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Morgan et al., 1986 - Google Patents

Optimal parameters for the polybrene-induced DNA transfection of diploid human fibroblasts

Morgan et al., 1986

Document ID
13028911495981612653
Author
Morgan T
Maher V
Justin McCormick J
Publication year
Publication venue
In vitro cellular & developmental biology

External Links

Snippet

Recently it has been shown that Polybrene, in conjuction with dimethyl sulfoxide (DMSO) shock, can markedly increase frequency of DNA transfection of chicken embryo fibroblasts as compared with the frequency obtained with the standard calcium phosphate protocol. We …
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues ; Not used, see subgroups
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0623—Stem cells
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues ; Not used, see subgroups
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    • C12N5/0603—Embryonic cells ; Embryoid bodies
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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