Background:Azoxymethane (AOM) induce colon cancer in experimental animal; in mechanism that is me... more Background:Azoxymethane (AOM) induce colon cancer in experimental animal; in mechanism that is mediated by glutathione (GSH) depletion and impairing total antioxidant capacity (TAC) in colonic cells. Objective: This study investigated the effectiveness of, β-Casein (extracted from camel milk) in the prevention of AOM-induced colon cancer.Method: The experimental animals received AOM intraperitonial injection in the presence or absence of oral supplementation of β-Casein, meanwhile the control group was fed basal diet. After 8 weeks, the rats were sacrificed and the colon tissues were examined for aberrant crypt foci (ACF) enumeration and for biochemical measurements of GSH and TAC. Results: Our data show that AOM-induced GSH depletion that was associated with an impaired TAC in colonic mucosal tissues and the ACF counts were significantly high. The concomitant treatments of AOM-injected rats with β-Casein reduce the AOM-mediated carcinogenesis. Conclusion: β-Casein exhibited anti-carcinogenic effect again...
Clinical studies have claimed in general to show a correlation between high consumption of phenol... more Clinical studies have claimed in general to show a correlation between high consumption of phenolic antioxidants and reduced risks of certain types of cancer. The main antioxidant activity that has been associated with polyphenols is the ability to scavenge free radicals. Prevention of cancer may be accomplished through primary prevention, secondary prevention, or a combination of these approaches. The objective of primary prevention is to prohibit effective contact of a carcinogenic agent with a susceptible target in the human body, so that the sequence of events that culminates in the occurrence of clinical cancer does not begin or is aborted at the start. Primary prevention of cancer might be implemented by fortifying the human diet with an effective dietary chemoprevention, among this approach is the date pit extract.
Background Azoxymethane (AOM) is a potent carcinogenic agent commonly used to induce colon cancer... more Background Azoxymethane (AOM) is a potent carcinogenic agent commonly used to induce colon cancer in rats; the cytotoxicity of AOM is considered to mediate oxidative stress. This study investigated the chemopreventive effect of three natural extracts [pomegranate peel extract (PomPE), papaya peel extract (PapPE) and seaweed extract (SE)] against AOM-induced oxidative stress and carcinogenesis in rat colon. Methods Eighty Sprague–Dawley rats (aged 4 weeks) were randomly divided into 8 groups (10 rats/group). Control group was fed a basal diet; AOM-treated group was fed a basal diet and received AOM intraperitonial injections for two weeks at a dose of 15 mg/kg bodyweight, whereas the other six groups were received oral supplementation of PomPE, PapPE or SE, in the presence or absence of AOM injection. All animals were continuously fed ad-libitum until aged 16 weeks, then all rats were sacrificed and the colon tissues were examined microscopically for pathological changes and aberrant...
Dates’ color is known to play a crucial role in determining the value and quality of the fruit. T... more Dates’ color is known to play a crucial role in determining the value and quality of the fruit. The color changes from the natural accepted golden color to unfavorable dark brown color during storage. In this study, the effect of different color preservation methods (modified atmosphere packaging, cold storage (4°C), sulfur dioxide gas (SO2), and blanching) and its relation to darkening due to action of the browning enzymes and melanin production were investigated. Polyphenol oxidase was shown to be active in all treatments except the samples treated with SO2 gas and steam blanching for ten minutes. Likewise, peroxidase activity showed a similar trend in all samples, but a decrease in activity was observed in sulfated samples and total inactivation in steam blanching for ten minutes. Moreover, sulfated samples have shown improvement in color compared to all other treatments, whereas the steamed samples showed the highest color deterioration. Concurrently, melanin content increased i...
Seaweed contained health functional polysaccharides and polyphenols. Five extracts were prepared ... more Seaweed contained health functional polysaccharides and polyphenols. Five extracts were prepared from red seaweed Melanothamnus somalensis; two of these were aqueous at 20°C (F1) and 70°C (F2), followed by acid (F3), alkali (F4) and acid-washed (F5) treatments. Molecular characteristics of extracts were measured by high-performance liquid chromatography and Fourier transform infrared (FTIR) analyses. Health functionality was determined by sulfate and polyphenol contents, and thermal characteristics were determined by modulated differential scanning calorimetry. Extract F3 contained the highest levels of sulfate followed by F2, F1, F4 and F5, respectively. Similarly, F3 contained the highest polyphenol followed by F4, F2, F1 and F5, respectively. Molecular weight distribution of F1 showed wider distribution of sizes (MW: 1.0 × 103 – 3.0 × 107), and F5 showed a sharp peak (MW: 3.1 × 103); whereas F2, F3 and F4 indicated bimodal distribution. FTIR indicated that all fractions contained...
Hypnea bryoides collected from the Arabian Sea on the southern coast of Oman was investigated for... more Hypnea bryoides collected from the Arabian Sea on the southern coast of Oman was investigated for κ-carrageenan optimal extraction conditions. The effects of different conditions of alkali treatment (4, 6, and 8% w/v NaOH), temperatures (70, 75, and 80°C), and time (2, 2.75, 3.5 hours) on carrageenan yield and chemical and thermal properties were evaluated. Yield was significantly affected by alkaline concentration and temperature, with highest value of 26.74 ± 5.01%. Molecular weights of the extracted carrageenan were significantly reduced by increased temperatures and ranged from 5.95 ± 0.49 × 105 Da to 13.90 ± 0.14 × 105. FTIR showed that samples under all extraction conditions were similar and confirmed the presence of κ-carrageenan with no traces of μ-precursor. Sulfate content was also significantly reduced by alkaline concentration (from 4% to 6%) and ranged from 7.62 ± 5.52% to 17.02 ± 0.14. Thermal properties showed more sensitivity towards temperature and alkaline strength...
Abstract This paper summarizes the application of stoichiometric analytical approaches to quantit... more Abstract This paper summarizes the application of stoichiometric analytical approaches to quantitative IR analysis and describes the development of a rapid and sensitive Fourier transform infrared (FTIR) method using such an approach for the determination of low ...
Background:Azoxymethane (AOM) induce colon cancer in experimental animal; in mechanism that is me... more Background:Azoxymethane (AOM) induce colon cancer in experimental animal; in mechanism that is mediated by glutathione (GSH) depletion and impairing total antioxidant capacity (TAC) in colonic cells. Objective: This study investigated the effectiveness of, β-Casein (extracted from camel milk) in the prevention of AOM-induced colon cancer.Method: The experimental animals received AOM intraperitonial injection in the presence or absence of oral supplementation of β-Casein, meanwhile the control group was fed basal diet. After 8 weeks, the rats were sacrificed and the colon tissues were examined for aberrant crypt foci (ACF) enumeration and for biochemical measurements of GSH and TAC. Results: Our data show that AOM-induced GSH depletion that was associated with an impaired TAC in colonic mucosal tissues and the ACF counts were significantly high. The concomitant treatments of AOM-injected rats with β-Casein reduce the AOM-mediated carcinogenesis. Conclusion: β-Casein exhibited anti-carcinogenic effect again...
Clinical studies have claimed in general to show a correlation between high consumption of phenol... more Clinical studies have claimed in general to show a correlation between high consumption of phenolic antioxidants and reduced risks of certain types of cancer. The main antioxidant activity that has been associated with polyphenols is the ability to scavenge free radicals. Prevention of cancer may be accomplished through primary prevention, secondary prevention, or a combination of these approaches. The objective of primary prevention is to prohibit effective contact of a carcinogenic agent with a susceptible target in the human body, so that the sequence of events that culminates in the occurrence of clinical cancer does not begin or is aborted at the start. Primary prevention of cancer might be implemented by fortifying the human diet with an effective dietary chemoprevention, among this approach is the date pit extract.
Background Azoxymethane (AOM) is a potent carcinogenic agent commonly used to induce colon cancer... more Background Azoxymethane (AOM) is a potent carcinogenic agent commonly used to induce colon cancer in rats; the cytotoxicity of AOM is considered to mediate oxidative stress. This study investigated the chemopreventive effect of three natural extracts [pomegranate peel extract (PomPE), papaya peel extract (PapPE) and seaweed extract (SE)] against AOM-induced oxidative stress and carcinogenesis in rat colon. Methods Eighty Sprague–Dawley rats (aged 4 weeks) were randomly divided into 8 groups (10 rats/group). Control group was fed a basal diet; AOM-treated group was fed a basal diet and received AOM intraperitonial injections for two weeks at a dose of 15 mg/kg bodyweight, whereas the other six groups were received oral supplementation of PomPE, PapPE or SE, in the presence or absence of AOM injection. All animals were continuously fed ad-libitum until aged 16 weeks, then all rats were sacrificed and the colon tissues were examined microscopically for pathological changes and aberrant...
Dates’ color is known to play a crucial role in determining the value and quality of the fruit. T... more Dates’ color is known to play a crucial role in determining the value and quality of the fruit. The color changes from the natural accepted golden color to unfavorable dark brown color during storage. In this study, the effect of different color preservation methods (modified atmosphere packaging, cold storage (4°C), sulfur dioxide gas (SO2), and blanching) and its relation to darkening due to action of the browning enzymes and melanin production were investigated. Polyphenol oxidase was shown to be active in all treatments except the samples treated with SO2 gas and steam blanching for ten minutes. Likewise, peroxidase activity showed a similar trend in all samples, but a decrease in activity was observed in sulfated samples and total inactivation in steam blanching for ten minutes. Moreover, sulfated samples have shown improvement in color compared to all other treatments, whereas the steamed samples showed the highest color deterioration. Concurrently, melanin content increased i...
Seaweed contained health functional polysaccharides and polyphenols. Five extracts were prepared ... more Seaweed contained health functional polysaccharides and polyphenols. Five extracts were prepared from red seaweed Melanothamnus somalensis; two of these were aqueous at 20°C (F1) and 70°C (F2), followed by acid (F3), alkali (F4) and acid-washed (F5) treatments. Molecular characteristics of extracts were measured by high-performance liquid chromatography and Fourier transform infrared (FTIR) analyses. Health functionality was determined by sulfate and polyphenol contents, and thermal characteristics were determined by modulated differential scanning calorimetry. Extract F3 contained the highest levels of sulfate followed by F2, F1, F4 and F5, respectively. Similarly, F3 contained the highest polyphenol followed by F4, F2, F1 and F5, respectively. Molecular weight distribution of F1 showed wider distribution of sizes (MW: 1.0 × 103 – 3.0 × 107), and F5 showed a sharp peak (MW: 3.1 × 103); whereas F2, F3 and F4 indicated bimodal distribution. FTIR indicated that all fractions contained...
Hypnea bryoides collected from the Arabian Sea on the southern coast of Oman was investigated for... more Hypnea bryoides collected from the Arabian Sea on the southern coast of Oman was investigated for κ-carrageenan optimal extraction conditions. The effects of different conditions of alkali treatment (4, 6, and 8% w/v NaOH), temperatures (70, 75, and 80°C), and time (2, 2.75, 3.5 hours) on carrageenan yield and chemical and thermal properties were evaluated. Yield was significantly affected by alkaline concentration and temperature, with highest value of 26.74 ± 5.01%. Molecular weights of the extracted carrageenan were significantly reduced by increased temperatures and ranged from 5.95 ± 0.49 × 105 Da to 13.90 ± 0.14 × 105. FTIR showed that samples under all extraction conditions were similar and confirmed the presence of κ-carrageenan with no traces of μ-precursor. Sulfate content was also significantly reduced by alkaline concentration (from 4% to 6%) and ranged from 7.62 ± 5.52% to 17.02 ± 0.14. Thermal properties showed more sensitivity towards temperature and alkaline strength...
Abstract This paper summarizes the application of stoichiometric analytical approaches to quantit... more Abstract This paper summarizes the application of stoichiometric analytical approaches to quantitative IR analysis and describes the development of a rapid and sensitive Fourier transform infrared (FTIR) method using such an approach for the determination of low ...
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Papers by Ahmed Al-alawi