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Wanker et al., 1991 - Google Patents

Expression of the Bacillus subtilis levanase gene in Escherichia coli and Saccharomyces cerevisiae

Wanker et al., 1991

Document ID
1105553753281887341
Author
Wanker E
Schörgendorfer K
Schwab H
Publication year
Publication venue
Journal of biotechnology

External Links

Snippet

The gene coding for the inulin hydrolyzing enzyme levanase which was previously cloned from Bacillus subtilis was fused to the tac-promoter. Overexpression in Escherichia coli resulted in high amounts of intracellularly produced levanase (up to 20 U mg− 1. After …
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    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
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    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
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    • C12N9/10—Transferases (2.)
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