Berglund, 2021 - Google Patents
Analyzing binding motifs for WW, MATH, and MAGE domains using Proteomic Peptide Phage DisplayBerglund, 2021
View PDF- Document ID
- 3869868968803941160
- Author
- Berglund S
- Publication year
External Links
Snippet
E3 ligases are responsible for binding and ubiquitinating proteins, marking them for degradation in a cell. Their binding specificity determines which proteins get degraded and is crucial both in normal cell function and in disease. In this project we find binding motifs for …
- 108090000765 processed proteins & peptides 0 title abstract description 24
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by the preceding groups
- G01N33/48—Investigating or analysing materials by specific methods not covered by the preceding groups biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
- C07K2319/41—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation containing a Myc-tag
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US20220315628A1 (en) | Amino acid-specific binder and selectively identifying an amino acid | |
Case et al. | The directional preference of kinesin motors is specified by an element outside of the motor catalytic domain | |
Kohler et al. | YidC and Oxa1 form dimeric insertion pores on the translating ribosome | |
EP2882844A2 (en) | Protease-resistant systems for polypeptide display and methods of making and using thereof | |
CA2607104A1 (en) | Sequence enabled reassembly (seer) - a novel method for visualizing specific dna sequences | |
US20220025003A1 (en) | Reagents and methods for controlling protein function and interaction | |
US20240101615A1 (en) | Orthogonal protein heterodimers | |
EP4157854A2 (en) | Modular and generalizable biosensor platform based on de novo designed protein switches | |
Berglund | Analyzing binding motifs for WW, MATH, and MAGE domains using Proteomic Peptide Phage Display | |
US20160229899A1 (en) | LUCIGEN YELLOW (LucY), A YELLOW FLUORESCENT PROTEIN | |
Xun et al. | Cell-free synthesis and combinatorial selective 15N-labeling of the cytotoxic protein amoebapore A from Entamoeba histolytica | |
CA2785359C (en) | Protein display | |
Raychaudhury et al. | Structure and function of interacting IcmR-IcmQ domains from a type IVb secretion system in Legionella pneumophila | |
US20230296616A1 (en) | Arginine fluorescent probe, preparation method therefor and application thereof | |
Zhang et al. | Expression and purification of soluble human cystatin C in Escherichia coli with maltose-binding protein as a soluble partner | |
JP2017530726A (en) | Antibody-like protein | |
US20090111968A1 (en) | Self-assembled proteins and related methods and protein structures | |
US20240228575A9 (en) | Allosteric coupling of antibody and naturally switchable, multi-subunit output protein | |
CN113817067B (en) | Cyclodiguanylate optical probe and preparation method and application thereof | |
Izquierdo-Martinez et al. | DipM controls multiple autolysins and mediates two regulatory feedback loops promoting cell constriction in C. crescentus | |
Wong et al. | Development of cell-free platforms for discovering, characterizing, and engineering post-translational modifications | |
Tyrosine | Check for updates Chapter 16 Engineering of SH2 Domains for the Recognition of Protein Tyrosine O-Sulfation Sites Sean Paul Waldrop, Wei Niu, and Jiantao Guo | |
Yamabhai | BAP-fusion: A versatile molecular probe for biotechnology research | |
McClymont | Methods to identify novel α-helical peptides that bind to LMO4 | |
Moseley | Regulation of actin dynamics by Saccharomyces cerevisiae formins and twinfilin |