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Figure 5
Luciferase reporter studies on the PLDN promoter in PMA-treated HEL cellsShown is luciferase promoter activity at 24 hours of the wildtype construct and the effect of mutating each of the six RUNX1 consensus-binding sites in the 5′ upstream region of PLDN. The ratio of firefly to Renilla luciferase activity of the wild type construct, constructs with each RUNX1 consensus site mutated, and the vector alone is shown as mean (± SEM) of three experiments.
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