Papers by Peter M. Gresshoff
The CLAVATA pathway that regulates stem cell numbers of the shoot apical meristem has exclusively... more The CLAVATA pathway that regulates stem cell numbers of the shoot apical meristem has exclusively been studied in Arabidopsis; as such insight into other species is warranted. In this study, a GmCLV1A mutant (F-S562L) with altered lateral organ development, and two mutants of GmNARK, isolated from a Forrest M2 population (EMS-mutated soybean) were studied. GmCLV1A and GmNARK encode for LRR receptor kinases, and share 92% of protein sequence. While GmNARK is critical for systemic regulation of nodulation (new organ made on the root through symbiosis), we show that GmCLV1A functions locally and has no apparent function in nodulation or root development. However, a recessive, loss-of-function mutation (S562L) in a putative S-glycosylation site of GmCLV1A causes stem nodal identity alterations as well as flower and pod abnormalities (deformed flower and pod). The mutant also exhibits a homeotic phenotype, displaying abnormal leaf development/number, vein-derived leaf emergence, and a thick, faciated stem. The mutant phenotype is also temperature-sensitive. Interestingly, a novel truncated version of GmCLV1A was identified upstream of GmCLV1A that is absent from GmNARK, but is present upstream of the GmNARK orthologues, MtSUNN and PvNARK. Taken together, our findings indicate that GmCLV1A acts on shoot architecture, whereas GmNARK, functions in controlling nodule numbers.
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Plant shoot apical meristems (SAM) and root apical meri-stems (RAM) contain stem cells that form ... more Plant shoot apical meristems (SAM) and root apical meri-stems (RAM) contain stem cells that form overall-plant architecture. Mechanisms acting in these regions keep a balance between the stem cell population and differentiation. These mechanisms are well-studied in Arabidopsis, but little is known in the legume soybean (Glycine max (L.) Merr.). In Arabidopsis, the Leucine-rich repeat (LRR) Receptor kinase CLAVATA1 (CLV1) is a crucial regulator of this process in the SAM. In soybean, the receptor most similar to ATCLV1 is Gm-NARK, which is involved in nodulation control. In contrast, the homeologous partner of GmNARK in soybean, called GmCLV1A, appears to have no function in 'Autoregulation of nodulation' (AON) a role in regulating shoot architecture in the SAM. Here, the transcriptome of the shoot and root tip areas of a chemically induced and TILLING-selected Gm-CLV1A missense mutant, S562L, and its wild type, cultivar Forrest, were analysed to identify genes which are affected by impaired function of GmCLV1A. Among the differentially expressed genes identified, many were categorised as having a role in receptor kinase activity, transcription or defense/ stress-response. Molecular categories over-represented in the shoot tip of the mutant include those involved in hormone biosynthesis/activity and secondary metabolism, signalling, photosynthesis, and transport. Functional categories including those involved in polyamine metabolism, nucleotide metabolism, RNA regulation, protein targeting and protein degradation were under-represented in the shoot tip of the mutant. In the root tip, categories associated with signal
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Plant physiology, Jan 4, 2015
Legumes root nodules convert atmospheric nitrogen gas (N2) into ammonium through symbiosis with a... more Legumes root nodules convert atmospheric nitrogen gas (N2) into ammonium through symbiosis with a prokaryotic microsymbiont broadly called 'rhizobia'. Auxin signaling is required for determinant nodule development; however, the molecular mechanism of auxin-mediated nodule formation remains largely unknown. Here we show in soybean (Glycine max L. Merr) that microRNA (miRNA) miR167 acts as a positive regulator of lateral root organs, namely nodules and lateral roots. miR167c expression was up-regulated in the vasculature, pericycle and cortex of soybean roots following inoculation with Bradyrhizobium japonicum strain USDA110 (the microsymbiont). It was found to positively regulate nodule numbers directly by repressing the target genes GmARF8a and GmARF8b (homologous genes of Arabidopsis AtARF8 that encode auxin response factors). Moreover, the expression of miR167 and its targets were up- or down-regulated by auxin, respectively. The miR167-GmARF8 module also positively regula...
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... Peter M. Gresshoff, Artem E. Men, Anwar Hussain, Qunji Jiang, Dasharath Lohar, Irma L. Anders... more ... Peter M. Gresshoff, Artem E. Men, Anwar Hussain, Qunji Jiang, Dasharath Lohar, Irma L. Anderssen, Crina Zahari, Codruta-Andy Vasile, Madeleine Spencer ... 82, 588-590 Handberg K and Stougaard J (1992) Plant Journal 2, 487-496 Hussian AKM et al (1999) Plant and Cell ...
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Detection of very small amounts of RNA based on microdissection of plant tissue is essential for ... more Detection of very small amounts of RNA based on microdissection of plant tissue is essential for modern plant biology. Mass spectroscopy technology (MassARRAY) based on Sequenomtrade mark instrumentation was adapted to determine quickly and in a high-throughput fashion (by multiplexing) the absolute amounts of mRNA of closely related soybean genes. A sensitivity of 0.1 amol (10(-19)) was achieved, representing as few as 1,000 mRNA molecules. This methodology eliminates the use of housekeeping genes as reference standards and has multiple applications for plant functional genomics, such as the monitoring of individual expression of paralogous genes at ultra-low expression levels and/or in extremely small tissue samples.
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Papers by Peter M. Gresshoff