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Gold nanoparticles for 99mTc-doxorubicin delivery: formulation, in vitro characterization, comparative studies in vivo stability and biodistribution

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Abstract

The [99mTc]Tc-Doxorubicin–Gold Nanoparticles (99mTc-Dox-AuNPs) was formulated as a nanoradiopharmaceutical by different loading procedures to enhance tumor targeting. The formula F1 that was prepared by direct loading of pre-prepared [99mTc]Tc-Doxorubicin showed a reasonable in-vitro characterization values, high entrapment efficiency (92 ± 0.72%), acceptable in vitro release data and convenient in-vitro serum stability up to 24 h. F1 presented high tumor uptake (54%ID/g) with high Target/ Non Target (T/NT) ( ≈ 77) and Drug Target Efficiency percent (%DTE) above 100% at 0.5 h via intra-tumoral injection that prove the increasing of tumor targeting and in-vivo stability by direct loading for pre-prepared [99mTc]Tc-Doxorubicin.

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Acknowledgements

Associate Prof. Tamer M. Sakr expresses his grateful appreciation and thanks for International Atomic Energy Authority (IAEA), Austria for the international collaboration and funding this work under CRP No. F22064.

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Authors and Affiliations

  1. Labeled Compounds Department, Hot Labs Center, Egyptian Atomic Energy Authority, Cairo, 13759, Egypt

    D. M. El-Safoury, Ahmed B. Ibrahim & M. A. Motaleb

  2. Radioisotopes Production Facility, Second Egyptian Research Reactor Complex, Egyptian Atomic Energy Authority, Cairo, 13759, Egypt

    Ahmed B. Ibrahim & Tamer M. Sakr

  3. Pharmaceutics and Industrial Pharmacy Department, Faculty of Pharmacy, Cairo University, Cairo, 11562, Egypt

    D. A. El-Setouhy & O. M. Khowessah

  4. Radioactive Isotopes and Generator Department, Hot Labs Center, Egyptian Atomic Energy Authority, Cairo, 13759, Egypt

    Tamer M. Sakr

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  1. D. M. El-Safoury
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  4. O. M. Khowessah
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Correspondence to Ahmed B. Ibrahim or Tamer M. Sakr.

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El-Safoury, D.M., Ibrahim, A.B., El-Setouhy, D.A. et al. Gold nanoparticles for 99mTc-doxorubicin delivery: formulation, in vitro characterization, comparative studies in vivo stability and biodistribution. J Radioanal Nucl Chem 328, 325–338 (2021). https://doi.org/10.1007/s10967-021-07633-y

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