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Marcum et al., 1986 - Google Patents

Cloned bovine aortic endothelial cells synthesize anticoagulantly active heparan sulfate proteoglycan.

Marcum et al., 1986

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Document ID
15170884124135615489
Author
Marcum J
Atha D
Fritze L
Nawroth P
Stern D
Rosenberg R
Publication year
Publication venue
Journal of Biological Chemistry

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Cloned bovine aortic endothelial cells were cultured with [35S] Na2SO4 and proteolyzed extensively with papain. Radiolabeled heparan sulfate was isolated by DEAE-Sephacel chromatography. The mucopolysaccharide was then affinity fractionated into two separate …
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)

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