Fig. 8

A, B Identification of mice with conditional skeletal muscle deletion (Nlk^fl/flHSA-Cre) at the genome, protein (A) and mRNA levels (B) via genomic PCR, immunoblotting and real-time PCR, respectively (n=3). C, D, Mouse, leg (C) and skeletal muscle images (D) as well as corresponding graphical representation showing increased whole body and skeletal muscle weights for Nlk-deficient mice compared with wild-type mice (n=6). E Hematoxylin and eosin staining and the corresponding graphical representation showing increased skeletal muscle cell numbers in Nlk-deficient mice compared with wild-type mice. Scale bar, 500μm. F Real-time PCR showing the gene transcription levels of Egr1, Fos, Vcl, Actg2, Myl9, Tnnc1, Sm22α, MyoG, MyoD, and MHC in wild-type and Nlk-deficient mouse skeletal muscle. The real-time PCR values were normalized to the Gapdh values (n=3). G Immunoblotting showing the protein levels of Vcl and Sm22α in Nlk-deficient mouse skeletal muscle compared with wild-type mouse skeletal muscle using the indicated antibodies. Gapdh was used as a loading control. Data are representative of three independent experiments and presented as the mean±SEM. Statistical significance was analyzed by ANOVA (*p<0.05, **p<0.01, ***p<0.001, ****p<0.0001). IB immunoblotting, GAS gastrocnemius. Source data (A–G) are provided as a source data file.