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Mate et al., 2017 - Google Patents

The pocket manual of directed evolution: tips and tricks

Mate et al., 2017

Document ID
3373821469164625903
Author
Mate D
Gonzalez-Perez D
Mateljak I
de Santos P
Vicente A
Alcalde M
Publication year
Publication venue
Biotechnology of Microbial Enzymes

External Links

Snippet

Directed evolution is a robust approach to tailor enzymes with improved attributes. One round of laboratory evolution comprises three essential steps:(1) the generation of diversity by random mutagenesis and/or DNA recombination of the parental genes,(2) cloning and …
Continue reading at www.sciencedirect.com (other versions)

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
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