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El-Sagheer et al., 2017 - Google Patents

Single tube gene synthesis by phosphoramidate chemical ligation

El-Sagheer et al., 2017

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Document ID
3661305955149502403
Author
El-Sagheer A
Brown T
Publication year
Publication venue
Chemical Communications

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Snippet

Templated chemical ligation of 5′-amino and 3′-phosphate oligonucleotides was used to synthesise a 762 base pair gene for green fluorescent protein. The phosphoramidate linkage can be read by DNA polymerase and transcribed to make RNA. We also show that …
Continue reading at ora.ox.ac.uk (PDF) (other versions)

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
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