active Active
-
2017
- 2017-01-09 AU AU2017200138A patent/AU2017200138A1/en not_active Abandoned
- 2017-07-26 US US15/660,906 patent/US9809814B1/en active Active
- 2017-12-27 JP JP2017250680A patent/JP2018075026A/ja active Pending
-
2018
- 2018-02-23 US US15/904,285 patent/US20180237770A1/en active Pending
-
2021
- 2021-02-12 JP JP2021020373A patent/JP7008850B2/ja active Active
- 2021-08-20 US US17/407,928 patent/US20220282244A1/en active Pending
-
2023
- 2023-05-19 US US18/320,355 patent/US20240167023A1/en active Pending
Also Published As
Similar Documents
Publication | Publication Date | Title |
---|---|---|
GB2527450A (en) | Compositions and methods of nucleic acid-targeting nucleic acids | |
CY1121630T1 (el) | Ρυθμιση της εκφρασης γονιδιου σφαιρινης διαμεσολαβουμενη απο νουκλεασες | |
EP4234703A3 (en) | Genome engineering | |
MX2018007290A (es) | Metodos y composiciones para expresion de acido ribonucleico (arn) guia basada en polimerasa ii (pol-ii). | |
GB2544001A (en) | Methods for increasing Cas9-mediated engineering efficiency | |
WO2014033644A3 (en) | Methods of nuclease-based genetic engineering | |
EP2971041A4 (en) | USE OF RNA-GUIDED FOLI NUCLEASES (RFNS) TO INCREASE SPECIFICITY FOR RNA-LEDED MEMBERIZATION | |
NZ705742A (en) | Fad2 performance loci and corresponding target site specific binding proteins capable of inducing targeted breaks | |
MX2020010841A (es) | Composicion detergente. | |
NZ705745A (en) | Fad3 performance loci and corresponding target site specific binding proteins capable of inducing targeted breaks | |
MX2020004986A (es) | Composiciones mejoradas para tratar distrofia muscular. | |
MX2017007538A (es) | Sintesis de ácido nucleíco de volúmen pequeño, alta eficiencia. | |
EA201390586A1 (ru) | Последовательности crispr-cas lactococcus | |
MX2016011690A (es) | Edicion del genoma sin nucleasas. | |
MX2016002586A (es) | Métodos y composiciones para el tratamiento guiado por rna de infección por hiv. | |
NZ631512A (en) | Compositions and methods for modulating apolipoprotein (a) expression | |
BR112017011510A2 (pt) | edição de rna direcionado | |
MX2021014663A (es) | Il-23a y tnf-alfa orientados y compuesto y sus usos. | |
EP3074520A4 (en) | Selective amplification of nucleic acid sequences | |
NZ740630A (en) | Polymethine compounds and their use as fluorescent labels | |
MX359291B (es) | Adn libre de celulas como objetivo terapeutico para la infertilidad femenina y marcador de diagnostico. | |
SI3581654T1 (sl) | Postopki in sestavki za specifično inhibicijo glikolat-oksidaze (HA01) z dvoverižno RNA | |
IN2015DN04148A (es) | ||
MX363880B (es) | Sondas, cebadores cooperativos y aplicaciones de ellos. | |
PH12016501170A1 (en) | Rnapii-140 nucleic acid molecules that confer resistance to coleopteran pests |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG | Grant or registration |