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Spinck et al., 2023 - Google Patents

Genetically programmed cell-based synthesis of non-natural peptide and depsipeptide macrocycles

Spinck et al., 2023

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Document ID
2747779411311970819
Author
Spinck M
Piedrafita C
Robertson W
Elliott T
Cervettini D
de la Torre D
Chin J
Publication year
Publication venue
Nature chemistry

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The direct genetically encoded cell-based synthesis of non-natural peptide and depsipeptide macrocycles is an outstanding challenge. Here we programme the encoded synthesis of 25 diverse non-natural macrocyclic peptides, each containing two non …
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1062—Isolating an individual clone by screening libraries mRNA-Display, e.g. polypeptide and encoding template are connected covalently
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    • C12N15/09—Recombinant DNA-technology
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