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Published online 2011 Jul 21. doi: 10.1186/1472-6750-11-76
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Figure 7

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L19 IgG-DnaEN after PTS. Analysis by reducing SDS-PAGE (Coomassie stained). Purified fusion protein was treated with 5 mM DTT and 50 μM DnaEC peptide for 22h at RT. After dialysis, trans-spliced L19 IgG was purified by His tag affinity chromatography (1: L19 IgG-DnaEN before PTS, 2: after PTS, before purification, 3: after purification, HC: heavy chain, LC: light chain, * product not further investigated).

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