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Fig. 3

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Increased ovarian cell apoptosis in Prdx4−/− mice.

a Apoptosis was analysed using an in situ TUNEL fluorescence assay (×20). In the TUNEL assay, nuclei of TUNEL-positive (apoptotic) cells were stained green. b The number of TUNEL-positive granulosa cells was compared between the two groups. Scale bars = 200 μm at ×10 magnification; scale bar = 50 μm at ×20 magnification. All data are shown as means ± SEMs, n = 5. Statistical significance: *P < 0.05.

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