Papers by Abdelaziz Souli
BMC Complementary and Alternative Medicine, 2015
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RSC Adv., 2015
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RSC Adv., 2015
ABSTRACT We aimed in the present study to investigate the protective effect of myrtle (Myrtus com... more ABSTRACT We aimed in the present study to investigate the protective effect of myrtle (Myrtus communis L.) berries seeds aqueous extract (MBSAE) on acetic acid (AA)-induced colitis in rat as well as the mechanism implicated in this coli-protection. The use of LC/MS technique allowed us to identify 18 phenolic compounds in MBSAE. Secondly, we found that MBSAE inhibited luminol-amplified chemiluminescence of resting neutrophils and N-formyl-methionylleucyl-phenylalanine (fMLF) or phorbolmyristate acetate (PMA) stimulated neutrophils, in a dose-dependent manner. MBSAE had no effect on superoxide anion, but it inhibited H2O2 production in cell free system stimulated with horseradish peroxydase (HRPO) and MPO release from neutrophils. In vivo, the pre-treatment of rats with sulfasalazine (100 mg/kg) and MBSAE (25, 50, and 100 mg/kg) significantly reduced the AA-induced colonic mucosa lesion as well as histopathology changes. MBSAE counteracted AA-induced lipid peroxidation and ant antioxidant enzymes activities depletion such as superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx). We also found that myrtle extract inhibited the increase of plasma scavenging activity (PSA) and preserved non-enzymatic antioxidants content such a sulfhydryl groups (-SH) and reduced glutathione (GSH). More importantly, acetic acid administration increased colonic hydrogen peroxide (H2O2), free iron and calcium levels, while the MBSAE pre-treatment reversed all the effects on these intracellular mediators. In conclusion, our data suggests that MBSAE exerted a potential protective effect against AA-induced injury and oxidative stress in rat colon. This coli-protection might be related to in part, to its antioxidant and ROS scavenging activities or by negatively regulating Fenton reaction components such as H2O2 and free iron, which are known to lead to cytotoxicity mediated by intracellular calcium deregulation.
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General physiology and biophysics, Jan 27, 2015
The present study assessed the chemical composition, antioxidant properties, and hepatoprotective... more The present study assessed the chemical composition, antioxidant properties, and hepatoprotective effects of subacute pre-treatment with chamomile (Matricaria recutita L.) decoction extract (CDE) against ethanol (EtOH)-induced oxidative stress in rats. The colorimetric analysis demonstrated that the CDE is rich in total polyphenols, total flavonoids and condensed tannins, and exhibited an important in vitro antioxidant activity. The use of LC/MS technique allowed us to identify 10 phenolic compounds in CDE. We found that CDE pretreatment, in vivo, protected against EtOH-induced liver injury evident by plasma transaminases activity and preservation of the hepatic tissue structure. The CDE counteracted EtOH-induced liver lipoperoxidation, preserved thiol -SH groups and prevented the depletion of antioxidant enzyme activity of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx). We also showed that acute alcohol administration increased tissue and plasma hydroge...
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Journal of Medicinal Food, 2015
The present study was undertaken to determine whether total and methanol juice extracts of two Tu... more The present study was undertaken to determine whether total and methanol juice extracts of two Tunisian Pomegranate (Punica granatum L.) varieties (Garsi and Gabsi) protect against diarrhea as well as their effects on gastrointestinal transit (GIT) in healthy rats. In this respect, male Wistar rats were used and divided into control- and pomegranate-treated groups. The antidiarrheal activity was evaluated using the castor oil-induced diarrhea method and the GIT was assessed using charcoal meal. Our results showed that total and methanol P. granatum juice extracts produced a significant dose-dependent protection against castor oil-induced diarrhea. Pomegranate extracts and juice also decreased the GIT significantly and dose dependently. Importantly, the Garsi variety appeared to be more effective than the Gabsi variety on these two parameters. These findings suggest that pomegranate extracts have a potent antidiarrheal property in rats confirming their efficiency in the Tunisian traditional medicine.
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Toxicology and Industrial Health, 2013
The present study was undertaken to determine whether subacute treatment with aqueous extract of ... more The present study was undertaken to determine whether subacute treatment with aqueous extract of carob (Ceratonia siliqua L.) pods (AECPs) protects against ethanol (EtOH)-induced oxidative stress in rat liver. Animals were divided into four groups: control, carob, EtOH and EtOH + carob. Wistar rats were intraperitoneally pretreated with AECP (600 mg/kg body weight (bw)) during 7 days and intoxicated for 6 h by acute oral administration of EtOH (6 g/kg bw) 24 h after the last injection. We found that acute administration of EtOH leads to hepatotoxicity as monitored by the increase in the levels of hepatic marker aspartate aminotransferase and alanine aminotransferase as well as hepatic tissue injury. EtOH also increased the formation of malondialdehyde in the liver, indicating an increase in lipid peroxidation and depletion of antioxidant enzyme activities as superoxide dismutase, catalase and glutathione peroxidase. Subacute carob pretreatment prevented all the alterations induced by EtOH and returned their levels to near normal. Importantly, we showed that acute alcohol increased hepatic and plasmatic hydrogen peroxide and free iron levels. The carob pretreatment reversed EtOH effects to near control levels. These data suggest that carob could have a beneficial effect in inhibiting the oxidative damage induced by acute EtOH administration and that its mode of action may involve an opposite effect on plasma and tissue-free iron accumulation. Indeed, carob can be offered as a food additive to protect against EtOH-induced oxidative damage.
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RSC Adv., 2014
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Journal of Ethnopharmacology, 2014
Matricaria recutita L. (Chamomile) has been widely used in the Tunisian traditional medicine for ... more Matricaria recutita L. (Chamomile) has been widely used in the Tunisian traditional medicine for the treatment of digestive system disorders. The present work aims to investigate the protective effects of chamomile decoction extract (CDE) against castor oil-induced diarrhea and oxidative stress in rats. The antidiarrheal activity was evaluated using castor oil-induced diarrhea method. In this respect, rats were divided into six groups: Control, Castor oil, Castor oil+Loperamide (LOP) and Castor oil+various doses of CDE. Animals were per orally (p.o.) pre-treated with CDE during 1h and intoxicated for 2 or 4h by acute oral administration of castor oil. Our results showed that CDE produced a significant dose-dependent protection against castor oil-induced diarrhea and intestinal fluid accumulation. On the other hand, we showed that diarrhea was accompagned by an oxidative stress status assessed by an increase of malondialdehyde (MDA) level and depletion of antioxidant enzyme activities as superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx). Castor oil also increased gastric and intestinal mucosa hydrogen peroxide (H2O2) and free iron levels. Importantly, we showed that chamomile pre-treatment abrogated all these biochemical alterations. These findings suggested that chamomile extract had a potent antidiarrheal and antioxidant properties in rats confirming their use in traditional medicine.
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Lipids in Health and Disease, 2013
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Papers by Abdelaziz Souli