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Lo et al., 2005 - Google Patents

Asymmetrical synthesis of l‐homophenylalanine using engineered Escherichia coli aspartate aminotransferase

Lo et al., 2005

Document ID
6676119990498238035
Author
Lo H
Hsu S
Lin W
Chan N
Hsu W
Publication year
Publication venue
Biotechnology progress

External Links

Snippet

Site‐directed mutagenesis was performed to change the substrate specificity of Escherichia coli aspartate aminotransferase (AAT). A double mutant, R292E/L18H, with a 12.9‐fold increase in the specific activity toward l‐lysine and 2‐oxo‐4‐phenylbutanoic acid (OPBA) …
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    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/22—Tryptophan; Tyrosine; Phenylalanine; 3,4-Dihydroxyphenylalanine
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    • C12P41/006—Processes using enzymes or micro-organisms to separate optical isomers from a racemic mixture by reactions involving C-N bonds, e.g. nitriles, amides, hydantoins, carbamates, lactames, transamination reactions, or keto group formation from racemic mixtures
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