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Quassinti et al., 1998 - Google Patents

Purification of swine serum angiotensin converting enzyme with high recovery of activity using lisinopril coupled to epoxy‐activated sepharose 6B

Quassinti et al., 1998

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Document ID
17124037802687738603
Author
Quassinti L
Miano A
Bramucci M
Maccari E
Amici D
Publication year
Publication venue
IUBMB Life

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Snippet

The Authors describe the purification of swine serum ACE to molecular homogeneity with high recovery of activity (40%) in a few stepsl. The purification procedure consists of affinity chromatography, using commercial activated resin (epoxy‐activated sepharose 6B) and two …
Continue reading at iubmb.onlinelibrary.wiley.com (PDF) (other versions)

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases Endopeptidases (3.4.21-3.4.25)

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