Marcum et al., 1986 - Google Patents
Cloned bovine aortic endothelial cells synthesize anticoagulantly active heparan sulfate proteoglycan.Marcum et al., 1986
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- 15170884124135615489
- Author
- Marcum J
- Atha D
- Fritze L
- Nawroth P
- Stern D
- Rosenberg R
- Publication year
- Publication venue
- Journal of Biological Chemistry
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Snippet
Cloned bovine aortic endothelial cells were cultured with [35S] Na2SO4 and proteolyzed extensively with papain. Radiolabeled heparan sulfate was isolated by DEAE-Sephacel chromatography. The mucopolysaccharide was then affinity fractionated into two separate …
- 229920002971 Heparan sulfate 0 title abstract description 96
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
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