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PMC full text:
Biochim Biophys Acta. Author manuscript; available in PMC 2018 May 1.
Published in final edited form as:
Biochim Biophys Acta. 2017 May; 1862(5): 452–462.
Published online 2017 Jan 31. doi: 10.1016/j.bbalip.2017.01.008

Fig. 6

An external file that holds a picture, illustration, etc. Object name is nihms911443f6.jpg

PGDHC promoted RANKL-induced osteoclastogenesis, even after the suppression of Myh9 expression, using RNA interference (RNAi) for Myh9 mRNA in vitro. A) Gene expression of Myh9 mRNA in RAW264.7 cells treated with non-silencing siRNA (NS siRNA) and siRNA specific for Myh9 mRNA; B) Western blot analysis showed nearly complete suppression of Myh9 protein expression by siRNA treatment compared to NS-siRNA treatment, both of which were performed for RANKL-stimulated RAW 264.7 cells; C) and D) TRAP staining and number of TRAP+ osteoclasts emerging in RANKL- or RANKL/PGDHC-stimulated RAW 264.7 cells reacted with NS-siRNA or siRNA-myh9, respectively. Data are expressed as mean ± SD, and representative of three independent experiments is shown. * p < 0.05; ** p < 0.01; *** p < 0.001.

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