Golshadi et al., 2016 - Google Patents
High‐Efficiency Gene Transfection of Cells through Carbon Nanotube ArraysGolshadi et al., 2016
View PDF- Document ID
- 10747659717558287988
- Author
- Golshadi M
- Wright L
- Dickerson I
- Schrlau M
- Publication year
- Publication venue
- Small
External Links
Snippet
Introducing nucleic acids into mammalian cells is a crucial step to elucidate biochemical pathways, and to modify gene expression and cellular development in immortalized cells, primary cells, and stem cells. Current transfection technologies are time consuming and …
- 239000002041 carbon nanotube 0 title abstract description 86
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by the preceding groups
- G01N33/48—Investigating or analysing materials by specific methods not covered by the preceding groups biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions involving viable micro-organisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Golshadi et al. | High‐Efficiency Gene Transfection of Cells through Carbon Nanotube Arrays | |
Brandenberg et al. | High-throughput automated organoid culture via stem-cell aggregation in microcavity arrays | |
Li et al. | Transparent and Gas‐Permeable Liquid Marbles for Culturing and Drug Sensitivity Test of Tumor Spheroids | |
Oomen et al. | Chemical analysis of single cells | |
Staab et al. | Co‐culture system of human enteroids/colonoids with innate immune cells | |
McKnight et al. | Tracking gene expression after DNA delivery using spatially indexed nanofiber arrays | |
Mumm et al. | A Transparent Nanowire‐Based Cell Impalement Device Suitable for Detailed Cell–Nanowire Interaction Studies | |
CN113767176A (en) | Imaging system hardware | |
Wang et al. | A nanostructured molybdenum disulfide film for promoting neural stem cell neuronal differentiation: toward a nerve tissue‐engineered 3D scaffold | |
Tu et al. | A microfluidic chip for cell patterning utilizing paired microwells and protein patterns | |
US20230183786A1 (en) | Methods for identifying and modulating co-occurant cellular phenotypes | |
Yamauchi et al. | Spatially and temporally controlled gene transfer by electroporation into adherent cells on plasmid DNA-loaded electrodes | |
Mukherjee et al. | Deep Learning‐Assisted Automated Single Cell Electroporation Platform for Effective Genetic Manipulation of Hard‐to‐Transfect Cells | |
Kim et al. | Vertically Coated Graphene Oxide Micro‐Well Arrays for Highly Efficient Cancer Spheroid Formation and Drug Screening | |
Taskin et al. | Combined cell culture-biosensing platform using vertically aligned patterned peptide nanofibers for cellular studies | |
Liu et al. | Reconstructing soma–soma synapse-like vesicular exocytosis with DNA origami | |
Patino et al. | High-throughput microfluidics platform for intracellular delivery and sampling of biomolecules from live cells | |
Fletcher et al. | Microarrays of biomimetic cells formed by the controlled synthesis of carbon nanofiber membranes | |
CN105950467A (en) | Method for detecting cytotoxicity of to-be-tested medicine to target cell and cell chip specially used by method | |
Kim et al. | CRISPR/CAS-Assisted Nanoneedle sensor for adenosine triphosphate detection in living cells | |
EP3356044B1 (en) | Delivery of biomolecules into cells through carbon nanotube arrays | |
Li et al. | A simple microsphere‐based mold to rapidly fabricate microwell arrays for multisize 3D tumor culture | |
Zhang et al. | Interplay between intracellular transport dynamics and liquid‒liquid phase separation | |
Ye et al. | Fabrication of poly HEMA grids by micromolding in capillaries for cell patterning and single‐cell arrays | |
Sun et al. | A Sessile Drop Method for Facile and Robust Spheroid Cultures |