Pharmacological research : the official journal of the Italian Pharmacological Society, 2003
Overdose of acetaminophen, a widely used analgesic drug, can result in severe hepatotoxicity and ... more Overdose of acetaminophen, a widely used analgesic drug, can result in severe hepatotoxicity and is often fatal. This study was undertaken to examine the effects of arabic gum (AG), which is commonly used in processed foods, on acetaminophen-induced hepatotoxicity in mice. Mice were given arabic gum orally (100 g l(-1)) 5 days before a hepatotoxic dose of acetaminophen (500 mg kg(-1)) intraperitoneally. Arabic gum administration dramatically reduced acetaminophen-induced hepatotoxicity as evidenced by reduced serum alanine (ALT) and aspartate aminotransferase (AST) activities. Acetaminophen-induced hepatic lipid peroxidation was reduced significantly by arabic gum pretreatment. The protection offered by arabic gum does not appear to be caused by a decrease in the formation of toxic acetaminophen metabolites, which consumes glutathione, because arabic gum did not alter acetaminophen-induced hepatic glutathione depletion. Acetaminophen increased nitric oxide synthesis as measured by s...
The effects of Nω-nitro-L-arginine methylester (L-NAME) and L-arginine on cardiotoxicity that is ... more The effects of Nω-nitro-L-arginine methylester (L-NAME) and L-arginine on cardiotoxicity that is induced by doxorubicin (Dox) were investigated. A single dose of Dox 15 mg/kg i.p. induced cardiotoxicity, manifested biochemically by a significant elevation of serum creatine phosphokinase (CPK) activity (EC 2.7.3.2). Moreover, cardiotoxicity was further confirmed by a significant increase in lipid peroxides, measured as malon-di-aldehyde (MDA) in cardiac
Hepatocellular carcinoma accounts for about 80-90 % of all liver cancer and is the fourth most co... more Hepatocellular carcinoma accounts for about 80-90 % of all liver cancer and is the fourth most common cause of cancer mortality. Although there are many strategies for the treatment of liver cancer, chemoprevention seems to be the best strategy for lowering the incidence of this disease. Therefore, this study has been initiated to investigate whether thymoquinone (TQ), Nigella sativa derived-compound with strong antioxidant properties, supplementation could prevent initiation of hepatocarcinogenesis-induced by diethylnitrosamine (DENA), a potent initiator and hepatocarcinogen, in rats. Male Wistar albino rats were divided into 4 groups. Rats of group 1 received a single intraperitoneal (I.P.) injection of normal saline. Animals in group 2 were given TQ (4 mg/kg/day) in drinking water for 7 consecutive days. Rats of group 3 were injected with a single dose of DENA (200 mg/kg, I.P.). Animals in group 4 were received TQ and DENA. DENA significantly increased alanine transaminase (AL...
... Original Paper. Effect of L-Histidinol on Cisplatin Nephrotoxicity in the Rat Osama A. Badary... more ... Original Paper. Effect of L-Histidinol on Cisplatin Nephrotoxicity in the Rat Osama A. Badary, Mahmoud N. Nagi, Hussein A. Al-Sawaf, Mohammed Al-Harbi, Abdullah M. Al-Bekairi Department of Pharmacology, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia. ...
The modulating effect of thymoquinone (TQ) on benzo(a)pyrene (BP)-induced forestomach tumours was... more The modulating effect of thymoquinone (TQ) on benzo(a)pyrene (BP)-induced forestomach tumours was investigated in female Swiss albino mice, receiving oral administration of BP at a dose of 1 mg twice weekly for 4 weeks. Administration of 0.01% of TQ in drinking water 1 week before, during and after BP treatment until the end of the experiment resulted in significant suppression of BP-induced tumourigenesis when compared with the group receiving BP alone. TQ inhibited both BP-induced forestomach tumour incidence and multiplicity by 70% and 67%, respectively. Lipid peroxide accumulation and decreased glutathione (GSH) content and glutathione-S-transferase (GST) and DT diaphorase activities were observed in the liver of BP-treated tumour-bearing mice. TQ alone showed a significant induction in the enzyme activities of hepatic GST and DT diaphorase. Mice treated with TQ along with BP showed almost normal hepatic lipid peroxides and GSH levels, and normal enzyme activities compared to the control group. The present data may indicate the potential of TQ, the main constituent of the volatile oil of Nigella sativa seed, as a powerful chemopreventive agent against BP-induced forestomach tumours in mice. The possible modes of action of TQ may be through its antioxidant and anti-inflammatory activities, coupled with enhancement of detoxification processes.
Pharmacological research : the official journal of the Italian Pharmacological Society, 2003
Overdose of acetaminophen, a widely used analgesic drug, can result in severe hepatotoxicity and ... more Overdose of acetaminophen, a widely used analgesic drug, can result in severe hepatotoxicity and is often fatal. This study was undertaken to examine the effects of arabic gum (AG), which is commonly used in processed foods, on acetaminophen-induced hepatotoxicity in mice. Mice were given arabic gum orally (100 g l(-1)) 5 days before a hepatotoxic dose of acetaminophen (500 mg kg(-1)) intraperitoneally. Arabic gum administration dramatically reduced acetaminophen-induced hepatotoxicity as evidenced by reduced serum alanine (ALT) and aspartate aminotransferase (AST) activities. Acetaminophen-induced hepatic lipid peroxidation was reduced significantly by arabic gum pretreatment. The protection offered by arabic gum does not appear to be caused by a decrease in the formation of toxic acetaminophen metabolites, which consumes glutathione, because arabic gum did not alter acetaminophen-induced hepatic glutathione depletion. Acetaminophen increased nitric oxide synthesis as measured by s...
The effects of Nω-nitro-L-arginine methylester (L-NAME) and L-arginine on cardiotoxicity that is ... more The effects of Nω-nitro-L-arginine methylester (L-NAME) and L-arginine on cardiotoxicity that is induced by doxorubicin (Dox) were investigated. A single dose of Dox 15 mg/kg i.p. induced cardiotoxicity, manifested biochemically by a significant elevation of serum creatine phosphokinase (CPK) activity (EC 2.7.3.2). Moreover, cardiotoxicity was further confirmed by a significant increase in lipid peroxides, measured as malon-di-aldehyde (MDA) in cardiac
Hepatocellular carcinoma accounts for about 80-90 % of all liver cancer and is the fourth most co... more Hepatocellular carcinoma accounts for about 80-90 % of all liver cancer and is the fourth most common cause of cancer mortality. Although there are many strategies for the treatment of liver cancer, chemoprevention seems to be the best strategy for lowering the incidence of this disease. Therefore, this study has been initiated to investigate whether thymoquinone (TQ), Nigella sativa derived-compound with strong antioxidant properties, supplementation could prevent initiation of hepatocarcinogenesis-induced by diethylnitrosamine (DENA), a potent initiator and hepatocarcinogen, in rats. Male Wistar albino rats were divided into 4 groups. Rats of group 1 received a single intraperitoneal (I.P.) injection of normal saline. Animals in group 2 were given TQ (4 mg/kg/day) in drinking water for 7 consecutive days. Rats of group 3 were injected with a single dose of DENA (200 mg/kg, I.P.). Animals in group 4 were received TQ and DENA. DENA significantly increased alanine transaminase (AL...
... Original Paper. Effect of L-Histidinol on Cisplatin Nephrotoxicity in the Rat Osama A. Badary... more ... Original Paper. Effect of L-Histidinol on Cisplatin Nephrotoxicity in the Rat Osama A. Badary, Mahmoud N. Nagi, Hussein A. Al-Sawaf, Mohammed Al-Harbi, Abdullah M. Al-Bekairi Department of Pharmacology, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia. ...
The modulating effect of thymoquinone (TQ) on benzo(a)pyrene (BP)-induced forestomach tumours was... more The modulating effect of thymoquinone (TQ) on benzo(a)pyrene (BP)-induced forestomach tumours was investigated in female Swiss albino mice, receiving oral administration of BP at a dose of 1 mg twice weekly for 4 weeks. Administration of 0.01% of TQ in drinking water 1 week before, during and after BP treatment until the end of the experiment resulted in significant suppression of BP-induced tumourigenesis when compared with the group receiving BP alone. TQ inhibited both BP-induced forestomach tumour incidence and multiplicity by 70% and 67%, respectively. Lipid peroxide accumulation and decreased glutathione (GSH) content and glutathione-S-transferase (GST) and DT diaphorase activities were observed in the liver of BP-treated tumour-bearing mice. TQ alone showed a significant induction in the enzyme activities of hepatic GST and DT diaphorase. Mice treated with TQ along with BP showed almost normal hepatic lipid peroxides and GSH levels, and normal enzyme activities compared to the control group. The present data may indicate the potential of TQ, the main constituent of the volatile oil of Nigella sativa seed, as a powerful chemopreventive agent against BP-induced forestomach tumours in mice. The possible modes of action of TQ may be through its antioxidant and anti-inflammatory activities, coupled with enhancement of detoxification processes.
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Papers by Mahmoud Nagi