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Yang et al., 2007 - Google Patents

Fed‐batch bioreactor process scale‐up from 3‐L to 2,500‐L scale for monoclonal antibody production from cell culture

Yang et al., 2007

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Document ID
4401255909926205370
Author
Yang J
Lu C
Stasny B
Henley J
Guinto W
Gonzalez C
Gleason J
Fung M
Collopy B
Benjamino M
Gangi J
Hanson M
Ille E
Publication year
Publication venue
Biotechnology and bioengineering

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Snippet

This case study focuses on the scale‐up of a Sp2/0 mouse myeloma cell line based fed‐ batch bioreactor process, from the initial 3‐L bench scale to the 2,500‐L scale. A stepwise scale‐up strategy that involved several intermediate steps in increasing the bioreactor …
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    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
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    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
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    • C12P21/005—Glycopeptides, glycoproteins
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    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48—Automatic or computerized control
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    • C12N2510/00—Genetically modified cells
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