Kim et al., 1998 - Google Patents
Characterization of chimeric antibody producing CHO cells in the course of dihydrofolate reductase‐mediated gene amplification and their stability in the absence of …Kim et al., 1998
- Document ID
- 13532644644594006518
- Author
- Kim S
- Kim N
- Ryu C
- Hong H
- Lee G
- Publication year
- Publication venue
- Biotechnology and bioengineering
External Links
Snippet
Recombinant Chinese hamster ovary (CHO) cells expressing a high‐level of chimeric antibody against S surface antigen of hepatitis B virus were obtained by co‐transfection of heavy and light chain cDNA expression vectors into dihydrofolate reductase (dhfr)‐deficient …
- 102000004965 antibodies 0 title abstract description 46
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material Not used, see subgroups
- C12N5/12—Fused cells, e.g. hybridomas
- C12N5/16—Animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues ; Not used, see subgroups
- C12N5/0602—Vertebrate cells
- C12N5/0693—Tumour cells; Cancer cells
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Kim et al. | Characterization of chimeric antibody producing CHO cells in the course of dihydrofolate reductase‐mediated gene amplification and their stability in the absence of selective pressure | |
| US11447560B2 (en) | Methods of synthesizing heteromultimeric polypeptides in yeast using a haploid mating strategy | |
| US7429380B2 (en) | Production of a multimeric protein by cell fusion method | |
| US6207418B1 (en) | Production of a multimeric protein by cell fusion method | |
| Geisse et al. | Eukaryotic expression systems: a comparison | |
| US6303313B1 (en) | Method for generating libraries of antibody genes comprising amplification of diverse antibody DNAs and methods for using these libraries for the production of diverse antigen combining molecules | |
| Strutzenberger et al. | Changes during subclone development and ageing of human antibody-producing recombinant CHO cells | |
| TWI221483B (en) | Method for producing recombinant cell line featuring increased expression of amino acid sequence | |
| JPH0789909B2 (en) | Manufacturing method of hybridoma cell line | |
| Yoshikawa et al. | Flow cytometry: an improved method for the selection of highly productive gene‐amplified CHO cells using flow cytometry | |
| Dorai et al. | Correlation of heavy and light chain mRNA copy numbers to antibody productivity in mouse myeloma production cell lines | |
| Kim et al. | Decreased chimeric antibody productivity of KR12H‐1 transfectoma during long‐term culture results from decreased antibody gene copy number | |
| Bae et al. | Stability of transfectomas producing chimeric antibody against the pre‐S2 surface antigen of hepatitis B virus during a long‐term culture | |
| JP3449418B2 (en) | Increased expression by gene targeting into endogenous retrovirus-like sequences | |
| US20040053363A1 (en) | Method for production of a multimeric protein by cell fusion | |
| US20040053359A1 (en) | Method for production of a multimeric protein by cell fusion | |
| GB2237288A (en) | Amplification of a heterologous gene in recombinant eukaryotic host cells | |
| AU768101B2 (en) | Production of a multimeric protein by cell fusion method | |
| Lee et al. | Stability of Chimeric Antibody Producing Transfectomas during a Long-Term Culture | |
| KIM et al. | Department of Biological Sciences, KAIST, Taejon 305-701, Korea 1 Antibody Engineering Research Group, KRIBB, Taejon 305-606, Korea |