Nothing Special   »   [go: up one dir, main page]

Nag et al., 1979 - Google Patents

Dissociation of adult mammalian heart into single cell suspension: an ultrastructural study

Nag et al., 1979

View PDF
Document ID
4514667480542843379
Author
Nag A
Zak R
Publication year
Publication venue
Journal of anatomy

External Links

Snippet

Adult rat heart was dissociated into a single cell suspension by a perfusion technique which used 0.05% collagenase and 0.1% hyaluronidase in Krebs-Ringer phosphate buffer (KRP). The non-muscle cells of the suspension were separated from the myocytes by centrifugation …
Continue reading at pmc.ncbi.nlm.nih.gov (PDF) (other versions)

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues ; Not used, see subgroups
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues ; Not used, see subgroups
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues ; Not used, see subgroups
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/30—Nerves; Brain; Eyes; Corneal cells; Cerebrospinal fluid; Neuronal stem cells; Neuronal precursor cells; Glial cells; Oligodendrocytes; Schwann cells; Astroglia; Astrocytes; Choroid plexus; Spinal cord tissue
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/44—Vessels; Vascular smooth muscle cells; Endothelial cells; Endothelial progenitor cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/37—Digestive system

Similar Documents

Publication Publication Date Title
Nag et al. Dissociation of adult mammalian heart into single cell suspension: an ultrastructural study
Lernmark The preparation of, and studies on, free cell suspensions from mouse pancreatic islets
Kinlough-Rathbone et al. Properties of washed human platelets
Munthe-Kaas et al. Mass isolation and culture of rat kupffer cells.
Mills et al. Electron microscopic study of isolated Kupffer cells
Bischoff Enzymatic liberation of myogenic cells from adult rat muscle
Cohn et al. The differentiation of mononuclear phagocytes Morphology, cytochemistry, and biochemistry
Steinman et al. Identification of a novel cell type in peripheral lymphoid organs of mice: I. Morphology, quantitation, tissue distribution
Piper et al. Culturing of calcium stable adult cardiac myocytes
Khalid et al. Direct detection of endogenous hydroxyl radical production in cultured adult cardiomyocytes during anoxia and reoxygenation. Is the hydroxyl radical really the most damaging radical species?
Björkman et al. The cell types in the endocrine pancreas of the human fetus
Simionescu et al. Isolation and characterization of endothelial cells from the heart microvasculature
Robinson et al. Unusual lysosomes in aortic smooth muscle cells: presence in living and rapidly frozen cells.
Vorbrodt et al. Ultrastructural localization of lectin receptors on the luminal and abluminal aspects of brain micro-blood vessels.
Piper et al. Microvascular endothelial cells from heart
Vaughan et al. Studies of proliferative capabilities in isolated epidermal basal and differentiated cells
Schulitz et al. Survival of satellie cells in whole muscle transplants
Fry et al. The ultrastructure of membrane alterations of enzymatically dissociated cardiac myocytes
Masuko et al. Isolation and culture of motoneurons from embryonic chicken spinal cords.
Burghen et al. Factors influencing isolation of islets of Langerhans
Poduslo et al. The bulk separation of neuroglia and neuron perikarya
Pretlow et al. Separation of beating cardiac myocytes from suspensions of heart cells
Himmelhoch et al. Caenorhabditis briggsae: aging and the structural turnover of the outer cuticle surface and the intestine
Nopanitaya et al. New observations on the fine structure of the liver in goldfish (Carassius auratus)
Helfrich et al. Osteoclast generation from human fetal bone marrow in cocultures with murine fetal long bones: a model for in vitro study of human osteoclast formation and function