IMPLEN N50-Go Nano Photo Meter User Manual

Zamkatimu kubisa

1 IMPLEN N50-Go Nano Photo Meter

2 Zambiri Zamalonda

3 Malangizo Ogwiritsira Ntchito Zogulitsa

4 Gulu la chitetezo IP20

10 Zolemba / Zothandizira

10.1 Maumboni

IMPLEN N50-Go Nano Photo Meter

Zambiri Zamalonda

Chogulitsacho ndi spectrophotometer yokhala ndi pulogalamu ya 4.6.16350 ndi buku la 4.6.2. Amapangidwa ndi Implen, Inc. ndipo amabwera ndi chithandizo cha telefoni chopezeka kwa makasitomala ku North ndi South America. Zogulitsazo zimakumana ndi zotetezedwa zosiyanasiyana komanso zachilengedwe, kuphatikiza Low Voltage
Equipment Safety Directive, Electromagnetic compatibility (EMC) malangizo, Zoletsa pakugwiritsa ntchito Zinthu Zowopsa mu Zida Zamagetsi ndi Zamagetsi (ROHS), ndi EC Directive on Waste Electrical and Electronic Equipment (WEEE).

Malangizo Ogwiritsira Ntchito Zogulitsa

Zida: Chogulitsacho chimabwera ndi zowonjezera komanso zosafunikira. Onani buku la ogwiritsa ntchito kuti mumve zambiri za zowonjezera.

Kuyambapo:
- Kuyika kwa Spectrophotometer: Tsatirani malangizo omwe ali m'buku la ogwiritsa ntchito kuti muyike bwino spectrophotometer.
- Zambiri Zachitetezo: Werengani gawo la chidziwitso cha chitetezo mu bukhu la ogwiritsa ntchito musanagwiritse ntchito malonda.
- Kutulutsa ndi Kuyika: Tsatirani malangizo omwe ali m'buku la ogwiritsa ntchito kuti mutulutse bwino ndikuyika chinthucho.

Zithunzi: Onani gawo la zithunzi mu bukhu la ogwiritsa ntchito kuti mumvetsetse tanthauzo la zithunzi zosiyanasiyana zomwe zikuwonetsedwa pazenera.
Mabatani: Onani gawo la mabatani mu bukhu la ogwiritsa ntchito kuti mumvetsetse ntchito ya mabatani osiyanasiyana pa malonda.

Zowonetsera zoyezera: Onani gawo la zowonera mu bukhu la ogwiritsa ntchito kuti mumvetsetse momwe mungawerenge ndikutanthauzira masikirini osiyanasiyana omwe amawonetsedwa pazogulitsa.
Njira: Onani gawo la njira mu bukhu la ogwiritsa ntchito kuti mumvetsetse momwe mungagwiritsire ntchito njira zosiyanasiyana zoyezera samposagwiritsa ntchito mankhwala.

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NanoPhotometer® N50-Go/C40-Go
Buku Logwiritsa Ntchito
Mtundu wa 4.6.2 wa Mapulogalamu a 4.6.16350

NanoPhotometer® N50-Go/C40-Go User Manual Version 4.6.2 Wogwiritsa ntchito NanoPhotometer® Go (“End User”) potero amatenga udindo wonse wosunga zotetezedwa ndi zosunga zobwezeretsera zonse. files ndi/kapena deta yomwe ingapangidwe, kusungidwa kapena kusamutsidwa kuchokera ku chipangizocho. Wogwiritsa ntchito amavomereza kuti ndizotheka kuti deta ndi/kapena files zitha kutayika kapena kuwonongeka, ndipo amavomereza ndikuvomereza kuti ali ndi udindo wosunga zosunga zobwezeretsera zonse zoyenera. files ndi data. Pogwiritsa ntchito chipangizo cha NanoPhotometer® Go, Wogwiritsa Ntchito Pamapeto akuvomereza izi, ndipo akuvomereza kuti Implen sidzakhala ndi mlandu pakutayika, kuchotsa kapena kuwonongeka kwa deta iliyonse kapena files pazifukwa zilizonse, kuphatikiza kuwonongeka kulikonse komwe kumabwera chifukwa cha izi.
Thandizo la foni likupezeka pogwiritsa ntchito imodzi mwa manambala a foni awa ochokera kudera lanu: North ndi South America
Foni: +1 818 748 6400 Fax: +1 818 449-0416 Imelo: info@implen-go.com Webmalo: www.implen-go.com Implen, Inc. Unit 104 31194 La Baya Drive Westlake Village, CA 91362 USA
Windows ndi Excel ndi zizindikilo za Microsoft Corporation Redmond, WA macOS ndi chizindikiro cha Apple Inc. Cupertino, CA
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NanoPhotometer® N50-Go/C40-Go User Manual Version 4.6.2

Chilengezo chogwirizana ndi NanoPhotometer® (C40-Go/N50Go)

Uku ndikutsimikizira kuti Implen NanoPhotometer® Go ikugwirizana ndi zofunikira za malangizo awa:

2014/35/EU

Kutsika Voltage Equipment Safety Directive

2014/30/EU

Electromagnetic compatibility (EMC) malangizo

IEC 60529

Gulu la chitetezo IP20

2011/65/EU

Zoletsa pakugwiritsa ntchito Zinthu Zina Zowopsa mu Zida Zamagetsi ndi Zamagetsi (ROHS)

2012/19/EU

EC Directive on Waste Electrical and Electronic Equipment (WEEE) 2003/108/EC & 2008/34/EC. Powonetsetsa kuti mankhwalawa atayidwa moyenera, muthandizira kupewa zotsatira zoyipa zomwe zingachitike ku chilengedwe komanso thanzi la anthu, zomwe zitha kuchitika chifukwa chogwiritsa ntchito zinyalala mosayenera.

FCC 47 CFR Gawo15 §15.107 ndi §15.109

EN 301 489-1 V1.9.2

Wailesi ndi zida zothandizira kuti zigwiritsidwe ntchito kunyamula (zida zonyamula); EUT Ntchito pafupipafupi osiyanasiyana: 2.4 2.4835 GHz

EN 300 328 V1.8.1

Kugwirizana kwa Electromagnetic ndi Radio spectrum Matters (ERM); Wideband kufala machitidwe; Zida zotumizira deta zomwe zikugwira ntchito mu bandi ya 2,4 GHz ISM ndikugwiritsa ntchito njira zosinthira bandi; EN Yogwirizana yomwe ikukhudza zofunikira za Article 3.2 ya R&TTE Directive

EN 301 489-17 V2.2.1

Kugwirizana kwa Electromagnetic ndi Radio Spectrum Matters (ERM)

Miyezo yomwe imalengezedwa, ngati kuli koyenera, ndi motere:

IEC/EN 61010-1:2012 EN61326-1:2013

Zofunikira pachitetezo pazida zamagetsi poyeza, kuwongolera, ndikugwiritsa ntchito labotale. Zofunikira zonse.
Electromagnetic comppatibility- generic emission standard standard zida zamagetsi zoyezera, kuwongolera, ndikugwiritsa ntchito labotale.

Sayinidwa:

Dr. Thomas Sahiri Managing Director Implen GmbH

NanoPhotometer® N50-Go/C40-Go User Manual Version 4.6.2
NANOPHOTOMETER® N50-GO
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NanoPhotometer® N50-Go/C40-Go User Manual Version 4.6.2
NANOPHOTOMETER® C40-GO
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NanoPhotometer® N50-Go/C40-Go User Manual Version 4.6.2
CHIKWANGWANI CHAKUM'MBUYO PANEL
Kuti muyambitse/kuzimitsa NanoPhotometer® Go kankhani posachedwa (<1 sekondi imodzi) pa batani lamphamvu loyatsa/kuzimitsa kumbuyo kwa NanoPhotometer® Go. Zindikirani: Kukankhira kutali (> 3 masekondi) kumayambitsa kukonzanso mwamphamvu. Ingoyambitsani kukonzanso mwamphamvu kwa NanoPhotometer® Go pakafunika. Kuti mupewe kuyambiranso kolimba kosafunikira, tikulimbikitsidwa kuti mutsitse chipangizocho kuchokera pazenera loyang'ana podina batani lamphamvu ( ) pansi pakona yakumanzere kwa chophimba chakunyumba.
CHIYAMBI CHONSE VIEW
Dzina lachitsanzo, nambala ya serial ya chipangizo ndi ID ya FCC zili pa mbale yozindikiritsa pansi pa chidacho.
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NanoPhotometer® N50-Go/C40-Go User Manual Version 4.6.2

ZAMBIRI

STANDARD ACCESSORIES
Cholumikizira Chingwe
Chingwe cholumikizira cha USB kuti mulumikize NanoPhotometer® Pitani pakompyuta kuti muwongolere NanoPhotometer® Pitani kudzera pa kompyuta (chonde onani tsamba 21 Kuyika Mapulogalamu).
NanoPhotometer® Go Power Adapter
Adaputala yamagetsi ya NanoPhotometer® Go. Zindikirani: Gwiritsani ntchito adaputala yamagetsi yokhayo yomwe yaperekedwa ndi chida chanu kapena cholowa chochokera kwa wopanga kapena wopereka wanu.
Chophimba cha Fumbi
ZOCHITIKA ZOKHUDZA
Sefa ya Galasi ya Didymium (C40-Go) Sefa yamagalasi ya didymium yotsimikizika itha kugwiritsidwa ntchito kutsimikizira kutalika kwa mafunde ndi zithunzi za makina a NanoPhotometer® C40-Go cuvette.
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NanoPhotometer® N50-Go/C40-Go User Manual Version 4.6.2
Standard Solution (N50-Go)
NanoPhotometer® Go ndi yaulere chifukwa chake sikofunikira kuyambiransoview kulondola kwa photometric pafupipafupi. Ngati ma SOP a labotale amafuna kuwongolera mwachizolowezi mayankho amtundu wa photometric angagwiritsidwe ntchito.

Zindikirani: Mafotokozedwe a yankho lokhazikika amatsimikizika kwa chaka chimodzi. Chonde onani tsiku lotha ntchito. Chophimba chikatsegulidwa, chikhoza kugwiritsidwa ntchito kwa mphindi 30. Zindikirani: Chonde werengani Material Safety Data Sheet mosamala musanagwiritse ntchito mankhwalawa.

DiluCellTM (C40-Go)
DiluCell TM ndi cuvette yotayika yokhala ndi njira yofupikitsa yochepetsera pafupifupi ma cuvette-based s.amples. Chifukwa chafupikitsa njira kutalika DiluCell TM amapereka dilution basi popanda kufunika kwa thupi dilution apamwamba ndende s.amples. Pali mitundu iwiri yosiyana ya DiluCells yomwe ilipo DC 10 ndi DC 20 yomwe imalola kuti 1/10 ndi 1/20 dilution ya s ikhale yosiyana.ample motero. Kulambalala buku sample dilutions amachepetsa zolakwa dilution ndi mtanda kuipitsidwa kupanga DiluCellTM abwino kwa GLP. Kuphatikizidwa ndi ang'onoang'ono sampZofunikira za voliyumu ndi kudzaza kwaulere, DiluCell TM imalola kusanthula kosavuta kwa spectrophotometric kuchokera ku 340 - 950 nm.

Wowerenga Barcode
Ndizotheka kuitanitsa sampmayina kuchokera ku 1D ndi 2D barcode. Lumikizani chowerengera cha barcode chogwirizana ndi doko la USB la NanoPhotometer® Go ndikukankhira pa sampzenera lolowetsa dzina. Pambuyo pofufuza barcode ndi sample name adzawonetsedwa pawindo lolowera. Dzina lochokera kunja likhoza kusinthidwa kapena kusinthidwa kwathunthu.

Owerenga barcode omwe ayesedwa ndikutsimikiziridwa kuti amagwirizana:

1D:

Honeywell Voyager 95X0 Single-Line Laser Scanner

Datalogic Touch65

1D & 2D: AGPtEK SC36

Honeywell Xenon 1900g

DYMO Label Printer Ndizotheka kulumikiza chosindikizira cha DYMO Label ku NanoPhotometer® Pitani kuti musindikize mwachindunji pamalebulo okhazikika kapena a cryo. Osindikiza ovomerezeka ndi oyesedwa ndi DYMO LabelWriter 4XL/5XL (label size 10.3 x 15.8 cm) ndi DYMO LabelWriter 450/550 (5.4 x 10 cm).
Zolemba za Cryo zitha kusindikizidwa ndi chosindikizira cha DYMO Label 4XL ndi 450 pogwiritsa ntchito mawonekedwe otsatirawa: 26 x 12.7 mm ndi bwalo la 9.5 mm (malo).

NanoPhotometer® N50-Go/C40-Go User Manual Version 4.6.2 Zindikirani: Pepala lolemba la Cryo silikupezeka/logwirizana ndi chosindikizira cha DYMO Label 5XL ndi 550. Zindikirani: Mukayamba NanoPhotometer® Go, lowetsani chosindikizira cha DYMO. Chophimba chakunyumba chidzawonetsedwa. Dikirani osachepera 30 masekondi kuti dalaivala kukhazikitsa. Zolemba za Cryo (26 x 12.7 mm ndi bwalo la 9.5 mm):
Chidziwitso: Kuyika kwa zilembo za cryo pa zojambula zonyamulira kuyenera kukhala kowoneka bwino.

Kusindikiza kwa DYMO LabelWriter 4XL/5XL

Kusindikiza kwa DYMO LabelWriter 450/550

Chidziwitso: Zosindikiza zimakongoletsedwa ndi kukula kwa zilembo za DYMO LabelWriter 4XL. Osindikiza ena onse a DYMO atha kugwiritsidwa ntchito. Komabe, kukula kwa mafonti kudzasinthidwa kukhala kukula kwa pepala.

NanoPhotometer® N50-Go/C40-Go User Manual Version 4.6.2

HP Printer
Kusindikiza kuchokera ku NanoPhotometer® Go ndikotheka kudzera pa USB (HP osindikiza) komanso kudzera pa intaneti. Kusindikiza kwa netiweki kuyenera kukhala kotheka ndi osindikiza a AirPrint / IPP omwe amathandizira mtundu wa PDF. Zokonda pa printer ya netiweki onani tsamba 96 Network Printer.

Zindikirani: IPP version 2.2 ndiyofunika ndipo makonda ena osindikizira angafunikire kusinthidwa kuti athe kulumikizana ndi NanoPhotometer® Go.

Osindikiza a HP otsatirawa ayesedwa ndipo awonedwa kuti ndi ogwirizana kuti asindikizidwe kudzera pa USB

kulumikizana:

HP LaserJet 3030

HP DeskJet 2543

HP LaserJet m1522nf MFP

HP DeskJet 1110

HP LaserJet 400 mtundu M451nw

Osindikiza ena a HP amapezeka mukawapempha.

Chidziwitso: Mukayamba NanoPhotometer® Go, lowetsani chosindikizira cha HP. Chophimba chakunyumba chidzawonetsedwa. Dikirani osachepera 30 masekondi kuti dalaivala kukhazikitsa.

KULUMIKIZANA

USB A
Pali doko la USB A kutsogolo komanso gulu lakumbuyo la NanoPhotometer® Go lomwe limagwirizana ndi zida zosungirako za USB 2.0 (kumbuyo) ndi USB 3.0 (kutsogolo) kusamutsa deta mwachindunji m'mitundu yosiyanasiyana kuphatikiza Excel. . Ndizothekanso kulumikiza mbewa, kiyibodi, chowerengera barcode, chosindikizira cha DYMO kapena chosindikizira cha HP molunjika ku NanoPhotometer® Go.
Zindikirani: Tikupangira kugwiritsa ntchito ma drive a FAT/FAT32 opangidwa ndi 2.0 USB flash. Kukula kwa USB flash drive ndi chifukwa cha kusanjika kokhazikika komwe kuli 32 GB. Ma USB flash drive obisika samayenderana ndi NanoPhotometer® Go. Chidziwitso: Mbewa za Bluetooth zopanda chingwe sizimathandizidwa. Gwiritsani ntchito mbewa zamawaya zokha. Chidziwitso: Lumikizani mbewa ndi kiyibodi musanayambe NanoPhotometer® Go.
USB B
Pali doko la USB B lomwe lili kumbuyo kwa chida chomwe chimagwirizana ndi chingwe cha USB choperekedwa kuti mulumikize NanoPhotometer® Pitani pakompyuta. Kulumikizana kwa USB uku kungagwiritsidwe ntchito kuwongolera NanoPhotometer® Go kudzera pakompyuta.
LAN
Pali cholumikizira cha Ethernet (LAN) chakumbuyo kwa chida chomwe chimathandiza NanoPhotometer® Go kulumikizana ndi netiweki yakomweko. Kulumikizana kwa Efaneti kumeneku kungagwiritsidwe ntchito kusamutsa deta kuchokera ku NanoPhotometer® Pitani ku netiweki yapafupi, kuti muwongolere NanoPhotometer® Pitani kudzera pa chipangizo chowongolera ndi kusindikiza pamanetiweki. Kusamutsa deta kumatheka posunga pa netiweki chikwatu (onani tsamba 95 Network Foda) kapena pa NanoPhotometer® Go. file seva (onani tsamba 38 Kusamutsa Data kudzera File Seva).

NanoPhotometer® N50-Go/C40-Go User Manual Version 4.6.2 Dziwani: Pulagini chingwe cha LAN musanayambe NanoPhotometer® Go. Chidziwitso: Kutalika kwa chingwe cha LAN ndi 10 metres. Mtengo wocheperako ndi 1 Gbit / s
WiFi NanoPhotometer® Go ili ndi WiFi, yomwe imatha kugwiritsidwa ntchito ngati netiweki ya WiFi kapena WiFi Hotspot. Netiweki ya WiFi imalola magwiridwe antchito ofanana ndi kulumikizana kwa Ethernet kuphatikiza kusindikiza mwachindunji kudzera pa osindikiza a AirPrint / IPP omwe amathandizira mtundu wa PDF. Zindikirani: IPP version 2.2 ndiyofunika ndipo makonda ena osindikizira angafunikire kusinthidwa kuti athe kulumikizana ndi NanoPhotometer® Go. WiFi Hotspot imapereka mwayi wowongolera NanoPhotometer® Go ndi zida zina za WiFi monga makompyuta, mapiritsi kapena mafoni. Tsatanetsatane wa kulumikizana kwa WiFi Hotspot: SSID: NanoPhotometer® Go serial number Password: Implenuser Zindikirani: Chifukwa cha malire a zida zina zam'manja zosunga pazida zopanda zingwe zimangofikira miyeso 40 pa dataset iliyonse. Zosungira zazikulu zitha kusungidwa pa NanoPhotometer® Go yokha.
HDMI Pali doko la HDMI lomwe lili pagawo lakumbuyo la NanoPhotometer® Go lomwe limagwirizana ndi zingwe za HDMI 1.4 (kapena kuposa) kuti mulumikize NanoPhotometer® Pitani ku zowunikira zogwirizana ndi HDMI. Chidziwitso: Kutalika kwa chingwe cha HDMI ndi 5 mita.
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NanoPhotometer® N50-Go/C40-Go User Manual Version 4.6.2
NANOPHOTOMETER® GO MFUNDO

NanoVolume Performance N50-Go

Detection Range dsDNA Detection Range BSA Sample Volume Photometric Range (10 mm yofanana) Njira ya Dilution Factor kutalika

5 7,500 ng/µl 0.15 217 mg/ml 0.3 2 µl 0.1 150 A
0.67 ndi 0.07 mm 15 ndi 140

Cuvette Performance C40-Go

Kuzindikira Range dsDNA

0.1 - 130 ng/µl

Detection Range BSA

0.003 - 3.7 mg / ml

Mtundu wa Photometric

0 - 2.6 A

Kutalika Kwapakati (Z-Height)

8.5 mm

Mitundu ya Maselo

Kunja kwa 12.5 x 12.5 mm

Kutentha

37°C ± 0.5°C

Mawonekedwe Owonekera Wavelength Scan Range Yezerani Nthawi Yokwanira Sikaniyo Yamtundu Wavelength Reproducibility Wavelength Kulondola Bandwidth Absorbance Reproducibility
Kulondola kwa Absorbance Stray Light Optical Arrangement

C40-Pitani: 200 900 nm N50-Pitani: 200 650 nm 2.5 4.0 masekondi
C40-Pitani: ± 0.2 nm N50-Pitani: ± 1nm C40-Pitani: ± 0.75 nm N50-Go: ± 1.5 nm C40-Go: <1.5 nm N50-Go: <3 nm C40-Go (cuvette): <0.002 A @ 0 – 0.3 A @ 280 nm
CV <1% @ 0.3 2.0 A @ 280 nm N50-Go (Lid 15): <0.004 A (0.67 mm njira) @ 280 nm
CV <1% @ 0,3 – 1,5 A @ 280 nm <1.75 % @ 0.7 A @ 280 nm ya kuwerenga C40-Go: <0.5% @ 240 nm pogwiritsa ntchito NaI N50-Go: <2% @ 240 nm pogwiritsa ntchito NaI 1 x 4096 CMOS Array

Lamp Moyo wonse

Xenon kung'anima lamp Kuwala kwa 109, mpaka zaka 10

NanoPhotometer® N50-Go/C40-Go User Manual Version 4.6.2

Processing Mphamvu ndi Kugwirizana

Opareting'i sisitimu

Linux based OS

Purosesa ya Onboard

Intel Celeron wapawiri core 2.4 GHz

Kusungirako Data Kwamkati

C40-Go, N50-Go: 32 GB

Kugwirizana kwa Mapulogalamu

Windows 8, 10 (32 & 64 bit), OS X (Intel x86 ndi Apple M1), iOS ndi Android OS

Mfundo Zazikulu Kukula kwa Thupi Logwira Ntchito Voltage Display Certification In- ndi Output Ports Security

200 mm x 200 mm x 120 mamilimita 3.8 5.2 makilogalamu malinga ndi kasinthidwe 90 250 V ± 10%, 50/60 Hz, 90 W, 18/19 VDC 1024 x 600 mapikiselo; touchscreen magolovesi yogwirizana ndi CE, IEC 61010-1:2012 ndi EN 61326-1:2013 2x USB A, USB B, HDMI, Efaneti, WiFi Slot kwa Kensington loko

Mawonekedwe ndi mafotokozedwe amatha kusintha popanda kuzindikira. US Patents 20080204755 ndi 20080106742 Windows ndi chizindikiro cha Microsoft. Mac OS & iOS ndi zizindikiro za Apple, Inc. Android OS ndi chizindikiro cha Google. Linux ndi chizindikiro cha Linus Torvalds.

NanoPhotometer® N50-Go/C40-Go User Manual Version 4.6.2
2. KUYAMBIRA
Kuyika kwa SPECTROPHOTOMETER
ZINTHU ZACHITETEZO
Musanayambe kukhazikitsa, chonde patulani nthawi yodziwiratu zolemba zochenjeza ndi zizindikiro pa chida chanu ndi tanthauzo lake. Izi ndikudziwitsani ngati pali ngozi kapena kusamala komwe kukufunika. Kugwiritsa ntchito molakwika kumatha kuvulaza munthu kapena kuwononga chidacho. Chidacho chiyenera kugwiritsidwa ntchito ndi anthu ophunzitsidwa bwino komanso odziwa zambiri. Chonde werengani buku lathunthu la ogwiritsa ntchito musanagwiritse ntchito.
Direct panopa Overvoltage catetory: Kalasi II Kutalika kwakukulu kogwira ntchito: <2000 m Digiri ya kutayira: 2
Osatsegula chida chifukwa izi zitha kuwonetsa wogwiritsa ntchito mphamvu yamagetsi, kuwala kwa UV, ndi ma fiber optics osalimba kapena kuwononga chidacho.
Osagwiritsa ntchito zingwe zamagetsi zowonongeka, zowonjezera, ndi zotumphukira zina ndi NanoPhotometer® Go yanu. Gwiritsani ntchito magetsi operekedwa ndi otchulidwa okha.
Osawonetsa NanoPhotometer® Pitani kumalo amphamvu amagetsi, magetsi, madzi, mankhwala kapena mtundu uliwonse wamadzimadzi ngati mvula yamkuntho kapena chinyezi.
Musaike chidacho pamoto, chifukwa chikhoza kutupa kapena kuphulika. Osasunga kapena kugwiritsa ntchito pafupi ndi gwero la kutentha kwamtundu uliwonse, makamaka kutentha kopitilira 60°C kapena pamalo ophulika.
Osasiya NanoPhotometer® Pitani pachifuwa chanu kapena pafupi ndi gawo lililonse la thupi lanu kuti mupewe kusapeza bwino kapena kuvulala chifukwa cha kutentha.
Osayika zinthu pamwamba pa NanoPhotometer® Go.
Biological sampLes akhoza kukhala kapena kukhala ndi kuthekera kopatsirana matenda opatsirana. Dziwani kuopsa kwaumoyo komwe kumaperekedwa ndi otereamples ndi kuvala zida zodzitetezera zoyenera. Gwirani sampmosamala kwambiri komanso molingana ndi zofunikira zoyendetsera ntchito ndi bungwe musanagwire ntchito ndi zida zomwe zingathe kupatsirana. Chidziwitso: Osataya ma biologicals aliwonseamples pa zida zida. Ngati watayira, thirani tizilombo potsatira ndondomeko za mu labotale yanu komanso malangizo oyeretsera a chipangizocho (onani tsamba 107 la Maintenance).
Chizindikiro cha chinthucho, kapena pazikalata zotsagana ndi chinthucho, chikuwonetsa kuti chipangizochi sichingachitidwe ngati zinyalala zapakhomo. M'malo mwake idzaperekedwa kumalo osonkhanitsira omwe akuyenera kuti azibwezeretsanso zida zamagetsi ndi zamagetsi. Kutaya zinyalala kuyenera kutsatiridwa motsatira malamulo a zachilengedwe a mderalo.
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NanoPhotometer® N50-Go/C40-Go User Manual Version 4.6.2
KUSUKULUTSA NDIPONSO POKHALA
Yang'anani zomwe zili m'phukusi potengera zolemba zotumizira. Ngati shortagzapezeka, dziwitsani sapulani yanu nthawi yomweyo. Yang'anani chidacho kuti muwone ngati zawonongeka paulendo. Ngati kuwonongeka kulikonse kwapezeka, dziwitsani sapulani yanu nthawi yomweyo. Onetsetsani kuti malo omwe mukufuna kukhazikitsa akugwirizana ndi momwe chilengedwe chimagwirira ntchito motetezeka: kugwiritsa ntchito m'nyumba kapena malo owuma. Chidziwitso: Osawonetsa NanoPhotometer® yanu Pitani pafupi ndi zakumwa, mankhwala, mvula, chinyezi kapena malo afumbi. kutentha kwa ntchito 10 - 40 ° C; Ngati kutentha kwa cuvette kumagwiritsidwa ntchito kusiyanasiyana ndi 10 - 27 ° C. Kutentha kosungirako ndi 0 – 40°C. Osasunga chida pansi kapena pamwamba pa kutentha uku. Ngati chidacho chikukhudzidwa ndi kutentha kwakukulu, zingakhale zofunikira kulola kuti chidacho chigwirizane. Zimitsani chidacho ndikuyatsanso mukangokhazikika (~ 2 - 3 hours). Chinyezi chachikulu (chosatsika) cha 80% mpaka 31°C kutsika motsatizana kufika pa 50% pa 40°C. Chidacho chiyenera kuikidwa pamtunda wokhazikika, womwe ungathe kuthandizira 4-5 kg. Onetsetsani kuti mpweya umayenda momasuka mozungulira chidacho. Tsimikizirani poyatsidwa kuti palibe zinthu zomwe zimachepetsa kufalikira kwa mpweya. Pewani kuwala kwadzuwa chifukwa kungapangitse mbali zina za chipangizocho kukhala bleach komanso kuwonongeka kwa mapulasitiki. Zipangizozi zikhazikike kotero kuti pakagwa mwadzidzidzi pulagi yayikulu ikhoza kupezeka ndikuchotsedwa mosavuta. Nyamulani chidacho nthawi zonse pogwira corpus yayikulu ya chidacho osati mwachitsanzo pa chiwonetsero chomwe mwasankha kapena NanoVolume pedestal. Zidazi ziyenera kulumikizidwa ndi mphamvu ndi 90W magetsi / chingwe choperekedwa ndi Implen. Potulutsa magetsi ayenera kukhala ndi kondakitala woteteza (dziko lapansi / pansi). Itha kugwiritsidwa ntchito pa 90-250 V ± 10%, 50-60 Hz dongosolo lamagetsi. Chonde werengani buku lathunthu musanagwiritse ntchito koyamba. Yatsani chidacho pogwiritsa ntchito batani lamphamvu chakumbuyo chakumbuyo chikalumikizidwa. Chidacho chiziyesa zodziwikiratu. Chonde funsani wogulitsa choyambirira nthawi yomweyo ngati ukadaulo kapena sampndi zovuta kuthana nazo. Zindikirani: Ngati chipangizochi chikugwiritsidwa ntchito m'njira yomwe sinafotokozedwe kapena m'malo otetezedwa kuti chitetezeke, chitetezo choperekedwa ndi zidacho chikhoza kuwonongeka ndipo chitsimikiziro cha chidacho chidzasokonekera.
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NanoPhotometer® N50-Go/C40-Go User Manual Version 4.6.2
KUSINTHA KWA SOFTWARE
NPOS YATHAVIEW
NPOS ndi makina opangira ma Linux opangidwira NanoPhotometer® Go.
NPOS imatha kusunga zidziwitso ku bukhu wamba kapena kukonzedwa kuti isungidwe kuzinthu zodziyimira pawokha malinga ndi file mtundu ndi/kapena chida.
NPOS imatha kusunga deta mu mtundu wa Implen IDS, PDF kapena mtundu wa Excel file.
Chidziwitso: PDF ndi Excel files sangathe kutsegulidwa pa NanoPhotometer® Go. Files iyenera kusamutsidwa ku kompyuta kapena chipangizo komwe Excel kapena owerenga PDF alipo. Chidziwitso: Chonde musalumikizane ndi chida pakompyuta mpaka pulogalamu ya NanoPhotometer® Go NPOS itayikidwa pakompyuta.
ZOFUNIKA NDI KUGWIRIZANA
Mawonekedwe a ogwiritsa ntchito a NPOS adapangidwa kuti zinthu zonse zitha kugwiritsidwa ntchito pogwiritsa ntchito chophimba. Ngati pulogalamuyo imayikidwa pakompyuta popanda touchscreen, mawonekedwe ogwiritsira ntchito amatha kugwiritsidwa ntchito pogwiritsa ntchito kiyibodi ndi mbewa. Musanayambe kukhazikitsa, onetsetsani kuti pulogalamu ya chipangizo chowongolera ikugwirizana.
Yogwirizana Control zipangizo
Kompyuta: PC: Windows 8 / Windows 10 (32 & 64 bit) Mac: macOS Catalina / Big Sur (Intel x86 und Apple M1) Mapiritsi (zofunika zochepa): iPad: iOS 13 Android (quad core 1.2 GHz ndi 1 GB RAM) : Mafoni a m'manja a Android 10 (zofunika zochepa): iPhone: iOS 13 Android (quad core 1.2 GHz ndi 1 GB RAM): Mtundu wa Android 10
Windows ndi chizindikiro cha Microsoft. Mac OS & iOS ndi zizindikiro za Apple. Android OS ndi chizindikiro cha Google. Linux ndi chizindikiro cha Linus Torvalds.
Zindikirani: Pali mawonekedwe awiri ogwiritsira ntchito mapulogalamu omwe alipo; ina ya touchscreen yomangidwa, kompyuta ndi mapiritsi ndi ina ya mafoni.
KUYEKA SOFTWARE PA COMPUTER
Pulogalamu ya NanoPhotometer® Go ikhoza kukhazikitsidwa pamakompyuta ogwirizana a Windows ndi Mac. Makina ogwiritsira ntchito osiyanasiyana ndi zida zamakompyuta zitha kupangitsa njira yokhazikitsira kukhala yosiyana ndi yomwe yafotokozedwa apa. Njirayi imaperekedwa ngati chitsogozo chokha; zingafunike kusintha kwa machitidwe ena.
Chidziwitso: Osalumikiza NanoPhotometer® Pitani ku PC/Mac musanayambe kukhazikitsa NPOS. Chidziwitso: Ngati pulogalamu yam'mbuyomu ya NPOS idayikidwa kale pakompyuta, chotsani chingwe cha USB ndikuchotsa pulogalamu ya NPOS musanayike pulogalamu yatsopanoyi.
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NanoPhotometer® N50-Go/C40-Go User Manual Version 4.6.2. Dziwani izi: Mawindo ndi Mac unsembe files ali pa Implen USB flash drive yomwe imaphatikizidwa ndi NanoPhotometer® Go panthawi yobereka. The files zilipo kuti mutsitse kwaulere nthawi iliyonse kumalo otsitsa a Implen webtsamba (www.implen.de/downloads).
Kuyika kwa NPOS kwa wogwiritsa ntchito m'modzi pa Windows kompyuta 1. Yang'anani mtundu woyikidwa wa NanoPhotometer® Go fimuweya
(Zokonda/Za) ndikusintha kukhala mtundu waposachedwa ngati kuli kofunikira, musanayambe kukhazikitsa/kusintha pulogalamu ya NPOS pa kompyuta yanu. 2. Yambani kukhazikitsa NPOS file ndipo tsatirani ndondomeko ya kukhazikitsa kwa munthu mmodzi yekha. Kuyika file imapezeka pa Implen USB flash drive yomwe imaphatikizidwa ndi NanoPhotometer® Go panthawi yobereka kapena ikhoza kutsitsidwa kuchokera ku Implen. webtsamba: www.implen.de/downloads. Ulamuliro wathunthu umafunikira pakuyika. Ngati mulibe mwayi wokwanira, kukhazikitsa kungalephereke. Ngati mukukayika funsani woyang'anira kompyuta yanu. 3. Kuti mukhazikitse ogwiritsa ntchito ambiri (ofunikira pakukhazikitsa Windows kokha) sankhani pagawo la License Agreement posankha "Zapamwamba" ndi pazokambirana zotsatirazi "Ikani kwa onse ogwiritsa ntchito makinawa"
4. Yambitsani pulogalamu ya NPOS ndikusankha kugwirizana komwe mukufuna. Kuti mulumikizane ndi chingwe cha USB, lumikizani NanoPhotometer® Pitani ku PC pogwiritsa ntchito chingwe cha USB chomwe mwapereka. Kuti mulumikizane kudzera pa WiFi hotspot, onetsetsani kuti pali kulumikizana kokhazikika kwa WiFi pakati pa PC ndi NanoPhotometer® Go WiFi HotSpot (SSID: serial number, password: Implenuser). Kuti mulumikizane ndi netiweki, polumikizani NanoPhotometer® Pitani ku netiweki yapafupi kudzera pa chingwe cha Efaneti kapena netiweki ya WiFi (onani tsamba 92 Network).
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NanoPhotometer® N50-Go/C40-Go User Manual Version 4.6.2
Zindikirani: Ngati pakompyuta yanu pali Avira, ndibwino kuti muzimitsa chitetezo cha msakatuli. Izi zitha kusokoneza NPOS yomwe ikuyenda pa kompyuta yanu.
KUYEKA APP YA NANOPHOTOMETER® GO PA TABLET KAPENA SMARTPHONE
NanoPhotometer® Go App ikhoza kukhazikitsidwa ngati pulogalamu pamapiritsi ndi mafoni a m'manja omwe ali ndi machitidwe ogwiritsira ntchito a Android ndi iOS (onani tsamba 21 Zofunikira ndi Kugwirizana). NanoPhotometer® Go App ikupezeka kuti mutsitse kwaulere mu sitolo yamapulogalamu (Apple Store ndi Google Play Store).
1. Koperani ndi kukhazikitsa NanoPhotometer® Go App kuchokera mu app store 2. Lumikizani piritsi kapena foni yamakono kudzera pa WiFi HotSpot (SSID: Nambala ya seri,
password: Implenuser) kapena netiweki ya WLAN kupita ku NanoPhotometer® Go. 3. Tsegulani NanoPhotometer® Go App ndikusankha mtundu wa kulumikizana:
4. Mukalumikizidwa NanoPhotometer® Go idzazindikira piritsi / foni yamakono ngati chipangizo chakutali ndipo miyeso ingayambitsidwe kuchokera pa piritsi kapena foni yamakono.
5. Zotsatira zidzawonetsedwa pa piritsi kapena foni yamakono pamene miyeso yatengedwa.
Zindikirani: Kuti muyike NanoPhotometer® Go App pa tabuleti kapena foni yam'manja, chipangizochi chiyenera kukhala ndi intaneti yokhazikika kuti mulowetse pulogalamu yotsitsa pulogalamuyo. Zindikirani: Mtundu wa pulogalamuyi ndi pulogalamu ya NanoPhotometer® Go iyenera kukhala yofanana. Mabaibulo osiyanasiyana angakhale opanda ntchito zonse.
ZOCHITIKA ZOYAMBA NDI CONFIGURATION WIZARD
Mukayamba Implen NPOS nthawi yoyamba yomwe wizard yosinthira ya Implen ikuwonetsedwa. Chonde vomerezani Mgwirizano wa License Yogwiritsa Ntchito Mapeto (EULA) ndikusankha dziko limene NanoPhotometer® Go idzagwiritsidwa ntchito ndikutsimikizira.
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NanoPhotometer® N50-Go/C40-Go User Manual Version 4.6.2
PRINTER INSTALLATION
Kwa osindikiza olumikizidwa kudzera pa USB: 1. Sinthani NanoPhotometer® Pitani / chophimba chakunyumba 2. Lumikizani chosindikizira cha DYMO/HP kudzera pa USB chingwe 3. Chosindikizira cha DYMO/HP chakonzeka kugwiritsidwa ntchito pakadutsa masekondi 30
Chidziwitso: Onetsetsani kuti chophimba chakunyumba chikuwonetsedwa mukalumikiza chosindikizira. Ngati kulumikizana kwa USB pakati pa chosindikizira ndi NanoPhotometer® Go kukhazikitsidwa pomwe njira ili yotseguka, chosindikiziracho chikhoza kulephera. Nthawi zonse bwererani ku sikirini yakunyumba musanalumikize chosindikizira. Zindikirani: Onani ngati chosindikizira amagwirizana (tsamba 15 HP Printer) Kwa osindikiza a netiweki:
1. Tsimikizirani kulumikizidwa kwa netiweki ya LAN kapena ya WiFi 2. Khazikitsani chosindikizira IP mu zokonda (tsamba 96 Network Printer) 3. Printer ikupezeka m'njira zosindikizira Chidziwitso: Chongani chosindikizira chomwe chimagwirizana (tsamba 15 HP Printer)
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NanoPhotometer® N50-Go/C40-Go User Manual Version 4.6.2
3. NANOPHOTOMETER® GO BASICS
NanoPhotometer® Go product line imapereka yankho la NanoVolume (N50-Go) ndi ma standard cuvette (C40-Go) application. Mapulogalamu a NanoVolume amayamba ndi osachepera sampmphamvu ya 0.3 l. Mapulogalamu amtundu wa cuvette amatha kuchitidwa ndi 10 mm, 5 mm, 2 mm, 1 mm ndi 0.5 mm kutalika kwa quartz, galasi, kapena ma cuvettes apulasitiki okhala ndi kutalika kwa 8.5 mm.
MAFUNSO ATHAVIEW
NanoPhotometer® N50-Go imabwera ndi mapulogalamu awiri okonzedweratu, Nucleic Acids ndi Protein UV. Kuti musankhe njira, dinani chizindikiro chofananira ndipo njirayo imatsegulidwa nthawi yomweyo.
Pali njira yosinthira ya N50-Go yomwe ilipo. N50-Go yokwezedwa ndi N50 Touch yokhala ndi mwayi wogwiritsa ntchito zonse zomwe zilipo. Chowonekera chakunyumba C40-Go:
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NanoPhotometer® N50-Go/C40-Go User Manual Version 4.6.2

Njira zotsatirazi zilipo zokwezera N50-Go ndi C40-Go. *sikupezeka kwa N50-Go

Zithunzi za Njira

Kufotokozera

Nucleic Acids

Kuyika, kuyera, ndi kuphatikiza utoto kwa DNA, RNA, Oligo, ndi ma nucleic acid ena

Mapuloteni a UV

Kutsimikiza kwa mapuloteni a UV pa 280 nm (kapena mumitundu yosiyanasiyana ya 200 330 nm), chiyero ndi kuphatikiza utoto

Kinetics*

Nthawi vs. Kuwerenga kwa Absorbance

Mayeso a protein *

BCA (562 nm), Bradford (595 nm), Lowry (750 nm), ndi Biuret (546 nm) Mayeso

OD600*

Kuyeza kachulukidwe ka cell pa 600 nm (kapena osiyanasiyana 200 900 nm)

Kusungirako Zotsatira Zosungidwa za zotsatira zosungidwa

Njira Zosungidwa Zosungiramo njira zosungira zosungidwa

Mapulogalamu Ena*

Mapulogalamu owonjezera opezeka pa pulogalamu yachiwiri

Wavelength* Wavescan*

Tanthauzirani kutalika kwa mafunde amodzi kapena angapo pakati pa 200 900 nm (N50-Go: 200 650 nm) pakuyezera kuyamwa
Fotokozerani mtundu womwe mukufuna kusakatula kulikonse pakati pa 200 - 900 nm (N50-Go: 200 - 650 nm)

Kukhazikika*

Tanthauzirani coefficient ya kutha kwa mawerengedwe a ndende yokha

Absorbance/ Ration*

Tanthauzirani mawerengedwe a mafunde awiri a absorbance/rection

NanoPhotometer® N50-Go/C40-Go User Manual Version 4.6.2

Mzere Wopendekera* Pangani wokhotakhota wokhazikika pautali wodziwika

Mapulogalamu Amakonda *

Zosankha zomwe mwazokonda panjira zamunthu zomwe zimagwirizana ndi zosowa zamunthu payekha

Zithunzi

Dzina lachizindikiro

Zochita

WiFi Network WiFi network yogwira; mawonekedwe a kulumikizana kwa WiFi

WiFi Hotspot WiFi hotspot ikugwira ntchito

Thandizo

Imatsegula tsamba lothandizira

Zokonda Zimatsegula tsamba lokonda

Njira Yogulitsira Panyumba Yanyumba Sungani Deta

Imabwereranso ku sikirini yakunyumba yokhala ndi zithunzi za pulogalamu kuti musankhe njira.
Imatsegula dialog tumphuka ndi kuthekera kosunga njira yeniyeni yotsatirira ku njira yokhazikika
Imatsegula bokosi losunga mawu

Siyani Njira Yobwerera ku zomwe zasankhidwa kale

Kubwerera

Kubwerera kutsamba lapitalo (mafoni anzeru okha)

Chotsatira/Tsimikizirani Chimatsimikizira gawo ndikutsegula chinsalu chotsatira (smartphone yokha)

Sindikizani Zambiri

Imatsegula chosindikizira chosindikizira (chongowonetsedwa pamene chosindikizira chilipo)

Chotsani Data Kutsegula chofufumitsa kukambirana tumphuka

Parameter

Itsegula zenera la parameter

Zotsatira

Itsegula zenera lazotsatira

Chithunzi

Itsegula zenera la zotsatira za graph

Table

Tsegulani/kuwonetsa zotsatira mumtundu wa tebulo

Onjezani Foda Sinthani Zochotsa

Imawonjezera foda yatsopano pamndandanda
Imatsegula zokambirana zokhala ndi zosankha zingapo kuphatikiza kufufuta, kutchulanso kapena kulowetsa zikwatu/files/data komanso kukopera kapena kusuntha zikwatu/files/data kumayendedwe ofotokozedwa
Imachotsa ntchito zowonjezera mu parameter; imachotsa mawindo olowera

Kuletsa Kwathunthu

Imabwezeretsa graph kukhala kukula koyambirira popanda makulitsidwe
Kubwerera ku zenera lapitalo/kutseka zenera popanda kukhazikitsa zosintha zilizonse

NanoPhotometer® N50-Go/C40-Go User Manual Version 4.6.2

MABUTANI

Mukatsegula njira ndikuyamba ngatiampmuyeso, muyeso wopanda kanthu umafunika. Kwa muyeso wopanda kanthu, mwina madzi kapena sample buffer itha kugwiritsidwa ntchito kupatsa NanoPhotometer® Go chilozera cha zomwe ziro iyenera kukhala. Ndibwino kuti mugwiritsenso ntchito njira yopanda kanthu ndikuyesa ngatiample kuonetsetsa kuti chithunzi chazithunzi zopanda kanthu ndi mzere wathyathyathya.
Kuyambitsa spectral scan ya sampndi kukankha sampndi batani. Deta idzasungidwa kwakanthawi mpaka njirayo itatuluka; panthawiyi wogwiritsa ntchito ayenera kufotokozera ngati samples ayenera kupulumutsidwa.
Auto sampbatani la le limayimitsidwa mwachisawawa. Mukayatsa sampmiyeso imayambika yokha pomwe mkono wa chivindikiro watsekedwa. Auto sample function ikupezeka pa sample osati miyeso yopanda kanthu.

ZOYENERA KUYESA

NJIRA
Side Tab Bar
Kumanzere kwa chinsalu choyezera pali tabu yoyima yomwe ili ndi ma tabu anayi kuphatikizapo: magawo, zotsatira, graph, ndi tebulo. Ma tabu osiyanasiyana amalola wogwiritsa ntchito kukonza zowonera. Ndizotheka kuwonetsa kapena kubisa madera osiyanasiyana pazenera. Chinsalu chofikira pakompyuta chikuwonetsa madera onse, pazithunzi zomangidwa mkati ndi mtundu wa piritsi lomwe tebulo limabisika.

Parameter
Zotsatira
Chithunzi
Table
Zindikirani: Palibe tabu yopezeka pamitundu ya smartphone. Parameter, zotsatira ndi zowonetsera ma graph zikuwonetsedwa pazenera lonse. Ma parameters amayenera kutsimikiziridwa ( ) kuti afike pazithunzi zoyezera. Ndizotheka kusinthana pakati pa zotsatira ndi dera la ma graph posinthira kumanzere ndi kumanja. Palibe malo atebulo opezeka mafoni.
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NanoPhotometer® N50-Go/C40-Go User Manual Version 4.6.2
Malo a parameter M'dera la parameter ndizotheka kufotokozera zofunikira zonse zoyezera komanso kuyatsa njira ya cuvette ndikuyambitsa kutentha kwa cuvette. Chophimba choyezera choyezera chikuwonetsa malo omwe ali otsegulidwa mwachisawawa. Malo a parameter amabisidwa akayamba opanda kanthu kapena sample muyeso pokankhira pa Chopanda Chopanda kapena Sampndi batani. Ndizothekanso kubisa malo a parameter pogogoda tabu ya parameter mu vertical side tab bar.
Dera lazotsatira Gawo lazotsatira likuwonetsa njira yeniyeni ya muyeso wa imvi wowunikiridwa patebulo kuphatikiza ndende, ma absorbances, ndi ma ratios ofunikira. Ndizothekanso kusintha mayunitsi owerengera omwe amawerengedwa m'dera lazotsatira ndi menyu yosankha.
Malo a tebulo Malo a tebulo amasonkhanitsa zotsatira za mavesi onseamples mu njira yogwira. Gawo loyamba la tebulo likuwonetsa bokosi la tiki. Kusankha sampLes ndi bokosi la tick ma graph amakutidwa m'dera la graph. Ndi bokosi la tick lamutu ndizotheka kusankha / kuchotsa ma s onseamples (chiwerengero chachikulu cha sampkusankha ndi 30). Gawo lachiwiri la tebulo likuwonetsa ngati muyeso wasungidwa ( ) kapena osasungidwa (malo opanda kanthu).
Ndi batani losintha sampdzina la single sample akhoza kusinthidwa.
1. Sankhani sample mu tebulo (osankhidwa sampadzawonetsedwa mu imvi)
2. Dinani pa Sinthani batani
3. Kusintha sampdzina le
4. Tsimikizirani ndi batani la "Tsimikizirani" Dziwani: Sizingatheke kusintha sampmayina a IDS otsegulidwa files.
Malo a graph Malo a graph akuwonetsa tchati chokhala ndi graph ya muyeso weniweni kapena mizere yosankhidwa patebulo (kusankha bokosi la tiki). Pansi kumanzere kwa gawo la graph pali chowonjezera chowonjezera. Ngati njira yokulirapo yayatsidwa ma graph amiyezoyo azikutidwa okha. Kuti musinthe ma grafu okulirapo gwiritsani ntchito mabokosi a tick omwe ali patebulo.
Zindikirani: Ndizotheka kungophimba mpaka ma graph 30 pa tchati. Ngati data yopitilira 30 yasankhidwa mudzawoneka uthenga womwe umati "Kupitilira 30 samples asankhidwa. 30 okha ndi omwe awonetsedwa pa graph. " Zindikirani: Batani lophimba silikupezeka pa NanoPhotometer® Go touch screen ndi mafoni a m'manja, pamapiritsi ndi makompyuta okha.
Ndizotheka kuwonera ndi kunja mbali iliyonse ya chithunzicho (x- ndi y-axis). Sinthani makulitsidwe pokankhira chizindikiro cha sikelo yonse ( ).
Zindikirani: Makulitsidwe apamwamba kwambiri ndi 20 nm pa x-axis ndi 0.01A pa y-axis.
Monga nthano njira ya sampDzina lomwe lili patebulolo limapakidwa utoto wofanana ndi graph yomwe ili pa tchati. Kukankhira pa graph kumatsegula pop-up yomwe ikuwonetsa sample name, wavelength ndi
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NanoPhotometer® N50-Go/C40-Go User Manual Version 4.6.2 kuyamwa kwa kutalika kosankhidwa. Ndizotheka kuwonetsa zotsatira za ma graph ena posintha sample name ndi njira yotsitsa.
DATA PROCESSING DIALOGS
PRINT
Kusankha chizindikiro chosindikizira ( ) kumatsegula zenera la zenera lazenera lomwe lili ndi zosankha zosiyanasiyana zosindikiza. Chizindikiro chosindikizira chimangowonetsedwa ngati chosindikizira chilipo. Lamulo losindikiza limatumizidwa makamaka kwa osindikiza a DYMO kapena HP ngati alumikizidwa mwachindunji ku NanoPhotometer® Go kudzera pa chingwe cha USB. Ngati palibe chosindikizira cha USB chomwe chilipo, lamulo losindikiza limatumizidwa ku chosindikizira cha netiweki, ngati chakonzedwa. Makina osindikizira a netiweki atha kukhazikitsidwa mwazokonda polowetsa chosindikizira IP (onani tsamba 96 Network Printer). Zonse ndi samples amasindikizidwa. Zindikirani: Ngati chosindikizira chilumikizidwa mwachindunji ku NanoPhotometer® kudzera pa USB, chosindikizirachi chizikhala chofunikira kwambiri ndipo chidzagwiritsidwa ntchito mwachisawawa posankha Sindikizani pa NanoPhotometer®. Kuti musindikize pogwiritsa ntchito chosindikizira pa netiweki, chonde chotsani chosindikizira cha USB cholumikizidwa.
Zindikirani: Chizindikiro chosindikizira chimangowonetsedwa, ngati chosindikizira chilipo. Chidziwitso: Ndizotheka kugwiritsa ntchito chosindikizira chimodzi panthawi imodzi. Osalumikiza osindikiza ambiri ku NanoPhotometer® Go. Zindikirani: Njira yosindikiza sipezeka mu mapulogalamu a smartphone.
Sindikizani Paokha Ngati ntchito yosindikiza yokha yayatsidwa, muyeso uliwonse udzasindikizidwa mutatha kuyeza. Makina osindikizira akupezeka pa chosindikizira cha DYMO, chosindikizira cha HP cholumikizidwa kudzera pa chingwe cha USB ndi chosindikizira cha netiweki.
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NanoPhotometer® N50-Go/C40-Go User Manual Version 4.6.2 Zindikirani: Kuyika kofikira pa kusindikiza kwadzidzidzi kuzimitsa. Ngati yayatsidwa munjira imodzi imayikidwa yosasinthika panjira zonse ndipo iyenera kuzimitsidwa ngati sizikufunika. Zindikirani: Makina osindikizira okha sapezeka kuti asindikizidwe kudzera pa chipangizo chowongolera (chosindikiza chapakompyuta chapafupi).
Cryo Label Print Kuti musindikize pa zilembo za cryo lumikizani chosindikizira cha DYMO (4XL kapena 450) ku NanoPhotometer® Pitani ndikuyika pepala la cryo (26 x 12.7 mm ndi 9.5 mm mozungulira / mawonekedwe). Zindikirani: Pepala lolemba la Cryo silikupezeka/logwirizana ndi chosindikizira cha DYMO Label 5XL ndi 550.
PULUMUTSA
Kusankha chizindikiro chosunga deta ( ) kumatsegula zenera lazenera lazenera lomwe lili ndi zosankha zingapo zosungira.
Mwachikhazikitso onse sampLes amasankhidwa mugawo loyamba la tebulo ndipo adzapulumutsidwa. N'zotheka kusankha samples populumutsa pogwiritsa ntchito tickboxes. Bokosi la tick lamutu limasankha / kusasankha ma s onseamples. Zindikirani: Mu pulogalamu ya smartphone nthawi zonse mumakhala miyeso yonse yosungidwa, palibe kusankha komwe kungatheke.
Sungani monga Mtundu Ndi Save monga Mtundu njira ndizotheka kufotokozera file lembani kuti musunge. File zosankha zamtundu zikuphatikizapo Excel, PDF ndi Implen Document Source (IDS). N'zotheka kupulumutsa zosiyana file mafomu nthawi imodzi. Chidziwitso: IDS files sangathe kupulumutsidwa pazida zowongolera. IDS files ikhoza kusungidwa mu NanoPhotometer® yosungirako, chikwatu chofotokozera za netiweki kapena pa USB flash drive. Sizotheka kusunga IDS files kuchokera ku data yotsegulidwa. Pazifukwa izi, bokosi la tick la IDS limakhala lotuwa. Chidziwitso: PDF ndi Excel files sangathe kutsegulidwa pa NanoPhotometer® Go. The files kufunika
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NanoPhotometer® N50-Go/C40-Go User Manual Version 4.6.2
kusamutsidwira ku kompyuta kapena chipangizo komwe Excel kapena PDF yowerengera ilipo. Zindikirani: Zambiri zomwe zasungidwa pa NanoPhotometer® Go zimasungidwa mkati mwa Micro SD khadi. Ndibwino kuti musunge deta nthawi zonse ku hard drive ya kompyuta kapena netiweki. Nthawi zina kuti Micro SD khadi ikuwonongeka kwa data sikungathetsedwe.
Tsatirani Document Source File
The Implen Document Source (IDS) file ndi yeniyeni file mtundu, womwe ungatsegulidwe kokha ndi pulogalamu ya NPOS. Ndi hardcopy file zomwe sizingasinthidwe. Izi file mtundu uli ndi zidziwitso zonse zoyezera kuphatikiza data, zotsatira, makonda, ndi magawo.
Chidziwitso: Opulumutsidwa files ali ndi zosankhidwa zokhaamppafupi ndi nthawi file wapulumutsidwa.
Excel File
Deta yoyezera imatha kusungidwa ngati Excel file. Izi file mtundu uli ndi zidziwitso zonse zoyezera kuphatikiza data, zotsatira, ndi magawo.
Chidziwitso: Opulumutsidwa files ali ndi zosankhidwa zokhaamppafupi ndi nthawi file wapulumutsidwa.
PDF File
Deta yoyezera imatha kusungidwa ngati PDF file. Izi file mtundu uli ndi zidziwitso zonse zoyezera kuphatikiza data, zotsatira, ndi magawo. Ndi zotheka kukonza mizati ya tebulo la ma PDF ndi zosindikiza pazokonda (tsamba 97 Lipoti Kusintha).
Chidziwitso: Opulumutsidwa files ali ndi zosankhidwa zokhaamppafupi ndi nthawi file wapulumutsidwa.
File Dzina Lowetsani dzina la file. Zilembo zololedwa ndi: A…Z a…z 0…9 , . - () @! = _ ~; [ ] {} `
Zindikirani: Chilembo chopanda kanthu sichiloledwa.
Kusungirako Kuwonetsa chikwatu cha foda kuti musankhe malo osungira. Zosankha zikuphatikizapo: NanoPhotometer®, USB flash drive (ngati ikugwirizana), Foda ya Network (ngati itatha) ndi Control Chipangizo. Ngati Control Chipangizo chasankhidwa deta idzasamutsidwa ku chipangizo chowongolera chomwe chikugwirizana ndi NanoPhotometer® Go.
Chidziwitso: Sizotheka kusunga IDS files ku chipangizo chowongolera ngati kompyuta (PC/Mac), mapiritsi kapena mafoni.
Sungani Pagalimoto Kuti mupewe kutayika kwa data, miyeso yonse imasungidwa ngati IDS file pamtima wamkati wa NanoPhotometer® Go. Makopi osungira awa atha kupezeka mufoda ya Autosave ya NanoPhotometer® (Zotsatira Zosungidwa/NanoPhotometer/Autosave) mpaka masiku khumi. Files ali ndi dzina loyambira Backup, dzina la njira, ndi nthawi/deti stamp. Pambuyo masiku khumi autosave files amasamutsidwa kupita ku chikwatu chosungira zakale. Foda yosungiramo zinthu zakale imatha kupezeka kudzera pa NanoPhotometer® Go file seva (onani
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NanoPhotometer® N50-Go/C40-Go User Manual Version 4.6.2
Tsamba 38 Kusamutsa Data kudzera File Seva). Zomwe zili mufoda yosungira zosungira sizichotsedwa zokha.

Zomwe zili mufoda ya Autosave Archive zitha kuchotsedwa kudzera pa batani la zochita Zotsatira:

mu Stored

FUTA
Kusankha chithunzi chochotsa ( ) kumatsegula zenera lazenera lazithunzi zonse. Zonse zomwe zasankhidwa (chongani) pagawo loyamba la tebulo zidzachotsedwa. Bokosi la tick lamutu limasankha / kusasankha ma s onseamples. Yambitsani kufufuta ndi batani lochotsa. Tsimikizirani kuchotsedwa mu uthenga wochenjeza wotsatirawu: “Kodi mukufuna kuchotsa zonse/zosankhidwa files?” sankhani kuletsa (x) ibwereranso pazithunzi zochotsa kapena kutsimikizira ndi kufufuta kuti mufufute zomwe mwasankha. Zindikirani: Ntchito yochotsa sikupezeka pamtundu wa mapulogalamu opangidwira mafoni.
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NanoPhotometer® N50-Go/C40-Go User Manual Version 4.6.2
NJIRA ZOGWIRITSA NTCHITO
Ndi chithunzi chomwe mumakonda ndizotheka munjira iliyonse kusungira zoikamo za parameter kuti mupeze mosavuta njira zomwe zafotokozedwa. Sankhani zokonda zomwe mukufuna ndikutsegula kukambirana Njira Yosungidwa pokankhira o chizindikiro chomwe mumakonda.
Lowetsani dzina la njira ndikusankha malo osungira mufoda. Zosankha zikuphatikizapo: NanoPhotometer®, USB flash drive (ngati yolumikizidwa) ndi Foda ya Network (ngati itatha). Dinani batani la sitolo kuti musunge njira. Njira Zosungidwa zitha kutsegulidwa pazenera lanyumba potsegula Njira Zosungidwa (onani tsamba 87 Njira Zosungidwa).
KUGWIRA NTCHITO
NanoPhotometer® Go product line imapereka yankho la NanoVolume (N50-Go) ndi ma standard cuvette (C40-Go) application. Mapulogalamu a NanoVolume amayamba ndi osachepera sampmphamvu ya 0.3 l. Mapulogalamu amtundu wa cuvette amatha kuchitidwa ndi 10 mm, 5 mm, 2 mm, 1 mm ndi 0.5 mm kutalika kwa quartz, galasi, kapena ma cuvettes apulasitiki okhala ndi kutalika kwa 8.5 mm.
NANOVOLUME MEASUREMENT BASICS (N50-GO)
1. Sankhani njira malinga ndi sample ndikuyika magawo a muyeso.
2. Onetsetsani kuti sampzenera pa pedestal ndi kalilole mu chivindikiro mkono ali woyera. 3. Kwezani chivindikiro mkono ndi pipette mlingo woyenera akusowekapo yankho pa
kuwala samppawindo lakumbuyo. Kuwala kumangozimitsa pomwe mkono wa chivindikiro watsitsidwa.
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NanoPhotometer® N50-Go/C40-Go User Manual Version 4.6.2
Chidziwitso: Osadzaza chitsime. Zindikirani: Kuunikira kofiira (LED) kocheperako kungasinthidwe mu Zokonda 4. Tsitsani mkono wa chivindikiro ndikuyamba kuyeza kopanda kanthu ndi batani lopanda kanthu. minofu yaulere. Gwiritsani ntchito madzi, 5% ethanol kapena isopropanol ngati pakufunika.
Zindikirani: Onetsetsani kuti nkhope yachitsulo (yozungulira zenera loyezera ndi galasi) ndi yoyera. Zindikirani: Musagwiritse ntchito zosungunulira zaukali monga ma asidi amphamvu kapena maziko kapena zosungunulira organic nthawi iliyonse (onani tsamba 38 Kugwirizana kwa Solvent (N50-Go) Ngati simukutsimikiza lemberani support@implen-go.com kuti mudziwe zambiri za reagent yanu/ zosungunulira 6. N'zotheka kulowa ngatiampdzina la aliyense sample pawindo lolowera "lowetsani sampdzina lake". Chidziwitso: Zilembo zololedwa ndi: A…Z a…z 0…9 , . - () @! = _ ~; [ ] {} ` zilembo zopanda kanthu 7. Kwezani chivindikiro mkono ndi pipette kuchuluka koyenera kwa sample solution pa zowunikira sampndi zenera. Mukamaliza kuyeza, kwezani dzanja la chivindikiro, yeretsani pamalopo ndikugwiritsanso ntchito sekondi yotsatiraample. Zindikirani: Kuyika kwa Parameter Volume 1 - 2 µl kumasintha kutalika kwa njira. Kuyika kwa magawo Voliyumu 0.3 µl kumangotengera kutalika kwa njira ya 0.07 mm pazokwera kwambiri (dsDNA> 420 ng/µl / BSA> 12.6 mg/ml). Chidziwitso: The sampzenera lomwe lili pa pedestal liyenera kukhala loyera ndipo chotsalira chotsalira pa chopukuta chilichonse chiyenera kuchotsedwa kuti chigwire bwino ntchito.
ZOCHITIKA ZOYESA CUVETTE (C40-GO)
NanoPhotometer® Go imagwirizana ndi ma cuvettes wamba okhala ndi kutalika kwapakati kwa 8.5 mm. Njira yowala imasonyezedwa ndi mivi iwiri yoyera.
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NanoPhotometer® N50-Go/C40-Go User Manual Version 4.6.2
1. Sankhani njira malinga ndi sample ndikuyika magawo a muyeso.
2. Onjezani yankho lopanda kanthu kwa cuvette ndikuonetsetsa kuti voliyumu yodzaza ndi yokwanira kuti njira yowala idutse mu yankho.
3. Ikani cuvette mu chotengera cell. 4. Yambitsani muyeso wopanda kanthu ndi batani lopanda kanthu. Akamaliza kuyeza
chotsani cuvette. 5. Onjezani msample ku cuvette ndikuwonetsetsa kuti sample volume ndi yokwanira kulola kuwala
kudutsa sample. 6. Ndizotheka kulowa ngatiampdzina la aliyense sample pawindo lolowera "lowetsani sample
dzina”. Chidziwitso: Zilembo zololedwa ndi: A…Z a…z 0…9 , . - () @! = _ ~; [ ] {} ` zilembo zopanda kanthu 7. Yambani ngatiampmuyeso ndi sampndi batani. Mukamaliza kuyeza, chotsani cuvette. 8. Ikaninso samples.
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NanoPhotometer® N50-Go/C40-Go User Manual Version 4.6.2
SAMPMALANGIZO OTHANDIZA
Njira za NanoVolume (N50-Go)
Aample zenera pa pedestal limawunikiridwa (N50-Go) ndi nyali yofiyira yotsika kuti ithandizire ndi zolondola.ampndi application. Nyali yofiyira imazimitsidwa pamene mkono wa chivindikiro watsekedwa. Ndizotheka kuletsa mawonekedwe owunikira pazokonda za pulogalamu ya NPOS.
Voliyumu yochepa yomwe ingagwiritsidwe ntchito pa NanoVolume sampLes ndi 0.3 µl (N50-Go dsDNA> 420 ng/µl ndi BSA> 12.6 mg/ml). Kuti mukhazikitse kutalika kwa njira yokhayokha, osachepera 1µl amafunikira.
Voliyumu yayikulu yomwe ingagwiritsidwe ntchito pa NanoVolume sampLes ndi 2.0 µl (N50-Go). The sample akhoza kubwezeretsedwa kwathunthu pambuyo muyeso ndi pipette ngati mukufuna.
Zindikirani: Kuwonongeka kochepa sikungapewedwe pamlingo wa maselo. Kuyeretsa bwino ndikofunikira kuti mutsimikizire miyeso yolondola. Nthawi zambiri lint youma -
kupukuta kwa labotale kwaulere ndikokwanira kuyeretsa sampndi pamwamba pa quartz. Pankhani yokhazikika kwambiri sampzochepa kapena mapuloteni ena, njira yoyeretsera yoyeretsera ndiyo kugwiritsa ntchito chopukuta pang'ono chopanda lint (ndi madzi kapena 70% EtOH malinga ndi s.ample type) kuyeretsa bwino sample pamwamba. Ndikofunikira kuti nkhope yolumikizana ndi chitsulo kuzungulira zenera loyezera ndi galasi ikhale yoyera. Njira za Cuvette (C40-Go)
Chogwirizira cha cuvette chimagwirizana ndi quartz yokhazikika ya 10 mm, magalasi ndi ma cuvettes apulasitiki okhala ndi kutalika kwa 8.5 mm.
Ndikothekanso kugwiritsa ntchito ma cuvettes okhala ndi kutalika kwa 5 mm, 2 mm, 1 mm kapena 0.5 mm, koma pangakhale adaputala yofunikira. Chonde funsani amene akukupatsirani cuvette kuti akupatseni adaputala yoyenera.
Voliyumu yochepa yoyezera yolondola imadalira mtundu wa cuvette womwe umagwiritsidwa ntchito; ndikofunikira kuti kuwala kumadutsa mu sample kuti muyeze zolondola. Pakati kutalika ndi 8.5 mm.
Chidziwitso: Chosungira cha cuvette sichichotsedwa. Osatsanulira njira yoyeretsera mu chotengera cha cuvette chifukwa zakumwa zochulukirapo zitha kulowa mu chida ndikuwononga.
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NanoPhotometer® N50-Go/C40-Go User Manual Version 4.6.2

SOLVENT COMPATIBILITY (N50-GO)
Zosungunulira zambiri zomwe zimagwiritsidwa ntchito m'ma laboratories a sayansi ya moyo zimagwirizana ndi NanoPhotometer® Go NanoVolume s.ample pamwamba. Zosungunulira zotsatirazi ndizogwirizana kuti zigwiritsidwe ntchito ndi mitundu ya NanoPhotometer® N50-Go pa kutentha kwachipinda:

Acetone (5%)

Methylene kloride

Acetonitrile

MOPS

Benzene

Phenol (1%)

Butanol

N-propanol

Tetrachloride ya kaboni

Toluene

Chloroform

Phosphate yokhala ndi mabafa

Ethanol

PBS (pH 4-10)

Etere

Citrate

HEPES

Borate

Hexane Isopropanol MES

Chloride mchere
Acids > pH 2 Maziko <pH 10

Methanol

Zindikirani: Ma asidi okhazikika kwambiri ndi zoyambira sizovomerezeka. Ndi bwino kuti

pukuta sample pamwamba ndi labotale yopanda lint pukuta nthawi yomweyo ikamaliza

muyeso uliwonse. Kuti mudziwe zambiri za ngakhale kusungunulira kwapadera sikunatchulidwe

pamwambapa, chonde lemberani gulu lothandizira la Implen (support@implen-go.com) kuti muwone

kugwilizana.

KUSINTHA KWA DATA KUPITA FILE SERVER
Zambiri zomwe zasungidwa pa NanoPhotometer® Go zitha kupezeka mosavuta ndikusamutsidwa ku kompyuta kudzera pa NanoPhotometer® Go. file seva. Zosankha zolumikizira ndi LAN/WLAN, chingwe cha USB kapena WiFi Hotspot. Ndizotheka kupanga akaunti za ogwiritsa ntchito achinsinsi otetezedwa file mwayi wa seva. Maakaunti a ogwiritsa ntchito file mwayi wa seva ukhoza kutsegulidwa mu Zokonda onani tsamba 94 File Kufikira kwa Seva.
File Kufikira kwa Seva kudzera pa LAN/WLAN
Za ku file mwayi wa seva kudzera pa LAN/WLAN ndikofunikira kuti kompyuta ndi NanoPhotometer® Go zilumikizidwe ku netiweki yomweyo ya LAN/WLAN. Kuti mulumikizane ndi NanoPhotometer® Pitani ku LAN/WLAN onani tsamba 92 Network.

Pakompyuta ya Windows tsegulani Windows Explorer ndikulowetsa nambala ya serial kapena NanoPhotometer® Go IP mu bar ya adilesi ya Windows Explorer (mwachitsanzo \M80798 kapena
\ Adilesi ya IP yoperekedwa).

NanoPhotometer® N50-Go/C40-Go User Manual Version 4.6.2 Zindikirani: Nambala ya seriyo ndi IP adilesi ya NanoPhotometer® Go ingapezeke mu pulogalamu ya NanoPhotometer® Go pansi pa Zokonda/General/About. Pakompyuta ya MAC tsegulani dialog ya "Connect to Server" mu "Pitani" menyu ya Mac OS X Finder ndikulowetsa nambala ya NanoPhotometer® Go kapena NanoPhotometer® Go IP adilesi mu gawo la adilesi ya seva kuti mulumikizane.
Chidziwitso: Nambala ya seri ndi adilesi ya IP ya NanoPhotometer® Go zitha kupezeka mu pulogalamu ya NanoPhotometer® Go pansi pa Zokonda/General/About.
File Kufikira kwa Seva kudzera pa chingwe cha USB Kwa file mwayi wa seva kudzera pa chingwe cha USB, polumikiza NanoPhotometer® Go ndi chingwe cha USB A/B ku kompyuta ndikutsegula njira ya Windows Explorer kapena Connect to Server ya Mac (onani file kupeza seva kudzera pa LAN/WLAN) ndikulowetsa \192.168.7.1 kuti mulumikizane.
File Kufikira kwa Seva kudzera pa WiFi Hotspot Kwa file mwayi wa seva kudzera pa WiFi Hotpot WiFi Hotspot iyenera kukhala yogwira ntchito pa NanoPhotometer® Go. Kuti muyambitse onani tsamba 93
Zokonda pa WLAN. Kompyutayo iyenera kulumikizidwa ku NanoPhotometer® Go WiFi Hotspot (SSID: NanoPhotometer® Go serial number; password: Implenuser). Tsegulani Windows Explorer kapena Connect to Server njira ya Mac (onani file kupeza seva kudzera pa LAN/WLAN) ndikulowetsa \192.168.8.1 kuti mulumikizane.
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NanoPhotometer® N50-Go/C40-Go User Manual Version 4.6.2
4. NANOPHOTOMETER® GO APPLICATIONS
NanoPhotometer® Go imabwera ndi mapulogalamu okonzedweratu. Kwa N50-Go ndi njira yokhayo ya Nucleic Acid ndi Protein UV yomwe ilipo. Pali njira yokwezera ya N50 yomwe ikupezeka kuti mugwiritse ntchito kwathunthu. Njira iliyonse yogwiritsira ntchito imatha kusankhidwa ndikudina chithunzicho kamodzi kapena kudina chizindikirocho (mapulogalamu apakompyuta).
NUCLEIC AIDS
NJIRA YOPHUNZITSIRAVIEW
Nucleic acids mu njira imatenga kuwala ndi nsonga ya ultraviolet dera la 260 nm. Kuti mudziwe kuchuluka kwa ma nucleic acid mu yankho, kuyamwa kwa wavelength 260 nm kumagwiritsidwa ntchito limodzi ndi lamulo la Beer-Lambert. Kuphatikiza pa kuwerengera kuchuluka kwa ma nucleic acid, kuyeza kwa kuyamwa kumathandizanso pakuyerekeza kuyera kwa ma nucleic acid powerengera 260/280 nm ndi 260/230 nm. Kuphatikiza apo, ndizotheka kudziwa kuchuluka kwa ma nucleic acid okhala ndi ma probe kuphatikiza utoto wa fulorosenti. Sample ControlTM imapereka zambiri zothandiza za sample conditions. Imazindikira thovu la mpweya, sampzonyansa, turbidity, zotsalira za lint ndi zomwe zingaipitsidwe. Ngati Sample ControlTM imazindikira kusakhazikika kulikonse chizindikiro chochenjeza chikuwonetsedwa pazotsatira / tebulo. Kukankhira pachithunzi chochenjeza kukuwonetsa zambiri za kusakhazikika.
KUYENZA PROTOCOL
1. Sankhani chizindikiro cha Nucleic Acids pa skrini yakunyumba.
2. Kusintha mtundu wa nucleic acid kanikizani pa dsDNA ndipo mndandanda wokhala ndi zosankha umatsegulidwa kumanja. Zosankha ndi: dsDNA, ssDNA, RNA miRNA, miRNA Sequence, Oligo, Oligo Sequence and Custom (onani Table 1 patsamba 43). Ndizotheka kuyika dzina la mwambo wa nucleic acid factor pazolemba.
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NanoPhotometer® N50-Go/C40-Go User Manual Version 4.6.2 3. Pogwiritsa ntchito NanoVolume (N50-Go):
Sankhani kuchuluka kwa sample kuti agwiritsidwe.
Chidziwitso: 1 - 2 µl (chosasinthika): kuyika kwautali wa njira; 0.3 µl imayesa kutalika kwa njira ya 0.07 mm (kwa sampLes with concentrations > 420 ng/µl dsDNA) Pakugwiritsa ntchito cuvette (C40-Go): Sankhani kutalika kwa njira kutengera cuvette yomwe yagwiritsidwa ntchito. Zosankha ndi: 0.5 mm, 1 mm, 2 mm, 5 mm ndi 10 mm
Ngati akufuna kutentha sample mpaka 37°C gwiritsani ntchito toggle switch kuti muyatse chotenthetsera chokhala ndi cell. Pamene chosungira cha cuvette chafika 37 ° C kusintha kwa mtundu kumasintha kukhala wobiriwira. Zindikirani: Zopezeka pazogwiritsa ntchito za cuvette (C40-Go). 4. Sankhani Mayunitsi omwe ndende iyenera kuwerengedwa. Zosankha ndi ng/µl (zosasintha), µg/µl ndi µg/ml. pmol/µl ngati ndondomeko ya nucleic acid ilowetsedwa kuti muwerengere nucleic acid factor.
5. Kuwongolera kumbuyo kumathandizidwa pa 320 nm mwachisawawa. Zosankha zosankhidwa ndi 320 nm, 340 nm kapena kutalika kulikonse kwa 220 350 nm. Kusintha kwakumbuyo kumatha kuzimitsidwa ndi toggle switch.
6. Air kuwira kuzindikira ndi wolumala ndi kusakhulupirika. Kuyatsa kumazindikira thovu la mpweya, zotsalira za lint ndi zoyipaampzinthu za sample. Chidziwitso: Zotsalira za Lint ndi zoyipa zoyipaampLe zikhalidwe zimazindikirika ngakhale kuzindikira kwa kuwira kwa mpweya kwazimitsidwa.
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NanoPhotometer® N50-Go/C40-Go User Manual Version 4.6.2 7. Pa utoto wolembedwa samples tick zolemba za utoto pamndandanda, zomwe ziyenera kugwiritsidwa ntchito pazotsatira
kuwerengera. Zindikirani: Ngati utoto wogwiritsidwa ntchito sukupezeka pamndandanda, chonde pitani pazokonda ndikuwonjezera utoto wanthawi zonse pamndandanda wa utoto. Pali njira yosinthira utoto yomwe imatha kuyatsa / kuyimitsidwa ndikusintha kosinthira. Zindikirani: Kukonza utoto kumapezeka kokha posankha utoto umodzi.
8. Njira yoyika / kuwerengera gawo la dilution la s manual dilutedamples.
9. Ikani ddH20 yopanda kanthu kapena buffer ku s zowunikiraample zenera pa pedestal ndi kutseka chivundikiro cha mkono kuti muyezedwe ndikusankha opanda kanthu kuti muyambitse kuwerenga. Chidziwitso: Kuwunikira kwa sample zenera akhoza kuzimitsa mu zokonda.
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10. Gwiritsani ntchito chopukutira cha labotale chopanda lint kuti muyeretse zonse ziwiriample zenera pa pedestal ndi galasi mu chivindikiro mkono musanagwiritse ntchito s lotsatiraample.
Zindikirani: Zingakhale zothandiza kugwiritsa ntchito mawuwa kachiwiri ndikuwerenga ngatiample kuonetsetsa kuti palibe kanthu koyenera.

11. Ikani msample ku samptsegulani zenera pa pedestal ndikusindikiza samplembani batani kuti muyambe kuyeza.

KUWERENGA
Nucleic Acid Concentration
Kuti mudziwe kuchuluka kwa ma nucleic acid mu yankho, kuyamwa kumayesedwa pamlingo wa 260 nm. Ntchito yofotokozera mgwirizano pakati pa kukhazikika ndi kuyamwa ndikusinthidwa kwa malamulo a Beer-Lambert. Kuchuluka kwa nucleic acid samples ikhoza kuwerengedwa ndi kapena popanda kuwongolera zakumbuyo kutengera ndi gawo lowongolera / loyimitsa lakumbuyo.

Popanda kukonza zakumbuyo:

C = A260 * Factornuc * Ndi kuwongolera kumbuyo: C = (A260 – ABKG) * Factornuc*

Kukhazikika mu ng/µl

A260 ABKG

Absorbance pa 260 nm (njira 10 mm) Kuchotsa pamafunde osankhidwa akumbuyo (njira 10 mm) Manual dilution factor

Factornuc Nucleic acid factor mu ng*cm/µl

Table 1. Nucleic acids extinction coefficients (nuc)

Lembani dsDNA ssDNA RNA miRNA Oligo miRNA Seq. Oligo Seq. Mwambo

Factornuc 50 ng*cm/µl 37 ng*cm/µl 40 ng*cm/µl 33 ng*cm/µl 33 ng*cm/µl yowerengedwa pogwiritsa ntchito mphamvu ya kutha kwa ma nucleotides omwe amalowetsedwa kuwerengeredwa kudzera pakutha kokwanira kwa gawo lolowera mkati chinthu chilichonse pakati pa 15 ndi 150 ng*cm/µl

NanoPhotometer® N50-Go/C40-Go User Manual Version 4.6.2
Dye-labeled Nucleic Acid Concentration Kwa ma nucleic acid opangidwa ndi utoto, kuchuluka kwa nucleic acid kumawerengedwa pogwiritsa ntchito mawonekedwe osinthidwa a Beer-Lambert equation. Pamawerengedwe awa, chidacho chimaganizira kuchuluka kwa utoto ndi chinthu china chowongolera utoto pa 260 nm (onani Gulu 2 patsamba 46). Kuchuluka kwa nucleic acid wopangidwa ndi utoto kumawerengedwa ndi kapena popanda kuwongolera / utoto motere:
Ndi maziko komanso ndi kukonza utoto:
C = [(A260 – ABKG) – (cfdye * (Amax, dye – ABKG))] * Factornuc*
Ndi maziko komanso opanda kuwongolera utoto: C = (A260 – ABKG) * Factornuc * Popanda maziko komanso ndi kukonza utoto: C = [A260 – (cfdye * Amax, dye)] * Factornuc * Popanda maziko komanso popanda kukonza utoto:
C = A260 * Factornuc*

Kukhazikika mu ng/µl

A260

Absorbance pa 260 nm (njira 10 mm)

ABKG Absorbance pamafunde osankhidwa akumbuyo (njira 10 mm)

Amax, utoto Mtengo wa Absorbance pa kuchuluka kwa utoto (njira 10 mm)

Factornuc Nucleic acid factor mu ng*cm/µl

cfdye

Kuwongolera kodalira utoto pa 260 nm

Manual dilution factor

Zindikirani: Kukonza utoto kumapezeka kokha posankha utoto umodzi.

Kukhazikika kwa Udayi
Kwa ma nucleic acid opangidwa ndi utoto, kuchuluka kwa utoto kumawerengedwa pogwiritsa ntchito mawonekedwe osinthidwa a Beer-Lambert equation. Paziwerengerozi, chidachi chimaganizira kuchuluka kwa utoto, kuchuluka kwa kutha kwa utoto (onani Gulu 2 patsamba 46). Kuphatikizika kwa utoto kumawerengedwa ndikuwongolera kapena popanda kuwongolera motere:

Ndi kukonza zakumbuyo:

(Amax, dye - dye *

ABKG) 10-6

Popanda kukonza zakumbuyo:

Amax, dye * mdye * 10-6

NanoPhotometer® N50-Go/C40-Go User Manual Version 4.6.2

C Amax, utoto ABKG mdye

Kukhazikika mu ng/µl Mtengo wa Absorbance pakuyamwa kwambiri kwa utoto (njira 10 mm)
Absorbance pa 320 nm (njira 10 mm) Molar extinction coefficient of dye mu M-1*cm-1
Manual dilution factor

Nthawi zambiri Kuphatikizidwa (FOI)
FOI ndi kuchuluka kwa zilembo kutengera kuphatikizidwa kwa utoto mu cholembedwa cha nucleic acid s.ample. Nthawi zambiri amawonetsedwa ngati kuchuluka kwa mamolekyu a utoto omwe amaphatikizidwa pa ma nucleotide 1000. FOI ikhoza kuwerengedwa kapena popanda kuwongolera / kuwongolera utoto motere:

Ndi kukonza zakumbuyo ndikuwongolera utoto:

FOI =

324.5 * (Amax, utoto – ABKG)

utoto * 10-6 * (A260 – ABKG – cfdye * (Amax, utoto – ABKG)) * Factornuc

Ndi kukonza zakumbuyo popanda kukonza utoto:

FOI

324.5 mdye * 10-6 *

* (Amax, utoto – ABKG) (A260 – ABKG) * Factornuc

Popanda kuwongolera zakumbuyo komanso kukonza utoto:

FOI

mdye *

324.5 * Amax, dye 10-6* (A260 – cfdye * Amax, dye)

* Factornuc

Popanda kuwongolera zakumbuyo komanso popanda kukonza utoto:

FOI

324.5 * Amax, dye mdye * 10-6 * A260 * Factornuc

FOI
A260 ABKG Amax, dye mdye
Factornuc cfdye

Frequency of Incorporation (umbayi pa maziko 1,000) Absorbance pa 260 nm (10 mm njira) Kusowa pa utali wosankhidwa wakumbuyo (njira 10 mm) Kutaya kwa utoto pakumweka kwambiri kwa utoto (njira 10 mm) Kutha kwa utoto mu M-1 *cm-1
Nucleic acid factor mu ng * cm / µl Chomwe chimadalira utoto pa 260 nm

NanoPhotometer® N50-Go/C40-Go User Manual Version 4.6.2

Table 2. Mitundu ya Utoto, Absorbance Max, Extinction coefficient, ndi zowongolera zotengera utoto

NanoPhotometer® Go Model
N50-Go/C40-Go N50-Go/C40-Go N50-Go/C40-Go N50-Go/C40-Go N50-Go/C40-Go N50-Go/C40-Go N50-Go/C40-Go C40 -Pitani C40-Go C40-Go N50-Go/C40-Go N50-Go/C40-Go C40-Go C40-Go N50-Go/C40-Go N50-Go/C40-Go N50-Go/C40-Go C40 -Pitani C40-Go C40-Go N50-Go/C40-Go

Mtundu wa Dye

Absorbance maximum of Dye (nm)

Molar ext. khofi.
dye mdye mu M-1*cm-1

Alexa Fluor 350

346

Alexa Fluor 488

495

Alexa Fluor 532

532

Alexa Fluor 546

554

Alexa Fluor 555

555

Alexa Fluor 568

578

Alexa Fluor 594

590

Alexa Fluor 647

650

Alexa Fluor 660

663

Alexa Fluor 680

679

Cy3

550

Cy3.5

581

Cy5

649

Cy5.5

675

Oyster - 500

503

Oyster - 550

553

Oyster - 556

560

Oyster - 645

649

Oyster - 650

653

Oyster - 656

660

Texas Red

603

19,000 71,000 81,000 112,000 150,000 91,300 90,000 239,000 132,000 184,000 150,000 150,000 250,000 250,000 78,000 150,000 155,000 220,000 200,000 200,000 112,000

Chowongolera chodalira utoto pa 260 nm cfdye
0.25 0.30 0.24 0.21 0.08 0.45 0.43 0.00 0.00 0.00 0.08 0.08 0.05 0.05 0.29 0.05 0.03 0.05 0.04 0.04 0.23

Mawerengero
Zochita zogwiritsa ntchito ma nucleic acid nthawi zambiri zimafuna kuti pakhale chiyero chocheperako. Zowonongeka zamtundu wa nucleic acid sampLes zikuphatikizapo: mapuloteni, organic mankhwala, ndi zina. Kutengera zoipitsa wamba wa nucleic acid sampLes, ma ratios 260/280 ndi 260/230 amawerengedwa kuti ma nucleic acid apereke chizindikiritso cha chiyero cha s.amples. Kukonzekera koyera kwa DNA ndi RNA kumayembekezera 260/280 ma ratios a 1.8 ndi 2.0 motsatana. Kutsekemera kokwezeka kwa 230 nm kungasonyezenso kukhalapo kwa zonyansa; 230 nm ili pafupi ndi kuchuluka kwa zomangira za peptide ndipo ikuwonetsanso kuipitsidwa kwa bafa kuyambira TRIS, EDTA ndi mchere wina wotsekemera pa 230nm. Poyesa RNA sampzochepa, chiŵerengero cha 260/230 chiyenera kukhala> 2.0; chiŵerengero chotsika kuposa ichi nthawi zambiri chimasonyeza kuipitsidwa ndi guanidinium thiocyanate, reagent yomwe imagwiritsidwa ntchito kwambiri poyeretsa RNA ndipo imayamwa kupitirira 230-260 nm. Ngati chiŵerengero chapezeka kuchokera ku zovomerezeka
46

NanoPhotometer® N50-Go/C40-Go User Manual Version 4.6.2 sinthani chizindikiro chochenjeza chikuwonetsedwa pazotsatira/tebulo. Kukankhira pachizindikiro chochenjeza kukuwonetsa zambiri. Miyezo ya ziwerengero zovomerezeka zitha kufotokozedwa muzokonda. Ziwerengerozo zimawerengeredwa ndikuwongolera kapena popanda kuwongolera zakumbuyo kutengera ngati kuwongolera kwakumbuyo kumayendetsedwa panthawi yoyezera kapena ayi motere: Popanda kuwongolera kumbuyo: 260/280 ratio = A260
A280
260/230 chiŵerengero = A260
A230
Ndi kuwongolera kwakumbuyo: 260/280 chiŵerengero = A260 - ABKG
A280 - ABKG
260/230 chiŵerengero = A260 - ABKG
A230 - ABKG
47

NanoPhotometer® N50-Go/C40-Go User Manual Version 4.6.2
puloteni UV
NJIRA YOPHUNZITSIRAVIEW
Njira ya Protein UV imagwiritsa ntchito mayamwidwe achilengedwe a mapuloteni pa 280 nm kuphatikiza ndi Lamulo la Beer-Lambert, pomwe puloteni iliyonse imadziwika ndi puloteni yeniyeni ya extinction coefficient () yomwe ingagwiritsidwe ntchito kudziwa kuchuluka kwa mapuloteni a yankho. Kuyamwa kwamkati kwa mapuloteni kumachitika chifukwa cha kupezeka kwa ma amino acid onunkhira m'mapangidwe awo, makamaka tryptophan ndi tyrosine, komanso cysteine (oxidized cysteine residues mu disulphide bond). Zotsalira za amino acid onunkhira mu puloteni yomwe ili ndi tryptophan ndi tyrosine imawonetsa kuyamwa kwamphamvu kwapakati pa 280 nm, mothandizidwa ndi phenylalanine. Chifukwa chake, ndi zotsalira za amino acid zomwe zimapangitsa kuti puloteni iwonongeke pa 280 nm.
Njira yowongoka kwambiri yodziwira kuchuluka kwa puloteni yoyeretsedwa, yofanana ndi yomwe imadziwika kuti imatha kutha () ndikuyezera mwachindunji UV280 woperekedwa malinga ngati puloteni ilibe magulu opangira ma prosthetic omwe amayamwa mwamphamvu m'dera lomwelo. Komabe, kwa mapuloteni osadziwika kuphatikizapo mapuloteni osakanikirana, ndizotheka kupanga miyeso yolunjika ya A280 pogwiritsa ntchito mtengo wamagulu omwe amachokera kuyerekeza ndi mapuloteni ambiri, ngakhale kuti izi zidzangopereka pafupifupi koma kuyerekezera kwapafupi kwa mapuloteni enieni.
NanoPhotometer® Go imatsimikizira kuchuluka kwa mapuloteni pochita kuwerengera motengera zinthu zinazake, zomwe zidakonzedweratu mu chidacho kapena kulowetsedwa pamanja ndi wogwiritsa ntchito. Makhalidwe a Extinction coefficient () pa 280 nm amasiyana kwambiri ndi mapuloteni osiyanasiyana chifukwa cha kununkhira kwawo kwa amino acid. Miyezo yosasunthika imakonzedweratu mu pulogalamu yamapuloteni ena (onani Gulu 3 patsamba 52). Komabe, ngati puloteni yachiwongoladzanja sichikuphatikizidwa mu njira zokonzedweratu ndizothekanso kulowetsa pamanja puloteni yokondweretsa pogwiritsa ntchito mwambo wa Mol. Zowonjezera. Coefficient, Custom Ext. Coefficient kapena makonda 1/ protein factor. Kuti muwerenge molondola, m'pofunika kupereka izi: a) mphamvu ya molar extinction coefficient (M mu M-1*cm-1) ndi kulemera kwa molekyulu yowonetsedwa mu mayunitsi a molar (g/mol); b) kuchuluka kwa kutha (mu l/g*cm) kapena c) puloteni factor 1/ ya mapuloteni.
Kuti mudziwe kuchuluka kwa utoto wa mapuloteni, kuyamwa kumayesedwa pa kutalika kolingana ndi kuchuluka kwa utoto wa fluorescence kumagwiritsidwa ntchito (onani Gulu 3 patsamba 52). Chigawo chofananira cha kuzimiririka kwa utotowo chimagwiritsidwa ntchito limodzi ndi Lamulo la Beer-Lambert kudziwa kuchuluka kwa utoto.
Zindikirani: Ndikofunikira kuwonetsetsa kuti chiwongolero cha kutha ndi mayunitsi omwe adalowetsedwa ndi olondola kuti muwonetsetse kuti mawerengedwe akuchitidwa moyenera pamitengo yolondola.
Sample ControlTM imapereka chidziwitso chofunikira chokhudza thovu la mpweya, sampzonyansa, turbidity, zotsalira za lint ndi zomwe zingaipitsidwe. Ngati Sample ControlTM imazindikira kusakhazikika kulikonse chizindikiro chochenjeza chikuwonetsedwa pazotsatira / tebulo. Kukankhira pachithunzi chochenjeza kukuwonetsa zambiri za kusakhazikika.
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NanoPhotometer® N50-Go/C40-Go User Manual Version 4.6.2
KUYENZA PROTOCOL
1. Sankhani chizindikiro cha Protein UV pa zenera lanyumba.
2. Kusintha mtundu wa puloteni kanikizani pa BSA ndipo mndandanda wokhala ndi zosankha umatsegulidwa kumanja. Zosankha ndi: BSA, SA Mouse, SA Human, IgG Mouse, IgG Human, IgE Human, Lysozyme, OD1, Custom (Molar Extinction Coefficient), Custom (Extinction Coefficient) ndi Custom (1/). Kwa Mwambo (Mol. Ext. Coefficient) lowetsani Molecular Weight mu g/mol ndi Mol. Zowonjezera. Coefficient mu M-1*cm-1 For Custom (Ext. Coefficient) lowetsani Ext. Coefficient mu l/g*cm Pa Mwambo (1/) lowetsani chowerengera chowerengera mapuloteni 1/
3. Pa pulogalamu ya NanoVolume (N50-Go):
Sankhani kuchuluka kwa sample kuti agwiritsidwe.
Chidziwitso: 1-2 µl (chosasinthika): kusintha kwautali wa njira; 0.3 µl amangoyesa kutalika kwa njira ya 0.07 mm (kothekera kwa sampkuchepera ndi kuchulukira monga BSA> 12.6 mg/ml) Pakugwiritsa ntchito cuvette (C40-Go): Sankhani kutalika kwa njira kutengera cuvette yomwe yagwiritsidwa ntchito. Zosankha ndi: 0.5 mm, 1 mm, 2 mm, 5 mm ndi 10 mm
49

NanoPhotometer® N50-Go/C40-Go User Manual Version 4.6.2
Ngati akufuna kutentha sample mpaka 37°C gwiritsani ntchito toggle switch kuti muyatse chotenthetsera chokhala ndi cell. Pamene chosungira cha cuvette chafika 37 ° C kusintha kwa mtundu kumasintha kukhala wobiriwira. Zindikirani: Zopezeka pakugwiritsa ntchito kwa cuvette (C40-Go) 4. Kutalika kwa kutalika kwa miyeso ya mapuloteni kungasinthidwe mumtundu wa 200 330nm malingana ndi kutalika kwa kutalika kwa mapuloteni. Kukonzekera kosasintha ndi 280 nm 5. Sankhani Mayunitsi omwe ndende iyenera kuwerengedwa. Zosankha ndi ng/µl, µg/µl, µg/ml ndi mg/ml (zosasintha).
6. Kuwongolera kumbuyo kumathandizidwa pa 320 nm mwachisawawa. Zosankha zosankhidwa ndi 320 nm, 340 nm kapena kutalika kulikonse kwa 220 350 nm. Kusintha kwakumbuyo kumatha kuzimitsidwa ndi toggle switch.
7. Kuzindikira kuwira kwa mpweya kumayimitsidwa mwachisawawa. Pamene chinayambitsidwa detects mpweya thovu, lint zotsalira ndi osauka mikhalidwe ya sample. Chidziwitso: Zotsalira za Lint ndi zoyipa zoyipaampLe zikhalidwe zimazindikirika ngakhale kuzindikira kwa kuwira kwa mpweya kwazimitsidwa.
8. Kwa utoto wolembedwa samples, chongani zolemba za utoto pamndandanda womwe uyenera kugwiritsidwa ntchito powerengera zotsatira. Zindikirani: Ngati utoto wogwiritsidwa ntchito sukupezeka pamndandanda, chonde pitani pazokonda ndikuwonjezera utoto wanthawi zonse pamndandanda wa utoto. 50

NanoPhotometer® N50-Go/C40-Go User Manual Version 4.6.2 Pali njira yokonza utoto yomwe ingathe kuyatsidwa / kuyimitsidwa ndi kusintha kosintha.
Zindikirani: Kukonza utoto kumapezeka kokha posankha utoto umodzi.
9. Njira yoyika / kuwerengera gawo la dilution la s manual dilutedamples.
10. Ikani ddH20 yopanda kanthu kapena buffer ku s zowunikiraample zenera pa pedestal kuti muyezedwe ndikusankha opanda kanthu kuti muyambitse kuwerenga. Chidziwitso: Kuwunikira kwa sample zenera akhoza kuzimitsa mu zokonda.
11. Gwiritsani ntchito chopukutira cha labotale chopanda lint kuti muyeretse zonse ziwiriample zenera pa pedestal ndi galasi mu chivindikiro mkono musanagwiritse ntchito s lotsatiraample. Zindikirani: Zingakhale zothandiza kuyikanso kachiwiri ndikuwerenga ngatiample kuonetsetsa kuti palibe kanthu koyenera.
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NanoPhotometer® N50-Go/C40-Go User Manual Version 4.6.2

12. Ikani msample ku samptsegulani zenera pa pedestal ndikusindikiza samplembani batani kuti muyambe kuyeza.

KUWERENGA
Mapuloteni UV280 Concentration
Kuchuluka kwa mapuloteni mu njira ya Protein UV kumawerengedwa ndi kuchuluka kwa kuyamwa kwa sample pa 280 nm kapena kulowetsedwa kwa kutalika kwa 220-350 nm pamodzi ndi chiwonongeko chofotokozedwa ndi wogwiritsa ntchito. Kuchuluka kwa mapuloteni kumawerengedwa ndikuwongolera kapena popanda kuwongolera motere:

Ndi kuwongolera kumbuyo: C = (A280 - ABKG) * Factorprot * Ð

Popanda kukonza zakumbuyo:

C = A280 * Factorprot * Ð

Kukhazikika (mg/ml)

A280

Absorbance pa 280 nm (njira 10 mm) kapena kulowa mafunde

ABKG Absorbance pamafunde osankhidwa akumbuyo (njira 10 mm)

Factorprot Protein factor in g*cm/l (1/Ext. Coeff. or MW/Mol.Ext. Coeff.)

Dilution factor

Tebulo 3. Mapuloteni akusowa mphamvu (prot)

Type BSA SA Mouse SA Human IgG Mouse IgG Human IgE Human Lysozyme OD1

Factorprot [ g* cm/l] 1.499 1.493 1.718 0.714 0.735 0.654 0.379 1.000

Zowonjezera. Coeff. [l/g*cm] 0.6670 0.6700 0.5820 1.4000 1.3600 1.5300 2.6400 N/A

Mol. Zowonjezera. Coeff. [M-1*cm-1 ] 44,289 44,220 40,370 224,000 204,000 290,700 37,984 N/A

MW [g/mol] 66,400 66,000 69,365 160,000 150,000 190,000 14,388 N/A

Dye-labeled Protein UV280 Concentration
Kwa mapuloteni opangidwa ndi utoto, kuchuluka kwa mapuloteni kumawerengedwa pogwiritsa ntchito mawonekedwe osinthidwa a Beer-Lambert equation. Pazowerengera izi, chidacho chimaganizira kuchuluka kwa utoto, ndi chinthu china chowongolera utoto pa 280 nm (onani Gulu 4 patsamba 54). Kuphatikizika kwa utoto kumawerengedwa ndi kapena popanda kuwongolera / kuwongolera utoto motere:
Ndi maziko komanso ndi kukonza utoto:
C = [A280 – ABKG – (cfdye * (Amax, utoto – ABKG))] * Factorprot * Ð

NanoPhotometer® N50-Go/C40-Go User Manual Version 4.6.2

Ndi maziko komanso opanda kukonza utoto: C = (A280 – ABKG) * Factorprot * Ð Popanda maziko komanso ndi kukonza utoto:

C = (A280 – ( cfdye * Amax, utoto)) * Factorprot * Ð

Popanda maziko komanso popanda kukonza utoto:

C = A280 * Factorprot * Ð

C
A280 ABKG Amax, utoto Factorprot cfdye Ð

Kuyikira mu mg/ml Kutulutsa pa 280 nm (njira 10 mm) Kusakhazikika pamafunde osankhidwa akumbuyo (njira 10 mm) Kuchuluka kwa utoto pakuyamwa kwakukulu kwa utoto (njira 10 mm) Choyambitsa mapuloteni mu g*cm/l (1/Ext Coeff. kapena MW/Mol.Ext. Coeff.) Chowongolera chodalira utoto pa 280 nm Dilution factor

Kukhazikika kwa Udayi

Kwa mapuloteni olembedwa utoto, kuchuluka kwa utoto kumawerengedwa pogwiritsa ntchito mawonekedwe osinthidwa a equation ya Beer-Lambert. Paziwerengerozi, chidachi chimaganizira kuchuluka kwa utoto, komanso kutha kwamtundu wamtundu wamtundu wina (onani Gulu 4 patsamba 54). Kuphatikizika kwa utoto kumawerengedwa ndikuwongolera kapena popanda kuwongolera motere:

Ndi kukonza zakumbuyo:

((Amax, dye – ABKG) mdye * 10-6

*I)

Popanda kukonza zakumbuyo:

(Amax, dye * Ð) utoto* 10-6

C Amax, utoto ABKG mdye Ð

Concentration Absorbance pamtengo wokwanira wothira utoto (njira 10 mm)
Absorbance pa utali wosankhidwa wakumbuyo (njira 10 mm) Kutha kwa utoto mu M-1*cm-1
Dilution factor

Digiri ya zilembo (DOL)
DOL ndi kuchuluka kwa zilembo kutengera kuchuluka kwa mamolekyu a utoto ophatikizidwa ndi molekyulu ya protein. Kuchuluka kwa zilembo kumatha kuzindikirika kuchokera pamayamwidwe a antibody yomwe ili ndi kapena popanda kuwongolera / utoto motere:

NanoPhotometer® N50-Go/C40-Go User Manual Version 4.6.2

Ndi maziko komanso ndi kukonza utoto:

DOL =

(Amax, utoto - ABKG) * mprot

((A280 – ABKG) – cfdye * (Amax, dye – ABKG)) mdye

Ndi maziko ndi w/o kukonza utoto:

DOL =

(Amax, utoto (A280 -

- ABKG * mprot ABKG) * mdye

W/o maziko komanso ndi kukonza utoto:

DOL =

(A280

Amax, dye * mprot – cfdye * Amax, dye) * mdye

W/O maziko ndi w/o kukonza utoto:

DOL =

Amax, dye * mprot A280 * mdye

DOL
Amax, dye
A280 ABKG mdye mprot cfdye

Kuchuluka kwa zilembo/ utoto pa chiŵerengero cha mapuloteni a Absorbance pa kuchuluka kwa kuyamwa kwa utoto (njira 10 mm)
Absorbance at 280 nm (10 mm path) Absorbance at osankhidwa background wavelength (10 mm path) Extinction coefficient of dye in M-1*cm-1 Molar extinction coefficient of protein (M-1 * cm-1)
Kuwongolera kodalira utoto pa 280 nm

Table 4. Mitundu ya Utoto, Absorbance Max, Extinction coefficients, ndi zowongolera zotengera utoto

Mitundu ya NanoPhotometer® Go Dye Type

N50-Go/C40-Go N50-Go/C40-Go N50-Go/C40-Go N50-Go/C40-Go N50-Go/C40-Go N50-Go/C40-Go N50-Go/C40-Go N50 -Go/C40-Go C40-Go C40-Go C40-Go N50-Go/C40-Go C40-Go C40-Go

Alexa Fluor 350 Alexa Fluor 405 Alexa Fluor 488 Alexa Fluor 532 Alexa Fluor 546 Alexa Fluor 555 Alexa Fluor 568 Alexa Fluor 594 Alexa Fluor 647 Alexa Fluor 680 Alexa Fluor 790 Cy3 Cy5 DyLight 649

Absorbance maximum of Dye (nm)
346 401 495 532 554 555 578 590 650 679 785 550 649 654

Molar ext. khofi. wa Dye mdye mu M-1*cm-1
19,000 34,000 71,000 81,000 112,000 150,000 91,300 90,000 239,000 184,000 260,000 150,000 250,000 250,000

Chowongolera chodalira utoto pa 280 nm cfdye
0.19 0.70 0.11 0.09 0.12 0.08 0.46 0.56 0.03 0.05 0.08 0.05 0.05 0.04

NanoPhotometer® N50-Go/C40-Go User Manual Version 4.6.2

N50-Go/C40-Go

Chithunzi cha 488

493

70,000

0.15

N50-Go/C40-Go

Mtengo wa FITC

494

70,000

0.30

N50-Go/C40-Go

Pacific Blue

409

30,000

0.20

N50-Go/C40-Go

Pacific Orange

397

24,500

0.60

N50-Go/C40-Go

pHrodo Green

505

75,000

0.20

N50-Go/C40-Go

pHrodo Red

560

65,000

0.12

N50-Go/C40-Go

r-PE

566

1,863,000

0.17

N50-Go/C40-Go

Texas Red

595

80,000

0.18

Mawerengero
Mapuloteni sampmwachitsanzo kuchokera ku ma cell athunthu amatha kukhala ndi ma nucleic acid. Kuti muwone chiyero cha puloteni yokhayokha, chiŵerengero cha 260/280 chimawerengedwa kuti chipereke chizindikiro cha kuipitsidwa kwa nucleic acid. Kukonzekera kwa puloteni koyera kumakhala ndi ma 260/280 oyembekezeka a 0.57. Ngati chiŵerengero chazindikirika kuchokera pamlingo wovomerezeka chizindikiro cha chenjezo chikuwonetsedwa muzotsatira/mgawo la tebulo. Kukankhira pachithunzi chochenjeza kukuwonetsa zambiri. Miyezo ya ziwerengero zovomerezeka zovomerezeka zitha kufotokozedwa muzokonda. Chiyerekezocho chimawerengedwa ndi kuwongolera kapena popanda kuwongolera zakumbuyo kutengera ngati kuwongolera kwakumbuyo kumayendetsedwa panthawi yoyezera kapena ayi motere:
Popanda kukonza zakumbuyo:
260/280 chiŵerengero = A260
A280
Ndi kukonza zakumbuyo:
260/280 chiŵerengero = A260 - ABKG
A280 - ABKG

ZOYESA ZOPHUNZITSA
Ikupezeka pa C40-Go yokha, N50-Go ikufunika kukwezedwa kuti ma Protein Assays ayambitse.
NJIRA YOPHUNZITSIRAVIEW
Kuchuluka kwa mapuloteni kungayesedwe pogwiritsa ntchito zoyesa za colorimetric, momwe ma reagents ena amawonjezeredwa ku njira ya mapuloteni kuti apange mankhwala achikuda; mwina protein-cupric ion chelate monga mu Biuret, Lowry, BCA assays kapena mapuloteni-dye complex monga mu Bradford assay. M'mayeso a colorimetric awa, kuyamwa kumayesedwa mumtundu wowoneka pa utali woyenerera pa kuyesa kulikonse ndikufaniziridwa ndi mphira wokhazikika wokonzedwa ndi kusungunuka kwa serial kwa mulingo wa mapuloteni odziwika. Kusanthula kwa mzere, ziro kapena 2nd order regression regression of the calibration standard data point is taken by NanoPhotometer® Go. Coefficient coefficient (R2) mumtundu wa 0.95 mpaka 1.00 imasonyeza kukwanira bwino kwa mzere wowongoka.
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NanoPhotometer® N50-Go/C40-Go User Manual Version 4.6.2
Bradford Assay Method imadalira kuchuluka kwa kumangidwa kwa utoto, Coomassie Brilliant Blue, ku puloteni yosadziwika ndikuyerekeza kumangiriza kumeneku ndi kopindika kokhazikika kokonzedwa kuchokera ku gulu la mapuloteni odziwika omwe amadziwika pa 595 nm. Muyezo uwu nthawi zambiri umakhala BSA (bovine serum albumin).
Njira ya Biuret Assay imatengera momwe ma ion a cupric ndi ma peptide amachitira mu njira ya alkali, zomwe zimapangitsa kuti pakhale zovuta zoyamwa pa 546 nm.
BCA Assay Method imadalira momwe ma ions a cupric ions amachitira ndi ma peptide bond komanso kuzindikira kwa ma ion a cuprous pogwiritsa ntchito bicinchoninic acid (BCA), kupereka kuyamwa kwakukulu pa 562 nm. Njira ya BCA simakhudzidwa kwambiri ndi kupezeka kwa zotsukira zomwe zimagwiritsidwa ntchito kusungunula nembanemba.
Njira ya Lowry Assay imatengera zomwe Biuret amachita. Pansi pazikhalidwe za alkaline ion yamkuwa ya divalent imapanga zovuta ndi zomangira za peptide momwe zimachepetsedwa kukhala ion monovalent. Monovalent copper ion ndi magulu akuluakulu a tyrosine, tryptophan, ndi cysteine amachitira ndi Folin reagent kuti apange chinthu chosakhazikika chomwe chimachepetsedwa kukhala molybdenum/tungsten buluu. The bound reagent imasintha mtundu kuchokera kuchikasu kupita ku buluu. Kumangiriza kumeneku kumayerekezedwa ndi komwe kumapezeka ndi protein yokhazikika pa 750 nm; Izi nthawi zambiri zimakhala BSA (bovine serum albumin). Chidziwitso: Ma protocol atsatanetsatane amaperekedwa ndi zida zoyesererazi, ndipo amayenera kutsatiridwa kwambiri kuti zitsimikizire kuti zolondola zapezeka.
KUYENZA PROTOCOL
1. Sankhani chizindikiro cha zoyeserera zama protein kuchokera pazenera lakunyumba.
2. Kusintha mtundu woyeserera dinani Bradford ndipo mndandanda womwe uli ndi zosankha umatsegulidwa kumanja. Zosankha ndi: BCA Assay, Biuret Assay (osati ya N50 Go), Bradford Assay, Lowry Assay (osati ya N50-Go)
3. Kwa Cuvette Application (C40-Go): Sankhani kutalika kwa njira malinga ndi cuvette yogwiritsidwa ntchito. Zosankha ndi: 0.5 mm, 1 mm, 2 mm, 5 mm ndi 10 mm
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Ngati akufuna kutentha sample mpaka 37°C gwiritsani ntchito toggle switch kuti muyatse chotenthetsera chokhala ndi cell. Pamene chosungira cha cuvette chafika 37 ° C kusintha kwa mtundu kumasintha kukhala wobiriwira. Zindikirani: Zopezeka pa cuvette applicatons (C40-Go). Pa NanoVolume Application (yokha yokhala ndi N50-Go Upgrade): Sankhani dilution kutengera sampndi concentration
Zindikirani: Palibe zoikamo za kutalika kwa njira zomwe zilipo mwanjira iyi. Sankhani kuchepetsedwa kwa 15 (utali wanjira 0.67 mm) kapena 140 (utali wanjira 0.07 mm) kutengera sampndi concentration. 4. Miyezo yofikira pakuwongolera koyambira ikutengera mtundu wosankhidwa wa puloteni ndi mtundu wa chida: BCA mtengo wokhazikika 750 nm (N50: kuchotsedwa) Biuret kusakhazikika pamtengo wokhazikika wa Bradford 750 nm (N50: 350 nm) Mtengo wokhazikika wa 405 nm
Zindikirani: Ndikofunikira kugwiritsa ntchito zoyambira zoyambira pachiyeso chilichonse. 5. Sankhani mtundu wokwanira wa curve: Zosankha ndikubwerera kwa mzere, zero regression (kukakamiza mzere wowongoka
kupyolera mu chiyambi) ndi 2nd order regression.
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NanoPhotometer® N50-Go/C40-Go User Manual Version 4.6.2 6. Sankhani Unit
7. Onjezani mpaka 20 Concentrations pokankhira pa Add Concentration batani. Kuyika kowonjezera kumatha kuchotsedwa ndi Lowani milingo yokhazikika pamapindikira.
8. Sankhani Zosabwereza palibe, 2 kapena 3
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9. Yesani chopanda kanthu ndipo kutengera kusankha kobwereza, zonse zofunikira. Ma Absorbances of replicates adzawonetsedwa m'gawo lazotsatira ndipo ngati zobwereza zimasankhidwa mtengo wofunikira pamtundu uliwonse. Ndizotheka kusaphatikiza miyeso imodzi kuchokera pamawerengero a curve pozimitsa switch toggle. Zindikirani: Pambuyo pa sampmuyeso wokhotakhota wokhazikika sungathenso kusinthidwa. Njira yokhazikika ikapangidwa kapena kukwezedwa kuchokera ku njira yosungidwa idzagwiritsidwa ntchito powerengera ndende zoyezera s.amples. Zingakhale zofunikira kuchita muyeso wopanda kanthu.
10. Ikani msample ndikusindikiza sample batani kuyambitsa sampmuyeso. Zindikirani: Kamodzi sample muyeso wayambika sikuthekanso kusintha mayendedwe okhazikika.
KUSUNGA NDI KUKWEZA MAPIRITSI A STANDARD
Ndizotheka kusunga ma curve oyezedwa ngati Njira Yosungidwa. Kuti musunge kapindika wokhazikika kanikizani batani la njira yosungira ndikuyika dzina la njira, sankhani chikwatu ndikusunga ndi batani la Sungani. Njira zitha kutsegulidwa mumenyu ya Njira Zosungidwa patsamba loyambira. Kutsegula njira yosungidwa ya Protein Assay ikuwonetsa uthenga wokhala ndi mwayi wotsitsa kapena kuyezanso mayendedwe okhazikika.
KUWERENGA
Kuchuluka kwa mapuloteni kumatsimikiziridwa pogwiritsa ntchito mayendedwe okhazikika polumikizana ndi mayamwidwe a samples ndi ndende yodziwika kuwerengera kuchuluka kwa zosadziwika sample. Kuti mukhalebe olondola komanso olondola chonde onetsetsani kuti mtengo wa R2 wa curve wokhazikika ndi 0.95 kapena kupitilira apo.
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Zithunzi za KINETICS
Ikupezeka pa C40-Go yokha, N50-Go ikufunika kukwezedwa kuti Kinetics iyambe.
NJIRA YOPHUNZITSIRAVIEW
Ntchito ya Kinetics ndiyothandiza: kuyeza milingo yoyambira ya enzyme-catalyzed reaction, kupanga kusanthula kwamayendedwe athunthu, kuwerengera magawo oyambira a MichaelisMenten pamachitidwe a gawo limodzi, komanso kuyeza kuletsa kwa enzyme. Maphunziro osavuta a kinetics, pomwe kusintha kwa kuyamwa kumatsatiridwa ngati ntchito yanthawi pamlingo wokhazikika, kumatha kuchitidwa mosavuta ndi NanoPhotometer® Go. Mlingo wamankhwala amatha kuyeza pogwiritsa ntchito njira za spectrophotometric pophunzira kusintha kwa kuyamwa pamlingo wokhazikika ngati ntchito ya nthawi. Kusintha kwa kuyamwa uku kukuwonetsa kusintha komwe kumayenderana ndi ma reactants kapena zinthu zomwe zikuchitika. Mlingo wa mankhwala ambiri zimachitikira akhoza modziwika inapita patsogolo ndi kukhalapo kwa catalysts, amene amakhalabe mankhwala bwinobwino pa anachita. Zothandizira pazachilengedwe zimayimiriridwa ndi ma enzymes, omwe ndi othandizira mapuloteni apadera. Komabe, ena akaleampzinthu zina zapadera zoyambitsidwa ndi mamolekyu a RNA ziliponso. Kuwerenga ma kinetics of reaction kumatha kuwulula tsatanetsatane wofunikira wamakina othandizira omwe akukhudzidwa ndi masitepe otsatizana, kusintha kwa ma reactants kapena chikhalidwe cha ma enzyme inhibitors.

KUYENZA PROTOCOL

Chidziwitso: Ngati kinetic yayambika kuchokera pa chipangizo chowongolera kudzera pa intaneti ya WiFi (piritsi kapena foni yam'manja) ikani loko ya piritsi kapena foni yam'manja kuti isachitike. Apo ayi, kinetics idzasokonezedwa pamene foni yamakono kapena piritsi yatsekedwa, chifukwa cha kutaya kugwirizana kwa WiFi.
1. Sankhani chizindikiro cha Kinetics pa zenera lanyumba
2. Kwa Cuvette Application (C40-Go): Sankhani kutalika kwa njira malinga ndi cuvette yogwiritsidwa ntchito. Zosankha ndi: 0.5 mm, 1 mm, 2 mm, 5 mm ndi 10 mm
Ngati akufuna kutentha sample mpaka 37°C gwiritsani ntchito toggle switch kuti muyatse chotenthetsera chokhala ndi cell. Pamene chosungira cha cuvette chafika 37 ° C kusintha kwa mtundu kumasintha kukhala wobiriwira. Zindikirani: Zopezeka pazogwiritsa ntchito za cuvette (C40-Go).
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Pa NanoVolume Application (yokha yokhala ndi N50-Go Upgrade): Sankhani dilution kutengera sampndi concentration.
Zindikirani: Palibe zoikamo za kutalika kwa njira zomwe zilipo mwanjira iyi. Sankhani mwina kuchepetsedwa kwa 15 (utali wanjira 0.67 mm) kapena 140 (utali wanjira 0.07 mm). 3. Default wavelength () ndi 340 nm koma akhoza kusinthidwa mu osiyanasiyana 200 nm (N900: 50 nm), malingana ndi ntchito. 200. Zikhazikiko za nthawi: Lowetsani nthawi ya nthawi mu mphindi zomwe zikuyenera kuyesedwa. Zotheka
kutalika ndi 1 mphindi. Lowetsani nthawi yapakati pakati pa miyeso mumasekondi. Nthawi yofikira ndi 3000-
Masekondi 3,600 (N50: 10 sec.), kutengera nthawi yayitali. Lowetsani nthawi yochedwa mumasekondi musanayambe muyeso woyamba. Kuchedwa kotheka
nthawi ili pakati pa masekondi 0, kutengera nthawi yayitali.
Zindikirani: Kuchuluka kwa 500sampndi zotheka. Chonde ganizirani izi posankha nthawi ndi nthawi. 5. Ikani cuvette ndi zolozera sample ndikusankha batani lopanda kanthu kuti muyambe kuyeza. 6. Ikani cuvette ndi sample ndikusankha samplembani batani kuti muyambitse miyeso. Kinetic ikangoyambika batani Lopanda kanthu limatembenukira ku Imani / Pitirizani batani ndi Samplembani batani la Stop. Zindikirani: Pamene kinetics ikugwira ntchito sizingatheke kusintha magawo, kusunga deta kapena kuchotsa deta. Kusintha kwa magawo kumatheka musanayambe kuwerenga kwa kinetic. Sungani ndikuchotsa deta imapezeka pokhapokha gawo la kinetic layimitsidwa. Chidziwitso: Kusindikiza kwa auto ndi cryo label sikupezeka mu Kinetics.
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KUWERENGA
Makhalidwe onse a absorbance amasinthidwa kukhala njira ya 10 mm.

A0 Ndi dA Slope Final A
R2

= kuyamwa kwa mtengo woyambira (njira 10 mm)

= kuyamwa kwa nthawi yamtengo wapatali n (njira 10 mm)

= kuyamwa kwa mtengo weniweni wamtengo woyambira

= mzere wokhotakhota molingana ndi miyeso yonse yeniyeni

= kuyamwa kwa mtengo womaliza

ndi=1

(Oi-Ei)2 Ei

[Oyi

anaona

otsetsereka

mtengo;

kuyembekezera

otsetsereka

mtengo]

NanoPhotometer® N50-Go/C40-Go User Manual Version 4.6.2
Chithunzi cha OD600
Ikupezeka pa C40-Go yokha, N50-Go ikufunika kukwezedwa kuti OD600 iyambe.
NJIRA YOPHUNZITSIRAVIEW
Kukula kwa mabakiteriya muzinthu zamtundu wamadzimadzi nthawi zambiri kumayang'aniridwa poyesa kuchuluka kwa kuwala kwa 600 nm (OD600) m'ma s ang'onoang'ono.ampzotengedwa ku zikhalidwe. Miyezo ya OD600 imagwiritsidwa ntchito kudziwa stage wa kukula kwa bakiteriya chikhalidwe, potero kuonetsetsa kuti maselo kukololedwa pa akadakwanitsira mfundo lofanana ndi kachulukidwe yoyenera maselo amoyo. Kukula kwa maselo a bakiteriya kumapita patsogolo kudzera mu magawo angapo otsatizana monga: kusanja, chipika, kuyima ndi kutsika (onani Chithunzi 1 patsamba 64). Nthawi zambiri, ma cell amayenera kukololedwa chakumapeto kwa chipikacho pogwiritsa ntchito kuwala kwa samples kudziwa pamene mfundo imeneyi yafikiridwa. Popeza kachulukidwe ka kuwala pamiyeso ya OD600 imachokera ku kufalikira kwa kuwala m'malo moyamwa kuwala, mtengowu umasiyana malinga ndi mtundu wa maselo a bakiteriya mu chikhalidwe malinga ndi kukula ndi mawonekedwe. Maselo amakula nthawi zonse mpaka kuyamwa kwa 600 nm (kotchedwa OD 600) kufika pafupifupi 0.4 kusanachitike kapena kukolola. Ubale wa mzere ulipo pakati pa nambala ya cell (kachulukidwe) ndi OD 600 mpaka pamtengo woyamwa wa 0.6, pafupifupi.
Monga tafotokozera pamwambapa, kwa turbid sampmonga ma cell chikhalidwe, kuyamwa kuyeza kumachitika chifukwa cha kubalalika kwa kuwala, osati chifukwa cha kuyamwa kwa maselo. Popeza kuchuluka kwa kubalalikana kumakhudzidwa ndi mawonekedwe a dongosolo (kutalika pakati pa chosungira selo ndi chotulukapo chotuluka, monochromator optics, slit geometry, ndi zina), mitundu yosiyanasiyana ya ma spectrophotometer idzapereka mawerengedwe osiyanasiyana a OD 600 pamitundu yofanana ya turbid s.ample. Chifukwa chake, ngati zotsatira za ma spectrophotometer osiyanasiyana ziyenera kufananizidwa, ziyenera kusinthidwa kaye pogwiritsa ntchito ma curve oyenerera. Kuti mumve zambiri onani Technical Note #8 OD 600 yomwe imatha kutsitsidwa pa Implen webTsamba: www.implen-go.com/scientificpublications/
Njira yokhotakhota imatha kupangidwa poyerekeza kuyeza OD 600 ndi OD 600 yoyembekezeka. Kuyembekezeka kwa OD 600 kumatsimikiziridwa powerengera nambala ya cell pogwiritsa ntchito njira ina (yakale).ample microscope slide njira) ndi kutembenukira ku OD 600 pogwiritsa ntchito lamulo la chala kuti 1 OD 600 = 5 x 108 maselo/ml kwa E. coli.
NanoPhotometer® Go imabwera ndi chowongolera cha 1 mwachisawawa. Kuti mufananize milingo ya OD 600 pakati pa ma spectrophotometer osiyanasiyana, ndikofunikira kudziwa kupatuka kosalekeza kapena chiŵerengero pakati pa milingo ya kuyamwa kwa ma s ofanana.amplembani kuchokera pachida chilichonse ndikugwiritsira ntchito chinthuchi mkati mwa "correction factor" ya NanoPhotometer® Software yanu.
Chidziwitso: Kugwiritsa ntchito ma cuvettes otayidwa a 10 mm kutalika kwa njira kumalimbikitsidwa pakuyezera kachulukidwe kazinthu zama cell. Kuchuluka kwa ma cell kumawonekera pakuwerenga komanso kuthekera kwa kusinthasintha kwa maselo pakutsika kuchokera ku s.ample ku sample akhoza kuonedwa kuti ndi ofunika kwambiri. Chifukwa chake tikulimbikitsidwa kugwiritsa ntchito ma cuvettes popeza kuchuluka kwa zolakwika mu voliyumu yayikulu sikuli kofunikira. Miyezo ya cuvette imapereka avareji yokulirapo ndipo chifukwa chake mawerengedwe obwerezabwereza. Komanso, kupewa kuyimitsidwa kukhazikika mwachangu komanso kupereka kuwerenga kwa OD komwe kumasintha ndi nthawi, glycerol iyenera kuwonjezeredwa ku s.ample.
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Chithunzi 1 Bacterial kukula pamapindikira
KUYENZA PROTOCOL
1. Sankhani chizindikiro cha OD600 pa sikirini yakunyumba
2. Kwa Cuvette Application (C40-Go): Sankhani kutalika kwa njira malinga ndi cuvette yogwiritsidwa ntchito. Zosankha ndi: 0.5 mm, 1 mm, 2 mm, 5 mm ndi 10 mm
Ngati akufuna kutentha sample mpaka 37°C gwiritsani ntchito toggle switch kuti muyatse chotenthetsera chokhala ndi cell. Pamene chosungira cha cuvette chafika 37 ° C kusintha kwa mtundu kumasintha kukhala wobiriwira. Zindikirani: Zopezeka pazogwiritsa ntchito za cuvette (C40-Go). Pa NanoVolume Application (yokha yokhala ndi N50-Go Upgrade): Sankhani dilution kutengera sampndi concentration
Zindikirani: Palibe kuyika kwa kutalika kwa njira mwanjira iyi. Sankhani kuchepetsedwa kwa 15 (utali wanjira 0.67 mm) kapena 140 (utali wanjira 0.07 mm)
3. Mawonekedwe osasinthika ndi 600 nm koma kutalika kwake kungasinthidwe mumtundu wa 200 nm (N900-Go: 50 nm), malingana ndi ntchito.
4. Kusintha ma switch cell/ml kumayimitsidwa mwachisawawa. Thandizani ma cell/ml kuti ma cell/ml awerengedwe. Lowetsani ma cell enieni ndi kuchulutsa (monga 1 OD600 = 5 x 108 ma cell/ml) 64

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5. Lowetsani chinthu chowongolera kuti mulipirire masinthidwe osiyanasiyana a kuwala pakati pa NanoPhotometer® Go ndi zida zina.
6. Njira yosalala graph ndi mabokosi osiyanasiyana. Zosankha: Kuzimitsa, 1 = boxcar 11 (chosasinthika), 2 = boxcar 21 ndi 3 = boxcar 61
7. Njira yoyika / kuwerengera gawo la dilution la s manual dilutedamples.
8. Ikani cuvette ndi maumboni sample ndikusankha batani lopanda kanthu kuti muyambe kuyeza 65

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9. Ikani cuvette ndi sample ndikusankha samplembani batani kuti muyambe kuyeza.

KUWERENGA

OD600 = A600 * Ð * cf

Chithunzi cha OD600 A600

Kuchuluka kwa kuwala pa 600 nm Absorbance pa 600 nm (njira 10 mm) Dilution factor Correction factor for spectrophotometer

Maselo/ml = A600 * Ð * cf * multiplier

A600 Ð cf chochulukitsa

Absorbance pa 600 nm (njira 10 mm) Dilution factor Correction factor for spectrophotometer Multiplier of sample

NanoPhotometer® N50-Go/C40-Go User Manual Version 4.6.2
MA APPS ZAMBIRI
Ikupezeka pa C40-Go yokha, N50-Go ikufunika kukwezedwa kuti mutsegule Mapulogalamu Ambiri. Chizindikiro cha More Apps chomwe chili patsamba lakunyumba chimatsegula zenera lina la menyu ndi mwayi wopeza zithunzi za mapulogalamu owonjezera omwe amapezeka pa NanoPhotometer® Go. Mapulogalamu omwe ali mumndandandawu akuphatikiza: kutalika kwa mawonekedwe, kukhazikika, mafunde amafunde, kuyamwa / kuchuluka, mapindikidwe wamba ndi kugwiritsa ntchito mwamakonda.
ZINTHU ZAMBIRI: WAVELENGTH
Ikupezeka pa C40-Go yokha, N50-Go ikufunika kukwezedwa kuti mutsegule Wavelength.
NJIRA YOPHUNZITSIRAVIEW
Mu mawonekedwe a kutalika kwa mawonekedwe ndizotheka kuyeza kuyamwa kosavuta (A) ndi % transmittance (% Trans. / kokha mu cuvette mode) ngatiample pa mafunde enieni. Ndizotheka kuwonjezera mpaka 20 mafunde osiyanasiyana. Njira ya kutalika kwa mafunde imaphatikizapo chida chowerengera kuti chifotokoze ndikuwerengera ma formula omwe amafotokozedwa ndi kasitomala.
KUYENZA PROTOCOL
1. Sankhani chizindikiro cha More Apps kuchokera pa zenera lanyumba ndi chithunzi cha Wavelength kuchokera pa More Apps sikirini.
2. Pogwiritsa ntchito cuvette (C40-Go): Sankhani kutalika kwa njira malinga ndi cuvette yomwe yagwiritsidwa ntchito. Zosankha ndi: 0.5 mm, 1 mm, 2 mm, 5 mm ndi 10 mm
Ngati akufuna kutentha sample mpaka 37°C gwiritsani ntchito toggle switch kuti muyatse chotenthetsera chokhala ndi cell. Pamene chosungira cha cuvette chafika 37 ° C kusintha kwa mtundu kumasintha kukhala wobiriwira. Zindikirani: Zopezeka pazogwiritsa ntchito za cuvette (C40-Go). Pa ntchito ya NanoVolume (yokha ndi N50-Go Upgrade): Sankhani dilution kutengera sampndi concentration.
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Zindikirani: Palibe kuyika kwa kutalika kwa njira mwanjira iyi. Sankhani mwina kuchepetsedwa kwa 15 (utali wanjira 0.67 mm) kapena 140 (utali wanjira 0.07 mm). 3. Lowetsani kutalika komwe mukufuna () kuti muyesedwe. N'zotheka kuyeza mafunde 20 nthawi imodzi. Zosankha zambiri za kutalika () zitha kuwonjezeredwa posankha batani la Add Wavelength. Anawonjezera wavelength akhoza zichotsedwa ndi
4. Kuwongolera koyambira kumakhazikitsidwa mwachisawawa. Kuthandizira kuwongolera koyambira kukuwonetsa mndandanda wokhala ndi mitundu yosiyanasiyana ya kutalika kwa mafunde: 377 nm, 604 nm, 650 nm, 770 nm (N/A N50-Go) ndi 823 nm (N/A N50-Go). Njira yolowera kutalika kulikonse pakati pa 200 nm ndi 900 nm (N50-Go: 650 nm).
5. Njira yosalala graph ndi mabokosi osiyanasiyana. Zosankha: Kuzimitsa, 1 = boxcar 11 (chosasinthika), 2 = boxcar 21 ndi 3 = boxcar 61
6. Njira yolowetsa mafomu owerengera
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NanoPhotometer® N50-Go/C40-Go User Manual Version 4.6.2
Ndi zotheka kuyika mafomula 5 osiyanasiyana kuti muwerenge zotsatira. Malangizo Ovomerezeka a Fomula: 1. Nambala:
Kufikira ziwerengero zodziwika bwino za 20 ngati palibe cholekanitsa cha decimal chomwe chikugwiritsidwa ntchito Kufikira manambala ofunikira 4 ngati cholekanitsa cha decimal (nthawi ".") chagwiritsidwa ntchito 2. Ntchito Nambala: + (onjezani), - (chotsani), * (chulukitsani), / ( gawani) ndi mabatani () 3. Absorbance: Axxx mwachitsanzo pa kuyamwa pa 260 nm: A260 Dziwani: Musagwiritse ntchito zilembo zopanda kanthu. Eksample: Nucleic Acid (dsDNA) yowerengera yowerengera ndikuwongolera kumbuyo pa 320 nm: (A260-A320) * 50 7. Njira yoyika / kuwerengera dilution factor for manual diluted samples.
8. Ikani ddH20 yopanda kanthu kapena buffer ku s zowunikiraample zenera pa pedestal kuti muyezedwe ndikusankha opanda kanthu kuti muyambitse kuwerenga.
Chidziwitso: Kuwunikira kwa sample zenera likhoza kuzimitsidwa muzokonda.
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9. Gwiritsani ntchito chopukutira cha labotale chopanda lint kuti muyeretse zonse ziwiriample zenera pa pedestal ndi galasi mu chivindikiro mkono musanagwiritse ntchito s lotsatiraample. Zindikirani: Zingakhale zothandiza kuyikanso kachiwiri ndikuwerenga ngatiample kuonetsetsa kuti palibe kanthu koyenera.

Ikani sample ku samptsegulani zenera pa pedestal ndikusindikiza samplembani batani kuti muyambe kuyeza.

KUWERENGA
Kuwerengera kwa Fomula: Zimatengera fomula yomwe yalowetsedwa muzambiri za parameter.

Kuwerengera kwa Absorbance:
Absorbance imatanthauzidwa kuti ndi decimal logarithm (base 10) ya kubwereza kwa transmittance:

A = chipika (T1) = – chipika TT = 10(-A)

Zindikirani: Mtengo wofananira wa kuyamwa mwachitsanzo, mtengo wa Absorbance (= 230) etc. wokhazikika mpaka 10 mm kutalika kwa njira.

% Mawerengedwe a Transmittance (njira ya cuvette yokha)

Mu mawonekedwe a kutalika kwa mafunde ndizotheka kuyeza kuyamwa (A) ndi % transmittance (%T) ya asample ponena za kutchulidwa pa utali wina wa wavelength. Transmittance ndi chiŵerengero cha mphamvu ya kuwala yotsalira pambuyo podutsa mu sample (I) mpaka mphamvu ya kuwala koyambirira (I0):

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ZINTHU ZAMBIRI: WAVESCAN
Kupezeka kwa C40-Go kokha, N50-Go ikufunika kukwezedwa kuti Wavescan ayambitse.
NJIRA YOPHUNZITSIRAVIEW
Pogwiritsa ntchito mawonekedwe a wavescan ndizotheka kupeza mawonekedwe athunthu amtundu wa kutalika kwapakati pa 200-900 nm (C40-Go) kapena kuchokera ku 200-650 nm (N50Go/Kukweza kofunikira).
KUYENZA PROTOCOL
1. Sankhani chizindikiro cha More Apps kuchokera pa Home chophimba ndi chithunzi cha Wavescan kuchokera pa More Apps skrini
2. Pogwiritsa ntchito cuvette (C40-Go): Sankhani kutalika kwa njira malinga ndi cuvette yomwe yagwiritsidwa ntchito. Zosankha ndi: 0.5 mm, 1 mm, 2 mm, 5 mm ndi 10 mm
Ngati akufuna kutentha sample mpaka 37°C gwiritsani ntchito toggle switch kuti muyatse chotenthetsera chokhala ndi cell. Pamene chosungira cha cuvette chafika 37 ° C kusintha kwa mtundu kumasintha kukhala wobiriwira. Zindikirani: Zopezeka pazogwiritsa ntchito za cuvette (C40-Go). Pa ntchito ya NanoVolume (yokha ndi N50-Go Upgrade): Sankhani dilution kutengera sampLe concentration Zindikirani: Palibe kukhazikika kwa kutalika kwa njira mwanjira iyi. Sankhani kuchepetsedwa kwa 15 (utali wanjira 0.67 mm) kapena 140 (utali wanjira 0.07 mm)
3. Khazikitsani Start ndi End Wavelength kutanthauzira jambulani osiyanasiyana.
Zindikirani: Ngati sampLes yokhala ndi mawonekedwe amtundu wosiyana amasankhidwa, ma graph amawonetsedwa pazithunzi zonse za 200 nm (N900-Go: 50-200 nm). 650. Kuwongolera koyambira kumakhazikitsidwa mwachisawawa. Kuyatsa zokonza zoyambira zikuwonetsa mndandanda ndi
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NanoPhotometer® N50-Go/C40-Go User Manual Version 4.6.2 zosiyana za kutalika kwa kutalika: 377 nm, 604 nm, 650 nm, 770 nm (N/A N50) ndi 823 nm (N/A N50). Njira yolowera kutalika kulikonse pakati pa 200 nm ndi 900 nm (N50: 650 nm)
5. Njira yosalala graph ndi mabokosi osiyanasiyana. Zosankha: Kuzimitsa, 1 = boxcar 11 (chosasinthika), 2 = boxcar 21 ndi 3 = boxcar 61
6. Njira yoyika / kuwerengera gawo la dilution la s manual dilutedamples.
7. Ikani ddH20 yopanda kanthu kapena buffer ku s zowunikiraample zenera pa pedestal kuti muyezedwe ndikusankha opanda kanthu kuti muyambitse kuwerenga. Chidziwitso: Kuwunikira kwa sample zenera akhoza kuzimitsa mu zokonda.
8. Gwiritsani ntchito chopukutira cha labotale chopanda lint kuti muyeretse zonse ziwiriample zenera pa pedestal ndi galasi mu chivindikiro mkono musanagwiritse ntchito s lotsatiraample. Zindikirani: Zingakhale zothandiza kuyikanso kachiwiri ndikuwerenga ngatiample kuonetsetsa kuti palibe kanthu koyenera.
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batani kuyambitsa kuyeza.
KUWERENGA
Palibe kuwerengera kofunikira: ziwerengero zimanenedwa kutengera kutalika kwa 10 mm. Zotsatira zikuwonetsa nsonga zowoneka bwino zokhala ndi kutalika kwa mafunde ndi ma absorbance values. Pamiyezo ya cuvette ndizotheka kusintha kupita ku% Transmittance mode. Ngati chiŵerengero chapamwamba cha chidwi sichinasonyezedwe muzotsatira chiŵerengero chapamwambacho chingasankhidwe mwa kukankhira pa graph. Chiwongoladzanjacho chitha kuwonjezeredwa pazotsatira podina batani la Add Peak pawindo la pop-up.
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ZINTHU ZAMBIRI: ABSORBANCE RATIO
Ikupezeka pa C40-Go yokha, N50-Go ikufunika kukwezedwa kuti mutsegule Absorbance Ratio.
NJIRA YOPHUNZITSIRAVIEW
Munjira iyi, ndizotheka kudziwa masinthidwe osavuta a absorbance a sample poyesa kuyamwa pa mafunde awiri otchulidwa mu magawo a njira yokhudzana ndi chopanda kanthu.
KUYENZA PROTOCOL
1. Sankhani chizindikiro cha More Apps kuchokera pa sikirini yakunyumba ndi chizindikiro cha Absorbance/Ratio pa pulogalamu ya More
2. Pogwiritsa ntchito cuvette (C40-Go): Sankhani kutalika kwa njira malinga ndi cuvette yomwe yagwiritsidwa ntchito. Zosankha ndi: 0.5 mm, 1 mm, 2 mm, 5 mm ndi 10 mm
Ngati akufuna kutentha sample mpaka 37°C gwiritsani ntchito toggle switch kuti muyatse chotenthetsera chokhala ndi cell. Pamene chosungira cha cuvette chafika 37 ° C kusintha kwa mtundu kumasintha kukhala wobiriwira. Zindikirani: Zopezeka pazogwiritsa ntchito za cuvette (C40-Go). Pa ntchito ya NanoVolume (yokha ndi N50-Touch Upgrade): Sankhani dilution kutengera sampndi concentration
Zindikirani: Palibe kuyika kwa kutalika kwa njira mwanjira iyi. Sankhani mwina kuchepetsedwa kwa 15 (utali wanjira 0.67 mm) kapena 140 (utali wanjira 0.07 mm). 3. Lowetsani mafunde omwe mukufuna (1-1 ndi 1-2) kuti muwerengere chiŵerengero. Ndizotheka kuyeza mpaka 20 absorbance/retios nthawi imodzi. Mafunde ochulukirapo owerengera chiŵerengero amatha kuwonjezeredwa posankha batani la Add Ratio. Zowonjezera zowonjezera zitha kuchotsedwa ndi .
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NanoPhotometer® N50-Go/C40-Go User Manual Version 4.6.2 4. Kuwongolera koyambira kumayikidwa kukhala kosasintha. Kuyatsa zokonza zoyambira zikuwonetsa mndandanda
ndi mitundu yosiyanasiyana ya kutalika kwa mawonekedwe: 377 nm, 604 nm, 650 nm, 770 nm ndi 823 nm. Njira yolowera kutalika kulikonse pakati pa 200 nm ndi 900 nm (N50: 650 nm)
5. Njira yosalala graph ndi mabokosi osiyanasiyana. Zosankha: Kuzimitsa, 1 = boxcar 11 (chosasinthika), 2 = boxcar 21 ndi 3 = boxcar 61
6. Njira yoyika / kuwerengera gawo la dilution la s manual dilutedamples.
7. Ikani ddH20 yopanda kanthu kapena buffer ku s zowunikiraample zenera pa pedestal kuti muyezedwe ndikusankha opanda kanthu kuti muyambitse kuwerenga. Chidziwitso: Kuwunikira kwa sample zenera akhoza kuzimitsa mu zokonda.
8. Gwiritsani ntchito chopukutira cha labotale chopanda lint kuti muyeretse zonse ziwiriample zenera pa pedestal ndi galasi mu chivindikiro mkono musanagwiritse ntchito s lotsatiraample. Zindikirani: Zingakhale zothandiza kuyikanso kachiwiri ndikuwerenga ngatiample kuonetsetsa kuti palibe kanthu koyenera.
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9. Ikani msample ku samptsegulani zenera pa pedestal ndikusindikiza samplembani batani kuti muyambe kuyeza.

KUWERENGA

Chiŵerengero cha absorbance chimawerengedwa kuchokera ku njira ziwiri zomwe zimatchulidwa ndi wogwiritsa ntchito pazigawo.

1: 2

1 2

1: 2 = Absorbance Ratio 1 = Absorbance 1 yofanana ndi kuyamwa 1 yosankhidwa yokhazikika mpaka 10 mm njira
2 = Absorbance 2 yofananira kuyamwa 2 yosankhidwa yokhazikika mpaka 10 mm njira

NanoPhotometer® N50-Go/C40-Go User Manual Version 4.6.2
ZINTHU ZAMBIRI: CONCENTRATION
Ikupezeka kokha pa C40-Go, N50-Go ikufunika kukwezedwa kuti mutsegule Concentration.
NJIRA YOPHUNZITSIRAVIEW
Munjira iyi, ndende imatha kuwerengedwa ngatiample pozindikira kuyamwa pa utali wina wa wavelength wokhudzana ndi kalozera. ndende ndiye analandira ndi kuchulukitsa kuyeza absorbance ndi yeniyeni chinthu. Chinthuchi chikhoza kudziwidwa pasadakhale ndikulowetsedwa ndi wogwiritsa ntchito, kapena chikhoza kuwerengedwa ndi chidacho poyesa ndondomeko yokhazikika (njira yokhazikika yokhotakhota) ndi zodziwika zodziwika kuti apange mphira wokhazikika.
KUYENZA PROTOCOL
1. Sankhani chizindikiro cha More Apps kuchokera pa sikirini yakunyumba ndi chizindikiro cha Concentration kuchokera pa More Apps sikirini.
2. Pogwiritsa ntchito cuvette (C40-Go): Sankhani kutalika kwa njira malinga ndi cuvette yomwe yagwiritsidwa ntchito. Zosankha ndi: 0.5 mm, 1 mm, 2 mm, 5 mm ndi 10 mm
Ngati akufuna kutentha sample mpaka 37°C gwiritsani ntchito toggle switch kuti muyatse chotenthetsera chokhala ndi cell. Pamene chosungira cha cuvette chafika 37 ° C kusintha kwa mtundu kumasintha kukhala wobiriwira. Zindikirani: Zopezeka pazogwiritsa ntchito za cuvette (C40-Go). Pa ntchito ya NanoVolume (yokha ndi N50-Go Upgrade): Sankhani dilution kutengera sampndi concentration.
Zindikirani: Palibe kuyika kwa kutalika kwa njira mwanjira iyi. Sankhani mwina kuchepetsedwa kwa 15 (utali wanjira 0.67 mm) kapena 140 (utali wanjira 0.07 mm). 3. Default wavelength ndi 260 nm koma akhoza kusinthidwa mu osiyanasiyana 200900 nm (N50: 200 nm), malinga ndi sample/application. 4. Lowetsani chinthu chowerengera ndende.
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6. Kuwongolera koyambira kumakhazikitsidwa mwachisawawa. Kuthandizira kuwongolera koyambira kukuwonetsa mndandanda wokhala ndi mitundu yosiyanasiyana ya kutalika kwa mafunde: 377 nm, 604 nm, 650 nm, 770 nm (N/A N50) ndi 823 nm (N/A N50). Njira yolowera kutalika kulikonse pakati pa 200 nm ndi 900 nm (N50: 650 nm)
7. Njira yosalala graph ndi mabokosi osiyanasiyana. Zosankha: Kuzimitsa, 1 = boxcar 11 (chosasinthika), 2 = boxcar 21 ndi 3 = boxcar 61
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8. Njira yoyika / kuwerengera gawo la dilution la s manual dilutedamples.
9. Ikani ddH20 yopanda kanthu kapena buffer ku s zowunikiraample zenera pa pedestal kuti muyezedwe ndikusankha opanda kanthu kuti muyambitse kuwerenga. Chidziwitso: Kuwunikira kwa sample zenera akhoza kuzimitsa mu zokonda.
10. Gwiritsani ntchito chopukutira cha labotale chopanda lint kuti muyeretse zonse ziwiriample zenera pa pedestal ndi galasi mu chivindikiro mkono musanagwiritse ntchito s lotsatiraample. Zindikirani: Zingakhale zothandiza kuyikanso kachiwiri ndikuwerenga ngatiample kuonetsetsa kuti palibe kanthu koyenera.
11. Ikani msample ku samptsegulani zenera pa pedestal ndikusindikiza samplembani batani kuti muyambe kuyeza.
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KUWERENGA
Mwa njira iyi, ndende ya sample imawerengedwa kutengera lamulo la Beer-Lambert lomwe limaperekedwa ndi wogwiritsa ntchito molingana ndi kutalika kwa chiwongoladzanja komanso kuchuluka kwa kutha kwa wogwiritsa ntchito. Ma equations owerengera ndende popanda kuwongolera kumbuyo ndi awa:

Popanda kukonza zakumbuyo:

= Ndi

Kukhazikika (ng/µl)

Kusowa kwa wogwiritsa ntchito njira yayitali n (njira 10 mm)

Dilution factor

extinction coefficient/factor

NanoPhotometer® N50-Go/C40-Go User Manual Version 4.6.2
ZINTHU ZAMBIRI: STANDARD CURVE
Ikupezeka pa C40-Go yokha, N50-Go ikufunika kukwezedwa kuti mutsegule Standard Curve.
NJIRA YOPHUNZITSIRAVIEW
Kupanga kokhotakhota kowongolera kuchokera kumagulu angapo odziwika bwino kumatha kupangidwa ndikusungidwa pa NanoPhotometer® Go. Mzere wokhotakhota ungagwiritsidwe ntchito kuwerengera sampzochepa zamtundu womwewo wokhala ndi milingo yosadziwika. Pulogalamuyi imapereka chida chothandiza kwambiri chomwe mungaphatikizire, kufulumizitsa ndi kufewetsa muyeso ndi kuwerengera komwe kumakhudzidwa pozindikira kuchuluka kwa ma analytes m'magawo osadziwika.amples. Ngati ziro zowerengera zikufunika, ziphatikizireni pamiyezo yomwe ikuyenera kulowetsedwa pogwiritsa ntchito reagent yopanda kanthu ndikulowetsa 0.00 kuti muyike.
KUYENZA PROTOCOL
1. Sankhani chizindikiro cha More Apps kuchokera pa sikirini yakunyumba ndi chizindikiro cha Standard Curve kuchokera pa More Apps sikirini.
2. Pogwiritsa ntchito cuvette (C40-Go): Sankhani kutalika kwa njira malinga ndi cuvette yomwe yagwiritsidwa ntchito.
Ngati akufuna kutentha sample mpaka 37°C gwiritsani ntchito toggle switch kuti muyatse chotenthetsera chokhala ndi cell. Pamene chosungira cha cuvette chafika 37 ° C kusintha kwa mtundu kumasintha kukhala wobiriwira. Zindikirani: Zopezeka pamapulogalamu a cuvette (ndi C40-Go). 3. Pogwiritsa ntchito NanoVolume (pokhapokha ndi N50-Go kukweza): Sankhani dilution kutengera sampndi concentration
Zindikirani: Palibe kuyika kwa kutalika kwa njira mwanjira iyi. Sankhani mwina kuchepetsedwa kwa 15 (utali wanjira 0.67 mm) kapena 140 (utali wanjira 0.07 mm). 4. Kuwongolera koyambira kumakhazikitsidwa mwachisawawa. Kuthandizira kuwongolera koyambira kukuwonetsa mndandanda wokhala ndi mitundu yosiyanasiyana ya kutalika kwa mafunde: 377 nm, 604 nm, 650 nm, 770 nm (N/A N50) ndi 823 nm (N/A N50). Njira yolowera kutalika kulikonse pakati pa 200 nm ndi 900 nm (N50: 650 nm)
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mzere kupyolera mu chiyambi) ndi 2nd dongosolo regression. 6. Sankhani Unit
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NanoPhotometer® N50-Go/C40-Go User Manual Version 4.6.2 7. Onjezani mpaka 20 Concentrations pokankhira batani la Add Concentration. Zowonjezedwa
ndende akhoza zichotsedwa ndi Lowani ndende ya muyezo pamapindikira.
8. Sankhani Zosabwereza palibe, 2 kapena 3
9. Kamodzi kokhotakhota kokhazikika kupangidwa kapena kunyamulidwa kudzagwiritsidwa ntchito powerengera ndende munjira. Zingakhale zofunikira kuchita muyeso wopanda kanthu.
10. Ikani msample ndikusindikiza samplembani batani kuti muyambe kuyeza. Zindikirani: Kamodzi sample kuyeza kumayambika sikutheka kupanga masinthidwe kumayendedwe okhazikika.
KUSUNGA NDI KUKWEZA MAPIRITSI A STANDARD
Ndizotheka kusunga ma curve oyezedwa ngati Njira Yosungidwa. Kuti musunge kapindika wokhazikika kanikizani batani la njira yosungira ndikuyika dzina la njira, sankhani chikwatu ndikusunga ndi batani la Sungani. Njira zitha kutsegulidwa mumenyu ya Njira Zosungidwa patsamba loyambira. Kutsegula njira yosungidwa ya Protein Assay ikuwonetsa uthenga wokhala ndi mwayi wotsitsa kapena kuyezanso mayendedwe okhazikika.
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KUWERENGA
Kuyikirako kumatsimikiziridwa kudzera mu milingo ya absorbance yoperekedwa ndi curve yokhazikika kutengera kusankha kokwanira kwa ma curve kuphatikiza zosankha izi: kutsika kwa mzere, kutsika zero ndi 2nd order regression.
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CUSTOM APPS
Pali njira yopangira Makasitomala Okhazikika omwe amatha kukwezedwa ku NanoPhotometer® Go. Kuti mudziwe zambiri pakupanga mapulogalamu omwe amagwirizana ndi zosowa za kafukufuku aliyense, chonde lemberani ku Implen mwachindunji kuti akuthandizeni.
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ZOTSATIRA ZAKE
Chizindikiro cha Stored Results chimatsegula chikwatu cha zikwatu zomwe zili filezotsatira zomwe zasungidwa kale.
Kumanzere kwa chinsalu zonse zolembera / zosungira zomwe zilipo zikuwonetsedwa: NanoPhotometer®, Control Device, Network ndi/kapena USB flash drive (malingana ndi kupezeka). Kukankhira chikwatu chosungira kumawonetsa mafoda ang'onoang'ono a foda yosungirayi kumanzere ndi munthu payekha files kumanja. Kumanja kwa chophimba onse opulumutsidwa chifukwa files a chikwatu chosankhidwa akuwonetsedwa ndipo akhoza kutsegulidwa ndi kudina kwautali kapena kawiri. Mafoda amatha kuchotsedwa, kusinthidwanso, kusunthidwa kapena kukopera ndikukankhira pazithunzi. Ndizothekanso kufufuta, kutchulanso dzina, kusuntha kapena kukopera files pokankhira pa chithunzi. The file Njira ya chikwatu chosankhidwa ikuwonetsedwa pamwamba kumanja file dera. Chidziwitso: PDF ndi Excel files sangathe kutsegulidwa pa NanoPhotometer® Go. Files iyenera kusamutsidwa ku kompyuta kapena chipangizo komwe Excel kapena owerenga PDF aikidwa. Chidziwitso: Chipangizo chowongolera chimapezeka pa kompyuta, mapiritsi ndi mafoni a m'manja osati pa pulogalamu ya NanoPhotometer® Go. Kusamutsa kwa data kudzera pa Efaneti kapena WiFi onani tsamba 38 Data Transfer. Makopi osunga zobwezeretsera amasungidwa mufoda ya Autosave ya NanoPhotometer® Go (Zotsatira Zosungidwa/NanoPhotometer/Autosave) mpaka masiku khumi. Pambuyo masiku khumi autosave files amasamutsidwa kupita ku chikwatu chosungira zakale. Foda yosungiramo zinthu zakale imatha kupezeka kudzera pa NanoPhotometer® Go file seva. Zomwe zili mufoda yosungira zosungira sizichotsedwa zokha. Zomwe zili mufoda ya Autosave Archive zitha kuchotsedwa pogwiritsa ntchito batani lochitapo mu Zotsatira Zosungidwa. Onetsetsani kuti zosunga zobwezeretsera zapangidwa musanachotse zomwe zili mufoda ya Autosave Archive.
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NJIRA ZOSEKERA
Chizindikiro cha njira zosungidwa chimatsegula mndandanda wamafoda omwe ali ndi njira zosungidwa ndi wogwiritsa ntchito.
Kumanzere kwa chinsalu zonse zolembera / zosungira zomwe zilipo zikuwonetsedwa: NanoPhotometer®, Control Device, Network ndi/kapena USB flash drive (malingana ndi kupezeka). Kukankhira chikwatu chosungira kumawonetsa zikwatu zazing'ono za foda iyi kumanzere ndi munthu payekha files kumanja. Kumanja kwa chophimba onse opulumutsidwa njira files a chikwatu chosankhidwa akuwonetsedwa ndipo akhoza kutsegulidwa ndi kudina kwautali kapena kawiri. Pamwamba pa kumanja m'dera file Njira ya foda yosankhidwa ikuwonetsedwa. Zikwatu zatsopano zitha kupangidwa pokankhira pa . Mafoda amatha kuchotsedwa, kusinthidwanso, kusunthidwa kapena kukopera ndikukankhira pazithunzi. Ndizothekanso kufufuta, kutchulanso dzina, kusuntha kapena kukopera njira pokankhira chizindikirocho.
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5. KULAMBIRA
Zokonda pamakina zitha kukhazikitsidwa posankha zokonda patsamba lanyumba. Mndandanda wazokonda umaphatikizapo: Zambiri, Dyes, Mauthenga Ochenjeza, Network, Printer ndi CFR21. Zosankha zomwe mwasankha zomwe mwasankha zalembedwa pawindo lakumanja. Zindikirani: Zokonda sizipezeka pa mafoni am'manja okhala ndi skrini yochepera mainchesi 7.
ZAMBIRI
Kusankha General mu menyu ya Zokonda kumatsegula zenera kumanja kwa zokonda ndi zosankha zotsatirazi: Tsiku ndi Nthawi, Kuwonetsa, Za, Kusungirako ndi Kuwunikira S.ample Window (N50-Go).
TSIKU NDI NTHAWI
Mkati mwa Tsiku ndi Nthawi ndizotheka kukhazikitsa tsiku ndi nthawi yeniyeni ya NanoPhotometer® Go kapena kusintha zone yanthawi.
Kuti musinthe zone ya nthawi kapena tsiku ndi/kapena nthawi, kanikizani pagawo loyenera kuti mutsegule zosankha. Zosintha zosinthidwa zikuwonetsedwa pansipa gawo la zone ya nthawi. Kuti mugwiritse ntchito zosintha, NanoPhotometer® Go iyenera kuyambiranso. Yambitsani kuyambiranso mwa kukankhira pa Seti ndi Kuyambitsanso batani.
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NanoPhotometer® N50-Go/C40-Go User Manual Version 4.6.2 Zindikirani: Musasinthe tsiku ndi nthawi nthawi imodzi ndi nthawi. Onetsetsani kuti nthawi ya UTC yakhazikitsidwa bwino musanasinthe nthawi.
ONERANI
Kuwala: Kusintha kwa mawonekedwe opangidwa mkati
ZA
Pazotsatira zotsatirazi za NanoPhotometer® Go zikuwonetsedwa: NanoPhotometer® Version, Nambala ya Seri, Adilesi ya IP ya Efaneti, Adilesi ya IP ya WiFi, Mtundu wa Hardware, Mtundu wa Firmware, Nthawi & Tsiku Loyambitsa Mayeso ndi Mayeso Oyambitsa.
KUSINTHA
Imawonetsa mphamvu zonse zosungirako komanso malo aulere a NanoPhotometer® Go yosungirako.
KULALIRA SAMPLE WINDOW
Sinthani switch kuti muyatse/kuzimitsa kuwunikira kwa sample zenera (kwa N50-Go kokha)
DYES
Pali mndandanda wa zolemba za utoto zomwe zidakonzedweratu za utoto wa nucleic acid ndi utoto wa mapuloteni. Kuti musinthe pakati pa nucleic acid ndi mndandanda wa mapuloteni kanikizani mabatani a Nucleic Acid/Protein pamutu.
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NanoPhotometer® N50-Go/C40-Go User Manual Version 4.6.2
Utoto uliwonse uli ndi chizindikiro cha loko ( ) kutsogolo kwa dzina la utoto wosonyeza kuti utotowo ndi wokhoma ndipo sungathe kusinthidwa kapena chizindikiro chochotsa ( ). Njira yochotsera imapezeka kokha pamitundu yotsegulidwa osati yokonzedweratu. Kusankha dzina la utoto kumatsegula sikirini yatsopano yokhala ndi zambiri za utoto: dzina la utoto, utoto wothirira kwambiri (nm), utoto wodalira kutha kwa utoto (M-1 * cm-1), ndi dyedependent correction factor komanso njira yowonetsera. utoto pamndandanda wazogwiritsa ntchito (Nucleic Acid kapena Protein UV).
Zindikirani: Sizingatheke kuchotsa utoto pamndandanda wokhazikika wa fakitale; utoto wamtundu ukhoza kuchotsedwa ngati sunatsekedwe. Ndizotheka kuwonjezera utoto watsopano pamndandanda posankha batani + kuti muwonjezere utoto watsopano. Zenera lidzatsegulidwa pomwe mungathe kulowa: dzina la utoto, utoto wothirira kwambiri (nm), utoto wotengera kutha kwa utoto (M-1 * cm-1), ndi chowongolera chodalira utoto. Pali chosinthira chomwe chilipo kuti mutseke utoto kuti musachotse utoto mwangozi pamndandanda wa utoto.
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NanoPhotometer® N50-Go/C40-Go User Manual Version 4.6.2
MAU CHENJEZO
KULAMULIRA KWABWINO
Sinthani kusintha kuti muyatse/kuzimitsa Blank ControlTM ya NanoPhotometer® Go. Zindikirani: Blank Control TM ilipo pa njira zonse za NanoVolume (N50-Go).
SAMPLE QUALITY CONTROL
Ndi zotheka kusintha chapamwamba ndi m'munsi malire a chiŵerengero tcheru mauthenga chenjezo. Miyezo yofikira pa ma nucleic acid ndi: 260/230 chiyerekezo 1.8 A – 3 A ndi 260/280 chiyerekezo 1.65 A – 2.5 A. Mtengo wofikira wa mapuloteni a UV ndi: 260/280 chiŵerengero ndi 0.7 A.
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NanoPhotometer® N50-Go/C40-Go User Manual Version 4.6.2
NETWORK
Kusankha Network mu Zokonda menyu kumatsegula zenera kumanja kwa zokonda ndi izi: Zokonda pa Network, Zokonda za WLAN, File Seva Access ndi Network Foda.

Zolemba / Zothandizira

IMPLEN N50-Go Nano Photo Meter [pdf] Buku Logwiritsa Ntchito
N50-Go Nano Photo Meter, N50-Go, Nano Photo Meter, Photo Meter, Meter

IMPLEN N50-Go Nano Photo Meter User Manual

IMPLEN N50-Go Nano Photo Meter

Zambiri Zamalonda

Malangizo Ogwiritsira Ntchito Zogulitsa

Gulu la chitetezo IP20

ZAMBIRI

KULUMIKIZANA

MABUTANI

ZOYENERA KUYESA

KUYENZA PROTOCOL

Zolemba / Zothandizira

Maumboni

Siyani ndemanga

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