Figure 2

Heterogeneity in fatty acid composition induces growth and division. (a) C14:1 MLVs produced daughter vesicles when fed with 5 equiv of C18:1 LUVs. (b) C14:1 MLVs fed with 5 equiv of C14:1 LUVs did not show signs of growth or division. (c) Quantification of vesicle abundance. (d) Surface area changes determined by FRET. C14:1 MLVs fed with 2.5 equiv of C18:1 LUVs showed an ~4-fold increase in surface area within the first 3 min. Images in (a) and (b) are from different fields but with identical exposure times. Red arrows indicate filamentous growth, and yellow arrows indicate freshly divided spherical vesicles. Scale bars indicate 100 μm. Flares are due to a long exposure time.