Papers by Miguel Angel Jiménez-Clavero
International journal of environmental research and public health, Jan 10, 2018
In the context of One Health, there is presently an effort to integrate surveillance of human, an... more In the context of One Health, there is presently an effort to integrate surveillance of human, animal, entomological, and environmental sectors. This aims to strengthen the prevention of, and preparedness against, arbovirus infections, also in the light of environmental and climate changes that could increase the risk of transmission. However, criteria to define integrated surveillance, and to compare different systems, still need to be identified and tested. We conducted a scoping review to identify and examine surveillance systems for West Nile virus (WNV), chikungunya virus (CHKV), dengue virus (DENV), and Rift Valley fever virus (RVFV), which involve human, animal, entomological, and environmental sectors. We analyzed findings using a conceptual framework we developed for this purpose. The review highlights that the criteria proposed in the conceptual framework to describe integrated surveillance are consistently reported in the context of studies and programs related to integra...
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Journal of General Virology
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Journal of Virological Methods, 2013
An increase in activity of two mosquito-borne flaviviruses, West Nile virus (WNV) and Usutu virus... more An increase in activity of two mosquito-borne flaviviruses, West Nile virus (WNV) and Usutu virus (USUV), has been reported in Europe in recent years. The current epidemiological situation calls for RT-PCR methods that are able to detect not only the widespread lineage 1 (L1) WNV, but also lineage 2 (L2) WNV. In addition, the presence in Europe of the closely related USUV requires methods that can identify these three flaviviruses and permit an efficient and accurate differential diagnosis. Here we describe a new one-step real-time multiplex RT-PCR that detects and differentiates efficiently WNV-L1, WNV-L2 and USUV in a single reaction. The assay is based on different sets of primers and fluorogenic probes specific to each virus that are labelled with selective, non-overlapping fluorogen-quencher pairs. This enables the fluorescence emitted by each probe, characterized by distinct wavelengths, to be differentiated. This multiplex assay was very sensitive to all of the target viruses; in addition, there were no cross-reactions between the viruses and the assay did not react to any other phylogenetically or symptomatically related viruses. Quantitation was enabled through the use of in vitro-transcribed RNAs developed specifically for each virus as copy number standards. This new assay was validated using different types of experimental and field samples.
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The American Journal of …, 1978
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Journal of General Virology, 2014
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Journal of Wildlife …, Jan 1, 2010
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Journal of General Virology, 2004
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Animal Health Research Reviews, 2000
Swine vesicular disease is a highly contagious disease of pigs that is caused by an enterovirus o... more Swine vesicular disease is a highly contagious disease of pigs that is caused by an enterovirus of the family Picornaviridae. The virus is a relatively recent derivative of the human coxsackievirus B5, with which it has high molecular and antigenic homology. The disease is not severe, and affected animals usually show moderate general weakening and slight weight loss that is recovered in few days, as well as vesicular lesions in the mucosa of the mouth and nose and in the interdigital spaces of the feet. However, the similarity of these lesions to those caused by foot-and-mouth disease virus has led to the inclusion of this virus in list A of the Office International des Epizooties. The disease has been eradicated in the European Union except in Italy, where it is considered endemic in the south. Nevertheless, as occasional outbreaks still appear and must be eliminated rapidly, European countries are on the alert and farms are monitored routinely for the presence of the virus. This circumstance has led to a considerable effort to study the pathology of the disease and the molecular biology and antigenicity of the virus, andto the development of optimized methods for the diagnosis of the infection.
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Eurosurveillance, 2015
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Birds are part of our environment. Of the myriad of viruses that affect birds, only a small porti... more Birds are part of our environment. Of the myriad of viruses that affect birds, only a small portion is zoonotic, i.e., can infect and cause disease in humans.Among them, there are two groups: the first is constituted by avian influenza (or flu) viruses, mainly airborne transmitted; and the second is constituted by certain viruses transmitted by arthropod bites, generally referred to as arboviruses, belonging to the Flaviviridae family, genus Flavivirus, and Togaviridae family, genus Alphavirus.These include important human pathogens such as West Nile virus, Japanese encephalitis virus, Sindbis virus and Eastern equine encephalitis virus. Many of these zoonotic viruses with avian reservoir have caused emergency episodes recently, as the case of avian influenza virus subtypes H5N1 and H7N9, both originated in Asia, or West Nile virus, which in the past two decades has reached a worldwide distribution, being currently considered the most widespread arbovirus on Earth.These two cases hi...
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22.161. Old world vs. new world West Nile virus: is there any difference? M. A. Jimenez-Clavero1,... more 22.161. Old world vs. new world West Nile virus: is there any difference? M. A. Jimenez-Clavero1, E. Perez-Ramirez1, F. Llorente1, J. Del Amo1, N. Nowotny2, R. Soriguer3, J. Figuerola4 1INIA, Valdeolmos, Spain, 2University of Veterinary Medicine, Vienna,Austria, 3Sevilla, Seville, Spain, 4Estación Biológica de Doñana, CSIC,Seville, Spain Purpose: West Nile virus (WNV) emerged in New York in 1999, and subsequently spread throughout the Americas, with an unprecedented speed and virulence for humans, horses and wild birds. Almost simultaneously, WNV re-emerged in Europe and the Mediterranean area after an absence of almost three decades, but showing more limited spread capacity and virulence, and lower bird mortality. We and others have demonstrated recently that European wild bird species are susceptible to disease and death by WNV infection. Therefore, the strains circulating in each Continent could account for this different behaviour. We have shown previously that European WNV stra...
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Virus Research, 1998
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Acta crystallographica. Section D, Biological crystallography, 2003
Three different crystal forms of the swine vesicular disease virus (SVDV), isolate SPA/2/'93,... more Three different crystal forms of the swine vesicular disease virus (SVDV), isolate SPA/2/'93, were obtained by the hanging-drop vapour-diffusion technique using ammonium sulfate and sodium/potassium phosphate as precipitants. Monoclinic crystals, space group C2, with unit-cell parameters a = 473.7, b = 385.3, c = 472.8 A, beta = 100.4 degrees, contain one virus pArticle in the crystal asymmetric unit and diffract to 3.0 A resolution. A second type of crystals had a cubic morphology and diffracted beyond 2.6 A resolution. These crystals belong to a primitive orthorhombic space group, with unit-cell parameters a = 319.6, b = 353.8, c = 377.7 A, and contain half a virus pArticle in the asymmetric unit. A third type of crystals, with a prismatic shape and belonging to space group I222, was also obtained under similar crystallization conditions. These latter crystals, with unit-cell parameters a = 318.3, b = 349.9, c = 371.7 A, diffract to at least 3.0 A resolution and contain 15 pro...
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The Journal of biological chemistry, Jan 21, 1994
Factor J (FJ) is a cationic glycoprotein with inhibitory activity in C1, the first component of t... more Factor J (FJ) is a cationic glycoprotein with inhibitory activity in C1, the first component of the classical complement pathway. This study demonstrates that FJ is able to regulate the activity of the alternative complement pathway. FJ inhibits the generation of fluid-phase and cell-bound alternative pathway C3 convertase, C3b,Bb (C3-cleaving enzyme). Thus, FJ interferes with the generation of alternative pathway C3 convertase when sheep erythrocytes bearing antibody and activated C3 and C4 (EAC4b,3b) are incubated with the individual complement components, factors B, D, and P. FJ accelerates the decay of C3 convertase with a time course similar to that of factor H, and when both regulators are present together, the decay of enzyme activity is faster than when they are added separately. Furthermore, FJ is able to inhibit the cleavage of C3 by factor B in a fluid-phase assay. FJ prevents the initiation of alternative pathway activation in "more stabilized systems" with wel...
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PLoS neglected tropical diseases, 2015
The family Flaviviridae, genus Flavivirus, holds many of the world's most prevalent arboviral... more The family Flaviviridae, genus Flavivirus, holds many of the world's most prevalent arboviral diseases that are also considered the most important travel related arboviral infections. In most cases, flavivirus diagnosis in travelers is primarily based on serology as viremia is often low and typically has already been reduced to undetectable levels when symptoms set in and patients seek medical attention. Serological differentiation between flaviviruses and the false-positive results caused by vaccination and cross-reactivity among the different species, are problematic for surveillance and diagnostics of flaviviruses. Their partially overlapping geographic distribution and symptoms, combined with increase in travel, and preexisting antibodies due to flavivirus vaccinations, expand the need for rapid and reliable multiplex diagnostic tests to supplement currently used methods. We describe the development of a multiplex serological protein microarray using recombinant NS1 proteins...
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ANTONIO TENORIO ha trabajado como virólogo de salud pública en el actual Centro Nacional de Micro... more ANTONIO TENORIO ha trabajado como virólogo de salud pública en el actual Centro Nacional de Microbiología (CNM-ISCIII) hasta que un proceso tumoral le apartó de sus responsabilidades. Durante los últimos años sus esfuerzos han ido dirigidos a impulsar la investigación en zoonosis víricas en España, en Europa y en Iberoamérica mediante el establecimiento de redes colaborativas multidis-ciplinares. MIGUEL ÁNGEL JIMÉNEZ-CLAVERO desarrolla su labor como virólogo en el Centro de Alta Bioseguridad del Centro de Investigación en Sanidad Animal (CISA), perteneciente al Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA). Su trabajo actual se centra en el diagnóstico y la vigilancia de las enfermedades víricas animales con alta capacidad de dispersión o especial virulencia, incluyendo zoonosis víricas emergentes transmitidas por vectores.
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Papers by Miguel Angel Jiménez-Clavero