From traditional and folk medicine, the fruit of Capparis used as antiseptic for intestinal dysen... more From traditional and folk medicine, the fruit of Capparis used as antiseptic for intestinal dysentery and as protective for the liver from diseases in addition it is used as aphrodisiac and antihypertensive agent in addition as anticancer so as we know that natural product still a bank of new drug resources for the following reasons; these are a target for production by biotechnology. In addition, they are as a source of new lead compounds of novel chemical structure, which act as a tool for invention of new drug using nanoscince in medicine. There is a third reason as active ingredients of useful treatment divided from traditional medicine. As we know the drugs which manufacturing from bioactive, materials are cheap and available and not polluted as the chemical synthetic drugs and do not need-sophisticated technology. In this study we use fruits and leaves of Capparis dried and milled then extracted with alcohol 80% in addition deal with different organic solvent and get rid of ch...
1. Saudi Med J. 2004 Oct;25(10):1530-2. Acetylator phenotype in Iraqi patients with discoid lupus... more 1. Saudi Med J. 2004 Oct;25(10):1530-2. Acetylator phenotype in Iraqi patients with discoid lupus erythematosus. Najim RA, Farid YY, Shihab SA. Department of Pharmacology, College of Medicine, University of Baghdad, Baghdad, Iraq. rafidnajim@yahoo.com. ...
To evaluate seminal plasma coenzyme Q10 (CoQ10) levels and oxidative stress in patients with diff... more To evaluate seminal plasma coenzyme Q10 (CoQ10) levels and oxidative stress in patients with different types of male infertility. A case-control study was carried out in the Department of Chemistry and Biochemistry, College of Medicine, Al-Nahrain University, Baghdad, Iraq from the period of November 2004 to July 2006. Sixty patients with male infertility were recruited from Al-Kadhimia Teaching Hospital, Baghdad and included in this study. The male patients were categorized according to their seminal fluid parameters to oligozoospermia (n=32), azoospermia (n=22), and asthenozoospermia (n=6). All obtained results from infertile men groups were compared with age-matched healthy volunteers as control group consisting of 39 subjects. Seminal plasma samples were analyzed for CoQ10 by an improved high performance liquid chromatography (HPLC) method and for malondialdehyde (MDA) as an index of oxidative stress. The mean seminal plasma CoQ10 was 1.10+/-0.169 mg/L in oligozoospermia, 0.567+/-0.098 mg/L in azoospermia, 0.740+/-0.06 mg/L in patients with asthenozoospermia, and 1.652+/-0.139 mg/L in control group. The seminal plasma CoQ10 levels in all infertility groups showed a significant difference from the control group (p< or =0.0001). High significant increase (p < or =0.001) in the MDA levels was noted in the seminal plasma of oligozoospermia 11.37+/-1.64 micromole/L, azoospermia 13.87+/-1.62 micromole/L, and asthenozoospermia group 9.508+/-0.533 micromole/L whereas the level in the control group was 8.517+/- 0.622 mole/L. Seminal plasma CoQ10 was inversely and significantly correlated with MDA (r= -0.760; p=0.000). Elevated seminal plasma CoQ10 levels are associated directly with good semen parameters and inversely with the oxidative stress.
A high-performance liquid chromatographic assay for methotrexate and its metabolites, 7-hydroxy-m... more A high-performance liquid chromatographic assay for methotrexate and its metabolites, 7-hydroxy-methotrexate, 4-amino-4-deoxy-N10-methyl-pteroic acid and 7-hydroxy-4-amino-4-deoxy-N10-methyl-pteroic acid in the range 10 microg/l to 50 mg/l (2.2 x 10(-8) to 1.1. x 10(-4)M) has been developed using L-tryptophyl-L-glutamic acid as internal standard. Extraction was performed using an anion exchange resin (Dowex 1-X2) with subsequent ion-pair chromatography of the appropriate eluent fraction. The method has been found to be sensitive and precise for the analysis of both serum and urine, and may also be used for the quantitation of polyglutamyl metabolites.
A high-performance liquid chromatographic assay for methotrexate and its metabolites, 7-hydroxy-m... more A high-performance liquid chromatographic assay for methotrexate and its metabolites, 7-hydroxy-methotrexate, 4-amino-4-deoxy-N10-methyl-pteroic acid and 7-hydroxy-4-amino-4-deoxy-N10-methyl-pteroic acid in the range 10 microg/l to 50 mg/l (2.2 x 10(-8) to 1.1. x 10(-4)M) has been developed using L-tryptophyl-L-glutamic acid as internal standard. Extraction was performed using an anion exchange resin (Dowex 1-X2) with subsequent ion-pair chromatography of the appropriate eluent fraction. The method has been found to be sensitive and precise for the analysis of both serum and urine, and may also be used for the quantitation of polyglutamyl metabolites.
To identify the location of prolactin receptors in patients with uterine leiomyomas and their hos... more To identify the location of prolactin receptors in patients with uterine leiomyomas and their host myometrium as well as normal myometrium. A case control study was conducted at the College of Medicine Al-Nahrain University, Baghdad, Iraq during the period from 2004--2006. The samples were collected at Obstetrics and Gynecological Departments of 4 hospitals in Baghdad City (Al-Khadimiya Teaching Hospital, Al-Noor, Al-Kharch, and Al-Saadoon Hospital). Sections from large and small tumors (n=53) with their host myometriums and from normal myometriums (n=40) were stained immunohistochemically for prolactin receptors. Prolactin receptors were positively seen in all cases examined including patient and comparison tissues, in the form of dark brown staining. Staining was heterogenous and varied in intensity from one case to another and sometimes from one area to another in the same section. The increase in prolactin receptors in leiomyoma is expected given that the underlying host myometr...
From traditional and folk medicine, the fruit of Capparis used as antiseptic for intestinal dysen... more From traditional and folk medicine, the fruit of Capparis used as antiseptic for intestinal dysentery and as protective for the liver from diseases in addition it is used as aphrodisiac and antihypertensive agent in addition as anticancer so as we know that natural product still a bank of new drug resources for the following reasons; these are a target for production by biotechnology. In addition, they are as a source of new lead compounds of novel chemical structure, which act as a tool for invention of new drug using nanoscince in medicine. There is a third reason as active ingredients of useful treatment divided from traditional medicine. As we know the drugs which manufacturing from bioactive, materials are cheap and available and not polluted as the chemical synthetic drugs and do not need-sophisticated technology. In this study we use fruits and leaves of Capparis dried and milled then extracted with alcohol 80% in addition deal with different organic solvent and get rid of ch...
1. Saudi Med J. 2004 Oct;25(10):1530-2. Acetylator phenotype in Iraqi patients with discoid lupus... more 1. Saudi Med J. 2004 Oct;25(10):1530-2. Acetylator phenotype in Iraqi patients with discoid lupus erythematosus. Najim RA, Farid YY, Shihab SA. Department of Pharmacology, College of Medicine, University of Baghdad, Baghdad, Iraq. rafidnajim@yahoo.com. ...
To evaluate seminal plasma coenzyme Q10 (CoQ10) levels and oxidative stress in patients with diff... more To evaluate seminal plasma coenzyme Q10 (CoQ10) levels and oxidative stress in patients with different types of male infertility. A case-control study was carried out in the Department of Chemistry and Biochemistry, College of Medicine, Al-Nahrain University, Baghdad, Iraq from the period of November 2004 to July 2006. Sixty patients with male infertility were recruited from Al-Kadhimia Teaching Hospital, Baghdad and included in this study. The male patients were categorized according to their seminal fluid parameters to oligozoospermia (n=32), azoospermia (n=22), and asthenozoospermia (n=6). All obtained results from infertile men groups were compared with age-matched healthy volunteers as control group consisting of 39 subjects. Seminal plasma samples were analyzed for CoQ10 by an improved high performance liquid chromatography (HPLC) method and for malondialdehyde (MDA) as an index of oxidative stress. The mean seminal plasma CoQ10 was 1.10+/-0.169 mg/L in oligozoospermia, 0.567+/-0.098 mg/L in azoospermia, 0.740+/-0.06 mg/L in patients with asthenozoospermia, and 1.652+/-0.139 mg/L in control group. The seminal plasma CoQ10 levels in all infertility groups showed a significant difference from the control group (p< or =0.0001). High significant increase (p < or =0.001) in the MDA levels was noted in the seminal plasma of oligozoospermia 11.37+/-1.64 micromole/L, azoospermia 13.87+/-1.62 micromole/L, and asthenozoospermia group 9.508+/-0.533 micromole/L whereas the level in the control group was 8.517+/- 0.622 mole/L. Seminal plasma CoQ10 was inversely and significantly correlated with MDA (r= -0.760; p=0.000). Elevated seminal plasma CoQ10 levels are associated directly with good semen parameters and inversely with the oxidative stress.
A high-performance liquid chromatographic assay for methotrexate and its metabolites, 7-hydroxy-m... more A high-performance liquid chromatographic assay for methotrexate and its metabolites, 7-hydroxy-methotrexate, 4-amino-4-deoxy-N10-methyl-pteroic acid and 7-hydroxy-4-amino-4-deoxy-N10-methyl-pteroic acid in the range 10 microg/l to 50 mg/l (2.2 x 10(-8) to 1.1. x 10(-4)M) has been developed using L-tryptophyl-L-glutamic acid as internal standard. Extraction was performed using an anion exchange resin (Dowex 1-X2) with subsequent ion-pair chromatography of the appropriate eluent fraction. The method has been found to be sensitive and precise for the analysis of both serum and urine, and may also be used for the quantitation of polyglutamyl metabolites.
A high-performance liquid chromatographic assay for methotrexate and its metabolites, 7-hydroxy-m... more A high-performance liquid chromatographic assay for methotrexate and its metabolites, 7-hydroxy-methotrexate, 4-amino-4-deoxy-N10-methyl-pteroic acid and 7-hydroxy-4-amino-4-deoxy-N10-methyl-pteroic acid in the range 10 microg/l to 50 mg/l (2.2 x 10(-8) to 1.1. x 10(-4)M) has been developed using L-tryptophyl-L-glutamic acid as internal standard. Extraction was performed using an anion exchange resin (Dowex 1-X2) with subsequent ion-pair chromatography of the appropriate eluent fraction. The method has been found to be sensitive and precise for the analysis of both serum and urine, and may also be used for the quantitation of polyglutamyl metabolites.
To identify the location of prolactin receptors in patients with uterine leiomyomas and their hos... more To identify the location of prolactin receptors in patients with uterine leiomyomas and their host myometrium as well as normal myometrium. A case control study was conducted at the College of Medicine Al-Nahrain University, Baghdad, Iraq during the period from 2004--2006. The samples were collected at Obstetrics and Gynecological Departments of 4 hospitals in Baghdad City (Al-Khadimiya Teaching Hospital, Al-Noor, Al-Kharch, and Al-Saadoon Hospital). Sections from large and small tumors (n=53) with their host myometriums and from normal myometriums (n=40) were stained immunohistochemically for prolactin receptors. Prolactin receptors were positively seen in all cases examined including patient and comparison tissues, in the form of dark brown staining. Staining was heterogenous and varied in intensity from one case to another and sometimes from one area to another in the same section. The increase in prolactin receptors in leiomyoma is expected given that the underlying host myometr...
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